Culture medium for metal chronic toxicity experiment and application thereof

By providing a medium for experiments for chronic toxicity of metals without EDTA, the problem of existing culture media interfering with the toxicity experiment of rare earth elements is solved, the accuracy and reliability of the experiment are improved, and the chronic toxicity and ecological risks of rare earth elements can be more accurately evaluated.

CN119699243AActive Publication Date: 2025-03-28SUN YAT SEN UNIV
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Patent Information

Application Number
CN202411971786.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-30
Publication Date
2025-03-28
Estimated Expiration
2044-12-30

AI Technical Summary

Technical Problem

The existing large-scale chronic toxicity experimental medium contains chelating agents such as EDTA, which interferes with the accuracy and reliability of rare earth elements and the bioavailability experiments.

Method used

Provide a medium for experiments for chronic toxicity of metals without EDTA or other chelating agents, including metal ions, Se4+, HCO3-, SO42-, Cl- and vitamins, which are suitable for long-term cultivation of large-scale sterilization to reduce the impact of medium composition on the metal evaluation results.

Benefits of technology

It improves the accuracy and reliability of rare earth elements toxicity and biological effectiveness experiments, can more accurately determine the chronic toxicity or biological effectiveness of rare earth elements, explore their long-term ecological risks, and provide reference for the formulation of metal environmental standards and scientific environmental decision-making.

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Abstract

The invention belongs to the technical field of zooplankton culture, and particularly relates to a culture medium for a metal chronic toxicity experiment and application of the culture medium. The culture medium provided by the invention does not contain EDTA (Ethylene Diamine Tetraacetic Acid) or other chelating agents, can meet the requirements of normal reproduction and growth of model organisms, is suitable for long-term culture of the model organisms, is beneficial to reducing the influence of components of the culture medium on evaluation and determination results of the bioavailability and toxicity of metals, improves the accuracy and reliability of the evaluation results, and has a wide application prospect. Therefore, the chronic toxicity or bioavailability of the rare earth elements can be more accurately determined, the long-term ecological risk of the rare earth elements can be accurately explored, and a new reference approach is provided for metal environment standard formulation and scientific environment decision making.
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