Device and method for extracting polyphenols from fermented fruits and vegetables
By designing a fruit and vegetable fermentation polyphenol extraction device that uses a reaction tank for fermentation and extraction, the problem of insufficient mixing of fruit and vegetable liquid and extract in the prior art and many bubbles is solved, and the short-term full fermentation of fruit and vegetable and the full mixing of extract liquid is achieved, and the efficiency of polyphenol extraction is improved.
Patent Information
- Application Number
- CN202510228339.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-16
- Estimated Expiration
- 2045-02-28
AI Technical Summary
The existing fruit and vegetable fermentation polyphenol extraction device requires two containers during the fermentation and extraction process, and the stirring method causes the fruit and vegetable liquid and the extract to be insufficiently mixed, and there are many bubbles, which affects the polyphenol extraction efficiency.
A fruit and vegetable fermentation polyphenol extraction device is designed, and fermentation and extraction are performed using a reaction tank. The fermentation and extraction control mechanism is used to achieve full mixing of the fruit and vegetable liquid and the extract liquid to reduce bubble generation.
The short-term full fermentation of fruits and vegetables and the full mixing of the extract liquid are achieved, which reduces the generation of bubbles when extracting polyphenols and improves the polyphenol extraction efficiency.
Smart Images

Figure CN119709375B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of polyphenol extraction from fruit and vegetable fermentation, and particularly relates to a device and method for extracting polyphenol from fruit and vegetable fermentation. Background Art
[0002] As people pursue a healthy life, polyphenols in fruits and vegetables have attracted much attention. Polyphenols are a class of compounds with antioxidant activity. They have strong antioxidant capacity, lower cholesterol, reduce arteriosclerosis, and slow down aging. They are widely used in food, medicine, and cosmetics. Fruit and vegetable polyphenols can be extracted by fermenting fruits and vegetables and then using a mixed extraction solution. According to the solubility differences of the extracted components in different solvents, appropriate solvents are selected to separate the active ingredients from the extraction raw materials.
[0003] In the prior art, when performing fruit and vegetable fermentation and polyphenol extraction, the fruit and vegetable fermentation polyphenol extraction device usually requires two containers for operation, and stirring is used to assist fermentation and extraction during fermentation and polyphenol extraction, which is not conducive to the full fermentation of fruits and vegetables in a short time, and cannot make the extract liquid fully mixed. Moreover, when stirring the fruit and vegetable liquid and the extract liquid, it is easy to have many bubbles, which is not conducive to the extraction of polyphenols.
[0004] Therefore, it is necessary to invent a fruit and vegetable fermentation polyphenol extraction device and method to solve the above problems, which can mix the fermentation of fruits and vegetables and polyphenol extraction in the same container, reduce the hidden danger of pollution caused by the transfer of fruit and vegetable liquid, and facilitate the fruits and vegetables to be fully fermented and the extract to be fully mixed in a short time, thereby reducing the generation of bubbles when extracting polyphenols. Summary of the invention
[0005] In view of the above problems, the present invention provides a device and method for extracting polyphenols from fermented fruits and vegetables to solve the problems raised in the above background technology.
[0006] To achieve the above-mentioned object, the present invention provides the following technical solutions: a device and method for extracting polyphenols from fermented fruits and vegetables, comprising a reaction tank, the reaction tank being used for extracting polyphenols from fermented fruits and vegetables, a fermentation extraction control mechanism being rotatably provided in the middle of the reaction tank, a fermentation extraction lifting control mechanism being fixedly provided on one side of the reaction tank, and a rotation control mechanism being fixedly provided at the bottom end of the fermentation extraction control mechanism, wherein:
[0007] The fermentation extraction control mechanism comprises a positioning shaft rotating on the inner wall of the reaction tank, positioning strips are fixedly provided on both sides of the outer wall of the positioning shaft, a positioning sleeve is provided on the outer wall of the positioning shaft through the positioning and sliding of the positioning strips, two stirring and crushing shafts are fixedly provided at one end of the positioning sleeve, a limiting groove is fixedly provided at the middle position of the outer wall of the positioning sleeve, a positioning seat is fixedly provided at the bottom end of the outer wall of the positioning sleeve, flow stabilizing frames are fixedly provided on both sides of the positioning seat, a filter frame is rotatably provided on the outer wall of the positioning shaft through the limiting groove, a protective pad is fixedly provided on the outer wall of the filter frame, and the filter frame contacts the inner wall of the reaction tank through the protective pad;
[0008] An air inlet pipe is inserted through one end of the bottom of the outer wall of the reaction tank, and an extract feed pipe is inserted through the other end of the bottom of the outer wall of the reaction tank. One end of the air inlet pipe and one end of the extract feed pipe are connected through a communicating vessel. A flow divider is fixedly provided on the top of the communicating vessel. The communicating vessel is rotatably provided with a connecting frame through the flow divider at the top. A plurality of connecting seats are provided on the top of the connecting frame, and an auxiliary sealing mechanism is fixedly provided on one end of each of the plurality of connecting seats.
[0009] The rotation control mechanism includes a first limit seat fixed at the bottom end of the positioning shaft and a second limit seat fixed at the middle position of the top of the connecting frame, a fixing sleeve is fixedly arranged at the top of the second limit seat, a fixing block is slidingly limited at the middle position of the fixing sleeve, a third limit seat is fixedly arranged at the top of the fixing block, a plurality of slope limit blocks are fixedly arranged at the bottom end of the first limit seat and the top end of the third limit seat, and the top end of the second limit seat and the bottom end of the third limit seat are connected by a reset spring.
[0010] Preferably, a first motor is fixedly provided at the middle position of the top of the reaction tank, and the output end of the first motor is fixedly connected to the top end of the positioning shaft.
[0011] Preferably, one end of the top of the reaction tank is connected to a feed port, one side of the outer wall of the reaction tank is connected to a slag discharge port, and the bottom end of the reaction tank is connected to a liquid discharge port.
[0012] Preferably, the feed port, slag discharge port and liquid discharge port are all provided with control valves.
[0013] Preferably, the fermentation and extraction lifting control mechanism includes a control frame fixed to one side of the reaction tank, a positioning screw is rotatably provided in the middle position of the control frame, one end of the outer wall of the positioning screw is threadedly provided with a lifting frame, one end of the outer wall of the lifting frame is slidably connected to one end of the top of the control frame, a connecting ring is fixedly provided at the top of the lifting frame, a lifting seat is fixedly provided on one side of the connecting ring, both ends of the lifting seat pass through the top of the reaction tank and are respectively fixedly connected to the two ends of the top of the filter frame, and a protective ring is fixedly provided in the middle position of the top of the lifting seat.
[0014] Preferably, a second motor is fixedly provided on the top end of the control frame, and an output end of the second motor is fixedly connected to the top end of the positioning screw rod.
[0015] Preferably, the height of the slag discharge port is flush with the highest height of the filter frame.
[0016] Preferably, the auxiliary sealing mechanism includes a positioning rod fixed at the top of the connecting frame, an adjustment seat is inserted into one end of the outer wall of the positioning rod, a protective cover is fixed to the top of the adjustment seat, a sealing gasket is fixed to the bottom end of the protective cover, a positioning spring is inserted into the other end of the outer wall of the positioning rod, and a positioning block is fixed to the top of the positioning rod.
[0017] Preferably, an intelligent control panel is fixedly provided on one side of the reaction tank, and the first motor and the second motor are electrically connected to an external power supply through the intelligent control panel.
[0018] A method for extracting polyphenols from fruit and vegetable fermentation, the method is as follows:
[0019] Step 1: Preliminary preparation: Wash, drain, and crush the fresh fruits and vegetables;
[0020] Step 2, aeration fermentation: pour the crushed fruit and vegetable pulp into the reaction tank 1, stir and aerate and ferment at a constant temperature of 30° C. for a certain period of time, lift the filter rack 602 through the fermentation extraction lifting control mechanism 7 to filter out the remaining waste residue after fermentation, and discharge the waste residue to obtain fermentation liquid;
[0021] Step 3, polyphenol extraction: 40% ethanol extract was mixed evenly with aeration, the solid-liquid ratio was 1g:25mL, the extraction time was 2h, the extraction temperature was 50°C, and the supernatant was centrifuged at 3000r / min for 30min. The supernatant was allowed to flow steadily to remove bubbles and then precipitate. After precipitation, the precipitate and the supernatant were discharged in sequence through the bottom discharge port 5. The precipitate was reset to the reaction tank 1, and the reaction tank 1 was extracted and centrifuged at 3000r / min for 30min. The extraction time was 2h and the extraction temperature was 50°C. The extraction was repeated once, the supernatant was allowed to flow steadily to remove bubbles and then precipitate. After precipitation, the precipitate and the supernatant were discharged in sequence through the bottom discharge port 5.
[0022] Step 4, polyphenol extract: combine the two supernatants to obtain a polyphenol extract;
[0023] Step 5, concentration, purification, and elution: the polyphenol extract was concentrated by rotary evaporator, purified by AB-8 macroporous resin, and eluted with 50% ethanol solution;
[0024] Step 6, powder making: then concentrate by rotary evaporator, vacuum freeze-dry to constant mass, and obtain fruit and vegetable polyphenol powder.
[0025] Technical effects and advantages of the present invention:
[0026] 1. The present invention drives the positioning shaft to rotate rapidly in the forward direction through the output end of the first motor fixed on the top of the reaction tank. Through the direction control of the rotation control mechanism, the positioning shaft drives the positioning sleeve, the stirring and crushing shaft, the positioning seat, the flow stabilizing frame and the connecting frame to rotate through the positioning of the positioning bar. The top of the connecting frame is connected to the connecting vessel through the diverter frame. With the rotation of the connecting frame, a rotating aeration effect is presented at the bottom of the fruit and vegetable liquid, so that the fruit and vegetable fermentation liquid and the air are fully mixed, so as to ensure that the microorganisms in the fruit and vegetable liquid can obtain enough oxygen to carry out normal metabolic activities and improve the fermentation effect of the fruit and vegetable liquid. The extract enters the inner wall of the reaction tank through the extract feed pipe in the same way. The extract and the gas are pumped in alternately, so that the extract and the fruit and vegetable liquid are fully mixed;
[0027] 2. The present invention drives the positioning shaft to rotate slowly in the reverse direction through the output end of the first motor fixed at the top of the reaction tank, so that the first limit seat fixed at the bottom end of the positioning shaft is squeezed on the slope through the slope limit block and the slope limit block fixed at the top of the third limit seat, and is squeezed by the return spring fixed at the top of the second limit seat and the bottom of the third limit seat. Through the compression of the fixed sleeve and the fixed block, the third limit seat drives the slope limit block to move downward, so that when the positioning shaft rotates slowly in the reverse direction, it can only drive the flow stabilizing frame fixed on the outer wall of the positioning seat to rotate slowly on the surface of the liquid, so that the liquid and bubbles on the surface slowly pass through the flow stabilizing frame, so that the liquid is stable and the bubbles are removed, so as to avoid the influence of the foam that cannot be dissipated on the extraction concentration, and improve the polyphenol extraction efficiency;
[0028] 3. The present invention facilitates aeration, extract feeding control and steady flow and bubble removal control by controlling the first motor in different directions. A single electrical appliance performs multi-functional control, thereby reducing the production cost of the fruit and vegetable fermentation polyphenol extraction device;
[0029] 4. The present invention slides along the top of the control frame by a lifting frame threadedly connected to the outer wall of the positioning screw rod, so that the lifting frame drives the lifting seat to be lifted through the connecting ring, so that the lifting seat drives the filter frame to be lifted through the top of the reaction tank, and the waste residue remaining in the fermentation inside the reaction tank is filtered. The waste residue is pushed by the fruit and vegetable liquid to gather at the residue discharge port and is discharged through the residue discharge port, so that the flow stabilizing frame is stably immersed in the liquid surface;
[0030] 5. The present invention uses gas or liquid to impact the inside of the connecting seat, so that the protective cover in contact with the top of the connecting seat drives the sealing gasket to move upward. When pumping gas and pumping liquid are finished, the positioning spring inserted in the outer wall of the positioning rod is elastically deformed, so that the protective cover and the sealing gasket are stably sealed on the top of the connecting seat, reducing the hidden danger of backflow of fruit and vegetable liquid.
[0031] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0033] Figure 1 It is a three-dimensional schematic diagram of the present invention;
[0034] Figure 2 This is a schematic diagram of the internal structure of the fruit and vegetable fermentation polyphenol extraction device of the present invention;
[0035] Figure 3 Schematic diagram of the internal structure of the device for extracting polyphenols from fermented fruits and vegetables according to the present invention;
[0036] Figure 4 It is a schematic diagram of the structure of the fermentation extraction lifting control mechanism of the present invention;
[0037] Figure 5 It is a schematic diagram of the cross-sectional structure of the fermentation extraction control mechanism of the present invention;
[0038] Figure 6 It is a schematic diagram of the structure of the fermentation extraction control mechanism of the present invention;
[0039] Figure 7 It is a partial structural schematic diagram of the fermentation extraction control mechanism of the present invention;
[0040] Figure 8 The present invention Figure 5 The enlarged structural diagram at A in the middle;
[0041] Fig. 9 It is a schematic diagram of the structure of the local positioning sleeve of the fermentation extraction control mechanism of the present invention;
[0042] Fig.10 It is a schematic diagram of the extrusion structure of the rotation control mechanism of the present invention;
[0043] Fig.11 It is a schematic diagram of the separate structure of the rotation control mechanism of the present invention;
[0044] Fig.12 The present invention Figure 7 Enlarged structural diagram at B in the middle.
[0045] In the figure: 1, reaction tank; 2, feed port; 3, slag discharge port; 4, first motor; 5, discharge port; 6, fermentation and extraction control mechanism; 601, positioning shaft; 602, filter frame; 603, protective pad; 604, positioning bar; 605, positioning sleeve; 606, limit groove; 607, air inlet pipe; 608, extracting liquid feed pipe; 609, connecting vessel; 610, connecting frame; 611, diverter frame; 612, connecting seat; 613, stirring and crushing shaft; 614, positioning seat; 615, steady flow frame; 7, fermentation and extraction lifting control mechanism; 701 , control frame; 702, positioning screw; 703, second motor; 704, lifting frame; 705, connecting ring; 706, lifting seat; 707, protective ring; 8, rotation control mechanism; 801, first limit seat; 802, third limit seat; 803, second limit seat; 804, fixing sleeve; 805, fixing block; 806, reset spring; 807, slope limit block; 9, auxiliary sealing mechanism; 901, positioning rod; 902, positioning spring; 903, positioning block; 904, adjusting seat; 905, protective cover; 906, sealing gasket. DETAILED DESCRIPTION
[0046] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0047] The present invention provides Figure 1-12 The device and method for extracting polyphenols from fermented fruits and vegetables shown in the figure include a reaction tank 1, which is used for extracting polyphenols from fermented fruits and vegetables. A fermentation extraction control mechanism 6 is rotatably provided in the middle of the reaction tank 1, a fermentation extraction lifting control mechanism 7 is fixedly provided on one side of the reaction tank 1, and a rotation control mechanism 8 is fixedly provided at the bottom end of the fermentation extraction control mechanism 6, wherein:
[0048] The fermentation and extraction control mechanism 6 includes a positioning shaft 601 rotating on the inner wall of the reaction tank 1, positioning bars 604 are fixedly provided on both sides of the outer wall of the positioning shaft 601, a positioning sleeve 605 is provided on the outer wall of the positioning shaft 601 through the positioning and sliding of the positioning bars 604, two stirring and crushing shafts 613 are fixedly provided at one end of the positioning sleeve 605, a limiting groove 606 is fixedly provided at the middle position of the outer wall of the positioning sleeve 605, a positioning seat 614 is fixedly provided at the bottom end of the outer wall of the positioning sleeve 605, and flow stabilizing frames 615 are fixedly provided on both sides of the positioning seat 614, a filter frame 602 is rotatably provided on the outer wall of the positioning shaft 601 through the limiting groove 606, a protective pad 603 is fixedly provided on the outer wall of the filter frame 602, and the filter frame 602 contacts the inner wall of the reaction tank 1 through the protective pad 603;
[0049] An air inlet pipe 607 is inserted through one end of the bottom of the outer wall of the reaction tank 1, and an extract feed pipe 608 is inserted through the other end of the bottom of the outer wall of the reaction tank 1. One end of the air inlet pipe 607 and one end of the extract feed pipe 608 are connected through a connecting vessel 609. A flow divider 611 is fixedly provided at the top of the connecting vessel 609. A connecting frame 610 is rotatably provided on the connecting vessel 609 through the top flow divider 611. A plurality of connecting seats 612 are provided at the top of the connecting frame 610, and one end of each of the plurality of connecting seats 612 is fixedly provided with an auxiliary sealing mechanism 9.
[0050] The rotation control mechanism 8 includes a first limit seat 801 fixed at the bottom end of the positioning shaft 601 and a second limit seat 803 fixed at the middle position of the top of the connecting frame 610, a fixing sleeve 804 is fixed at the top end of the second limit seat 803, a fixing block 805 is provided at the middle position of the fixing sleeve 804 for limiting sliding, a third limit seat 802 is fixed at the top end of the fixing block 805, a plurality of slope limit blocks 807 are fixed at the bottom end of the first limit seat 801 and the top end of the third limit seat 802, and the top end of the second limit seat 803 and the bottom end of the third limit seat 802 are connected via a return spring 806;
[0051] A first motor 4 is fixedly provided at the middle position of the top of the reaction tank 1, and the output end of the first motor 4 is fixedly connected to the top end of the positioning shaft 601;
[0052] When the fruit and vegetable fermentation polyphenol extraction device is needed, the crushed fruit and vegetable liquid is transported into the reaction tank 1 through the feed port 2, and the positioning shaft 601 is driven to rotate rapidly in the positive direction through the output end of the first motor 4 fixed at the top of the reaction tank 1, so that the positioning shaft 601 drives the positioning sleeve 605, the stirring and crushing shaft 613, the positioning seat 614 and the flow stabilizing frame 615 to rotate through the positioning of the positioning bar 604, and the first limiting seat 801 fixed at the bottom end of the positioning shaft 601 is squeezed at a right angle with the slope limiting block 807 fixed at the top end of the third limiting seat 802 through the slope limiting block 807, so that the third limiting seat 802 is fixed through the fixing sleeve 80 4 and the fixed block 805 drive the second limiting seat 803 and the connecting frame 610 to rotate. The bottom end of the connecting frame 610 is connected to the connecting vessel 609 through the diverter frame 611. When the external air pump pumps gas through the air inlet pipe 607, the gas is discharged through the connecting seat 612 fixedly provided on the top of the connecting frame 610. With the rotation of the connecting frame 610, a rotating aeration effect is present at the bottom of the fruit and vegetable liquid, so that the fruit and vegetable fermentation liquid is fully mixed with the air. With the help of the rotating stirring of the stirring and crushing shaft 613 and the stabilizing frame 615, it is ensured that the microorganisms in the fruit and vegetable liquid can obtain enough oxygen to carry out normal metabolic activities, thereby improving the fermentation effect of the fruit and vegetable liquid;
[0053] The 40% ethanol extract enters the inner wall of the reaction tank 1 through the extract feed pipe 608 in the same way, and the extract and the fruit and vegetable liquid are fully mixed by alternately pumping the extract and the gas;
[0054] The output end of the first motor 4 fixed at the top of the reaction tank 1 drives the positioning shaft 601 to rotate slowly in the reverse direction, so that the first limit seat 801 fixed at the bottom end of the positioning shaft 601 is squeezed on the slope by the slope limit block 807 and the slope limit block 807 fixed on the top of the third limit seat 802, and is squeezed by the return spring 806 fixed at the top of the second limit seat 803 and the bottom of the third limit seat 802. Through the compression of the fixing sleeve 804 and the fixing block 805, the third limit seat 802 drives the slope limit block 807 to move downward, so that when the positioning shaft 601 rotates slowly in the reverse direction, the connecting frame 610 cannot rotate, and can only drive the flow stabilizing frame 615 fixed on the outer wall of the positioning seat 614 to rotate slowly on the surface of the liquid, so that the liquid and bubbles on the surface slowly pass through the flow stabilizing frame 615, so that the liquid is stable and the bubbles are removed, so as to avoid the influence of the foam that cannot be dissipated on the extraction concentration, thereby improving the polyphenol extraction efficiency.
[0055] As a specific embodiment of the present invention, one end of the top of the reaction tank 1 is connected to a feed port 2, one side of the outer wall of the reaction tank 1 is connected to a slag discharge port 3, and the bottom end of the reaction tank 1 is connected to a liquid discharge port 5.
[0056] The feed port 2, the slag discharge port 3 and the liquid discharge port 5 are all provided with control valves.
[0057] As a specific embodiment of the present invention, the fermentation extraction lifting control mechanism 7 includes a control frame 701 fixed on one side of the reaction tank 1, a positioning screw 702 is rotatably provided at the middle position of the control frame 701, one end of the outer wall of the positioning screw 702 is threadedly provided with a lifting frame 704, one end of the outer wall of the lifting frame 704 is slidably connected with one end of the top of the control frame 701, a connecting ring 705 is fixedly provided at the top of the lifting frame 704, a lifting seat 706 is fixedly provided on one side of the connecting ring 705, both ends of the lifting seat 706 pass through the top of the reaction tank 1 and are respectively fixedly connected to the two ends of the top of the filter frame 602, and a protective ring 707 is fixedly provided at the middle position of the top of the lifting seat 706;
[0058] A second motor 703 is fixedly provided at the top of the control frame 701, and an output end of the second motor 703 is fixedly connected to the top of the positioning screw rod 702;
[0059] After the fermentation is finished, the output end of the second motor 703 fixed on the top of the control frame 701 drives the positioning screw 702 to rotate, so that the lifting frame 704 threadedly connected to the outer wall of the positioning screw 702 slides along the top of the control frame 701, so that the lifting frame 704 drives the lifting seat 706 to be lifted through the connecting ring 705, so that the lifting seat 706 passes through the top of the reaction tank 1 to drive the filter frame 602 to be lifted, and the filter frame 602 contacts the inner wall of the reaction tank 1 through the protective pad 603 fixed on the outer wall. Due to the limiting groove 606 on the outer wall of the positioning sleeve 605, when the filter frame 602 is lifted, the limiting groove 606 drives the positioning sleeve 605, the positioning seat 614, the flow stabilizing frame 615 and the stirring and crushing shaft 613 to be stably lifted, so as to filter the waste residue remaining after fermentation inside the reaction tank 1. Since the filter frame 602 has a certain inclination angle, when the filter frame 602 is lifted, the waste residue is pushed by the fruit and vegetable liquid, so that the waste residue is gathered at the residue discharge port 3 and discharged through the residue discharge port 3, so that the flow stabilizing frame 615 is stably immersed in the liquid surface.
[0060] As a specific implementation of the present invention, the height of the slag discharge port 3 is flush with the highest height of the filter frame 602 .
[0061] As a specific embodiment of the present invention, the auxiliary sealing mechanism 9 includes a positioning rod 901 fixed to the top of the connecting frame 610, an adjustment seat 904 is inserted at one end of the outer wall of the positioning rod 901, a protective cover 905 is fixed at the top of the adjusting seat 904, a sealing gasket 906 is fixed at the bottom of the protective cover 905, a positioning spring 902 is inserted at the other end of the outer wall of the positioning rod 901, and a positioning block 903 is fixed at the top of the positioning rod 901;
[0062] When gas or extract is pumped into the air inlet pipe 607 or the extract feed pipe 608, the gas or liquid impacts the inside of the connecting seat 612, so that the protective cover 905 in contact with the top of the connecting seat 612 drives the sealing gasket 906 to move upward. When the pumping of gas and liquid is finished, the elastic deformation of the positioning spring 902 inserted in the outer wall of the positioning rod 901 makes the protective cover 905 and the sealing gasket 906 stably sealed at the top of the connecting seat 612, thereby reducing the hidden danger of backflow of fruit and vegetable liquid.
[0063] As a specific embodiment of the present invention, an intelligent control panel is fixedly provided on one side of the reaction tank 1, and the first motor 4 and the second motor 703 are both electrically connected to an external power supply through the intelligent control panel.
[0064] As a specific embodiment of the present invention, a method for extracting polyphenols from fruit and vegetable fermentation is as follows:
[0065] Step 1: Preliminary preparation: Wash, drain, and crush the fresh fruits and vegetables;
[0066] Step 2, aeration fermentation: pour the crushed fruit and vegetable pulp into the reaction tank 1, stir and aerate and ferment at a constant temperature of 30° C. for a certain period of time, lift the filter rack 602 through the fermentation extraction lifting control mechanism 7 to filter out the remaining waste residue after fermentation, and discharge the waste residue to obtain fermentation liquid;
[0067] Step 3, polyphenol extraction: 40% ethanol extract was mixed evenly with aeration, the solid-liquid ratio was 1g:25mL, the extraction time was 2h, the extraction temperature was 50°C, and the supernatant was centrifuged at 3000r / min for 30min. The supernatant was allowed to flow steadily to remove bubbles and then precipitate. After precipitation, the precipitate and the supernatant were discharged in sequence through the bottom discharge port 5. The precipitate was reset to the reaction tank 1, and the reaction tank 1 was extracted and centrifuged at 3000r / min for 30min. The extraction time was 2h and the extraction temperature was 50°C. The extraction was repeated once, the supernatant was allowed to flow steadily to remove bubbles and then precipitate. After precipitation, the precipitate and the supernatant were discharged in sequence through the bottom discharge port 5.
[0068] Step 4, polyphenol extract: combine the two supernatants to obtain a polyphenol extract;
[0069] Step 5, concentration, purification, and elution: the polyphenol extract was concentrated by rotary evaporator, purified by AB-8 macroporous resin, and eluted with 50% ethanol solution;
[0070] Step 6: Powder making: Concentrate by rotary evaporator and freeze-dry in vacuum to constant mass to obtain fruit and vegetable polyphenol powder.
[0071] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A device for extracting polyphenols from fermented fruits and vegetables, comprising a reaction tank (1), wherein the reaction tank (1) is used for extracting polyphenols from fermented fruits and vegetables, and is characterized in that: A fermentation and extraction control mechanism (6) is rotatably provided in the middle of the reaction tank (1), a fermentation and extraction lifting control mechanism (7) is fixedly provided on one side of the reaction tank (1), and a rotation control mechanism (8) is fixedly provided at the bottom end of the fermentation and extraction control mechanism (6), wherein: The fermentation extraction control mechanism (6) comprises a positioning shaft (601) rotatable on the inner wall of the reaction tank (1), positioning bars (604) being fixedly provided on both sides of the outer wall of the positioning shaft (601), a positioning sleeve (605) being provided on the outer wall of the positioning shaft (601) through the positioning and sliding of the positioning bars (604), two stirring and crushing shafts (613) being fixedly provided on one end of the positioning sleeve (605), a limiting groove (606) being fixedly provided at the middle position of the outer wall of the positioning sleeve (605), a positioning seat (614) being fixedly provided at the bottom end of the outer wall of the positioning sleeve (605), and flow stabilizing frames (615) being fixedly provided on both sides of the positioning seat (614), a filter frame (602) being rotatably provided on the outer wall of the positioning shaft (601) through the limiting groove (606), a protective pad (603) being fixedly provided on the outer wall of the filter frame (602), and the filter frame (602) being in contact with the inner wall of the reaction tank (1) through the protective pad (603); The fermentation extraction lifting control mechanism (7) is fixedly connected to both ends of the top of the filter frame (602) to drive the filter frame (602) to lift; An air inlet pipe (607) is inserted through one end of the bottom of the outer wall of the reaction tank (1), and an extract feed pipe (608) is inserted through the other end of the bottom of the outer wall of the reaction tank (1). One end of the air inlet pipe (607) and one end of the extract feed pipe (608) are connected via a connecting vessel (609). A flow divider (611) is fixedly provided at the top of the connecting vessel (609). A connecting frame (610) is rotatably provided on the connecting vessel (609) through the flow divider (611) at the top. A plurality of connecting seats (612) are provided at the top of the connecting frame (610), and an auxiliary sealing mechanism (9) is fixedly provided at one end of each of the plurality of connecting seats (612); The rotation control mechanism (8) comprises a first limit seat (801) fixed at the bottom end of the positioning shaft (601) and a second limit seat (803) fixed at the middle position of the top of the connecting frame (610); a fixing sleeve (804) is fixedly provided at the top end of the second limit seat (803); a fixing block (805) is provided at the middle position of the fixing sleeve (804) for limiting sliding; a third limit seat (802) is fixedly provided at the top end of the fixing block (805); a plurality of slope limit blocks (807) are fixedly provided at the bottom end of the first limit seat (801) and the top end of the third limit seat (802); and the top end of the second limit seat (803) and the bottom end of the third limit seat (802) are connected via a return spring (806).
2. The device for extracting polyphenols from fermented fruits and vegetables according to claim 1, characterized in that: A first motor (4) is fixedly provided at the middle position of the top of the reaction tank (1), and an output end of the first motor (4) is fixedly connected to the top end of the positioning shaft (601).
3. The device for extracting polyphenols from fermented fruits and vegetables according to claim 1, characterized in that: One end of the top of the reaction tank (1) is connected to a feed port (2), one side of the outer wall of the reaction tank (1) is connected to a slag discharge port (3), and the bottom end of the reaction tank (1) is connected to a liquid discharge port (5).
4. The device for extracting polyphenols from fermented fruits and vegetables according to claim 3, characterized in that: The feed port (2), slag discharge port (3) and liquid discharge port (5) are all provided with control valves.
5. The device for extracting polyphenols from fermented fruits and vegetables according to claim 2, characterized in that: The fermentation extraction lifting control mechanism (7) comprises a control frame (701) fixed on one side of the reaction tank (1); a positioning screw (702) is rotatably provided in the middle position of the control frame (701); a lifting frame (704) is threadedly provided on one end of the outer wall of the positioning screw (702); one end of the outer wall of the lifting frame (704) is slidably connected to one end of the top of the control frame (701); a connecting ring (705) is fixedly provided on the top of the lifting frame (704); a lifting seat (706) is fixedly provided on one side of the connecting ring (705); both ends of the lifting seat (706) pass through the top of the reaction tank (1) and are respectively fixedly connected to the two ends of the top of the filter frame (602); and a protective ring (707) is fixedly provided in the middle position of the top of the lifting seat (706).
6. The device for extracting polyphenols from fermented fruits and vegetables according to claim 5, characterized in that: A second motor (703) is fixedly provided at the top end of the control frame (701), and an output end of the second motor (703) is fixedly connected to the top end of the positioning screw rod (702).
7. The device for extracting polyphenols from fermented fruits and vegetables according to claim 3, characterized in that: The height of the slag discharge port (3) is flush with the highest height at which the filter frame (602) is lifted.
8. The device for extracting polyphenols from fermented fruits and vegetables according to claim 1, characterized in that: The auxiliary sealing mechanism (9) comprises a positioning rod (901) fixed to the top end of the connecting frame (610); an adjustment seat (904) is inserted into one end of the outer wall of the positioning rod (901); a protective cover (905) is fixed to the top end of the adjustment seat (904); a sealing gasket (906) is fixed to the bottom end of the protective cover (905); a positioning spring (902) is inserted into the other end of the outer wall of the positioning rod (901); and a positioning block (903) is fixed to the top end of the positioning rod (901).
9. The device for extracting polyphenols from fermented fruits and vegetables according to claim 6, characterized in that: An intelligent control panel is fixedly provided on one side of the reaction tank (1), and the first motor (4) and the second motor (703) are both electrically connected to an external power supply via the intelligent control panel.
Citation Information
Patent Citations
Extraction device of sugarcane polyphenol
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Clean type biological fermentation yield increasing equipment
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