An iridium complex for dynamic detection of mitochondrial viscosity and tumor differentiation, and a preparation method and use thereof

By designing the iridium complex Ir-visc and utilizing a double-lock mitochondrial targeting strategy and positive charge binding, the problem of existing probes' dependence on membrane potential was solved, achieving efficient mitochondrial viscosity monitoring and cancer diagnosis, and possessing deep tissue imaging capabilities.

CN119708075BActive Publication Date: 2025-10-14ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Application Number
CN202411840668.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-13
Publication Date
2025-10-14
Estimated Expiration
2044-12-13

AI Technical Summary

Technical Problem

Existing mitochondrial viscosity probes are highly dependent on mitochondrial membrane potential, have short luminescence lifetime and insufficient tissue penetration depth, which limits their application in cancer diagnosis and biological research.

Method used

An iridium complex, Ir-visc, was designed. Through a double-lock mitochondrial targeting strategy, C^N and N^N ligands modified with benzothiazole derivatives and electron-rich thiophene rings were used to combine positive charges with non-covalent bonds to mitochondrial membrane proteins, achieving high quantum yield of viscosity activation and deep tissue imaging.

Benefits of technology

It achieves stable monitoring of mitochondrial viscosity and tumor differentiation, has high quantum yield, long luminescence lifetime and deep tissue penetration ability, can effectively distinguish cancer cells from normal cells, and provides multi-level visualization tools.

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Abstract

The application relates to the field of biological medicine, and discloses an iridium complex for dynamic detection of mitochondrial viscosity and tumor differentiation, a preparation method and application thereof, and a structure formula of the iridium complex is as follows: The iridium complex Ir-visc in the application is based on a double-locking targeting strategy of electrostatic adsorption and probe-protein docking, does not depend on MMP, and stably anchors mitochondria in cells; the iridium complex exhibits excellent high-viscosity-activated one-photon (OP) and two-photon (TP) phosphorescence characteristics, is not disturbed by other factors in a living body, can be used for monitoring viscosity changes in a complex biological system; can be used as a mitochondrial phosphorescence probe to differentiate tumor cells and normal cells, and monitor changes of mitochondrial viscosity of cells under the stimulation of starvation and an antifungal drug nystatin; and not only has strong tumor tissue recognition capability, but also has deep tissue penetration and in-vivo imaging capability.
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