Dendrobium officinale fermentation liquor composition with blood sugar reducing effect as well as preparation method and application of dendrobium officinale fermentation liquor composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a dendrobium officinale fermentation liquor composition with a blood sugar reducing effect and a preparation method and application thereof. The composition comprises dendrobium officinale fermentation liquor and tremella aurantialba fermentation liquor or dendrobium officinale fermentation liquor and ganoderma lucidum fermentation liquor, the volume ratio of the dendrobium officinale fermentation liquor to the tremella aurantialba fermentation liquor or the ganoderma lucidum fermentation liquor is 1: (0.5-2), and the obtained composition has very high alpha-glucosidase inhibition rate.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a Dendrobium officinale fermentation liquid composition with blood sugar lowering effect, a preparation method and application thereof. Background Art

[0002] Recent studies have shown that the active ingredients in Ganoderma lucidum, such as polysaccharides, triterpenoids and amino acid peptides, have antioxidant, hypoglycemic and immunomodulatory effects. Ganoderma lucidum polysaccharides can terminate free radical chain reactions, improve cell viability, reshape the activity of endogenous antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT), thereby reducing the content of oxidation-related end products such as lactate dehydrogenase (LDH) and malondialdehyde (MDA), and play an antioxidant role. Ganoderma lucidum polysaccharides can regulate the metabolism of carbohydrates and lipids in the body, improve insulin sensitivity, reduce the level of pancreatic β-cell apoptosis, avoid insulin resistance, and control the body's blood sugar level in a stable state. Ganoderma lucidum triterpenoid E and ganoderic acid F protect cells from oxidative damage caused by H2O2 by scavenging 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) and oxygen free radical absorbance capacity (ORAC); ganoderic acid can play a hypoglycemic role by inhibiting the activity of α-glucosidase.

[0003] Tremella fuciformis is an edible and medicinal fungus with high nutritional and medicinal value. It has multiple biological activities such as lowering blood sugar, lowering blood lipids, anti-oxidation, anti-inflammatory, anti-coagulation, and enhancing immunity. Studies have shown that Tremella fuciformis polysaccharides have strong antioxidant activity and can effectively reduce the negative impact of free radicals on cells. Tremella fuciformis fermentation broth polysaccharides have a certain reducing ability and strong activity in scavenging DPPH and hydroxyl free radicals. The polysaccharide TAP and the decomposition product H-TAP in Tremella fuciformis fruiting body can accelerate the metabolism of glucose in the blood; Tremella fuciformis fruiting body polysaccharides, mycelium polysaccharides, and extracellular polysaccharides all have the function of lowering blood sugar.

[0004] The main functional components of Dendrobium candidum include polysaccharides, flavonoids, alkaloids, bibenzyl, amino acids and trace elements. Dendrobium candidum polysaccharides can remove excess hydroxyl radicals and superoxide anion radicals produced by pyrogallol under alkaline conditions, significantly increase the activity of superoxide dismutase and glutathione peroxidase in mouse serum and liver tissue, reduce the content of malondialdehyde, and have significant in vitro antioxidant effects at the organelle and tissue levels. Many studies have shown that Dendrobium candidum can improve sugar and lipid metabolism, and its mechanisms include improving insulin sensitivity, promoting glycogen synthesis, increasing the body's energy metabolism, and promoting the secretion of incretin. It can lower the body's blood sugar level, improve diabetes, and has no effect on the blood sugar level of normal bodies.

[0005] Ganoderma lucidum, Tremella fuciformis and Dendrobium candidum all have antioxidant and hypoglycemic effects, but their mechanisms of action may be different. In response to the diseases and problems related to people's irregular work and rest schedules, poor sleep quality, and increasing age, which lead to reduced physical fitness, accelerated aging, abnormal blood sugar and blood lipid levels, and there are few reports on the hypoglycemic function of these three ingredients in combination. Natural plant and fungal extracts / fermentations are considered to have great potential in the auxiliary treatment of diabetes due to their high safety and few side effects. By scientifically matching a variety of plant extracts with hypoglycemic activity to prepare auxiliary blood sugar-lowering health foods, a new treatment option can be provided for diabetic patients. Summary of the invention

[0006] To this end, the present invention provides a Dendrobium officinale fermentation liquid composition with blood sugar lowering effect. The composition uses Dendrobium officinale fermentation liquid as a raw material and is supplemented with Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid. The obtained composition has a good α-glucosidase inhibition rate.

[0007] The technical contents of the present invention are:

[0008] On the one hand, the present invention provides a Dendrobium officinale fermentation liquid composition, comprising Dendrobium officinale fermentation liquid and Tremella fuciformis fermentation liquid or Dendrobium officinale fermentation liquid and Ganoderma lucidum fermentation liquid, wherein the volume ratio of Dendrobium officinale fermentation liquid to Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid is 1: (0.5-2).

[0009] Preferably, the volume ratio of the Dendrobium officinale fermentation liquid to the Tremella fuciformis fermentation liquid or the Ganoderma lucidum fermentation liquid is 1:(1-2).

[0010] More preferably, the volume ratio of the Dendrobium officinale fermentation liquid to the Tremella fuciformis fermentation liquid is 1:1 or 1:2; the volume ratio of the Dendrobium officinale fermentation liquid to the Ganoderma lucidum fermentation liquid is 1:1 or 1:2.

[0011] The Dendrobium officinale fermented liquid is prepared by mixing Dendrobium officinale and water, performing enzymatic hydrolysis, and inoculating Lactobacillus plantarum ACCC11016 for fermentation, wherein the Lactobacillus plantarum is the Lactobacillus plantarum with the preservation number of ACCC 11016.

[0012] Preferably, the enzyme preparation selected for enzymatic hydrolysis of Dendrobium officinale is cellulase; the added amount of the cellulase is 3-5%; more preferably, the added amount of the cellulase is 4%.

[0013] More preferably, the enzymatic activity of the cellulase is 3000-4000 U / g, and even more preferably, the enzymatic activity of the cellulase is 3500 U / g.

[0014] Preferably, the inoculation amount of Lactobacillus plantarum ACCC 11016 is 1-3%; more preferably, the inoculation amount of Lactobacillus plantarum ACCC 11016 is 2%; more preferably, the bacterial viability of Lactobacillus plantarum ACCC 11016 is 1×10 8 CFU / mL and above.

[0015] Preferably, the solid-liquid ratio of the Dendrobium officinale and water is 1:(10-20); more preferably, the solid-liquid ratio of the Dendrobium officinale and water is 1:15.

[0016] Preferably, the preparation method of the Dendrobium officinale fermentation liquid is as follows: (1) drying and crushing the Dendrobium officinale and mixing it with water, adding cellulase to hydrolyze it and then inactivate it to obtain an enzymatic solution; (2) inoculating Lactobacillus plantarum with a preservation number of ACCC 11016 into the enzymatic solution for fermentation and sterilization, and separating the solid and liquid, and the supernatant is the Dendrobium officinale fermentation liquid.

[0017] Further preferably, the enzymolysis temperature is 40-60°C, and the enzymolysis time is 3-5h; further preferably, the enzymolysis temperature is 50°C, and the enzymolysis time is 4h.

[0018] Further preferably, the inactivation temperature is 80-100°C and the time is 5-15 min; further preferably, the inactivation temperature is 90°C and the time is 10 min.

[0019] More preferably, the fermentation temperature is 35-40°C, and the fermentation time is 48-72h; even more preferably, the fermentation temperature is 37°C, and the fermentation time is 48h.

[0020] The Ganoderma lucidum fermented liquid is prepared by inoculating Lactobacillus acidophilus CGMCC1.12735 with the supernatant obtained by enzymolysis, enzyme inactivation and solid-liquid separation after mixing Ganoderma lucidum and water, wherein the Lactobacillus acidophilus is the Lactobacillus acidophilus with the preservation number of CGMCC1.12735.

[0021] Preferably, the inoculation amount of Lactobacillus acidophilus CGMCC1.12735 is 1-3%; further preferably, the inoculation amount of Lactobacillus acidophilus CGMCC1.12735 is 2%.

[0022] More preferably, the viable count of Lactobacillus acidophilus CGMCC1.12735 is 1×10 8 CFU / mL and above.

[0023] Preferably, the enzyme preparation selected for enzymolysis includes papain, cellulase and pectinase.

[0024] Preferably, the added amount of the enzyme preparation is 0.5-1.0%; further preferably, the added amount of the enzymatic hydrolysis is 0.8%; further preferably, the weight ratio of papain, cellulase and pectinase is 1:2:2.

[0025] Preferably, the enzymatic activity of the cellulase is 3000-4000 U / g, the enzymatic activity of the pectinase is 10,000-30,000 U / g, and the enzymatic activity of the papain is 80,000-120,000 U / g; further preferably, the enzymatic activity of the cellulase is 3500 U / g, the enzymatic activity of the pectinase is 20,000 U / g; and the enzymatic activity of the papain is 100,000 U / g.

[0026] Preferably, the solid-liquid ratio of the Ganoderma lucidum to water is 1:(10-20); more preferably, the solid-liquid ratio of the Ganoderma lucidum fruiting body to water is 1:15.

[0027] Preferably, the preparation method of the Ganoderma lucidum fermentation liquid is as follows: (1) adding an enzyme preparation to a mixture of Ganoderma lucidum and water for enzymolysis and inactivating the enzyme to obtain a Ganoderma lucidum hydrolyzate; (2) separating the Ganoderma lucidum hydrolyzate from solid and liquid, inoculating the obtained supernatant with Lactobacillus acidophilus CGMCC1.12735 for fermentation, separating the solid and liquid, and obtaining the Ganoderma lucidum fermentation liquid through solid-liquid separation.

[0028] Further preferably, the enzymatic hydrolysis conditions are: enzymatic hydrolysis temperature 40-60°C, enzymatic hydrolysis time 2-4h; further preferably, enzymatic hydrolysis temperature 50°C, enzymatic hydrolysis time 3h.

[0029] Further preferably, the enzyme inactivation conditions are: enzyme inactivation temperature 90-100°C, time 8-12 min; further preferably, the enzyme inactivation temperature 90°C, time 10 min.

[0030] More preferably, the fermentation temperature is 35-40°C, and the fermentation time is 48-72h; even more preferably, the fermentation temperature is 37°C, and the fermentation time is 60h.

[0031] The Tremella fuciformis fermented liquid is prepared by extracting Tremella fuciformis and water and inoculating Lactobacillus plantarum for fermentation, wherein Lactobacillus plantarum CGMCC1.1856 is the Lactobacillus plantarum with the preservation number of CGMCC1.1856.

[0032] Preferably, the solid-liquid ratio of the golden ear to water is 1:(40-60), and further preferably, the solid-liquid ratio of the golden ear to water is 1:50.

[0033] Preferably, the inoculation amount of Lactobacillus plantarum CGMCC1.1856 is 1-3%, and further preferably, the inoculation amount of Lactobacillus plantarum CGMCC1.1856 is 2%; further preferably, the viable count of Lactobacillus plantarum CGMCC1.1856 is 1×108 CFU / mL and above.

[0034] Preferably, the preparation method of the golden ear fermentation liquid is as follows: (1) fresh golden ear is cut into pieces, mixed evenly with water in a solid-liquid ratio of 1: (40-60), and then extracted to obtain a golden ear water extract; (2) the golden ear water extract is sterilized and then inoculated with Lactobacillus plantarum CGMCC1.1856 for fermentation. After the fermentation is completed, the solid and liquid are separated, and the supernatant is the golden ear fermentation liquid.

[0035] Preferably, the extraction temperature is 80-90°C and the extraction time is 40-50 min; further preferably, the extraction temperature is 85°C and the extraction time is 40 min.

[0036] Preferably, the sterilization temperature is 120-125°C and the time is 12-17 min; further preferably, the sterilization temperature is 121°C and the time is 15 min.

[0037] Preferably, the fermentation temperature is 35-40°C and the fermentation time is 48-72h; further preferably, the fermentation temperature is 37°C and the fermentation time is 72h.

[0038] In another aspect, the present invention provides a method for preparing the above-mentioned Dendrobium officinale fermentation liquid composition, which comprises mixing the Dendrobium officinale fermentation liquid and Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid.

[0039] In another aspect, the present invention provides the use of the above-mentioned fermented product of Dendrobium officinale in blood sugar-lowering products.

[0040] Compared with the prior art, the present invention has the following advantages:

[0041] 1 The present invention provides a Dendrobium officinale fermentation liquid composition, comprising Dendrobium officinale fermentation liquid and Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid, and the applicant has found that when the volume ratio of Dendrobium officinale fermentation liquid to Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid is 1: (0.5-2), it has a good α-glucosidase inhibition rate.

[0042] 2. The present invention provides a Dendrobium officinale fermentation liquid composition, which has a better α-glucosidase inhibition rate when the volume of the Dendrobium officinale fermentation liquid and the Tremella fuciformis fermentation liquid or the Ganoderma lucidum fermentation liquid is 1: (1-2), and can be applied to blood sugar lowering products. DETAILED DESCRIPTION

[0043] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0044] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0045] The present invention is further described below by way of specific examples. If no specific experimental steps or conditions are specified in the examples, the conventional steps or conditions described in the literature in the art can be used. All reagents without manufacturer specified can be obtained through conventional commercial channels.

[0046] In the following examples, the source information of some products is as follows:

[0047] Dendrobium officinale and Tremella fuciformis were from Zhejiang Fangge Pharmaceutical Co., Ltd. of Hu Qingyu Tang Group; Ganoderma lucidum was from Fujian Xianzhilou Biotechnology Group; cellulase was purchased from Xiasheng (Beijing) Biotechnology Development Co., Ltd. with an enzyme activity of 3500 U / g, pectinase was purchased from Henan Zhongchen Biotechnology Co., Ltd. with an enzyme activity of 20,000 U / g, and papain was purchased from Nanning Shanwan Biotechnology Co., Ltd. with an enzyme activity of 100,000 U / g.

[0048] Lactobacillus acidophilus was purchased from China General Microorganism Culture Collection Center with a collection number of CGMCC1.12735; Lactobacillus plantarum with a collection number of ACCC 11016 was purchased from China Agricultural Microorganism Culture Collection Management Center; Lactobacillus plantarum with a collection number of CGMCC1.1856 was purchased from China General Microorganism Culture Collection Center.

[0049] Preparation Example 1 Preparation of fermentation bacteria

[0050] Take 52.2 g of MRS broth, add 1000 mL of distilled water, stir and heat to boil until completely dissolved, divide into portions, sterilize under high pressure at 121°C for 15 min, and set aside.

[0051] The lactic acid bacteria strains (Lactobacillus acidophilus with a preservation number of CGMCC1.12735, Lactobacillus plantarum with a preservation number of ACCC 11016, and Lactobacillus plantarum with a preservation number of CGMCC1.1856) stored in a -80°C refrigerator were taken out and inoculated into MRS medium at a 1% inoculum volume. The culture was cultured at 37°C for 24 h. The activation method was repeated three times until the viable cell count was 1.0×10 8 CFU / mL and above.

[0052] MRS broth was purchased from Hangzhou Best Biotechnology Co., Ltd., and its culture medium formula was 10.0 g / L peptone, 5.0 g / L beef extract powder, 4.0 g / L yeast extract powder, 20.0 g / L glucose, 2.0 g / L dipotassium hydrogen phosphate, 2 g / L triammonium hydrogen citrate, 5.0 g / L sodium acetate, 0.2 g / L magnesium sulfate, 0.05 g / L manganese sulfate, and 1 g / L Tween-80.

[0053] Preparation Example 2 Preparation of Ganoderma lucidum fermentation liquid

[0054] The fruiting bodies of Ganoderma lucidum were dried and crushed, and Ganoderma lucidum and water were mixed evenly at a solid-liquid ratio of 1:20 (g:mL) and then pulped to obtain Ganoderma lucidum slurry. A composite enzyme solution (papain: cellulase: pectinase = 1:2:2) was added to the Ganoderma lucidum slurry and mixed evenly. The enzymatic hydrolysis conditions were 0.8% composite enzyme dosage, enzyme solution pH 4.5, and enzymatic hydrolysis at 150r / min for 3h in a 50℃ constant temperature shaker to obtain Ganoderma lucidum enzymatic hydrolyzate. The enzyme was inactivated in a 90℃ water bath for 10 min, cooled and centrifuged at 5000×g for 10 min, and the supernatant was taken for fermentation. The activated Lactobacillus acidophilus with a preservation number of CGMCC1.12735 was inoculated into the supernatant at an inoculation rate of 2%, cultured at 37℃ for 60h, and the supernatant was centrifuged and pasteurized to obtain Ganoderma lucidum fermentation liquid.

[0055] Preparation Example 3 Preparation of Dendrobium officinale fermentation liquid

[0056] Take the stems of Dendrobium officinale, dry them, crush them, and pass them through a 60-mesh sieve. Mix them with pure water at a solid-liquid ratio of 1:15 (g:mL). The enzymatic hydrolysis conditions are 4% cellulase addition (based on the mass of Dendrobium officinale), enzymatic hydrolysis temperature of 50℃, and enzymatic hydrolysis for 4h. After the enzymatic hydrolysis is completed, inactivate them in a 90℃ water bath for 10 min, cool them, and obtain the enzymatic hydrolyzate of Dendrobium officinale.

[0057] The activated Lactobacillus plantarum culture liquid with the preservation number of ACCC 11016 was inoculated into the enzymatic hydrolyzed liquid of Dendrobium officinale at a 2% inoculation rate, and the fermentation was carried out at a constant temperature of 37°C for 48 hours and a fermentation speed of 150 r / min to obtain the fermented Dendrobium officinale juice, which was then pasteurized. Finally, the supernatant was taken as the fermented liquid of Dendrobium officinale at 4800 r / min for 10 minutes.

[0058] Preparation Example 4 Preparation of Tremella Fermentation Broth

[0059] Fresh golden ear was cut into small pieces and mixed with pure water at a solid-liquid ratio of 1:50 (g:mL), extracted at 85 °C for 40 min, and cooled after extraction to obtain golden ear water extract.

[0060] The Tremella fuciformis water extract was sterilized at 121°C for 15 minutes, then inoculated with 2% activated Lactobacillus plantarum culture with a preservation number of CGMCC1.1856, and fermented at 37°C for 72 hours. After the fermentation, the Tremella fuciformis fermented liquid was obtained, and then pasteurized. Finally, centrifuged at 5000×g for 15 minutes, and the supernatant was taken, which was the Tremella fuciformis fermented liquid.

[0061] Example

[0062] This embodiment provides a Dendrobium officinale fermentation liquid composition, which is obtained by mixing the Dendrobium officinale fermentation liquid obtained in Preparation Example 3, the Ganoderma lucidum fermentation liquid obtained in Preparation Example 2, or the Tremella fuciformis fermentation liquid obtained in Preparation Example 4 in a certain proportion. The specific composition and volume ratio are shown in Table 1.

[0063] Comparative Example

[0064] This comparative example provides a composition, which is obtained by mixing the Ganoderma lucidum fermentation liquid obtained in Preparation Example 2 and the Tremella fuciformis fermentation liquid obtained in Preparation Example 4 in a certain proportion. The specific composition and volume ratio are shown in Table 1.

[0065] Table 1 Composition and proportion of Dendrobium officinale fermentation liquid composition

[0066]

[0067] Experimental Example 1 α-glucosidase solution determination

[0068] Samples to be tested: the fermented Dendrobium officinale liquid obtained in Preparation Example 3, the fermented Ganoderma lucidum liquid obtained in Preparation Example 2, the fermented Tremella fuciformis liquid obtained in Preparation Example 4, and the compositions obtained in Examples 1-6 and Comparative Examples 1-3.

[0069] (1) Reagent preparation

[0070] 0.1 mol / L Na2CO3: Weigh 2.1198 g of Na2CO3 and dissolve it in 200 mL of distilled water.

[0071] 25mM pH=6.9: Weigh 4.477g of disodium hydrogen phosphate dodecahydrate and 1.95g of sodium dihydrogen phosphate dihydrate, dissolve in 500mL of distilled water respectively, mix well and adjust the pH to 6.9.

[0072] α-glucosidase solution with an activity of 0.1U: first prepare 1mg / mL enzyme solution (50U / mg), then gradually dilute and store at low temperature.

[0073] 5mmol / mL PNPG solution: weigh 0.151g of PNPG and dissolve it in 100mL PBS and mix well.

[0074] (2) Experimental process

[0075] Mix 0.2 mL of sample with 1 mL of α-glucosidase solution (enzyme activity is 0.1 U) in a 37°C water bath for 10 min, then add 0.5 mL of 5 mmol / mL PNPG solution and mix in a 37°C water bath for 10 min, then add 0.1 mol / l Na2CO3 solution to terminate the reaction, and measure the absorbance at 405 nm. The total system is 2.7 mL. Acarbose is used as a positive control. The clearance rate is calculated according to the following formula: Inhibition rate (%) = [1-(A4-A3) / (A2-A1)] × 100%

[0076] Where A4: absorbance of the experimental group; A3: absorbance of the experimental background group (PBS replaces the enzyme solution); A2: absorbance of the blank group (PBS replaces the sample); A1: absorbance of the blank background group (PBS replaces the sample and enzyme solution)

[0077] See Table 2 for specific results.

[0078] Table 2 α-glucosidase inhibition rate of samples

[0079]

[0080] Note: Different letters indicate significant differences among different groups

[0081] As can be seen from Table 2, the Tremella fuciformis fermented liquid obtained in Preparation Example 4 has the lowest α-glucosidase inhibition rate, but when the Tremella fuciformis fermented liquid and the Dendrobium officinale fermented liquid are in a ratio of (0.5-2):1, the α-glucosidase inhibition rate is higher than that of the Tremella fuciformis fermented liquid; when the Tremella fuciformis fermented liquid and the Dendrobium officinale fermented liquid are in a ratio of 1:1 or 2:1, the α-glucosidase inhibition rate is higher than that of the Tremella fuciformis fermented liquid and the Dendrobium officinale fermented liquid, and is significant, indicating that the Tremella fuciformis fermented liquid and the Dendrobium officinale fermented liquid have a better α-glucosidase inhibition rate in a specific ratio.

[0082] When the ratio of Ganoderma lucidum fermented liquid to Dendrobium officinale fermented liquid was 1:1 or 2:1, the α-glucosidase inhibition rate was higher than that of Ganoderma lucidum fermented liquid and Dendrobium officinale fermented liquid, and the rate was significant. This indicates that Ganoderma lucidum fermented liquid and Dendrobium officinale fermented liquid have a better α-glucosidase inhibition rate synergistically at a specific ratio.

[0083] The α-glucosidase inhibition rate of the composition prepared from Ganoderma lucidum fermentation liquid and Tremella fuciformis fermentation liquid is lower than that of the α-glucosidase inhibition rate of the Ganoderma lucidum fermentation liquid and Tremella fuciformis fermentation liquid alone, and the inhibition rate is significant, indicating that not all combinations of raw materials that have the ability to inhibit α-glucosidase activity have the effect of synergistically enhancing the α-glucosidase rate.

[0084] α-glucosidase located in the small intestinal epithelial mucosa is a key enzyme involved in carbohydrate metabolism. α-glucosidase inhibitors can slow down carbohydrate metabolism by inhibiting the activity of α-glucosidase, thereby controlling the rate of blood sugar production. Currently, α-glucosidase inhibitors have been widely used in hypoglycemic treatment, so the present invention uses α-glucosidase inhibition rate to verify the hypoglycemic effect of the composition. In the present invention, when the ratio of Dendrobium officinale fermentation liquid to Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid is 1: (1-2), it has a good α-glucosidase inhibition rate, indicating that the Dendrobium officinale fermentation liquid composition provided by the present invention can be applied to hypoglycemic products.

[0085] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A Dendrobium officinale fermented liquid composition having blood sugar lowering effect, characterized in that: It comprises a fermented liquid of Dendrobium officinale and a fermented liquid of Tremella fuciformis or a fermented liquid of Dendrobium officinale and a fermented liquid of Ganoderma lucidum, wherein the volume ratio of the fermented liquid of Dendrobium officinale to the fermented liquid of Tremella fuciformis or the fermented liquid of Ganoderma lucidum is 1:(0.5-2).

2. The composition according to claim 1, characterized in that The volume ratio of the Dendrobium officinale fermentation liquid and Tremella fuciformis fermentation liquid or Ganoderma lucidum fermentation liquid is 1:(1-2).

3. The composition according to claim 1, characterized in that The Dendrobium officinale fermented liquid is prepared by mixing Dendrobium officinale and water, performing enzymatic hydrolysis, and inoculating plant lactobacillus with a preservation number of ACCC 11016 for fermentation.

4. The composition according to claim 1, characterized in that The ganoderma lucidum fermented liquid is prepared by inoculating the supernatant obtained by mixing ganoderma lucidum and water, performing enzymolysis, enzyme inactivation and solid-liquid separation, and fermenting with Lactobacillus acidophilus with a preservation number of CGMCC1.12735.

5. The composition according to claim 1, characterized in that The Tremella fuciformis fermented liquid is prepared by extracting Tremella fuciformis and water and inoculating plant lactobacillus with a preservation number of CGMCC1.1856 for fermentation.

6. The composition according to claim 3, characterized in that The enzyme preparation selected for enzymatic hydrolysis of Dendrobium officinale is cellulase, and the addition amount of the cellulase is 3-5%.

7. The composition according to claim 4, characterized in that The enzyme preparations used for enzymolysis of Ganoderma lucidum include papain, cellulase and pectinase; the added amount of the enzyme preparations is 0.5-1.0%.

8. A composition according to any one of claims 3 to 7, characterized in that: The fermentation temperature is 35-40°C and the fermentation time is 48-72h.

9. The method for preparing the composition according to any one of claims 1 to 8, characterized in that: Just mix the Dendrobium officinale fermentation liquid and the Tremella fuciformis fermentation liquid or the Ganoderma lucidum fermentation liquid evenly.

10. Use of the Dendrobium officinale fermented liquid composition according to any one of claims 1 to 8 in the preparation of a blood sugar-lowering product.

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