Natural plant extract compound formula with skin whitening enhancing effect and preparation method of natural plant extract compound formula

Abstract

The invention relates to a natural plant extract compound formula with a skin whitening enhancing effect and a preparation method of the natural plant extract compound formula, and belongs to the technical field of biology, and the natural plant extract compound formula comprises the following components: a white eggplant extract, a noctilucent flower extract, a broccoli extract, nicotinamide, resveratrol, lecithin and the like; wherein the eggplant extract is an extract which is less than 3kDa and is obtained by carrying out enzymolysis on eggplant through hemicellulase and pectinase together and then carrying out step-by-step enzymolysis on the eggplant through papain and ficin in sequence; the noctilucent noctilucent flower extract is an extract which is less than 3kDa and is obtained by sequentially carrying out step-by-step enzymolysis on the noctilucent noctilucent flower by virtue of ficin The broccoli extract is an extract which is less than 3kDa and is obtained by fermenting broccoli through streptococcus thermophilus and then carrying out enzymolysis through subtilisin. All the components are matched for use, so that the composition has outstanding anti-oxidation and whitening effects, takes effect quickly, and is safe and non-irritant.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a natural plant extract composite formula with enhanced skin whitening effect and a preparation method thereof. Background Art

[0002] The color of the skin is mainly determined by the content and distribution of melanin. Melanin is a natural pigment that is produced to protect the skin from UV damage. However, excessive melanin deposition can cause dull skin, spots and other problems, affecting appearance. Traditional skin whitening methods include the use of chemically synthesized whitening agents, but these products often have certain side effects, such as skin allergies and irritation. Natural plant extracts, as a gentle, safe and effective alternative, provide a new way to whiten the skin. Many plants are rich in ingredients with whitening effects, which work through a variety of mechanisms.

[0003] In today's society, the pursuit of beautiful and fair skin has become the desire of many people. As people pay more and more attention to health and nature, the application of natural plant extracts in the field of skin whitening has gradually gained favor. For example, licorice flavonoids in licorice extract have antioxidant and tyrosinase inhibitory effects. Tyrosinase is a key enzyme in the synthesis of melanin, and inhibiting its activity can reduce the production of melanin. Rose extract is rich in vitamin C and polyphenols, which can resist oxidation, reduce free radical damage to the skin, and inhibit the formation and transfer of melanin. Tea polyphenols can not only inhibit the activity of tyrosinase, but also promote the decomposition and metabolism of melanin by regulating cell signaling pathways.

[0004] However, natural plant extracts still face some challenges in skin whitening applications. Existing natural plant extracts for skin whitening have the following disadvantages:

[0005] 1. Quality and efficacy are affected by many factors such as plant variety and extraction process, resulting in large differences in the quality and efficacy of extracts.

[0006] 2. The transdermal absorption effect is limited. The skin's barrier function makes it difficult for some natural plant extracts to effectively penetrate the skin to reach the site of action, affecting their actual whitening effect.

[0007] 3. The effect is relatively slow. Compared with some chemically synthesized whitening ingredients, the whitening effect of natural plant extracts usually takes a long time to appear, which cannot meet consumers' expectations for rapid whitening. Summary of the invention

[0008] Aiming at the problems of large differences in quality and efficacy of existing natural plant extracts, limited transdermal absorption effect, and relatively slow effect speed. The present invention provides a natural plant extract compound formula with enhanced skin whitening effect and a preparation method thereof. The ingredients of the compound formula contain eggplant extract, night-scented lily extract, broccoli extract, niacinamide, resveratrol, lecithin, etc.; the combination of the ingredients has outstanding antioxidant and whitening effects, fast effect, and is safe and non-irritating. The specific technical scheme is as follows:

[0009] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by weight: 2 to 5 parts of Solanum melongena extract, 1 to 3 parts of Tuberose extract, 1 to 3 parts of Broccoli extract, 0.5 to 1 part of niacinamide, 0.3 to 0.8 parts of resveratrol, 0.5 to 2 parts of lecithin, 0.5 to 2 parts of carbomer, 0.01 to 0.03 parts of xanthan gum, and 50 to 60 parts of purified water;

[0010] The eggplant extract is an extract with a value below 3 kDa obtained by hydrolyzing eggplant with hemicellulase and pectinase, followed by papain and ficin; the night-scented lily extract is an extract with a value below 3 kDa obtained by hydrolyzing night-scented lily with ficin and bromelain; and the broccoli extract is an extract with a value below 3 kDa obtained by fermenting broccoli with thermophilic streptococcus and then hydrolyzing it with subtilisin.

[0011] In the above-mentioned composite formula, the preparation method of the white egg eggplant extract is as follows: take the white egg eggplant and grind it, add water 10 to 12 times the weight of the white egg eggplant, use hydrochloric acid aqueous solution to adjust the pH to 5.0 to 5.5, add hemicellulase 0.5% to 1.5% by weight of the white egg eggplant, add pectinase 0.5% to 1% by weight of the white egg eggplant, enzymolysis at 42°C to 52°C for 2h to 3h, high temperature inactivate the enzyme, after cooling to room temperature, add papain 0.5% to 1.5% by weight of the white egg eggplant, enzymolysis at 55°C to 60°C for 1.5h to 2h, high temperature inactivate the enzyme, after cooling to room temperature, use sodium hydroxide aqueous solution to adjust the pH to 6 to 7, add fig protease 0.5% to 1% by weight of the white egg eggplant, enzymolysis at 60°C to 70°C for 1h to 1.5h, high temperature inactivate the enzyme, after cooling to room temperature, use a 3kDa ultrafiltration membrane to filter, obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the white egg eggplant extract.

[0012] In the preparation method of the above-mentioned eggplant extract, the enzymatic activity of the hemicellulase is 100,000 U / g; the enzymatic activity of the pectinase is 100,000 U / g; the enzymatic activity of the papain is 400,000 U / g; and the enzymatic activity of the ficin is 100,000 U / g.

[0013] In the preparation method of the above-mentioned white egg egg solanum extract, the concentration of the hydrochloric acid aqueous solution is 0.1mol / L~0.5mol / L; the concentration of the sodium hydroxide aqueous solution is 0.1mol / L~0.5mol / L; the temperature of the high-temperature enzyme inactivation is 90℃~95℃ and the time is 15min~25min.

[0014] In the above-mentioned composite formula, the preparation method of the tuberose extract is: grind the tuberose, add water 6 to 8 times the mass of the tuberose, add 0.5% to 1% of the mass of ficin by weight of the tuberose, perform enzymolysis at 60°C to 70°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool to room temperature, add 0.5% to 1% of the mass of bromelain by weight of the tuberose, perform enzymolysis at 55°C to 60°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0015] In the preparation method of the above-mentioned night-scented lily extract, the enzymatic activity of the ficin is 100,000 U / g; the enzymatic activity of the bromelain is 200,000 U / g; the temperature of the high-temperature enzyme inactivation is 90°C to 95°C and the time is 15min to 25min.

[0016] In the above-mentioned composite formula, the preparation method of the broccoli extract is as follows: take the broccoli and crush it, add water 10 times to 12 times the mass of the broccoli, add glucose 1% to 4% of the mass of the broccoli, add casein peptone 0.5% to 2% of the mass of the broccoli, and then add thermophilic streptococci 0.5% to 1% of the mass of the broccoli, anaerobically ferment at 36°C to 38°C for 24h to 30h, sterilize at high temperature, filter with a 325-mesh to 500-mesh sieve to obtain a filtrate, adjust the pH to 8 to 9 with sodium hydroxide, add subtilisin 0.5% to 1% of the mass of the broccoli, enzymolyze at 50°C to 60°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0017] In the preparation method of the broccoli extract, the bacterial activity of the thermophilic streptococcus is 10 billion CFU / g; the temperature of the high-temperature sterilization is 121° C. to 125° C., and the time is 15 min to 20 min.

[0018] In the preparation method of the broccoli extract, the enzyme activity of the subtilisin is 20,000 U / g; the temperature of the high-temperature enzyme inactivation is 90° C. to 95° C. and the time is 15 min to 25 min.

[0019] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0020] According to the mass proportion, the purified water was heated to 75-80°C, xanthan gum was added for mixing and dissolving, the temperature was lowered to 50-60°C, lecithin and carbomer were added for even mixing, niacinamide and resveratrol were added for even mixing, the temperature was lowered to 38-45°C, and finally white eggplant extract, tuberose extract and broccoli extract were added for even mixing to obtain a compound formula.

[0021] The above-mentioned natural plant extract composite formula with enhanced skin whitening effect is used to prepare products with enhanced skin whitening effect.

[0022] The present invention provides a natural plant extract composite formula with enhanced skin whitening effect and a preparation method thereof. Compared with the prior art, the beneficial effects are as follows:

[0023] 1. The ingredients of the composite formula of the present invention include eggplant extract, tuberose extract, broccoli extract, niacinamide, resveratrol, lecithin, etc. The combination of the ingredients has outstanding antioxidant and whitening effects, is effective quickly, and is safe and non-irritating.

[0024] 2. The eggplant extract of the present invention is an extract below 3kDa obtained by hydrolyzing eggplant with hemicellulase and pectinase, and then hydrolyzing with papain and ficin step by step. The product contains various small molecule water-soluble substances and rich small molecule peptides, and has a good skin whitening effect; it helps to fight free radical damage, slow down the process of skin aging, and protect skin cells from oxidative stress, thereby maintaining the health and vitality of the skin; it has a certain moisturizing ability, helps lock skin moisture, and keeps the skin hydrated; it can reduce inflammation and irritation of the skin, making the skin more comfortable and calm; it helps to promote the metabolism of skin cells, making the skin smoother and more delicate.

[0025] 3. The tuberose extract of the present invention is an extract below 3 kDa obtained by sequential enzymatic hydrolysis of tuberose with ficin and bromelain; the product contains various small molecule water-soluble substances and rich small molecule peptides, which can help resist the damage of free radicals to the skin, slow down the speed of skin aging, and keep the skin young; reduce the inflammatory response of the skin, soothe the discomfort of the skin, and have a certain alleviating effect on sensitive skin or irritated skin; help to enhance the skin's moisturizing ability, lock in moisture, and keep the skin hydrated; can improve dull skin color by inhibiting the production of melanin or promoting the metabolism of melanin, making the skin brighter and more lustrous.

[0026] 4. The broccoli extract of the present invention is an extract below 3kDa obtained by fermenting broccoli with thermophilic streptococci and then enzymolyzing it with subtilisin. The product contains various small molecule water-soluble substances, enzymolysis and fermentation products, and is rich in various antioxidant ingredients. It can effectively fight free radicals, reduce the damage of oxidative stress to the skin, delay skin aging, and reduce the appearance of wrinkles and fine lines; help relieve skin inflammation, reduce skin sensitivity, and make the skin more stable; help strengthen the skin's natural barrier, improve the skin's resistance, and prevent the invasion of harmful substances from the outside world; lock in skin moisture, increase the skin's water content, and keep the skin hydrated and soft; and can inhibit the generation and deposition of melanin, thereby achieving a certain whitening and lightening effect of spots.

[0027] 5. The niacinamide in the ingredients can inhibit the transfer of melanin to keratinocytes and reduce pigmentation, thereby brightening the skin tone, reducing spots, and making the skin whiter and more even-toned; it can regulate sebum secretion, reduce excessive oil secretion, and at the same time help shrink pores to make the skin more delicate; enhance the skin's barrier function, improve the skin's water retention capacity, and keep the skin hydrated; reduce skin wrinkles and sagging, promote collagen production, and increase skin elasticity and firmness; help repair damaged skin barriers, enhance skin resistance, and reduce damage to the skin caused by external stimuli.

[0028] 6. Resveratrol in the ingredients can remove free radicals, protect skin cells from oxidative damage, slow down the aging process of the skin, reduce the appearance of wrinkles and sagging; inhibit the release of inflammatory factors, reduce skin inflammation, soothe the skin, and have a good improvement effect on sensitive skin or inflammatory skin; inhibit the production of melanin, reduce the formation of spots, brighten the skin tone, and make the skin whiter and more lustrous; stimulate the production of collagen and elastic fibers, enhance the elasticity and firmness of the skin, and keep the skin young. Prevent glycation reactions, reduce the formation of advanced glycation end products (AGEs), and prevent skin dullness and aging.

[0029] 7. The lecithin in the ingredients can form a protective film on the skin surface, reduce water loss, increase the moisture content of the skin, and keep the skin moisturized; it can act as an emulsifier, help to evenly mix the oil phase and water phase components, and improve the stability and texture of cosmetics; help repair damaged skin barriers and enhance the skin's resistance to external stimuli; provide nutrition to the skin, making it softer, smoother and more elastic; it can improve the permeability of the skin and promote better absorption and utilization of other skin care ingredients by the skin. DETAILED DESCRIPTION

[0030] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0031] Example 1

[0032] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by mass: 3 parts of Solanum lycopersicum extract, 2 parts of Tuberose odoratum extract, 2 parts of broccoli extract, 0.8 parts of niacinamide, 0.5 parts of resveratrol, 1.2 parts of lecithin, 1.2 parts of carbomer, 0.02 parts of xanthan gum, and 55 parts of purified water.

[0033] The preparation method of the white egg egg extract is as follows: crush the white egg egg, add water 11 times the weight of the white egg egg, adjust the pH to 5.2 with a hydrochloric acid aqueous solution with a concentration of 0.3 mol / L, add 0.8% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 0.8% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 47°C for 2.5 hours, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, add 1% of the weight of the white egg egg with an enzyme activity of 400,000 U / g papain, enzymolyze at 57°C for 1.5 hours, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, and use a concentration of 0.3 mol / L hydrochloric acid aqueous solution to adjust the pH to 5.2, add 0.8% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 47°C for 2.5 hours, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, and 1 / L sodium hydroxide aqueous solution was added to adjust the pH to 6.5, 0.8% of the mass of the white egg ...

[0034] The preparation method of the tuberose extract is as follows: crush the tuberose, add water 7 times the mass of the tuberose, add 0.8% of the mass of the tuberose with an enzyme activity of 100,000 U / g of ficin, enzymolyze at 65°C for 1 hour, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, add 0.8% of the mass of the tuberose with an enzyme activity of 200,000 U / g of bromelain, enzymolyze at 58°C for 1 hour, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0035] The preparation method of broccoli extract is as follows: crush the broccoli, add water 11 times the mass of the broccoli, add glucose 3% of the mass of the broccoli, add casein peptone 1.2% of the mass of the broccoli, and then add thermophilic streptococci with a bacterial activity of 10 billion CFU / g at 0.8% of the mass of the broccoli, anaerobically ferment at 37°C for 26 hours, sterilize at 123°C for 18 minutes, filter with a 325-mesh screen to obtain a filtrate, adjust the pH to 8.5 with sodium hydroxide, add subtilisin with an enzyme activity of 20,000 U / g at 0.8% of the mass of the broccoli, enzymolyze at 55°C for 1 hour, inactivate the enzyme at 92°C for 20 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0036] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0037] According to the mass proportion, the purified water was heated to 78°C, xanthan gum was added to mix and dissolve, the temperature was lowered to 55°C, lecithin and carbomer were added to mix evenly, niacinamide and resveratrol were added to mix evenly, the temperature was lowered to 42°C, and finally, white egg plant extract, tuberose extract and broccoli extract were added to mix evenly to obtain a compound formula.

[0038] Example 2

[0039] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by weight: 2 parts of Solanum melongena extract, 1 part of Tuberose odoriferum extract, 1 part of Broccoli extract, 0.5 parts of niacinamide, 0.3 parts of resveratrol, 0.5 parts of lecithin, 0.5 parts of carbomer, 0.01 parts of xanthan gum, and 50 parts of purified water.

[0040] The preparation method of the white egg egg extract is as follows: crush the white egg egg, add water 10 times the weight of the white egg egg, adjust the pH to 5.0 with a hydrochloric acid aqueous solution with a concentration of 0.1 mol / L, add 0.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 0.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 42°C for 2h, inactivate the enzyme at 90°C for 15min, cool to room temperature, add 0.5% of the weight of the white egg egg with an enzyme activity of 400,000 U / g papain, enzymolyze at 55°C for 1.5h, inactivate the enzyme at 90°C for 15min, cool to room temperature, and use a concentration of 0.1 mol / L hydrochloric acid aqueous solution to adjust the pH to 5.0, add 0.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 42°C for 2h, inactivate the enzyme at 90°C for 15min, cool to room temperature, 1 / L sodium hydroxide aqueous solution was added to adjust the pH to 6, 0.5% of the mass of the white egg ...

[0041] The preparation method of the tuberose extract is as follows: crush the tuberose, add water 6 times the mass of the tuberose, add 0.5% of the mass of the tuberose with an enzyme activity of 100,000 U / g of ficin, enzymolyze at 60°C for 1 hour, inactivate the enzyme at 90°C for 15 minutes, cool to room temperature, add 0.5% of the mass of the tuberose with an enzyme activity of 200,000 U / g of bromelain, enzymolyze at 55°C for 1 hour, inactivate the enzyme at 90°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0042] The preparation method of broccoli extract is as follows: crush the broccoli, add water 10 times the mass of the broccoli, add glucose 1% of the mass of the broccoli, add casein peptone 0.5% of the mass of the broccoli, and then add thermophilic streptococcus with a bacterial activity of 10 billion CFU / g at 0.5% of the mass of the broccoli, anaerobically ferment at 36°C for 24 hours, sterilize at 121°C for 15 minutes, filter with a 325-mesh screen to obtain a filtrate, adjust the pH to 8 with sodium hydroxide, add subtilisin with an enzyme activity of 20,000 U / g at 0.5% of the mass of the broccoli, enzymolyze at 50°C for 1 hour, inactivate the enzyme at 90°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0043] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0044] According to the mass proportion, the purified water was heated to 75°C, xanthan gum was added to mix and dissolve, the temperature was lowered to 50°C, lecithin and carbomer were added to mix evenly, niacinamide and resveratrol were added to mix evenly, the temperature was lowered to 38°C, and finally, Solanum oleifera extract, Tuberose extract and Broccoli extract were added to mix evenly to obtain a compound formula.

[0045] Example 3

[0046] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by mass as follows: 2 parts of Solanum melongena extract, 3 parts of Tuberose odorifera extract, 1 part of Broccoli extract, 1 part of Niacinamide, 0.3 parts of Resveratrol, 2 parts of Lecithin, 0.5 parts of Carbomer, 0.03 parts of Xanthan Gum, and 50 parts of Purified Water.

[0047] The preparation method of the white egg egg extract is as follows: crush the white egg egg, add water 12 times the weight of the white egg egg, adjust the pH to 5.5 with a hydrochloric acid aqueous solution with a concentration of 0.1 mol / L, add 0.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 1% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 42°C for 3h, inactivate the enzyme at 90°C for 25min, cool to room temperature, add 0.5% of the weight of the white egg egg with an enzyme activity of 400,000 U / g papain, enzymolyze at 60°C for 1.5h, inactivate the enzyme at 95°C for 15min, cool to room temperature, and use a concentration of 0.5 mol / L hydrochloric acid aqueous solution to adjust the pH to 5.5, add 0.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 1% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 42°C for 3h, inactivate the enzyme at 90°C for 25min, cool to room temperature, 1 / L sodium hydroxide aqueous solution was added to adjust the pH to 6, 1% of the mass of the eggplant and the enzyme activity of 100,000 U / g of fig protease was added, the eggplant was enzymolyzed at 60°C for 1.5 hours, and the enzyme was inactivated at 90°C for 25 minutes. After cooling to room temperature, the eggplant was filtered using a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, which was concentrated and freeze-dried to obtain an eggplant extract.

[0048] The preparation method of the tuberose extract is as follows: crush the tuberose, add water 6 times the mass of the tuberose, add 1% of the mass of the tuberose with an enzyme activity of 100,000 U / g of ficin, enzymolyze at 60°C for 1.5 hours, inactivate the enzyme at 90°C for 25 minutes, cool to room temperature, add 0.5% of the mass of the tuberose with an enzyme activity of 200,000 U / g of bromelain, enzymolyze at 60°C for 1 hour, inactivate the enzyme at 95°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane, obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0049] The preparation method of broccoli extract is as follows: crush the broccoli, add water 12 times the mass of the broccoli, add 1% glucose of the mass of the broccoli, add 2% casein peptone of the mass of the broccoli, and then add 0.5% thermophilic streptococcus with a bacterial activity of 10 billion CFU / g of the broccoli, anaerobically ferment at 38°C for 24 hours, sterilize at 125°C for 15 minutes, filter with a 500-mesh sieve to obtain a filtrate, adjust the pH to 8 with sodium hydroxide, add 1% subtilisin with an enzyme activity of 20,000 U / g of the broccoli, enzymolyze at 50°C for 1.5 hours, inactivate the enzyme at 90°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0050] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0051] According to the mass proportion, the purified water was heated to 75°C, xanthan gum was added to mix and dissolve, the temperature was lowered to 60°C, lecithin and carbomer were added to mix evenly, niacinamide and resveratrol were added to mix evenly, the temperature was lowered to 38°C, and finally white eggplant extract, tuberose extract and broccoli extract were added to mix evenly to obtain a compound formula.

[0052] Example 4

[0053] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by weight: 5 parts of Solanum lycopersicum extract, 1 part of Tuberose odoratum extract, 3 parts of broccoli extract, 0.5 parts of niacinamide, 0.8 parts of resveratrol, 0.5 parts of lecithin, 2 parts of carbomer, 0.01 parts of xanthan gum, and 60 parts of purified water.

[0054] The preparation method of the white egg eggplant extract is as follows: crush the white egg eggplant, add water 10 times the weight of the white egg eggplant, adjust the pH to 5.0 with a hydrochloric acid aqueous solution with a concentration of 0.5 mol / L, add 1.5% of the weight of the white egg eggplant with an enzyme activity of 100,000 U / g hemicellulase, add 0.5% of the weight of the white egg eggplant with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 52°C for 2h, inactivate the enzyme at 95°C for 15min, cool to room temperature, add 1.5% of the weight of the white egg eggplant with an enzyme activity of 400,000 U / g papain, enzymolyze at 55°C for 2h, inactivate the enzyme at 90°C for 25min, cool to room temperature, and use a concentration of 0.1 mol / L hydrochloric acid aqueous solution to adjust the pH to 5.0, add 1.5% of the weight of the white egg eggplant with an enzyme activity of 100,000 U / g hemicellulase, add 0.5% of the weight of the white egg eggplant with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 52°C for 2h, inactivate the enzyme at 95°C for 15min, cool to room temperature, 1 / L sodium hydroxide aqueous solution was added to adjust the pH to 7, 0.5% of the mass of the white eggplant with an enzyme activity of 100,000 U / g of fig protease was added, the white eggplant extract was enzymolyzed at 70°C for 1 hour, and the enzyme was inactivated at 95°C for 15 minutes. After cooling to room temperature, the extract was filtered using a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, which was concentrated and freeze-dried to obtain a white eggplant extract.

[0055] The preparation method of the tuberose extract is as follows: crush the tuberose, add water 8 times the mass of the tuberose, add 0.5% of the mass of the tuberose with an enzyme activity of 100,000 U / g of ficin, enzymolyze at 70°C for 1 hour, inactivate the enzyme at 95°C for 15 minutes, cool to room temperature, add 1% of the mass of the tuberose with an enzyme activity of 200,000 U / g of bromelain, enzymolyze at 55°C for 1.5 hours, inactivate the enzyme at 90°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane, obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0056] The preparation method of broccoli extract is as follows: crush the broccoli, add water 10 times the mass of the broccoli, add glucose 4% of the mass of the broccoli, add casein peptone 0.5% of the mass of the broccoli, and then add thermophilic streptococci with a bacterial activity of 10 billion CFU / g at 1% of the mass of the broccoli, anaerobically ferment at 36°C for 30 hours, sterilize at 121°C for 20 minutes, filter with a 325-mesh screen to obtain a filtrate, adjust the pH to 9 with sodium hydroxide, add subtilisin with an enzyme activity of 20,000 U / g at 0.5% of the mass of the broccoli, enzymolyze at 60°C for 1 hour, inactivate the enzyme at 95°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0057] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0058] According to the mass proportion, the purified water was heated to 80°C, xanthan gum was added to mix and dissolve, the temperature was lowered to 50°C, lecithin and carbomer were added to mix evenly, niacinamide and resveratrol were added to mix evenly, the temperature was lowered to 45°C, and finally white eggplant extract, tuberose extract and broccoli extract were added to mix evenly to obtain a compound formula.

[0059] Example 5

[0060] A natural plant extract compound formula with enhanced skin whitening effect, the ingredients of the compound formula are calculated by mass as follows: 5 parts of Solanum melongena extract, 3 parts of Tuberose odorifera extract, 3 parts of broccoli extract, 1 part of niacinamide, 0.8 parts of resveratrol, 2 parts of lecithin, 2 parts of carbomer, 0.03 parts of xanthan gum, and 60 parts of purified water.

[0061] The preparation method of the white egg egg extract is as follows: crush the white egg egg, add water 12 times the weight of the white egg egg, adjust the pH to 5.5 with a hydrochloric acid aqueous solution with a concentration of 0.5 mol / L, add 1.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 1% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 52°C for 3h, inactivate the enzyme at 95°C for 25min, cool to room temperature, add 1.5% of the weight of the white egg egg with an enzyme activity of 400,000 U / g papain, enzymolyze at 60°C for 2h, inactivate the enzyme at 95°C for 25min, cool to room temperature, and use a concentration of 0.5 mol / L hydrochloric acid aqueous solution to adjust the pH to 5.5, add 1.5% of the weight of the white egg egg with an enzyme activity of 100,000 U / g hemicellulase, add 1% of the weight of the white egg egg with an enzyme activity of 100,000 U / g pectinase, enzymolyze at 52°C for 3h, inactivate the enzyme at 95°C for 25min, cool to room temperature, 1 / L sodium hydroxide aqueous solution was added to adjust the pH to 7, 1% of the mass of the eggplant and the enzyme activity of 100,000 U / g of fig protease was added, the eggplant was enzymatically hydrolyzed at 70°C for 1.5 hours, and the enzyme was inactivated at 95°C for 25 minutes. After cooling to room temperature, the eggplant was filtered using a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, which was concentrated and freeze-dried to obtain an eggplant extract.

[0062] The preparation method of the tuberose extract is as follows: crush the tuberose, add water 8 times the mass of the tuberose, add 1% of the mass of the tuberose with an enzyme activity of 100,000 U / g of ficin, enzymolyze at 70°C for 1.5 hours, inactivate the enzyme at 95°C for 25 minutes, cool to room temperature, add 1% of the mass of the tuberose with an enzyme activity of 200,000 U / g of bromelain, enzymolyze at 60°C for 1.5 hours, inactivate the enzyme at 95°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane, obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain the tuberose extract.

[0063] The preparation method of broccoli extract is as follows: crush the broccoli, add water 12 times the mass of the broccoli, add 4% glucose of the broccoli, add 2% casein peptone of the broccoli, and then add 1% thermophilic streptococcus with a bacterial activity of 10 billion CFU / g of the broccoli, anaerobically ferment at 38°C for 30 hours, sterilize at 125°C for 20 minutes, filter with a 500-mesh sieve to obtain a filtrate, adjust the pH to 9 with sodium hydroxide, add 1% subtilisin with an enzyme activity of 20,000 U / g of the broccoli, enzymolyze at 60°C for 1.5 hours, inactivate the enzyme at 95°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrate, and freeze-dry to obtain a broccoli extract.

[0064] The method for preparing the above-mentioned natural plant extract composite formula having enhanced skin whitening effect comprises the following steps:

[0065] According to the mass proportion, the purified water was heated to 80°C, xanthan gum was added to mix and dissolve, the temperature was lowered to 60°C, lecithin and carbomer were added to mix evenly, niacinamide and resveratrol were added to mix evenly, the temperature was lowered to 45°C, and finally white eggplant extract, tuberose extract and broccoli extract were added to mix evenly to obtain a compound formula.

[0066] Comparative Example 1

[0067] The ingredients of the composite formula do not contain the Solanum lycopersicum extract; the mass fraction of the Solanum lycopersicum extract is replaced by purified water; other parameters and methods are the same as those in Example 1.

[0068] Comparative Example 2

[0069] In the preparation method of the eggplant extract, no ficin is used for enzymolysis; other parameters and methods are the same as those in Example 1.

[0070] Comparative Example 3

[0071] The ingredients of the composite formula do not contain tuberose extract; the mass fraction of tuberose extract is replaced by purified water; other parameters and methods are the same as those in Example 1.

[0072] Comparative Example 4

[0073] In the preparation method of the tuberose extract, bromelain is not used for enzymatic hydrolysis; other parameters and methods are the same as in Example 1.

[0074] Comparative Example 5

[0075] The ingredients of the composite formula do not contain broccoli extract; the mass fraction of broccoli extract is replaced by purified water; other parameters and methods are the same as those in Example 1.

[0076] Comparative Example 6

[0077] In the preparation method of broccoli extract, subtilisin is not used for enzymolysis; other parameters and methods are the same as in Example 1.

[0078] Comparative Example 7

[0079] The ingredients of the composite formula do not contain Solanum oleiferum extract, Solanum tuberosum extract, and Broccoli extract; the mass fractions of Solanum oleiferum extract, Solanum tuberosum extract, and Broccoli extract are replaced by purified water; other parameters and methods are the same as in Example 1.

[0080] The following tests were performed on the composite compositions of the above embodiments and comparative examples.

[0081] 1. Patch test

[0082] 24 female subjects were selected, and 0.025 g of the composition sample of each embodiment and each comparative example was placed in a spot tester chamber and applied to the inner arm of the subject. The spot tester was removed after 24 hours, and the skin condition was observed within 48 hours. All the results were negative, and the ingredients were safe and non-irritating.

[0083] 2. Hydroxyl free radical scavenging test

[0084] 10 μL of 1.25 mg / mL salicylic acid solution and 2.5 mg / mL ferrous sulfate solution were added to the ELISA plate, and then 180 μL of pure water and 40 μL of the composition (corresponding to the composition of the composite formula of each embodiment and each comparative example) were added. Finally, 10 μL of 0.03% mass concentration of H2O2 was added. After reacting for 10 minutes, the absorbance was determined using an ELISA instrument; the blank control sample was prepared by replacing 40 μL of the composition with 40 μL of pure water; 3 parallel samples were made for each type, and the hydroxyl radical scavenging rate of the composition was calculated; the test results are shown in Table 1 below.

[0085] Table 1 Hydroxyl free radical scavenging test results (average value)

[0086]

[0087] From the above results, it can be seen that the white egg ...

[0088] 3. Tyrosinase Activity Inhibition Test

[0089] A tyrosinase solution was prepared with a pH 6.8, 50 mmol / L PBS buffer solution, and a 10 g / L composition solution was prepared with a 40% volume concentration of ethanol aqueous solution; 40 μL of 0.1 mg / mL tyrosine, 80 μL of PBS buffer solution, 50 μL of the composition solution, and 50 μL of 60 U / mL tyrosinase solution were added to a 96-well plate in sequence, and incubated at 37° C. for 30 min. The plate was detected at a wavelength of 475 nm using an ELISA reader, with the PBS buffer solution as the blank group, and 3 parallel samples were made for each. The tyrosinase inhibition rate was calculated as follows:

[0090] Inhibition rate (%) = [(A1-A2)-(A3-A4)] / (A1-A2) × 100%;

[0091] Wherein, A1 is the absorbance after incubation with the blank solution, A2 is the absorbance before incubation with the blank solution, A3 is the absorbance after incubation with the combined solution, and A4 is the absorbance before incubation with the combined solution.

[0092] The test results are shown in Table 2 below.

[0093] Table 2 Tyrosinase activity inhibition test results (average value)

[0094] Sample Inhibition rate % Sample Inhibition rate % Example 1 82.7 Comparative Example 1 68.1 Example 2 76.8 Comparative Example 2 72.4 Example 3 81.4 Comparative Example 3 72.6 Example 4 84.1 Comparative Example 4 74.2 Example 5 88.3 Comparative Example 5 70.1 Comparative Example 6 73.3 Comparative Example 7 18.5

[0095] From the above results, it can be seen that the white egg ...

[0096] 4. Whitening effect trial test

[0097] 96 female subjects (25-40 years old) were selected and divided into 12 groups, 8 people in each group; the instrument detected the facial ITA° value, the larger the ITA° value, the brighter the skin color; the initial parameters were detected before using the composition; then each group of subjects used the composition sample respectively, applied the composition in the form of a mask after cleansing every night for 10 minutes for 30 days, and measured the parameters after 30 days; the ITA° value change rate was calculated, and the calculation formula was:

[0098] ITA° value change rate (%) = (ITA° value after 30 days - ITA° value before use) / ITA° value before use × 100%;

[0099] The test results are shown in Table 3 below.

[0100] Table 3I TA° value change rate (average value)

[0101]

[0102] From the above results, it can be seen that the white egg ...

Claims

1. A natural plant extract compound formula having skin whitening effect, characterized in that: The ingredients of the composite formula are calculated by weight: 2 to 5 parts of Solanum lycopersicum extract, 1 to 3 parts of Tuberose extract, 1 to 3 parts of Broccoli extract, 0.5 to 1 part of niacinamide, 0.3 to 0.8 parts of resveratrol, 0.5 to 2 parts of lecithin, 0.5 to 2 parts of carbomer, 0.01 to 0.03 parts of xanthan gum, and 50 to 60 parts of purified water; The eggplant extract is an extract with a value below 3 kDa obtained by hydrolyzing eggplant with hemicellulase and pectinase, followed by papain and ficin; the night-scented lily extract is an extract with a value below 3 kDa obtained by hydrolyzing night-scented lily with ficin and bromelain; and the broccoli extract is an extract with a value below 3 kDa obtained by fermenting broccoli with thermophilic streptococcus and then hydrolyzing it with subtilisin.

2. A composite formula of natural plant extracts with enhanced skin whitening effect according to claim 1, characterized in that: The preparation method of the eggplant extract comprises the following steps: taking the eggplant and crushing it, adding water of 10 to 12 times the mass of the eggplant, adjusting the pH value to 5.0 to 5.5 by using a hydrochloric acid aqueous solution, adding hemicellulase of 0.5 to 1.5% by mass of the eggplant, adding pectinase of 0.5 to 1% by mass of the eggplant, performing enzymolysis at 42 to 52° C. for 2 to 3 hours, inactivating the enzyme at high temperature, cooling to room temperature, adding papain of 0.5 to 1.5% by mass of the eggplant, performing enzymolysis at 55 to 60° C. for 1.5 to 2 hours, inactivating the enzyme at high temperature, cooling to room temperature, adjusting the pH value to 6 to 7 by using a sodium hydroxide aqueous solution, adding fig protease of 0.5 to 1% by mass of the eggplant, performing enzymolysis at 60 to 70° C. for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, filtering by using a 3 kDa ultrafiltration membrane to obtain a filtrate below 3 kDa, concentrating, and freeze-drying to obtain the eggplant extract.

3. A composite formula of natural plant extracts with enhanced skin whitening effect according to claim 2, characterized in that: The enzymatic activity of the hemicellulase is 100,000 U / g; the enzymatic activity of the pectinase is 100,000 U / g; the enzymatic activity of the papain is 400,000 U / g; and the enzymatic activity of the ficin is 100,000 U / g.

4. A composite formula of natural plant extracts having skin whitening effect according to claim 2, characterized in that: The concentration of the hydrochloric acid aqueous solution is 0.1 mol / L to 0.5 mol / L; the concentration of the sodium hydroxide aqueous solution is 0.1 mol / L to 0.5 mol / L; the temperature of the high temperature enzyme inactivation is 90° C. to 95° C. and the time is 15 min to 25 min.

5. A composite formula of natural plant extracts with enhanced skin whitening effect according to claim 1, characterized in that: The preparation method of the tuberose extract comprises the following steps: taking the tuberose and crushing it, adding water which is 6 to 8 times the mass of the tuberose, adding 0.5% to 1% of the mass of ficin by the mass of the tuberose, performing enzymolysis at 60 to 70°C for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, adding 0.5% to 1% of the mass of bromelain by the mass of the tuberose, performing enzymolysis at 55 to 60°C for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, filtering with a 3 kDa ultrafiltration membrane to obtain a filtrate below 3 kDa, concentrating, and freeze-drying to obtain the tuberose extract.

6. A natural plant extract composite formula with enhanced skin whitening effect according to claim 5, characterized in that: The enzymatic activity of the ficin is 100,000 U / g; the enzymatic activity of the bromelain is 200,000 U / g; the temperature of the high-temperature enzyme inactivation is 90° C. to 95° C. and the time is 15 min to 25 min.

7. A composite formula of natural plant extracts with enhanced skin whitening effect according to claim 1, characterized in that: The preparation method of the broccoli extract comprises the following steps: taking broccoli and crushing it, adding water of 10 to 12 times the mass of the broccoli, adding glucose of 1% to 4% the mass of the broccoli, adding casein peptone of 0.5% to 2% the mass of the broccoli, and then adding thermophilic streptococci of 0.5% to 1% the mass of the broccoli, performing anaerobically fermentation at 36 to 38°C for 24 to 30 hours, sterilizing at high temperature, filtering through a 325-mesh to 500-mesh sieve to obtain a filtrate, adjusting the pH value to 8 to 9 through sodium hydroxide, adding subtilisin of 0.5% to 1% the mass of the broccoli, performing enzymolysis at 50 to 60°C for 1 hour to 1.5 hours, killing the enzyme at high temperature, cooling to room temperature, filtering through a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, concentrating, and freeze-drying to obtain the broccoli extract.

8. A natural plant extract composite formula with enhanced skin whitening effect according to claim 7, characterized in that: The bacterial activity of the thermophilic streptococcus is 10 billion CFU / g; the temperature of the high-temperature sterilization is 121° C. to 125° C., and the time is 15 min to 20 min.

9. A natural plant extract composite formula with enhanced skin whitening effect according to claim 7, characterized in that: The enzyme activity of the subtilisin is 20,000 U / g; the temperature of the high-temperature enzyme inactivation is 90° C. to 95° C. and the time is 15 min to 25 min.

10. A method for preparing a natural plant extract compound formula with enhanced skin whitening effect, used for preparing a natural plant extract compound formula with enhanced skin whitening effect as claimed in claim 1, characterized in that: The preparation method comprises the following steps: According to the mass proportion, the purified water was heated to 75-80°C, xanthan gum was added for mixing and dissolving, the temperature was lowered to 50-60°C, lecithin and carbomer were added for even mixing, niacinamide and resveratrol were added for even mixing, the temperature was lowered to 38-45°C, and finally white eggplant extract, tuberose extract and broccoli extract were added for even mixing to obtain a compound formula.

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