Antiviral composition containing nano-zinc, preparation and application thereof

Abstract

The invention discloses an antiviral composition containing nano-zinc, a preparation and application thereof, and belongs to the technical field of biopesticides. The antiviral composition is prepared from nano zinc and amino-oligosaccharin. Nano-zinc can improve the growth index and photosynthesis of tomato plants and the activity of antioxidant defensive enzyme, and reduce the levels of malondialdehyde and active oxygen at the same time. The amino-oligosaccharin can be used as an active signal molecule in the agricultural field to cause the plant to generate resistance, and in addition, the amino-oligosaccharin can promote plant cell activation and stimulate plant growth. The nano-zinc and the amino-oligosaccharin are reasonably compounded and screened, so that the nano-zinc and the amino-oligosaccharin can achieve a mutual synergistic effect within a certain range, the use amount of active ingredients can be reduced, the pesticide use cost can be reduced, and the prevention and treatment effect on plant virus diseases can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biological pesticides, and specifically relates to an antiviral composition containing nano zinc, a preparation and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Viral diseases, also known as "plant cancer", are the second largest plant disease after fungal diseases. Many plants are infected by plant viruses and cause viral diseases. Plant viruses quickly transfer in the vascular bundle along the flow direction of plant nutrients, causing disease throughout the body, resulting in symptoms such as discoloration, necrosis and deformity. According to statistics, there are 121 genera and 1,440 species of viruses that infect plants. Currently, more than 400 species of plants can be infected by plant viruses, resulting in a sharp decline in crop yield and quality. The economic losses caused each year are quite serious, restricting the healthy and sustainable development of agriculture.

[0004] Developing new plant varieties with genetic resistance and inducing plant resistance are the most effective strategies for preventing and controlling viral diseases. However, the cycle of developing resistant varieties and inducing plant resistance is long, and plant viruses are very easy to mutate, so it is difficult to promote the use of these two strategies to prevent and control plant viral diseases. Chemical control is currently the most widely used strategy for preventing and controlling viral diseases, but no very effective agents have been found for the prevention and control of plant viral diseases. The mechanism of action of chemical control is mainly to inhibit viral infection, inhibit viral proliferation and induce plant resistance. The prevention and control of viral diseases is mainly reflected in the early prevention effect, and the effect is generally 40% to 80%, while the treatment effect is poor. Therefore, it is urgent to develop new agents for the prevention and control of viral diseases.

[0005] Sofy AR et al. (Sofy AR, Sofy MR, Hmed AA, et al. Ameliorating the Adverse Effects of Tomato mosaic tobamovirus Infecting Tomato Plants in Egypt by Boosting Immunity in Tomato Plants Using Zinc Oxide Nanoparticles[J]. Molecules, 2021, 26(5): 1337. DOI: 10.3390 / molecules26051337) reported that nano zinc improved the resistance of tomato plants to mosaic virus and is a safe and economical potential active substance against tomato mosaic virus.

[0006] Aminoligosaccharides can prevent and treat a variety of plant fungal diseases and viral diseases. As a biological pesticide, it has multiple mechanisms in disease prevention and disease resistance. In addition to being an active signal molecule that induces resistance in plants, it can also be used as a plant growth regulator to promote plant cell activation and stimulate plant growth, and has the dual effects of medicine and fertilizer. Aminoligosaccharides can delay or alleviate symptoms, but cannot effectively eradicate viruses, and the effect of use in the short term is not as obvious as that of chemical preparations, nor as fast as that of chemical pesticides. The use of a reasonable composite composition and timely medication are important factors for giving full play to the preventive and therapeutic effects. Patent CN106818753A discloses an antiviral composition containing wolfsbane toxin, which combines wolfsbane toxin and aminooligosaccharides in a weight ratio of 1:30-30:1. The two active ingredients synergize with each other, reduce the amount of active ingredients used, reduce the cost of medication, and improve the preventive and therapeutic effects of tomato virus diseases. However, there is currently no report on the use of a composite composition of nano zinc and aminooligosaccharides for plant virus disease prevention and control. Summary of the invention

[0007] In order to solve the shortcomings of the prior art, the purpose of the present invention is to provide an antiviral composition, preparation and application thereof containing nano zinc. The antiviral composition provided by the present invention is composed of nano zinc and amino oligosaccharides, which have a synergistic effect in preventing and treating plant viral diseases in a certain proportion and have good safety.

[0008] In order to achieve the above object, the technical solution of the present invention is:

[0009] The first aspect of the present invention provides an antiviral composition, which is composed of nano zinc and amino oligosaccharides, and the mass ratio of the nano zinc to the amino oligosaccharides is 5:4 to 5:10.

[0010] The present invention has found through a large number of experiments that nano zinc and amino oligosaccharides have a synergistic effect on the prevention and treatment of viral diseases in the above ratio, which can reduce the amount of active ingredients used and improve the prevention and treatment effect.

[0011] In some embodiments of the present invention, the mass ratio of the nano zinc to the amino oligosaccharide is 5: 7. Under this ratio, the antiviral composition has the best control effect on plant viral diseases.

[0012] The second aspect of the present invention provides an antiviral preparation, which comprises the above-mentioned antiviral composition, wherein the antiviral composition accounts for 5-20 wt % of the antiviral preparation.

[0013] The antiviral preparation provided by the present invention has the advantages of safety, low pesticide residue, stable efficacy, and not easy to produce resistance. It should be noted that the antiviral composition can be used as the only active ingredient of the antiviral preparation, and can also be compounded with other active ingredients to improve the prevention and control effect of the antiviral preparation on plant viral diseases.

[0014] In some embodiments of the present invention, the antiviral composition accounts for 6-15 wt % of the antiviral formulation.

[0015] In some embodiments of the present invention, the dosage form of the preparation includes, but is not limited to, one of a suspension concentrate (SC), a soluble concentrate (SL), an emulsion in water (EW) and a microemulsion (ME).

[0016] In some embodiments of the present invention, the antiviral formulation further comprises a surfactant, a defoaming agent and water;

[0017] Calculated by mass percentage, the antiviral preparation comprises 5-20wt% of an antiviral composition, 8-15wt% of a surfactant, 0.001-0.02wt% of a defoaming agent, and the balance is water.

[0018] In some embodiments of the present invention, the surfactant includes one of lauryl alcohol polyoxyethylene ether, octanol polyoxyethylene ether and alkylphenol polyoxyethylene ether, preferably lauryl alcohol polyoxyethylene ether.

[0019] In some embodiments of the present invention, the defoamer comprises one of an organosilicon defoamer, a polyether defoamer and a mineral oil defoamer, preferably an organosilicon defoamer.

[0020] In some embodiments of the present invention, the antiviral preparation includes, by mass percentage, 10-20 wt % of the antiviral composition, 10-12 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water.

[0021] In some embodiments of the present invention, the antiviral preparation includes 10.8-18 wt % of the antiviral composition, 10-12 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water.

[0022] In some embodiments of the present invention, the antiviral preparation includes 10.8 wt % of the antiviral composition, 10 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water.

[0023] In some embodiments of the present invention, the antiviral preparation includes 12 wt % antiviral composition, 10 wt % lauryl alcohol polyoxyethylene ether, 0.01 wt % organosilicon defoaming agent, and the balance water.

[0024] In some embodiments of the present invention, the antiviral preparation includes 14.4 wt % of the antiviral composition, 11 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water.

[0025] In some embodiments of the present invention, the antiviral preparation includes 18 wt % antiviral composition, 12 wt % lauryl alcohol polyoxyethylene ether, 0.01 wt % organosilicon defoaming agent, and the balance water.

[0026] In some embodiments of the present invention, the antiviral preparation includes, by mass percentage, 14.4 wt % antiviral composition, 11 wt % lauryl alcohol polyoxyethylene ether, 0.01 wt % silicone defoamer, and the remainder is water; the mass ratio of nano zinc to amino oligosaccharide is 5:7.

[0027] The third aspect of the present invention provides a use of the above-mentioned antiviral composition or the above-mentioned antiviral preparation in preventing and controlling plant viral diseases.

[0028] In some embodiments of the present invention, the plant virus disease includes any one of tomato virus disease, potato virus disease, pepper virus disease, cucumber virus disease and tobacco virus disease, preferably tomato virus disease.

[0029] The beneficial effects of the present invention are:

[0030] The present invention provides an antiviral composition containing nano zinc, which is composed of nano zinc and amino oligosaccharides. Nano zinc can improve the growth index, photosynthesis and antioxidant defense enzyme activity of tomato plants, and reduce the levels of malondialdehyde and reactive oxygen. Amino oligosaccharides can be used as active signal molecules in the agricultural field to cause plants to produce resistance, and can also promote plant cell activation and stimulate plant growth. The present invention reasonably mixes and screens nano zinc and amino oligosaccharides so that they can achieve a synergistic effect within a certain range, thereby reducing the amount of active ingredients used, reducing the cost of medication, and improving the prevention and control effect.

[0031] The antiviral composition provided by the present invention has safe ingredients, low pesticide residues, stable drug efficacy, is not easy to produce resistance, can delay the development of drug resistance of viral diseases, and can extend the service life of active ingredients of pesticides. DETAILED DESCRIPTION

[0032] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0033] Example 1

[0034] An antiviral soluble solution containing nano zinc, the raw material composition of which is as follows in terms of mass percentage:

[0035] Nano zinc: 6wt%

[0036] Aminooligosaccharides: 4.8wt%

[0037] Lauryl alcohol polyoxyethylene ether: 10wt%

[0038] Silicone defoamer: 0.01wt%

[0039] Water: make up to 100 wt%.

[0040] The preparation method of the nano zinc-containing antiviral soluble solution is as follows: according to the formula, the active ingredients and various auxiliary agents, solvents, etc. are uniformly mixed in a mixing kettle according to corresponding proportions to obtain the solution.

[0041] Example 2

[0042] An antiviral soluble solution containing nano zinc, the raw material composition of which is as follows in terms of mass percentage:

[0043] Nano zinc: 6wt%

[0044] Aminooligosaccharides: 6wt%

[0045] Lauryl alcohol polyoxyethylene ether: 10wt%

[0046] Silicone defoamer: 0.01wt%

[0047] Water: make up to 100 wt%.

[0048] The preparation method of the antiviral soluble solution containing nano zinc is the same as that of Example 1.

[0049] Example 3

[0050] An antiviral soluble solution containing nano zinc, the raw material composition of which is as follows in terms of mass percentage:

[0051] Nano zinc: 6wt%

[0052] Aminooligosaccharides: 8.4wt%

[0053] Lauryl alcohol polyoxyethylene ether: 11wt%

[0054] Silicone defoamer: 0.01wt%

[0055] Water: make up to 100 wt%.

[0056] The preparation method of the antiviral soluble solution containing nano zinc is the same as that of Example 1.

[0057] Example 4

[0058] An antiviral soluble solution containing nano zinc, the raw material composition of which is as follows in terms of mass percentage:

[0059] Nano zinc: 6wt%

[0060] Aminooligosaccharides: 12wt%

[0061] Lauryl alcohol polyoxyethylene ether: 12wt%

[0062] Silicone defoamer: 0.01wt%

[0063] Water: make up to 100 wt%.

[0064] The preparation method of the antiviral soluble solution containing nano zinc is the same as that of Example 1.

[0065] Experimental Example 1 Field efficacy test of the antiviral soluble solution containing nano zinc obtained in Examples 1-4 for controlling tomato virus disease

[0066] Test agent: antiviral soluble solution containing nano zinc prepared in Example 1-4.

[0067] Control agents: 6% nano zinc soluble solution (self-prepared), 5% amino oligosaccharide aqueous solution (Jiangxi Zhengbang Crop Protection Co., Ltd.).

[0068] Tested crops: Tomato, Millennium

[0069] Target of prevention and control: Tomato virus disease

[0070] Test site conditions: The test site is located in the tomato greenhouse of Tunxi New Village, Luocheng Street, Shouguang City, Shandong Province. The soil type is loam with medium fertility. Tomato virus disease has occurred in the test site every year. The cultivation conditions and management measures of all test plots are consistent.

[0071] Experimental design: Each plot has an area of ​​15m 2 , repeated 3 times, and the plots were arranged randomly.

[0072] Experimental investigation and calculation method:

[0073] The pesticide was applied three times on May 8, May 15 and May 22, 2024. The disease index was investigated 7 days after the application and the control effect was calculated. During the investigation, 5 random sampling points were taken in each plot, and 5 plants were investigated at each point. The total number of investigated plants and the number of diseased plants at each level were recorded in units of plants. The disease classification standards are as follows:

[0074] Grade 0: no symptoms;

[0075] Level 1: clear veins, light mosaic;

[0076] Level 3: mosaic of heart leaves and middle leaves;

[0077] Level 5: The heart leaves and middle leaves are mosaic, a few leaves are deformed, wrinkled or the plant is slightly dwarfed;

[0078] Level 7: Severe mosaic, most leaves are deformed, shrunken or the plant is dwarfed;

[0079] Level 9: Severe mosaic, obvious leaf deformity and linear leaves, severe dwarfing of the plant, and even death.

[0080] Disease index = 100 × ∑ (number of diseased leaves at each level × representative value of each level) / (total number of leaves surveyed × 9)

[0081] Control effect (%) = (disease index of control group after application - disease index of treatment group after application) / disease index of control group after application × 100

[0082] Table 1 Field efficacy test results of the test and control agents for the prevention and treatment of tomato virus diseases

[0083]

[0084]

[0085] As shown in Table 1, the nano-zinc-containing antiviral soluble solution of Examples 1-4 has a higher control effect on tomato virus disease than the control single agent. It can be seen that the nano-zinc-containing antiviral composition and antiviral preparation provided by the present invention show a good control effect on tomato virus disease. By comparing the treatment groups 2, 3, and 4, it can be seen that the control effect of different dosages is different, and the control effect increases with the increase of the dosage. By comparing the treatment groups 1, 2, 5, and 6, it can be seen that the ratio of nano-zinc and amino-oligosaccharides affects the control effect of the antiviral preparation. When the mass ratio of nano-zinc and amino-oligosaccharides is 5:7, the nano-zinc-containing antiviral soluble solution has a good control effect, and at a low dosage, it reaches a control effect of more than 90%. Finally, within the above-mentioned medication range, it has not been found that the nano-zinc and amino-oligosaccharides antiviral composition has any phytotoxicity to tomato plants, indicating that the antiviral composition of the present invention has good safety.

[0086] In summary, the antiviral composition and antiviral preparation of the present invention have the characteristics of high safety and good prevention effect, and have great application potential.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An antiviral composition, characterized in that: The invention is composed of nano zinc and amino oligosaccharide, wherein the mass ratio of the nano zinc to the amino oligosaccharide is 5:4 to 5:

10.

2. The antiviral composition according to claim 1, characterized in that The mass ratio of the nano zinc to amino oligosaccharides is 5:

7.

3. An antiviral preparation, characterized in that: The preparation comprises the antiviral composition according to any one of claims 1 or 2, wherein the antiviral composition accounts for 5-20 wt% of the antiviral preparation.

4. The antiviral preparation according to claim 3, characterized in that The antiviral composition accounts for 6-15 wt % of the antiviral formulation.

5. The antiviral preparation according to claim 3, characterized in that The dosage form of the preparation includes one of a suspension, a soluble solution, an aqueous emulsion and a microemulsion.

6. The antiviral preparation according to claim 3, characterized in that The antiviral preparation also includes a surfactant, a defoaming agent and water; Calculated by mass percentage, the antiviral preparation comprises 5-20wt% of an antiviral composition, 8-15wt% of a surfactant, 0.001-0.02wt% of a defoaming agent, and the balance is water.

7. The antiviral preparation according to claim 6, characterized in that The surfactant includes one of lauryl alcohol polyoxyethylene ether, octanol polyoxyethylene ether and alkylphenol polyoxyethylene ether, preferably lauryl alcohol polyoxyethylene ether; Preferably, the defoamer includes one of an organosilicon defoamer, a polyether defoamer and a mineral oil defoamer, preferably an organosilicon defoamer.

8. The antiviral preparation according to claim 7, characterized in that In terms of mass percentage, the antiviral preparation comprises 10-20wt% of an antiviral composition, 10-12wt% of lauryl alcohol polyoxyethylene ether, 0.01wt% of an organosilicon defoamer, and the balance is water; Preferably, the antiviral preparation comprises 10.8-18wt% antiviral composition, 10-12wt% lauryl alcohol polyoxyethylene ether, 0.01wt% organosilicon defoamer, and the balance water; Preferably, the antiviral preparation comprises 10.8 wt % of the antiviral composition, 10 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water; Preferably, the antiviral preparation comprises 12 wt% of the antiviral composition, 10 wt% of lauryl alcohol polyoxyethylene ether, 0.01 wt% of an organosilicon defoamer, and the balance is water; Preferably, the antiviral preparation comprises 14.4wt% antiviral composition, 11wt% lauryl alcohol polyoxyethylene ether, 0.01wt% organosilicon defoamer, and the balance water; Preferably, the antiviral preparation comprises 18 wt % of the antiviral composition, 12 wt % of lauryl alcohol polyoxyethylene ether, 0.01 wt % of an organosilicon defoaming agent, and the balance is water.

9. The antiviral preparation according to claim 7, characterized in that In terms of mass percentage, the antiviral preparation includes 14.4wt% of an antiviral composition, 11wt% of lauryl alcohol polyoxyethylene ether, 0.01wt% of an organosilicon defoaming agent, and the remainder is water; the mass ratio of nano zinc to amino oligosaccharide is 5:

7.

10. Use of the antiviral composition according to any one of claims 1 or 2 or the antiviral preparation according to any one of claims 3 to 9 in preventing and treating plant viral diseases; Preferably, the plant virus disease includes any one of tomato virus disease, potato virus disease, pepper virus disease, cucumber virus disease and tobacco virus disease, preferably tomato virus disease.

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