Aspergillus niger and application thereof, and composite microbial phosphate-solubilizing bacterial agent and application thereof

Abstract

The invention relates to the technical field of microorganism application, and particularly discloses aspergillus niger and application thereof, and a compound microorganism phosphate-solubilizing bacterium agent and application thereof. The preservation number of the aspergillus niger is CGMCC (China General Microbiological Culture Collection Center) The invention provides the strain as well as a culture, fermentation liquor and a microbial inoculum thereof. In addition, the invention further provides a compound microorganism phosphate solubilizing bacterium agent containing the aspergillus niger and the arbuscular mycorrhizal fungi and application of the compound microorganism phosphate solubilizing bacterium agent to conversion of phosphorus which is fixed and difficult to absorb in soil into phosphorus which can be absorbed and utilized by crops. The Aspergillus niger and arbuscular mycorrhizal fungus composite microbial phosphate solubilizing bacterial agent provided by the invention can convert phosphorus which is easy to fix and difficult to absorb in soil into phosphorus which is easy to absorb, so that the waste of phosphorus in the soil is avoided, and more phosphorus elements which can be absorbed and utilized are provided for crops.

Description

Technical Field

[0001] The present application relates to the technical field of microbial application, and more specifically, to an Aspergillus niger and its application, and a composite microbial phosphate-solubilizing agent and its application. Background Art

[0002] Phosphorus is one of the macronutrients required for plant growth and development. It is a component of important substances such as nucleic acids, nucleoproteins, phospholipids, ATP, plant growth hormones and coenzymes. It plays an important role in photosynthesis, nitrogen metabolism, enzymatic reactions and energy metabolism. Phosphorus fertilizer applied to the soil is mainly orthophosphate, which is easy to react with Fe in the soil. 3+ 、Al 3+ The phosphorus fertilizer is fixed in the soil after forming complexes, which makes it less mobile and difficult for crops to absorb and utilize. Therefore, the absorption and utilization rate of phosphorus fertilizer by crops in the current season is only 5-15%. Even if the absorption and utilization of crops in the next season is taken into account, the absorption and utilization rate of phosphorus fertilizer is only 25%. Therefore, it is urgent to improve the mobility of phosphorus in the soil, reduce the loss of phosphorus fertilizer, and improve the absorption and utilization rate of phosphorus by crops.

[0003] There are many microorganisms in the soil that have the ability to solubilize phosphorus. They can convert phosphorus in the soil that is difficult for crops to absorb and utilize into phosphorus forms that can be absorbed and utilized by crops, thereby improving the phosphorus nutrition of crops. At the same time, they can also promote the metabolic activities of beneficial microorganisms in the soil.

[0004] Among them, microorganisms with phosphate-solubilizing ability include bacteria, fungi and actinomycetes. At present, there has been a lot of research on bacteria with phosphate-solubilizing ability in soil. In contrast, there are fewer reports on fungi with phosphate-solubilizing ability. Relevant evidence shows that the phosphate-solubilizing ability of fungi with phosphate-solubilizing ability is generally stronger than that of bacteria with phosphate-solubilizing ability, and their genetic traits are more stable, which has great potential in the application of microbial fertilizers.

[0005] Therefore, in order to provide crops with more absorbable and usable phosphorus, it is necessary to enrich the microorganisms in the soil that have the ability to solubilize phosphorus and explore more ways to achieve the transformation of phosphorus in the soil. Summary of the invention

[0006] The present application provides an Aspergillus niger and its application, a composite microbial phosphate-solubilizing agent and its application. The Aspergillus niger and the composite microbial phosphate-solubilizing agent provided in the present application can convert phosphorus elements in the soil that are difficult for crops to absorb and utilize into phosphorus elements that can be absorbed and utilized by crops, and can provide crops with more absorbable phosphorus elements, thereby promoting the growth of crops.

[0007] In a first aspect, the present application provides an Aspergillus niger, using the following technical solution:

[0008] The Aspergillus niger provided in this application was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on June 23, 2022, with the deposit number CGMCC No.40231, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing.

[0009] The strain was isolated from a soil sample from the "Youpan 7" peach orchard in Pinggu District, Beijing. The mycelium of the strain is initially white and dense; the spore-producing area in the old center is brown-black with a velvety texture, and the back is colorless. The strain can convert the phosphorus form in the soil that is difficult for crops to absorb and utilize into the phosphorus form that crops can absorb and utilize, thereby promoting the growth of crops.

[0010] In a second aspect, the present application provides a culture comprising the above-mentioned Aspergillus niger.

[0011] In a third aspect, the present application provides a fermentation broth comprising the above-mentioned Aspergillus niger.

[0012] In a fourth aspect, the present application provides a spore suspension comprising the spores of Aspergillus niger.

[0013] In a fifth aspect, the present application provides a bacterial agent, which includes the above-mentioned Aspergillus niger.

[0014] In a sixth aspect, the present application provides a bacterial powder comprising the above-mentioned Aspergillus niger.

[0015] In a seventh aspect, the present application provides a composite microbial phosphate-solubilizing agent, which adopts the following technical solution:

[0016] A composite microbial phosphate-solubilizing agent comprises an Aspergillus niger agent prepared by using the Aspergillus niger and an arbuscular mycorrhizal fungal agent prepared by using Pipepophysalis mosseae; the preservation number of Pipepophysalis mosseae is CGMCC No.40631.

[0017] The composite microbial phosphate-solubilizing agent provided in the present application can effectively convert phosphorus in the soil that is difficult for crops to absorb and utilize into phosphorus that can be absorbed and utilized by crops. By compounding the Aspergillus niger agent with the arbuscular mycorrhizal fungus agent, the conversion of phosphorus can be further improved, thereby further promoting the growth of crops.

[0018] Optionally, the effective viable count of the Aspergillus niger agent is ≥ 1×10 8 Pieces / g.

[0019] Optionally, the spore count of the arbuscular mycorrhizal fungal agent is ≥70 / g.

[0020] Optionally, the usage amount of the Aspergillus niger agent accounts for 5-15% of the total amount of the composite microbial phosphate-solubilizing agent; the usage amount of the arbuscular mycorrhizal fungi agent accounts for 15-25% of the total amount of the composite microbial phosphate-solubilizing agent; and the remainder is additives.

[0021] Optionally, the additive is oyster shell powder, humic acid powder or diatomaceous earth.

[0022] In an eighth aspect, the present application provides an application of the above-mentioned Aspergillus niger, culture, fermentation broth, spore suspension, bacterial agent or bacterial powder and composite microbial phosphate-solubilizing agent in converting phosphorus forms in the soil that are difficult for crops to absorb and utilize into phosphorus forms that can be absorbed and utilized by crops.

[0023] In summary, this application has the following beneficial effects:

[0024] 1. The present application provides an Aspergillus niger strain, which was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on June 23, 2022, with the deposit number CGMCC No.40231.

[0025] 2. The Aspergillus niger can convert phosphorus in the soil that is difficult for crops to absorb and utilize into phosphorus that can be absorbed and utilized by crops, thereby promoting the growth of crops.

[0026] 3. The present application also provides a composite microbial phosphate-solubilizing agent, which includes the Aspergillus niger agent prepared by the Aspergillus niger and the arbuscular mycorrhizal fungal agent prepared by using Pipepophysalis mosseae; the preservation number of Pipepophysalis mosseae is CGMCC No.40631.

[0027] 4. The composite microbial phosphate-solubilizing agent can effectively convert the phosphorus form in the soil that is difficult for crops to absorb and utilize into the phosphorus form that can be absorbed and utilized by crops. By compounding the Aspergillus niger agent with the arbuscular mycorrhizal fungus agent, the conversion of phosphorus can be further improved, thereby further promoting the growth of crops. DETAILED DESCRIPTION

[0028] Before describing the embodiments of the present application in detail, it should be understood that the terms used herein are only used for the purpose of describing specific embodiments. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by ordinary technicians in the field to which the terms belong.

[0029] The endpoints and any values ​​of the ranges disclosed in this application are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0030] In the present application, the terms "comprise" or "include" are open expressions, that is, including the contents specified in the present application but not excluding other contents.

[0031] The present application provides an Aspergillus niger strain, which was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on June 23, 2022, with a deposit number of CGMCC No. 40231. In addition, the present application also provides a method for isolating, purifying and culturing the strain. And provides a culture, fermentation broth, spore suspension, bacterial powder, and bacterial agent comprising the strain.

[0032] The present application also provides the use of the above-mentioned Aspergillus niger and the culture, fermentation liquid, spore suspension, bacterial powder and bacterial agent comprising the strain in converting the phosphorus form in the soil that is difficult for crops to absorb and utilize into the phosphorus form that can be absorbed and utilized by crops.

[0033] The present application also provides a composite microbial phosphate-solubilizing agent. The composite microbial phosphate-solubilizing agent includes an Aspergillus niger agent prepared using the Aspergillus niger and an arbuscular mycorrhizal fungal agent prepared using Pipepodactylus mosesi; the Pipepodactylus mosesi deposit number is CGMCC No.40631. The effective viable count of the Aspergillus niger agent is ≥1×10 8 / g; the spore number of arbuscular mycorrhizal fungi agent is ≥70 / g.

[0034] The present application also provides the above-mentioned composite microbial phosphate-solubilizing agent, which can effectively convert phosphorus in the soil that is difficult for crops to absorb and utilize into phosphorus that can be absorbed and utilized by crops. By compounding the Aspergillus niger agent with the arbuscular mycorrhizal fungus agent, the conversion of phosphorus can be further improved, thereby further promoting the growth of crops.

[0035] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work belong to the scope of protection of the present application. The embodiments described below are exemplary and are only used to explain the present application, and cannot be interpreted as limiting the present application.

[0036] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0037] In the following embodiments, the formula of the PDA solid culture medium involved is as follows: based on a volume of 1L, it includes 200g peeled potatoes, 20g glucose, 15-20g agar, 1000mL water, and a pH of 7.0-75; the formula of the PDA liquid culture medium involved is as follows: based on a volume of 1L, it includes 200g potatoes, 20g glucose, 1000mL water, and a pH of 7.0-75.

[0038] The present application is further described in detail below in conjunction with the embodiments and test results.

[0039] Example

[0040] Example 1

[0041] This example provides a new bacterial strain separation and purification process.

[0042] The details are as follows:

[0043] (1) Soil sample collection: Soil was collected from the “Youpan 7” peach orchard in Pinggu District, Beijing, and stored at 4°C before being sent to the laboratory.

[0044] (2) Isolation of target bacteria: Weigh 10 g of the soil sample collected above and add it to a pre-sterilized Erlenmeyer flask containing 90 ml of sterile water under aseptic conditions. Place it at 28°C and 150 rpm for 30 min, let it stand for 15 min, and dilute it to 10 -6 , select a dilution concentration of 10 -4 , 10 -5 , 10 -6 The dilutions were spread on PDA solid culture medium plates, and after the liquid was absorbed, the coated PDA solid culture medium plates were placed inverted and cultured at 28°C for 48-72h.

[0045] Pick a single colony on the plate, streak it onto a new PDA solid culture medium plate, and place the streaked PDA solid culture medium plate in a constant temperature incubator at 28°C for 48-72 hours; observe that there is only one colony morphology in the PDA solid culture medium plate after incubation, indicating that the separation and purification is complete; store it for future use.

[0046] (3) Preliminary identification: The isolated and purified strain was examined and observed under a microscope. Based on the results of the microscopic examination, it was preliminarily determined that the strain was a fungus of the genus Aspergillus.

[0047] Example 2

[0048] This example provides the properties of the strain isolated and purified in Example 1 and the results of 16S rDNA sequencing. The details are as follows:

[0049] (I) Strain properties

[0050] (1) Morphological characteristics

[0051] The strain was cultured on a PDA plate at 28°C for 72 hours and had a diameter of 2.5-3.5 cm. The hyphae were initially white and dense; the old central spore-producing area was brown-black with a velvety texture and the back was colorless.

[0052] (2) Cultivation characteristics

[0053] The optimal growth conditions for the strain are: pH=7.0-7.5, temperature of 25-28°C, and culture time of 48-72h.

[0054] (3) Functional characteristics

[0055] This strain can convert phosphorus in the soil that is difficult for crops to absorb and utilize into phosphorus that can be absorbed and utilized by crops, thereby promoting crop growth.

[0056] (4) The strain was identified by 16S rDNA sequencing technology, and the 16S rDNA sequence of the strain was obtained and the sequence was compared. The phylogenetic tree was constructed by the neighbor-joining method using MEGA5.0 software to determine the position of the strain in the phylogenetic tree. The identification and comparison results showed that the strain was Aspergillus niger and was named Aspergillus niger JP01.

[0057] Aspergillus niger was deposited in the General Microbiology Center of China Microorganism Culture Collection on June 23, 2022, with the deposit number CGMCC No.40231, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing.

[0058] Example 3

[0059] This embodiment provides a fermentation broth of Aspergillus niger.

[0060] The preparation method of the above fermentation liquid is as follows:

[0061] The strain isolated and purified in Example 1 was activated, transferred to a PDA liquid culture medium with a pH of 7.0, and cultured at 28° C. for 48 h to obtain a fermentation broth of Aspergillus niger.

[0062] Example 5

[0063] This embodiment provides a spore suspension of Aspergillus niger.

[0064] The preparation method of the above spore suspension is as follows:

[0065] The strain isolated and purified in Example 1 was activated. After the culture medium was covered with spores, sterile distilled water (normal saline or PBS buffer) was injected into the culture medium on a clean bench. The surface of the culture medium was gently scraped with a sterile inoculating loop to fully elute the spores. The culture medium was mixed and filtered through a sterile clean filter paper and an ordinary funnel (to remove non-spore substances). The filtrate was placed in a sterile clean small beaker and the filtrate was taken to obtain a spore suspension.

[0066] Example 6

[0067] This embodiment provides a fungal agent of Aspergillus niger.

[0068] The preparation process of the above-mentioned microbial agent is as follows:

[0069] Using the activated strain plate, the Aspergillus niger isolated and purified in Example 1 was eluted to prepare a spore suspension, and the spore count was performed using a hemocytometer. The spore count was 1×10 8 pcs / mL, for future use.

[0070] The crushed corn stalks were used as carbon source, sterilized by dry heat at 400℃ for 2h, cooled and collected for later use. The inoculation amount was 10% (v / v), the spore suspension was aspirated onto the corn stalk matrix, and fermented at 28℃ for 7d. The fermented bacteria and matrix were crushed and mixed evenly to obtain the Aspergillus niger inoculum.

[0071] Example 8

[0072] This embodiment provides an arbuscular mycorrhizal fungal agent.

[0073] The preparation method of the above-mentioned arbuscular mycorrhizal fungal agent is specifically as follows:

[0074] (1) Preparation of arbuscular mycorrhizal fungal inoculants

[0075] The arbuscular mycorrhizal fungal inoculant is prepared by using Pipestilens mosseae, wherein Pipestilens mosseae is a currently disclosed fungus species with a preservation number of CGMCC No.40631.

[0076] The specific method is as follows:

[0077] Sweet sorghum seeds were used as host plants, and the potted plants (the substrate was sandy soil: corn stalks (g / g) = 3:1) were inoculated with the inoculum of seeds and inoculant (g / g) = 1:1, and the sorghum plants were cultured. During the culture process, alternating dry and wet conditions were ensured, and the water content was not too high. After the sorghum plants were cultured in the potted plants for 2 months, watering was stopped in the potted plants, and drying promoted spore formation.

[0078] After drying for one week, the part of the sorghum plant exposed outside the substrate was cut off along the surface of the potting substrate, and all the cultures in the pot were collected to obtain the arbuscular mycorrhizal fungal inoculant.

[0079] (2) Preparation of arbuscular mycorrhizal fungal agents

[0080] The arbuscular mycorrhizal fungi inoculant and the arbuscular mycorrhizal fungi culture matrix prepared in step (1) are used to prepare an arbuscular mycorrhizal fungi inoculant, wherein the arbuscular mycorrhizal fungi culture matrix is ​​a mixture of sand and biochar (corn stalks), and the volume ratio of the two is 3:1.

[0081] The specific method is as follows:

[0082] The arbuscular mycorrhizal fungi inoculant prepared in step (1) is inoculated into the arbuscular mycorrhizal fungi culture matrix at an inoculation rate of seeds to inoculant (g / g) = 1:1, and mixed to prepare a propagation matrix containing arbuscular mycorrhizal fungi.

[0083] The propagation matrix containing arbuscular mycorrhizal fungi is spread on the surface of sandy soil in the sorghum field with a thickness of 10-20 cm, watered to 50-70% of the field water holding capacity, and sorghum seeds are sown.

[0084] After sowing, the surface is covered again with a propagation matrix containing arbuscular mycorrhizal fungi with a thickness of 1-2 cm.

[0085] The sorghum seeds were cultured for 2-3 months. During the culture process, the Hoagland nutrient solution was used for irrigation. At the same time, water was applied according to the humidity of the substrate, and the moisture content of the substrate was controlled to be 30-70% of the field water holding capacity.

[0086] After sorghum has grown for 2-3 months, the above-ground parts of the sorghum plants are removed along the field soil surface, the propagation matrix containing arbuscular mycorrhizal fungi and the sorghum plant roots are collected, the sorghum plant roots are cut into pieces, and the roots are mixed with the propagation matrix containing arbuscular mycorrhizal fungi to obtain the arbuscular mycorrhizal fungal agent.

[0087] Example 9

[0088] This embodiment provides an Aspergillus niger agent.

[0089] The preparation method of the above-mentioned Aspergillus niger agent specifically comprises the following steps:

[0090] (1) Preparation of Aspergillus niger spore solution

[0091] Aspergillus niger spore liquid was prepared by using Aspergillus niger, wherein the Aspergillus niger was the strain isolated and purified in Example 1 of the present application, and its deposit number was CGMCC No.40231.

[0092] The specific method is as follows:

[0093] Take the activated Aspergillus niger and inoculate it on the PDA solid medium (slant), culture it at 28°C, and after mature spores grow, repeatedly blow and rinse the slant with physiological saline to suspend the Aspergillus niger spores in the physiological saline to obtain the Aspergillus niger spore liquid. Adjust the OD of the Aspergillus niger spore suspension 600 =2.0 and transfer to a sterile container for later use.

[0094] (2) Preparation of Aspergillus niger fungus agent

[0095] The Aspergillus niger inoculant is prepared by using the Aspergillus niger spore liquid and a carbon source obtained in step (1). In this embodiment, the carbon source is a corn stalk culture medium.

[0096] The specific method is as follows:

[0097] The crucible filled with corn stalk powder was placed in a dry heat sterilizer. In order to minimize the oxygen content in the crucible, the sludge was compacted and a lid was placed on the crucible. After being treated at 400°C for 2 hours, the temperature was reduced to 50°C and the crucible was taken out. After cooling, the corn stalk powder was rinsed with deionized water to remove the attached small particles. After washing, the corn stalk powder was collected and dried with a freeze dryer, ground through a 60-mesh sieve, and the corn stalk culture matrix was obtained and sealed for storage.

[0098] The Aspergillus niger spore liquid was inoculated onto the corn straw culture matrix at an inoculation rate of 10%, and the mixture was placed at 28° C. for 7 days for static fermentation. After the fermentation was completed, the completely fermented bacteria and the corn straw culture matrix were crushed and stirred evenly to obtain the Aspergillus niger inoculant.

[0099] Example 10

[0100] This embodiment provides a composite microbial phosphate-solubilizing agent. The composite microbial phosphate-solubilizing agent includes the arbuscular mycorrhizal fungal agent prepared in Example 8 and the Aspergillus niger agent prepared in Example 9. The number of spores of the arbuscular mycorrhizal fungal agent is ≥ 70 / g, and the number of effective live bacteria of the Aspergillus niger agent is ≥ 1×10 8 Pieces / g.

[0101] The preparation process of the composite microbial phosphate-solubilizing agent is as follows: the arbuscular mycorrhizal fungi agent, the Aspergillus niger agent and the oyster shell powder are mixed in a weight ratio of 2:0.5:7.5 to obtain the composite microbial phosphate-solubilizing agent.

[0102] Examples 11-16

[0103] Examples 11-16 respectively provide a composite microbial phosphate-solubilizing agent. The difference between the above examples and Example 10 is that the weight percentage of each component added and the type of additive are specifically shown in Table 1, and the rest are consistent with Example 10.

[0104] The differences between Examples 8-16 are as follows:

[0105] Example 8 provides an arbuscular mycorrhizal fungal agent.

[0106] Example 9 provides an Aspergillus niger agent.

[0107] The difference between Examples 10-12 lies in the weight percentage of the arbuscular mycorrhizal fungi agent added.

[0108] The difference between Examples 13-14 and Example 11 is the weight percentage of the added Aspergillus niger agent.

[0109] The difference between Examples 15-16 and Example 11 is that the types of additives are different.

[0110] Table 1 Type and dosage of each component in the bacterial agent provided in the examples and comparative examples

[0111]

[0112] Comparative Example

[0113] This comparative example provides a fertilizer. The difference between this comparative example and Example 11 is that the fertilizer only includes oyster shell powder, and does not include arbuscular mycorrhizal fungi and Aspergillus niger agents, as shown in Table 1, and the rest is consistent with Example 11.

[0114] Performance testing

[0115] (1) Activation The Aspergillus niger strain (deposit number: CGMCC No. 40231) obtained by separation and purification in Example 1 of the present application was spotted on an inorganic phosphorus (tricalcium phosphate) solid plate and cultured at 28° C. for 7 days to observe whether a phosphate decomposition ring appeared.

[0116] After 7 days of initial screening culture at 28℃, it was found that Aspergillus niger could produce phosphate solubilization circles on inorganic phosphorus medium. As the culture time increased, the phosphate solubilization circle gradually increased and reached the maximum value on the 6th day.

[0117] (2) The products provided in Examples 8-16 and the comparative examples were used to carry out corn pot plant experiments to explore their effects on corn growth. The details are as follows:

[0118] Experimental setting: A total of 12 treatments were set up, namely: application of Ames + Xiaobudian 16-16-16 fertilizer and the composite microbial phosphate-solubilizing agent provided in Example 8-16, and the application of Arms + Xiaobudian 16-16-16 fertilizer and the product provided in the comparative example, only Ames + There were 3 replicates for each treatment, including 16-16-16 fertilizer and a blank control without treatment.

[0119] The test method is as follows: corn seeds are sown in pots (size: upper diameter 15 cm, height 20 cm; substrate: 2 kg soil (random soil) mixed with 2 g phosphate rock powder and 20 g of the product provided in the embodiment or comparative example) at a sowing rate of 4 seeds / g, and placed at 25°C, 50% RH for cultivation. When the corn seedlings grow to 3 cotyledons, the seedlings are thinned to 2 plants. After 30 days, the height of the corn plant, the root surface area, the aboveground dry weight, and the total phosphorus of the plant are measured.

[0120] The results are shown in Table 2.

[0121] Table 2 Corn pot test results of the products provided in the above examples and comparative examples

[0122]

[0123]

[0124] As can be seen from Table 2, after measurement and analysis, the plant height of the plant treated with the phosphate-solubilizing bacteria agent of the present application increased by more than 10.14% compared with the blank control, indicating that the phosphate-solubilizing bacteria agent has a certain growth-promoting effect. The root surface area of ​​corn treated with the phosphate-solubilizing bacteria agent increased by more than 44.23% compared with the blank control; the total phosphorus content of the plant increased by more than 7.10% compared with the blank control, indicating that the phosphate-solubilizing bacteria agent can activate phosphorus sources that are difficult for crops to absorb, while increasing the root surface area of ​​crops, expanding the range of nutrient absorption, and accelerating crop absorption.

[0125] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An Aspergillus niger, characterized in that: The Aspergillus niger ( Aspergillus niger ) is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration with the deposit number CGMCC No. 40231.

2. A culture, characterized in that The culture comprises the Aspergillus niger according to claim 1.

3. A fermentation broth, characterized in that: The fermentation broth comprises the Aspergillus niger according to claim 1.

4. A spore suspension, characterized in that The spore suspension comprises spores of Aspergillus niger according to claim 1.

5. A bacterial agent, characterized in that The bacterial agent comprises the Aspergillus niger described in claim 1.

6. A bacterial powder, characterized in that: The bacterial powder comprises the Aspergillus niger according to claim 1.

7. A composite microbial phosphate-solubilizing agent, characterized in that: The composite microbial phosphate-solubilizing agent comprises the Aspergillus niger agent prepared by using the Aspergillus niger described in claim 1 and the arbuscular mycorrhizal fungal agent prepared by using Pipepophysalis mosseae; the preservation number of Pipepophysalis mosseae is CGMCC No.40631.

8. The composite microbial phosphate-solubilizing agent according to claim 7, characterized in that: The effective viable bacteria count of the Aspergillus niger fungus agent is ≥1×10 8 pcs / g; Optionally, the spore count of the arbuscular mycorrhizal fungal agent is ≥70 / g.

9. The composite microbial phosphate-solubilizing agent according to claim 7, characterized in that: The usage amount of the Aspergillus niger agent accounts for 5-15% of the total amount of the composite microbial phosphate-solubilizing agent; the usage amount of the arbuscular mycorrhizal fungi agent accounts for 15-25% of the total amount of the composite microbial phosphate-solubilizing agent; the remainder is additives; Optionally, the additive is oyster shell powder, humic acid powder or diatomaceous earth.

10. Use of the Aspergillus niger according to claim 1, the culture according to claim 2, the fermentation liquid according to claim 3, the spore suspension according to claim 4, the bacterial agent according to claim 5, the bacterial powder according to claim 6, and the composite microbial phosphate-solubilizing bacterial agent according to any one of claims 7 to 9 for converting a phosphorus form that is difficult for crops to absorb and utilize in the soil into a phosphorus form that can be absorbed and utilized by crops.

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