Polypeptide synthesis equipment

Abstract

The embodiment of the invention provides polypeptide synthesis equipment. The polypeptide synthesis equipment comprises a reaction container assembly, the reaction container assembly comprises a reaction cup and a base installed below the reaction cup, the reaction cup is provided with a bottom opening, and the base is provided with a channel communicated with the bottom opening; and the bubbling assembly is used for bubbling gas into the reaction cup from the bottom opening through the channel. In practical application of the polypeptide synthesis equipment, a piperidine solution and resin can be added into the reaction cup. The piperidine solution and the resin can react in the reaction cup. The bubbling assembly may then blow gas through the channel and then into the reaction cup from the bottom opening. As the bottom opening is located at the bottom of the reaction cup, gas blown by the bubbling assembly can directly stir a piperidine solution at the bottom of the reaction cup. As the gas rises in the reaction cup, the piperidine solution in the reaction cup can be completely stirred by the gas. The piperidine solution can be in full contact with the resin, and the reaction speed of the two can be greatly increased.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide synthesis, and in particular to a polypeptide synthesis device. Background Art

[0002] As a key area of ​​innovative research and development in biomedicine, peptide synthesis technology has broad development prospects. Peptide drugs have become a hot spot in global new drug research and development due to their high biological activity, high specificity, low side effects, and low accumulation in the body. Currently, more than 80 peptide drugs have been approved for marketing worldwide, with a market size of US$62.8 billion in 2020 and expected to grow to US$96 billion in 2025, showing a strong growth momentum.

[0003] Traditional peptide synthesis equipment includes a reaction cup. A resin is placed in the reaction cup. The resin can be used as a solid phase carrier, which is usually pre-activated and has a protecting group such as Fmoc (fluorenylmethoxycarbonyl). A 20% piperidine solution can be added to the reaction cup, and the piperidine solution can deprotect the resin and remove the Fmoc protecting group at the N-terminus. Then, a gas such as nitrogen can be added to the reaction cup so that bubbling (condensation reaction) can be performed. Then, subsequent steps can be performed to eventually connect all amino acids to the resin in a predetermined order to form a complete polypeptide chain.

[0004] However, the gas added into the reaction cup is difficult to reach the bottom of the reaction cup, so that the piperidine solution at the bottom of the reaction cup cannot be stirred, which leads to the failure of the piperidine solution and the resin to fully react, resulting in poor reaction effect. Summary of the invention

[0005] In order to at least partially solve the problems existing in the prior art, an embodiment of the present invention provides a polypeptide synthesis device. The polypeptide synthesis device includes: a reaction vessel assembly, the reaction vessel assembly includes a reaction cup and a base installed below the reaction cup, the reaction cup has a bottom opening, and the base has a channel connected to the bottom opening; and a bubbling assembly, the bubbling assembly is used to bubbling gas from the bottom opening into the reaction cup through the channel.

[0006] Exemplarily, the reaction container assembly has multiple groups, each of the multiple groups includes at least one reaction cup and a base corresponding one-to-one to the reaction cup, and the bubbling assembly has multiple bubbling assemblies corresponding one-to-one to the multiple groups, and each bubbling assembly is used to bubble gas into the reaction cup from the bottom opening through each channel in its corresponding group.

[0007] Exemplarily, at least two of the multiple groups are staggered in the vertical direction.

[0008] Exemplarily, the bubbling assembly includes a gas source triplet, a shut-off valve and a speed regulating valve. Along the flow direction of the gas, the gas source triplet, the shut-off valve, the speed regulating valve and the channel are connected in sequence.

[0009] Exemplarily, the speed regulating valve includes a manual speed regulating valve, which is located outside the reaction vessel assembly.

[0010] Exemplarily, the reaction container assembly includes multiple reaction cups, and the bubbling assembly also includes a diverter, on which a main channel and multiple branch channels are provided, the shut-off valves are connected to both ends of the main channel, one ends of the multiple branch channels are respectively connected to the middle between the two ends of the main channel, and the speed regulating valves are connected one by one between the other ends of the multiple branch channels and the channels.

[0011] Exemplarily, the bubbling assembly further includes a three-way connector and a pair of shunt pipes, wherein the three-way connector is respectively connected to the shut-off valve and one end of the pair of shunt pipes, and the other ends of the pair of shunt pipes are connected to the two ends of the main channel one by one.

[0012] Exemplarily, the base includes a supporting member and a feed tube, the reaction cup is supported on the supporting member, the feed tube extends downward from the supporting member, the channel is arranged in the feed tube, the side of the feed tube is provided with a liquid inlet and an air inlet connected to the channel, and the bubbling assembly is connected to the air inlet.

[0013] Exemplarily, along a lateral direction perpendicular to the extension direction of the feed pipe, the liquid inlet and the gas inlet are arranged opposite to each other.

[0014] Exemplarily, along the extension direction of the feed pipe, the liquid inlet and the gas inlet are completely staggered.

[0015] Exemplarily, the supporting member includes a cup holder and a sealing ring, the reaction cup includes a large-size segment at the upper end, a small-size segment at the lower end, and a transition segment connected between the upper end and the lower end, the transition segment is supported on the cup holder, the small-size segment is inserted into the cup holder, the sealing ring surrounds the small-size segment and is clamped between the cup holder and the small-size segment, and the bottom opening is set at the bottom of the small-size segment.

[0016] Exemplarily, one-way valves are respectively disposed in the liquid inlet and the air inlet.

[0017] Exemplarily, the reaction cup includes a large-sized section at the upper end, a small-sized section at the lower end, and a transition section connected between the upper end and the lower end, and a filter net supported on the transition section is arranged in the reaction cup.

[0018] In practical applications of the peptide synthesis device, a piperidine solution and a resin can be added to the reaction cup. The piperidine solution and the resin can react in the reaction cup. Then, the bubbling component can pass the gas through the channel and then blow it into the reaction cup from the bottom opening. Since the bottom opening is located at the bottom of the reaction cup, the gas blown in by the bubbling component can directly stir the piperidine solution at the bottom of the reaction cup. Moreover, as the gas rises in the reaction cup, the piperidine solution in the reaction cup can be completely stirred by the gas. In this way, the piperidine solution and the resin can be fully contacted, and the reaction rate of the two can be greatly improved. Therefore, the peptide synthesis device can increase the reaction rate, thereby improving the production efficiency of the peptide. The peptide synthesis device can increase the throughput of peptide production.

[0019] A series of simplified concepts are introduced in the summary of the invention, which will be further described in detail in the detailed description. The summary of the invention does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0020] The advantages and features of the present invention are described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The following drawings of the present invention are used as part of the present invention for understanding the present invention. The drawings show the embodiments of the present invention and their descriptions, and are used to explain the principles of the present invention. In the drawings,

[0022] Figure 1 A perspective view of a polypeptide synthesis device according to an exemplary embodiment of the present invention;

[0023] Figure 2 for Figure 1 A perspective view of a partial structure of a peptide synthesis device shown in FIG.

[0024] Figure 3 for Figure 2 A perspective view of the reaction vessel assembly and the bubbling assembly shown in FIG.

[0025] Figure 4 for Figure 3 A front view of the reaction vessel assembly shown in;

[0026] Figure 5 for Figure 4 A cross-sectional view of a reaction vessel assembly shown in FIG.

[0027] Figure 6 for Figure 4 A front view of the reaction cup shown in FIG.

[0028] Figure 7 for Figure 6 A cross-sectional view of a reaction cup is shown in FIG.

[0029] The above drawings include the following reference numerals:

[0030] 100, reaction vessel assembly; 300, reaction cup; 301, bottom opening; 302, top opening; 310, large size section; 320, small size section; 330, transition section; 400, supporting member; 410, cup holder; 420, sealing ring; 500, feeding pipe; 510, liquid inlet; 520, air inlet; 600, filter; 700, bubbling assembly; 710, speed regulating valve; 720, diverter; 721, main channel; 722, branch channel; 730, three-way connector; 740, diverter pipeline; 750, branch pipeline; 800, base; 801, channel. DETAILED DESCRIPTION

[0031] In the following description, a large amount of details are provided so that the present invention can be thoroughly understood. However, it will be appreciated by those skilled in the art that the following description only exemplarily illustrates a preferred embodiment of the present invention, and the present invention can be implemented without one or more such details. In addition, in order to avoid confusion with the present invention, some technical features well known in the art are not described in detail.

[0032] The embodiment of the present invention provides a peptide synthesis device. The peptide synthesis device can be used to perform a peptide synthesis reaction, and thus can be used to synthesize peptides. The peptide synthesis device of the embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0033] like Figure 1-3 As shown, the polypeptide synthesis device may include a reaction vessel component 100 and a bubbling component 700 .

[0034] The reaction vessel assembly 100 may include a reaction cup 300 and a base 800. The reaction cup 300 may have a bottom opening 301. That is, the bottom opening 301 may be provided at the bottom of the reaction cup 300. Materials such as liquid (e.g., piperidine solution) and / or gas (e.g., nitrogen) may enter the reaction cup 300 through the bottom opening 301. The reaction cup 300 may be used for a polypeptide synthesis reaction. Exemplarily, the reaction cup 300 may be made of glass or other transparent materials. With such a configuration, relevant personnel may conveniently observe the reaction conditions inside the reaction cup 300 from the outside of the reaction cup 300. Exemplarily, a top opening 302 may be provided at the top of the reaction cup 300. Materials such as resin may enter the reaction cup 300 through the top opening 302. Of course, materials such as resin may also enter the reaction cup 300 through the bottom opening 301 in advance, or may be placed in the reaction cup 300 in advance during the manufacturing process of the reaction cup 300. The top opening 302 may also be used as a spare opening to provide users with more options. The base 800 can be installed below the reaction cup 300. The reaction cup 300 can be simply seated on the base 800, or can be connected to the base 800 by plugging, snapping or any other suitable method. The base 800 can have a channel 801. The channel 801 can be connected to the bottom opening 301 of the reaction cup 300.

[0035] The bubbling assembly 700 can be used to be connected to a gas storage device. In this way, the gas storage device can provide gas, and the bubbling assembly 700 can pass the gas through the channel 801 and then bubbling the gas into the reaction cup 300 from the bottom opening 301.

[0036] In practical applications of the peptide synthesis device, a piperidine solution and a resin can be added to the reaction cup 300. The piperidine solution and the resin can react in the reaction cup 300. Then, the bubbling component 700 can pass the gas through the channel 801 and then bubbling it into the reaction cup 300 from the bottom opening 301. Since the bottom opening 301 is located at the bottom of the reaction cup 300, the gas blown in by the bubbling component 700 can directly stir the piperidine solution at the bottom of the reaction cup 300. Moreover, as the gas rises in the reaction cup 300, the piperidine solution in the reaction cup 300 can be completely stirred by the gas. In this way, the piperidine solution and the resin can be fully contacted, and the reaction speed of the two can be greatly improved. Therefore, the peptide synthesis device can increase the reaction speed, thereby improving the production efficiency of the polypeptide. The peptide synthesis device can increase the throughput of polypeptide production.

[0037] For example, Figure 1-3As shown, the reaction vessel assembly 100 may have multiple groups, including but not limited to the six groups shown in the figure, for example, two groups, three groups or other. Each group of reaction vessel assemblies 100 in the multiple groups may include at least one reaction cup 300, for example, one, two or more. In the embodiment where each group of reaction vessel assemblies 100 includes multiple reaction cups 300, the multiple reaction cups 300 may be arranged in a straight line direction. In the embodiment shown in the figure, each group of reaction vessel assemblies 100 may include ten reaction cups 300. Different groups of reaction vessel assemblies 100 may have the same or different numbers of reaction cups 300. Regardless of the same group or different groups, the structures of different reaction cups 300 may be the same or different, and the same or different reactions may be performed in different reaction cups 300. The reaction cup 300 and the base 800 may be arranged one-to-one. Each base 800 may be installed below its corresponding reaction cup 300. In this way, the channel 801 of each base 800 may be connected to the bottom opening 301 of the reaction cup 300 in a one-to-one correspondence. Regardless of being in the same group or different groups, the structures of different bases 800 may be the same or different. The bubbling assembly 700 may have a plurality of bubbling assemblies corresponding one to one to multiple groups of reaction vessel assemblies 100. That is, each group of reaction vessel assemblies 100 may correspond to one bubbling assembly 700. Each bubbling assembly 700 may be used to blow gas from the bottom opening 301 into the reaction cup 300 through each channel 801 in its corresponding group. Specifically, for each group of reaction vessel assemblies 100, the bubbling assembly 700 may blow gas into all channels 801, and then may blow gas into the corresponding reaction cup 300 through all bottom openings 301. With such a configuration, the polypeptide synthesis device may perform multiple groups of reactions simultaneously, thereby improving the throughput of synthesizing polypeptides.

[0038] For example, Figure 1-3 As shown, at least two groups of reaction vessel assemblies 100 in the plurality of groups can be staggered in the vertical direction. The reaction vessel assemblies 100 staggered in the vertical direction can facilitate the routing of pipelines such as air pipes and liquid pipes. In addition, it is convenient for relevant personnel to observe reaction cups 300 at different heights. It should be noted that, as long as there are two groups of reaction vessel assemblies 100 in the plurality of groups staggered in the vertical direction. In other words, when the reaction vessel assembly 100 has more groups, the other groups of reaction vessel assemblies 100 can be arranged in any suitable position, for example, they can be staggered in the horizontal direction with one of the two groups of reaction vessel assemblies 100 staggered in the vertical direction.

[0039] For example, Figure 1-3As shown, the bubbling assembly 700 may include a gas source triplet (not shown), a stop valve (not shown) and a speed regulating valve 710. Along the flow direction of the gas, the gas source triplet, the stop valve, the speed regulating valve 710 and the channel 801 may be connected in sequence. The gas source triplet can be used to connect to a gas storage device. Therefore, the bubbling assembly 700 may also include a gas storage device. The gas may pass through the gas source triplet, the stop valve, the speed regulating valve 710, the channel 801 and the bottom opening 301 in sequence, so as to enter the reaction cup 300. The gas source triplet can purify, filter and reduce the pressure of the gas passing through to the expected pressure. The stop valve can be used to control the on and off of the gas. The speed regulating valve 710 can be used to adjust the flow of the gas, so that the reaction speed in the reaction cup 300 can be controlled by controlling the air intake in the reaction cup 300. The speed regulating valve 710 includes but is not limited to an electromagnetic speed regulating valve or a manual speed regulating valve.

[0040] For example, Figure 1-3 As shown, the speed regulating valve 710 may include a manual speed regulating valve, which has a relatively simple structure and low cost. The manual speed regulating valve may be located on the outside of the reaction vessel assembly 100 to facilitate relevant personnel to adjust the manual speed regulating valve. The directional term "outside" used below generally refers to the side close to the relevant personnel. In other words, relevant personnel can usually perform relevant work on the outside of the reaction vessel assembly 100. Therefore, the directional term "inside" used below generally refers to the side away from the relevant personnel. It should be noted that, Figure 2 As shown, the two groups of reaction vessel assemblies 100 in the figure can be symmetrically arranged. Therefore, the upper left side of the upper reaction vessel assembly 100 can be its outer side, and the lower right side of the lower reaction vessel assembly 100 can be its outer side.

[0041] For example, Figure 2-3As shown, the reaction container assembly 100 may include a plurality of reaction cups 300. The bubbling assembly 700 may also include a flow divider 720. The flow divider 720 may be provided with a main channel 721 and a plurality of branch channels 722. The branch channels 722 may be provided in a one-to-one correspondence with the bottom opening 301. The main channel 721 may pass through the flow divider 720. One end of the plurality of branch channels 722 may be connected to the middle between the two ends of the main channel 721. One end of the stop valve may be connected to the gas source triplet. The other end of the stop valve may be connected to the two ends of the main channel 721. The speed regulating valve 710 may be provided in a one-to-one correspondence with the branch channels 722. The speed regulating valve 710 may be connected in a one-to-one correspondence between the other end of the plurality of branch channels 722 and the channel 801. In this way, after the gas passes through the stop valve, it may enter the flow divider 720 from the two ends of the main channel 721, and then may flow out through the plurality of branch channels 722, respectively. The gas passing through the branch channel 722 can pass through its corresponding speed regulating valve 710 and channel 801 respectively, and then enter the reaction cup 300 through the bottom opening 301. In this way, each speed regulating valve 710 can individually adjust the flow rate on the gas path where it is located, so that the intake volume of different reaction cups 300 can be the same or different to meet different needs. In addition, since the gas enters through both ends of the main channel 721 and then flows out through multiple branch channels 722 respectively, the gas can flow to each branch channel 722 relatively evenly, thereby avoiding the problem of too little gas passing through the downstream branch channel 722.

[0042] For example, Figure 2-3 As shown, the bubbler assembly 700 may further include a three-way joint 730 and a pair of shunt pipes 740. One end of the three-way joint 730 may be connected to the stop valve. The other two ends of the three-way joint 730 may be connected to the two ends of the main channel 721 through a pair of shunt pipes 740 in a one-to-one correspondence. The bubbler assembly 700 may further include a branch pipe 750. The branch pipe 750 may be connected between the branch channel 722 and the channel 801 in a one-to-one correspondence. The speed regulating valve 710 may be arranged on its corresponding branch pipe 750.

[0043] For example, Figure 4-5As shown, the base 800 may include a support member 400 and a feed pipe 500. The reaction cup 300 may be supported on the support member 400. The support member 400 may serve as a base for the reaction vessel assembly 100. The support member 400 may be used to be fixed to a component outside the reaction vessel assembly 100. The feed pipe 500 may extend downward from the support member 400. The feed pipe 500 may be connected to the bottom of the support member 400 by any suitable means such as welding, bonding or integral molding. A channel 801 may be arranged in the feed pipe 500. A liquid inlet 510 and an air inlet 520 may be provided on the side of the feed pipe 500. The liquid inlet 510 and the air inlet 520 may both be connected to the channel 801. In some embodiments, the channel 801 may extend in a vertical direction. The top of the channel 801 may be aligned with and connected to the bottom opening 301. The lower end of the channel 801 may be connected to the liquid inlet 510 and the air inlet 520. In this way, the channel 801 can be connected between the bottom opening 301 and the liquid inlet 510 and the gas inlet 520. The liquid can enter the channel 801 through the liquid inlet 510, and then enter the reaction cup 300 through the bottom opening 301. The liquid inlet 510 can be used to be connected to the liquid storage device. The bubbling assembly 700 can be connected to the gas inlet 520. The gas blown by the bubbling assembly 700 can enter the channel 801 through the gas inlet 520, and then enter the reaction cup 300 through the bottom opening 301. By providing the liquid inlet 510 and the gas inlet 520, the feeding tube 500 can have a basis for being connected to the liquid storage device and the gas storage device respectively, so that there is no need to switch the connection between the liquid storage device and the gas storage device, and the user's experience is better. And, if necessary, the liquid and the gas can enter the reaction cup 300 through the bottom opening 301 at the same time.

[0044] In actual application of the peptide synthesis equipment, like gas, the liquid will rise from the bottom of the reaction cup 300 during the process of entering, so it will not splash onto the wall of the reaction cup 300. In this way, when the reaction cup 300 is used for subsequent processes, the wall of the reaction cup 300 will not be contaminated by the liquid flowing down. In addition, there is no need to set a pipeline for introducing liquid and gas into the reaction cup 300, and the space in the reaction cup 300 can be fully used for reaction, and the utilization rate of the space in the reaction cup 300 is high.

[0045] For example, Figure 4-5As shown, along the lateral direction of the feed tube 500, the liquid inlet 510 and the air inlet 520 can be arranged relatively. The lateral direction can be perpendicular to the extension direction of the feed tube 500. Generally speaking, the extension direction of the feed tube 500 can be a vertical direction. Therefore, the lateral direction can be a horizontal direction. In this way, the distance between the liquid inlet 510 and the air inlet 520 is far, so that there can be enough space for connection with the liquid storage device and the bubbling assembly 700. Exemplarily, the liquid inlet 510 can be arranged on the inner side of the feed tube 500. The air inlet 520 can be arranged on the outer side of the feed tube 500.

[0046] For example, Figure 4-5 As shown, along the extension direction of the feed pipe 500, the liquid inlet 510 and the gas inlet 520 can be completely staggered. In this way, the liquid inlet 510 and the gas inlet 520 will not be directly opposite to each other. In this way, when the liquid enters the feed pipe 500 through the liquid inlet 510, the liquid will not directly rush to the gas inlet 520, thereby preventing the liquid from flowing out through the gas inlet 520. Similarly, when the gas enters the feed pipe 500 through the gas inlet 520, the gas will not directly rush to the liquid inlet 510, thereby preventing the liquid from flowing out through the liquid inlet 510.

[0047] For example, Figure 4-5As shown, the support member 400 may include a cup holder 410 and a sealing ring 420. The cup holder 410 may be annular. The cup holder 410 may be used to fix to a component outside the reaction vessel assembly 100. The feed pipe 500 may extend downward from the cup holder 410. The feed pipe 500 may be connected to the bottom of the cup holder 410 by any suitable means such as welding, bonding or integral molding. The reaction cup 300 may include a large-size section 310, a small-size section 320 and a transition section 330. The large-size section 310 may be located at the upper end of the reaction cup 300. The small-size section 320 may be located at the lower end of the reaction cup 300. The transition section 330 may be connected between the upper end and the lower end of the reaction cup 300. That is, in the vertical direction, the large-size section 310, the transition section 330 and the small-size section 320 may be connected in sequence. In some embodiments, the large-size section 310 and the transition section 330 may be smoothly transitioned. The small-size segment 320 and the transition segment 330 may have a smooth transition. The bottom opening 301 may be provided at the bottom of the small-size segment 320. The top opening 302 may be provided at the top of the large-size segment 310. The small-size segment 320 may be inserted into the cup holder 410. The transition segment 330 may be supported on the cup holder 410. The sealing ring 420 may surround the small-size segment 320. In addition, the sealing ring 420 may be clamped between the cup holder 410 and the small-size segment 320. In practical applications, the sealing ring 420 may be pre-placed in the cup holder 410. When the reaction cup 300 needs to be installed, the small-size segment 320 may be first inserted into the sealing ring 420 in the cup holder 410, so that the sealing ring 420 may be clamped between the cup holder 410 and the small-size segment 320. Following the insertion, when the transition segment 330 is supported on the cup holder 410, the reaction cup 300 is installed in place. With such a configuration, the reaction cup 300 can be easily installed and disassembled. In addition, the sealing ring 420 can exert a greater friction force on the small-size segment 320, thereby preventing the reaction cup 300 from being easily displaced and ensuring that the reaction cup 300 is in the expected position. In addition, the sealing ring 420 can also seal the gap between the small-size segment 320 and the cup holder 410 to prevent the liquid and gas passing through the feeding tube 500 from leaking through the gap due to excessive pressure. The sealing ring 420 can adopt various types of sealing rings known in the art or that may appear in the future, including but not limited to pan seals. The material of the sealing ring 420 can be arbitrary, including but not limited to polytetrafluoroethylene (PTFE).

[0048] For example, Figure 6-7As shown, a filter screen 600 may be provided in the reaction cup 300. The filter screen 600 may be supported on the transition section 330 by any suitable means such as welding or bonding. The filter screen 600 may be used to support materials such as resin. The liquid and gas passing through the feed pipe 500 may pass through the filter screen 600, so as to react with the materials such as resin supported on the filter screen 600. Exemplarily, the filter screen 600 may adopt a sand core of about 40 meshes, so as to be used to support resin particles with a mesh size of about 100-200 meshes.

[0049] For example, a one-way valve may be provided in the liquid inlet 510 and the gas inlet 520. The one-way valves provided in the liquid inlet 510 and the gas inlet 520 may be the same or different. In this way, liquid will not flow back from the liquid inlet 510, and gas will not flow back from the gas inlet 520.

[0050] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by directional words such as "front", "back", "up", "down", "left", "right", "lateral", "vertical", "vertical", "horizontal", "top", "bottom", etc. is usually based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention; the directional words "inside" and "outside" refer to the inside and outside relative to the contour of each component itself.

[0051] For ease of description, regional relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the regional positional relationship between one or more components or features shown in the figure and other components or features. It should be understood that regional relative terms include not only the orientation of the components as described in the figure, but also different orientations in use or operation. For example, if the components in the accompanying drawings are inverted as a whole, the components "above other components or features" or "above other components or features" will include the situation where the components are "below other components or structures" or "below other components or structures". Therefore, the exemplary term "above" may include both "above" and "below". In addition, these components or features may also be positioned at other different angles (e.g., rotated 90 degrees or other angles), and this article is intended to include all of these situations.

[0052] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, parts, components and / or combinations thereof.

[0053] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0054] The present invention has been described by the above embodiments, but it should be understood that the above embodiments are only for the purpose of example and description, and are not intended to limit the present invention to the scope of the described embodiments. In addition, it can be understood by those skilled in the art that the present invention is not limited to the above embodiments, and more variations and modifications can be made according to the teachings of the present invention, and these variations and modifications all fall within the scope of the protection claimed by the present invention. The protection scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A polypeptide synthesis device, characterized in that: include: A reaction container assembly, the reaction container assembly comprising a reaction cup and a base installed below the reaction cup, the reaction cup having a bottom opening, and the base having a channel communicating with the bottom opening; as well as A bubbling assembly is used to bubble gas into the reaction cup from the bottom opening through the channel.

2. The peptide synthesis device according to claim 1, characterized in that: The reaction container assembly has multiple groups, each of the multiple groups includes at least one reaction cup and a base corresponding to the reaction cup one by one, and the bubbling assembly has multiple bubbling assemblies corresponding to the multiple groups one by one, and each bubbling assembly is used to bubble gas into the reaction cup from the bottom opening through each channel in its corresponding group.

3. The polypeptide synthesis device according to claim 2, characterized in that: At least two of the multiple groups are staggered in the vertical direction.

4. The peptide synthesis device according to claim 1, characterized in that: The bubbling assembly comprises a gas source triplet, a stop valve and a speed regulating valve. Along the flow direction of the gas, the gas source triplet, the stop valve, the speed regulating valve and the channel are connected in sequence.

5. The polypeptide synthesis device according to claim 4, characterized in that: The speed regulating valve comprises a manual speed regulating valve, and the manual speed regulating valve is located outside the reaction container assembly.

6. The polypeptide synthesis device according to claim 4, characterized in that: The reaction container assembly includes a plurality of reaction cups, and the bubbling assembly also includes a diverter, on which a main channel and a plurality of branch channels are provided, the shut-off valve is connected to both ends of the main channel, one ends of the plurality of branch channels are respectively connected to the middle portion between the two ends of the main channel, and the speed regulating valve is connected one-to-one between the other ends of the plurality of branch channels and the channel.

7. The peptide synthesis device according to claim 6, characterized in that: The bubbling assembly also includes a three-way joint and a pair of shunt pipes, the three-way joint is respectively connected to the stop valve and one end of the pair of shunt pipes, and the other ends of the pair of shunt pipes are connected to the two ends of the main channel one by one.

8. The peptide synthesis device according to claim 1, characterized in that: The base includes a supporting member and a feed tube, the reaction cup is supported on the supporting member, the feed tube extends downward from the supporting member, the channel is arranged in the feed tube, a liquid inlet and an air inlet connected to the channel are arranged on the side of the feed tube, and the bubbling assembly is connected to the air inlet.

9. The polypeptide synthesis device according to claim 8, characterized in that: Along a lateral direction perpendicular to the extending direction of the feed pipe, the liquid inlet and the air inlet are arranged opposite to each other.

10. The peptide synthesis device according to claim 8, characterized in that: Along the extending direction of the feed pipe, the liquid inlet and the gas inlet are completely staggered.

11. The peptide synthesis device according to claim 8, characterized in that: The supporting member includes a cup holder and a sealing ring, the reaction cup includes a large-size segment at the upper end, a small-size segment at the lower end, and a transition segment connected between the upper end and the lower end, the transition segment is supported on the cup holder, the small-size segment is inserted into the cup holder, the sealing ring surrounds the small-size segment and is clamped between the cup holder and the small-size segment, and the bottom opening is arranged at the bottom of the small-size segment.

12. The peptide synthesis device according to claim 8, characterized in that: One-way valves are respectively arranged in the liquid inlet and the air inlet.

13. The peptide synthesis device according to claim 8, characterized in that: The reaction cup comprises a large-sized section at the upper end, a small-sized section at the lower end, and a transition section connected between the upper end and the lower end. A filter net supported on the transition section is arranged in the reaction cup.

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