Agricultural functional active protein preparation rich in strontium, calcium and zinc and preparation method thereof

Abstract

The invention belongs to the field of agriculture, and particularly relates to an agricultural functional active protein preparation rich in strontium, calcium and zinc and a preparation method thereof. The preparation method comprises the following steps: mixing traditional Chinese medicine raw materials, extracting, preparing a traditional Chinese medicine activation culture solution, and finally fermenting. According to the agricultural functional active protein preparation rich in strontium, calcium and zinc disclosed by the invention, trace elements such as strontium, calcium and zinc are all converted into organic states from chemical states after being biologically converted, so that the preparation has no toxic effect on soil and environment; after being biologically converted into an organic state, the leaf spray has high absorption efficiency on agricultural products, and the leaf spray absorption efficiency can reach 90% or above; leaf surface spraying can be carried out, use is convenient, the labor cost is low when the fertilizer is applied to each mu of land, and simultaneous supplement of multiple trace elements such as strontium, calcium and zinc is achieved; various pesticide residues can be rapidly and effectively degraded, and soil can be restored; the crop yield is increased; and the target organ quality is improved.

Description

Technical Field

[0001] The invention belongs to the field of agriculture, and specifically relates to a strontium, calcium and zinc-rich agricultural functional active protein preparation and a preparation method thereof. Background Art

[0002] At present, chemical preparations such as strontium chloride are mostly used to supplement crops with strontium, but they have the following disadvantages:

[0003] (1) Plants have a low absorption rate for strontium in chemical form.

[0004] (2) The strontium content in the product is relatively low, and the amount absorbed and converted by plants is even lower, which has a poor effect on increasing the strontium content in agricultural products.

[0005] (3) It is not possible to simultaneously supplement multiple trace elements such as strontium, calcium, and zinc.

[0006] (4) It fails to take into account the degradation of multiple pesticide residues and the restoration of soil.

[0007] (5) The effects of increasing crop yield and improving the quality of target organs are not significant.

[0008] Therefore, based on this, the technical solution of the present invention is proposed. Summary of the invention

[0009] In order to solve the problems existing in the prior art, the present invention provides a method for preparing a strontium, calcium and zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0010] (1) mixing liquorice, lily and black tea, soaking the mixture in water, and then heating and extracting the mixture to obtain an extract;

[0011] (2) heating the extract and then sequentially adding peptone, yeast extract, white sugar, and sodium chloride, and mixing them evenly to obtain a traditional Chinese medicine activation culture solution;

[0012] (3) adding red wine koji (purchased from Shiyan Gufeng Xinyu Food Co., Ltd.) and calcium chloride to the Chinese medicine activation culture solution, and fermenting under aerobic conditions to obtain a first fermentation solution;

[0013] (4) adding photosynthetic bacteria (purchased from Xiamen Mingde Weiye Biotechnology Co., Ltd.), yeast (purchased from Beijing Kezhan Biotechnology Co., Ltd.), and actinomycetes (purchased from Beijing Kezhan Biotechnology Co., Ltd.) to the first fermentation liquid in sequence, and continuing fermentation under aerobic conditions to obtain a second fermentation liquid;

[0014] (5) adding strontium chloride and zinc sulfate heptahydrate to the second fermentation broth in sequence, and continuing the fermentation under aerobic conditions, to obtain the strontium, calcium and zinc-rich agricultural functional active protein preparation.

[0015] Preferably, in step (1), the heating and leaching are performed three times; wherein:

[0016] The first extraction was carried out under boiling conditions for 4 hours;

[0017] The second extraction was carried out under boiling conditions for 2 hours;

[0018] The third extraction was carried out under boiling conditions for 1 h.

[0019] Preferably, in step (1), the mass ratio of licorice, lily and black tea is 35-40:40-45:35-40.

[0020] Preferably, in step (2), the mass ratio of peptone, yeast extract, white sugar and sodium chloride is 65-70:25-30:45-50:8-10.

[0021] Preferably, in step (3), the mass ratio of the red wine yeast to calcium chloride is 8-10:25-30.

[0022] Preferably, in step (4), the mass ratio of the photosynthetic bacteria, yeast, and actinomycetes is 3-5:8-10:8-10.

[0023] Preferably, in step (5), the mass ratio of strontium chloride to zinc sulfate heptahydrate is 45-50:45-50.

[0024] Based on the same technical concept, another embodiment of the present invention is to provide a strontium, calcium and zinc-rich agricultural functional active protein preparation obtained by the above preparation method.

[0025] The beneficial effects of the present invention are:

[0026] (1) The strontium, calcium, zinc and other trace elements in the agricultural functional active protein preparation rich in strontium, calcium and zinc obtained by the preparation method of the present invention are all converted from chemical state to organic state through biological transformation, and have no toxic effect on soil and environment.

[0027] (2) After the strontium, calcium and zinc-rich agricultural functional active protein preparation is bioconverted into an organic state, it has a high absorption efficiency when used on agricultural products, and the leaf spray absorption efficiency can reach more than 90%.

[0028] (3) The strontium, calcium and zinc-rich agricultural functional active protein preparation can be sprayed on leaves, is easy to use, and has low labor costs per acre of land.

[0029] (4) It can simultaneously supplement multiple trace elements such as strontium, calcium, and zinc.

[0030] (5) Take into account the degradation of multiple pesticide residues and the restoration of soil.

[0031] (6) Increase crop yield and improve target organ quality. DETAILED DESCRIPTION

[0032] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0033] Example 1

[0034] This embodiment provides a method for preparing a strontium, calcium and zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0035] (1) 40 kg of licorice, 45 kg of lily, and 40 kg of black tea are first soaked in water for 48 hours. The first time, cold water is boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice is poured out; the second time, boiling water is added, and simmered over low heat for 2 hours, and 350 kg of juice is poured out; the third time, boiling water is added, and simmered over low heat for 1 hour, and 300 kg of juice is poured out. The three times are combined to obtain 1000 kg of extract;

[0036] (2) boiling the 1000 kg extract, then adding 70 kg peptone, 30 kg yeast extract, 50 kg white sugar, and 10 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0037] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 10 kg of red wine yeast and 30 kg of calcium chloride, and fermenting under aerobic conditions at 38° C. for 10 days to obtain a first fermentation solution;

[0038] (4) at 12:00 noon on the 10th day of fermentation, 5 kg of photosynthetic bacteria were added, at 12:00 noon on the 13th day of fermentation, 10 kg of yeast were added, and at 12:00 noon on the 16th day of fermentation, 10 kg of actinomycetes were added; starting from the 10th day, the fermentation temperature was reduced to 32° C., and aeration fermentation was continued to obtain a second fermentation liquid;

[0039] (5) After 18 days of fermentation, 50 kg of strontium chloride and 50 kg of zinc sulfate heptahydrate were added to the second fermentation liquid. Starting from the 30th day, the fermentation temperature was reduced to 28° C. and the fermentation was continued with oxygen until the 49th day, thereby obtaining the strontium-calcium-zinc-rich agricultural functional active protein preparation.

[0040] Example 2

[0041] This embodiment provides a method for preparing a strontium, calcium and zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0042] (1) 35 kg of licorice, 40 kg of lily, and 35 kg of black tea are first soaked in water for 48 hours. The first time, cold water is boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice is poured out; the second time, boiling water is added, and simmered over low heat for 2 hours, and 350 kg of juice is poured out; the third time, boiling water is added, and simmered over low heat for 1 hour, and 300 kg of juice is poured out. The three times are combined to obtain 1000 kg of extract;

[0043] (2) boiling the 1000 kg extract, then adding 65 kg peptone, 25 kg yeast extract, 45 kg white sugar, and 8 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0044] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 8 kg of red wine yeast and 25 kg of calcium chloride, and fermenting under aerobic conditions at 38° C. for 10 days to obtain a first fermentation solution;

[0045] (4) at 12:00 noon on the 10th day of fermentation, 3 kg of photosynthetic bacteria were added, at 12:00 noon on the 13th day of fermentation, 8 kg of yeast were added, and at 12:00 noon on the 16th day of fermentation, 8 kg of actinomycetes were added; starting from the 10th day, the fermentation temperature was reduced to 32° C., and aeration fermentation was continued to obtain a second fermentation liquid;

[0046] (5) After 18 days of fermentation, 45 kg of strontium chloride and 45 kg of zinc sulfate heptahydrate were added to the second fermentation liquid. Starting from the 30th day, the fermentation temperature was reduced to 28° C. and the fermentation was continued with oxygen until the 49th day, thereby obtaining the strontium-calcium-zinc-rich agricultural functional active protein preparation.

[0047] Example 3

[0048] This embodiment provides a method for preparing a strontium, calcium and zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0049] (1) 38 kg of licorice, 42 kg of lily, and 38 kg of black tea are first soaked in water for 48 hours. The first time, cold water is boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice is poured out; the second time, boiling water is added, and simmered over low heat for 2 hours, and 350 kg of juice is poured out; the third time, boiling water is added, and simmered over low heat for 1 hour, and 300 kg of juice is poured out. The three times are combined to obtain 1000 kg of extract;

[0050] (2) boiling the 1000 kg extract, then adding 68 kg peptone, 28 kg yeast extract, 48 kg white sugar, and 9 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0051] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 9 kg of red wine yeast and 28 kg of calcium chloride, and fermenting under aerobic conditions at 38° C. for 10 days to obtain a first fermentation solution;

[0052] (4) at 12:00 noon on the 10th day of fermentation, 4 kg of photosynthetic bacteria were added, at 12:00 noon on the 13th day of fermentation, 9 kg of yeast were added, and at 12:00 noon on the 16th day of fermentation, 9 kg of actinomycetes were added; starting from the 10th day, the fermentation temperature was reduced to 32° C., and aeration fermentation was continued to obtain a second fermentation liquid;

[0053] (5) After 18 days of fermentation, 48 kg of strontium chloride and 48 kg of zinc sulfate heptahydrate were added to the second fermentation liquid. Starting from the 30th day, the fermentation temperature was reduced to 28° C. and the fermentation was continued with oxygen until the 49th day, thereby obtaining the strontium-calcium-zinc-rich agricultural functional active protein preparation.

[0054] Verification Example

[0055] The strontium calcium zinc agricultural functional active protein preparation of the present invention is usually applied by 100 times foliar spraying, and root irrigation can be used for some root crops. Generally, spraying 2-3 times during the formation of the target organ can achieve the effect.

[0056] Organic strontium, organic calcium and organic zinc were detected according to the existing method. The results are shown in Table 1.

[0057] Table 1

[0058] time Organic strontium / % Organic calcium / % Organic zinc / % 0d 0 0 0 7d 0 32 0 14d 0 66 0 21d 10 77 16 28d 35 86 42 35d 56 92 73 42d 82 96 89 49d 100 100 100

[0059] As shown in Table 1, strontium, calcium and zinc were all biotransformed by the fermentation process to achieve 100% organic transformation, and the final organic strontium content was about 5‰, the organic calcium content was about 9‰, and the organic zinc content was about 4‰.

[0060] Example 1 of the present invention (this product) and relevant commercial products (comparative products A, B, and C) were used as test objects, and tomatoes, peaches, and corns were sprayed, and their effects were tested.

[0061] The spraying effect on tomatoes is shown in Table 2.

[0062] Table 2

[0063]

[0064] The spraying method of this product is: spray after 100 times dilution; the comparison product A is: strontium chloride diluted 300 times and spray; the comparison product B is: calcium chloride diluted 300 times and spray; the comparison product C is: zinc sulfate heptahydrate diluted 300 times and spray; the same below.

[0065] The spraying effect on peach is shown in Table 3.

[0066] Table 3

[0067]

[0068] The spraying effect on corn is shown in Table 4.

[0069] Table 4

[0070]

[0071] It can be seen from Tables 2 to 4 that the effects of the products of the present invention are significantly better than similar products on the market.

[0072] The temperature in Shandong Province reached a maximum of 32 degrees and a minimum of 20 degrees at the end of May. The main pesticide mixture (1000 times of phoxim (45% phoxim emulsifiable concentrate), 1000 times of phorate (55% phorate), 200 times of triazophos (4% triazophos), 500 times of thiamethoxam (25% thiamethoxam), 500 times of imidacloprid (20% concentrated soluble powder)) was irrigated to the roots of tomatoes, peaches and corns for pesticide residue detection. 1000 jin / mu, half a month after irrigating the roots, 200 times of Example 1 (this product) of the present invention was irrigated 2 times every 7 days, and 300 times of Example 1 (this product) of the present invention was sprayed on the leaves every 2 days for 3 times. Soil and target organs (tomato fruits, peach fruits, corn cobs) were taken as detection objects every 2 days, and their pesticide residues and soil health indicators were detected. The results are shown in Table 5.

[0073] Table 5

[0074]

[0075] Table 5 shows that the pesticide residues in the soil of tomatoes, peaches and corns were basically completely degraded about half a month after the application of this product. Under the condition that the pesticide residues of phoxim, phorate, triazophos, thiamethoxam and imidacloprid in tomato fruits, peach fruits and corn kernels exceeded the national standard by 400 times, the pesticide residues were reduced to below the national limit on the 4th day after the use of this product, and the pesticide residues were reduced to 0 on the 10th day.

[0076] Table 6

[0077] Day 0 Day 2 Day 4 Day 6 Day 8 Day 10 Day 12 Day 14 Day 16 Tomato Soil Soil bulk density (g / cm3) 1.42 1.44 1.45 1.46 1.48 1.49 1.495 1.497 1.502 PH 6.1 6.58 6.91 6.95 7.05 7.06 7.07 7.07 7.08 Organic matter (g / kg) 12.4 12.48 12.56 12.81 13.42 13.52 13.67 13.86 13.91 Microbial biomass carbon (mg / kg) 124 178 192 201 234 245 251 260 265 Peach Soil Soil bulk density (g / cm3) 1.38 1.4 1.44 1.51 1.56 1.6 1.62 1.64 1.66 PH 8.2 8.1 7.9 7.7 7.5 7.3 7.2 7.1 7 Organic matter (g / kg) 13.1 13.4 13.45 13.48 14 14.3 14.8 15.3 15.4 Microbial biomass carbon (mg / kg) 105 145 162 170 204 218 221 218 224 Corn Soil Soil bulk density (g / cm3) 1.24 1.41 1.43 1.48 1.53 1.61 1.65 1.66 1.66 PH 8.1 7.8 7.6 7.5 7.2 7 6.8 6.7 6.6 Organic matter (g / kg) 10.41 11.87 12.12 12.42 12.43 13.34 13.42 13.42 13.44 Microbial biomass carbon (mg / kg) 118 135 142 152 176 183 191 205 206

[0078] It can be seen from Table 6 that the application of Example 1 of the present invention (this product) can increase soil bulk density, adjust pH, increase soil organic matter, and increase soil microbial biomass carbon, thereby repairing the soil.

[0079] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for preparing a strontium, calcium and zinc-rich agricultural functional active protein preparation, characterized in that: The preparation method comprises the following steps: (1) mixing liquorice, lily and black tea, soaking the mixture in water, and then heating and extracting the mixture to obtain an extract; (2) heating the extract and then sequentially adding peptone, yeast extract, white sugar, and sodium chloride, and mixing them evenly to obtain a traditional Chinese medicine activation culture solution; (3) adding red wine yeast and calcium chloride to the traditional Chinese medicine activation culture solution, and fermenting under aerobic conditions to obtain a first fermentation solution; (4) adding photosynthetic bacteria, yeast, and actinomycetes to the first fermentation liquid in sequence, and continuing fermentation under aerobic conditions to obtain a second fermentation liquid; (5) adding strontium chloride and zinc sulfate heptahydrate to the second fermentation broth in sequence, and continuing the fermentation under aerobic conditions, to obtain the strontium, calcium and zinc-rich agricultural functional active protein preparation.

2. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (1), the heating and leaching are performed three times; wherein: The first extraction was carried out under boiling conditions for 4 hours; The second extraction was carried out under boiling conditions for 2 hours; The third extraction was carried out under boiling conditions for 1 h.

3. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (1), the mass ratio of liquorice, lily and black tea is 35-40:40-45:35-40.

4. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (2), the mass ratio of peptone, yeast extract, white sugar and sodium chloride is 65-70:25-30:45-50:8-10.

5. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (3), the mass ratio of the red wine yeast to calcium chloride is 8-10:25-30.

6. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (4), the mass ratio of the photosynthetic bacteria, yeast and actinomycetes is 3-5:8-10:8-10.

7. The method for preparing the strontium, calcium and zinc-rich agricultural functional active protein preparation according to claim 1, characterized in that: In step (5), the mass ratio of strontium chloride to zinc sulfate heptahydrate is 45-50:45-50.

8. The agricultural functional active protein preparation rich in strontium, calcium and zinc obtained by the preparation method according to any one of claims 1 to 7.

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