A method for processing glycine fermentation liquor and application thereof in fertilizer preparation

Abstract

The application discloses a glycine fermentation liquor treatment method and application in fertilizer preparation, and relates to the technical field of fertilizer preparation, and comprises the following steps: step one, introducing the glycine fermentation liquor into a treatment container with good corrosion resistance and heat preservation performance; firstly, the fermentation liquor is subjected to preliminary detection to determine initial components, concentration and pH value parameters; the application has the advantages that: by introducing specific biological enzyme auxiliary separation technology extracted from multiple microbial fermentation, in combination with a novel adsorption material with high selective adsorption capacity, efficient separation and resource recovery of various components in the glycine fermentation liquor are realized; the biological enzyme can selectively degrade complex organic matters in the fermentation liquor, so that the complex organic matters are more easily separated; the novel adsorption material can efficiently adsorb glycine in a targeted manner, the use of chemical reagents is reduced, and environmental pollution is greatly reduced; the method not only effectively improves the recovery rate of glycine, but also significantly reduces the emission of pollutants.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of fertilizer preparation, in particular to a processing method of glycine fermentation liquor and application thereof in fertilizer preparation. BACKGROUND

[0002] Glycine, as an important amino acid, plays a key role in many fields such as medicine, food, chemical industry, etc. It is not only a synthetic intermediate of many drugs, but also widely used in food additives, feed additives and cosmetics industries. Due to its wide application and market demand, the production of glycine has attracted much attention. Fermentation is one of the main methods for producing glycine at present. The processing of glycine fermentation liquor is a crucial step in the fermentation production process of glycine, which directly affects the quality, yield and cost of the final product. The traditional processing method of glycine fermentation liquor has many limitations and shortcomings.

[0003] The existing processing method of glycine fermentation liquor has certain defects. First, the separation efficiency of glycine fermentation liquor components is low in the prior art, resource recovery is difficult, and many chemical reagents are used, which causes serious environmental pollution. Second, the foam in the prior art may affect the processing process, resulting in low utilization rate of fermentation liquor volume, uneven stirring, unstable product quality. Third, in the processing of glycine fermentation liquor, the energy consumption is high, the time cost is large, the reaction process is slow, the crystallization time is long, and the production efficiency is low. Therefore, we propose a processing method of glycine fermentation liquor and application thereof in fertilizer preparation. SUMMARY

[0004] The purpose of the present application is to provide a processing method of glycine fermentation liquor and application thereof in fertilizer preparation.

[0005] To solve the problems in the background art, the present application provides the following technical solution: a processing method of glycine fermentation liquor, comprising the following steps:

[0006] Step one, introduce the glycine fermentation liquor into a corrosion-resistant and well-insulated treatment container. First, perform preliminary detection on the fermentation liquor to determine its initial components, concentration and pH value parameters. Adjust the pH value of the fermentation liquor. Then, add 5% of the fermentation liquor volume of biological enzymes. The biological enzymes are extracted from various microbial fermentation, and the active ingredients include glucose oxidase, protease K and pectinase. The addition process needs to be slow and continuous stirring, and the stirring speed is adjusted to 60 r / min to ensure uniform mixing.

[0007] Step two, adjust the temperature of the processing container to 40℃, start the stirring device, continuously stir at a speed of 60r / min for 3h, so as to fully degrade the complex organic matter in the fermentation liquor, and in the process, the temperature change is monitored in real time, if the temperature fluctuation exceeds ±1℃, the heating and cooling device is adjusted in time to ensure the stability of the reaction temperature;

[0008] Step three, add the adsorbing material with high selective adsorption capacity, the amount of which is 20g per liter of fermentation liquor, the adsorbing material is composed of nanomaterials and has high selective adsorption capacity for glycine, after adding the adsorbing material, the stirring device is used for stirring.

[0009] Step four, control the stirring speed to be 60r / min, continue to stir for 1h, then filter through the filtering device to separate the adsorbing material and the fermentation liquor, when excessive foam appears in the last 10min of stirring, add the defoaming agent to the fermentation liquor, the single addition amount is 0.05ml, until the height of the foam does not exceed 10% of the height of the fermentation liquor, the amount of the defoaming agent is not more than 0.4ml per liter of fermentation liquor, then stand for a period of time.

[0010] Step five, after standing, the adsorbing material fully adsorbs glycine, then the material adsorbing glycine is separated through the multi-stage filtering device to separate the adsorbing material and the fermentation liquor, in the filtering process, the pressure is ensured to be stable at 0.2MPa-0.3MPa to avoid damage to the adsorbing material, then the fermentation liquor is placed in a microwave field for crystallization treatment to obtain glycine crystals.

[0011] As a further scheme of the application, in step one, before adding the biological enzyme, when the pH value of the acid-base degree is not in the range of 6.8-7.2, the acid-base degree of the fermentation liquor is adjusted to 6.8-7.2 which is the strongest activity of the biological enzyme by using an acid-base regulator, the acid-base regulator is selected from a 10% sodium hydroxide solution and an 8% hydrochloric acid solution, in the adjustment process, the acid-base regulator is slowly added and continuously stirred, the stirring speed is 40r / min, and the stirring time is 30min.

[0012] As a further scheme of the application, in step three, the adsorbing material is a material composed of nanosilica and carbon nanotubes, after adding the adsorbing material, the stirring speed is increased to 120r / min, and the continuous stirring time is 30min.

[0013] As a further scheme of the application, in step four, after continuously stirring for 1h, the multi-stage filtering device is used for separation to ensure that the adsorbing material and the fermentation liquor are completely separated, and the pore sizes of the filtering device are 50μm, 20μm and 5μm in sequence.

[0014] As a further scheme of the present application: in the step four, during the stirring process, the state of the fermentation liquor should be observed, if the foam exceeds 10% of the height of the fermentation liquor, then add a defoaming agent, the defoaming agent is selected from polyether defoaming agents, the amount of the defoaming agent is not more than 0.4 ml per liter of the fermentation liquor, after uniform stirring, the standing time should be adjusted according to the concentration and temperature of the fermentation liquor, when the concentration of the fermentation liquor is 12%-15%, the standing time is 2.5 h, when the concentration of the fermentation liquor is 10%-12%, the standing time is 2 h, and when the concentration of the fermentation liquor is less than 10%, the standing time is 1.5 h.

[0015] As a further scheme of the present application: in the step five, a microwave-assisted processing technology is used for the crystallization treatment, the microwave frequency is 2450 MHz, and the power is 500 W, in the crystallization step, the fermentation liquor after the adsorption treatment is placed in a microwave field, and irradiation is continued for 20 min to accelerate the formation of glycine crystals, during the microwave treatment, once the temperature exceeds 60℃, the microwave power is immediately reduced to 70% of the initial power, and when the temperature is reduced to 45℃-55℃, the irradiation is continued.

[0016] As a further scheme of the present application: in the step five, before the microwave treatment, the fermentation liquor is preheated to 45℃ to improve the efficiency of the microwave treatment, a rotary microwave reactor is used, so that the fermentation liquor is constantly rolled during the treatment process, the rotation speed is 30 r / min, after the microwave treatment is completed, rapid cooling is performed, the cooling method is circulating water cooling, and the temperature is cooled to below 30℃ to prevent the glycine crystals from being redissolved.

[0017] As a further scheme of the present application: in the step one, when the impurity concentration in the fermentation liquor exceeds 70%, the amount of the biological enzyme is increased to 7% of the volume of the fermentation liquor.

[0018] In addition, the present application also provides an application of the glycine fermentation liquor in the preparation of fertilizers, and the application of the glycine fermentation liquor in the preparation of fertilizers comprises the following specific steps:

[0019] Step one, the fermentation liquor containing glycine crystals is mixed with other fertilizer components, the mixing process is carried out in a stirring container, and the stirring speed is 40 r / min to ensure that the components are fully and uniformly mixed;

[0020] Step two, during the mixing process, the proportion of the glycine fermentation liquor and other fertilizer components is adjusted according to different fertilizer requirements, for high-nitrogen fertilizers, the proportion of the glycine fermentation liquor is reduced to 20%-30% of the total weight, and for fertilizers rich in trace elements, the proportion of the glycine fermentation liquor is increased to 40%-50% of the total weight.

[0021] Step three, after mixing, the fertilizer is granulated, the granulation process uses granulation equipment to make the fertilizer mixture into granules, the particle size is 2mm-4mm, in the process of fertilizer preparation, the analyzer is used to detect the glycine content, nutrient element ratio and impurity content parameters in the fertilizer, to ensure that the fertilizer meets the quality standards, if the test results do not meet the standards, adjust the proportion of fertilizer ingredients and reprocess;

[0022] Step four, the prepared fertilizer is packaged, the packaging material has moisture-proof, sun-proof and breathable properties, which ensures the quality and shelf life of the fertilizer, and the composition, use method and precautions of the fertilizer are clearly marked on the package, which facilitates the correct use of the fertilizer by users.

[0023] By using the above technical scheme, compared with the prior art, the beneficial effects of the present application are as follows:

[0024] 1. The present application introduces a biological enzyme assisted separation technology extracted by fermentation of multiple microorganisms, combined with an adsorbent material with high selective adsorption capacity, to realize efficient separation and resource recovery of each component in the glycine fermentation broth. The biological enzyme can selectively degrade complex organic matter in the fermentation broth, making it easier to separate. The adsorbent material can selectively and efficiently adsorb glycine, reducing the use of chemical reagents and significantly reducing environmental pollution. This method not only effectively improves the recovery rate of glycine, but also significantly reduces the emission of pollutants.

[0025] 2. The present application reasonably uses a defoaming agent in the treatment process of the glycine fermentation broth. When there is too much foam in the fermentation broth, adding a polyether defoaming agent can effectively eliminate the foam and avoid the adverse effects of foam on the normal operation of the stirring device and the subsequent processing steps. Such control can ensure effective defoaming while avoiding foam occupying the volume of the fermentation broth, reducing the amount of fermentation broth actually participating in the reaction and affecting the treatment effect. Excessive foam can increase the surface tension of the fermentation broth, leading to uneven stirring and affecting the transfer of substances and the progress of the reaction. The use of a defoaming agent can improve the stability and reliability of the treatment process, ensure the consistency of the treatment effect, and thus improve the product quality of glycine.

[0026] 3. The present application uses the unique properties of microwaves to accelerate the reaction process, thereby reducing energy consumption and time cost in the treatment process. In the treatment process of the glycine fermentation broth, microwaves can quickly excite molecular motion in the fermentation broth, promoting the reaction, especially in the crystallization step, which can significantly accelerate the formation of glycine crystals, greatly shortening the crystallization time. At the same time, microwave heating is fast and uniform, which can effectively reduce the overall energy consumption and significantly improve the production efficiency and reduce the cost. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 The extraction method steps in the embodiment of the present application are shown in the following schematic diagram.

[0028] Figure 2 The embodiment comparison schematic diagram in the embodiment of the present application is shown in the following. DETAILED DESCRIPTION

[0029] The specific embodiments of the present application are further described below in conjunction with the accompanying drawings, and it should be noted that the description of these embodiments is used to help understand the present application, but does not constitute a limitation on the present application.

[0030] In addition, the technical features involved in each of the embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.

[0031] Please refer to the accompanying Figure 1 - the accompanying Figure 2 The present application is a glycine fermentation broth treatment method, which comprises the following steps:

[0032] Step one, introduce the glycine fermentation broth into a corrosion-resistant and well-insulated treatment container, first conduct preliminary detection on the fermentation broth to determine its initial composition, concentration and pH value parameters, adjust the pH value of the fermentation broth, then add biological enzymes at 5% of the fermentation broth volume, the biological enzymes are extracted from various microbial fermentation, the active ingredients include glucose oxidase, protease K and pectinase, the addition process needs to be slow and continuously stirred, the stirring speed is adjusted to 60r / min to ensure uniform mixing;

[0033] Step two, adjust the temperature of the treatment container to 40℃, start the stirring device, continuously stir and react at a speed of 60r / min for 3h to fully degrade the complex organic matter in the fermentation broth, in this process, the temperature change needs to be monitored in real time, if the temperature fluctuation exceeds ±1℃, adjust the heating and cooling device in time to ensure the stability of the reaction temperature;

[0034] Step three, add an adsorption material with high selective adsorption capacity, the dosage is 20 grams per liter of fermentation broth, the adsorption material is composed of nanomaterials and has high adsorption selectivity for glycine, after adding the adsorption material, use the stirring device to stir;

[0035] Step four, control the stirring speed to 60r / min and continue to stir for 1h, then filter through a filter device to separate the adsorption material and the fermentation broth, when the last 10 minutes of stirring, observe the state of the fermentation broth, when there is too much foam, add a defoaming agent to the fermentation broth, the single addition amount is 0.05ml, until the height of the foam does not exceed 10% of the height of the fermentation broth, the dosage is not more than 0.4ml per liter of fermentation broth, then stand for a period of time;

[0036] Step five, after the standing is completed, the adsorption material is allowed to fully adsorb glycine, and then the material adsorbing glycine is separated by a multi-stage filtering device to separate the adsorption material and the fermentation liquor, and the pressure is ensured to be stable at 0.2-0.3 MPa during the filtering process to avoid damage to the adsorption material, and then the fermentation liquor is placed in a microwave field for crystallization treatment to obtain glycine crystals.

[0037] In an embodiment of the present application: in step one, before the biological enzyme is added, when the pH value of the acid-base degree is not within the range of 6.8-7.2, an acid-base regulator is used to adjust the acid-base degree of the fermentation liquor to 6.8-7.2, which is the strongest activity of the biological enzyme, the acid-base regulator is selected from a 10% sodium hydroxide solution and an 8% hydrochloric acid solution, and during the adjustment process, the acid-base regulator is slowly added and continuously stirred at a stirring speed of 40 r / min for 30 min.

[0038] In an embodiment of the present application: in step three, the adsorption material is a material composed of nano-silicon dioxide and carbon nanotubes, and after the adsorption material is added, the stirring speed is increased to 120 r / min and the stirring is continued for 30 min.

[0039] In an embodiment of the present application: in step four, after the stirring is continued for 1 h, a multi-stage filtering device is used for separation to ensure that the adsorption material and the fermentation liquor are completely separated, and the pore sizes of the filtering device are 50 μm, 20 μm and 5 μm in sequence.

[0040] In an embodiment of the present application: in step four, during the stirring process, the state of the fermentation liquor should be observed, if the foam exceeds 10% of the liquid level of the fermentation liquor, a defoaming agent is added, the defoaming agent is selected from a polyether defoaming agent, the amount of the defoaming agent is not more than 0.4 ml per liter of the fermentation liquor, after uniform stirring, the standing time should be adjusted according to the concentration and temperature of the fermentation liquor, when the concentration of the fermentation liquor is 12%-15%, the standing time is 2.5 h, when the concentration of the fermentation liquor is 10%-12%, the standing time is 2 h, and when the concentration of the fermentation liquor is less than 10%, the standing time is 1.5 h.

[0041] In an embodiment of the present application: in step five, a microwave-assisted treatment process is used for crystallization treatment, the microwave frequency is 2450 MHz, and the power is 500 W, in the crystallization step, the fermentation liquor after the adsorption treatment is placed in a microwave field, and the irradiation is continued for 20 min to accelerate the formation of glycine crystals, during the microwave treatment process, once the temperature exceeds 60℃, the microwave power is immediately reduced to 70% of the initial power, and when the temperature drops to 45-55℃, the irradiation is continued.

[0042] In one embodiment of the present application: in step five, the fermentation broth is preheated to 45℃ before microwave treatment to improve the efficiency of microwave treatment. A rotating microwave reactor is used to make the fermentation broth roll constantly during the treatment process at a rotation speed of 30r / min. After microwave treatment, rapid cooling is performed using circulating water cooling to a temperature below 30℃ to prevent the glycine crystals from redissolving.

[0043] In one embodiment of the present application: in step one, when the impurity concentration in the fermentation broth exceeds 70%, the amount of biological enzyme is increased to 7% of the volume of the fermentation broth.

[0044] An application of a glycine fermentation broth in fertilizer preparation, the application of the glycine fermentation broth in fertilizer preparation includes the following specific steps:

[0045] Step one, mix the fermentation broth containing glycine crystals with other fertilizer ingredients in a stirring container at a stirring speed of 40r / min to ensure that the ingredients are fully mixed and uniform;

[0046] Step two, during the mixing process, adjust the proportion of the glycine fermentation broth and other fertilizer ingredients according to different fertilizer requirements. For high-nitrogen fertilizers, reduce the proportion of the glycine fermentation broth to 20%-30% of the total weight. For fertilizers rich in trace elements, increase the proportion of the glycine fermentation broth to 40%-50% of the total weight.

[0047] Step three, after mixing, the fertilizer is granulated using granulation equipment to make the fertilizer mixture into granules with a size of 2mm-4mm. During the preparation of the fertilizer, analytical instruments are used to detect the glycine content, nutrient element proportion, and impurity content parameters in the fertilizer to ensure that the fertilizer meets the quality standards. If the test results do not meet the standards, adjust the proportion of the fertilizer ingredients and reprocess them.

[0048] Step four, package the prepared fertilizer using packaging materials with moisture-proof, sun-proof, and breathable properties to ensure the quality and shelf life of the fertilizer. The packaging clearly indicates the composition, usage method, and precautions of the fertilizer to facilitate the correct use of the fertilizer by users.

[0049] Example one, please refer to the attached Figure 1 -attached Figure 2The glycine fermentation liquor is introduced into a corrosion-resistant and heat-retaining treatment container, and preliminary detection is performed on the fermentation liquor to determine its initial components, concentration and pH value. When the pH value is not within the range of 6.8-7.2, 10% sodium hydroxide solution and 8% hydrochloric acid solution are used as the acid-base regulator, and are slowly added while being continuously stirred at a speed of 40 r / min for 30 min. The pH value of the fermentation liquor is adjusted to 6.8-7.2. Then, the biological enzyme is slowly added at 5% of the volume of the fermentation liquor. When the impurity concentration in the fermentation liquor exceeds 70%, the amount of the biological enzyme is increased to 7% of the volume of the fermentation liquor. The biological enzyme is extracted from a plurality of microorganisms, and the active ingredients include glucose oxidase, protease K and pectinase. During the adding process, the stirring speed is continuously increased to 60 r / min to ensure uniform mixing. Then, the temperature of the treatment container is adjusted to 40℃, and the stirring device is started to continuously stir and react at a speed of 60 r / min for 3 h, so that the complex organic matter in the fermentation liquor is fully degraded. During this process, if the temperature fluctuation exceeds ±1℃, the heating and cooling devices are adjusted in time to ensure that the reaction temperature is stable at 40℃. Then, the adsorption material, which is a material composed of nano-silicon dioxide and carbon nanotubes, is added at a dosage of 20 g per liter of fermentation liquor. After the addition, the stirring speed is increased to 120 r / min and continuously stirred for 30 min. Then, the stirring speed is controlled to 60 r / min and continuously stirred for 1 h. During the last 10 min of stirring, the state of the fermentation liquor is observed. If the foam exceeds 10% of the height of the fermentation liquor, polyether defoaming agent is added to the fermentation liquor, and the single addition amount is 0.05 ml. The defoaming agent is added until the height of the foam does not exceed 10% of the height of the fermentation liquor, and the dosage of the defoaming agent is not more than 0.4 ml per liter of fermentation liquor. After continuously stirring for 1 h, a multi-stage filtration device with pore sizes of 50 μm, 20 μm and 5 μm is used for separation to ensure that the adsorption material and the fermentation liquor are completely separated. After uniform stirring, the standing time is adjusted according to the concentration of the fermentation liquor. When the concentration of the fermentation liquor is 12%-15%, the standing time is 2.5 h. When the concentration of the fermentation liquor is 10%-12%, the standing time is 2 h. When the concentration of the fermentation liquor is less than 10%, the standing time is 1.5 h. After standing, the adsorption material fully adsorbs the glycine. Then, the material adsorbed with glycine is separated through the multi-stage filtration device. During the filtration process, the pressure is ensured to be stable at 0.2MPa, to avoid damage to the adsorbent material, then the fermentation broth is placed in the microwave field for crystallization treatment, using a microwave-assisted treatment process, the microwave frequency is 2450MHz, the power is 500W, during the crystallization process, the fermentation broth after adsorption treatment is placed in the microwave field, and the irradiation is continued for 20min, before microwave treatment, the fermentation broth is preheated to 45℃, a rotary microwave reactor is used to make the fermentation broth continuously roll during the treatment process, the rotation speed is 30r / min, during the microwave treatment process, a temperature sensor is used to monitor the temperature of the fermentation broth in real time, once the temperature exceeds 60℃, the microwave power is immediately reduced to 70% of the initial power, and when the temperature drops to 50℃, the irradiation is continued, after the microwave treatment is completed, rapid circulating water cooling is carried out, and the temperature is cooled to below 30℃, to prevent the glycine crystals from redissolving, and finally the glycine crystals are obtained.

[0050] Example two, please refer to the attached Figure 1 -attached Figure 2 The glycine fermentation broth is introduced into a common treatment container, the stirring device is turned on, and stirring is continuously carried out at a speed of 50r / min for 2.5h, then activated carbon is added as the adsorbent material, the amount is 15g per liter of fermentation broth, after adding, the stirring speed is increased to 100r / min, and stirring is continuously carried out for 20min, then the stirring speed is controlled to 50r / min, and stirring is continuously carried out for 0.5h, after 0.5h of continuous stirring, a multi-stage filtration device with pore sizes of 60μm, 30μm and 8μm is used for separation, after stirring, the standing time is adjusted according to the concentration of the fermentation broth, when the fermentation broth concentration is 12%-15%, the standing time is 2h, when the fermentation broth concentration is 10%-12%, the standing time is 1.5h, and when the fermentation broth concentration is less than 10%, the standing time is 1h, after standing is completed, the material adsorbed with glycine is separated through the filtration device, then the treated fermentation broth is simply concentrated by heating to 60℃, and evaporating part of the water to increase the glycine concentration.

[0051] Example three, please refer to the attached Figure 1 -attached Figure 2The glycine fermentation liquor is introduced into a corrosion-resistant and heat-retaining treatment container, and preliminary detection is performed on the fermentation liquor to determine its initial components, concentration, and pH value; when the pH value is not within the range of 6.8-7.2, 10% sodium hydroxide solution and 8% sulfuric acid solution are used as acid-base regulators, slowly added, and continuously stirred at a speed of 40 r / min for 30 min, and the pH value of the fermentation liquor is adjusted to 6.8-7.2; then, 5% of the volume of the fermentation liquor is slowly added as a biological enzyme; when the impurity concentration in the fermentation liquor exceeds 70%, the amount of the biological enzyme is increased to 7% of the volume of the fermentation liquor; the biological enzyme is extracted from a plurality of ordinary microorganisms, and active ingredients include glucose oxidase and protease; during the adding process, continuous stirring is performed at a speed of 60 r / min to ensure uniform mixing; then, the temperature of the treatment container is adjusted to 40℃, the stirring device is turned on, continuous stirring is performed at a speed of 60 r / min, and reaction is performed for 3 h, so that complex organic matter in the fermentation liquor is fully degraded; during the process, if the temperature fluctuation exceeds ±1℃, the heating and cooling devices are adjusted in a timely manner to ensure that the reaction temperature is stable at 40℃; then, an adsorption material is added, the material is a material composed of nano-aluminum oxide and carbon nanofiber, and the amount of the material is 20 g per liter of the fermentation liquor; after the material is added, the stirring speed is increased to 120 r / min, and continuous stirring is performed for 30 min; then, the stirring speed is controlled to 60 r / min, and continuous stirring is performed for 1 h; when stirring is performed for the last 10 min, the state of the fermentation liquor is observed; if the foam exceeds 10% of the height of the fermentation liquor, polyether defoaming agent is added to the fermentation liquor, and the amount of the defoaming agent added at a time is 0.05 ml; the height of the foam does not exceed 10% of the height of the fermentation liquor until the amount of the defoaming agent used is not more than 0.4 ml per liter of the fermentation liquor; after continuous stirring is performed for 1 h, a multi-stage filtration device with pore sizes of 50 μm, 20 μm, and 5 μm is used for separation to ensure that the adsorption material and the fermentation liquor are completely separated; after stirring is uniformly performed, the standing time is adjusted according to the concentration of the fermentation liquor; when the concentration of the fermentation liquor is 12%-15%, the standing time is 2.5 h; when the concentration of the fermentation liquor is 10%-12%, the standing time is 2 h; when the concentration of the fermentation liquor is less than 10%, the standing time is 1.5 h; after standing is completed, the adsorption material fully adsorbs the glycine; then, the material adsorbing the glycine is separated through the multi-stage filtration device; during the filtration process, the pressure is ensured to be stable at 0.2-0.3 MPa to avoid damage to the adsorption material; subsequently, the treated fermentation liquor is subjected to simple concentration treatment, heated to 80℃, and part of the water is evaporated.

[0052] A plurality of liters of glycine fermentation liquor of the same source and batch are taken to ensure that the initial components, concentration, and pH value are basically consistent;

[0053] An analytical instrument for detecting the concentration and purity of glycine is prepared;

[0054] The glycine fermentation broth was divided into three equal parts and processed according to the methods of Example 1, Example 2 and Example 3 respectively. Each example was repeated three times.

[0055] During the processing, detailed energy consumption data extracted for each embodiment is recorded, including:

[0056] Electrical energy consumed during the heating process (unit: kilowatt-hours);

[0057] Electrical energy consumed during the stirring process (unit: kilowatt-hours);

[0058] Electrical energy consumed during the cooling process (unit: kilowatt-hours);

[0059] Electrical energy consumed by other related equipment (unit: kilowatt-hours);

[0060] After processing, the mass (in grams) and purity (%) of the glycine crystals obtained in each example were determined using analytical instruments such as high performance liquid chromatography, and the recovery efficiency (%) of glycine was calculated based on the glycine content in the initial fermentation broth.

[0061] The experimental results were statistically analyzed to calculate the average glycine recovery efficiency, average product purity, and average extraction energy consumption for each embodiment, and the advantages and disadvantages of the three embodiments were compared.

[0062] As attached Figure 2 As shown in the table, it can be seen that Example 1 is significantly better than Example 2 and Example 3 in terms of glycine recovery efficiency and product quality, and also has lower extraction energy consumption. This indicates that Example 1 has higher glycine recovery efficiency, better product quality, and can reduce extraction energy consumption, improve extraction efficiency, and reduce costs.

[0063] Furthermore, this application uses specific terms to describe its embodiments. For example, "one embodiment," "an embodiment," and / or "some embodiments" refer to a particular feature, structure, or characteristic associated with at least one embodiment of this application. Therefore, it should be emphasized and noted that "one embodiment," "an embodiment," or "an alternative embodiment" mentioned twice or more in different locations in this specification do not necessarily refer to the same embodiment. In addition, certain features, structures, or characteristics in one or more embodiments of this application can be appropriately combined.

[0064] For simplicity and to help with the understanding of one or more implementations of the application, the description of implementations of the application sometimes uses terms like "one implementation," "another implementation," "an implementation," and "some implementations" to describe various features. However, the use of these terms does not limit the application to the features described in the implementations using these terms. Rather, these terms have been used as shorthand notations for a single implementation or a combination of different implementations described throughout the detailed description of implementations of the application. The features described in the various implementations using these terms can be combined with each other to form implementations of the application. As used herein, "about" means approximately or nearly and in the context of a numerical value or range of values means ±20% of the numerical value or range of values. Accordingly, numerical values used in the description and claims of the application are approximations that can vary depending upon the desired properties sought to be obtained by the individual implementation. In some implementations, numerical values used in the description and claims of the application are approximations that will vary from the numerical values stated in the specification and claims due to, for example, variations in manufacturing tolerances, and errors in measuring and testing equipment. In some implementations, numerical values used in the description and claims of the application are approximations that will vary from the numerical values stated in the specification and claims due to, for example, variations in manufacturing tolerances, and errors in measuring and testing equipment. Unless otherwise stated, "about" or "approximate" shall mean ±20% of the stated value. In some implementations, numerical values used in the description and claims of the application are approximations that will vary from the numerical values stated in the specification and claims due to, for example, variations in manufacturing tolerances, and errors in measuring and testing equipment. In some implementations, numerical values used in the description and claims of the application are approximations that will vary from the numerical values stated in the specification and claims due to, for example, variations in manufacturing tolerances, and errors in measuring and testing equipment. In some implementations, numerical values used in the description and claims of the application are approximations that will vary from the numerical values stated in the specification and claims due to, for example, variations in manufacturing tolerances, and errors in measuring and testing equipment.

[0065] Although the application has been disclosed in connection with the preferred embodiments shown, it should be understood that many modifications, variations and alterations to the application are possible in light of the disclosure herein. It is therefore contemplated to cover the possibilities defined by the scope of the appended claims and their equivalents.

Claims

1. A method for treating a glycine fermentation broth, characterized by, The processing method comprises the following steps: Step one, the glycine fermentation broth is introduced into a corrosion-resistant and well-insulated treatment container, the fermentation broth is first detected to determine its initial composition, concentration and pH value, the pH value of the fermentation broth is adjusted, then 5% of the volume of the fermentation broth is added with biological enzymes, the biological enzymes are extracted from various microbial fermentation, the active ingredients include glucose oxidase, protease K and pectinase, the addition process needs to be slow and continuous stirring, the stirring speed is adjusted to 60r / min to ensure uniform mixing; Step two, the temperature of the treatment container is adjusted to 40℃, the stirring device is turned on, the stirring is continuously carried out at a speed of 60r / min for 3h to fully degrade the complex organic matter in the fermentation broth, in this process, the temperature change needs to be monitored in real time, if the temperature fluctuation exceeds ±1℃, the heating and cooling devices need to be adjusted in time to ensure the stability of the reaction temperature; Step three, an adsorbent material with high selective adsorption capacity is added, the dosage is 20g per liter of fermentation broth, the adsorbent material is composed of nanomaterials and has high adsorption selectivity for glycine, after adding the adsorbent material, the stirring device is used for stirring; In step three, the adsorbent material is a material composed of nanosilica and carbon nanotubes, after adding the adsorbent material, the stirring speed is increased to 120r / min and the stirring is continued for 30min; Step four, the stirring speed is controlled to 60r / min and the stirring is continued for 1h, then the fermentation broth is filtered by a filtering device to separate the adsorbent material and the fermentation broth, when the stirring is completed for the last 10min, the state of the fermentation broth is observed, when excessive foam appears, a defoaming agent is added to the fermentation broth, the single addition amount is 0.05ml, until the height of the foam does not exceed 10% of the height of the fermentation broth, the dosage is not more than 0.4ml per liter of fermentation broth, then the fermentation broth is left to stand for a period of time; Step five, after the standing is completed, the adsorbent material fully adsorbs glycine, then the material adsorbed with glycine is separated out by a multi-stage filtering device to separate the adsorbent material and the fermentation broth, the pressure is ensured to be stable at 0.2MPa-0.3MPa during the filtering process to avoid damage to the adsorbent material, then the fermentation broth is placed in a microwave field for crystallization treatment to obtain glycine crystals.

2. The method of claim 1, wherein the glycine fermentation broth is treated by: In step one, before adding the biological enzymes, when the pH value of the acid-base degree is not within the range of 6.8-7.2, an acid-base regulator is used to adjust the acid-base degree of the fermentation broth to 6.8-7.2, which is the strongest activity range of the biological enzymes, the acid-base regulator is selected from a 10% sodium hydroxide solution and an 8% hydrochloric acid solution, during the adjustment process, the acid-base regulator is slowly added and continuously stirred, the stirring speed is 40r / min and the stirring time is 30min.

3. The method of claim 1, wherein the glycine fermentation broth is treated by: In step four, after the stirring is continued for 1h, a multi-stage filtering device is used for separation to ensure that the adsorbent material and the fermentation broth are completely separated, the pore sizes of the filtering device are 50μm, 20μm and 5μm in sequence.

4. The method of claim 1, wherein the glycine fermentation broth is treated by a method comprising: In the fourth step, attention should be paid to the state of the fermentation broth during stirring. If the foam exceeds 10% of the liquid level, a defoaming agent should be added. The defoaming agent is selected from polyether defoaming agents, and the dosage of the defoaming agent should not exceed 0.4 ml per liter of fermentation broth. After uniform stirring, the standing time should be adjusted according to the concentration and temperature of the fermentation broth. When the concentration of the fermentation broth is 12%-15%, the standing time is 2.5 hours. When the concentration of the fermentation broth is 10%-12%, the standing time is 2 hours. When the concentration of the fermentation broth is below 10%, the standing time is 1.5 hours. ​ 5. The method of claim 1, wherein the glycine fermentation broth is treated by a method comprising: In the fifth step, a microwave-assisted treatment process is used for crystallization treatment. The microwave frequency is 2450 MHz, and the power is 500 W. During the crystallization step, the fermentation broth after adsorption treatment is placed in a microwave field for continuous irradiation for 20 minutes to accelerate the formation of glycine crystals. During microwave treatment, if the temperature exceeds 60°C, the microwave power is immediately reduced to 70% of the initial power. When the temperature drops to 45°C-55°C, the irradiation is continued. ​ 6. The method of claim 1, wherein the glycine fermentation broth is treated by: In the fifth step, before microwave treatment, the fermentation broth is preheated to 45°C to improve the efficiency of microwave treatment. A rotary microwave reactor is used to make the fermentation broth roll continuously during the treatment process at a rotation speed of 30 r / min. After microwave treatment, rapid cooling is performed using circulating water cooling to cool the temperature to below 30°C to prevent the glycine crystals from redissolving.

7. The method of claim 1, wherein the glycine fermentation broth is treated by a method comprising: In the first step, when the impurity concentration in the fermentation broth exceeds 70%, the amount of biological enzyme is increased to 7% of the volume of the fermentation broth. ​ 8. Use of a glycine fermentation broth suitable for the process according to any one of claims 1 to 7 for the production of fertilizers, characterized in that, The application of the glycine fermentation broth in fertilizer preparation includes the following specific steps: Step one, mix the fermentation broth containing glycine crystals with other fertilizer ingredients. The mixing process is carried out in a stirring container at a stirring speed of 40 r / min to ensure that the ingredients are fully mixed and uniform. Step two, during the mixing process, adjust the ratio of glycine fermentation broth to other fertilizer ingredients according to different fertilizer requirements. For high-nitrogen fertilizers, reduce the proportion of glycine fermentation broth to 20%-30% of the total weight. For fertilizers rich in trace elements, increase the proportion of glycine fermentation broth to 40%-50% of the total weight. Step three, after mixing, the fertilizer is granulated using granulation equipment to make the fertilizer mixture into granules with a size of 2 mm-4 mm. During the preparation of the fertilizer, analytical instruments are used to detect the glycine content, nutrient element ratio, and impurity content parameters in the fertilizer to ensure that the fertilizer meets the quality standards. If the test results do not meet the standards, adjust the proportion of the fertilizer ingredients and reprocess them. Step four, package the prepared fertilizer. The packaging material has moisture-proof, sun-proof, and breathable properties to ensure the quality and shelf life of the fertilizer. The packaging clearly marks the composition, usage method, and precautions of the fertilizer to facilitate the correct use of the fertilizer by users.

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