Biopesticide for preventing and treating soft rot of radish

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a biopesticide for preventing and treating soft rot of radishes. The biopesticide for preventing and treating radish soft rot is composed of an effective component and an auxiliary component, and the effective component is formed by compounding amisulbrom and berberine or aureonucleomycin. According to the invention, amisulbrom and berberine or aureonucleomycin are compounded, so that a synergistic interaction effect is achieved, and compared with a single agent, the control effect on soft rot of radish can be improved, the dosage of the agent is reduced, and the control cost and pesticide residues are reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a biological pesticide for preventing and controlling radish soft rot. Background Art

[0002] Radish soft rot is a common bacterial disease of radish caused by infection with Pectobacterium carotovorum subsp. carotovorum, which can cause radish to completely lose its commercial and edible value. Soft rot mainly harms the fleshy roots, petioles or leaves of radish. Root infection often begins from insect damage or mechanical wounds at the root tip, and water-soaked brown spots appear, which spread upward from the diseased part, causing the roots to become dark brown, soft, rotten and ulcerated, and appearing slimy and muddy. The petioles or leaves become infected, and the base of the petioles becomes water-soaked and softened. From the base upward, the leaves turn yellow and rot, and fall off downward. The boundary between diseased and healthy tissues is obvious, and the diseased part often oozes juice with a distinct unpleasant odor.

[0003] Before the disease occurs or in the early stage, you can use Zhongshengmycin, kasugamycin, zinc thiazole, thiazolone or chlorobromoisocyanuric acid spray to control radish soft rot. However, there are relatively few pesticides on the market to control bacterial diseases, and with the long-term and large-scale use of existing conventional pesticides, pathogens have developed varying degrees of drug resistance, resulting in a decrease in control effects year by year.

[0004] Indazolesulfanil is a triazolesulfonamide contact fungicide, belonging to the Qi (ubiquinone reductase) site fungal respiration inhibitor, mainly by inhibiting the ubiquinone reductase of fungi to block the respiratory chain of fungi, and finally causing fungal death. In addition, Qi site inhibition can also interfere with the structure and function of fungal cell membranes, increase the permeability of cell membranes, make the substances in cells leak out, and further accelerate the death of fungal cells. In the prior art, it is also disclosed that indazolesulfanil has a preventive effect on bacterial diseases, such as indazolesulfanil and organic copper compounds such as thiocarbamide, thiocarbamide, nonyl copper or quinoline copper, or with tetramycin, Zhongshengmycin and other antibiotic compounds to show synergistic effect on cucumber bacterial angular spot. The inventor found through indoor experiments that indazolesulfanil and berberine or aureomycin compound to carrot soft rot Erwinia soft rot subspecies causing radish soft rot showed synergistic effect, and there is no relevant report in this regard.

[0005] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0006] The object of the present invention is to provide a biological pesticide for preventing and controlling radish soft rot, which can improve the prevention and control effect of radish soft rot compared with a single agent.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A biological pesticide for preventing and controlling radish soft rot consists of an active ingredient and an auxiliary ingredient, wherein the active ingredient is a compound of indazolesulfamide and berberine or aureomycin.

[0009] Furthermore, the mass ratio of indazolesulfamide to berberine is 1-15:30-1.

[0010] Furthermore, the mass ratio of indazolesulfamide to aureomycin is 1-5:20-1.

[0011] Furthermore, the auxiliary component is selected from one or more of a dispersant, a wetting agent, a solvent, an emulsifier, an inert filler, a disintegrant, a thickener, an antifreeze agent, a defoaming agent, a synergist and a preservative.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] (1) The present invention combines indazolesulfamide with berberine or aureomycin to have a synergistic effect. Compared with a single agent, it can improve the control effect of radish soft rot, help reduce the dosage of the agent, and reduce the control cost and pesticide residues.

[0014] (2) The action mechanism of indazolesulfamide in the biological pesticide of the present invention is different from that of berberine or aureomycin, which can increase the action sites, delay the development of drug resistance in pathogens, and help to extend the renewal cycle of the agent. DETAILED DESCRIPTION

[0015] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0016] Example :Indoor biological activity test of indazolesulfamide compound

[0017] 1. Test pathogens

[0018] Erwinia carotovora subsp. carotovora was isolated from diseased radish samples with typical symptoms of radish soft rot.

[0019] Pick a single colony of the test pathogen and place it in 20 mL NB liquid medium. Culture the test pathogen at 28°C and 220 rpm with shaking until the logarithmic growth phase. Then dilute the bacterial solution with NB liquid medium to 5×10 7 cfu / mL of bacterial suspension.

[0020] 2. Culture medium

[0021] NB medium: polypeptone 5 g / L, yeast powder 1 g / L, beef extract 3 g / L, sucrose 15 g / L, agar 15 g / L, and 10 mol / L sodium hydroxide solution to adjust the pH to 7.0.

[0022] 3. Test reagents

[0023] 97.5% indazolesulfamide, 98% berberine, 94% aureomycin.

[0024] 4. Test methods

[0025] The turbidity method was used. After the test agent was dissolved, it was diluted with 0.1% Tween-80 aqueous solution to form a single-dose mother solution. Multiple groups of ratios were set, and the single-dose mother solution and the ratio mixture were diluted with 0.1% Tween-80 aqueous solution to form 5 mass concentration gradients. The drug solution was prepared and used immediately. Under sterile conditions, 2mL of the drug solution and 30mL of NB liquid culture medium were added, and the treatment with an equal amount of 0.1% Tween-80 aqueous solution was set as a blank control. Each treatment was set up with 4 replicates. Then 100μL of the test pathogen suspension was inoculated into the treated NB liquid culture medium and placed at 28°C and 200rpm for shaking culture.

[0026] Before starting the culture, the absorbance value (OD) of each treatment at 600 nm was measured using a UV spectrophotometer. 600 ), when the blank control treatment reached the logarithmic growth phase, the OD of each treatment was measured again. 600 , calculate the growth inhibition rate of the test pathogens.

[0027]

[0028] 4. Data Analysis

[0029] DPS was used to perform regression analysis on the logarithmic value of the drug concentration and the probability value of the growth inhibition rate to calculate the EC of each treatment drug. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method. The synergistic effect of mixed drugs was evaluated according to the co-toxicity coefficient (CTC), that is, CTC≤80 is antagonistic, 80<CTC<120 is additive, and CTC≥120 is synergistic. The experimental results are shown in Table 1-2.

[0030] Table 1 The results of toxicity test of indazolesulfamide and berberine against Erwinia carotovora subsp.

[0031] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Indazolamide 16.035 100.000 -- -- Berberine 3.741 428.629 -- -- Indazolesulfamide 1: berberine 30 3.134 511.646 418.028 122.395 Indazolesulfamide 1: berberine 20 1.250 1282.800 412.980 310.621 Indazolesulfamide 1: berberine 10 1.569 1021.989 398.753 256.296 Indazolesulfamide 1: berberine 5 2.062 777.643 373.857 208.005 Indazolesulfamide 1: berberine 3 3.815 420.315 346.472 121.313 Indazolesulfamide 1: berberine 1 4.911 326.512 264.314 123.532 Indazolesulfamide 3: berberine 1 5.517 290.647 182.157 159.558 Indazolesulfamide 5: berberine 1 3.087 519.436 154.771 335.615 Indazolesulfamide 10: berberine 1 7.105 225.686 129.875 173.771 Indazolesulfamide 15: berberine 1 9.617 166.736 120.539 138.325

[0032] As shown in Table 1, when indazolesulfamide and berberine are compounded in a mass ratio of 1-15:30-1, the co-toxicity coefficient for inhibiting the growth of radish soft rot bacteria Erwinia carotovora subspecies is between 121.313-335.615, all greater than 120, showing a synergistic effect. This shows that the compounding of indazolesulfamide and berberine in the present invention can improve the control effect of radish soft rot.

[0033] Table 2 The results of toxicity test of indazolesulfamide and aureomycin against Erwinia carotovora subsp.

[0034]

[0035]

[0036] As shown in Table 2, when indazolesulfamide and aureomycin are compounded at a mass ratio of 1-5:20-1, the co-toxicity coefficient for inhibiting the growth of radish soft rot bacteria Erwinia carotovora subspecies is between 121.310 and 199.431, all greater than 120, showing a synergistic effect. This indicates that the compounding of indazolesulfamide and aureomycin in the present invention can improve the control effect of radish soft rot.

[0037] In summary, the present invention combines indazolesulfamide with berberine or aureomycin, which has a synergistic effect. Compared with a single agent, it can improve the control effect of radish soft rot, help reduce the dosage of the agent, and reduce the control cost and pesticide residues.

Claims

1. A biological pesticide for preventing and controlling radish soft rot, characterized in that: The invention is composed of effective ingredients and auxiliary ingredients, wherein the effective ingredients are compounded by indazolesulfamide and berberine or aureomycin.

2. The biological pesticide for preventing and controlling radish soft rot according to claim 1, characterized in that: The mass ratio of indazolesulfamide to berberine is 1-15:30-1.

3. The biological pesticide for preventing and controlling radish soft rot according to claim 1, characterized in that: The mass ratio of indazolesulfamide to aureomycin is 1-5:20-1.

4. The biological pesticide for preventing and controlling radish soft rot according to claim 1, characterized in that: The auxiliary components are selected from one or more of dispersants, wetting agents, solvents, emulsifiers, inert fillers, disintegrants, thickeners, antifreeze agents, defoamers, synergists and preservatives.

Images