Organic bacterial fertilizer for tea trees and preparation method of organic bacterial fertilizer

Abstract

The invention relates to the field of microbial fertilizers, and discloses an organic bacterial fertilizer for tea trees and a preparation method thereof. The preparation method disclosed by the invention comprises the following steps: S1, mixing bacterial solutions after reproduction of streptococcus faecalis, photosynthetic bacteria, bacillus, cellulose decomposition bacteria, saccharomycetes and lactic acid bacteria to obtain a probiotic flora solution; s2, inoculating pig manure with the probiotic flora solution, and uniformly stirring to obtain bacterial manure; and S3, inoculating bacterial liquid obtained after reproduction of photosynthetic cells, lactic acid bacteria, saccharomycetes and cellulose-decomposing bacteria into bacterial manure, and uniformly stirring to obtain the organic bacterial manure for tea trees. The content of nitrite in the organic bacterial fertilizer is not larger than 0.2 mg / dm < 3 >, the content of ammoniacal nitrogen is not larger than 5%, the yield increase range of tea trees using the organic bacterial fertilizer reaches 51%, and the prepared kungfu tea shows the excellent quality of thick green and smooth appearance, rich fragrance, golden yellow and transparent liquor color, soft taste and sweet aftertaste.

Description

Technical Field

[0001] The invention relates to the field of microbial fertilizers, and more specifically, to an organic bacterial fertilizer for tea trees and a preparation method thereof. Background Art

[0002] my country has entered a period of high growth and high economy. Pork and tea are the most important daily consumer goods for the people. The quality of tea and the pollution caused by pig farming are issues that the current government attaches great importance to, and are also important research topics for agricultural experts. The long-term use of chemical fertilizers has caused the soil fertility of tea gardens in many areas to decline, soil compaction is serious, the tea trees have poor resistance to pests, and the quality of tea has become lower year by year.

[0003] Although traditional organic fertilizers can improve the quality of tea and improve soil compaction, they contain excessive nitrite and a large number of molds, anaerobic bacteria and other harmful bacteria that can cause organic matter to decay. The use of organic fertilizers that have not been steamed or fermented at high temperatures will increase the number of pests and diseases in tea gardens, affecting the yield and beauty of tea. Steaming or high-temperature fermentation of organic fertilizers will cause secondary pollution to the environment and reduce the content of ammonia nitrogen and probiotics in organic fertilizers. The fertilizer effect is not long-lasting, and the improvement of tea quality and yield is still restricted.

[0004] In recent years, new organic fertilizers have been promoted as an improvement plan. Compared with traditional fertilizers, organic fertilizers have shown better effects in improving fertilizer efficiency and improving soil. However, most organic fertilizers use cow dung as the main raw material, while fertilizers based on pig dung are relatively rare, and special organic fertilizers for the special needs of tea trees have hardly been widely used.

[0005] It is worth noting that pig manure has unique advantages as a high-quality raw material for organic fertilizer. Due to the short digestive system of pigs, a higher proportion (about 70%) of organic matter is retained in their feces, which makes pig manure an ideal basic material for making organic fertilizer. However, in reality, pig manure is often mixed with urine, resulting in a high moisture content, which is easy to ferment and deteriorate and breed harmful bacteria, produce odor, and cause environmental pollution. This phenomenon not only increases the processing cost of the farm, but also becomes a major problem for farmers.

[0006] The prior art CN103708967A uses pig manure to produce organic fertilizer. After the pig manure is separated into dry and wet parts, it is fermented dynamically and continuously at high temperature using EM mixed bacterial agents to decompose and transform the easily decomposable and odor-producing substances in the pig manure, and the generated high temperature is used to kill pathogenic bacteria. However, the technology uses a single strain of bacteria, the organic substances in the pig manure cannot be completely decomposed, and the nutrient utilization rate is not high.

[0007] The prior art CN104388350B prepares a composite microbial agent from fermented liquid of Bacillus subtilis, Bacillus megaterium, photosynthetic bacteria, actinomycetes, yeast, lactic acid bacteria and Streptococcus faecalis to increase the dissolved oxygen content of water and inhibit the growth of harmful pathogens and algae. However, the composite microbial agent cannot be applied to the planting industry to improve the quality of soil and crops. Summary of the invention

[0008] The present invention provides an organic bacterial fertilizer for tea trees to overcome the defects of the prior art, such as single bacterial species, inability to completely decompose organic matter in pig manure, low nutrient utilization rate, inability to be applied to planting industry, and failure to meet the growth requirements of tea trees.

[0009] Another object of the present invention is to provide a method for preparing tea tree organic bacterial fertilizer.

[0010] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0011] An organic bacterial fertilizer for tea trees, prepared from pig manure, fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast and lactic acid bacteria; the nitrite content of the organic bacterial fertilizer for tea trees is not more than 0.2 mg / dm 3 , ammonia nitrogen content is not more than 5%.

[0012] A method for preparing tea tree organic bacterial fertilizer comprises the following steps:

[0013] S1: Mixing the bacterial liquids of fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast and lactic acid bacteria to obtain a probiotic bacterial solution;

[0014] S2: inoculate the probiotic bacteria solution on the pig manure and stir evenly to obtain bacterial manure;

[0015] S3: Inoculate the bacterial liquid after reproduction of photosynthetic cells, lactic acid bacteria, yeast and fiber-decomposing bacteria into the bacterial feces, stir evenly, and obtain organic bacterial fertilizer for tea trees.

[0016] Furthermore, the bacterial liquid after propagation in S1 is prepared by the following steps:

[0017] S01: Inoculate fecal streptococci, photosynthetic bacteria, Bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria into the culture medium respectively;

[0018] S02: After 5 to 10 days of culture, take the culture medium and inoculate it into the newly prepared culture medium;

[0019] S03: Repeat S02 until the total culture time reaches 38 to 42 days to obtain the propagated bacterial liquid.

[0020] Preferably, the bacterial liquid after propagation in S1 is prepared by the following steps:

[0021] S01: Inoculate fecal streptococci, photosynthetic bacteria, Bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria into the culture medium respectively;

[0022] S02: After 7 days of culture, take the culture medium and inoculate it into the newly prepared culture medium;

[0023] S03: Repeat S02 until the total culture time reaches 40 days to obtain the propagated bacterial liquid.

[0024] Preferably, the culture temperature in S01-S03 is 20-45°C, and the culture is stirred 2-3 times a day.

[0025] Preferably, the total weight of the bacterial solution after expansion can reach 4000 times the total weight of the bacterial agents of fecal streptococci, photosynthetic bacteria, bacillus, cellulose-decomposing bacteria, yeast and lactic acid bacteria before expansion.

[0026] Furthermore, in S01, fecal streptococci, photosynthetic bacteria, bacillus, cellulose-decomposing bacteria, yeast, and lactic acid bacteria are inoculated into a culture medium at a mass ratio of 1:2 to 5; the culture medium is obtained by mixing water, sugar, flour, salt, urea, and potassium dihydrogen phosphate.

[0027] Preferably, in S01, fecal streptococci, photosynthetic bacteria, Bacillus, cellulose-decomposing bacteria, yeast, and lactic acid bacteria are inoculated into the culture medium at a mass ratio of 1:3.

[0028] Preferably, the culture medium is obtained by mixing water, sugar, flour, salt, urea and potassium dihydrogen phosphate in a mass ratio of 80-100:8-10:3-5:0.2-0.5:0.2-0.5:0.2-0.5.

[0029] Preferably, the culture medium is obtained by mixing water, sugar, flour, salt, urea and potassium dihydrogen phosphate in a mass ratio of 100:9:4:0.3:0.3:0.3.

[0030] Furthermore, in S1, bacterial liquids after reproduction of fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast and lactic acid bacteria are mixed in a mass ratio of 1-2:1-2:1-2:1-2:1-2:1-2:1-2 to obtain a probiotic bacterial solution.

[0031] Preferably, in S1, bacterial liquids after reproduction of fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast and lactic acid bacteria are mixed in a mass ratio of 1:1:1:1:1:1 to obtain a probiotic bacteria solution.

[0032] Furthermore, the mass ratio of the probiotic solution to pig manure in S2 is 1:5-15.

[0033] Preferably, the mass ratio of the probiotic solution to pig manure in S2 is 1:10.

[0034] Furthermore, S2 also includes the following steps: inoculating the probiotic bacteria solution on the pig manure and stirring evenly to obtain bacterial manure after 5 to 10 days; adding the pig manure to the bacterial manure at a volume ratio of 2 to 5:1, stirring evenly for 28 to 32 days, and the pig manure becomes bacterial manure.

[0035] Preferably, pig manure is added to bacterial manure in a volume ratio of 3:1.

[0036] Preferably, the culture temperature is 20-45°C.

[0037] Preferably, the S2 further comprises the following steps:

[0038] Inoculate the probiotic solution on the pig manure, turn the manure every 3 hours, and the odor of the pig manure will be eliminated after 24 hours; turn the pig manure once a day, and you will get bacterial manure after 5 to 10 days; add the pig manure to the bacterial manure at a volume ratio of 2 to 5:1, turn the pig manure every 3 hours, after 24 hours, turn the pig manure once a day again, and continue for 5 to 10 days; repeat the previous steps, and after 28 to 32 days, the pig manure will become bacterial manure.

[0039] Preferably, the weight ratio of the probiotic solution to the processable pig manure can reach 1:700.

[0040] Furthermore, the nitrite and aminopeptide nitrogen content of the bacterial feces obtained in S2 was tested, and the nitrite content in the bacterial feces was required to be no more than 0.2 mg / dm 3 , the ammonia nitrogen content must not exceed 5%.

[0041] Furthermore, the nitrite content in the feces is greater than 0.2 mg / dm 3 When the ammonia nitrogen content is greater than 5%, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulose-decomposing bacteria, and lactic acid bacteria are inoculated in a ratio of 2-3:2-3:2-3:2-3:1-1.5:1-1.5, according to the mass ratio of bacterial feces to probiotic bacteria solution of 280-320:1, and stirred evenly for 5-12 days.

[0042] Preferably, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulolytic bacteria, and lactic acid bacteria are inoculated in a ratio of 2:2:2:2:1:1, with a mass ratio of bacterial feces to probiotic bacteria solution of 300:1.

[0043] Preferably, stirring is performed once a day for 10 days after inoculation.

[0044] Furthermore, the photosynthetic cells, lactic acid bacteria, yeast, and fiber-decomposing bacteria in S3 are mixed in a mass ratio of 2.5-3:1-2:1-2:2.5-3, and after mixing, they are inoculated into the bacterial fertilizer in a mass ratio of 1:480-530.

[0045] Preferably, the photosynthetic cells, lactic acid bacteria, yeasts and fiber-decomposing bacteria in S3 are mixed in a mass ratio of 3:2:2:3, and after mixing, they are inoculated into the bacterial fertilizer in a mass ratio of 1:500.

[0046] Preferably, the manure is stirred once every 5 days after inoculation.

[0047] Preferably, when the moisture content of the bacterial feces is not more than 10%, it can be crushed or made into granules to become an organic probiotic fertilizer for tea trees.

[0048] The present invention innovatively adds probiotics such as fecal streptococci, photosynthetic bacteria, lactic acid bacteria, yeast, bacillus, and cellulolytic bacteria into pig manure, which can establish the advantages of the probiotic community in the pig manure, inhibit and eliminate harmful bacteria such as mold and anaerobic bacteria, and quickly decompose the organic matter in the pig manure and convert it into bacterial protein that helps to improve the immunity of animals and plants. It not only eliminates odors, but also turns the pig manure into soft humus. At the same time, the ammonia nitrogen in the pig manure is absorbed by the probiotic community, and a large amount of nutrients are also absorbed and retained.

[0049] Fecal streptococci and photosynthetic bacteria can reduce the concentration of nitrite in pig manure. When the concentration of nitrite in pig manure exceeds the standard, appropriately increasing the proportion of fecal streptococci and photosynthetic bacteria in the probiotic group can reduce the concentration of nitrite. When the ammonia nitrogen content in pig manure is too high, increasing the proportion of yeast and bacillus in the probiotic group can convert a large amount of nitrogen into the probiotics, thereby reducing the content of ammonia nitrogen.

[0050] Cellulose-decomposing bacteria and lactic acid bacteria help to increase the concentration of tea aromatic alcohol and amino acids, and improve the taste of tea. Yeast and photosynthetic bacteria can also effectively and permanently improve soil fertility and improve soil compaction. Therefore, if the proportion of lactic acid bacteria, yeast, cellulose-decomposing bacteria and photosynthetic bacteria in organic fertilizer is appropriately increased, probiotic organic fertilizer can become a special fertilizer for tea trees.

[0051] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0052] 1. Improve the growth environment of tea trees and promote the healthy development of the root system. The synergistic effect of fecal streptococci, lactic acid bacteria and bacillus enhances the microecological balance of the rhizosphere, allowing tea trees to absorb nutrients more efficiently. Photosynthetic bacteria inhibit harmful microorganisms such as anaerobic bacteria and molds, reduce pests and diseases, and improve the disease resistance of tea trees.

[0053] 2. Increase tea yield. The cellulose decomposing bacteria in the organic fertilizer promotes soil softness and aeration, increasing biomass accumulation. The yield of fresh tea leaves using the organic fertilizer of the present invention reaches 250kg / mu, and the yield of dry tea is 62.5kg / mu. Compared with the organic fertilizer of the traditional fermentation method, the yield increase is 51%.

[0054] 3. Improve the quality of tea. The effects of lactic acid bacteria and bacillus in organic fertilizer significantly increase the amino acid, polyphenol and aromatic alcohol content of tea, making the taste of tea smoother and more sweet. The Kung Fu tea made from tea leaves using the organic fertilizer of the present invention has excellent quality with thick green and smooth appearance, rich aroma, golden and bright soup color, and soft and sweet taste.

[0055] 4. Eliminate odor and reduce pollution. The synergistic effect of multiple strains can eliminate the odor of pig manure within 24 hours, reduce the harmful gases produced by anaerobic fermentation, and prevent environmental pollution. The nitrite content in the organic bacterial fertilizer of the present invention is not more than 0.2 mg / dm 3 , the ammonia nitrogen content is no more than 5%, which reduces the adverse effects on tea trees. DETAILED DESCRIPTION

[0056] The present invention is further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0057] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0058] Example 1

[0059] The first step is to prepare the culture medium according to the mass ratio of 100 catties of water, 9 catties of sugar, 4 catties of flour, 0.3 catties of salt, 0.3 catties of urea, and 0.3 catties of potassium dihydrogen phosphate.

[0060] The second step is to pour the bacterial agents of fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast, and lactic acid bacteria into the culture medium at a mass ratio of 1:3 at a temperature of 20 to 45 degrees Celsius for reproduction, and stir twice a day. After 7 days, continue to pour the cultured bacterial liquid into the newly prepared culture medium at a mass ratio of 1:3, and repeat this 5 times. After 40 days, one kilogram of bacterial agent can be made into about two tons of bacterial liquid.

[0061] The third step is to mix the expanded fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria in a ratio of 1:1:1:1:1:1 to obtain a probiotic solution, and spray it evenly on the pig manure at a mass ratio of 1:10. Turn the pig manure over every 3 hours to use the advantages of the probiotics to eliminate mold, anaerobic bacteria and other harmful bacteria in the pig manure. Generally, the odor of pig manure can be eliminated after 24 hours.

[0062] The fourth step is to control the temperature between 20 and 45 degrees Celsius, turn the pig manure once a day, and allow the probiotics to coexist with the odor-free pig manure to obtain bacterial manure.

[0063] Step 5: After 7 days, at the same temperature, add the pig manure to the bacterial manure at a volume ratio of 3:1 and stir evenly, turning it over every 3 hours for 8 times, and then once a day for 7 consecutive days. Repeat the above operation once more. After 30 days, 1 catties of probiotic solution can treat about 700 catties of pig manure.

[0064] The sixth step is testing. When the nitrite content in each cubic decimeter of manure is greater than 0.2 mg and the ammonia nitrogen content is greater than 5%, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulose decomposing bacteria, and lactic acid bacteria are sprayed with bacterial solution and stirred at a mass ratio of 2:2:2:2:1:1, and the manure to bacterial solution is 300:1, once a day for 10 consecutive days. By adjusting the ratio of various probiotics in the bacterial community, the content of nitrite and aminopeptide nitrogen in pig manure can be effectively controlled.

[0065] The seventh step is to spray and stir the manure with photosynthetic cells, lactic acid bacteria, yeast, and fiber-decomposing bacteria in a ratio of 3:2:2:3 and a mass ratio of manure to bacteria of 500:1, once every 5 days.

[0066] Step 8. When the humidity of the pig manure reaches 10%, crush it or make it into granules to become organic probiotic fertilizer for tea trees.

[0067] Example 2

[0068] The first step is to prepare the culture medium according to the mass ratio of 80 catties of water, 8 catties of sugar, 3 catties of flour, 0.2 catties of salt, 0.2 catties of urea, and 0.2 catties of potassium dihydrogen phosphate.

[0069] The second step is to pour the bacterial agents of fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast, and lactic acid bacteria into the culture medium at a mass ratio of 1:2 at a temperature of 20 to 45 degrees Celsius for reproduction, and stir twice a day. After 5 days, pour the cultured bacterial liquid into the newly prepared culture medium at a mass ratio of 1:3, and repeat this 5 times. After 38 days, one kilogram of bacterial agent can be made into about two tons of bacterial liquid.

[0070] The third step is to mix the expanded fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria in a ratio of 2:1:2:1:2:1 to obtain a probiotic solution, and spray it evenly on the pig manure at a mass ratio of 1:5. Turn the pig manure over every 3 hours to use the advantages of the probiotics to eliminate mold, anaerobic bacteria and other harmful bacteria in the pig manure. Generally, the odor of pig manure can be eliminated after 24 hours.

[0071] The fourth step is to control the temperature between 20 and 45 degrees Celsius, turn the pig manure once a day, and allow the probiotics to coexist with the odor-free pig manure to obtain bacterial manure.

[0072] Step 5: After 5 days, at the same temperature, add the pig manure to the bacterial manure at a volume ratio of 2:1 and stir evenly, turning it over every 3 hours for 8 times, and then once a day for 7 consecutive days. Repeat the above operation 2 more times. After 28 days, 1 catties of probiotic solution can treat about 700 catties of pig manure.

[0073] Step 6: Detection: When the ammonia nitrogen content is greater than 5%, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulose decomposing bacteria, and lactic acid bacteria are sprayed with bacterial solution and stirred at a mass ratio of 2:2:3:3:1:1, and the mass ratio of bacterial feces to bacterial solution is 280:1, once a day for 5 consecutive days. By adjusting the ratio of various probiotics in the bacterial community, the content of nitrite and aminopeptide nitrogen in pig manure can be effectively controlled.

[0074] The seventh step is to spray and stir the manure with photosynthetic cells, lactic acid bacteria, yeast, and fiber-decomposing bacteria in a ratio of 3:2:2:2.5 and a mass ratio of manure to bacteria of 530:1, once every 5 days.

[0075] Step 8. When the humidity of the pig manure reaches 10%, crush it or make it into granules to become organic probiotic fertilizer for tea trees.

[0076] Example 3

[0077] The first step is to prepare the culture medium according to the mass ratio of 100 catties of water, 10 catties of sugar, 5 catties of flour, 0.5 catties of salt, 0.5 catties of urea, and 0.5 catties of potassium dihydrogen phosphate.

[0078] The second step is to pour the bacterial agents of fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast, and lactic acid bacteria into the culture medium at a mass ratio of 1:5 at a temperature of 20 to 45 degrees Celsius, and stir twice a day. After 10 days, continue to pour the cultured bacterial liquid into the newly prepared culture medium at a mass ratio of 1:3, and repeat this 5 times. After 42 days, one kilogram of bacterial agent can be made into about two tons of bacterial liquid.

[0079] The third step is to mix the expanded fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria in a ratio of 1:2:1:2:1:2 to obtain a probiotic solution, and spray it evenly on the pig manure at a mass ratio of 1:15. Turn the pig manure over every 3 hours to use the advantages of the probiotics to eliminate mold, anaerobic bacteria and other harmful bacteria in the pig manure. Generally, the odor of pig manure can be eliminated after 24 hours.

[0080] The fourth step is to control the temperature between 20 and 45 degrees Celsius, turn the pig manure once a day, and allow the probiotics to coexist with the odor-free pig manure to obtain bacterial manure.

[0081] Step 5: After 10 days, at the same temperature, add the pig manure to the bacterial manure at a volume ratio of 5:1 and stir evenly, turning it every 3 hours for 8 times, and then once a day for 7 consecutive days. After 32 days, 1 catties of probiotic solution can treat about 700 catties of pig manure.

[0082] The sixth step is testing. When the nitrite content in each cubic decimeter of manure is greater than 0.2 mg, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulose decomposing bacteria, and lactic acid bacteria are sprayed with bacterial solution and stirred at a mass ratio of 3:3:2:2:1.5:1.0, and the mass ratio of manure to bacterial solution is 320:1, once a day for 12 consecutive days. By adjusting the ratio of various probiotics in the bacterial community, the content of nitrite and aminopeptide nitrogen in pig manure can be effectively controlled.

[0083] The seventh step is to spray and stir the manure with photosynthetic cells, lactic acid bacteria, yeast, and fiber-decomposing bacteria in a ratio of 2.5:1:1:3 and a mass ratio of manure to bacteria of 480:1, once every 5 days.

[0084] Step 8. When the humidity of the pig manure reaches 10%, crush it or make it into granules to become organic probiotic fertilizer for tea trees.

[0085] Comparative Example 1

[0086] The technical scheme of Comparative Example 1 is similar to that of Example 1, except that no fecal streptococci and photosynthetic bacteria are added.

[0087] Comparative Example 2

[0088] The technical scheme of Comparative Example 2 is similar to that of Example 1, except that yeast and Bacillus are not added.

[0089] Comparative Example 3

[0090] The technical solution of Comparative Example 3 is similar to that of Example 1, except that no cellulose decomposing bacteria or lactic acid bacteria are added.

[0091] Comparative Example 4

[0092] The technical scheme of Comparative Example 4 is similar to that of Example 1, except that fecal streptococci, photosynthetic bacteria, yeast, Bacillus, cellulose decomposing bacteria, and lactic acid bacteria are replaced by Bacillus, lactic acid bacteria, and actinomycetes.

[0093] Comparative Example 5

[0094] Mix pig manure, straw, rice husk and sawdust in a mass ratio of 4:2:1:1, and evenly spray the probiotic solution in a mass ratio of 1:10. The fermentation temperature is 55°C, and the pile is turned every 2 to 3 days for a total of 30 days.

[0095] Detection Methods

[0096] 1. Odor intensity evaluation (olfactory scoring method)

[0097] The odor of the bacterial feces after the fourth step was evaluated. An 8-member evaluation team was formed to score on a scale of 0 to 5 and take the average value. The evaluation criteria were: 0 points: no odor; 1 to 2 points: slight odor; 3 to 4 points: obvious odor; 5 points: extremely strong odor.

[0098] 2. Yield determination

[0099] The test site is located in Dayang Mountain, Jiexi, and the organic bacterial fertilizer of Example 1 of the present invention and the traditional organic fertilizer of the ordinary fermentation process of Comparative Example 5 are used respectively, and the test period is 7 months. After picking mature tea leaves in each test area, the weight of fresh leaves per mu (or per hectare) is calculated, the total weight of the fresh leaves is measured after drying, and the total weight of fresh leaves and dry leaf conversion rate of different treatment groups are determined.

[0100] 3. Nitrite and ammonia nitrogen content

[0101] (1) Detection of nitrite content

[0102] Sample extraction: The fertilizer sample was diluted with distilled water and filtered.

[0103] Color reaction: Add sulfonamide reagent and naphthylethylenediamine hydrochloride in sequence and let react for 15 minutes.

[0104] Detection: The absorbance was measured at 540 nm using a UV-Vis spectrophotometer.

[0105] Calculation: Use standard sodium nitrite solution to draw a standard curve, substitute the sample absorbance into the standard curve, and calculate the nitrite concentration.

[0106] (2) Detection of ammonia nitrogen content

[0107] Sample treatment: Use distilled water to extract the ammonia nitrogen in the fertilizer and filter it with filter paper.

[0108] Add reagents: Add potassium sodium tartrate to eliminate interfering ions, then add Nessler's reagent.

[0109] Detection: The absorbance was measured at a wavelength of 420 nm using a UV-Vis spectrophotometer.

[0110] Calculation: Use ammonium chloride solution of known concentration to draw a standard curve, and substitute the sample absorbance into the standard curve to calculate the ammonia nitrogen content.

[0111] 4. Sensory evaluation of tea

[0112] An 8-member review team was formed to use a 0-5 point scale to conduct sensory evaluation based on the appearance, aroma, taste and soup color of the tea. The evaluation criteria are as follows:

[0113] (1) Appearance standards

[0114] 5 points: The leaves of the tea tree are thick, green and smooth, with even stripes and rich luster.

[0115] 3 points: The tea leaf surface is slightly rough and the strips are slightly broken.

[0116] 1 point: The tea leaves are dull and the strands are loose.

[0117] (2) Soup color standard

[0118] 5 points: The color is golden and translucent, and the soup is like amber.

[0119] 3 points: The soup is yellowish in color, but slightly less transparent.

[0120] 1 point: The soup is dark or brown with sediment.

[0121] (3) Aroma standards

[0122] 5 points: The aroma is rich and fresh, lasting and layered.

[0123] 3 points: The aroma is medium, with a certain plant fragrance, but it is not long-lasting enough.

[0124] 1 point: The aroma is weak or has a strange smell.

[0125] (4) Taste Standard

[0126] 5 points: Rich and smooth flavor, sweet on the palate with a noticeable aftertaste.

[0127] 3 points: The taste is relatively light, with a sweet aftertaste but not lasting.

[0128] 1 point: The taste is bland or bitter.

[0129] Test results

[0130] 1. Odor evaluation

[0131] As can be seen from Table 1, in Examples 1 to 3, multiple strains work together to establish a stable micro-ecological system, effectively inhibit the reproduction of mold and anaerobic bacteria, and basically eliminate the odor of pig manure within 24 hours; multiple stirring ensures oxygen supply, reduces the formation of anaerobic environment, establishes the micro-ecological balance of probiotics, and completely converts pig manure into high-quality organic fertilizer. In contrast, Comparative Examples 1 to 4 reduce or replace the strain type, resulting in insufficient inhibition of mold and anaerobic bacteria or odor generation in the anaerobic environment, slow odor elimination, and poor adaptability of the bacterial community. In Comparative Example 5, a common fermentation method is used, but the frequency of turning the pile is low, the odor remains during the fermentation process, and harmful substances accumulate in the pig manure.

[0132] Table 1 Odor intensity evaluation

[0133] Rating average Example 1 0.3 Example 2 0.5 Example 3 0.6 Comparative Example 1 4.1 Comparative Example 2 3.2 Comparative Example 3 5.0 Comparative Example 4 3.8 Comparative Example 5 4.2

[0134] 2. Yield detection

[0135] In the organic fertilizer of Example 1, probiotics improve the root environment, allowing the tea tree to absorb more nutrients, increasing the growth rate and tea quality. It provides better water retention and aeration, improves soil structure, and increases biomass accumulation. The probiotics inhibit the growth of pathogens and improve the resistance of tea trees. Therefore, according to Table 2, compared with Comparative Example 5, Example 1 increases the dry tea yield by 51% and significantly increases the fresh leaf yield. Comparative Example 5 has a low frequency of turning the compost, and ammonia and nitrite remain in the fertilizer, resulting in poor root development and affecting the overall yield of the tea tree.

[0136] Table 2 Yield test results

[0137] Fresh leaf yield (kg / mu) Dry tea conversion rate (%) Dry tea yield (kg / mu) Example 1 250 25 62.5 Comparative Example 5 180 23 41.4

[0138] 3. Nitrite and ammonia nitrogen content

[0139] According to Table 3, Examples 1 to 3 are optimal for fermentation, with scientific strain ratios, and control of ammonia nitrogen volatilization and nitrite accumulation. Comparative Example 1 lacks fecal streptococci and photosynthetic bacteria, mold growth leads to nitrite accumulation, ammonia nitrogen is not completely absorbed, and the anaerobic environment exacerbates the stench. Comparative Example 2 lacks yeast and bacillus, resulting in nitrogen elements not being absorbed and fixed by the flora, ammonia volatilizes in large quantities, and the nitrite content is slightly increased, but the fermentation effect is poor. Comparative Example 3 lacks cellulose decomposing bacteria and lactic acid bacteria, the flora is unbalanced, and mold growth increases nitrite accumulation; but the ammonia nitrogen content is slightly increased by the action of other strains. Comparative Example 4 replaces the probiotic bacterial strain type, the strain has poor adaptability, and fails to effectively degrade nitrite and ammonia nitrogen, resulting in unsatisfactory fermentation effect. Comparative Example 5 is not completely fermented, and the anaerobic environment leads to the accumulation of nitrite and ammonia nitrogen. Although the fermentation temperature is high, the flora fails to establish a balance.

[0140] Table 3 Nitrite and ammonia nitrogen content

[0141] <![CDATA[Nitrite content (mg / dm 3 )]]> Ammonia nitrogen content (%) Example 1 0.17 4.2 Example 2 0.15 4.5 Example 3 0.22 4.8 Comparative Example 1 0.67 7.2 Comparative Example 2 0.23 6.5 Comparative Example 3 0.54 5.2 Comparative Example 4 0.86 6.8 Comparative Example 5 0.91 7.5

[0142] 4. Sensory evaluation of tea

[0143] Table 4 is the sensory evaluation results. The tea leaves of Examples 1 to 3 are thick, green and smooth. After being fried into Gongfu tea, the tea soup is golden and transparent, with a fragrant and wild flavor, and a softer and sweeter taste. Fecal streptococci and photosynthetic bacteria can inhibit the reproduction of molds and anaerobic bacteria, and maintain the health of the rhizosphere environment. The probiotics work synergistically, and lactic acid bacteria, Bacillus and yeasts jointly improve soil structure and enhance nutrient absorption. The organic fertilizer in the example promotes the synthesis of amino acids and polyphenols in tea trees, making the tea leaves have a strong aroma, smooth taste and lasting sweetness. During the metabolism of photosynthetic bacteria, substances that promote plant aroma are released, which increases the production of aromatic substances in tea. Cellulolytic bacteria decompose organic matter, improve soil aeration, and prevent compaction. Yeast and Bacillus absorb excess nitrogen, reducing the residual harmful substances in the soil.

[0144] Comparative Example 1 lacks the inhibitory effect of fecal streptococci and photosynthetic bacteria, resulting in a large number of molds multiplying during the fermentation of organic fertilizer. After application, the rhizosphere environment of the tea tree is affected, making the root system of the tea tree unable to effectively absorb nutrients, resulting in poor leaf growth, weak and non-persistent tea aroma, and rough leaf appearance. Comparative Example 2 lacks the ammonia absorption effect of yeast, ammonia volatilization increases, and the nitrogen absorption of the tea tree is unbalanced; the lack of Bacillus causes the temperature to rise slowly in the early stage of fermentation, pathogens are not effectively inhibited, and the soil environment deteriorates, ultimately resulting in the tea taste not smooth, astringent taste, and lack of sweetness. Comparative Example 3 lacks cellulose decomposing bacteria, and the organic matter in the soil is not fully decomposed, resulting in soil structure degradation and compaction; lack of lactic acid bacteria, the soil pH cannot be maintained in an appropriate range, affecting the nutrient absorption and healthy growth of the tea tree, and the final tea soup color is opaque, the taste is bitter, and lacks a sense of hierarchy. Comparative Example 4: Although actinomycetes have disease resistance, they are not suitable for rapid fermentation and oxygen-rich environments. Fermentation is not thorough, harmful gases accumulate during fermentation, the tea aroma is single and accompanied by odor, and the leaves are dull in appearance. Comparative Example 5: Fermentation by traditional methods, insufficient turning of the pile leads to insufficient oxygen supply, anaerobic bacteria multiply, odor increases, and fermentation is not thorough, resulting in dark tea soup color, sediment, and disappearance of aroma and aftertaste.

[0145] Table 4 Sensory evaluation of tea

[0146] Appearance Rating Soup color rating Aroma Rating Taste Rating Overall average score Example 1 5.0 5.0 5.0 5.0 5.0 Example 2 4.8 4.8 4.7 4.8 4.8 Example 3 4.7 4.5 4.6 4.7 4.6 Comparative Example 1 3.1 3.3 3.0 3.3 3.3 Comparative Example 2 3.8 3.4 3.5 3.2 3.5 Comparative Example 3 3.0 2.8 3.2 3.0 3.0 Comparative Example 4 2.5 2.8 2.7 2.9 2.7 Comparative Example 5 2.5 2.2 2.3 2.4 2.4

[0147] The organic bacterial fertilizer of the present invention improves the growth environment of soil and tea trees through a scientific multi-strain ratio, and significantly improves the appearance, aroma, soup color and taste of tea leaves. However, due to the lack of bacterial strains, insufficient fermentation or anaerobic environment, the nutritional absorption of tea trees is blocked, and the quality of tea leaves is greatly reduced. It can be seen that the correct use of organic bacterial fertilizer can effectively improve the sensory quality of tea leaves and promote the development of sustainable agriculture.

[0148] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. An organic fertilizer for tea trees, characterized in that: It is prepared from pig manure, fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast and lactic acid bacteria; the nitrite content in the prepared organic bacterial fertilizer is not more than 0.2mg / dm 3 , ammonia nitrogen content is not more than 5%.

2. A method for preparing the tea tree organic fertilizer according to claim 1, characterized in that: The following steps are involved: S1: Mixing the bacterial liquids of fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast and lactic acid bacteria to obtain a probiotic bacterial solution; S2: inoculate the probiotic bacteria solution on the pig manure and stir evenly to obtain bacterial manure; S3: Inoculate the bacterial liquid after reproduction of photosynthetic cells, lactic acid bacteria, yeast and fiber-decomposing bacteria into the bacterial feces, stir evenly, and obtain organic bacterial fertilizer for tea trees.

3. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: The bacterial solution after propagation in S1 is prepared by the following steps: S01: Inoculate fecal streptococci, photosynthetic bacteria, Bacillus, cellulolytic bacteria, yeast, and lactic acid bacteria into the culture medium respectively; S02: After 5 to 10 days of culture, take the culture medium and inoculate it into the newly prepared culture medium; S03: Repeat S02 until the total culture time reaches 38 to 42 days to obtain the propagated bacterial liquid.

4. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: In S01, fecal streptococci, photosynthetic bacteria, bacillus, cellulose decomposing bacteria, yeast, and lactic acid bacteria are inoculated into a culture medium at a mass ratio of 1:2-5; the culture medium is obtained by mixing water, sugar, flour, salt, urea, and potassium dihydrogen phosphate.

5. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: In S1, bacterial liquids after reproduction of fecal streptococci, photosynthetic bacteria, bacillus, cellulolytic bacteria, yeast and lactic acid bacteria are mixed in a mass ratio of 1-2:1-2:1-2:1-2:1-2:1-2:1-2 to obtain a probiotic bacterial group solution.

6. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: The mass ratio of the probiotic solution to pig manure in S2 is 1:5-15.

7. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: The S2 further comprises the following steps: inoculating the probiotic bacteria solution on the pig manure and stirring evenly to obtain bacterial manure after 5 to 10 days; adding the pig manure to the bacterial manure at a volume ratio of 2 to 5:1, stirring evenly for 28 to 32 days, and the pig manure becomes bacterial manure.

8. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: The nitrite and aminopeptide nitrogen content of the bacterial feces obtained in S2 was tested. The nitrite content in the bacterial feces should not exceed 0.2 mg / dm 3 , the ammonia nitrogen content must not exceed 5%.

9. The method for preparing tea tree organic fertilizer according to claim 8, characterized in that: Nitrite content in bacterial feces is greater than 0.2 mg / dm 3 When the ammonia nitrogen content is greater than 5%, fecal streptococci, photosynthetic bacteria, yeast, bacillus, cellulose-decomposing bacteria, and lactic acid bacteria are inoculated in a ratio of 2-3:2-3:2-3:2-3:1-1.5:1-1.5, according to the mass ratio of bacterial feces to probiotic bacteria solution of 280-320:1, and stirred evenly for 5-12 days.

10. The method for preparing tea tree organic fertilizer according to claim 2, characterized in that: In S3, photosynthetic cells, lactic acid bacteria, yeast, and fiber-decomposing bacteria are mixed in a mass ratio of 2.5-3:1-2:1-2:2.5-3, and after mixing, they are inoculated into the bacterial fertilizer in a mass ratio of 1:480-530.