Micro-ecological compound preparation for feeding lambs and preparation method of micro-ecological compound preparation

Abstract

The invention discloses a micro-ecological compound preparation for feeding lambs and a preparation method thereof, and the preparation method comprises the following steps: activating and culturing probiotic strains, freeze-drying to obtain probiotic powder, and preparing the probiotic powder and lactose into probiotic microspheres; the preparation method comprises the following steps: dispersing probiotic microspheres in a guar gum solution, selecting a konjac glucomannan solution as an external phase, sucking the guar gum solution, simultaneously immersing the outside of an injection tube in the external phase, controlling the flow rates of the external phase and the guar gum solution, shearing the guar gum solution and the probiotic microspheres into a spherical microsphere-double-layer gel structure by the external phase at the outlet of the injection tube, and carrying out freeze-drying to obtain the konjac glucomannan-containing probiotic microspheres. Collecting through a collecting pipe, and freeze-drying to obtain a micro-ecological compound preparation inner core; and coating the inner core of the micro-ecological compound preparation with soybean meal and starch to obtain the micro-ecological compound preparation. According to the invention, a micro-hydraulic control technology is utilized, and the probiotic microspheres are coated by konjac glucomannan and a guar gum solution, so that the micro-ecological compound preparation for feeding lambs, which is high in stress resistance, is prepared.

Description

Technical Field

[0001] The invention belongs to the technical field of biological agriculture and probiotic preparation, and more specifically, relates to a microecological composite preparation for lamb feed and a preparation method thereof. Background Art

[0002] Tibetan goats are excellent meat goats with strong adaptability and excellent meat quality. They are rich in high protein, low fat, low cholesterol and a variety of amino acids. The breeding of meat goats in Tibetan areas has always been mainly based on grazing. Due to natural conditions, the dry grass period is long and the supply of forage is unbalanced. Insufficient forage, single feed, especially lack of protein and vitamins, are the main reasons why sheep in Tibetan areas do not estrus. Therefore, according to the specific nutritional needs of Tibetan meat goats at different physiological stages, it is the key research direction for technicians in this field to carry out research on nutritional regulation and precise feed configuration technology, actively promote the mode of shed feeding or wide seasonal semi-shed feeding, optimize feed ratio, develop microecological preparations, and focus on improving the reproductive performance of breeding goats. Carry out early supplementary feeding of lambs, integrate early lamb cultivation technology by studying the time and amount of supplementary feeding, lay a solid foundation for the smooth transition of lamb weaning, reduce stress and mortality after weaning, improve the survival rate of weaned lambs, and achieve more lambs, more survival, and more output.

[0003] At the same time, with the expansion of the scale of breeding and the improvement of the degree of intensification in Tibetan areas, lambs are also facing more and more health challenges, such as intestinal diseases, decreased immunity, etc. In order to meet these challenges and improve the survival rate of weaned lambs and breeding benefits, more and more breeders have begun to use probiotic preparations in lamb breeding.

[0004] Probiotic preparations are feed additives containing active probiotics. They regulate the balance of intestinal microecology, promote the growth of beneficial bacteria, and inhibit the reproduction of harmful bacteria, thereby improving the immunity, digestion ability and overall health of animals. In lamb farming, the application of probiotic preparations has significant advantages. First, the intestinal development of lambs is not yet complete, and their ability to digest and absorb food is relatively weak. The active probiotics in probiotic preparations can decompose the complex components in food, improve the utilization rate of feed, and promote the growth and development of lambs. Secondly, after weaning, lambs are prone to intestinal stress reactions due to changes in feed and feeding environment, leading to the occurrence of diseases such as diarrhea. Probiotic preparations can regulate the balance of intestinal microecology, enhance the barrier function of intestinal mucosa, reduce the invasion of harmful bacteria, and thus reduce the incidence of intestinal diseases in lambs. In addition, probiotic preparations can also improve the immunity of lambs and enhance their ability to resist diseases. Probiotics stimulate the development and function of the intestinal immune system, improve the lambs' ability to identify and eliminate pathogens, and reduce the occurrence and spread of diseases.

[0005] As people pay more and more attention to food safety and animal welfare, the application of probiotic preparations in lamb farming has also received widespread attention. More and more research and practice have proved that the rational use of probiotic preparations can not only improve the health level and growth performance of lambs, but also reduce the use of antibiotics, reduce breeding costs, and improve breeding benefits. Therefore, feeding lambs with probiotic preparations has become an important breeding technology and management method in Tibetan animal husbandry and even in modern animal husbandry. Through the scientific and rational use of probiotic preparations, the healthy growth of lambs can be promoted, breeding benefits and product quality can be improved, and greater contributions can be made to the development of animal husbandry.

[0006] Then, how to enhance the stress resistance of probiotic preparations and microecological compound preparations and increase the number of live bacteria is still a difficult problem in lamb farming. In order to ensure the survival of the bacteria in microecological preparations, the current common storage method is low temperature and light-proof conditions. At the same time, the effective storage time is also short, and some cannot even exceed 15 days, which increases the cost of lamb farming; therefore, in order to reduce the cost of lamb farming, it is necessary to further improve the stress resistance of microecological compound preparations for lamb feed, increase the number of live bacteria, and extend the shelf life of microecological preparations. Summary of the invention

[0007] An object of the present invention is to solve at least the above problems and / or disadvantages and to provide at least the advantages which will be described hereinafter.

[0008] In order to achieve these purposes and other advantages according to the present invention, a method for preparing a microecological composite preparation for lamb feeding is provided, comprising the following steps:

[0009] Step 1: activating and culturing the probiotic strains, freeze-drying them to obtain probiotic powder, and preparing probiotic microspheres by mixing the probiotic powder with lactose;

[0010] Step 2, step 2, dispersing the probiotic microspheres in the guar gum solution, selecting konjac glucomannan solution as the external phase, using an injection needle to absorb the guar gum solution of the dispersed oil probiotic microspheres, pumping the guar gum solution into the injection tube through a syringe pump, while the outside of the injection tube is immersed in the external phase, controlling the flow rate of the external phase and the guar gum solution, at the outlet of the injection tube, the guar gum solution and the probiotic microspheres are sheared by the external phase into a spherical microsphere-double-layer gel structure, collected by a collection tube, and freeze-dried to obtain a microecological composite preparation core;

[0011] Step 3: Use soybean powder and starch to coat the inner core of the microecological composite preparation to obtain the microecological composite preparation.

[0012] Preferably, in the step 1, the probiotic powder comprises one of Bacillus subtilis powder, Lactobacillus acidophilus powder or Lactobacillus reuteri powder.

[0013] Preferably, in the step 1, the probiotic powder is a mixed powder of Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri powder, and the mass ratio of the Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri is 1-3:1-3:1-3.

[0014] Preferably, in step 1, the mass ratio of probiotic powder to lactose is 2-10:1-8.

[0015] Preferably, in the step 1, the specific method of preparing probiotic microspheres from probiotic powder and lactose comprises:

[0016] S1. Dissolve lactose and polyethylene glycol in deionized water, heat to 40-60° C. and stir magnetically until completely dissolved to obtain a precursor solution; wherein the ratio of lactose, polyethylene glycol to deionized water is 1-8 g: 1-3 mL: 100-300 mL;

[0017] S2, ultrasonically dispersing the probiotic powder in the precursor solution at an ultrasonic frequency of 60 to 80 kHz and an ultrasonic time of 2 to 20 min to obtain a suspension;

[0018] S3. Add Span 80 and sodium alginate solution to the suspension, and use an emulsifier to emulsify to obtain an emulsion; add calcium chloride solution to the emulsion, and stir at a speed of 200-800 rpm. After the addition is completed, stop stirring, remove the upper oil layer after standing, use a centrifuge to collect the lower precipitate, and wash it with deionized water for several times to remove the residual calcium chloride and emulsifier, and obtain the probiotic microspheres after freeze-drying.

[0019] Preferably, in S3, the concentration of the sodium alginate solution is 1-2wt%, the concentration of the calcium chloride solution is 10-40wt%, and the dosage ratio of lactose, Span 80, sodium alginate solution and calcium chloride solution is 1-8g:0.5-1g:30-50mL:5-10mL.

[0020] Preferably, in the step 2, the external phase flow rate is greater than the guar gum solution flow rate, wherein the guar gum solution flow rate is 10-30 mL / h, and the external phase flow rate is 30-70 mL / h; the concentration of the konjac glucomannan solution is 1-2 wt%, and the concentration of the guar gum solution is 0.3-0.6 wt%.

[0021] Preferably, in the step 2, the konjac glucomannan solution is replaced with a modified konjac glucomannan solution, wherein the preparation method of the modified konjac glucomannan solution comprises:

[0022] A 1-2wt% konjac glucomannan solution and a 0.3-0.6wt% sodium carboxymethylcellulose solution are prepared, the konjac glucomannan solution and the sodium carboxymethylcellulose solution are mixed, and stirred evenly to obtain a mixed solution; the mixed solution is heated to 40-80°C, kept warm for 1-3h, cooled to room temperature, and allowed to stand for 12-36h to obtain a modified konjac glucomannan solution; wherein the volume ratio of the konjac glucomannan solution to the sodium carboxymethylcellulose solution is 10:1-2.

[0023] Preferably, in step three, the mass ratio of soybean flour to starch is 2 to 5:1.

[0024] A microecological composite preparation for lamb feeding is prepared from the above-mentioned microecological composite preparation for lamb feeding.

[0025] The present invention includes at least the following beneficial effects: the present invention utilizes microfluidic control technology to coat the probiotic microspheres with konjac glucomannan and guar gum solution, and the guar gum solution and konjac glucomannan achieve a three-dimensional spherical coating without bubbles and voids on the probiotic microspheres, forming a good protective effect on the probiotic powder, so that the prepared microecological composite preparation for lamb feeding has excellent stress resistance. The microecological composite preparation for lamb feeding prepared by the present invention can be stored for a long time, especially in a wide temperature range of -18°C to 37°C, the number of live probiotics in the probiotic microspheres can remain active for a long time; at the same time, the microecological composite preparation for lamb feeding prepared by the present invention also has a strong resistance to lamb rumen gastric acid.

[0026] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the injection tube and collection tube for preparing the inner core of the microecological composite preparation using microfluidic control technology in step 2 of Example 1. DETAILED DESCRIPTION

[0028] The present invention is described in further detail below so that those skilled in the art can implement it according to the description.

[0029] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0030] In each embodiment,

[0031] Example 1

[0032] This embodiment provides a method for preparing a microecological composite preparation for lamb feeding, comprising the following steps:

[0033] Step 1: After activating and culturing the Bacillus subtilis species, freeze-drying to obtain Bacillus subtilis powder, the freeze-drying temperature is -10°C, and the Bacillus subtilis powder and lactose are prepared into probiotic microspheres; wherein the specific method of preparing the Bacillus subtilis powder and lactose into probiotic microspheres includes:

[0034] S1. Dissolve 10 g lactose and 30 mL polyethylene glycol in 1000 mL deionized water, heat to 60°C and stir magnetically until completely dissolved to obtain a precursor solution;

[0035] S2, ultrasonically dispersing 50 g of Bacillus subtilis powder in the precursor solution at an ultrasonic frequency of 80 kHz and an ultrasonic time of 10 min to obtain a suspension;

[0036] S3, add 5g Span 80 and 300mL 1.5wt% sodium alginate solution to the suspension, and emulsify it using an emulsifier to obtain an emulsion; add 50mL 10wt% calcium chloride solution to the emulsion, and stir at a speed of 400rpm. After the addition is completed, stop stirring, remove the upper oil layer after standing, collect the lower precipitate using a centrifuge, and wash it with deionized water for several times to remove the residual calcium chloride and emulsifier, and obtain probiotic microspheres after freeze-drying;

[0037] Step 2: Disperse 2 g of probiotic microspheres 3 in 50 mL of 0.6 wt% guar gum solution. Figure 1 As shown, a 1.5wt% konjac glucomannan solution is selected as the external phase 1, and a guar gum solution 4 of dispersed oil probiotic microspheres 3 is sucked by a syringe needle, and pumped into an injection tube 2 by a syringe pump, while the outside of the injection tube 2 is immersed in the external phase 1, and the flow rate of the guar gum solution 4 is controlled to be 0.2mL / min, and the flow rate of the external phase 1 is 1mL / min. At the outlet of the injection tube 2, the guar gum solution and the probiotic microspheres are sheared into a spherical microsphere-double-layer gel structure by the external phase 1, and are collected by a collection tube 6 and freeze-dried to obtain a microecological composite preparation inner core 5;

[0038] Step 3: Use 20g soybean powder and 10g starch to coat 2g of the inner core of the microecological composite preparation to obtain the microecological composite preparation.

[0039] Example 2

[0040] This embodiment provides a method for preparing a microecological composite preparation for lamb feeding, comprising the following steps:

[0041] Step 1: After activating and culturing Lactobacillus acidophilus, freeze-drying to obtain Lactobacillus acidophilus powder, the freeze-drying temperature is -10°C, and preparing probiotic microspheres with Lactose; wherein the specific method of preparing probiotic microspheres with Lactobacillus acidophilus powder and lactose includes:

[0042] S1. Dissolve 10 g lactose and 20 mL polyethylene glycol in 1000 mL deionized water, heat to 60°C and stir magnetically until completely dissolved to obtain a precursor solution;

[0043] S2, ultrasonically dispersing 20 g of Lactobacillus acidophilus powder in the precursor solution at an ultrasonic frequency of 80 kHz and an ultrasonic time of 10 min to obtain a suspension;

[0044] S3, add 5g Span 80 and 300mL 1.5wt% sodium alginate solution to the suspension, and emulsify it using an emulsifier to obtain an emulsion; add 50mL 10wt% calcium chloride solution to the emulsion, and stir at a speed of 400rpm. After the addition is completed, stop stirring, remove the upper oil layer after standing, collect the lower precipitate using a centrifuge, and wash it with deionized water for several times to remove the residual calcium chloride and emulsifier, and obtain probiotic microspheres after freeze-drying;

[0045] Step 2, 2g of probiotic microspheres are dispersed in 50mL of 0.6wt% guar gum solution, 1.5wt% konjac glucomannan solution is selected as the external phase, and the guar gum solution with the dispersed probiotic microspheres is absorbed by a syringe needle, and the guar gum solution is pumped into the injection tube by a syringe pump, and the outside of the injection tube is immersed in the external phase, and the guar gum solution flow rate is controlled to be 0.4mL / min, and the external phase flow rate is 1.2mL / min. At the outlet of the injection tube, the guar gum solution and the probiotic microspheres are sheared by the external phase into a spherical microsphere-double-layer gel structure, and are collected by a collection tube and freeze-dried to obtain a microecological composite preparation core;

[0046] Step 3: Use 20g soybean powder and 10g starch to coat 2g of the inner core of the microecological composite preparation to obtain the microecological composite preparation.

[0047] Example 3

[0048] This embodiment provides a method for preparing a microecological composite preparation for lamb feeding, comprising the following steps:

[0049] Step 1: After activating and culturing Bacillus subtilis, Lactobacillus acidophilus and Lactobacillus reuteri respectively, freeze-drying to obtain Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri powder, the freeze-drying temperature is -10°C, the Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri powder are mixed in a mass ratio of 1:1:1 to form probiotic powder, and the probiotic powder and lactose are prepared into probiotic microspheres; wherein the specific method of preparing the probiotic powder and lactose into probiotic microspheres comprises:

[0050] S1. Dissolve 20 g lactose and 20 mL polyethylene glycol in 1000 mL deionized water, heat to 60°C and stir magnetically until completely dissolved to obtain a precursor solution;

[0051] S2, ultrasonically dispersing 20 g of probiotic powder in the precursor solution at an ultrasonic frequency of 80 kHz and an ultrasonic time of 10 min to obtain a suspension;

[0052] S3, add 6g Span 80 and 300mL 1.5wt% sodium alginate solution to the suspension, and emulsify it using an emulsifier to obtain an emulsion; add 50mL 10wt% calcium chloride solution to the emulsion, and stir at a speed of 400rpm. After the addition is completed, stop stirring, remove the upper oil layer after standing, collect the lower precipitate using a centrifuge, and wash it with deionized water for several times to remove the residual calcium chloride and emulsifier, and obtain probiotic microspheres after freeze-drying;

[0053] Step 2, 2g of probiotic microspheres are dispersed in 50mL of 0.6wt% guar gum solution, 1.5wt% konjac glucomannan solution is selected as the external phase, the guar gum solution is absorbed by an injection needle, and the outside of the injection tube is immersed in the external phase, the guar gum solution flow rate is controlled to be 0.5mL / min, and the external phase flow rate is 1.5mL / min. At the outlet of the injection tube, the guar gum solution and the probiotic microspheres are sheared by the external phase into a spherical microsphere-double-layer gel structure, collected by a collection tube, and freeze-dried to obtain a microecological composite preparation core;

[0054] Step 3: Use 20g soybean powder and 10g starch to coat 2g of the inner core of the microecological composite preparation to obtain the microecological composite preparation.

[0055] Example 4

[0056] This embodiment provides a method for preparing a microecological composite preparation for lamb feeding, which is different from Example 1 in that in step 2, the konjac glucomannan solution is replaced with a modified konjac glucomannan solution, wherein the preparation method of the modified konjac glucomannan solution comprises:

[0057] A 1.5wt% konjac glucomannan solution and a 0.6wt% sodium carboxymethylcellulose solution are prepared, 100mL of the konjac glucomannan solution is mixed with 20mL of the sodium carboxymethylcellulose solution, and stirred evenly to obtain a mixed solution; the mixed solution is heated to 80°C, kept warm for 3h, cooled to room temperature, and allowed to stand for 12h to obtain a modified konjac glucomannan solution.

[0058] The preparation methods and process parameters of the remaining steps of this embodiment are the same as those of embodiment 1.

[0059] Example 5

[0060] This embodiment provides a method for preparing a microecological composite preparation for lamb feeding, which is different from Example 1 in that in step 2, the konjac glucomannan solution is replaced with a modified konjac glucomannan solution, wherein the preparation method of the modified konjac glucomannan solution comprises:

[0061] A 1.5wt% konjac glucomannan solution and a 0.5wt% sodium carboxymethylcellulose solution are prepared, 100mL of the konjac glucomannan solution is mixed with 10mL of the sodium carboxymethylcellulose solution, and stirred evenly to obtain a mixed solution; the mixed solution is heated to 80°C, kept warm for 3h, cooled to room temperature, and allowed to stand for 12h to obtain a modified konjac glucomannan solution.

[0062] The preparation methods and process parameters of the remaining steps of this embodiment are the same as those of embodiment 1.

[0063] Comparative Example 1

[0064] The present comparative example provides a method for preparing a microecological composite preparation for lamb feeding, wherein the probiotic microspheres prepared in step 1 of Example 1 are first immersed in a 0.6wt% guar gum solution, filtered after immersion for 10s, and then immersed in a 1.5wt% konjac glucomannan solution, and then freeze-dried at -20°C for 30min to obtain double-layer colloid-coated probiotic microspheres, and 20g of soybean powder and 10g of starch are used to coat 2g of the probiotic microspheres to obtain a microecological composite preparation.

[0065] Comparative Example 2

[0066] The present comparative example provides a method for preparing a microecological composite preparation for lamb feeding, wherein the probiotic microspheres prepared in step 1 of Example 1 are first immersed in a 0.6wt% guar gum solution, filtered after immersion for 10s, and then immersed in a 1.5wt% modified konjac glucomannan solution prepared in Example 5, and then freeze-dried at -20°C for 30min to obtain double-layer colloid-coated probiotic microspheres, and 20g of soybean powder and 10g of starch are used to coat 2g of the probiotic microspheres to obtain a microecological composite preparation.

[0067] Prepare lamb simulated gastric juice; the preparation method of lamb simulated gastric juice is as follows: dissolve 16.4 mL of 0.1 kg / L hydrogen chloride solution in 800 mL of water, add 10 g of pepsin (1000 NFu / mg), adjust the pH to 6.5, and make up to 1000 mL; filter and sterilize with a 0.22 micron sterile filter for later use.

[0068] 70 mL of lamb simulated gastric juice was taken and divided into 7 groups on average. The probiotic composite preparations prepared in Example 1-Example 5 and Comparative Example 1-Comparative Example 2 were added to a group of lamb simulated gastric juice, respectively. After sufficient mixing, the mixture was shaken in a shaker at 37 ° C. The mixture was centrifuged and sampled at 30 min, 90 min, 180 min and 240 min, and the survival rate was calculated to obtain Table 1:

[0069] Table 1 Survival rate of microecological composite preparations prepared in Example 1-Example 5, Comparative Example 1-Comparative Example 2

[0070]

[0071]

[0072] Take out 2 g of the microecological composite preparations prepared in Examples 1 to 5 and Comparative Examples 1 to 2 respectively and put them into several sterilized bottles, that is, take out 2 g of the microecological composite preparation of each example, put 2 g of the microecological composite preparation into each sterilized bottle, seal, shield from light, and place at -18°C, 4°C, 25°C, and 37°C respectively. Take out a sterilized bottle at each time point at an interval of 15 days to determine the number of viable bacteria (CFU / g), and obtain Tables 2 to 8:

[0073] Table 2 The number of viable bacteria of the microecological composite preparation prepared in Example 1

[0074]

[0075] Table 3 The number of viable bacteria of the microecological composite preparation prepared in Example 2

[0076]

[0077] Table 4 The number of viable bacteria of the microecological composite preparation prepared in Example 3

[0078]

[0079] Table 5 The number of viable bacteria of the microecological composite preparation prepared in Example 4

[0080]

[0081] Table 6 The number of viable bacteria of the microecological composite preparation prepared in Example 5

[0082]

[0083] Table 7 The number of viable bacteria of the microecological composite preparation prepared in Comparative Example 1

[0084]

[0085] Table 8 The number of viable bacteria of the microecological composite preparation prepared in Comparative Example 2

[0086]

[0087] It can be seen from Table 2 to Table 8 that the microecological composite preparations for lamb feed prepared in Examples 1 to 5 and Comparative Examples 1 to 2 all have good storage resistance. After long-term storage, the number of viable bacteria is within a relatively high range. Among them, the storage resistance of the microecological composite preparations for lamb feed prepared in Examples 4 to 5 is higher than that in Examples 1 to 3, i.e., Comparative Examples 1 to 2.

[0088] The number of devices and processing scales described here are used to simplify the description of the present invention. Applications, modifications and variations of the present invention will be obvious to those skilled in the art.

[0089] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes, and they can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the examples shown and described herein.

Claims

1. A method for preparing a microecological composite preparation for lamb feeding, characterized in that: The following steps are involved: Step 1: activating and culturing the probiotic strains, freeze-drying to obtain probiotic powder, and preparing probiotic microspheres by mixing the probiotic powder with lactose; Step 2, dispersing the probiotic microspheres in the guar gum solution, selecting konjac glucomannan solution as the external phase, using an injection needle to absorb the guar gum solution of the dispersed oil probiotic microspheres, pumping the guar gum solution into the injection tube through a syringe pump, while the outside of the injection tube is immersed in the external phase, controlling the flow rate of the external phase and the guar gum solution, at the outlet of the injection tube, the guar gum solution and the probiotic microspheres are sheared into a spherical microsphere-double-layer gel structure by the external phase, and are collected by a collection tube and freeze-dried to obtain a microecological composite preparation inner core; Step 3: Use soybean powder and starch to coat the inner core of the microecological composite preparation to obtain the microecological composite preparation.

2. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 1, the probiotic powder includes one of Bacillus subtilis powder, Lactobacillus acidophilus powder or Lactobacillus reuteri powder.

3. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 1, the probiotic powder is a mixed powder of Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri powder, and the mass ratio of the Bacillus subtilis powder, Lactobacillus acidophilus powder and Lactobacillus reuteri is 1-3:1-3:1-3.

4. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 1, the mass ratio of probiotic powder to lactose is 2-10:1-8.

5. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 1, the specific method of preparing probiotic microspheres from probiotic powder and lactose includes: S1. Dissolve lactose and polyethylene glycol in deionized water, heat to 40-60°C and stir magnetically until completely dissolved to obtain a precursor solution; wherein the ratio of lactose, polyethylene glycol to deionized water is 1-8 g: 1-3 mL: 100-300 mL; S2, ultrasonically dispersing the probiotic powder in the precursor solution at an ultrasonic frequency of 60-80 kHz and an ultrasonic time of 2-20 min to obtain a suspension; S3. Add Span 80 and sodium alginate solution to the suspension, and use an emulsifier to emulsify to obtain an emulsion; add calcium chloride solution to the emulsion, and stir at a speed of 200-800 rpm. After the addition is completed, stop stirring, remove the upper oil layer after standing, use a centrifuge to collect the lower precipitate, and wash it with deionized water for several times to remove the residual calcium chloride and emulsifier, and obtain probiotic microspheres after freeze-drying.

6. The method for preparing the microecological composite preparation for lamb feeding according to claim 5, characterized in that: In S3, the concentration of the sodium alginate solution is 1-2wt%, the concentration of the calcium chloride solution is 10-40wt%, and the dosage ratio of lactose, Span 80, sodium alginate solution, and calcium chloride solution is 1-8g: 0.5-1g: 30-50mL: 5-10mL.

7. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 2, the flow rate of the external phase is greater than the flow rate of the guar gum solution, wherein the flow rate of the guar gum solution is 0.1-0.5 mL / min, and the flow rate of the external phase is 0.5-1.5 mL / min; the concentration of the konjac glucomannan solution is 1-2 wt%, and the concentration of the guar gum solution is 0.3-0.6 wt%.

8. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 2, the konjac glucomannan solution is replaced with a modified konjac glucomannan solution, wherein the preparation method of the modified konjac glucomannan solution comprises: A konjac glucomannan solution with a concentration of 1-2wt% and a sodium carboxymethyl cellulose solution with a concentration of 0.3-0.6wt% are prepared, the konjac glucomannan solution and the sodium carboxymethyl cellulose solution are mixed, and stirred evenly to obtain a mixed solution; the mixed solution is heated to 40-80°C, kept warm for 1-3h, cooled to room temperature, and allowed to stand for 12-36h to obtain a modified konjac glucomannan solution; wherein the volume ratio of the konjac glucomannan solution to the sodium carboxymethyl cellulose solution is 10:1-2.

9. The method for preparing the microecological composite preparation for lamb feeding according to claim 1, characterized in that: In the step 3, the mass ratio of soybean flour to starch is 2-5:

1.

10. A microecological composite preparation for lamb feeding, characterized in that: The microecological composite preparation for lamb feeding is prepared from the microecological composite preparation for lamb feeding according to any one of claims 1 to 9.

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