Microbiological detection and analysis device based on medical examination

By designing a microbial detection and analysis device including a heat source base and multiple sets of isolated culture areas, the problem that existing equipment cannot provide suitable environment for microorganisms in different temperatures is solved, and efficient microbial culture and cost reduction are achieved.

CN119709391APending Publication Date: 2025-03-28THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Patent Information

Application Number
CN202411883974.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-20
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

Existing microbial culture equipment cannot provide a suitable culture environment for microorganisms of different temperatures at the same time, resulting in inefficient microbial culture and increased costs.

Method used

A microbial detection and analysis device based on medical testing is designed, including a heat source base and multiple sets of isolated culture areas. The heating medium is distributed to each isolated culture area through a heat source flow tube to achieve fine temperature control.

Benefits of technology

The device can simultaneously provide a suitable culture environment for microorganisms of different temperatures, improve the efficiency of microorganisms and reduce the cost of culture.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a microbiological detection and analysis device based on medical examination, which comprises a heat source base, the heat source base comprises a bottom bin, the bottom bin is divided into a left cavity and a right cavity, a heating medium and a heating rod are stored in the left cavity of the bottom bin, and a liquid pump and a control box which are electrically connected are arranged in the right cavity of the bottom bin; a plurality of sets of isolated culture areas are distributed up and down above the left cavity of the bottom bin, and a heating disc and a rotating disc are arranged in each set of isolated culture area; the heat source base is arranged, the multiple sets of isolated culture areas are distributed on the heat source base up and down, the heat source circulation pipe is arranged between the isolated culture areas and the heat source base in a penetrating mode, and a heating medium located in the heat source base can be heated under the work of the heat source base; the heating medium is controlled to move into each isolated culture area along the heat source circulation pipe, and the quantity, time and the like of the heating medium such as water flowing to each isolated culture area are controlled.
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Description

Technical Field

[0001] The invention relates to the field of culture detection equipment, in particular to a microbial detection and analysis device based on medical examination. Background Art

[0002] In the medical field, microbial infection is an important cause of many diseases. Accurate and rapid detection and analysis of microorganisms is crucial for disease diagnosis, treatment planning, and disease monitoring.

[0003] At present, the detection of microorganisms includes many steps, such as sample collection, sample transportation and storage, sample pretreatment, and microbial detection. Among them, the method of microbial detection includes a culture-based detection method, that is, inoculating the pretreated sample into a suitable culture medium, and selecting different culture media according to the type of microorganism and the purpose of detection, such as nutrient agar for bacterial culture, Sabouraud culture medium for fungal culture, etc. The inoculated culture medium is placed in a suitable temperature and humidity for a certain period of time. Observe the growth of microorganisms on the culture medium, including the morphology, size, color, texture and other characteristics of the colony, and preliminarily determine the type of microorganism.

[0004] As can be seen from the above, after the sample is inoculated on the culture medium, the culture medium needs to be placed at a corresponding temperature and humidity for a period of time. Therefore, the culture medium needs to be placed in a corresponding device, and the temperature and humidity of the environment in which the culture medium is located need to be adjusted by the operation of the device. At present, there are many devices for creating a suitable temperature and humidity environment for the culture medium. For example, a Chinese patent with publication number CN117229882B discloses a constant temperature and humidity microbial incubator, which includes a box body and a culture dish. A carrier for carrying the culture dish is provided in the box body, and a grabbing mechanism for grabbing the culture dish is provided on one side of the carrier. A transfer mechanism is installed on one side of the box body, and the transfer mechanism includes a transfer cover that passes through the side wall of the box body, a transfer box is nested in the transfer cover and slidably connected to the transfer box, and a placement slot for accommodating the culture dish is provided in the transfer box. An electric telescopic cylinder is connected to the outer end of the transfer box. The culture dish is transferred to the transfer box by using the grabbing mechanism through the transfer cover that passes through the box body and the transfer box that is slidably sealed in the transfer cover, and then the airflow is recovered and exchanged through the ventilation component, and finally the culture dish is stored and retrieved, so as to avoid the incubator being connected to the external atmosphere and affecting the temperature and humidity environment, and ensure the stability of the culture environment inside the incubator.

[0005] Although the culture equipment provided by the above patents and other types of culture equipment can provide a suitable environment for the culture medium, they have some defects. For example, different microorganisms have different temperatures and humidity requirements for cultivation. For example, the culture temperature of yeast, which is also a medium-temperature microorganism, is about 25°C-30°C, and the most suitable growth temperature for Escherichia coli is about 37°C. This means that in the case that the existing culture equipment cannot provide culture chambers with different temperatures, in medical microbiological testing, microorganisms of different temperatures can only be cultured in sequence, which leads to low culture efficiency of different microorganisms and increases the cost of microbial culture. Summary of the invention

[0006] The purpose of the present invention is to provide a microbial detection and analysis device based on medical examination, aiming to improve the problem that equipment used for microbial cultivation cannot cultivate microorganisms of different temperatures at the same time.

[0007] The present invention is implemented as follows: a microbial detection and analysis device based on medical examination includes a heat source base, the heat source base includes a bottom bin, the bottom bin is divided into left and right chambers, a heating medium and a heating rod are stored in the left chamber of the bottom bin, and an electrically connected liquid pump and a control box are arranged in the right chamber of the bottom bin; a plurality of isolation culture areas are distributed above and below the left chamber of the bottom bin, a heating plate and a turntable are arranged in each isolation culture area, and the turntable supports the culture medium; a heat source circulation pipe is arranged between the bottom bin and the plurality of isolation culture areas, the bottom of the heat source circulation pipe is connected to the liquid pump, and the heating plate is arranged on the side of the heat source circulation pipe.

[0008] Preferably, the heat source circulation pipe includes two tube bodies, which are an inlet pipe and an outflow pipe respectively. A curved pipe is connected to the top of the inlet pipe and the outflow pipe, and the bottom of each pipe extends into the left cavity of the bottom bin. The curved pipe is a solid structure; the liquid inlet pipe of the liquid pump is connected to the left cavity of the bottom bin, and the liquid outlet end is connected to the inlet pipe.

[0009] Preferably, two groups of heating disks are provided, and the two groups of heating disks are respectively arranged above the turntable; the liquid inlet ends of the two sets of heating disks are connected to the inlet pipe through the solenoid valve, and the liquid outlet ends are connected to the outflow pipe through the solenoid valve; the solenoid valve is electrically connected to the control box.

[0010] Preferably, the isolation culture area also includes a cylinder and a top cover, the top of the cylinder is set to be an opening, and a first central tube is arranged through the bottom, the top cover is installed at the opening of the cylinder, and a fixed splint and a movable splint connected by bolts are arranged on the lower side, and the fixed splint and the movable splint are clamped on the first central tube.

[0011] Preferably, a second central tube is provided at the center of the left cavity of the bottom bin. Slots are provided at the tops of both the second central tube and the first central tube. An insertion tube is provided at the bottom of the first central tube, and the insertion tube is inserted into the adjacent slot. The heat source circulation tube is arranged inside the first central tube and the second central tube, and its bottom penetrates through the second central tube.

[0012] Preferably, a bracket is provided below the turntable. A connecting plate is connected below the middle of the bracket. An internal gear ring is connected to the inner side of the connecting plate. A gear is meshingly connected to the inner side of the internal gear ring, and the gear is installed on the output shaft of the motor.

[0013] Preferably, the bracket is sleeved on the first central tube through a bearing connection. The motor is installed inside the first central tube. A notch is provided on the side wall of the first central tube, and the gear penetrates through the notch. An industrial camera is provided above the turntable. The industrial camera is installed on the top cover and is electrically connected to the control box.

[0014] Preferably, an inlet and outlet is provided on the side wall of the cylinder body, and clamping grooves are provided at the upper and lower edges of the side wall. An access groove is communicated with the clamping groove. A closing device is provided at the inlet and outlet. The closing device includes a closing plate, a C-shaped plate and a threaded rod. Inclined surfaces are provided on the surfaces of the closing plate that are in contact with the inlet and outlet. Clamping columns are provided at both the upper and lower ends of the C-shaped plate, and the clamping columns are installed in the clamping grooves. The threaded rod penetrates through the C-shaped plate in a threaded manner, and its end is inserted into the closing plate.

[0015] Preferably, an adding mechanism is provided below the top cover. The adding mechanism includes an outer gas tube and an inner liquid tube. The liquid tube is located inside the gas tube, and nozzles are provided below both the gas tube and the liquid tube. An inlet tube is communicated with the side of the gas tube and the liquid tube respectively, and a solenoid valve is provided on the inlet tube. The inlet tube of the gas tube penetrates through the cylinder body and is connected to a gas cylinder, and the inlet tube of the liquid tube penetrates through the first central tube and is connected to the inflow tube of the heat source circulation tube.

[0016] Preferably, clamping arc plates are sleeved on both the gas tube and the liquid tube. A first snap plate is fixedly provided at the top of the clamping arc plate. A snap groove is provided on the lower side surface of the top cover, and the first snap plate is installed in the snap groove.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] 1. The present invention provides a heat source base, and multiple sets of isolation culture areas are vertically distributed above the heat source base. A heat source circulation tube is penetrated between the isolation culture areas and the heat source base. Under the operation of the heat source base, the heating medium located in the heat source base can be heated, and the heating medium can be controlled to move along the heat source circulation tube to each isolation culture area. By controlling the amount, time, etc. of the heating medium such as water flowing into each isolation culture area, and using the heat transfer principle to increase the temperature of the isolation culture area, a suitable environment for microorganism culture can be provided.

[0019] 2. The present invention sets a heating plate in the isolated culture area, and the inlet and outlet ends of the heating plate are connected to the inlet pipe and the outlet pipe of the heat source circulation pipe through an electromagnetic valve. The electromagnetic valve is operated to control the amount and time of the heating medium flowing to the heating plate, so as to realize the adjustment of the temperature of the isolated culture area.

[0020] 3. The present invention is provided with an industrial camera, which can be used to photograph the condition of the culture medium and display the growth status of the microorganisms through a display screen, providing convenience for the inspectors to preliminarily judge the types of microorganisms; at the same time, the turntable can rotate under the action of an external force, so different culture media can be controlled to move to the bottom of the industrial camera by rotating the turntable. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the structure of the heat source base, the heat source flow pipe, and the gas bottle of the present invention;

[0023] Figure 3 It is a structural schematic diagram of the heat source base of the present invention;

[0024] Figure 4 It is a partial structural schematic diagram of the heat source base of the present invention;

[0025] Figure 5 It is a first structural schematic diagram of the isolation culture area of ​​the present invention;

[0026] Figure 6 is a second structural schematic diagram of the isolation culture area of ​​the present invention;

[0027] Figure 7 It is a structural schematic diagram of the cylinder of the present invention;

[0028] Figure 8 It is a schematic diagram of the structure of the closing device of the present invention;

[0029] Fig. 9 It is a schematic diagram of the structure of the top cover of the present invention;

[0030] Fig.10 is a schematic structural diagram of the first central tube of the present invention;

[0031] Fig.11 It is a structural schematic diagram of the turntable of the present invention;

[0032] Fig.12 It is a structural schematic diagram of the adding mechanism of the present invention;

[0033] Fig.13 It is a structural schematic diagram of the clamping arc plate of the present invention;

[0034] Fig.14 It is a structural schematic diagram of the heating plate of the present invention.

[0035] In the figure: 1, isolated culture area; 11, top cover; 111, movable splint; 112, fixed splint; 113, sensor; 114, buckle slot; 12, closing device; 121, first insulation layer; 122, closing plate; 123, plate; 124, threaded rod; 125, clamping column; 13, cylinder; 131, second insulation layer; 132, inlet and outlet; 133, inlet and outlet slot; 134, clamping slot; 14, first central tube; 141, slot; 142, cannula; 143, notch; 15, heating plate; 151, solenoid valve; 16, adding mechanism; 161, gas pipe; 162, liquid pipe ; 163, nozzle; 164, arc clamping plate; 165, inlet pipe; 166, first snap plate; 17, industrial camera; 18, turntable; 181, bracket; 182, bearing; 183, connecting plate; 184, inner ring; 185, gear; 186, motor; 2, heat source base; 21, bottom bin; 211, second snap plate; 212, heating rod; 22, second center tube; 23, first closing cover; 24, second closing cover; 25, liquid pump; 26, control box; 27, heat dissipation hole; 28, support frame; 281, third snap plate; 3, heat source flow pipe; 31, elbow; 4, gas bottle. DETAILED DESCRIPTION

[0036] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] The following is a further description with reference to the accompanying drawings and specific embodiments:

[0038] Example 1

[0039] In order to simultaneously culture and process microorganisms growing at different temperatures in a variety of microbial tests and reduce the time and cost of microbial culture, this embodiment provides a new culture detection and analysis device. When using the culture device to culture microorganisms, the microorganisms can be placed in different chambers according to their suitable growth temperatures, and the temperature, humidity, etc. of the chamber can be adjusted to provide a suitable environment for microbial culture.

[0040] like Figure 1-3 , Figure 5-10As shown in Figures 1 and 14, specifically, the culture detection and analysis device includes a heat source base 2, a heat source circulation pipe 3, a plurality of isolated culture areas 1, etc. The plurality of isolated culture areas 1 are arranged above and below the heat source base 2, and the heat source circulation pipe 3 runs through the plurality of isolated culture areas 1, and the bottom extends into the heat source base 2. Under the operation of the heat source base 2, the water and other heating media stored inside it can be increased, and the water and other heating media are forced to flow along the heat source circulation pipe 3 to the plurality of isolated culture areas 1, and flow into the heat source base 2 again after flowing in the isolated culture area 1. By controlling the amount of water and other heating media flowing to each isolated culture area 1, the residence time, etc., and using the principle of heat transfer to increase the temperature of the isolated culture area 1, a suitable environment is provided for microbial culture. Because it is set as a plurality of isolated culture areas 1, it can provide respective culture spaces for microorganisms with different growth temperatures, thereby improving the culture efficiency of microorganisms with different growth temperatures.

[0041] In order to provide heat for multiple sets of isolated culture areas 1, the heat source base 2 includes a bottom bin 21, a heating rod 212, a liquid pump 25, etc. The bottom bin 21 is divided into two left and right chambers, and a first closed cover 23 and a second closed cover 24 are respectively provided on the left and right chambers, and a heat dissipation hole 27 is provided on the side wall of the right chamber. The heating rod 212 is laid on the bottom of the left chamber, and the end seal penetrates the side wall of the left chamber and extends into the right chamber. In addition, water and other heating media are also stored in the left chamber. Therefore, when the heating rod 212 is powered, heat can be generated to increase the temperature of the water and other heating media. The heating rod 212 is an existing device, which is briefly introduced here. It includes a tube shell, a heating wire located in the tube shell, and a temperature control component arranged at the end of the tube shell. An insulating material (such as ceramic, quartz, glass fiber, etc.) is provided at the junction of the temperature control component and the tube shell. The insulating material is an insulating material that separates the heating wire from the tube shell and the external environment. The temperature control component (including temperature sensor and control circuit) in this embodiment is located in the right cavity, and the control circuit includes signal amplification and processing circuit, comparator circuit, control element circuit (such as relay circuit), power supply circuit, etc. The temperature control component is connected to the control box 26 installed on the side wall of the right cavity through a wire.

[0042] The liquid pump 25 is arranged in the right cavity, the liquid inlet pipe of the liquid pump 25 is connected to the left cavity of the bottom bin 21, and the liquid outlet is connected to the heat source circulation pipe 3. Therefore, under the action of the liquid pump 25, the heating medium such as water after heating can be controlled to flow into the heat source circulation pipe 3, and the heating medium such as water is dispersed into each isolated culture area 1 through the heat source circulation pipe 3. In order to control the operation of the liquid pump 25, the liquid pump 25 is connected to the control box 26. The control box 26 is provided with a controller (such as PLC), a relay, a contactor, a power switch, a power module, a fuse, a terminal block, a wiring trough, etc., and the number is determined according to demand. The various devices in the above-mentioned control box 26 are connected by wires, and the specific connection method refers to the prior art. The power line of the liquid pump 25 is connected to the power output end of the control box 26, and at the same time, according to the output signal type of the control device (such as PLC, relay, etc.) in the control box, the corresponding signal line is selected to be connected to the control end of the liquid pump. For example, if the control device outputs a switching signal (such as a high level or a low level), an ordinary control cable can be used for connection; if the output is an analog signal (such as 4-20mA, 0-10V, etc.), a shielded cable must be used to reduce signal interference. The control signal line should be connected according to the wiring diagram of the control box and the liquid pump to ensure the correct transmission and control of the signal. In order to ensure the safe operation of the equipment and the safety of personnel, both the control box and the liquid pump need to be grounded. Connect the grounding terminal of the control box 26 and the grounding terminal of the liquid pump 25 to the grounding electrode through a grounding wire so that the outer casing of the equipment maintains good contact with the ground and any leakage current that may be generated is introduced into the ground in time.

[0043] In order to control the circulation of water and other heating media, the heat source circulation pipe 3 includes two pipe bodies, which are an inflow pipe and an outflow pipe respectively. The tops of the inflow pipe and the outflow pipe are connected with an elbow 31, which is a solid structure. When the inflow pipe and the outflow pipe are stably connected, the meaningless circulation of water and other heating media along the heat source circulation pipe 3 is cut off. The bottoms of the inflow pipe and the outflow pipe are both extended into the left cavity of the bottom bin 21, providing support for connecting the inflow pipe and the outflow pipe with each isolated culture area 1 to form a circulation path. The liquid outlet end of the liquid pump 25 is connected to the inflow pipe of the heat source circulation pipe 3 through a pipe joint, etc., so the circulation of water and other heating media can be controlled under the action of the liquid pump 25.

[0044] In order to receive heating media such as water, the isolated culture area 1 also includes a cylinder 13, a top cover 11, a heating disk 15 and a turntable 18. The top of the cylinder 13 is set to be open, and a first central tube 14 is provided through the bottom of the cylinder 13. The top cover 11 is installed at the opening of the cylinder 13 and is stably connected to the first central tube 14. At the same time, a sensor 113 is provided through the top cover 11. The sensor 113 includes a temperature sensor, a humidity sensor, etc., and is connected to the control box 26. The turntable 18 can be rotatably sleeved on the first central tube 14 for supporting the culture medium. There are two groups of heating disks 15, and the two groups of heating disks 15 are respectively arranged above the turntable 18.

[0045] The liquid inlet ends of the two sets of heating plates 15 are connected to the inflow pipe through the solenoid valve 151, and the liquid outlet ends are connected to the outflow pipe through the solenoid valve 151. The solenoid valve 151 is electrically connected to the control box 26. Therefore, after the control box 26 receives the temperature information transmitted by the sensor 113, the solenoid valve 151 can be controlled to work, so that the water or other heating medium flows from the inflow pipe of the heat source circulation pipe 3 to the heating plate 15, and flows back from the outflow pipe to the heat source circulation pipe 3. In the process of the circulation of the water or other heating medium, the heat is transferred to the space formed by the cylinder 13 and the top cover 11, thereby increasing the temperature of the space.

[0046] The connection between the solenoid valve 151, the sensor 113 and the control box 26 is a prior art. For example, the power line of the solenoid valve 151 is directly connected to the output terminal in the control box, or it can be connected by using an intermediate relay. In addition, the controller in the control box 26 can control the operation of the solenoid valve 151 by outputting high and low levels or on-off signals. For example, the sensor 113 can be connected to the control box 26 through a cable, one end of the cable is connected to the signal output end and the power input end of the sensor (if the sensor needs external power supply), and the other end is connected to the corresponding interface of the control box.

[0047] In order to facilitate the placement of culture medium, an inlet and outlet 132 is provided on the side wall of the cylinder 13, so that the arm of the inspector can pass through the inlet and outlet 132 to place the culture medium on the turntable 18, or remove the corresponding culture medium from the turntable 18. In order to enable the cylinder 13 to form a closed space, a closing device 12 can be adjusted at the inlet and outlet 132. The closing device 12 includes a closing plate 122. The surfaces where the closing plate 122 and the inlet and outlet 132 contact are both provided with inclined surfaces. Under the action of external force, the closing plate 122 can be controlled to be stably set at the inlet and outlet 132. In order to reduce the heat dissipation efficiency, a first insulation layer 121, a second insulation layer 131, etc. are respectively provided on the outside of the closing plate 122, the cylinder 13, and the top cover 11 to achieve heat insulation treatment of the space formed by the cylinder 13 and the top cover 11.

[0048] In order to realize the adjustable setting of the closing plate 122, a slot 134 is provided on the upper and lower edges of the side wall of the cylinder 13, and the central angle of the slot 134 is greater than 180°. An inlet and outlet groove 133 is provided on the slot 134. The closing device 12 also includes a plate 123 and a threaded rod 124. The upper and lower ends of the plate 123 are provided with a clamping column 125, and the clamping column 125 is installed in the slot 134 to realize the stable and movable setting of the plate 123 relative to the cylinder 13, so that the plate 123 can be pushed to move, and support is provided for adjusting the position of the closing plate 122. The threaded rod 124 is threadedly arranged through the plate 123, and the end is connected and inserted into the closing plate 122 through a bearing connection. Therefore, when the threaded rod 124 is rotated, the closing plate 122 can be controlled to move relative to the plate 123, so as to facilitate the closing plate 122 to block the inlet and outlet 132, or to make the inlet and outlet 132 unobstructed.

[0049] In order to make the top cover 11 and the first center tube 14 stably and detachably connected, a fixed clamp 112 is fixedly provided on the lower side of the top cover 11, and a movable clamp 111 is provided on the side of the fixed clamp 112 through bolts. The fixed clamp 112 and the movable clamp 111 are clamped and sleeved on the first center tube 14 to achieve the connection between the top cover 11 and the first center tube 14, and at the same time provide convenience for disassembling the top cover 11, the cylinder 13 and the first center tube 14 as needed.

[0050] In order to control the isolation culture area 1 to be stably and detachably connected to the heat source base 2, a second central tube 22 is provided at the center of the left cavity of the bottom bin 21, and slots 141 are provided at the tops of the second central tube 22 and the first central tube 14, and a plug 142 is provided at the bottom of the first central tube 14, and the plug 142 is inserted into the adjacent slot 141, and the first central tube 14 is connected to the second central tube 22 by bolts, so that under the action of the central tube, the isolation culture area 1 is stably connected to the heat source base 2. Similarly, in order to make adjacent isolation culture areas 1 stably and detachably connected, adjacent first central tubes 14 are connected with reference to the connection method between the first central tube 14 and the second central tube 22, so that multiple sets of isolation culture areas 1 are also provided with a detachable structure, which provides convenience for increasing or decreasing the number of isolation culture areas 1 according to needs.

[0051] In addition, the heat source circulation tube 3 is arranged in the first central tube 14 and the second central tube 22, and the bottom is arranged to pass through the second central tube 22. This arrangement prevents the heat source circulation tube 3 from affecting the operation of the isolated culture area 1.

[0052] Example 2

[0053] like Figure 6 Fig.10 , Fig.11As shown, on the basis of Example 1, in order to observe the growth of microorganisms in the culture medium, an industrial camera 17 is arranged above the turntable 18, the industrial camera 17 is mounted on the top cover 11, and is electrically connected to the control box 26. When the industrial camera 17 is working, the culture medium can be photographed, and the photographing information can be transmitted to the control box 26. A display screen is arranged on the side wall of the control box 26, and the growth of microorganisms can be displayed on the display screen after the information is retrieved. The connection method between the industrial camera 17 and the control box 26 here has also been disclosed. For example, the industrial camera 17 can be connected to the controller of the control box 26 through a USB interface and a cable, and the USB Vi s ion protocol is executed at the same time. The industrial camera converts the image data into a format that complies with the USB Vi s ion protocol and transmits it to the controller through the USB interface. And the graphics device interface (GDI) of the controller can be used to display the image information on the display. In addition, the controller can convert the image data received from the industrial camera into a format that can be recognized by GDI, and then draw and display it in a specified window or area of ​​the display by calling the GDI function. The controller can also be equipped with corresponding algorithms to process the acquired data and make a preliminary judgment on the growth of microorganisms, such as image preprocessing, feature extraction, target recognition and classification, data analysis and decision-making, and other publicly available algorithms.

[0054] In order to control multiple culture media to pass through the industrial camera 17, a bracket 181 is provided below the turntable 18. A connecting plate 183 of an annular structure can be connected below the middle of the bracket 181 by bolts or welding. An inner ring gear 184 is connected to the inner side of the connecting plate 183. A gear 185 is provided on the inner side of the inner ring gear 184 in meshing connection. The gear 185 is installed on the output shaft of a motor 186 (such as a stepping motor), and the motor 186 is connected to the control box 26. The bracket 181 is connected and sleeved on the first central tube 14 through a bearing 182. The motor 186 is installed on the inner side of the first central tube 14. A notch 143 is provided on the side wall of the first central tube 14. The gear 185 is provided through the notch 143. Therefore, under the operation of the motor 186, the inner ring gear 184 can be controlled to drive the turntable 18 to rotate, forcing the culture media to move to the bottom of the industrial camera 17. Here, the connection method between the motor 186 and the control box 26 has also been disclosed, for example, the motor and the control box are mainly connected by a cable.

[0055] Example 3

[0056] like Figure 6 , Fig.12 , Fig.13As shown, on the basis of Example 1 or 2, in order to be able to adjust the humidity in the space composed of the cylinder 13 and the top cover 11, an adding mechanism 16 is arranged below the top cover 11, and the adding mechanism 16 includes a gas pipe 161 and a liquid pipe 162 arranged inside and outside, the liquid pipe 162 is located on the inner side of the gas pipe 161, and nozzles 163 are arranged below the gas pipe 161 and the liquid pipe 162, and inlet pipes 165 are respectively arranged on the sides of the gas pipe 161 and the liquid pipe 162, and a solenoid valve is arranged on the inlet pipe 165, the inlet pipe 165 of the gas pipe 161 passes through the cylinder 13 and is connected to the gas bottle 4, and the inlet pipe 165 of the liquid pipe 162 passes through the first central pipe 14 and is connected to the inflow pipe of the heat source circulation pipe 3. When the humidity is low, the control box 26 can control the electromagnetic valve to work, so that the liquid pipe 162 is unobstructed, and then the water in the heat source flow pipe 3 is sprayed into the space formed by the cylinder 13 and the top cover 11 through the nozzle 163, changing the humidity of the space. Of course, if aerobic microorganisms are to be cultivated, the gas bottle 4 can be opened to inject oxygen into the space formed by the top cover 11 and the cylinder 13 to provide a good environment for the growth of aerobic microorganisms.

[0057] In order to stably install the gas pipe 161 and the liquid pipe 162 on the top cover 11, a clamping arc plate 164 is sleeved on the gas pipe 161 and the liquid pipe 162, a first snap plate 166 is fixedly arranged on the top of the clamping arc plate 164, a snap groove 114 is arranged on the lower side of the top cover 11, and the first snap plate 166 is installed in the snap groove 114, so as to realize the detachable installation of the clamping arc plate 164. After the clamping arc plate 164 is installed on the top cover 11, under the action of the gas pipe 161 and the liquid pipe 162, a plurality of the clamping arc plates 164 are gathered together, so that the clamping arc plates 164 are stably installed.

[0058] In order to ensure the stable installation of the gas cylinder 4 , a support frame 28 is sleeved on the bottom of the gas cylinder 4 , a third snap plate 281 is fixedly provided on the support frame 28 , and a second snap plate 211 is provided on the bottom bin 21 , and the third snap plate 281 is installed in the second snap plate 211 .

[0059] Of course, a lamp tube can also be arranged under the top cover 11, and the lamp tube can be equipped with corresponding auxiliary devices (such as ballast, driver, lamp holder, dimmer, etc.), and the top cover 11 can be connected to the control box 26 through the auxiliary devices to provide light for the cultivation of microorganisms that need light.

[0060] In addition, an air pump may be provided in the right chamber of the bottom bin 21, and an exhaust pipe with a valve body may be provided on each cylinder 13, so that the air pump can be used to extract oxygen from the cylinder 13, thereby providing a good environment for the growth of anaerobic organisms.

[0061] The laying of cables connecting the above-mentioned devices is determined according to actual needs during production, and the laying of cables is completed using existing public technologies.

[0062] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microbial detection and analysis device based on medical examination, characterized in that: The invention comprises a heat source base (2), wherein the heat source base (2) comprises a bottom bin (21), wherein the bottom bin (21) is divided into two left and right chambers, wherein a heating medium and a heating rod (212) are stored in the left chamber of the bottom bin (21), and an electrically connected liquid pump (25) and a control box (26) are arranged in the right chamber of the bottom bin (21); a plurality of isolation culture areas (1) are arranged in upper and lower distribution above the left chamber of the bottom bin (21), wherein a heating plate (15) and a rotating plate (18) are arranged in each isolation culture area (1), wherein the rotating plate (18) supports a culture medium; a heat source circulation pipe (3) is arranged between the bottom bin (21) and the plurality of isolation culture areas (1), wherein the bottom of the heat source circulation pipe (3) is connected to the liquid pump (25), and the heating plate (15) is arranged on the side of the heat source circulation pipe (3).

2. A microbial detection and analysis device based on medical examination according to claim 1, characterized in that: The heat source circulation pipe (3) comprises two pipe bodies, the two pipe bodies are an inlet pipe and an outlet pipe respectively, the tops of the inlet pipe and the outlet pipe are connected with a curved pipe (31), and the bottoms of both extend into the left cavity of the bottom bin (21), and the curved pipe (31) is a solid structure; the liquid inlet pipe of the liquid pump (25) is connected to the left cavity of the bottom bin (21), and the liquid outlet end is connected to the inlet pipe.

3. A microbial detection and analysis device based on medical examination according to claim 2, characterized in that: The heating discs (15) are provided in two groups, and the two groups of heating discs (15) are respectively arranged above the rotating disc (18); the liquid inlet ends of the two sets of heating discs (15) are connected to the inlet pipe through the electromagnetic valve (151), and the liquid outlet ends are connected to the outlet pipe through the electromagnetic valve (151); the electromagnetic valve (151) is electrically connected to the control box (26).

4. A microbial detection and analysis device based on medical examination according to claim 1, characterized in that: The isolation culture area (1) also includes a cylinder (13) and a top cover (11); the top of the cylinder (13) is arranged to be open, and a first central tube (14) is arranged through the bottom; the top cover (11) is installed at the opening of the cylinder (13), and a fixed clamp (112) and a movable clamp (111) connected by bolts are arranged on the lower side; the fixed clamp (112) and the movable clamp (111) are clamped and sleeved on the first central tube (14).

5. A microbial detection and analysis device based on medical examination according to claim 4, characterized in that: A second central tube (22) is arranged at the center of the left cavity of the bottom bin (21); slots (141) are arranged at the tops of the second central tube (22) and the first central tube (14); a plug (142) is arranged at the bottom of the first central tube (14); the plug (142) is inserted into an adjacent slot (141); the heat source circulation tube (3) is arranged in the first central tube (14) and the second central tube (22), and the bottom penetrates the second central tube (22).

6. A microbial detection and analysis device based on medical examination according to claim 4, characterized in that: A bracket (181) is provided below the rotating disk (18), a connecting plate (183) is provided below the middle of the bracket (181), an inner gear ring (184) is connected to the inner side of the connecting plate (183), a gear (185) is provided on the inner side of the inner gear ring (184) in meshing connection, and the gear (185) is mounted on the output shaft of the motor (186).

7. A microbial detection and analysis device based on medical examination according to claim 6, characterized in that: The bracket (181) is connected and sleeved on the first central tube (14) through a bearing (182), the motor (186) is installed on the inner side of the first central tube (14), a notch (143) is provided on the side wall of the first central tube (14), and the gear (185) is provided through the notch (143); an industrial camera (17) is provided above the turntable (18), the industrial camera (17) is installed on the top cover (11), and is electrically connected to the control box (26).

8. The microorganism detection and analysis device based on medical examination according to claim 4, characterized in that: An inlet and outlet (132) is provided on the side wall of the cylinder (13), and a slot (134) is provided on the upper and lower edges of the side wall, and an inlet and outlet groove (133) is provided on the slot (134); a closing device (12) is provided at the inlet and outlet (132), and the closing device (12) includes a closing plate (122), a locking plate (123) and a threaded rod (124); the contact surfaces of the closing plate (122) and the inlet and outlet (132) are provided with inclined surfaces, and the upper and lower ends of the locking plate (123) are provided with locking columns (125), and the locking columns (125) are installed in the slot (134); the threaded rod (124) is threadedly penetrated through the locking plate (123), and the end portion is inserted into the closing plate (122).

9. The microorganism detection and analysis device based on medical examination according to claim 4, characterized in that: An adding mechanism (16) is arranged below the top cover (11), the adding mechanism (16) comprising a gas pipe (161) and a liquid pipe (162), the liquid pipe (162) being located inside the gas pipe (161), and nozzles (163) are arranged below the gas pipe (161) and the liquid pipe (162); inlet pipes (165) are arranged on the sides of the gas pipe (161) and the liquid pipe (162), and a solenoid valve (151) is arranged on the inlet pipe (165); the inlet pipe (165) of the gas pipe (161) passes through the cylinder (13) and is connected to the gas bottle (4), and the inlet pipe (165) of the liquid pipe (162) passes through the first central pipe (14) and is connected to the inflow pipe of the heat source circulation pipe (3).

10. A microbial detection and analysis device based on medical examination according to claim 9, characterized in that: The gas pipe (161) and the liquid pipe (162) are both sleeved with a clamping arc plate (164), a first snap plate (166) is fixedly arranged on the top of the clamping arc plate (164), a snap groove (114) is arranged on the lower side of the top cover (11), and the first snap plate (166) is installed in the snap groove (114).

Citation Information

Patent Citations

  • A constant temperature and humidity microbial incubator

    CN117229882B