Preparation method of water-soluble curcumin composite powder

Abstract

The invention discloses a preparation method of water-soluble curcumin composite powder, and belongs to the technical field of curcumin. The preparation method comprises the following steps: S1, preparing the following raw materials in parts by weight: 10-50 parts of curcumin, 1-10 parts of a biological surfactant, 3-9 parts of soluble dietary fibers, 1-8 parts of water-soluble vegetable proteins and 100-200 parts of water; s2, dissolving a biological surfactant, soluble dietary fibers and water-soluble vegetable proteins in water, uniformly mixing and stirring, adding curcumin, and continuously uniformly mixing to obtain a mixed solution; s3, the mixed solution is subjected to spray drying, and water-soluble curcumin composite powder is obtained. The prepared curcumin composite powder has better water solubility, stability, oxidation resistance and bioavailability, and application of curcumin in the fields of food and medicine is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of curcumin, and more specifically to a method for preparing a water-soluble curcumin composite powder. Background Art

[0002] Curcumin has a wide range of biological and pharmacological activities. In the food industry, it is often used as a food coloring for the production and processing of pasta products, meat products and functional beverages. While increasing the color and flavor of food, it can also play a role in promoting the health of the body. In the medical field, it has the potential to fight cancer, thrombosis, heart failure, inhibit inflammation and lower blood pressure, and has high pharmacological application value. However, curcumin has extremely low solubility in water, and its stability after being made into an aqueous solution is also poor. It is difficult to be absorbed by the human body and has low bioavailability, which greatly limits the application of curcumin in food and medicine. Summary of the invention

[0003] The object of the present invention is to overcome the deficiencies of the prior art and provide a method for preparing a water-soluble curcumin composite powder.

[0004] In order to achieve the above object, the present invention adopts the following technical solution:

[0005] A method for preparing a water-soluble curcumin composite powder comprises the following steps:

[0006] S1. Prepare the following raw materials in parts by weight: 10-50 parts of curcumin, 1-10 parts of biosurfactant, 3-9 parts of soluble dietary fiber, 1-8 parts of water-soluble plant protein, and 100-200 parts of water;

[0007] S2, dissolving the biosurfactant, soluble dietary fiber and water-soluble plant protein in water, mixing and stirring evenly, adding curcumin and continuing to mix evenly to obtain a mixed solution;

[0008] S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.

[0009] The present invention is further configured such that the biosurfactant is at least one of steviol glycoside, polyvinyl pyrrolidone, hydroxypropyl methylcellulose, and polyethylene glycol.

[0010] The present invention is further configured such that the biosurfactant is a composition of hydroxypropyl methylcellulose and steviol glycoside.

[0011] The present invention is further configured such that the biosurfactant is a composition of hydroxypropyl methylcellulose and steviol glycoside in a mass ratio of 0.5-1:1.

[0012] The present invention is further configured that the soluble dietary fiber is oat soluble dietary fiber.

[0013] The present invention is further configured such that the water-soluble plant protein is pea protein.

[0014] The present invention is further configured to include the following steps:

[0015] S1. Prepare the following raw materials in parts by weight: 25 parts of curcumin, 4.5 parts of biosurfactant, 3.2 parts of soluble dietary fiber, 3 parts of water-soluble plant protein, and 120 parts of water; wherein the biosurfactant is a composition of 1.5 parts of hydroxypropyl methylcellulose and 3 parts of steviol glycosides, the soluble dietary fiber is oat soluble dietary fiber, and the water-soluble plant protein is pea protein;

[0016] S2, dissolving the biosurfactant, soluble dietary fiber and water-soluble plant protein in water, mixing and stirring evenly, adding curcumin and continuing to mix evenly to obtain a mixed solution;

[0017] S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.

[0018] In summary, the present invention has the following beneficial effects:

[0019] By rationally compounding curcumin with biosurfactants, soluble dietary fiber, and water-soluble plant protein, a water-soluble curcumin composite powder with good water solubility, stability, antioxidant properties and bioavailability is obtained. Especially when steviol glycosides, oat soluble dietary fiber, and pea protein are added for compounding, its stability and antioxidant properties can be further significantly improved. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The preparation method of the water-soluble curcumin composite powder of the present invention comprises the following steps:

[0022] S1. Prepare the following raw materials in parts by weight: 10-50 parts of curcumin, 1-10 parts of biosurfactant, 3-9 parts of soluble dietary fiber, 1-8 parts of water-soluble plant protein, and 100-200 parts of water;

[0023] The biosurfactant is at least one of steviol glycoside, polyvinyl pyrrolidone, hydroxypropyl methylcellulose and polyethylene glycol, preferably a combination of hydroxypropyl methylcellulose and steviol glycoside, more preferably a combination of hydroxypropyl methylcellulose and steviol glycoside at a mass ratio of 0.5-1:1. The soluble dietary fiber is oat soluble dietary fiber. The water-soluble plant protein is pea protein.

[0024] S2. Dissolve the biosurfactant, soluble dietary fiber and water-soluble plant protein in water, mix and stir evenly, then add curcumin and continue to mix evenly to obtain a mixed solution.

[0025] S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.

[0026] The preferred embodiments are as follows:

[0027] Example 1

[0028] S1. Prepare the following raw materials in parts by weight: 25 parts of curcumin, 4.5 parts of biosurfactant (including 1.5 parts of hydroxypropyl methylcellulose and 3 parts of steviol glycosides), 3.2 parts of oat soluble dietary fiber, 3 parts of pea protein, and 120 parts of water;

[0029] Pea protein is hydrophobic. When curcumin is mixed with hydroxypropyl methylcellulose, steviol glycosides, oat soluble dietary fiber and pea protein, curcumin can gradually enter the hydrophobic domain of pea protein due to the hydrophobic effect. The addition of polyvinyl pyrrolidone, steviol glycosides and oat soluble dietary fiber can make the interaction between curcumin and the hydrophobic domain of protein more stable.

[0030] S2. Dissolve the biosurfactant, oat soluble dietary fiber and pea protein in water, mix and stir evenly, then add curcumin and continue to mix evenly to obtain a mixed solution.

[0031] S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.

[0032] Example 2

[0033] The curcumin composite powder was prepared according to the method of Example 1, except that the mass of hydroxypropyl methylcellulose in step S1 of Example 1 was replaced by steviol glycoside.

[0034] Example 3

[0035] The curcumin composite powder was prepared according to the method of Example 1, except that the mass of steviol glycoside in step S1 of Example 1 was replaced by hydroxypropyl methylcellulose.

[0036] Example 4

[0037] The curcumin composite powder was prepared according to the method of Example 1, except that the mass of steviol glycoside in step S1 of Example 1 was replaced by polyvinyl pyrrolidone.

[0038] Comparative Example 1

[0039] The curcumin composite powder was prepared according to the method of Example 1, except that the mass of oat soluble dietary fiber in step S1 of Example 1 was replaced by water.

[0040] Comparative Example 2

[0041] The curcumin composite powder was prepared according to the method of Example 1, except that the mass of pea protein in step S1 of Example 1 was replaced by water.

[0042] The performance tests were performed on the curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2:

[0043] (1) The curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 were dissolved in ultrapure water until the aqueous solution reached a saturated state, and the absorbance of curcumin in the saturated solution was measured at 425 nm using an ultraviolet-visible spectrophotometer with ultrapure water as a reference, and the solubility of curcumin in the saturated solution was calculated. The test results show that compared with the maximum solubility of the curcumin raw material used in the embodiments of the present invention in an equal amount of ultrapure water under the same conditions, the solubility of curcumin in the saturated solution of the curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 was increased by 117 times, 113 times, 85 times, 88 times, 93 times, and 90 times, respectively.

[0044] (2) The curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 were respectively taken and added to water to prepare a curcumin composite powder aqueous solution with a final curcumin concentration of 10 μg / mL, and the aqueous solutions were placed at room temperature and away from light. After testing, it was found that the aqueous solutions of the curcumin composite powders prepared in Examples 1 to 2 were still uniform and no precipitation was precipitated after being placed for 360 days, while the aqueous solutions of the curcumin composite powders prepared in Examples 3 to 4 and Comparative Examples 1 to 2 had a precipitation of 220 days, 250 days, 200 days, and 230 days, respectively.

[0045] (3) Take the curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2, respectively, add them to water, prepare a curcumin composite powder aqueous solution with a final curcumin concentration of 10 μg / mL, measure the absorbance of each solution at 425 nm, and calculate the DPPH radical scavenging rate. The results show that the DPPH radical scavenging rates of the curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 are 85.1%, 86.5%, 70.6%, 71.1%, 73.2%, and 71.8%, respectively.

[0046] (4) The curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 were evaluated for in vivo absorption in rats. The test method was as follows: SD male rats were selected and gavage was performed on rats in each group (n=5) at a dose of 200 mg / kg. Blood was collected from the orbits at 0.33, 0.66, 1.0, 1.5, 2, 3, 4, 6, 8, 12, and 24 h after gavage. The blood samples were centrifuged at 4000 rpm for 10 min, 200 μL of the supernatant was taken, 10 μL of a 30 μg / L rhamnosine ethanol solution was added as an internal standard, 1 mL of acetonitrile was added, vortexed for 3 min, and centrifuged at 10000 rpm for 10 min. The supernatant was taken and heated at 40° C. for 1 h. 2 Blow dry, add 100 μL mobile phase (phase A: phase B = 50:50) to dissolve, centrifuge at 10000rpm for 10min, take 20 μL of supernatant and inject it for gradient elution detection by HPLC, and calculate the bioavailability of curcumin. HPLC detection conditions: chromatographic column: C18 column, filler Hypersil ODS2 (5μm, 4.6mm×250mm), mobile phase: acetonitrile (phase A), 5% acetic acid aqueous solution (phase B); elution mode: 0~6min: 40%→65%A; 6~15min: 65%A; 15~15.5min: 65%→40%A; 15.5~20min: 40%A. Flow rate: 1mL / min; column temperature: 30℃; detection wavelength: 430nm. The results showed that the bioavailability of the curcumin composite powders prepared in Examples 1 to 4 and Comparative Examples 1 to 2 were 56.2%, 55.1%, 51.3%, 52.9%, 49.5% and 50.6%, respectively.

[0047] The above experimental results show that the water-soluble curcumin composite powder prepared by the present invention has good water solubility, stability, antioxidant properties and bioavailability. At the same time, it is found that when steviol glycosides, oat soluble dietary fiber and pea protein are added for compounding, steviol glycosides, oat soluble dietary fiber and pea protein have a synergistic effect, which can further significantly improve the stability and antioxidant properties of the curcumin composite powder.

[0048] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing a water-soluble curcumin composite powder, characterized in that: The following steps are involved: S1. Prepare the following raw materials in parts by weight: 10-50 parts of curcumin, 1-10 parts of biosurfactant, 3-9 parts of soluble dietary fiber, 1-8 parts of water-soluble plant protein, and 100-200 parts of water; S2, dissolving the biosurfactant, soluble dietary fiber and water-soluble plant protein in water, mixing and stirring evenly, adding curcumin and continuing to mix evenly to obtain a mixed solution; S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.

2. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The biosurfactant is at least one of steviol glycoside, polyvinyl pyrrolidone, hydroxypropyl methylcellulose and polyethylene glycol.

3. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The biosurfactant is a composition of hydroxypropyl methylcellulose and steviol glycoside.

4. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The biosurfactant is a composition of hydroxypropyl methylcellulose and steviol glycoside in a mass ratio of 0.5-1:

1.

5. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The soluble dietary fiber is oat soluble dietary fiber.

6. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The water-soluble plant protein is pea protein.

7. A method for preparing a water-soluble curcumin composite powder according to claim 1, characterized in that: The following steps are involved: S1. Prepare the following raw materials in parts by weight: 25 parts of curcumin, 4.5 parts of biosurfactant, 3.2 parts of soluble dietary fiber, 3 parts of water-soluble plant protein, and 120 parts of water; wherein the biosurfactant is a composition of 1.5 parts of hydroxypropyl methylcellulose and 3 parts of steviol glycosides, the soluble dietary fiber is oat soluble dietary fiber, and the water-soluble plant protein is pea protein; S2, dissolving the biosurfactant, soluble dietary fiber and water-soluble plant protein in water, mixing and stirring evenly, adding curcumin and continuing to mix evenly to obtain a mixed solution; S3, spray drying the mixed solution to obtain water-soluble curcumin composite powder.