Acidified nano sweet orange essential oil growth promoting emulsion as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of plant essential oil emulsification, and particularly relates to an acidified nano sweet orange essential oil growth promoting emulsion as well as a preparation method and application thereof. The growth promoting emulsion is composed of a composite acid preparation and a dispersion phase according to a mass ratio of (24-44): 3. The composite acid preparation is composed of sodium diacetate, calcium propionate and water according to the mass ratio of (1-2): (1-5): (4-16). The dispersion phase is composed of a compound emulsifier and sweet orange essential oil according to a mass ratio of (1-2): 1. The preparation method comprises the following steps: uniformly mixing the composite acid preparation and the dispersion phase according to a mass ratio, shearing to obtain a coarse emulsion, and homogenizing at high pressure to obtain the composite acid-based emulsion. According to the acidified nano sweet orange essential oil growth-promoting emulsion, the organic acid and the sweet orange essential oil are combined for use, the compound emulsifier is rapidly emulsified, and the acidified nano sweet orange essential oil growth-promoting emulsion has good stability and can be used for increasing the body weight of broilers, improving the nutrient digestibility, reducing the content of inflammatory factors in serum and promoting intestinal health when being used in broiler breeding.

Description

Technical Field

[0001] The invention belongs to the technical field of plant essential oil emulsification, and particularly relates to an acidified nano sweet orange essential oil growth-promoting emulsion and a preparation method and application thereof. Background Art

[0002] As people pay more and more attention to food safety, the use of antibiotics is gradually restricted. Natural plant essential oils are widely studied because of their unique biological activity and less side effects. The main components of sweet orange essential oil include active substances such as monoterpenes, aldehydes, monoterpenoids and esters. These components have multiple physiological functions such as antibacterial, anti-inflammatory and antioxidant, which can improve the production performance and disease resistance of animals. Sodium diacetate and calcium propionate can stimulate the secretion of animal digestive enzymes, regulate intestinal health, and improve the digestion and absorption of nutrients by animals. However, sweet orange essential oil is also subject to some limitations in practical applications, such as poor water solubility and high volatility.

[0003] At present, orange essential oil is widely used in shampoo, soap and hand cream. The volatility of orange essential oil leads to its seldom use in livestock and poultry farming. The common use method of orange essential oil in livestock and poultry farming is to mix the essential oil with an adsorbent and feed it to the farmed animals in high doses. This method leads to high cost of orange essential oil and easy volatilization loss. Therefore, it is necessary to provide an orange essential oil with good stability. Summary of the invention

[0004] In view of the problems and shortcomings in the prior art, the present invention aims to provide an acidified nano sweet orange essential oil growth-promoting emulsion and a preparation method thereof.

[0005] In order to achieve the purpose of the present invention, the technical solution adopted by the present invention is as follows:

[0006] The first part of the present invention provides an acidified nano sweet orange essential oil growth-promoting emulsion, which is composed of a composite acid preparation and a dispersed phase in a mass ratio of (24-44):3;

[0007] The composite acid preparation is composed of sodium diacetate, calcium propionate and water in a mass ratio of (1-2):(1-5):(4-16), and the dispersed phase is composed of a composite emulsifier and sweet orange essential oil in a mass ratio of (1-2):1.

[0008] Preferably, the composite emulsifier is composed of an emulsifier, a cosolvent and a stabilizer in a mass ratio of (1-5):(1-3):(1-2).

[0009] Preferably, the growth-promoting emulsion consists of the composite acid preparation and the dispersed phase in a mass ratio of 24:3.

[0010] Preferably, the composite acid preparation is composed of sodium diacetate, calcium propionate and water in a mass ratio of 2:1:5, and the dispersed phase is composed of a composite emulsifier and sweet orange essential oil in a mass ratio of 1:1.

[0011] Preferably, the composite emulsifier is composed of an emulsifier, a cosolvent and a stabilizer in a mass ratio of 5:3:2.

[0012] More preferably, the emulsifier is Tween 80, the cosolvent is 70% edible alcohol, and the stabilizer is sorbitol.

[0013] The second part of the present invention provides a method for preparing the acidified nano sweet orange essential oil growth-promoting emulsion described in the first part, comprising the following steps:

[0014] (1) uniformly mixing an emulsifier, a cosolvent and a stabilizer according to a mass ratio to prepare a composite emulsifier;

[0015] (2) adding sweet orange essential oil to the composite emulsifier according to the mass ratio, and mixing uniformly to obtain a dispersed phase;

[0016] (3) mixing sodium diacetate, calcium propionate and water in a mass ratio to obtain a composite acid preparation;

[0017] (4) mixing the composite acid preparation and the dispersed phase uniformly according to a mass ratio, and shearing to obtain a coarse emulsion;

[0018] (5) The crude emulsion is homogenized under high pressure and cooled in an ice water bath for 0.5 to 3.5 h to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0019] Preferably, the shearing conditions of step (4) are: a shearing speed of 8000 to 12000 r / min, and a shearing time of 3 to 5 min.

[0020] Preferably, the high-pressure homogenization conditions of step (5) are: a pressure of 50 to 100 MPa, and the number of homogenization cycles is 3 to 6 times.

[0021] Preferably, the mixing conditions of steps (1) to (4) are: a rotation speed of 500 to 1000 r / min and a mixing time of 10 to 15 min.

[0022] The third part of the present invention provides an application of the acidified nano sweet orange essential oil growth-promoting emulsion described in the first part in growth-promoting drugs for livestock and poultry breeding.

[0023] Preferably, the method of use of the present invention is mixed drinking, 1 kg of acidified nano sweet orange essential oil growth-promoting emulsion is diluted with 500 kg of water.

[0024] Compared with the prior art, the present invention has the following advantages:

[0025] (1) The acidified nano sweet orange essential oil growth-promoting emulsion of the present invention uses organic acid and sweet orange essential oil in combination, and uses sodium diacetate and calcium propionate as materials for coating the sweet orange essential oil to form acid-coated oil particles. During the preparation of the acidified nano sweet orange essential oil growth-promoting emulsion, a composite emulsifier is used for rapid emulsification to improve the stability of the sweet orange essential oil, and the acidic environment formed by the composite acid preparation further improves the stability of the sweet orange essential oil.

[0026] (2) The acidified nano sweet orange essential oil growth-promoting emulsion of the present invention has a simple preparation process, and the raw and auxiliary materials are easily obtained and highly safe, and is convenient for large-scale production.

[0027] (3) The acidified nano sweet orange essential oil growth-promoting emulsion of the present invention is used in broiler farming, and 1 kg of the acidified nano sweet orange essential oil growth-promoting emulsion is diluted with 500 kg of water. Compared with broilers that do not use the acidified nano sweet orange essential oil growth-promoting emulsion, it can increase the weight of broilers, improve nutrient digestibility, reduce the content of inflammatory factors in serum, and promote intestinal digestion and absorption. When used in livestock and poultry farming, the acidified nano sweet orange essential oil growth-promoting emulsion of the present invention can enhance disease resistance, improve feed utilization efficiency, inhibit the reproduction of harmful bacteria, improve body health, and thus reduce the use of antibiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The present invention is a process flow chart for preparing the acidified nano sweet orange essential oil growth-promoting emulsion. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail by way of embodiments below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0030] Example 1

[0031] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which is as follows: Figure 1 As shown, the following steps are included:

[0032] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0033] (2) According to the mass ratio of composite emulsifier to sweet orange essential oil of 2:1, sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0034] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 1:2:7 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0035] (4) The composite acid preparation and the dispersed phase were mixed at a mass ratio of 40:3 at a rotation speed of 1000 r / min for 10 minutes, and sheared at a speed of 12000 r / min for 3 minutes on a high-speed shearing machine to obtain a coarse emulsion.

[0036] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0037] Example 2

[0038] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0039] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0040] (2) According to the mass ratio of composite emulsifier to sweet orange essential oil of 2:1, sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0041] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 1:5:16 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0042] (4) The composite acid preparation and the dispersed phase were mixed at a mass ratio of 44:3 at a rotation speed of 1000 r / min for 10 minutes, and sheared at a speed of 12000 r / min for 3 minutes on a high-speed shearing machine to obtain a coarse emulsion.

[0043] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0044] Example 3

[0045] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0046] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0047] (2) According to the mass ratio of composite emulsifier to sweet orange essential oil of 2:1, sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0048] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 1:1:4 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0049] (4) The composite acid preparation and the dispersed phase were mixed at a mass ratio of 36:3 at a rotation speed of 1000 r / min for 10 minutes, and sheared at a speed of 12000 r / min for 3 minutes on a high-speed shearing machine to obtain a coarse emulsion.

[0050] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0051] Example 4

[0052] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0053] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0054] (2) Adding sweet orange essential oil to the composite emulsifier in a mass ratio of 2:1, stirring for 10 min at 30° C. and a rotation speed of 1000 r / min, and leaving at room temperature for 15 min to mix evenly, thereby obtaining a dispersed phase (oil phase).

[0055] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 2:1:5 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0056] (4) The composite acid preparation and the dispersed phase were mixed in a mass ratio of 32:3 at a speed of 1000 r / min for 10 min to be uniformly mixed, and sheared on a high-speed shearing machine at a speed of 12000 r / min for 3 min to obtain a coarse emulsion.

[0057] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0058] Example 5

[0059] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0060] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0061] (2) According to the mass ratio of the composite emulsifier to the sweet orange essential oil of 1:1, the sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and a rotation speed of 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0062] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 1:2:7 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0063] (4) The composite acid preparation and the dispersed phase were mixed at a mass ratio of 30:3 at a rotation speed of 1000 r / min for 10 minutes, and sheared at a speed of 12000 r / min for 3 minutes on a high-speed shearing machine to obtain a coarse emulsion.

[0064] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0065] Example 6

[0066] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0067] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0068] (2) According to the mass ratio of the composite emulsifier to the sweet orange essential oil of 1:1, the sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and a rotation speed of 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0069] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 1:1:4 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0070] (4) The composite acid preparation and the dispersed phase were mixed at a mass ratio of 27:3 at a rotation speed of 1000 r / min for 10 minutes, and sheared at a speed of 12000 r / min for 3 minutes on a high-speed shearing machine to obtain a coarse emulsion.

[0071] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0072] Example 7

[0073] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation process of which comprises the following steps:

[0074] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0075] (2) According to the mass ratio of the composite emulsifier to the sweet orange essential oil of 1:1, the sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and a rotation speed of 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0076] (3) Sodium diacetate, calcium propionate and deionized water were mixed in a mass ratio of 2:1:5 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0077] (4) The composite acid preparation and the dispersed phase were mixed in a mass ratio of 24:3 at a speed of 1000 r / min for 10 minutes, and sheared on a high-speed shearing machine at a speed of 12000 r / min for 3 minutes to obtain a coarse emulsion.

[0078] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h and filled to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

[0079] Comparative Example 1

[0080] Comparative Example 1: A microemulsion was prepared by adding sweet orange essential oil and an emulsifier.

[0081] A sweet orange essential oil microemulsion, the preparation method of which comprises the following steps:

[0082] (1) 20 parts of sweet orange essential oil and 10 parts of dispersant isopropyl myristate were uniformly mixed, the rotation speed was 1000r / min, and the mixture was stirred for 10min to obtain a composite sweet orange essential oil, which was then left to stand for use.

[0083] (2) 605 parts of food-grade Span, 5 parts of polyoxyethylene hydrogenated castor oil, 8 parts of ethanol and 8 parts of sodium lauryl sulfate were mixed at a speed of 1000 r / min and stirred for 10 min to prepare a mixed surfactant, which was then allowed to stand for use.

[0084] (3) adding 30 parts of the composite sweet orange essential oil obtained in step (1) to 26 parts of the mixed surfactant obtained in step (2), heating to 68-70° C., rotating speed 1000 r / min, stirring for 10 min to obtain 56 parts of a mixed oil phase, and standing for use.

[0085] (4) Under stirring at 500 r / min, 56 parts of the mixed oil phase obtained in step (3) were added to a 60-65° C. water phase, with 44 parts of drinking water, to obtain a sweet orange essential oil microemulsion.

[0086] Comparative Example 2

[0087] Compared with Example 7, Comparative Example 2 changed the coating material composite acid preparation, and the other preparation processes were the same.

[0088] An acidified nano sweet orange essential oil growth-promoting emulsion, the preparation method of which comprises the following steps:

[0089] (1) Tween 80, 70% edible alcohol and sorbitol were mixed in a mass ratio of 5:3:2 at a speed of 1000 r / min for 10 minutes to obtain a composite emulsifier.

[0090] (2) According to the mass ratio of the composite emulsifier to the sweet orange essential oil of 1:1, the sweet orange essential oil was added to the composite emulsifier, stirred at 30° C. and a rotation speed of 1000 r / min for 10 min, and allowed to stand at room temperature for 15 min to mix evenly to obtain a dispersed phase (oil phase).

[0091] (3) β-glucan, oligofructose and deionized water were mixed in a mass ratio of 2:1:5 at a speed of 1000 r / min for 10 min to obtain a composite acid preparation.

[0092] (4) The composite acid preparation and the dispersed phase were mixed in a mass ratio of 24:3 at a speed of 1000 r / min for 10 minutes, and sheared on a high-speed shearing machine at a speed of 12000 r / min for 3 minutes to obtain a coarse emulsion.

[0093] (5) The crude emulsion was homogenized by a high-pressure homogenizer, and the homogenization cycle was repeated 6 times at a pressure of 100 MPa, and then cooled in an ice water bath for 0.5 h, and filled to obtain an acidified nano sweet orange essential oil emulsion.

[0094] Example 8 Comparative test on the stability of sweet orange essential oil composition

[0095] The properties of the sweet orange essential oil microemulsions prepared in Examples 1 to 7, Comparative Example 1 and Comparative Example 2 of the present invention were compared after being accelerated at 60°C for 10 days. The results are shown in Table 1. The acidified nano sweet orange essential oil growth-promoting emulsions prepared according to the preparation method of the present invention in Examples 1 to 7 and Comparative Example 2 are still clear, transparent and uniform liquids after the accelerated test, with no obvious difference from 0h, and have stable and uniform properties. However, after the conventional sweet orange essential oil in Comparative Example 1 is left standing for a period of time, the system is unstable and slowly stratifies, indicating that the acidified nano sweet orange essential oil growth-promoting emulsion prepared by the method of the present invention has good stability.

[0096] Table 1 Observation on the stability effect of different proportions of sweet orange essential oil compositions

[0097] Grouping Properties (0h) Properties (10d) Example 1 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 2 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 3 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 4 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 5 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 6 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Example 7 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h Comparative Example 1 Clear, transparent and uniform liquid Obvious stratification can be seen, the upper layer is grayish white liquid, the lower layer is yellow oily liquid Comparative Example 2 Clear, transparent and uniform liquid Clear, transparent and uniform liquid, no significant difference from 0h

[0098] Example 9 Effect Test of Acidified Nano Sweet Orange Essential Oil Growth-Promoting Emulsion

[0099] 1. Test materials

[0100] (1) Experimental animals: 480 healthy, uniformly-weighted, 21-day-old white-feathered broiler chickens;

[0101] (2) Feed: Feed composition and nutritional ingredients are shown in Table 2;

[0102] (3) Drug: Acidified nano sweet orange essential oil growth-promoting emulsion prepared in Examples 1 to 7 and Comparative Example 2.

[0103] Table 2 Composition and nutritional value of broiler feed (air-dried basis)

[0104]

[0105] Note: (1) The premix provides the following per kg feed: Vitamin A 12000 IU, Vitamin D 33500 IU, Vitamin E 50 mg, Vitamin K 33 mg, Vitamin B1 3 mg, Vitamin B2 6 mg, Vitamin B6 8 mg, Vitamin B 12 0.05mg, pantothenic acid 30mg, niacin 50mg, folic acid 1.5mg iron 85mg, manganese 59mg, zinc 50mg, copper 15mg, selenium 0.2mg.

[0106] (2) Crude protein is the measured value, and the rest are calculated values.

[0107] 2. Experimental Grouping

[0108] A total of 540 white-feathered broilers were randomly divided into 9 groups, with 6 replicates in each group and 10 birds in each replicate. Control group 1 was fed with feed, control group 2 and growth-promoting emulsion groups 1 to 7 were fed with feed and the acidified nano sweet orange essential oil growth-promoting emulsion prepared by comparative example 2 and examples 1 to 7 was added to the drinking water at a ratio of 1:500. All chickens were fed twice a day and fed freely. Chickens in control group 1 drank water freely, and chickens in other groups drank water in a centralized manner. Immunization and disinfection were carried out according to the conventional procedures for broilers. The ambient temperature was 20-28°C, the relative humidity was 40%-70%, and the feeding conditions were managed in accordance with the "Technical Regulations for Commercial Broiler Production" (GB / T19664-2005). The animal grouping and administration are shown in Table 3. Mixed drinking administration, the administration cycle is 22-42d, and the administration is continuous for 21 days.

[0109] Table 3 Experimental groups and drug administration methods in each group

[0110]

[0111] 3. Test results and analysis

[0112] The evaluation indicators of broiler growth performance include broiler body weight, broiler nutrient digestibility, broiler serum inflammatory factor content and broiler intestinal morphology.

[0113] (1) Effect of acidified nano sweet orange essential oil growth-promoting emulsion on broiler body weight

[0114] The broilers in the control group and the growth-promoting emulsion group were weighed at 21 and 42 days of age, respectively, and the initial body weight (IBW) and final body weight (FBW) were recorded after fasting for 12 hours. The weekly feed addition amount, feed loss amount and residual feed amount were recorded, and the average daily feed intake (ADFI), average daily weight gain (ADG) and feed consumption to weight gain ratio (F / G) of each group in each stage were calculated. The results are shown in Table 4.

[0115] Table 4 Effect of acidified nano sweet orange essential oil growth-promoting emulsion on broiler body weight

[0116]

[0117]

[0118] As shown in Table 4, compared with the control group 1, the growth-promoting emulsion groups 2 to 7 increased the average daily feed intake and average daily weight gain of broilers; compared with the control group 1, the growth-promoting emulsion groups 1 to 3 and 5 to 7 reduced the feed consumption to weight ratio, among which the growth-promoting emulsion group 7 increased the average daily feed intake, average daily weight gain and reduced the feed consumption to weight ratio of broilers. The effect of the acidified nano sweet orange essential oil growth-promoting emulsion prepared in Example 7 on the growth performance of broilers is the best. Compared with the control group 2, the growth-promoting emulsion group 7 has a better effect of increasing the average daily feed intake, average daily weight gain and reducing the feed consumption to weight ratio of broilers. The preparation method of the acidified nano sweet orange essential oil emulsion used in the growth-promoting emulsion group 7 and the control group 2 is similar, except that the composition of the composite acidification preparation is different, indicating that the combined use of the composite acidifier and sweet orange essential oil of the present invention has a better effect on the weight gain of broilers.

[0119] (2) Effect of acidified nano-orange essential oil growth-promoting emulsion on nutrient digestibility of broiler chickens

[0120] The metabolic test was carried out in the late stage of the test (35-38 days of age). A 4-day pre-test was set up before the main test. The pre-test started on the 31st day. One broiler with similar weight was selected from each replicate and placed in a metabolic cage for separate feeding. After a 3-day adaptation period, the broiler was fasted and emptied for 24 hours. The main test started on the 35th day. Feeding started at 08:00 and stopped at 20:00 on the 36th day. Feces were collected from 08:00 on the 36th day and continued for 2 days (48 hours). During the main test, the feed intake and the excrement of the chickens were calculated. After the collection was completed, the chickens were placed in a 70℃ oven to a constant weight, and the excrement was weighed and recorded. After weighing, the chickens were crushed with a grinder to make air-dried samples. The method for preparing air-dried samples of feed was the same as that for preparing fecal samples. The crude protein and crude fat content of fecal samples and feed samples were determined according to GB / T 6432-2018 and GB / T 14772-2008 methods, and the nutrient digestibility was calculated.

[0121] Nutrient apparent digestibility = [(feed intake × feed nutrient content - excrement × excrement nutrient content) / feed intake × feed nutrient content] × 100%. The results are shown in Table 5.

[0122] Table 5 Effect of acidified nano sweet orange essential oil growth-promoting emulsion on apparent nutrient digestibility of broiler chickens

[0123]

[0124]

[0125] As shown in Table 5, compared with the control group 1, the apparent digestibility of crude protein and crude fat in the growth-promoting emulsion groups 1 to 7 is improved, indicating that the acidified nano sweet orange essential oil growth-promoting emulsion can improve the digestion, absorption, and utilization efficiency of nutrients in feed for broilers, among which the growth-promoting emulsion group 7 has better digestion, absorption and utilization effects on crude protein and crude fat in broiler feed nutrients, indicating that the acidified nano sweet orange essential oil growth-promoting emulsion prepared in Example 7 has the highest utilization rate of nutrients in broiler feed. Compared with the control group 2, the apparent digestibility of crude protein and crude fat in the growth-promoting emulsion group 7 is improved, indicating that the combined use of the composite acidulant and sweet orange essential oil of the present invention has the best effect on improving the nutrient digestibility in broiler feed.

[0126] (3) Effects of acidified nano-orange essential oil growth-promoting emulsion on the levels of inflammatory factors in broiler serum

[0127] When the broilers were 42 days old, one broiler with a body weight close to the average body weight of the replicate was selected from each replicate, and 10 mL of blood was collected from the jugular vein in a centrifuge tube, and the serum was separated and divided into test tubes and stored at -20°C for testing. The levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6) in serum were determined by enzyme-linked immunosorbent assay (ELISA). The kit was purchased from Nanjing Jiancheng Bioengineering Research Institute, and the measuring instrument was TECAN GENIOS multifunctional microplate reader. The results are shown in Table 6.

[0128] Table 6 Effects of acidified nano sweet orange essential oil growth-promoting emulsion on the levels of inflammatory factors in broiler serum

[0129] Grouping TNF-α(pg / mL) IL-1β (pg / mL) IL-6 (pg / mL) Control group 1 11.23±3.23 88.54±5.78 4.65±0.12 Control group 2 10.63±3.18 86.68±6.77 4.61±0.28 Growth promoting lotion group 1 10.42±2.17 84.73±6.42 4.59±0.26 Growth promoting lotion group 2 10.75±3.28 86.24±7.55 4.56±0.17 Growth promoting lotion group 3 10.28±2.86 85.38±6.83 4.57±0.11 Growth promoting lotion group 4 9.81±3.68 84.74±8.52 4.51±0.24 Growth promoting lotion group 5 9.68±2.56 83.92±6.44 4.46±0.18 Growth promoting lotion group 6 9.77±2.84 83.82±9.67 4.43±0.32 Growth promoting lotion group 7 9.62±2.72 83.23±7.37 4.41±0.15

[0130] Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine that is mainly produced by monocytes, macrophages and thymus-dependent lymphocytes (T cells). It plays an important role in the regulation of immune responses, T cell-mediated tissue damage, and the occurrence and development of chronic inflammation by binding to its receptors.

[0131] Interleukin 6 (IL-6) is a cytokine in the chemokine family and a type of inflammatory factor. Its main function is to enhance the proliferation and function of cells involved in immune responses. Once an infection occurs, inflammatory cells will produce a large amount of interleukin 6, which promotes the aggregation and function of inflammatory cells.

[0132] Interleukin 1-β (IL-1β) is an important immune system signaling molecule, mainly produced by immune cells. It plays a key role in regulating inflammatory reactions and immune responses. IL-1β can activate other immune cells, promote the inflammatory process, and play a role in infection, tissue damage or autoimmune diseases. Excessive IL-1β may lead to the occurrence of inflammatory diseases.

[0133] As shown in Table 6, compared with the control group 1, the growth-promoting emulsion groups 1 to 7 all reduced the content of inflammatory factors in the serum and enhanced the anti-inflammatory effect of broilers. Among them, the growth-promoting emulsion group 7 reduced the content of inflammatory factors in the serum the most, indicating that the acidified nano sweet orange essential oil growth-promoting emulsion prepared in Example 7 has the best effect on promoting anti-inflammatory effects in broilers. Compared with the control group 2, the content of inflammatory factors in the serum of the growth-promoting emulsion group 7 was significantly reduced, indicating that the combined use of the composite acidifier and sweet orange essential oil of the present invention has a better effect on promoting anti-inflammatory effects in broilers.

[0134] (4) Effects of acidified nano-orange essential oil growth-promoting emulsion on intestinal morphology of broiler chickens

[0135] When the broilers were 42 days old, one broiler with a body weight close to the average body weight of the replicate was selected from each replicate. After slaughter, the duodenum and jejunum segments of about 1 cm were taken, the contents of the intestinal segments were slowly rinsed with normal saline, and the intestinal segments were preserved in formalin solution for fixation. The fixed samples were embedded in paraffin, stained with hematoxylin-eosin (HE) and photographed after embedding. The villus length and crypt depth were measured using the Motic Images Advanced 3.2 pathological image analysis system. The results are shown in Table 7.

[0136] Table 7 Effects of acidified nano sweet orange essential oil growth-promoting emulsion on intestinal morphology of broiler chickens

[0137]

[0138] The edge of the villi in the intestine of animals can secrete a variety of digestive enzymes. Therefore, the longer the villi, the stronger the digestion and absorption capacity of the animal. The depth of the crypt determines the speed of the mitosis of the intestinal villi to generate epithelial cells, reflecting the cell generation rate. The shallower the crypt, the higher the cell maturation rate and the enhanced secretion function. Therefore, the ratio of villus height to crypt depth can comprehensively reflect the digestion and absorption function of the small intestine. If the ratio decreases, the mucosa is likely to be damaged, the digestion and absorption capacity decreases, and diarrhea and growth retardation are often accompanied. If the ratio increases, the number of intestinal epithelial cells increases, the intestinal absorption area increases, and the absorption and utilization rate of nutrients is improved, which is also conducive to the synthesis of functional substances.

[0139] As shown in Table 7, compared with the control group 1, the growth-promoting emulsion groups 1 to 7 all have the effect of increasing the intestinal villus height of the duodenum and jejunum, the ratio of villus height to crypt depth, and reducing the crypt depth, indicating that the acidified nano sweet orange essential oil growth-promoting emulsions can promote intestinal digestion and absorption, among which the growth-promoting emulsion group 7 has the best effect, indicating that the acidified nano sweet orange essential oil growth-promoting emulsion prepared in Example 7 has the best effect on promoting intestinal health. Compared with the control group 2, the growth-promoting emulsion group 7 has a more obvious effect of increasing the intestinal villus height of the duodenum and jejunum, the ratio of villus height to crypt depth, and reducing the crypt depth, indicating that the combined use of the composite acidulant and sweet orange essential oil of the present invention promotes the intestinal health of broilers.

[0140] In summary, the acidified nano sweet orange essential oil growth-promoting emulsion prepared by the present invention has good stability. At the same time, the combined use of the organic acid and sweet orange essential oil of the present invention has a better growth-promoting effect than the use of other acid preparations.

[0141] The above embodiments are intended to illustrate the essential content of the present invention, but are not intended to limit the protection scope of the present invention. Those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and protection scope of the technical solution of the present invention.

Claims

1. An acidified nano sweet orange essential oil growth-promoting emulsion, characterized in that: It is composed of a composite acid preparation and a dispersed phase in a mass ratio of (24-44):3; The composite acid preparation is composed of sodium diacetate, calcium propionate and water in a mass ratio of (1-2):(1-5):(4-16), and the dispersed phase is composed of a composite emulsifier and sweet orange essential oil in a mass ratio of (1-2):

1.

2. The acidified nano sweet orange essential oil growth-promoting emulsion according to claim 1, characterized in that: The composite emulsifier is composed of an emulsifier, a cosolvent and a stabilizer in a mass ratio of (1-5):(1-3):(1-2).

3. The acidified nano sweet orange essential oil growth-promoting emulsion according to claim 1, characterized in that The growth-promoting emulsion consists of a composite acid preparation and a dispersed phase in a mass ratio of 24:

3.

4. The acidified nano sweet orange essential oil growth-promoting emulsion according to claim 3, characterized in that: The composite acid preparation is composed of sodium diacetate, calcium propionate and water in a mass ratio of 2:1:5, and the dispersed phase is composed of a composite emulsifier and sweet orange essential oil in a mass ratio of 1:

1.

5. The acidified nano sweet orange essential oil growth-promoting emulsion according to claim 2, wherein the emulsifier is Tween 80, the cosolvent is 70% edible alcohol, and the stabilizer is sorbitol.

6. The method for preparing the acidified nano sweet orange essential oil growth-promoting emulsion according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) uniformly mixing an emulsifier, a cosolvent and a stabilizer according to a mass ratio to prepare a composite emulsifier; (2) adding sweet orange essential oil to the composite emulsifier according to the mass ratio, and mixing uniformly to obtain a dispersed phase; (3) mixing sodium diacetate, calcium propionate and water in a mass ratio to obtain a composite acid preparation; (4) mixing the composite acid preparation and the dispersed phase uniformly according to a mass ratio, and shearing to obtain a coarse emulsion; (5) The crude emulsion is homogenized under high pressure and cooled in an ice water bath for 0.5 to 3.5 h to obtain an acidified nano sweet orange essential oil growth-promoting emulsion.

7. The preparation method according to claim 6, characterized in that: The shearing conditions of step (4) are as follows: the shearing speed is 8000-12000 r / min, and the shearing time is 3-5 min.

8. The preparation method according to claim 6, characterized in that: The high-pressure homogenization conditions of step (5) are: pressure of 50 to 100 MPa, and the number of homogenization cycles is 3 to 6 times.

9. The preparation method according to claim 6, characterized in that: The mixing conditions of steps (1) to (4) are as follows: a rotation speed of 500 to 1000 r / min and a stirring time of 10 to 15 min.

10. Use of the acidified nano sweet orange essential oil growth-promoting emulsion according to any one of claims 1 to 5 in growth-promoting drugs for livestock and poultry breeding.

Images