Composition with effects of tightening, fading wrinkles and relieving and repairing and application of composition

Abstract

The invention discloses a composition with tightening, wrinkle fading, soothing and repairing effects and application of the composition. The composition with the effects of tightening and fading wrinkles and relieving and repairing is prepared from a lactobacillus fermentation product, hexanediol, fully human collagen, glycosylglycerol, a citrus peel extract, a palmitoyl tripeptide-5 solution, a chrysanthemum extract and SPG beta-glucan. According to the invention, seven active material raw materials including the lactobacillus fermentation product, the fully human collagen, the glycosylglycerol, the citrus peel extract, the palmitoyl tripeptide-5 solution, the chrysanthemum extract and the SPG beta-glucan are creatively combined for use, and the seven raw materials supplement each other and have a synergistic effect; the effects of lightening skin wrinkles, improving skin barriers and relieving skin sensitivity are achieved from multiple dimensions, and cosmetics prepared from the composition have the effects of repairing, relieving, tightening and resisting wrinkles.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology and relates to a composition with the effects of tightening and lightening wrinkles and soothing and repairing and an application thereof. Background Art

[0002] About 36.1% of women in my country have sensitive skin. In the past few years, the skin care market for sensitive skin has developed rapidly. In addition to basic functions such as moisturizing, soothing and repairing, the demand for anti-aging and other functions among sensitive skin groups is also growing. Compared with healthy skin, the barrier function of sensitive skin is relatively weak, the skin barrier is damaged, and the defense ability to the external environment is weak. It is in an inflammatory state for a long time. Inflammatory factors will accelerate the release of free radicals, thereby accelerating the loss of moisture in aging skin. In addition, sensitive skin is often accompanied by inflammatory reactions. Inflammation will activate matrix metalloproteinases, degrade collagen, elastic fibers and other substances in the extracellular matrix and dermis, destroy various supporting tissues of the skin, reduce the elasticity of the skin, accelerate skin aging, and further accelerate skin aging.

[0003] When skin sensitivity and systemic aging problems overlap, sensitive skin will fall into a vicious cycle of fragile skin barrier-external damage-skin aging-aggravated barrier damage, and will be more easily damaged and aged than healthy skin. Therefore, people with sensitive skin with damaged skin barrier need more aging, and the demand for "anti-aging of sensitive skin" care is becoming more and more urgent. They are no longer satisfied with just using products that can soothe sensitive skin. What they are looking for is a comprehensive skin care product developed for sensitive skin, including anti-aging, repair and other functions, so as to achieve comprehensive care, solve and improve skin condition, while the anti-aging products on the market have relatively single functions, and rarely have the functions of anti-aging, soothing and repairing.

[0004] Therefore, there is an urgent need to develop a multi-effect cosmetic composition with the effects of firming and anti-wrinkle, repairing barrier, and soothing sensitivity. Summary of the invention

[0005] In view of the deficiencies in the prior art and actual needs, the present invention provides a composition with the effects of firming, lightening wrinkles, soothing and repairing, and its application. Seven active ingredients, including lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, spiltnia extract and SPGβ-glucan, are combined and used together. The seven ingredients complement each other and synergistically enhance their effectiveness, achieving the effects of lightening skin wrinkles, improving skin barrier, and soothing skin sensitivity from multiple dimensions. The cosmetics made from the composition have the effects of repairing, soothing, firming and anti-wrinkle.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition with the effects of firming, lightening, reducing wrinkles, soothing and repairing skin, wherein the composition with the effects of firming, lightening, reducing wrinkles, soothing and repairing skin comprises lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and SPG β-glucan.

[0008] The present invention creatively combines seven active ingredients, including lactobacillus fermentation products, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, spiltnia extract and SPGβ-glucan. The seven ingredients complement each other and synergistically enhance their effectiveness, achieving the effects of reducing skin wrinkles, improving skin barrier, and soothing skin sensitivity from multiple dimensions. The cosmetics made from the composition have the effects of repairing, soothing, firming and anti-wrinkle.

[0009] Lactobacillus fermentation products have the complete structural efficacy of specific lactobacillus strains, and produce specific interactions with the skin. By downregulating the expression of inflammatory factor IL-8, upregulating the synthesis capacity of filaggrin, etc., they activate the skin's instinctive defense system, achieve anti-inflammatory and soothing effects, repair the barrier, and improve dry skin.

[0010] Collagen is an important structural protein in the human body. It is not only the main component of the extracellular matrix, it also acts as a physical scaffold to maintain the morphology and structure of the skin and tissues and organs. When added to cosmetics, it has the effects of firming, anti-wrinkle, repairing and moisturizing. Fully human collagen includes water, glycerin, sodium dihydrogen phosphate, disodium hydrogen phosphate and soluble collagen.

[0011] Glyceryl glucoside includes glyceryl glucoside, water, and 1,2-pentanediol, which can promote the expression of skin water channel protein AQP3 and enhance the skin's barrier function and moisturizing ability.

[0012] Citrus peel extract can enhance the structure of keratinized cells, making the cells evenly flat and tense, and the intercellular gaps smaller. At this time, the skin has more reflected light, making it more translucent and shiny. In addition, it can reduce transepidermal water loss and enhance the skin barrier function.

[0013] Palmitoyl tripeptide-5 is a signal peptide that stimulates the synthesis of skin collagen and elastin through tissue growth factor (TGF-B), thereby promoting skin cell growth. Palmitoyl tripeptide-5 can also increase the production of elastin, hyaluronic acid, glycosaminoglycans and fibronectin, protect collagen from being decomposed, and achieve the effect of relieving wrinkles and tightening the skin. Palmitoyl tripeptide-5 solution includes palmitoyl tripeptide-5, ethylhexylglycerin, 1,2-hexanediol, polysorbate-20 and water.

[0014] Globenia extract is rich in globulus amide, a natural alternative to botulinum toxin. It can inhibit the release of acetylcholine from the presynaptic membrane, thereby blocking nerve impulses between neuromuscular junctions, relaxing over-contracted muscles, effectively improving dynamic facial wrinkles, and fading expression lines. In addition, Globenia extract is an inhibitor of the pain receptor TRPV1, has a highly effective sedative and soothing effect, and can reduce skin inflammation.

[0015] SPGβ-glucan can activate the activity of skin immune cells and other tissue cells, improve the resistance and repair ability of the entire skin tissue, and also has good skin moisturizing function.

[0016] Preferably, the mass ratio of the lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and SPG β-glucan is (0.1-2):(1-10):(1-10):(1-10):(1-10):(5-10):(5-20).

[0017] The specific point values ​​in the above (0.1-2) can be 0.1, 0.3, 0.5, 0.7, 1, 1.5, 2, etc.

[0018] The specific point values ​​in the above (1-10) can be 1, 2, 3, 5, 7, 8, 9, 10, etc.

[0019] The specific point values ​​in the above (5-10) can be 5, 5.5, 6, 7, 8, 9, 9.5, 10, etc.

[0020] The specific point values ​​in the above (5-20) can be 5, 6, 8, 10, 12, 16, 18, 20, etc.

[0021] In a second aspect, the present invention provides use of the composition with wrinkle tightening, wrinkle lightening, soothing and repairing effects as described in the first aspect in the preparation of cosmetics.

[0022] Preferably, the cosmetics include essence water, toner, emulsion, cream, facial cleanser or facial mask.

[0023] Preferably, the composition having the effects of tightening, lightening, soothing and repairing skin has a mass percentage of 5-30% in the cosmetic, for example, 5%, 6%, 7%, 10%, 20%, 26%, 28%, 30% and the like.

[0024] In a third aspect, the present invention provides a facial cream with firming, wrinkle-lightening, soothing and repairing effects, wherein the facial cream with firming, wrinkle-lightening, soothing and repairing effects comprises: the composition with firming, wrinkle-lightening, soothing and repairing effects described in the first aspect, a moisturizer, a thickener, an emulsifier, oil, a preservative and residual water.

[0025] Preferably, the facial cream with firming, wrinkle-lightening, soothing and repairing effects comprises, by weight: 5 to 30 parts of the composition with firming, wrinkle-lightening, soothing and repairing effects described in the first aspect, 2 to 10 parts of moisturizer, 0.2 to 1.5 parts of thickener, 1 to 4 parts of emulsifier, 5 to 15 parts of oil, 0.1 to 1 part of preservative and the remainder of water.

[0026] The point values ​​among the above 5 to 30 parts can specifically be 5 parts, 6 parts, 8 parts, 10 parts, 20 parts, 25 parts, 28 parts, 30 parts, etc.

[0027] The point values ​​in the above 2 to 10 parts can specifically be selected from 2 parts, 4 parts, 6 parts, 8 parts, 10 parts, etc.

[0028] The point values ​​in the above 0.2 to 1.5 parts may specifically be 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1 part, 1.5 parts, etc.

[0029] The point values ​​in the above 1 to 4 parts can specifically be selected as 1 part, 2 parts, 3 parts, 4 parts, etc.

[0030] The point values ​​among the above 5 to 15 parts can specifically be selected as 5 parts, 6 parts, 8 parts, 10 parts, 8 parts, 10 parts 12 parts, 15 parts, etc.

[0031] The point value in the above 0.1 to 1 part may specifically be 0.2 part, 0.4 part, 0.6 part, 0.8 part, 1 part, etc.

[0032] Preferably, the moisturizing agent includes any one or a combination of at least two of glycerin, glyceryl polyether-26, butylene glycol, 1,3-propylene glycol, sodium hyaluronate, allantoin, rhamnose, trehalose or betaine.

[0033] Preferably, the moisturizing agent is a combination of glycerin, 1,3-propylene glycol and betaine.

[0034] Preferably, the thickener includes any one of ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, sodium polyacryloyldimethyl taurate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6, xanthan gum, sclerotium gum or taro gum, or a combination of at least two thereof.

[0035] Preferably, the emulsifier includes any one or a combination of at least two of sodium stearoyl lactylate, glyceryl stearate, cetearyl glucoside, C12-20 alkyl glucoside, coconut glucoside, C12-20 alkyl glucoside, sucrose stearate or polyglyceryl-10 myristate.

[0036] Preferably, the oil comprises any one of cetearyl alcohol, C14-22 alcohol, behenyl alcohol, squalane, polydimethylsiloxane, dicaprylyl carbonate, caprylic / capric triglyceride or isononyl isononanoate, or a combination of at least two thereof.

[0037] Preferably, the preservative is any one of caprylhydroxamic acid, phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, 1,2-hexanediol, 1,2-pentanediol, 1,2-hexanediol or caprylyl glycol, or a combination of at least two thereof.

[0038] In a fourth aspect, the present invention provides a method for preparing the facial cream having the effects of tightening, lightening wrinkles, soothing and repairing skin according to the third aspect, the preparation method comprising:

[0039] The moisturizer, thickener, skin conditioner and water are mixed and homogenized, and then the mixed matrix is ​​mixed and homogenized with the composition with the wrinkle tightening, wrinkle lightening and soothing repairing effects as described in the first aspect to obtain the product.

[0040] As a preferred technical solution, the preparation method of the facial cream having the effects of tightening and lightening wrinkles and soothing and repairing skin of the present invention comprises the following steps:

[0041] (1) Water phase: Add water, emulsifier and moisturizer into an emulsifying tank, stir and heat to 75-80°C, and then homogenize;

[0042] (2) Oil phase: Add emulsifier and oil into the oil phase pot, heat to 75-80°C, and stir until dissolved until transparent;

[0043] (3) Emulsification: The transparent oil phase obtained in step (2) is pumped into an emulsification tank, vacuum is turned on, homogenization is turned on for emulsification, and after the emulsification is completed, the mixture is kept warm and stirred at 75 to 80° C. for 10 to 20 minutes, and then the temperature is lowered;

[0044] (4) Cooling the mixture to 40-45° C., adding a preservative and the composition having the effects of tightening, lightening, soothing and repairing skin according to claim 1 or 2, and stirring the mixture evenly to obtain the facial cream.

[0045] The specific point values ​​in the above-mentioned 75-80°C may be 75°C, 76°C, 77°C, 78°C, 79°C, 80°C, etc.

[0046] The specific point values ​​in the above 40-45°C may be 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, etc.

[0047] The specific point values ​​in the above 10 to 20 minutes can be 10 minutes, 12 minutes, 16 minutes, 20 minutes, etc.

[0048] Preferably, the homogenization speed in step (1) is 2000-3000 r / min (e.g., 2000 r / min, 2500 r / min, 3000 r / min), and the homogenization time is 5-10 min (e.g., 5 min, 8 min, 10 min).

[0049] Preferably, the pressure of the emulsification tank in step (3) is -0.03 to -0.05 MPa (e.g., -0.03 MPa, -0.04 MPa, -0.05 MPa).

[0050] Preferably, the homogenization speed in step (3) is 2000-3000 r / min (e.g., 2000 r / min, 2500 r / min, 3000 r / min), and the homogenization time is 5-10 min (e.g., 5 min, 8 min, 10 min).

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] The present invention creatively combines and uses seven active raw materials, including lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5, spilion extract, and SPGβ-glucan. The seven raw materials complement each other and synergistically enhance their effectiveness, achieving the effects of reducing skin wrinkles, improving skin barrier, and soothing skin sensitivity from multiple dimensions. The cosmetics made from the composition have the effects of repairing, soothing, firming and anti-wrinkle. DETAILED DESCRIPTION

[0053] To further illustrate the technical means and effects of the present invention, the present invention is further described below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention.

[0054] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0055] The lactobacillus fermentation product involved in the present invention is purchased from Symrise Company, model L19 product; all-human collagen was purchased from Qidian Biotechnology Co., Ltd., model, all-human TM collagen product; glyceryl glucoside was purchased from Xuanjia Company, model NaturalSuperM@2-αGG-40 product; citrus peel extract was purchased from Jiufeng Company, model, Mandarin Clear product; palmitoyl tripeptide-5 solution was purchased from DSM, model, -COLL; Globenia extract was purchased from Gattuso, model GATULINE EXPRESSIONAF; SPGβ-glucan was purchased from Best Pharmaceuticals, model SPG β-glucan products.

[0056] Example 1

[0057] The present embodiment provides a composition with the effects of tightening, reducing wrinkles, soothing and repairing, which includes the following ingredients in parts by weight: 1.5 parts of lactobacillus fermentation product, 7 parts of whole human collagen, 7 parts of glyceryl glucoside, 7 parts of citrus peel extract, 7 parts of palmitoyl tripeptide-5 solution, 15 parts of schizonepeta extract, 15 parts of SPGβ-glucan, 2 parts of hexylene glycol and 38.5 parts of water.

[0058] Example 2

[0059] The present embodiment provides a composition with the effects of tightening, reducing wrinkles, soothing and repairing, which includes the following ingredients in parts by weight: 2 parts of lactobacillus fermentation product, 10 parts of whole human collagen, 10 parts of glyceryl glucoside, 10 parts of citrus peel extract, 10 parts of palmitoyl tripeptide-5 solution, 20 parts of schizonepeta extract, 20 parts of SPGβ-glucan, 2 parts of hexylene glycol and 16 parts of water.

[0060] Example 3

[0061] The present embodiment provides a composition with the effects of tightening, reducing wrinkles and soothing and repairing, which includes the following ingredients in parts by weight: 0.1 parts of lactobacillus fermentation product, 1 part of whole human collagen, 1 part of glyceryl glucoside, 1 part of citrus peel extract, 1 part of palmitoyl tripeptide-5 solution, 5 parts of schizonepeta extract, 5 parts of SPGβ-glucan, 2 parts of hexylene glycol and 83.9 parts of water.

[0062] Example 4

[0063] The present embodiment provides a composition with the effects of tightening, reducing wrinkles and soothing and repairing, which includes the following ingredients in parts by weight: 0.5 parts of lactobacillus fermentation product, 1 part of whole human collagen, 1 part of glyceryl glucoside, 1 part of citrus peel extract, 2 parts of palmitoyl tripeptide-5 solution, 2 parts of schisandra extract, 2 parts of SPGβ-glucan, 2 parts of hexylene glycol and 88.5 parts of water.

[0064] Comparative Example 1

[0065] This comparative example provides a composition with the effects of tightening, lightening lines and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain lactobacillus fermentation product, and its weight proportion is proportionally distributed to the weight of whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and SPG β-glucan.

[0066] Comparative Example 2

[0067] This comparative example provides a composition with the effects of tightening, lightening wrinkles and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain fully human collagen, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and SPG β-glucan.

[0068] Comparative Example 3

[0069] This comparative example provides a composition with the effects of tightening, lightening lines and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain glyceryl glucoside, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, whole human collagen, citrus peel extract, palmitoyl tripeptide-5 solution, schisandra extract and SPG β-glucan.

[0070] Comparative Example 4

[0071] This comparative example provides a composition with the effects of tightening, lightening lines and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain citrus peel extract, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, glyceryl glucoside, whole human collagen, palmitoyl tripeptide-5 solution, schizonepeta extract and SPG β-glucan.

[0072] Comparative Example 5

[0073] This comparative example provides a composition with the effects of tightening, lightening lines and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain palmitoyl tripeptide-5 solution, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, glyceryl glucoside, citrus peel extract, whole human collagen, schisandra extract and SPG β-glucan.

[0074] Comparative Example 6

[0075] This comparative example provides a composition with the effects of tightening, lightening lines and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain schisandra chinensis extract, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, whole human collagen and SPG β-glucan.

[0076] Comparative Example 7

[0077] This comparative example provides a composition with the effects of tightening, lightening wrinkles and soothing and repairing skin. The only difference between it and Example 1 is that it does not contain SPG β-glucan, and its weight proportion is proportionally distributed to the weight of lactobacillus fermentation product, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and whole human collagen.

[0078] Comparative Example 8

[0079] This comparative example provides a composition with the effects of tightening and lightening wrinkles and soothing and repairing skin. The only difference between the comparative example and Example 1 is that the citrus peel extract is replaced by the pomegranate peel extract.

[0080] Comparative Example 9

[0081] This comparative example provides a composition with the effects of tightening and lightening wrinkles and soothing and repairing skin. The difference between the comparative example and Example 1 is that the spiltnia extract is replaced by matricaria extract.

[0082] Comparative Example 10

[0083] This comparative example provides a composition with the effects of tightening and lightening wrinkles and soothing and repairing skin. The only difference between the comparative example and Example 1 is that the SPG β-glucan is replaced by oat extract.

[0084] Test Example 1

[0085] Composition Anti-Wrinkle Testing.

[0086] (1) Elastase is mainly synthesized and secreted by fibroblasts. It is a highly selective and specific proteolytic enzyme. It catalyzes the hydrolysis of carboxyl-containing polypeptide bonds of many amino acids such as glycine, leucine, alanine, and valine. It can decompose elastin in connective tissue protein and cause skin aging. Referring to QTC-C-LAB-TOP-056 "In vitro test method for anti-wrinkle and firming efficacy of cosmetics (elastase inhibition method)", the test concentration is 75 mg / mL, the positive control group is quercetin solution (200 μg / mL), and the negative control group is water. The inhibition rate of elastase by the multi-effect composition with firming, lightening, soothing and repairing effects prepared in each embodiment and comparative example was tested.

[0087] (2) Collagenase plays an important role in the dynamic balance of skin collagen. Its overexpression and abnormal activation are one of the main causes of skin aging. Inhibiting the activity of collagenase can block the degradation of skin collagen and achieve anti-aging effects. The inhibition rate of collagenase in the compositions with firming, wrinkle-lightening and soothing repair effects prepared in the examples and comparative examples was tested. The results are shown in Table 1.

[0088] Table 1

[0089]

[0090]

[0091] Results: The results of Examples 1-3 show that the composition of the present invention with the effects of tightening and lightening wrinkles and soothing and repairing can effectively inhibit collagenase and achieve firming and anti-wrinkle. The results of Example 4 show that the components of the composition of the present invention need to be in a certain ratio to work effectively. Comparative Examples 1-7 show that the 7 raw materials of the composition of the present invention complement each other and synergize to effectively achieve firming and anti-wrinkle. Comparative Examples 8-10 show that the composition of the present invention needs to be in a specific ratio to synergize and effectively achieve firming and anti-wrinkle.

[0092] Test Example 2

[0093] Composition soothing test.

[0094] Hyaluronic acid is a component of the tissue matrix that limits the diffusion of water and other extracellular substances. After being hydrolyzed by hyaluronidase, the cells become non-sticky, causing cell degranulation and exudation of new synthesized media, exerting biological effects and leading to the occurrence of immediate allergic reactions. Hyaluronidase is a participant in allergic reactions and has a strong correlation with allergies. Therefore, the commonly used test substances have always reflected their effects on relieving and improving type I hypersensitivity reactions.

[0095] Experimental steps: 0.1mL CaCl2 solution (0.25mmol / L) and 0.5mL hyaluronidase solution (100μ / mL) were incubated at 37℃ for 20min; 0.5mL sample solution was added and incubated for 5min, 15min, and 20min respectively; 0.5mL sodium hyaluronate solution (0.5g / L) was added, reacted for 30min, then cooled for 5min; 0.1mL sodium hydroxide solution (0.4mol / L) and 0.5mL acetylacetone solution were added. Solution (3.5mL acetylacetone dissolved in 50mL 1.0mol / L sodium carbonate solution), boil in water bath for 15min, then immediately transfer to ice water bath for 5min; add 1mL Ehrlich reagent (0.8g p-dimethylaminobenzaldehyde dissolved in 15mL concentrated hydrochloric acid and 15mL anhydrous ethanol) dropwise, and dilute with 3mL anhydrous ethanol, leave at room temperature for 20min to develop color, measure its absorbance at 540nm, and use 5% dipotassium glycyrrhizinate solution as positive control.

[0096] The hyaluronidase inhibition rate was calculated according to the following formula: Hyaluronidase inhibition rate = {[(AB)-(CD)] / (AB)}×100% Wherein: A is the absorbance of the control solution (using acetate buffer solution instead of the sample solution); B is the absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C is the absorbance of the sample solution; D is the absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0097] The hyaluronidase inhibition rates were calculated after 5 minutes, 15 minutes, and 20 minutes of reaction, respectively. The greater the hyaluronidase inhibition rate, the better the soothing effect of the multi-effect composition for tightening, lightening, soothing and repairing wrinkles. The results are shown in Table 2.

[0098] Table 2

[0099]

[0100]

[0101] Results: The results of Examples 1-3 show that the composition of the present invention with the effects of tightening and lightening wrinkles and soothing and repairing can effectively inhibit hyaluronidase and achieve soothing and repairing. The results of Example 4 show that the components of the composition of the present invention need to be in a certain ratio to effectively play a role. Comparative Examples 1-7 show that the 7 raw materials of the composition of the present invention complement each other, synergize and effectively achieve soothing and repairing. Comparative Examples 8-10 show that the composition of the present invention needs to be in a specific ratio of components to synergize and effectively achieve soothing and repairing.

[0102] Test Example 3

[0103] Human efficacy testing of facial creams.

[0104] Experimental sample: firming, wrinkle-lightening and soothing repairing facial cream, the formula of the facial cream is shown in Table 3.

[0105] Table 3

[0106]

[0107]

[0108] Experimental method: Set up a self-controlled before-and-after test, and use non-invasive testing methods to collect the moisture content of the skin stratum corneum, skin firmness F4 value, skin elasticity R2 value, skin gloss, skin a* value, and skin transepidermal water loss. After taking pictures with Evaskin-S5, analyze the number / volume of nasolabial folds, crow's feet, and under-eye wrinkles, and the texture roughness of the under-eye wrinkles. Also, take VISIA-CR images to evaluate the firming, anti-wrinkle, repairing, soothing, nourishing, and moisturizing effects of the product immediately and after 14 days of use.

[0109] Volunteers: 35 healthy Chinese female subjects with dry, rough, dull, sagging facial skin, sensitive and red facial skin, obvious nasolabial folds, crow's feet, and wrinkles under the eyes were selected. The subjects were aged 30 to 60 years old, with an average age of 45 years old.

[0110] Directions for use: (1) Immediate evaluation: Mask usage: After cleansing, apply 3g of the paste thickly on the face, wait for 20 minutes and then rinse with clean water; (2) 14-day evaluation: Adjust to cream usage: After cleansing, apply 3g of the paste evenly on the entire face, focusing on nasolabial folds, crow's feet, and wrinkles under the eyes. Wait for 20 minutes and then gently massage until absorbed. No need to rinse.

[0111] Use period: 14 days.

[0112] The test contents are shown in Table 4.

[0113] Table 4

[0114]

[0115]

[0116] Parameter description: (1) Wrinkle area / volume: The lower the value, the smaller the wrinkle area / volume. (2) Texture roughness: The lower the value, the smoother the skin. (3) Skin elasticity R2 value: The higher the value, the better the skin elasticity. (4) Skin firmness F4 value: The lower the value, the better the skin firmness. (5) Skin stratum corneum moisture content: The higher the value, the more moisture content of the stratum corneum. (6) Skin gloss: The higher the value, the glossier the skin. (7) Transepidermal water loss: The lower the value, the less transepidermal water loss. The test results are shown in Table 5.

[0117] Table 5

[0118]

[0119]

[0120] Conclusion: The R2 value and F4 value of the test area have been significantly improved compared with before use, indicating that the test sample has a firming effect; the wrinkle area and volume have been significantly improved compared with before use, indicating that the test sample has an anti-wrinkle effect; the transepidermal water loss of the skin in the test area has been significantly improved compared with before use, indicating that the test sample has a repairing effect; the a* value of the skin in the test area has been significantly improved compared with before use, indicating that the test sample has a soothing effect; the skin gloss and skin texture roughness in the test area have been significantly improved compared with before use, indicating that the test sample has a nourishing effect; the moisture content of the stratum corneum in the test area has been significantly improved compared with before use, indicating that the test sample has a moisturizing effect.

[0121] The test cream has multiple effects such as instant and long-lasting firming, anti-wrinkle, moisturizing, repairing, soothing and so on.

[0122] Test Example 4

[0123] Cream safety patch testing.

[0124] The facial cream used in the above-mentioned Test Example 3 was subjected to a human skin patch test in accordance with the Technical Specification for Safety of Cosmetics (2015 edition).

[0125] Subjects: A total of 30 people, including 10 males and 20 females, aged 19 to 55 years old, with an average age of 35 years old, met the voluntary selection criteria for subjects.

[0126] Patch test method: Select qualified patch test equipment and use the closed patch test method. Place about 0.020g~0.025g of the test substance in the patch tester. Apply several strips of low-allergenic tape to the back of the subject to reinforce the patch tester. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0127] The experimental results are shown in Table 6.

[0128] Table 6

[0129]

[0130] The blank control group refers to a facial cream compared with the facial cream used in the above-mentioned test example 3, in which the facial cream does not contain the composition with the effects of tightening, lightening wrinkles and soothing and repairing skin as described in the present invention.

[0131] The test results show that the facial cream prepared by the present invention has no adverse reaction on the skin.

[0132] In summary, the present invention creatively combines seven active ingredients, including lactobacillus fermentation products, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, spiltnia extract and SPGβ-glucan. The seven ingredients complement each other and synergize to achieve the effects of reducing skin wrinkles, improving skin barrier, and soothing skin sensitivity from multiple dimensions. The cosmetics made from the composition have the effects of repairing, soothing, firming and anti-wrinkle.

[0133] The applicant declares that the present invention illustrates the detailed method of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned detailed method, that is, it does not mean that the present invention must rely on the above-mentioned detailed method to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A composition with the effects of tightening and lightening wrinkles and soothing and repairing, characterized in that: The composition with the effects of tightening, lightening, soothing and repairing comprises lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, spiltnia extract and SPG β-glucan.

2. The composition having the effects of tightening, lightening, soothing and repairing wrinkles according to claim 1, characterized in that: The mass ratio of the lactobacillus fermentation product, whole human collagen, glyceryl glucoside, citrus peel extract, palmitoyl tripeptide-5 solution, schizonepeta extract and SPGβ-glucan is (0.1-2):(1-10):(1-10):(1-10):(1-10):(5-10):(5-20).

3. Use of the composition having the effects of tightening, lightening, soothing and repairing skin as claimed in claim 1 or 2 in the preparation of cosmetics.

4. The use according to claim 3, characterized in that: The cosmetics include essence water, lotion, emulsion, cream, facial cleanser or facial mask; Preferably, the composition having the effects of tightening, lightening, soothing and repairing skin has a mass percentage of 5 to 30% in the cosmetic.

5. A facial cream with the effects of tightening and lightening wrinkles and soothing and repairing skin, characterized in that: The facial cream with the effects of tightening, lightening, soothing and repairing comprises: the composition with the effects of tightening, lightening, soothing and repairing according to claim 1 or 2, a moisturizer, a thickener, an emulsifier, oil, a preservative and a balance of water.

6. The facial cream with wrinkle tightening, wrinkle lightening, soothing and repairing effects according to claim 5, characterized in that: The facial cream with the effects of tightening, lightening, soothing and repairing skin comprises, by weight: 5 to 30 parts of the composition with the effects of tightening, lightening, soothing and repairing skin as claimed in claim 1 or 2, 2 to 10 parts of a moisturizer, 0.2 to 1.5 parts of a thickener, 1 to 4 parts of an emulsifier, 5 to 15 parts of oil, 0.1 to 1 part of a preservative and the remainder of water.

7. The facial cream with the effects of tightening, lightening, soothing and repairing according to claim 5 or 6, characterized in that: The moisturizing agent includes any one or a combination of at least two of glycerin, glyceryl polyether-26, butylene glycol, 1,3-propylene glycol, sodium hyaluronate, allantoin, rhamnose, trehalose or betaine; Preferably, the moisturizing agent is a combination of glycerin, 1,3-propylene glycol and betaine.

8. The facial cream with the effects of tightening and lightening wrinkles and soothing and repairing skin according to any one of claims 5 to 7, characterized in that: The thickener includes any one of ammonium acryloyldimethyl taurate / beheneth-25 methacrylate crosspolymer, sodium polyacryloyldimethyl taurate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6, xanthan gum, sclerotium gum or taro gum, or a combination of at least two thereof; Preferably, the emulsifier includes any one or a combination of at least two of sodium stearoyl lactylate, glyceryl stearate, cetearyl glucoside, C12-20 alkyl glucoside, coconut glucoside, C12-20 alkyl glucoside, sucrose stearate or polyglyceryl-10 myristate.

9. The facial cream with the effects of tightening and lightening wrinkles and soothing and repairing skin according to any one of claims 5 to 8, characterized in that: The oil comprises any one or a combination of at least two of cetearyl alcohol, C14-22 alcohol, behenyl alcohol, squalane, polydimethylsiloxane, dicaprylyl carbonate, caprylic / capric triglyceride or isononyl isononanoate; Preferably, the preservative is any one of caprylhydroxamic acid, phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, 1,2-hexanediol, 1,2-pentanediol, 1,2-hexanediol or caprylyl glycol, or a combination of at least two thereof.

10. The method for preparing the facial cream with the functions of tightening, lightening, soothing and repairing skin according to any one of claims 5 to 9, characterized in that: The preparation method comprises the following steps: (1) Water phase: Add water, emulsifier and moisturizer into an emulsifying tank, stir and heat to 75-80°C, and then homogenize; (2) Oil phase: Add emulsifier and oil into the oil phase pot, heat to 75-80°C, and stir until dissolved until transparent; (3) Emulsification: The transparent oil phase obtained in step (2) is pumped into an emulsification tank, vacuum is turned on, homogenization is turned on for emulsification, and after the emulsification is completed, the mixture is kept warm and stirred at 75 to 80° C. for 10 to 20 minutes, and then the temperature is lowered; (5) cooling the mixture to 40-45° C., adding a preservative and the composition having the effects of tightening, lightening, soothing and repairing skin according to claim 1 or 2, and stirring the mixture evenly to obtain the facial cream; Preferably, the homogenization speed in step (1) is 2000-3000 r / min, and the homogenization time is 5-10 min; Preferably, the pressure of the emulsification tank in step (3) is -0.03 to -0.05 MPa; Preferably, the homogenization speed in step (3) is 2000-3000 r / min, and the homogenization time is 5-10 min.

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