Kudzuvine root and hovenia dulcis thunb plant beverage as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and relates to a radix puerariae and hovenia dulcis thunb plant beverage, a preparation method and application, the radix puerariae and hovenia dulcis thunb plant beverage comprises the following raw materials: radix puerariae, hovenia dulcis thunb, poria cocos, pericarpium citri reticulatae, turmeric, rhizoma phragmitis and mulberry according to the mass ratio of (1-10): (1-10): (0.5-5): (0.5-6): (0.01-1): (0.1-5): (0.1-5). The radix puerariae, hovenia dulcis thunb, pericarpium citri reticulatae, poria cocos, turmeric, rhizoma phragmitis and mulberries are used as raw materials and are reasonably combined, so that the alcohol effect is improved, and meanwhile, the damage of alcohol to internal organs such as spleen, stomach, liver and gall can be prevented; medicinal and edible raw materials are adopted, so that higher safety is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines and relates to a kudzu root and hovenia dulcis plant beverage, a preparation method and an application thereof. Background Art

[0002] Alcohol abuse can lead to serious personal and social problems, including various physical illnesses, mental health problems, family problems and social problems; alleviating the harm caused by alcohol and finding alcohol detoxification drugs have received widespread attention.

[0003] Common alcohol sobering drugs can be divided into gastric mucosal protective drugs, diuretics, liver protective drugs, metabolic drugs and emergency drugs. 1. Gastric mucosal protective drugs: such as bismuth potassium citrate capsules, colloidal bismuth pectin capsules and other bismuth agents, which can form a protective layer on the gastric mucosa, reduce the irritation and damage of alcohol to the gastric mucosa, thereby relieving stomach discomfort after drinking, and have a certain alcohol sobering effect; 2. Diuretics: such as hydrochlorothiazide tablets, furosemide tablets, etc., by increasing the filtration and absorption of kidney function, alcohol is quickly excreted from the body, thereby achieving the purpose of sobering up; but patients with hypertension, diabetes, hyperuricemia and other groups need to use it with caution; 3. Liver protective drugs: such as compound glycyrrhizin capsules, metadoxine, etc., can protect liver cells, reduce alcohol and metabolites The damage caused by alcohol, thereby reducing the liver inflammation caused by alcohol, protecting the liver, and maintaining the liver's alcohol-resolving function; 4. Metabolic drugs: Common ones include vitamin C tablets and vitamin B tablets. Vitamin C can promote the metabolism of alcohol in the body, and B vitamins can promote the decomposition of alcohol. At the same time, they nourish the nerves and reduce the damage of alcohol to nerve cells, thereby achieving the effect of alcohol relief; 5. First aid medicine: mainly used for acute alcohol poisoning, such as ancacaine injection, naloxone hydrochloride injection, etc., which can inhibit the excitatory effect of alcohol on the nervous system, speed up blood circulation, and promote body metabolism, thereby achieving the effect of alcohol relief.

[0004] Since the liver is the main organ responsible for digesting and metabolizing alcohol, and alcohol can damage liver cells, liver-protecting and alcohol-detoxifying drugs can enhance the liver's metabolic function, promote the decomposition and excretion of alcohol, reduce the burden on the liver, and protect liver cells from oxidative damage; they are currently a more popular type of alcohol-detoxifying drugs. Although liver-protecting and alcohol-detoxifying drugs have the effect of detoxifying alcohol, they cannot completely eliminate the damage caused by alcohol to the body. After drinking, the spleen, stomach, liver, gallbladder and other internal organs of the human body are still damaged. In addition, existing alcohol-detoxifying drugs will have side effects on the human body. Summary of the invention

[0005] In order to solve the technical problem that the existing alcohol detoxification drugs cannot completely eliminate the harm of alcohol to the body and may produce side effects on the human body, the present invention provides a kudzu root and Hovenia dulcis plant beverage.

[0006] The invention uses kudzu root, Hovenia dulcis, dried orange peel, Poria, turmeric, reed root and mulberry as raw materials, and according to reasonable compatibility, can improve the hangover effect while preventing alcohol from damaging internal organs such as the spleen, stomach, liver and gallbladder; the use of raw materials that are both medicinal and edible is safer.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A kudzu root and hovenia dulcis plant beverage is composed of the following raw materials in the following mass ratios: kudzu root, hovenia dulcis fruit, tuckahoe, tangerine peel, turmeric, reed root and mulberry (1-10): (1-10): (0.5-5): (0.5-6): (0.01-1): (0.1-5): (0.1-5).

[0009] A kudzu root and hovenia dulcis plant beverage is composed of the following raw materials in the following mass ratio: kudzu root, hovenia dulcis fruit, tuckahoe, tangerine peel, turmeric, reed root and mulberry in the ratio of 3:3:2:2:0.5:1.5:1.5.

[0010] The preparation method of the kudzu root and Hovenia dulcis plant beverage comprises the following steps:

[0011] S1. Prepare the raw materials according to the above mass ratio: Pueraria root, Hovenia dulcis, Poria cocos, dried orange peel, turmeric, Phragmites australis and mulberry;

[0012] S2. For each of the above raw materials, a decoction method is used to obtain an extract;

[0013] S3, concentrating the extract under normal pressure to obtain an extract solution;

[0014] S4. Add sucralose and xylitol to the extract, mix evenly, filter, and then obtain the filtrate through filling, sterilization, and cooling to obtain the Pueraria lobata and Hovenia dulcis plant beverage; add 2g-10g of xylitol and 1mg-3mg of sucralose to every 100ml of the extract.

[0015] Further defined, the specific process of step S2 is:

[0016] Mix all the raw materials, then soak them in water for 30 minutes, then add water again, perform the first extraction at 100°C, and collect the first filtrate and the first residue;

[0017] Continue to add water to the primary filter residue, perform a second extraction at 100°C, and collect the secondary filtrate and secondary filter residue;

[0018] Continue to add water to the secondary filter residue, perform a third extraction at 100°C, collect the three filtrates, and discard the three filter residues; combine the obtained first filtrate, second filtrate and third filtrate to obtain an extract.

[0019] It is further defined that in step S3, during each extraction, the mass ratio of solid material to water is 1:12; the solid material is a raw material or a filter residue; and the extraction time for each extraction is 1.5 hours.

[0020] It is further defined that in step S3, the atmospheric pressure concentration is carried out at 100° C. and is concentrated until 100 ml of the extract contains 27 g of the raw material.

[0021] An application of the Pueraria lobata and Hovenia dulcis plant beverage in sobering up.

[0022] The kudzu root and Hovenia dulcis plant beverage is used in sobering up by prolonging the latent period of drunkenness and shortening the sobering time.

[0023] Traditional Chinese medicine believes that alcohol is a product that is damp, hot and toxic. Drinking a small amount of alcohol can dispel cold, relax muscles and activate collaterals, but drinking too much alcohol can damage the spleen, stomach, liver and gallbladder and other internal organs. Therefore, the present invention provides a kudzu root and tangerine peel plant beverage, which is made from 7 raw materials of kudzu root, tangerine peel, tuckahoe, turmeric, reed root and mulberry, which are both medicinal and edible. In the prescription, tangerine peel and pueraria detoxify alcohol, while tangerine peel is diuretic and pueraria raises yang. While detoxifying alcohol, it takes into account the characteristics of dampness and easy to trap the spleen, promotes the clear qi of the spleen, and promotes dampness and heat to descend, which are all monarch drugs. In the prescription, tuckahoe, turmeric and tangerine peel all enter the spleen meridian, can help the monarch drug to strengthen the spleen and remove dampness, and help the spleen to move healthily, and are all minister drugs. Mulberry protects the liver, while reed root clears heat and nourishes yin, promotes the production of body fluid and quenches thirst, and is used to improve thirst and vomiting caused by alcohol heat and body fluid damage. The two herbs are used together as adjuvants. The whole formula is reasonably matched, and it can work together to detoxify alcohol, strengthen the spleen and remove dampness, protect the liver and nourish the stomach, clear heat and nourish yin, and closely follow the etiology and pathogenesis of alcoholic liver damage to cure the disease.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The present invention conducts in-depth research on traditional Chinese medicine, and applies Pueraria root, Hovenia dulcis, dried orange peel, Poria cocos, turmeric, Phragmites australis and mulberry to alcohol relieving products. These ingredients can reduce the concentration of alcohol in the blood, relieve the drowsiness caused by alcohol, and prevent alcohol poisoning.

[0026] 2. The kudzu root and Hovenia dulcis plant beverage of the present invention uses kudzu root and Hovenia dulcis as main medicines. These medicinal materials contain natural flavonoid components, which have the functions of protecting the brain from inhibiting nerve receptors, reducing the alcohol concentration in the blood, and alleviating the drowsiness caused by alcohol.

[0027] 3. The raw materials used in the present invention are medicinal materials that are both medicinal and edible, have no side effects on the human body, are green and safe, and can be used by different groups of people.

[0028] 4. The present invention adopts methods such as decoction extraction and concentration to prepare the Pueraria lobata and Hovenia dulcis plant beverage, which not only has the effect of sobering up, but also can meet the diverse needs of consumers. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is the in vitro activation rate of alcohol dehydrogenase in each group;

[0030] Figure 2 The results of comparison of the intoxication latency of mice in each group;

[0031] Figure 3 Comparison results of sobering-up time of mice in each group;

[0032] Figure 4 The results of each group's effect on the mouse liver coefficient;

[0033] Figure 5 The results of each group's effect on the activity of alcohol dehydrogenase in mouse liver;

[0034] Figure 6 The results of each group's effect on the activity of acetaldehyde dehydrogenase in mouse liver;

[0035] Figure 7 The results of each group's effect on malondialdehyde (MDA) in mouse liver;

[0036] Figure 8 These are the effects of each group on reduced glutathione (GSH) in mouse liver. DETAILED DESCRIPTION

[0037] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0038] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0039] Technologies, methods, and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered as part of the specification.

[0040] It should also be understood that the specific embodiments described above are only used to explain the present invention, and the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, can make equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, which should be covered by the protection scope of the present invention / invention.

[0041] The invention provides a kudzu root and hovenia dulcis plant beverage, which is composed of the following raw materials in the following mass ratio: kudzu root, hovenia dulcis fruit, tuckahoe, tangerine peel, turmeric, reed root and mulberry (1-10): (1-10): (0.5-5): (0.5-6): (0.01-1): (0.1-5): (0.1-5).

[0042] Specifically, the Pueraria lobata and Hovenia dulcis plant beverage is composed of the following raw materials in the following mass ratios: Pueraria lobata, Hovenia dulcis, Poria cocos, dried tangerine peel, turmeric, reed root and mulberry in the ratio of 3:3:2:2:0.5:1.5:1.5.

[0043] The present invention also provides a method for preparing a Pueraria lobata and Hovenia dulcis plant beverage, comprising the following steps:

[0044] S1. Prepare the raw materials according to the above mass ratio: Pueraria root, Hovenia dulcis, Poria cocos, dried orange peel, turmeric, reed root and mulberry.

[0045] S2. For each of the above raw materials, an extract is obtained by decoction.

[0046] The specific process of step S2 of the present invention is:

[0047] Mix all the raw materials, then soak them in water for 30 minutes, then add water again, perform the first extraction at 100°C, and collect the first filtrate and the first residue;

[0048] Continue to add water to the primary filter residue, perform a second extraction at 100°C, and collect the secondary filtrate and secondary filter residue;

[0049] Continue to add water to the secondary filter residue, perform a third extraction at 100°C, collect the three filtrates, and discard the three filter residues; combine the obtained first filtrate, second filtrate and third filtrate to obtain an extract.

[0050] Preferably, during each extraction, the mass ratio of solid material to water is 1:12; the solid material is raw material or filter residue; and the extraction time for each extraction is 1.5 hours.

[0051] S3. The extract is concentrated under normal pressure to obtain an extract solution; the normal pressure concentration is carried out at 100° C. and is concentrated until 100 ml of the extract solution contains 27 g of the raw material.

[0052] S4. Add sucralose and xylitol to the extract, mix evenly, filter, and then obtain the filtrate through filling, sterilization, and cooling to obtain the Pueraria lobata and Hovenia dulcis plant beverage; add 2g-10g of xylitol and 1mg-3mg of sucralose to every 100ml of the extract.

[0053] Xylitol and sucralose are flavoring agents to enhance the taste of the Pueraria lobata and Hovenia dulcis plant beverage and meet the needs of different groups of people.

[0054] The kudzu root and tangerine peel plant beverage provided by the present invention has kudzu root and tangerine peel as monarch drugs, tuckahoe, dried orange peel and turmeric as ministerial drugs, and reed root and mulberry as adjuvant drugs; the monarch, ministerial, adjuvant and adjuvant drugs are reasonably matched, and the whole formula is used together to play the functions of detoxifying alcohol, strengthening the spleen and removing dampness, protecting the liver and nourishing the stomach, and clearing away heat and nourishing yin, closely following the etiology and pathogenesis of alcoholic liver injury, and treating the disease from the root. Therefore, the kudzu root and tangerine peel plant beverage can prolong the incubation period of drunkenness, shorten the sobering time, promote the decomposition and metabolism of acetaldehyde in the liver, have a good detoxifying effect, and can protect the health of the human body and avoid the harm caused by alcohol.

[0055] The effects of the Pueraria lobata and Hovenia dulcis plant beverage of the present invention are described in detail with reference to specific examples.

[0056] It should be noted that, unless otherwise specified, the drugs, reagents, etc. used in the following examples are all conventional and known products purchased from the market.

[0057] It should be noted that, unless otherwise specified, the operations used in the following embodiments are all conventional operations. For example, if there is no limitation on the pressure and temperature of the operation, the operations are all performed at normal pressure and temperature.

[0058] Example 1

[0059] This embodiment provides a kudzu root and hovenia dulcis plant beverage composed of the following raw materials in mass: 3g kudzu root, 3g hovenia dulcis, 2g tuckahoe, 2g tangerine peel, 0.5g turmeric, 1.5g reed root, and 1.5g mulberry.

[0060] In this embodiment, the preparation method of the Pueraria lobata and Hovenia dulcis plant beverage comprises the following steps:

[0061] S1. Take the above-mentioned raw materials of various weights, soak in water for 30 minutes, and boil for 3 times.

[0062] Specifically, purified water was added to the above-mentioned raw materials in a mass ratio of 1:12, and the mixture was soaked for 30 minutes, then extracted at 100°C for 1.5 hours, and the filtrate was collected; purified water was added to the filter residue in a mass ratio of 1:12, and a second extraction was performed at 100°C, and the extraction was also performed for 1.5 hours, and the filtrate was collected; purified water was added to the filter residue in a mass ratio of 1:12, and a third extraction was performed at 100°C, and the extraction was also performed for 1.5 hours, and the filtrate was collected, the filter residue was discarded, and the three filtrates were combined to obtain an extract.

[0063] S2. Concentrating the extract under normal pressure to obtain an extract solution; the concentration is to concentrate the extract under normal pressure and a temperature of 100° C. until the concentration of the concentrate is equivalent to 27 g / 100 ml of the original medicinal material, thereby obtaining an extract solution; that is, the extract solution is concentrated until 100 ml of the extract solution contains 27 g of the raw material.

[0064] S3, add sucralose and xylitol to the extract, mix evenly, filter, take the filtrate, fill, sterilize, and cool to obtain the Pueraria lobata and Hovenia dulcis plant beverage. Add 6g xylitol and 2mg sucralose to every 100ml of the extract.

[0065] In the above embodiment 1, the mass ratio of Pueraria root, Hovenia dulcis, Poria cocos, dried orange peel, Curcuma longa, Phragmites australis and mulberry can also be replaced within the following range (1-10): (1-10): (0.5-5): (0.5-6): (0.01-1): (0.1-5): (0.1-5).

[0066] The sobering performance of the Pueraria root and Hovenia dulcis plant beverage prepared in the above embodiment was studied. In order to illustrate the advantages of the solution of the present invention, the following comparative example was designed.

[0067] Comparative Example 1

[0068] The kudzu root and Hovenia dulcis plant beverage provided in this comparative example is composed of the following raw materials in terms of mass: 3g of kudzu root and 3g of Hovenia dulcis.

[0069] Comparative Example 2

[0070] This embodiment provides a kudzu root and Hovenia dulcis plant beverage composed of the following raw materials in mass: 3g kudzu root, 3g Hovenia dulcis, 2g Poria, 2g dried orange peel, and 0.5g turmeric.

[0071] The alcohol-detoxifying effect was verified by in vitro alcohol-detoxifying activity test and in vivo animal alcohol-detoxifying test.

[0072] 1. In vitro alcohol sobering activity test

[0073] The improved Valle & Hoch method was used to measure the activities of alcohol dehydrogenase in Example 1, Comparative Example 1, Comparative Example 2 and the positive control drug (Metadoxine).

[0074] The test method is: 1.5mL 32mmol / L sodium pyrophosphate buffer (pH=8.8), 1.0mL 27mmol / L NAD+ (oxidized coenzyme I) solution, 0.5mL 11.5% (v / v) ethanol, 0.1mL 30mg / mL sample are mixed, incubated at 25℃ for 5min, and then 0.1mL ADH (0.64μg / mL) solution is immediately added. The control group uses 0.1mL distilled water instead of sample solution, and the rest of the operation is the same. Immediately read the value every 10s at a wavelength of 340nm, and measure continuously for 5min. The method for calculating the activity of alcohol dehydrogenase is to plot A against time and calculate A 340nmThe increase in / min was calculated based on the molar extinction coefficient of NADH (nicotinamide adenine dinucleotide) at 340nm of 6.22, i.e., the in vitro ADH activity. The ADH activity was expressed in nanomoles of NADH generated per minute.

[0075]

[0076] Where: V is the volume of the total reaction solution (mL); DF is the dilution factor; 6.22 is the millimolar extinction coefficient of NADH at 340nm; 0.1 is the volume of the enzyme solution (mL); ADH activity 样品 is the ADH activity value of the sample; ADH activity control is the ADH activity value of the control group; A 340nm / min is the absorbance change per minute.

[0077] The test results are as follows Figure 1 Note: the monarch drug group is comparative example 1, the monarch + minister drug group is comparative example 2, and the monarch + minister + assistant drug group is example 1.

[0078] from Figure 1 It can be seen that the in vitro activation rate of alcohol dehydrogenase in each group is as follows: Example 1> Metadoxine> Comparative Example 2> Comparative Example 1, indicating that after the reasonable combination of the monarch, minister, assistant and envoy of the present invention, the in vitro activation rate of alcohol dehydrogenase is significantly improved. Therefore, compared with the existing alcohol detoxification drugs, the Pueraria root and Hovenia dulcis plant beverage provided by the present invention has a faster alcohol metabolism and a good alcohol detoxification effect.

[0079] 2. Animal in vivo alcohol detoxification test

[0080] 2.1. Sample solution preparation

[0081] The mass of each raw material in the kudzu root and Hovenia dulcis plant beverage provided in Example 1 was increased by 6 times, and the kudzu root and Hovenia dulcis plant beverage was prepared by the method of Example 1 as a sample solution.

[0082] Directly take 50 ml as the high-dose group of sample solution; take 50 ml and dilute it to 100 ml with purified water as the medium-dose group of sample solution; take 50 ml of the medium-dose group of sample solution and dilute it to 100 ml with purified water as the low-dose group of sample solution.

[0083] Take one Metadoxine tablet (0.5 g) and dissolve it in 50 ml of purified water.

[0084] 2.2. Experimental Animal Grouping and Dosing

[0085] SPF KM mice, 18-22g, male, 160 mice, certificate number 20230629Ab220600000. Adaptive feeding for 7 days, normal water, random grouping according to body weight, divided into 16 groups, 10 mice in each group. 8 groups were used for animal behavior experiments, and the other 8 groups were used for animal experimental index detection.

[0086] The groups and dosing methods are shown in Table 1.

[0087] Table 1 Grouping and administration method

[0088]

[0089] 2.3 Animal Behavior

[0090] The righting reflex experiment is used to study animal behavior.

[0091] After the mice were treated according to the dosing method in Table 1, the appearance and behavior of the mice in each group were observed. The mice that were not drunk were flexible and active; the drunk mice showed changes in their bodies, changed gait, unstable crawling, crawling with their hind abdomen dragging on the ground, reduced activity, messy hair, and loss of righting reflex.

[0092] To judge whether a mouse is drunk, the main criterion is whether the righting reflex disappears: the mouse that has been gavaged with alcohol is laid flat on its back. If it is drunk, the righting reflex disappears, and the criterion is that the mouse can maintain the back-down posture for more than 30 seconds; if the mouse maintains the back-down posture for less than 30 seconds, it is considered that the righting reflex still exists. The recovery of the righting reflex is based on the mouse's free movement, flexibility, spirit, smooth hair, and inability to maintain the back-down posture for more than 30 seconds. The calculation formula for the incubation period of drunkenness and the sobering time is as follows:

[0093] Latency of intoxication = time of disappearance of righting reflex - time of intragastric administration of alcohol (2-1)

[0094] Sobering up time = righting reflex recovery time - intragastric administration time (2-2)

[0095] The above results of the mouse drunkenness latency and mouse sobering time are as follows Figure 2 and Figure 3 In the figure: *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0096] Depend on Figure 2As shown, there was no significant difference in the incubation period of drunkenness in mice between the low-dose group of metadoxine and the low-dose group of sample solution and the model group (P>0.05). However, with the increase of the dose of the sample administered orally, the incubation period of drunkenness in the mice of the medium-dose group and the high-dose group of metadoxine was significantly higher than that of the mice of the model group (P<0.01), indicating that it began to play a certain role in delaying the intoxication of mice. The incubation period of drunkenness in the medium-dose group of the sample solution was also higher than that of the model group (P<0.05), and the incubation period of drunkenness in the high-dose group of mice was significantly higher than that of the model group, with a very significant difference (P<0.01).

[0097] Depend on Figure 3 As shown, there was no significant difference in the sobering time of mice between the low-dose group of metadoxine and the low-dose group of sample solution and the model group (P>0.05). However, with the increase of the dose of the sample administered intragastricly, the intoxication latency of mice in the medium-dose group and the high-dose group of metadoxine and the sample solution was significantly higher than that of mice in the model group, with a very significant difference (P<0.01).

[0098] 2.4、 Animal experiment index detection

[0099] The mice were treated according to the administration method in Table 1 and then killed by cervical dislocation 1 hour later, and the liver was removed.

[0100] 2.4.1 Liver coefficient

[0101] Before the mouse was dissected, the abdominal skin was disinfected with 75% alcohol, and the abdominal skin was cut open to expose the abdominal cavity. The liver was carefully separated with ophthalmic forceps and surgical scissors, and the liver was rinsed with saline, dried with filter paper, and weighed. The temperature was kept low throughout the dissection process. The liver coefficient was expressed as the ratio of liver weight (g) to body weight (g), and the calculation formula was:

[0102]

[0103] 2.4.2 Preparation of liver homogenate

[0104] After weighing the liver, accurately weigh 0.5g of the liver and put it into a 10mL centrifuge tube. According to the ratio of weight (g): volume (mL) = 1:9, add 9 times the volume of normal saline, and use a low-temperature homogenizer to homogenize the liver tissue. The resulting homogenate is centrifuged at 2500r / min for 10min at 4°C. The supernatant is stored in a 10mL disposable centrifuge tube, which is a 10% liver homogenate, and is stored in a -80°C refrigerator for testing; the temperature of the liver homogenate preparation process is preferably maintained at 2-8°C throughout.

[0105] 2.4.3 Biochemical indicators tested

[0106] Alcohol dehydrogenase, acetaldehyde dehydrogenase, malondialdehyde, reduced glutathione.

[0107] 2.4.4 Methods for detecting biochemical indicators

[0108] Alcohol dehydrogenase determination: ultraviolet spectrophotometry; acetaldehyde dehydrogenase determination: ultraviolet spectrophotometry; malondialdehyde determination: TBA colorimetry; reduced glutathione determination: colorimetry.

[0109] The detection methods of all the above indicators were strictly carried out in accordance with the instructions of each kit.

[0110] 2.4.5 Statistical processing methods

[0111] SPSS Statistics 26.0 software was used for data analysis. One-way ANOVA was used for significant comparisons among multiple groups, and SNK and LSD methods were used for significant comparisons between two groups. GraphPad Prism 10.1.2 software was used to draw statistical graphs. P < 0.05 indicated statistical differences, and P < 0.01 indicated extremely significant differences.

[0112] 2.4.6 Results of alcohol detoxification in mice

[0113] 2.4.6.1. Effects on mouse liver coefficient

[0114] The results of the effects of each group on the liver coefficient of mice are as follows Figure 4 And as shown in Table 2.

[0115] Among them: s: compared with the blank control group, P < 0.05; ss: compared with the blank control group, P < 0.01.

[0116] Among them: *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0117] See also Figure 4 As shown in Table 2, in the changes of liver coefficient of mice, there was a significant difference in liver coefficient between the model group and the blank control group (P < 0.01), while there was no significant difference in liver coefficient between the low-dose metadoxine group, the medium-dose metadoxine group and the low-dose sample solution group and the model group (P > 0.05). However, with the increase of the dose of the oral intervention substance, the liver coefficient became smaller, and the liver coefficient of the high-dose metadoxine group, the medium-dose sample solution group and the high-dose sample solution group were all lower than that of the model group, and there were significant differences compared with the model group (P < 0.01); at the same time, it can be seen that compared with the metadoxine groups, the sample solution groups had a better recovery effect on the increase of liver coefficient caused by alcohol, and accelerated the protection of the liver.

[0118] 2.4.6.2 Effects on the activity of alcohol dehydrogenase in mouse liver

[0119] The results of the effects of each group on the activity of alcohol dehydrogenase in the liver of mice are as follows Figure 5 As shown in Table 2. Note: s: compared with the blank control group, P < 0.05; ss: compared with the blank control group, P < 0.01; *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0120] See also Figure 5 As shown in Table 2, compared with the blank control group, the alcohol dehydrogenase activity in the liver of the model group mice was significantly lower than that of the blank control group mice (P < 0.01), while the alcohol dehydrogenase activity in the liver of the low-dose metadoxine group, the medium-dose metadoxine group and the low-dose sample solution group mice was not significantly different from that of the model group (P > 0.05). However, with the increase of the dose of the oral intervention substance, the alcohol dehydrogenase activity increased, and there was a significant difference in the high-dose metadoxine group (P < 0.05). The alcohol dehydrogenase activity in the liver of the mice in the medium-dose sample solution group and the high-dose sample solution group was significantly different from that in the model group, and the high-dose sample solution group had a very significant difference (P < 0.01). At the same time, it can be seen that after drinking, compared with the metadoxine groups, the sample solution groups can quickly increase the alcohol dehydrogenase activity and accelerate the sobering up.

[0121] 2.4.6.3 Effects on the activity of acetaldehyde dehydrogenase in mouse liver

[0122] The results of the effects of each group on the activity of acetaldehyde dehydrogenase in mouse liver are as follows Figure 6 As shown in Table 2. Among them: s: compared with the blank control group, P < 0.05; ss: compared with the blank control group, P < 0.01; *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0123] Depend on Figure 6 As shown in Table 2, compared with the blank control group, the acetaldehyde dehydrogenase activity in the liver of the model group mice was significantly lower than that of the blank control group mice (P < 0.01), while the acetaldehyde dehydrogenase activity in the liver of the mice in the low-dose group of metadoxine and the low-dose group of sample solution was not significantly different from that in the model group (P > 0.05). However, with the increase of the dose of the oral intervention, the acetaldehyde dehydrogenase activity increased. The acetaldehyde dehydrogenase activity in the liver of the mice in the medium-dose group of metadoxine was not significantly different from that in the model group (P > 0.05), and the high-dose group had a very significant difference (P < 0.01). The acetaldehyde dehydrogenase activity in the liver of the mice in the medium-dose group of sample solution and the high-dose group of sample solution was significantly different from that in the model group, and the high-dose group had a very significant difference (P < 0.01). At the same time, it can be seen that after drinking, compared with the metadoxine groups, the sample solution groups can quickly increase the acetaldehyde dehydrogenase activity and accelerate the detoxification.

[0124] 2.4.6.4 Effects on malondialdehyde (MDA) in mouse liver

[0125] The results of the effects of each group on malondialdehyde (MDA) in mouse liver are as follows Figure 7 As shown in Table 2. Among them: s: compared with the blank control group, P < 0.05; ss: compared with the blank control group, P < 0.01; *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0126] Depend on Figure 7 As shown in Table 2, compared with the blank control group, the content of malondialdehyde (MDA) in the liver of mice in the model group was significantly lower than that in the blank control group (P < 0.05), while the content of malondialdehyde (MDA) in the liver of mice in the low-dose metadoxine group and the low-dose sample solution group was not significantly different from that in the model group (P > 0.05). However, with the increase of the dose of the oral intervention substance, the content of malondialdehyde (MDA) in the liver of mice in the medium-dose metadoxine group and the high-dose metadoxine group was significantly different from that in the model group, and the high-dose group had a very significant difference (P < 0.01). The content of malondialdehyde (MDA) in the liver of mice in the medium-dose sample solution group and the high-dose sample solution group was very significantly different from that in the model group (P < 0.01). At the same time, it can be seen that after drinking, the sample solution groups had a significantly better effect on reducing the content of malondialdehyde (MDA) than the metadoxine groups.

[0127] 2.4.6.5 Effects on reduced glutathione (GSH) in mouse liver

[0128] The results of the effects of each group on reduced glutathione (GSH) in mouse liver are as follows Figure 8 As shown in Table 2. Among them: s: compared with the blank control group, P < 0.05; ss: compared with the blank control group, P < 0.01; *: compared with the model group, P < 0.05; **: compared with the model group, P < 0.01.

[0129] Depend on Figure 8As shown in Table 2, compared with the blank control group, the reduced glutathione (GSH) in the liver of the model group mice was significantly lower than that of the blank control group mice (P < 0.01); compared with the model group, there was no significant difference in the reduced glutathione (GSH) in the liver of the mice in the low-dose metadoxine group, the medium-dose metadoxine group, the high-dose metadoxine group, the low-dose sample solution group, and the medium-dose sample solution group (P > 0.05). However, with the increase in the dose of the oral intervention substance, the reduced glutathione (GSH) in the liver of the mice in the high-dose sample solution group was significantly different from that in the model group (P < 0.01). At the same time, it can be seen that after drinking, the sample solution groups were significantly better than the metadoxine groups in increasing the content of reduced glutathione (GSH).

[0130] Table 2 Effects on liver coefficient, ADH, ALDH, GSH, and MDA in mice (n=10)

[0131]

[0132] Comparing the above data, it can be seen that, relative to the model group, the use of metadoxine and the sample solution has an inhibitory effect on the increase in liver coefficient caused by alcohol, making the liver coefficient close to normal; both can improve the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase, and can reduce the content of malondialdehyde (MDA) and increase the content of reduced glutathione (GSH); indicating that metadoxine and the sample solution have a hangover effect; but the sample solution has a significantly better effect on the liver coefficient, alcohol dehydrogenase activity, acetaldehyde dehydrogenase activity, malondialdehyde (MDA) content and reduced glutathione (GSH) content than metadoxine. It shows that the kudzu root and Hovenia dulcis plant beverage provided by the present invention can better promote the decomposition and metabolism of ethanol and acetaldehyde in the liver, has the effect of rapid alcohol detoxification, has a protective effect on mouse liver tissue, and avoids the damage caused by alcohol to the body.

[0133] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the present invention, although the present invention has been described in detail with reference to the preferred embodiments. It should be understood by those skilled in the art that the technical solutions of the present invention can still be modified or replaced by equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A Pueraria root and Hovenia dulcis plant beverage, characterized in that: The invention is composed of raw materials in the following mass ratios: kudzu root, hovenia dulcis fruit, tuckahoe, tangerine peel, turmeric, reed root and mulberry in the ratio of (1-10): (1-10): (0.5-5): (0.5-6): (0.01-1): (0.1-5): (0.1-5).

2. A Pueraria root and Hovenia dulcis plant beverage, characterized in that: The medicine is composed of the following raw materials in the following mass ratios: Pueraria root, Hovenia dulcis fruit, Poria cocos, dried orange peel, turmeric, reed root and mulberry in a ratio of 3:3:2:2:0.5:1.5:1.

5.

3. The method for preparing the Pueraria lobata and Hovenia dulcis plant beverage according to claim 1, characterized in that: The following steps are involved: S1. Prepare the raw materials according to the above mass ratio: Pueraria root, Hovenia dulcis, Poria cocos, dried orange peel, turmeric, Phragmites australis and mulberry; S2. For each of the above raw materials, a decoction method is used to obtain an extract; S3, concentrating the extract under normal pressure to obtain an extract solution; S4. Add sucralose and xylitol to the extract, mix evenly, filter, and then obtain the filtrate through filling, sterilization, and cooling to obtain the Pueraria lobata and Hovenia dulcis plant beverage; add 2g-10g of xylitol and 1mg-3mg of sucralose to every 100ml of the extract.

4. The method for preparing the Pueraria lobata and Hovenia dulcis plant beverage according to claim 3, characterized in that: The specific process of step S2 is: Mix all the raw materials, then soak them in water for 30 minutes, then add water again, perform the first extraction at 100°C, and collect the first filtrate and the first residue; Continue to add water to the primary filter residue, perform a second extraction at 100°C, and collect the secondary filtrate and secondary filter residue; Continue to add water to the secondary filter residue, perform a third extraction at 100°C, collect the three filtrates, and discard the three filter residues; combine the obtained first filtrate, second filtrate and third filtrate to obtain an extract.

5. The method for preparing the Pueraria lobata and Hovenia dulcis plant beverage according to claim 4, characterized in that: In step S3, during each extraction, the mass ratio of solid material to water is 1:12; the solid material is raw material or filter residue; and the extraction time is 1.5 hours each time.

6. The method for preparing the Pueraria lobata and Hovenia dulcis plant beverage according to claim 3, characterized in that: In step S3, atmospheric pressure concentration is performed at 100° C. and concentrated until 100 ml of the extract contains 27 g of the raw material.

7. Use of the Pueraria lobata and Hovenia dulcis plant beverage according to claim 1 in sobering up.

8. Use of the Pueraria lobata and Hovenia dulcis plant beverage as claimed in claim 1 in sobering up by prolonging the latent period of drunkenness and shortening the sobering time.

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