Composting-promoting phosphorus-dissolving deodorizing microbial inoculum for composting, and preparation method and application of composting-promoting phosphorus-dissolving deodorizing microbial inoculum

Abstract

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

Description

Technical Field

[0001] The invention belongs to the technical field of biological agriculture and related industries, and specifically relates to a composting-promoting, phosphorus-dissolving and deodorizing bacterial agent, and a preparation method and application thereof. Background Art

[0002] Composting is the main way to effectively treat and utilize organic waste. It can convert organic waste into organic fertilizer and activate insoluble nutrients such as phosphorus into a form that can be used by crops. However, the composting process may also produce ammonia and hydrogen sulfide, causing nutrient loss and environmental pollution. Therefore, accelerating the decomposition and promoting the transformation of insoluble nutrients such as phosphorus, while reducing the emission of odorous gases such as ammonia and hydrogen sulfide, is the key to solving the problem of high-quality and clean composting.

[0003] The treatment of organic waste at home and abroad mainly includes physical, chemical and biological deodorization technologies. At present, biotechnology is often used for the clean production of organic fertilizers, and the generation of harmful gases is reduced by adding microbial agents. The existing technology focuses more on single functions such as deodorization, and there are few agents that can solve the problems of high quality and cleanliness at the same time. In addition, the few existing multifunctional agents are usually directly compounded by multiple single-function strains, and the synergistic or antagonistic effects of strains are rarely considered, resulting in better composting effects in the early stage, but limited nutrient conversion and deodorization effects in the middle and late stages. Therefore, isolating and screening efficient composting, phosphorus dissolving, and deodorizing strains, compounding them according to the interaction relationship between strains, and optimizing their compounding ratio can be an effective way to synthesize efficient and multifunctional composite bacterial agents. Summary of the invention

[0004] The first object of the present invention is to disclose a composting-promoting, phosphorus-dissolving and deodorizing bacterial agent.

[0005] The second object of the present invention is to disclose a method for preparing the above-mentioned deodorizing bacterial agent.

[0006] The third object of the present invention is to disclose the application of the above-mentioned deodorizing bacterial agent.

[0007] The objective of the present invention is achieved through the following technical solutions: A composting-promoting, phosphorus-dissolving and deodorizing bacterial agent, wherein: the bacterial agent is composed of Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1) CGMCC No.32099, Paenibacillus zeae CA ( Paenibacillus zeisoli CA) CGMCC No.32101 and Bacillus chestnut brown SC ( Bacillus badius SC) CGMCC No.32100; The mass ratios of the various strains are: Bacillus licheniformis DY ( Bacillus licheniformis DY-1): Paenibacillus zeae CA ( Paenibacillus zeisoli CA): Bacillus chestnutii SC ( Bacillus badius SC) = 1.5: (1-3): (1.5-4); the number of viable cells of each strain ≥ 5 × 10 8 CFU / g; The total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

[0008] The method for preparing the composting-promoting, phosphorus-dissolving and deodorizing bacterial agent described in the above technical solution comprises the following steps: (1) Strain activation: Bacillus licheniformis DY-1, Paenibacillus zemeri CA, and Bacillus chestnut brown SC were inoculated into LB liquid culture medium respectively, and cultured to the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Paenibacillus zemeri CA, and Bacillus chestnut brown SC; (2) Expansion culture of bacterial strains: The seed liquid of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC obtained in step (1) was transferred to a new LB liquid culture medium at an inoculation rate of 10% (v / v), and the culture was expanded to obtain the corresponding bacterial liquid; (3) Preparation of bacterial powder: The bacterial suspensions of Bacillus licheniformis DY-1, Bacillus zeae CA and Bacillus chestnut brown SC obtained in step (2) were centrifuged at 6000 rpm for 5 minutes at 4°C to collect bacterial precipitates, which were freeze-dried to prepare bacterial powders of the three strains; (4) Compounding of bacterial strains: The number of live bacteria contained in the bacterial powders of Bacillus licheniformis DY-1, Paenibacillus zemeri CA and Bacillus chestnut brown SC obtained in the detection step (3) is mixed according to the mass ratio of Bacillus licheniformis DY-1, Paenibacillus zemeri CA and Bacillus chestnut brown SC of 1.5:(1-3):(1.5-4), so as to obtain a composting-promoting, phosphorus-dissolving and deodorizing bacterial agent.

[0009] The preparation method described in the above technical solution, wherein: the culture conditions in step (1) and step (2) are: Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h; Paenibacillus zeae CA was cultured at 30°C, 180 rpm in a shaking incubator for 18-20 hours; Bacillus chestnutii SC was cultured at 30°C and 180 rpm in a shaking incubator for 16-18 hours.

[0010] The preparation method described in the above technical solution, wherein: the number of viable bacteria in the bacterial powder in step (4) is: The number of viable cells of each strain was ≥5×10 8 CFU / g; The number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

[0011] The above technical solution is used for the application of the composting-promoting, phosphorus-dissolving and deodorizing bacterial agent in the clean composting of organic waste.

[0012] Bacillus licheniformis DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly raised, single or paired, rod-shaped, and had spores.

[0013] Paenibacillus zemeri CA was screened from mushroom residue and grew on inorganic phosphorus culture medium. The colonies were white, translucent, round, slightly convex, and the bacteria were monomeric, rod-shaped, and had spores.

[0014] Bacillus chestnut brown SC was screened from livestock and poultry feces and grew on beef extract peptone agar medium. The colonies were white, opaque, round or nearly round, slightly protruding, smooth, rod-shaped, and had spores.

[0015] The present invention has the following beneficial effects: 1. The present invention screened and obtained a strain of Bacillus chestnut brown SC, which has significantly improved the effect of removing NH3 and H2S compared with the existing Bacillus.

[0016] 2. The present invention screened and obtained a strain of Bacillus licheniformis DY-1, which has excellent ability to solubilize phosphate and degrade cellulose.

[0017] 3. The present invention screened and obtained a strain of Paenibacillus zemeri CA, which has excellent phosphate-dissolving ability.

[0018] 4. The present invention is to Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Paenibacillus zeisoli CA, Bacillus chestnut SC ( Bacillus badius The results showed that the three strains of bacteria had no antagonistic effect on each other and could significantly accelerate the transformation of insoluble phosphorus, promote decomposition, and enhance the removal of NH3 and H2S. The actual test showed that the phosphorus solubilizing capacity of the three strains after compounding could reach up to 372.11 mg / L, the cellulase activity could reach up to 15.76 U / mL, and the ammonia and hydrogen sulfide removal rates could reach 83.68-84.58% and 74.67-75.35%, respectively.

[0019] Strain deposit information: 1. The present invention Bacillus licheniformisDY-1, classified as Bacillus licheniformis Bacillus licheniformis It has been deposited in the General Microbiology Center of China Microorganism Culture Collection (CGMCC). The address of the deposit unit is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.32099 and the deposit date is September 29, 2024.

[0020] 2. The present invention Bacillus badius SC, taxonomic name: Bacillus chestnut Bacillus badius It has been deposited in the General Microbiology Center of China Microorganism Culture Collection (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.32100 and the deposit date is September 29, 2024.

[0021] 3. The Paenibacillus zeisoli CA of the present invention is classified and named Paenibacillus zeisoli, and has been deposited in the General Microbiological Center (CGMCC) of China Microorganism Culture Collection Administration. The address of the depository is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 32101, and the deposit date is September 29, 2024. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is the process of strain screening and composite bacterial agent preparation;

[0023] Figure 2 is the evolutionary tree of strain Bacillus licheniformis DY-1;

[0024] Figure 3 is the evolutionary tree of strain Paenibacillus zeae CA;

[0025] Figure 4 is the evolutionary tree of strain Bacillus chestnutii SC;

[0026] Figure 5 These are the results of pairwise antagonism experiments between strains. DETAILED DESCRIPTION

[0027] To facilitate the understanding of the technical solution of the present invention, the following further describes a composting-promoting, phosphorus-dissolving and deodorizing bacterial agent and its preparation method and application in conjunction with specific embodiments.

[0028] The process of strain screening and composite bacterial agent preparation is shown in Figure 1 The specific preparation process is as described in Example 1 and Example 2.

[0029] Embodiment 1: Isolation of strains: Take 10 g of mushroom residue and cow dung compost, put them into 50 ml of sterilized water, shake them at 30°C and 180 rpm for 30 minutes, let them stand for 30 minutes, take 1 mL of the supernatant, dilute it to 10 with sterilized water, and add 10 g of the supernatant. -1 , make 10-fold gradient dilutions continuously, aspirate 0.5 mL of the diluted suspension and spread it on beef extract peptone agar medium, inorganic phosphorus medium and CMC-Na medium, and culture at 30℃ for 24-36 hours. According to the morphology and growth of the strains, select strains with different morphologies for streak culture and store at low temperature.

[0030] The culture medium and its formulation in the above embodiment are as follows: Beef extract peptone agar medium: peptone: 10 g, beef extract: 5 g, NaCl: 5 g, agar: 20 g, distilled water: 1000 mL, pH: 7.2; Inorganic phosphorus medium: glucose: 10 g, (NH4)2SO4: 0.5 g, NaCl: 0.2 g, KCl: 0.2 g, MgSO4·7H2O: 0.1 g, MnSO4·H2O: 0.002 g, FeSO4·7H2O: 0.002 g, hydroxyapatite: 5 g, bromocresol purple: 0.05 g, agar: 20 g, distilled water: 1000 mL, pH: 7.0-7.5; CMC-Na medium: CMC-Na: 15 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0031] Embodiment 2: Screening and identification of strains: 1. Screening of strains: The strains were screened by measuring the release of ammonia and hydrogen sulfide, phosphorus dissolving ability and cellulase activity. The specific steps are as follows: 1.1. Seed solution preparation: Use a sterile inoculation loop to pick a single colony of the strain that grows well on the solid culture medium from the slant, inoculate it into LB liquid culture medium, place it in a shaker at 30°C and 180 rpm, and culture it for 24 hours to prepare the seed solution.

[0032] 1.2. Screening of deamination strains: The seed solution was inoculated into 100 mL of denitrifying bacteria screening liquid medium at a 10% (v / v) inoculation rate, and the blank group was inoculated with an equal amount of sterilized seed solution medium. Each group was set up with 3 parallels. The culture was shaken for 48 hours under the same conditions as the seed solution preparation, and the remaining NH4 in the culture medium was detected by Nessler's reagent spectrophotometry. + -N, calculate NH4 + -N removal rate, screening out NH4 + -N removal rate is high.

[0033] 1.3. Screening of desulfurization strains: The seed solution was inoculated into the desulfurization bacteria screening liquid medium at a rate of 10% (v / v), and the blank group was inoculated with an equal amount of sterilized seed solution medium. Three parallels were set up for each group. The medium was shaken at 30°C and 180 rpm for 8 hours, and the remaining S in the medium was detected by methylene blue spectrophotometry. 2- , calculate S 2- Removal rate, screening out S 2- Bacteria with high removal rate.

[0034] 1.4. Screening of phosphate-dissolving strains: The seed liquid was inoculated into the inorganic phosphorus liquid medium at a 10% (v / v) inoculation rate and sterilized at 121°C for 20 minutes. Aseptic operation was performed, and the blank control was placed on a shaker at 30°C and 180 rpm for 5-7 days. The effective phosphorus content was determined using the molybdenum antimony colorimetric method.

[0035] 1.5. Screening of cellulose-degrading bacteria: The seed solution was inoculated into CMC-Na liquid culture medium at a 10% (v / v) inoculation rate, and cultured continuously at 30°C constant temperature shaker and 180 rpm for 7 days. The cellulase activity was determined by DNS method.

[0036] The culture medium and formula used in the above screening steps are as follows: Denitrifying bacteria liquid culture medium: (NH4)2SO4: 1.0 g / L, CH3COONa: 10.0 g / L, Wei's salt solution: 50mL, sterilize at 121℃ for 15 minutes.

[0037] Desulfurization bacteria liquid screening medium: NaS·9H2O: 0.8 g / L, CH3COONa: 10.0 g / L, K2HPO4: 0.5 g / L, MgCl2: 0.2 g / L, NaHCO3: 5.0 g / L, (NH4)2SO4: 0.6 g / L, sterilized at 121℃ for 15 minutes. NaS·9H2O was first dissolved in an appropriate amount of water, filtered through a 0.22 μm filter membrane in a clean bench, and mixed with other sterilized solutions.

[0038] Inorganic phosphorus medium: glucose: 10 g, (NH4)2SO4: 0.5 g, NaCl: 0.2 g, KCl: 0.2 g, MgSO4·7H2O: 0.1 g, MnSO4·H2O: 0.002 g, FeSO4·7H2O: 0.002 g, Ca3(PO4)2: 5 g, bromocresol purple: 0.05 g, distilled water: 1000 mL, pH: 7.0-7.5 CMC-Na medium: CMC-Na: 15 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0039] According to the above screening steps, three strains with strong ability to degrade cellulose, dissolve phosphorus, and remove ammonia and desulfurization were screened, numbered DY-1, CA and SC. The ability of each strain to promote decay, dissolve phosphorus and deodorize is shown in Table 1: Table 1 The ability of each strain to promote decay, dissolve phosphorus and deodorize

[0040] 2. Identification of bacterial strains: 2.1. Physiological and biochemical tests: 2.1.1. Inoculate the three strains obtained in step 1 on the culture medium, culture at 30°C for 48 hours, observe and record the colony growth and morphology, and observe and record the bacterial morphology under a microscope.

[0041] The strain DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly convex, and the bacteria were single or paired, rod-shaped, and had spores.

[0042] Strain CA was screened from mushroom residues and grew on inorganic phosphorus medium. The colonies were white, translucent, round, slightly convex, and the bacteria were monomeric, rod-shaped, and had spores.

[0043] The strain SC was screened from a cow dung pile and grew on beef extract peptone agar medium. The colonies were white, opaque, round or nearly round, slightly protruding, smooth, rod-shaped, and had spores.

[0044] 2.1.2. Physiological and biochemical tests were performed on each bacteria using the bacterial multiphase identification detection method. The specific operations are as follows: (1) Reagent strips used: API 50CH reagent strip, API 20E reagent strip (2) Supporting culture medium: API 50 CHB / E medium, API 0.85% NaCl medium (3) Preparation of bacterial suspension: Use an inoculation tool to pick an appropriate amount of colonies from the plate and place them in API 50 CHB / E medium or API 0.85% NaCl medium, grind them carefully to make a uniform bacterial suspension. (4) Prepare the training environment: Prepare a culture box and pour about 5 ml of distilled water into the honeycomb of the dish to create a wet chamber. (5) Inoculation reagent strips: Place the API 50CH reagent strip or API 20E reagent strip in the culture box and use a pipette to add the bacterial suspension into the small tube of the reagent strip. The bacterial suspension should fill the tube and cup of the CIT, VP and GEL tubes in the API 20E reagent strip. Mineral oil should be added to the ADH, LDC, ODC, H2S and URE tubes to form an anaerobic environment.

[0045] (6) Cultivation: Place the inoculated test strips in an incubator and incubate at 36°C ± 2°C for 18-24 hours.

[0046] (7) Interpretation of results: Observe the color changes of each small tube on the test strip and refer to the instructions of the test strip to determine whether it is positive, weakly positive or negative.

[0047] The physiological and biochemical experimental results of the three strains obtained are shown in Table 2.

[0048] Table 2 Physiological and biochemical characteristics of strains

[0049] Note: “+”: positive, “w”: weak positive, “-”: negative 2.2 Molecular Validation: The three strains were inoculated in LB liquid medium, cultured at 30°C in a shaking incubator for 24 hours, and centrifuged at 12000rpm to collect the bacteria. The genomes of strain DY-1, strain CA, and strain SC were extracted using the Ezup column-type bacterial genomic DNA extraction kit. PCR amplification was performed on the three strains using primers 27F and 1492R. The nucleotide sequences of the primers are as follows: 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' 1492R:5'-AGAGTTTGATCCTGGCTCAG-3' After purification, the PCR product was sent to Shanghai Biotechnology Co., Ltd. for sequencing, and the sequencing results were compared with the sequences in the NCBI website database for homology analysis. Figure 2 The evolutionary tree of strain CA is shown in Figure 3 The evolutionary tree of strain SC is shown in Figure 4 shown.

[0050] Finally, the physiological and biochemical characteristics of the three strains were combined with molecular verification to confirm that the three strains involved in the present invention belong to Bacillus licheniformis ( Bacillus licheniformis ), Paenibacillus zeisoli, Bacillus chestnut brown ( Bacillus badius ).

[0051] Embodiment 3: Compound of decomposition-promoting, phosphorus-dissolving and deodorizing bacteria agent: 1. Antagonism experiment: The three strains were tested for antagonism on solid culture medium using the filter paper method. The bacteria on the plate were the base bacteria, and the bacteria on the filter paper were the test bacteria. The growth of the base bacteria and the test bacteria was observed. If there is no inhibition zone between the two bacteria, it means there is no antagonism. Otherwise, it means there is antagonism between the strains. Figure 5 As shown, there was no antagonism between the three strains.

[0052] 2. Compounding of strains: The obtained three strains were inoculated into LB liquid medium and cultured in a shaking incubator until the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Bacillus zemeri CA and Bacillus chestnut brown SC; Bacillus licheniformis DY-1 was cultured in a shaking incubator at 30°C and 180 rpm for 14 hours; Bacillus zemeri CA was cultured in a shaking incubator at 30°C and 180 rpm for 19 hours; Bacillus chestnut brown SC was cultured in a shaking incubator at 30°C and 180 rpm for 16 hours to obtain seed solutions of the three strains; after inoculation and expansion of the culture, the seed solutions were freeze-dried (the specific method is: the seed solutions of Bacillus licheniformis DY-1, Bacillus zemeri CA and Bacillus chestnut brown SC were transferred to a new LB liquid medium at an inoculation rate of 10% (v / v); Bacillus licheniformis DY-1 was cultured in a shaking incubator at 30°C and 180 rpm for 13 hours; Bacillus zemeri CA was cultured in a shaking incubator at 30°C and 180 rpm for 1 rpm shaking incubator for 20 hours; chestnut brown Bacillus SC was cultured at 30°C, 180 rpm shaking incubator for 17 hours, and the corresponding bacterial suspensions were obtained by expansion culture; the three bacterial suspensions were centrifuged at 6000 rpm for 5 minutes at 4°C to collect the bacterial precipitates, freeze-dried, and the bacterial powders of the three strains were prepared. 8CFU / g) were compounded according to the following mass ratio: (Bacillus licheniformis DY-1: Paenibacillus zemeri CA: Bacillus chestnut brown SC) = A: (1.5:3:2); B: (1.5:4:1.5); C: (1.5:1:3); D: (1.5:1.5:4); E: (1.5:2:4); F: (1.5:1.5:3). There were 6 compound combinations in total, which were inoculated into denitrifying bacteria liquid culture medium, desulfurizing bacteria liquid culture medium, inorganic phosphorus culture medium, and CMC-Na culture medium, respectively, for the determination of cellulase activity, phosphorus solubilization ability, ammonia removal rate, and hydrogen sulfide removal rate. The results are shown in Table 3. The results in Table 3 show that the phosphate solubilizing ability of combinations A and B is higher than that of other combinations, and higher than the phosphate solubilizing ability of the strain reported in patent CN117757671A; the deodorizing ability of combinations C and D is higher than that of other combinations, and higher than the ammonia and hydrogen sulfide removal rate of the compound deodorizing bacteria agent in CN201811547950; the cellulase activity, phosphate solubilizing and deodorizing abilities of combinations E and F are all high.

[0053] Table 3 Effects of compound microbial agents on promoting decay, dissolving phosphorus and deodorizing

[0054] The above description is only a preferred embodiment of the present invention and does not impose any formal or substantial limitation on the present invention. Any technician familiar with the profession can make use of the technical contents disclosed above without departing from the scope of the technical solution of the present invention, and any slight changes, modifications and evolutions made by equivalent changes are all equivalent embodiments of the present invention; at the same time, any changes, modifications and evolutions of any equivalent changes made to the above embodiments based on the essential technology of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A composting-promoting, phosphorus-dissolving and deodorizing bacterial agent, characterized in that: The bacterial agent is composed of Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1) CGMCC No.32099, Paenibacillus zeae CA ( Paenibacillus zeisoli CA) CGMCC No.32101 and Bacillus chestnut brown SC ( Bacillus badius SC )CGMCC No.32100; The mass ratio of each strain is: Bacillus licheniformis DY-1: Bacillus zeae CA: Bacillus chestnut brown SC = 1.5: (1-3): (1.5-4); the number of viable cells of each strain is ≥ 5×10 8 CFU / g; The total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

2. A method for preparing the composting-promoting, phosphorus-dissolving and deodorizing bacterial agent according to claim 1, comprising the following steps: (1) Strain activation: Bacillus licheniformis DY-1, Paenibacillus zemeri CA, and Bacillus chestnut brown SC were inoculated into LB liquid culture medium respectively, and cultured to the logarithmic growth phase to obtain seed liquids of Bacillus licheniformis, Paenibacillus zemeri, and Bacillus chestnut brown; (2) Expansion culture of bacterial strains: The seed liquid of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC obtained in step (1) was transferred to a new LB liquid culture medium at an inoculation rate of 10% (v / v), and the culture was expanded to obtain the corresponding bacterial liquid; (3) Preparation of bacterial powder: The bacterial suspensions of Bacillus licheniformis DY-1, Bacillus zeae CA and Bacillus chestnut brown SC obtained in step (2) were centrifuged at 6000 rpm, and the bacterial precipitates were collected and freeze-dried to prepare bacterial powders of the three strains; (4) Compounding of bacterial strains: The number of live bacteria contained in the bacterial powders of Bacillus licheniformis DY-1, Paenibacillus zemeri CA and Bacillus chestnut brown SC obtained in the detection step (3) is mixed according to the mass ratio of Bacillus licheniformis DY-1, Paenibacillus zemeri CA and Bacillus chestnut brown SC of 1.5:(1-3):(1.5-4), so as to obtain a composting-promoting phosphorus-dissolving and deodorizing bacterial agent.

3. The preparation method according to claim 2, characterized in that: The culture conditions in step (1) and step (2) are: Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h; Paenibacillus zeae CA was cultured at 30°C, 180 rpm in a shaking incubator for 18-20 hours; Bacillus chestnutii SC was cultured at 30°C and 180 rpm in a shaking incubator for 16-18 hours.

4. The preparation method according to claim 2, characterized in that: The number of viable bacteria in the bacterial powder in step (4) is: The number of viable cells of each strain was ≥5×10 8 CFU / g; The number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

5. Use of the composting-promoting, phosphorus-dissolving and deodorizing bacterial agent according to claim 1 in clean composting of organic waste.

Images