Anti-aging composition containing sheep placenta and application and cosmetics thereof

Abstract

The invention belongs to the technical field of cosmetic production, and discloses an anti-aging composition containing sheep placenta extract, and the anti-aging composition is prepared from, by mass, 15-25 parts of hydrolyzed sheep placenta extract, 8-12 parts of tremella fuciformis extract, 1-3 parts of allantoin and 1-3 parts of amino acid.Through matching of the raw materials, the amino acid is beneficial to enhancing the barrier function of skin, the skin can be effectively protected, and the anti-aging effect is good. The composition has the advantages that by combining the moisturizing characteristic of the allantoin, the skin health can be maintained, the external invasion can be prevented, the balance state of the skin can be synergistically promoted through the protection provided by the hydrolyzed sheep placenta extract and the tremella fuciformis extract so as to resist aging, and in addition, the invention also discloses the application of the composition and a cosmetic.

Description

Technical Field

[0001] The present application relates to the technical field of daily chemical product production, and in particular to an anti-aging composition containing sheep placenta, and its application and cosmetics. Background Art

[0002] Skin aging is a natural physiological process, but many factors in modern life accelerate this process, especially the effects of oxidation, dryness and free radicals. They not only directly affect the appearance of the skin, but also cause a series of changes at the cellular level, leading to accelerated skin aging.

[0003] Oxidation is one of the important factors of skin aging. Free radicals are unstable molecules that are produced due to the external environment (such as ultraviolet rays, air pollution, smoking, etc.) or metabolism in the body. Free radicals can cause damage to cells and tissues, especially in the skin. Oxidative stress leads to the degradation of collagen and elastin, accelerating the aging process of the skin. At the same time, free radicals attack the membranes, proteins and DNA of skin cells, causing damage to their structure and function. Damaged cells not only cannot work properly, but may also trigger an inflammatory response, further damaging surrounding cells, and as free radicals increase, collagen in the skin gradually decreases, causing the skin to lose its support and elasticity, forming wrinkles.

[0004] Dryness is another cause of skin aging, especially in winter or dry environments, which increases the loss of skin moisture, causing the stratum corneum to thicken, slow cell renewal, and appear rough and dull. As we age, the skin's oil and moisture secretion decreases, and the skin's barrier function declines, leading to moisture loss. Dehydrated skin is prone to dryness, fine lines, and sagging. In addition, dryness will weaken the skin's defense function, making it more susceptible to external irritation and infection, forming a vicious cycle.

[0005] In order to combat the effects of oxidation, dryness and free radicals, modern science has developed a variety of anti-aging methods that not only improve the appearance of the skin, but also enhance the overall health of the skin. For example, the use of antioxidants such as vitamin C, vitamin E, and niacinamide can effectively neutralize free radicals and reduce oxidative damage. Regular use of skin care products containing antioxidants can significantly slow down skin aging;

[0006] Maintaining proper moisture levels can help relieve dry skin and reduce the appearance of fine lines. Moisturizing also aids cell metabolism and promotes the skin's natural renewal process.

[0007] Chinese patent application 202110264479.X discloses an anti-aging and skin rejuvenation essence and a preparation method thereof, which are used to promote skin metabolism and delay skin aging. The anti-aging and skin rejuvenation essence is compounded from the following raw materials: moisturizing ingredients; hydrolyzed placenta extract, carnosine, glutathione, superoxide dismutase, tocopherol, ascorbic acid, α-arbutin, kojic acid dipalmitate; rhodiola rosea extract, ginkgo leaf extract, grape skin extract, scutellaria baicalensis extract, tremella extract; preservative system; chelating agent; the balance is water; the scheme is based on the biomimetic principle of bioengineering, and compounds active substances such as cell nutrients, enzyme substances, and natural plant extracts to achieve a synergistic effect, taking into account safety and functionality, delaying skin aging from the mechanism of skin aging, promoting regeneration, and scavenging free radicals, and has good moisturizing and anti-wrinkle and anti-aging effects;

[0008] Further observation of the scheme shows that the hydrolyzed placenta extract used is used to promote collagen and elastin synthesis, Tremella fuciformis is used to prevent cell aging, and allantoin is used to moisturize the skin to keep it hydrated. In addition, the scheme does not disclose the use of amino acids.

[0009] Chinese patent application 202211582237.6 discloses a natural anti-wrinkle and firming essence composed of the following parts by weight: 10-18 parts of Tremella extract, 10-18 parts of ginseng fruit extract, 2-8 parts of plant placenta, 2-8 parts of soybean extract, 10-18 parts of aloe extract, 0.5-4 parts of Zanthoxylum extract, 0.5-4 parts of pepper extract, 20-31 parts of traditional Chinese medicine anti-aging extract, 4-14 parts of flavoring extract, 2-8 parts of antibacterial additive, 5-12 parts of retinol, 5-12 parts of hydroxy acid, 10-18 parts of pentapeptide, 10-18 parts of hexapeptide, 2-7 parts of gel quick-setting agent, 20-31 parts of 75% alcohol, and 5-12 parts of glycerin.

[0010] This solution contains a large amount of plant ingredients, and uses perfume extract to increase the fragrance of the essence. Flowers and fruit peels are used as fragrance enhancers, and the fragrance is light and lasting. The essence contains a variety of materials that can resist wrinkles and improve firmness, which improves the effectiveness of the essence. The Chinese herbal ingredients are anti-aging and can repair the skin.

[0011] However, it can be seen from the above scheme that the placenta used is plant-based placenta. At the same time, the scheme also does not disclose the use of amino acids.

[0012] The problem to be solved in this scheme is: how to develop a composition that is different from the prior art and has good anti-aging ability. Summary of the invention

[0013] The purpose of the present application is to provide an anti-aging composition, which enhances the free radical scavenging ability and elastase inhibition ability of the sample through the combined action of hydrolyzed sheep placenta extract, tremella extract, allantoin and amino acids.

[0014] To achieve the above-mentioned purpose, the present application discloses an anti-aging composition containing sheep placenta, which comprises the following components by weight:

[0015]

[0016] Among the above components, hydrolyzed sheep placenta extract is rich in peptides and sugars, which can effectively lock in skin moisture and improve skin hydration. The growth factors contained therein can promote cell regeneration and repair, help slow down skin aging, and improve the appearance of fine lines and wrinkles. In addition, hydrolyzed sheep placenta extract helps improve local blood circulation in the skin, making the skin more rosy and shiny, and, to a certain extent, its ingredients help reduce skin inflammation, relieve skin irritation and allergic reactions, and improve skin tolerance.

[0017] Tremella extract contains high levels of polysaccharides, which can form a protective film on the skin surface, lock in moisture, increase the moisture content of the skin, and provide deep moisturizing. At the same time, it is rich in natural antioxidant ingredients that can resist free radicals, and has a positive effect on alleviating skin aging and reducing the formation of fine lines and wrinkles. Tremella extract can also enhance the skin's barrier function, making the skin stronger and more resistant to external environmental stimulation.

[0018] Allantoin is an effective moisturizer that can attract and retain moisture, help improve skin hydration, and is suitable for dry skin. Allantoin also has a slight exfoliating effect, which can help remove dead skin cells, promote skin renewal, and make the skin smoother and more delicate. In addition, allantoin can enhance the skin's barrier function, help resist external stimuli, and keep the skin healthy.

[0019] Amino acids are the main component of natural moisturizing factor (NMF), which can help the skin retain moisture, improve skin hydration, and make the skin soft and smooth; amino acids are the basic units of protein, which can promote the repair and regeneration of skin cells and help improve the overall condition of the skin; in addition, amino acids also have antioxidant properties, which can resist damage from free radicals, help slow down the skin's aging process, and reduce the appearance of fine lines and wrinkles; amino acids can also enhance the skin's barrier function, help resist external stimuli and pollution, and protect skin health.

[0020] Preferably, the extraction method of the hydrolyzed sheep placenta extract is: taking a fresh sheep placenta sample, washing and crushing it, then mixing it with an aqueous solution of an inorganic salt, heating, keeping warm, and enzymolysis, and taking the supernatant after enzymolysis to obtain the hydrolyzed sheep placenta extract;

[0021] The inorganic salt is a strong acid and weak base salt;

[0022] In the enzymatic hydrolysis process, the enzyme used is at least one of papain, acid protease, neutral protease and bromelain.

[0023] Preferably, the inorganic salt is selected from at least one of copper sulfate, ammonium sulfate, calcium chloride and magnesium chloride;

[0024] The pH value of the aqueous solution of the inorganic salt is 4-6.

[0025] Preferably, the Tremella fuciformis extract is extracted from the fruiting body part of Tremella fuciformis.

[0026] Preferably, the amino acid is a basic amino acid, and the basic amino acid is selected from at least one of lysine, histidine and arginine.

[0027] In addition, the present application also discloses the use of the anti-aging composition containing sheep placenta as described above in preparing cosmetics.

[0028] In addition, the present application also discloses a cosmetic containing 5 to 30 wt % of the above anti-aging composition containing sheep placenta.

[0029] Preferably, it also contains skin conditioners, moisturizers, antioxidants and chelating agents;

[0030] The skin conditioning agent is selected from at least one of hydroxyphenylpropionamide benzoic acid, ascorbyl palmitate, acetyl hexapeptide-8, hydroxyethylpiperazine ethane sulfonic acid, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate;

[0031] The moisturizing agent is selected from at least one of butylene glycol, glycerin, 1,2-hexanediol, sodium hyaluronate, and hydrolyzed sodium hyaluronate;

[0032] The antioxidant is hydroxyacetophenone;

[0033] The chelating agent is disodium ethylenediaminetetraacetate.

[0034] Preferably, the skin conditioning agent comprises at least acetyl hexapeptide-8.

[0035] Preferably, the sodium hyaluronate in the skin conditioning agent is sodium hyaluronate with a molecular weight of less than 10 KDa;

[0036] The sodium hyaluronate in the moisturizing agent is sodium hyaluronate with a molecular weight greater than or equal to 1000 KDa.

[0037] The beneficial effects of this application are:

[0038] The hydrolyzed sheep placenta extract in the present application is rich in peptides and sugars, which can effectively lock in skin moisture and improve skin hydration. The growth factors contained therein can promote cell regeneration and repair, help slow down skin aging, and improve the appearance of fine lines and wrinkles. In addition, the hydrolyzed sheep placenta extract helps improve local blood circulation in the skin, making the skin more rosy and shiny.

[0039] Tremella extract contains high levels of polysaccharides, which can form a protective film on the skin surface, lock in moisture, increase the moisture content of the skin, and provide deep moisturizing. At the same time, the rich natural antioxidant ingredients can resist free radicals, which has a positive effect on alleviating skin aging and reducing the formation of fine lines and wrinkles. In addition, Tremella extract can promote skin metabolism, help improve uneven skin tone, and enhance the natural luster of the skin. Tremella extract can also enhance the skin's barrier function, making the skin stronger and more resistant to external environmental stimulation.

[0040] Allantoin is an effective moisturizer that can attract and retain moisture, help improve skin hydration, and is suitable for dry skin. Allantoin also has a slight exfoliating effect, which can help remove dead skin cells, promote skin renewal, and make the skin smoother and more delicate. In addition, allantoin can enhance the skin's barrier function, help resist external stimuli, and keep the skin healthy.

[0041] Amino acids are the main components of natural moisturizing factor (NMF), which can help the skin retain moisture, improve skin hydration, and make the skin soft and smooth; amino acids are the basic units of protein, which can promote the repair and regeneration of skin cells and help improve the overall condition of the skin; in addition, amino acids also have antioxidant properties, which can resist damage from free radicals, help slow down the skin aging process, and reduce the appearance of fine lines and wrinkles; amino acids can also enhance the skin's barrier function, help resist external irritation and pollution, and protect skin health;

[0042] In addition, through the combination of the above-mentioned raw materials, the amino acids in this application help to enhance the barrier function of the skin. Combined with the moisturizing properties of allantoin, it helps to maintain skin health and prevent external damage. Further, through the protection provided by hydrolyzed sheep placenta extract and tremella extract, it synergistically promotes the balance of the skin and thus resists aging. DETAILED DESCRIPTION

[0043] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0044] Before presenting the embodiments and comparative examples, the raw materials involved in the embodiments and comparative examples are described as follows, as shown in Table 1:

[0045] Table 1

[0046]

[0047] It should be noted that, among the above raw materials, the molecular weight of sodium hyaluronate (HA-TLM 3-5S) is low molecular weight sodium hyaluronate of "37KDa-56KDa";

[0048] The molecular weight of sodium hyaluronate (HA-THM) is high molecular weight sodium hyaluronate of "greater than or equal to 1.8 MDa".

[0049] Extraction method of hydrolyzed sheep placenta extract:

[0050] Take a fresh sheep placenta sample and rinse it with normal saline for 6 times, then cut off the umbilical cord and fascia with sterilized scissors, and then rinse it with deionized water for 6 times;

[0051] The washed sheep placenta sample was broken into small pieces of 1 cm × 1 cm, 150 g of which was taken and soaked in 300 ml of an aqueous solution of magnesium sulfate (the concentration of magnesium sulfate was 0.12 mol / L), then heated to 70 ° C and kept warm for 30 min;

[0052] After the heat preservation, papain was added and enzymolyzed for 6 hours (papain was added at a mass ratio of 0.5:10 to the sheep placenta sample), the enzyme was inactivated and allowed to stand for 12 hours, and the supernatant was taken to obtain a hydrolyzed sheep placenta extract. (Unless otherwise specified, the extraction method of the hydrolyzed sheep placenta extract used in the examples and comparative examples all adopted the above method)

[0053] It should be noted that the Tremella extract was purchased from Shanghai Huiwen Biotechnology Co., Ltd. and extracted from the fruiting body of Tremella, and the content of the effective substance was 1%;

[0054] The complete components of the Tremella extract are: 1% Tremella fruiting body extract by mass, 1.5% butylene glycol by mass, 0.5% phenoxyethanol by mass, 0.5% p-hydroxyacetophenone by mass, and 96.5% water by mass. (Unless otherwise specified, the Tremella extracts used in the examples and comparative examples are all the Tremella extracts mentioned above)

[0055] The preparation method of the anti-aging composition containing sheep placenta extract is as follows: hydrolyzed sheep placenta extract, tremella extract, allantoin and amino acids are weighed according to mass fractions, mixed and homogenized to obtain the anti-aging composition containing sheep placenta extract; for the specific addition amounts of hydrolyzed sheep placenta extract, tremella extract, allantoin and amino acids and the specific selection of amino acids, see the specific description of each embodiment.

[0056] Examples 1-4

[0057] An anti-aging composition containing sheep placenta, the formula of which is shown in Table 2:

[0058] Table 2

[0059]

[0060] It should be noted that the amino acids in Examples 1-4 are all lysine.

[0061] Example 5

[0062] The method is substantially the same as Example 1, except that lysine is replaced by arginine of the same mass.

[0063] Example 6

[0064] The method is substantially the same as Example 1, except that the same mass of acidic amino acid (specifically glutamic acid) is used to replace lysine.

[0065] Example 7

[0066] It is basically the same as Example 1, except that a neutral amino acid (specifically glycine) of the same mass is used to replace lysine.

[0067] Example 8

[0068] The method is substantially the same as Example 1, except that during the extraction process of the hydrolyzed sheep placenta extract, bromelain of the same mass is used to replace papain.

[0069] Example 9

[0070] The method is basically the same as Example 1, except that in the extraction process of hydrolyzing the sheep placenta extract, the same mass of neutral protease is used to replace papain.

[0071] Example 10

[0072] The method is basically the same as Example 1, except that, during the extraction process of the hydrolyzed sheep placenta extract, a mixture of neutral protease and bromelain of the same mass is used to replace papain, and the mass ratio of neutral protease to bromelain in the mixture is 1:1.

[0073] Embodiment 11

[0074] The method is basically the same as Example 1, except that the Tremella fuciformis extract in Example 1 is replaced by a commercially available Tremella fuciformis extract (the commercially available Tremella fuciformis extract is diluted with pure water to the same mass fraction as the Tremella fuciformis extract in Example 1);

[0075] The commercially available Tremella fuciformis extract is extracted from the whole Tremella fuciformis plant (including the fruiting body part and the root of Tremella fuciformis).

[0076] Comparative Examples 1-4

[0077] A composition, the formula is shown in Table 3:

[0078] Table 3

[0079]

[0080]

[0081] Comparative Examples 5-10

[0082] A composition, the formula is shown in Table 3:

[0083] Table 4

[0084]

[0085] Performance Test:

[0086] 1.DPPH free radical scavenging rate test

[0087] 1.1 Instruments and Equipment

[0088] BSA224S analytical balance, L6s UV spectrophotometer

[0089] 1.2 Reagents

[0090] DPPH (1,1-diphenyl-2-picrylhydrazyl), 98%

[0091] 1.3 Test methods

[0092] (1) Treatment of control and test samples

[0093] Sample group: The compositions prepared in the examples and comparative examples were diluted with pure water to a mass fraction of 3.3%;

[0094] Negative control: pure water.

[0095] (2) Test operation steps

[0096] Set up a sample group and a negative control group. Set up 3 parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0097] (3) The calculation formula is shown in Formula 1:

[0098]

[0099] Where: T—absorbance of sample tube, i.e. absorbance of solution after sample reacts with DPPH;

[0100] T0—sample background absorbance;

[0101] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution when no sample is added;

[0102] C0—Solvent background absorbance.

[0103] (4) Data analysis

[0104] The statistical analysis software was SPSS, and the comparison between the DPPH free radical scavenging rate of the test sample and the negative control was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0105] The test results are shown in Table 5:

[0106] Table 5

[0107]

[0108] Result analysis:

[0109] 1. Referring to Table 5, it can be seen from Examples 1-4 that when the amount of each raw material added in the composition is slightly adjusted, the DPPH radical scavenging rate of Examples 1-4 fluctuates. Further, in combination with Table 2, it can be seen that as the total mass of the raw materials in Examples 1-4 increases or decreases, the DPPH radical scavenging rate increases or decreases slightly. It can be seen that the total mass fraction of the composition has a certain influence on its DPPH radical scavenging rate;

[0110] 2. It can be seen from Examples 1 and 5-7 that when lysine is replaced by arginine, which is also a basic amino acid, the DPPH free radical scavenging rate of Example 5 has a significant upward trend relative to Example 1. The reason for this phenomenon may be that arginine is a precursor for the synthesis of nitric oxide, and NO has an antioxidant effect and can help scavenge free radicals in the body. By increasing the generation of NO, arginine may enhance the antioxidant effect of hydrolyzed sheep placenta extract, allantoin and tremella extract, thereby producing a more excellent synergistic effect to enhance the DPPH free radical scavenging ability of the composition;

[0111] Further observation of Examples 6-7 shows that when acidic amino acids or neutral amino acids are used to replace the basic amino acid (lysine) in Example 1, the DPPH free radical scavenging rate of Examples 6-7 has a significant downward trend. The reason may be that acidic amino acids and neutral amino acids are different from basic amino acids in chemical properties and physiological functions. Their charge, polarity and structural characteristics may not be conducive to effective combination with the active ingredients in the hydrolyzed sheep placenta extract and Tremella fuciformis extract, thereby reducing the synergistic effect;

[0112] 3. It can be seen from Examples 1 and 8-10 that when the hydrolyzed sheep placenta is enzymatically hydrolyzed using a mixed enzyme of neutral protease and bromelain, the composition prepared from the extract has a more excellent DPPH free radical scavenging ability. At the same time, it can be seen from Examples 8-9 that when bromelain and neutral protease are used for enzymatic hydrolysis, the DPPH free radical scavenging rates are 38.306% and 38.613%, respectively, while when bromelain and neutral protease are used at the same time in Example 10, the DPPH free radical scavenging rate reaches 41.067%. It can be seen that the free radical scavenging ability of the composition prepared in Example 10 exceeds that of any of Examples 8 and 9. It can be seen that bromelain and neutral protease produce a synergistic effect so that the content of substances with free radical scavenging ability in the hydrolyzed sheep placenta extract is increased;

[0113] 4. It can be seen from Examples 1 and 11 that when the Tremella extract obtained by extracting the whole Tremella plant is used to replace the Tremella extract obtained by extracting the Tremella fruiting body, the free radical scavenging ability of Example 11 shows a certain degree of downward trend relative to Example 1. It can be seen that the Tremella fruiting body has more active substances with free radical scavenging ability;

[0114] 5. It can be seen from Example 1 and Comparative Examples 1-4 that when the composition lacks any one of the hydrolyzed sheep placenta extract, Tremella fuciformis extract, allantoin, and amino acids, the free radical scavenging ability of the composition obtained in Comparative Examples 1-4 has a decreasing trend to varying degrees relative to Example 1. It can be seen that the above four raw materials are indispensable;

[0115] Further observing comparative examples 5-10, when the hydrolyzed sheep placenta extract was replaced with plant placenta, the Tremella extract was replaced with mannose and fucose rich in Tremella extract, and the amino acid was replaced with pentapeptide or glutathione composed of multiple amino acid units, the free radical scavenging ability of comparative examples 5-10 all declined. It can be seen that the hydrolyzed sheep placenta extract, Tremella extract, allantoin and amino acids in the present application are difficult to be replaced.

[0116] 2. Elastase inhibition test

[0117] 2.1 Instruments and Equipment

[0118] BSA224S analytical balance, RT-6100 ELISA analyzer;

[0119] 2.2 Reagents

[0120] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0121] 2.3 Test methods

[0122] (1) Treatment of control and test samples

[0123] Sample group: The compositions prepared in the examples and comparative examples were diluted with pure water to a mass fraction of 3.3%;

[0124] Negative control: pure water.

[0125] (2) Test operation steps

[0126] Set up a sample group and a negative control group, and set up 3 parallels for each group. Add the corresponding reagent solution into a 96-well plate, shake it gently, incubate it at 25°C for 15 minutes, place it in an enzyme reader, and measure the absorbance at 410nm.

[0127] (3) The calculation formula is shown in Formula 2:

[0128]

[0129] Where: A—is the absorbance of the reaction solution without sample;

[0130] B—is the absorbance of the reaction solution without sample and enzyme;

[0131] C—is the absorbance of the reaction solution containing sample and enzyme;

[0132] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0133] (4) Data analysis

[0134] The statistical analysis software was SPSS, and the comparison between the elastase inhibition rate of the test sample and the negative control was performed using the independent sample t test. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0135] The test results are shown in Table 6:

[0136] Table 6

[0137]

[0138] Result analysis:

[0139] 1. Referring to Table 6, it can be seen from Examples 1-4 that when the amount of each raw material added in the composition is slightly adjusted, the elastase inhibition rate of Examples 1-4 fluctuates. Further combined with Table 2, it can be seen that as the total mass of the raw materials in Examples 1-4 increases or decreases, the elastase inhibition rate increases or decreases slightly. It can be seen that the total mass fraction of the composition has a certain influence on its elastase inhibition rate;

[0140] 2. It can be seen from Examples 1 and 5-7 that when lysine is replaced by arginine, which is also a basic amino acid, the elastase inhibition rate of Example 5 has a significant improvement trend relative to Example 1. The reason for this phenomenon may be that arginine has a long side chain and a positively charged amino group, which can interact strongly with other molecules (such as enzymes and growth factors) in the body. This structure enables arginine to more effectively inhibit the activity of elastase when it binds to it, thereby improving the inhibitory effect on it;

[0141] Further observation of Examples 6-7 shows that when acidic amino acids or neutral amino acids are used to replace the basic amino acid (lysine) in Example 1, the elastase inhibition rate of Examples 6-7 has a significant downward trend. The reason may be that acidic amino acids and neutral amino acids are different from basic amino acids in chemical properties and physiological functions. Their charge, polarity and structural characteristics may not be conducive to effective combination with the active ingredients in the hydrolyzed sheep placenta extract and Tremella fuciformis extract, thereby reducing the synergistic effect;

[0142] 3. It can be seen from Examples 1 and 8-10 that when the hydrolyzed sheep placenta is enzymatically hydrolyzed using a mixed enzyme of neutral protease and bromelain, the composition prepared from the extract has a more excellent elastase ability. At the same time, it can be seen from Examples 8-9 that when bromelain and neutral protease are used for enzymatic hydrolysis, the elastase inhibition rates are 33.874% and 34.785% respectively, while when bromelain and neutral protease are used at the same time in Example 10, the elastase inhibition rate reaches 37.094%. It can be seen that the elastase inhibition ability of the composition prepared in Example 10 exceeds that of any of Examples 8 and 9. It can be seen that bromelain and neutral protease produce a synergistic effect so that the content of substances with elastase inhibition ability in the hydrolyzed sheep placenta extract is increased;

[0143] 4. It can be seen from Examples 1 and 11 that when the Tremella extract obtained by extracting the whole Tremella plant is used to replace the Tremella extract obtained by extracting Tremella fruiting bodies, the elastase inhibition ability of Example 11 shows a certain degree of downward trend relative to Example 1. It can be seen that Tremella fruiting bodies have more active substances with elastase inhibition ability;

[0144] 5. It can be seen from Example 1 and Comparative Examples 1-4 that when the composition lacks any one of the hydrolyzed sheep placenta extract, Tremella fuciformis extract, allantoin, and amino acids, the elastase inhibition ability of the compositions prepared in Comparative Examples 1-4 has a decreasing trend to varying degrees relative to that of Example 1. It can be seen that the above four raw materials are indispensable;

[0145] Further observation of Comparative Examples 5-10, when the hydrolyzed sheep placenta extract was replaced with plant placenta, the Tremella extract was replaced with mannose and fucose rich in Tremella extract, and the amino acid was replaced with pentapeptide or glutathione composed of multiple amino acid units, the elastase inhibition ability of Comparative Examples 5-10 all declined. It can be seen that the hydrolyzed sheep placenta extract, Tremella extract, allantoin and amino acids in the present application are difficult to be replaced.

[0146] Application Example 1

[0147] The composition prepared in Example 1 is mixed with a skin conditioner, a moisturizer, an antioxidant and a chelating agent and uniformly dispersed to obtain an essence of an anti-aging composition containing sheep placenta;

[0148] More specifically, the skin conditioning agent is acetyl hexapeptide-8 (BMS-acetyl hexapeptide-8 (L)), and the mass fraction in the essence of the anti-aging composition containing sheep placenta is 2.5%;

[0149] The moisturizer is a mixture of sodium hyaluronate (HA-TLM 3-5S), butylene glycol and glycerol. In the essence of the anti-aging composition containing sheep placenta, the mass fraction of sodium hyaluronate (HA-TLM 3-5S) is 0.6%;

[0150] The mass fraction of butanediol is 5%;

[0151] The mass fraction of glycerol is 4%;

[0152] The antioxidant is p-hydroxyacetophenone, and its mass fraction in the essence of the anti-aging composition containing sheep placenta is 0.5%;

[0153] The chelating agent is EDTA-disodium, and the mass fraction of the essence in the anti-aging composition containing sheep placenta is 0.03%;

[0154] The composition has a mass fraction of 3.3% in the essence of the anti-aging composition containing sheep placenta, and water is used as a solvent to supplement the overall mass fraction to 100%.

[0155] Application Example 2

[0156] The method is basically the same as Application Example 1, except that no skin conditioner is added to the essence of the anti-aging composition containing sheep placenta, and water is used to supplement the overall mass fraction to 100%.

[0157] Application Example 3

[0158] The method is basically the same as Application Example 1, except that no moisturizer is added to the essence of the anti-aging composition containing sheep placenta, and water is used to supplement the overall mass fraction to 100%.

[0159] Application Example 4

[0160] The method is basically the same as Application Example 1, except that no antioxidant is added to the essence of the anti-aging composition containing sheep placenta, and water is used to supplement the overall mass fraction to 100%.

[0161] Application Example 5

[0162] The method is basically the same as Application Example 1, except that the moisturizer is a mixture of butylene glycol and glycerol, and the mass fraction of butylene glycol is 5.3%;

[0163] The mass fraction of glycerol is 4.3%.

[0164] Application Example 6

[0165] The invention is basically the same as Application Example 1, except that the skin conditioning agent is a mixture of acetyl hexapeptide-8 (BMS-acetyl hexapeptide-8 (L)) and sodium hyaluronate (HA-THM), and the mass fraction of acetyl hexapeptide-8 (BMS-acetyl hexapeptide-8 (L)) in the essence of the anti-aging composition containing sheep placenta is 2.25%;

[0166] The mass fraction of sodium hyaluronate (HA-THM) in the essence of the anti-aging composition containing sheep placenta is 0.25%.

[0167] Application Example 7

[0168] It is basically the same as Application Example 1, except that the skin conditioning agent is sodium hyaluronate (HA-THM), and the mass fraction of sodium hyaluronate (HA-THM) in the essence of the anti-aging composition containing sheep placenta is 2.5%.

[0169] 3.DPPH free radical scavenging rate test

[0170] 3.1 Instruments and Equipment

[0171] BSA224S analytical balance, L6s UV spectrophotometer

[0172] 3.2 Reagents

[0173] DPPH (1,1-diphenyl-2-picrylhydrazyl), 98%

[0174] 3.3 Test methods

[0175] (1) Treatment of control and test samples

[0176] Sample group: products prepared in application examples 1-7;

[0177] Negative control: pure water.

[0178] (2) Test operation steps

[0179] Set up a sample group and a negative control group. Set up 3 parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0180] (3) The calculation formula is shown in Formula 3:

[0181]

[0182] Where: T—absorbance of sample tube, i.e. absorbance of solution after sample reacts with DPPH;

[0183] T0—sample background absorbance;

[0184] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution when no sample is added;

[0185] C0—Solvent background absorbance.

[0186] (4) Data analysis

[0187] The statistical analysis software was SPSS, and the comparison between the DPPH free radical scavenging rate of the test sample and the negative control was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0188] The test results are shown in Table 7:

[0189] Table 7

[0190]

[0191] 4. Elastase inhibition test

[0192] 4.1 Instruments and Equipment

[0193] BSA224S analytical balance, RT-6100 ELISA analyzer;

[0194] 4.2 Reagents

[0195] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0196] 4.3 Test methods

[0197] (1) Treatment of control and test samples

[0198] Sample group: products prepared in application examples 1-7;

[0199] Negative control group: pure water;

[0200] (2) Test operation steps

[0201] Set up a sample group and a negative control group, and set up 3 parallels for each group. Add the corresponding reagent solution into a 96-well plate, shake it gently, incubate it at 25°C for 15 minutes, place it in an enzyme reader, and measure the absorbance at 410nm.

[0202] (3) The calculation formula is shown in Formula 2:

[0203]

[0204] Where: A—is the absorbance of the reaction solution without sample;

[0205] B—is the absorbance of the reaction solution without sample and enzyme;

[0206] C—is the absorbance of the reaction solution containing sample and enzyme;

[0207] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0208] (4) Data analysis

[0209] The statistical analysis software was SPSS, and the comparison between the elastase inhibition rate of the test sample and the negative control was performed using the independent sample t test. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0210] The test results are shown in Table 8:

[0211] Table 8

[0212]

[0213] Result analysis:

[0214] 1. As can be seen from Tables 7-8, when Example 1 is used with a skin conditioner, a moisturizer, an antioxidant and a chelating agent to prepare a product, the free radical scavenging ability and elastase inhibition ability of the product are further improved compared to using the composition alone;

[0215] 2. Further observation of Application Examples 2-4 shows that when any one of the skin conditioner, moisturizer and antioxidant is missing from the product, the free radical scavenging ability and elastase inhibition ability of Application Examples 2-4 are both reduced relative to Application Example 1;

[0216] 3. Further observation of Application Example 5 shows that when sodium hyaluronate (HA-TLM 3-5S) is missing in the moisturizer, the DPPH free radical scavenging rate and elastase inhibition rate of Application Example 5 both show a significant downward trend compared with Application Example 1. It can be seen that the addition of sodium hyaluronate in the moisturizer is crucial;

[0217] At the same time, it can be seen from Application Example 6 that after further adding sodium hyaluronate (HA-THM) to the skin conditioner, the free radical scavenging ability and elastase inhibition ability of the product are improved. The reason may be that low molecular weight sodium hyaluronate penetrates deep into the skin to replenish the moisture inside the skin, while high molecular weight sodium hyaluronate, as a moisturizer, forms a film on the skin surface to slow down the evaporation of skin moisture. The two work together with other skin conditioners, moisturizers, and antioxidants to further enhance the activity of the skin;

[0218] Further observation of Application Examples 1, 6, and 7 shows that when acetyl hexapeptide-8 and sodium hyaluronate (HA-THM) are used simultaneously in Application Example 6, the effect achieved is significantly better than that in Application Examples 1 and 7. This shows that when acetyl hexapeptide-8 and sodium hyaluronate (HA-THM) are used together, the two produce a good synergistic effect, further enhancing the product's free radical scavenging ability and elastase inhibition ability.

Claims

1. An anti-aging composition containing sheep placenta, characterized in that: By mass, it includes the following components:

2. The anti-aging composition containing sheep placenta according to claim 1, characterized in that: The extraction method of the hydrolyzed sheep placenta extract is as follows: fresh sheep placenta samples are washed and crushed, then mixed with an aqueous solution of inorganic salts, heated, kept warm, and enzymolyzed, and after enzymolysis, the supernatant is taken to obtain the hydrolyzed sheep placenta extract; The inorganic salt is a strong acid and weak base salt; In the enzymolysis process, the enzyme used is at least one of papain, acid protease, neutral protease and bromelain.

3. The anti-aging composition containing sheep placenta according to claim 2, characterized in that: The inorganic salt is selected from at least one of copper sulfate, ammonium sulfate, calcium chloride and magnesium chloride.

4. The anti-aging composition containing sheep placenta according to claim 1, characterized in that: The tremella extract is extracted from the fruiting body part of tremella.

5. The anti-aging composition containing sheep placenta according to claim 1, characterized in that: The amino acid is a basic amino acid, and the basic amino acid is selected from at least one of lysine, histidine and arginine.

6. Use of the anti-aging composition containing sheep placenta as claimed in any one of claims 1 to 5 in preparing cosmetics.

7. A cosmetic, characterized in that: An anti-aging composition containing sheep placenta as claimed in any one of claims 1 to 5 containing 1 to 30 wt%.

8. The cosmetic according to claim 7, characterized in that: It also contains skin conditioners, moisturizers, antioxidants, and chelating agents; The skin conditioning agent is selected from at least one of hydroxyphenylpropionamide benzoic acid, ascorbyl palmitate, acetyl hexapeptide-8, hydroxyethylpiperazine ethane sulfonic acid, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; The moisturizing agent is selected from at least one of butylene glycol, glycerin, 1,2-hexanediol, sodium hyaluronate, and hydrolyzed sodium hyaluronate; The antioxidant is hydroxyacetophenone; The chelating agent is disodium ethylenediaminetetraacetate.

9. The cosmetic according to claim 8, characterized in that: The skin conditioning agent comprises at least acetyl hexapeptide-8.

10. The cosmetic according to claim 8, characterized in that: The sodium hyaluronate in the skin conditioning agent is sodium hyaluronate with a molecular weight of less than 10KDa; The sodium hyaluronate in the moisturizing agent is sodium hyaluronate with a molecular weight greater than or equal to 1000 KDa.

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