Composite probiotic postbiotics capable of improving intestinal health and application of composite probiotic postbiotics

Abstract

The invention relates to a composite probiotic postbiotic capable of improving intestinal health and application of the composite probiotic postbiotic, and belongs to the technical field of microorganisms, the composite probiotic postbiotic comprises inactivated thalli and / or metabolites of a probiotic composition; the probiotic composition is prepared from the following components: the lactobacillus casei Zhang, the plant lactobacillus P8 and the bifidobacterium animalis subsp. Lactis V9. The preparation method comprises the following steps: preparing the probiotic composition from the lactobacillus casei Zhang, the plant lactobacillus P8 and the bifidobacterium animalis subsp. Lactis V9; researches show that the content of the metabolite of the complex probiotics, such as L-phenylalanyl-L-glutamic acid, glutamic acid-isoleucine, proline-histidine, L-isoleucine-L-arginine, prolyl glutamine, chrysophanol, crocin, tributyric acid and campsis grandiflora saponin C, is remarkably increased, and the content of the metabolite of the complex probiotics is remarkably increased; therefore, the composite probiotic metagen has the effects of enhancing the intestinal barrier function, regulating cellular immunity and intestinal mucosa immunity, resisting inflammation and resisting oxidation.

Description

Technical Field

[0001] The invention relates to a composite probiotic postbiotic capable of improving intestinal health and application thereof, belonging to the technical field of microorganisms. Background Art

[0002] Probiotics are living microorganisms that are beneficial to the health of the host when ingested in sufficient quantities. Sufficient quantity, live bacterial state and beneficial health functions are the core characteristics of probiotics. Human exploration of the efficacy of probiotics is a long, rigorous and scientific process. Although many probiotics claim to have various functions, there is a consensus in the academic community that the most core function so far is to improve gastrointestinal health. The composition and quantity of intestinal flora of different people vary from person to person, which may be manipulated by personal innate genetic inheritance, or may be affected by personal acquired environmental factors, dietary structure, disease conditions and drugs. The effect of probiotics on health varies from person to person. There is no "universal" probiotic strain that is suitable for everyone and all physical conditions. In the future, the industry needs to further strengthen consumer science education, popularize professional knowledge of probiotics, clarify the scientific concept of "quantity" of probiotics, etc., so that consumers can rationally choose probiotic products.

[0003] In previous clinical trials, it was found that when probiotics are used to treat some diseases, the effect may be poor or too slow. Further research shows that this is mainly because probiotics must compete for digestive adhesion sites and balance intestinal flora after entering the human digestive tract to achieve the effect of promoting health. Studies have found that direct use of the metabolic components or bacterial components of live bacteria can produce beneficial effects more quickly and better under certain conditions. Therefore, experts named the probiotics themselves, their compounds, or their fragments as postbiotics.

[0004] Studies have shown that selected postbiotics can improve immunity better than original live bacteria, and they still have a relatively high degree of biological activity even after being subjected to continuous high temperature or intestinal digestive juice treatment. In addition, some studies have also shown that postbiotics can reduce swelling, inhibit bacteria, improve intestinal barrier, resist oxidation, lower cholesterol levels, and lower blood lipids.

[0005] CN115429821A provides a composite probiotic postbiotic composition for preventing dental caries, regulating intestinal flora and immunity, as well as a preparation method and application, comprising inactivated bacterial bodies of composite probiotics and metabolites of composite probiotics; the composite probiotics include Lactobacillus casei Zhang, Bifidobacterium animalis subspecies lactis V9 and Lactobacillus plantarum P-8; the composite probiotic postbiotic composition can balance intestinal flora, protect intestinal epithelial barrier and improve immunity, as well as prevent or treat dental caries.

[0006] CN118389336A relates to an instant probiotic preparation for improving the stability of probiotics and a preparation method thereof. The preparation is a composition of Bifidobacterium animalis lactis subspecies V9, Lactobacillus plantarum P-01, Lactobacillus casei Zhang and Lactobacillus acidophilus LA-06.

[0007] CN110169983B provides a composite probiotic powder for treating irritable bowel syndrome, wherein the composite probiotic powder comprises Lactobacillus casei Zhang, Bifidobacterium animalis subspecies lactis V9 and Lactobacillus plantarum P-8.

[0008] CN116236511A, relates to a postbiotic composition and its application in the preparation of a product for inhibiting Helicobacter pylori, and provides a postbiotic composition, the components of which include inactivated bacteria and / or metabolites of a probiotic composition; the components of the probiotic composition include Lactobacillus casei Zhang, Bifidobacterium animalis subspecies lactis V9 and Lactobacillus plantarum P-8.

[0009] There is an urgent need to find more probiotic postbiotics that can improve intestinal health. Summary of the invention

[0010] To solve the above problems, the present invention provides a composite probiotic postbiotic that can improve intestinal health, wherein the composite probiotic postbiotic comprises inactivated bacteria and / or metabolites of a probiotic composition; the probiotic composition comprises Lactobacillus casei ( Lacticaseibacillus casei ) Zhang, Lactobacillus plantarum ( Lactiplantibacillus plantarum ) P8 and Bifidobacterium animalis subsp. lactis ( Bifidobacterium animalis subsp. Lactis ) V9; the preservation number of the Lactobacillus casei Zhang is CGMCC No.5469; the preservation number of the Lactobacillus plantarum P8 is CGMCC No.5468; the preservation number of the Bifidobacterium animalis subspecies lactis V9 is CGMCC No.5470.

[0011] In one embodiment of the present invention, the components of the composite probiotic postbiotics include inactivated bacteria and metabolites of the probiotic composition.

[0012] In one embodiment of the present invention, the preparation method of the composite probiotic postbiotic is: inoculating live bacteria of the probiotic composition into a fermentation medium for culturing to obtain a fermentation liquid; and post-treating the fermentation liquid to obtain the composite probiotic postbiotic.

[0013] In one embodiment of the present invention, the inoculation amount of the live bacteria of the probiotic composition in the fermentation medium is not less than 1×10 4 -1×1010 CFU / mL; preferably 1×10 5 -1×10 9 CFU / mL; preferably 1×10 5 -1×10 8 CFU / mL; 1×10 5 -1×10 7 CFU / mL; 6×10 6 CFU / mL.

[0014] In one embodiment of the present invention, in the probiotic composition, the live bacteria ratio of the strains is 1-10:1-10:1-10; preferably 1-9:1-9:1-9; preferably 1-8:1-8:1-8; preferably 1-7:1-7:1-7; preferably 1-6:1-6:1-6; preferably 1-5:1-5:1-5; preferably 1-4:1-4:1-4; preferably 1-3:1-3:1-3; preferably 1-2:1-2:1-2; most preferably 1:1:1.

[0015] In one embodiment of the present invention, the method comprises: inoculating live bacteria of a probiotic composition into a fermentation medium for culturing to obtain a fermentation broth; post-treating the fermentation broth to obtain a composite probiotic postbiotic; the components of the probiotic composition comprise Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium animalis subsp. lactis; the Lactobacillus casei has a preservation number of CGMCC No.5469; the Lactobacillus plantarum has a preservation number of CGMCC No.5468; the Bifidobacterium animalis subsp. lactis has a preservation number of CGMCC No.5470.

[0016] In one embodiment of the present invention, the method comprises: inoculating live bacteria of the probiotic composition into a fermentation medium for culturing to obtain a fermentation broth; sterilizing and inactivating the fermentation broth and then drying it to obtain a composite probiotic postbiotic; Preferably, the fermentation broth includes fermentation supernatant, crushed product, concentrate, sterilized product, gelatinized product or dried product; Preferably, the composite probiotic postbiotics include inactivated bacterial postbiotics, metabolite postbiotics or cell wall-broken bacterial postbiotics; Preferably, the composite probiotic postbiotic is a fermented liquid or a dried product; Preferably, the composite probiotic postbiotic is a metabolite postbiotic; Preferably, the composite probiotic postbiotic is a powder obtained by spray-drying or freeze-drying the fermentation broth; In one embodiment of the present invention, a composite probiotic postbiotic is used in the preparation of a product for improving intestinal health.

[0017] In one embodiment of the present invention, the amount of the postbiotic composition added to the product is 0.01-100% by mass.

[0018] In one embodiment of the present invention, a product is provided, wherein ingredients of the product include the aforementioned composite probiotic postbiotic.

[0019] The technical solution of the present invention has the following advantages: The present invention relates to a composite probiotic postbiotic that can improve intestinal health, and belongs to the field of microbial technology. The present invention provides a composite probiotic postbiotic that can improve intestinal health, wherein the components of the composite probiotic postbiotic include inactivated bacteria and / or metabolites of a probiotic composition; the components of the probiotic composition include Lactobacillus casei Zhang, Lactobacillus plantarum P8, and Bifidobacterium animalis subspecies lactis V9. Studies have shown that the composite probiotic postbiotic marker beneficial metabolites such as L-phenylalanyl-L-glutamate, glutamate-isoleucine, proline-histidine, L-isoleucine-L-arginine, prolylglutamine, chrysophanol, crocin, tributyric acid, and vinca saponin C have extremely significantly increased contents, indicating that the composite probiotic postbiotic has the effects of enhancing intestinal barrier function, regulating cellular immunity and intestinal mucosal immunity, anti-inflammatory, and anti-oxidation.

[0020] Biomaterial Deposit A strain of Lactobacillus casei ( Lacticaseibacillus casei ) Zhang, taxonomic name Lacticaseibacillus casei It was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on November 18, 2011, with the deposit number CGMCC No.5469, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0021] A strain of Lactobacillus plantarum ( Lactiplantibacillus plantarum ) P8, taxonomic name Lactiplantibacillus plantarum It was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on November 18, 2011, with the deposit number CGMCC No.5468, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0022] A strain of Bifidobacterium animalis subsp. lactis ( Bifidobacterium animalis subsp. Lactis ) V9, which was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on November 18, 2011, with the deposit number CGMCC No.5470, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 :Comparison of differences in fermentation metabolites of single strains; Figure 2 : Comparison of differences in metabolites of the two bacterial strains' combined fermentation; Figure 3 : Comparison of differences in metabolites from fermentation of three bacterial strains; Figure 4 : Comprehensive comparison chart; Figure 5 : IBS score graph; Figure 6 :Chart of changes in inflammatory factors. DETAILED DESCRIPTION

[0024] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0025] If no specific experimental steps or conditions are specified in the following examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be purchased commercially.

[0026] Example 1

[0027] This embodiment provides a probiotic postbiotic, wherein the components of the probiotic postbiotic include inactivated probiotic bacteria and metabolites, and the preparation method of the probiotic postbiotic is as follows: In terms of mass percentage, the fermentation medium consists of 1% defatted soy flour, 8% defatted milk powder and the balance water; after the components of the fermentation medium are mixed in proportion, the material is stirred at 58°C (i.e., stirred at 150rpm) for 15 minutes to obtain a liquid; the liquid is homogenized once at 58°C, at a primary pressure of 15Mpa and a secondary pressure of 5.0Mpa to obtain a homogenized liquid; the homogenized liquid is sterilized at 93°C for 30 minutes to obtain a sterilized liquid; the sterilized liquid is cooled to 35°C to obtain a fermentation medium. The fermentation strains are inoculated respectively, and the fermentation is terminated when the pH value is 4.50, and a powdered composite probiotic postbiotic is obtained by a spray drying process (inlet air temperature 170°C, outlet air temperature 70°C).

[0028] Table 1 Strain names and sources .

[0029] Example 2

[0030] This embodiment provides a composite probiotic postbiotic, wherein the components of the composite probiotic postbiotic include inactivated probiotic bacteria and metabolites; the preparation method of the composite probiotic postbiotic is as follows: The fermentation steps were the same as those in Example 1; wherein the target strains are shown in Table 2.

[0031] Table 2 Combination of two bacterial strains .

[0032] Example 3

[0033] This embodiment provides a composite probiotic postbiotic, wherein the components of the composite probiotic postbiotic include inactivated probiotic bacteria and metabolites; the preparation method of the composite probiotic postbiotic is as follows: The fermentation steps were the same as those in Example 1; wherein the target strains are shown in Table 3.

[0034] Table 3 Combination of three bacterial strains .

[0035] Example 4

[0036] This embodiment provides a composite probiotic postbiotic, wherein the components of the composite probiotic postbiotic include inactivated probiotic bacteria and metabolites; the preparation method of the composite probiotic postbiotic is as follows: The fermentation steps were the same as those in Example 1; wherein the target strains are shown in Table 4.

[0037] Table 4 Combination of four bacterial strains .

[0038] Experimental Example 1: Differences in metabolites of probiotics produced by fermentation of single strains

[0039] 1. Test methods 1.1 Bacterial count detection method The postbiotic samples were reconstituted with sterile PBS, centrifuged at 5000×g, 4°C for 5 min, resuspended, the precipitate discarded, and the samples were serially diluted to 10 5 The reconstituted sample was then stained with a 10 μM cFDA molecular probe, incubated at 37°C in the dark for 20 min, taken out and stained with a final concentration of 5 μM PI dye, shaken for 1 min, incubated in a dark room for 15 min, and the fluorescence intensity was detected by flow cytometry to characterize the bacterial count of the postbiotic.

[0040] 1.2 Metabolite detection methods The probiotic postbiotic samples described in Example 1 were commissioned to Maiwei Metabolism Company for metabolite detection. The raw data were converted into mzXML format using ProteoWizar d software, and then processed using R language (using XCMS kernel), such as peak identification, extraction, alignment and integration. Subsequently, the data were compared with the self-built BiotreeDB secondary mass spectrometry database (version 2.1) for substance annotation, and the algorithm score threshold was set to 0.3.

[0041] The contents of signature beneficial metabolites including L-phenylalanyl-L-glutamate, glutamate-isoleucine, proline-histidine, L-isoleucine-L-arginine, prolylglutamine, chrysophanol, crocin, tributyric acid and genistein C were selected for analysis.

[0042] 2. Experimental results The number of postbiotic cells is shown in Table 5. The results show that the number of postbiotic cells after fermentation by LCZ, V9 and P8 is relatively high, all exceeding 80 billion cells / g.

[0043] Table 5 Number of postbiotic cells after fermentation of a single strain (×10 10 Cells / g) .

[0044] The relative content of metabolites is shown in Figure 1 The contents of the probiotic postbiotic signature metabolites obtained by fermentation of LCZ, P8 and V9 were significantly higher than those of other strains, indicating that the probiotic postbiotics prepared by fermentation of Lactobacillus casei Zhang, Lactobacillus plantarum P8 and Bifidobacterium animalis subspecies lactis V9 have greater potential in improving intestinal health, enhancing intestinal barrier, anti-inflammatory and antioxidant functions.

[0045] Experimental Example 2: Differences in metabolites of postbiotics produced by fermentation of two bacterial strains to prepare composite probiotics

[0046] Experimental methods The same as Experimental Example 1, except that the composite probiotic postbiotics used are those prepared in Example 2.

[0047] Experimental Results The number of postbiotic cells is shown in Table 2. The results show that the number of postbiotic cells after fermentation by LCZ+V9, LCZ+P8, V9+P8 and V9+NM-94 is relatively high, all exceeding 90 billion cells / g.

[0048] Table 6 The number of postbiotic cells after fermentation of the two bacterial strains (×10 10 Cells / g) .

[0049] like Figure 2 , the content of 9 kinds of marked beneficial metabolites of compound probiotic postbiotics obtained by fermentation of 21 groups of two strains of bacteria was determined, and it was found that the content of beneficial metabolites of the combination of LCZ+V9, LCZ+P8, V9+P8 and V9+NM-94 was significantly higher than that of other combinations, and the content was higher than that of probiotic postbiotics obtained by fermentation of single strains. It is speculated that the effect of compound probiotic products is better than that of single strain probiotic products, increasing the content of marked beneficial metabolites in compound probiotic postbiotics and enhancing the effect of improving intestinal health.

[0050] Experimental Example 3: Differences in metabolites of postbiotics produced by fermentation of three bacterial strains to prepare composite probiotics

[0051] 1. Experimental Methods The same as Experimental Example 1, except that the composite probiotic postbiotics used are those prepared in Example 3.

[0052] 2. Experimental results The number of postbiotic cells is shown in Table 3. The results show that the number of postbiotic cells after fermentation by LCZ, V9 and P8 is relatively high, exceeding 130 billion cells / g.

[0053] Table 7 Number of postbiotic cells after fermentation of three-strain composite strains (×10 10 Cells / g) .

[0054] Depend on Figure 3 It can be seen that after increasing the number of fermentation strains, only the content of the characteristic metabolites of the combination of LCZ+V9+P8 has been significantly increased, and there is no obvious regularity in the combination of LCZ, P8 and V9 with other strains, and at the same time, the content has not been significantly improved compared with Experimental Example 2. This shows that the composite probiotic postbiotic characteristic beneficial metabolites prepared by the combination of LCZ+V9+P8 fermentation have the highest content, and have better functions such as improving intestinal health, strengthening intestinal barrier, anti-inflammatory and antioxidant.

[0055] Experimental Example 4: Differences in metabolites of postbiotics produced by fermentation of four bacterial strains to prepare composite probiotics

[0056] 1. Experimental Methods The same as Experimental Example 1, except that the composite probiotic postbiotics used are those prepared in Example 4.

[0057] 2. Experimental results Table 8 Number of postbiotic bacteria after fermentation of four bacterial strains (×10 11 Cells / g) .

[0058] The measurement results of this experimental example were compared with the measurement of the content of characteristic beneficial metabolites of the LCZ+V9+P8 combination in Experimental Example 3, the LCZ+V9, LCZ+P8, V9+P8 combinations in Experimental Example 2, and the LCZ, P8 and V9 in Experimental Example 1. It was found that the characteristic beneficial metabolic content of the composite probiotic postbiotics prepared by fermentation of the LCZ+V9+P8 combination in Experimental Example 3 was significantly higher than the measurement results of this experimental example, indicating that there is no correlation between the number of fermentation strains and the content of beneficial metabolites, and increasing the number of fermentation strains cannot increase the content of beneficial metabolites.

[0059] The content of the characteristic beneficial metabolism of the composite probiotic postbiotic prepared by the combined fermentation of LCZ+V9+P8 in Experimental Example 3 was significantly higher than the measurement results of the other two groups, indicating that the combination of Lactobacillus casei Zhang, Lactobacillus plantarum P8, and Bifidobacterium animalis subspecies lactis V9 was the optimal combination, and the combined fermentation had a synergistic effect between strains, which could increase the content of beneficial metabolites in the composite probiotic postbiotic, improve intestinal health, and increase the intestinal barrier function. The results are shown in Figure 4 .

[0060] In summary, the composite probiotic postbiotics prepared by fermentation of Lactobacillus casei Zhang, Lactobacillus plantarum P8, and Bifidobacterium animalis subspecies lactis V9 in a 1:1:1 ratio have the highest contents of the signature beneficial metabolites L-phenylalanyl-L-glutamate, glutamate-isoleucine, proline-histidine, L-isoleucine-L-arginine, prolylglutamine, chrysophanol, crocin, tributyric acid, and scutellaria baicalensis saponin C, and have good effects of enhancing intestinal barrier function, regulating cellular immunity and intestinal mucosal immunity, and anti-inflammatory and antioxidant effects.

[0061] Example 5: Effect of Alleviating Irritable Bowel Syndrome

[0062] 1. Research subjects 76 IBS patients who were treated in the outpatient and inpatient departments of the Department of Gastroenterology of a certain hospital and met the Rome III diagnostic criteria were selected as the research subjects, aged 18-80 years old, regardless of gender. All patients had completed routine blood, urine and stool tests, liver and kidney function, abdominal ultrasound, chest X-ray, gastrointestinal endoscopy, and capsule endoscopy, and no obvious organic changes were found. Exclusion criteria: patients with other gastrointestinal lesions, heart, liver and kidney dysfunction, malignant tumors, diabetes, previous abdominal surgery, pregnant or lactating women, those who had taken drugs that affected the observation of this trial (antibiotics, traditional Chinese medicine, etc.) within 4 weeks before the trial, autoimmune diseases, and those who could not be re-examined and followed up on schedule.

[0063] 2. Experimental methods A total of 80 IBS patients were divided into four groups (Post1, Post2, Post3 and Pro), with 20 patients in each group. While receiving basic treatment, the patients were treated with a preparation containing a compound probiotic postbiotic (10 billion cells / g, 30 billion cells / g and 50 billion cells / g, with maltodextrin as carrier) and a preparation containing a compound probiotic (the number of viable LCZ bacteria was ≥3.0×10 9 CFU / g, V9 viable bacteria count ≥4.0×10 9 CFU / g, P8≥3.0×10 9 CFU / g, maltodextrin as carrier) preparation was used to compare the efficacy in people with irritable bowel, and the treatment time was 15 days.

[0064] 3. Observation indicators (1) Stool quality scoring: According to the Bristol scoring standard, stool is divided into seven types. Type 1: hard balls (difficult to pass); Type 2: sausage-shaped, but with uneven surface; Type 3: sausage-shaped, but with cracks on the surface; Type 4: like a sausage or snake, with a smooth surface; Type 5: soft blocks with smooth broken edges (easy to pass); Type 6: fluffy blocks with rough edges, mushy stool; Type 7: watery, no solid blocks (completely liquid); Types 1 and 2 indicate constipation, scored 2 and 1 points respectively; Types 3 and 4 are ideal stool shapes, scored 0 points; Types 5 to 7 indicate possible diarrhea, scored 1 point, 2 points, and 3 points respectively.

[0065] (2) IBS-related symptom scores: Before and during treatment, record the following points every day: duration of abdominal pain, duration of abdominal distension, presence or absence of abdominal distension and pain during defecation, proportion of abnormal bowel movement frequency, proportion of abnormal bowel movement characteristics, proportion of mucus stool and proportion of tenesmus. Calculate the total score of each symptom according to the scoring criteria in Table 9 and count it once a week.

[0066] Table 9 IBS-related symptom score table (points) .

[0067] Efficacy judgment criteria: According to the patient's total symptom score and stool characteristic score, cured: all symptoms completely disappeared; markedly effective: all symptoms were significantly alleviated (the total symptom score after taking the medicine decreased by >50% compared with before taking the medicine), and the stool characteristic score decreased by ≥2 levels; effective: all symptoms were slightly alleviated (the total symptom score after taking the medicine decreased by 20% to 50% compared with before taking the medicine), and the stool characteristic score decreased by 1 level; ineffective: no significant alleviation of all symptoms (the total symptom score after taking the medicine decreased by 20% compared with before taking the medicine), and the stool characteristic score grade did not decrease.

[0068] 4. Observation method (1) Compare the IBS symptom scores and cure rates of patients in each group at 0, 3, 6, 9, 12, and 15 days of treatment; (2) compare the levels of IL-6, IL-8, LPS, and TNF-α in the blood of patients on days 0 and 15 of treatment; 5. Treatment results Table 10 The therapeutic effects and cure rates of compound probiotics, postbiotics and compound probiotics on IBS patients .

[0069] The therapeutic effect and cure rate of compound probiotic postbiotics on IBS patients are shown in Table 10, from which it can be seen that the compound probiotic lactic acid bacteria powder has a very good therapeutic effect on irritable bowel syndrome. The effective rate of Post1 group reached 100% at 15 days, the effective rate of Post2 group reached 100% at 12 days, and the effective rate of Post3 group reached 100% at 9 days. It has the characteristics of fast effect and no recurrence.

[0070] Effects of compound probiotics and postbiotics on IBS symptom scores in IBS patients Figure 5 As shown, it can be seen that the IBS symptom scores of patients in the Post3 group began to decrease significantly after 6 days of treatment, that is, the IBS symptom scores of patients were significantly improved after 6 days of treatment with compound probiotics and postbiotics. At the same time, starting from the 9th day, the treatment effects of the three groups of compound probiotics and postbiotics were significantly better than those of the probiotics group. The above results show that compound probiotics and postbiotics treatment has a very good effect on irritable bowel syndrome.

[0071] Depend on Figure 6 It can be seen that after taking the compound probiotics and postbiotics for 15 days, the levels of IL-6, IL-8, LPS and TNF-α in the patient's blood indicators were significantly lower than before treatment. The above results show that the compound probiotics and postbiotics can reduce the levels of inflammatory factors in the patient's blood.

[0072] In summary, the composite probiotic postbiotic prepared by fermenting Lactobacillus casei Zhang, Lactobacillus plantarum P8, and Bifidobacterium animalis subspecies lactis V9 in a ratio of 1:1:1 has a significant therapeutic effect on irritable bowel syndrome. Therefore, the composite probiotic postbiotic that can improve intestinal health provided by the present invention has the characteristics of being able to effectively treat irritable bowel syndrome (IBS), improve symptoms, and reduce inflammatory responses.

[0073] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A composite probiotic postbiotic, characterized in that: The components of the composite probiotic postbiotics include inactivated bacteria and / or metabolites of the probiotic composition; the components of the probiotic composition include Lactobacillus casei ( Lactobacillus casei ) Zhang, Lactobacillus plantarum ( Lactobacillus plantarum ) P8 and Bifidobacterium animalis subsp. lactis ( Bifidobacterium animalis subsp. Lactis ) V9; the preservation number of the Lactobacillus casei is CGMCC No.5469; the preservation number of the Lactobacillus plantarum is CGMCC No.5468; the preservation number of the Bifidobacterium animalis subspecies lactis is CGMCC No.5470.

2. The composite probiotic postbiotic according to claim 1, characterized in that The inoculation amount of the live bacteria of the probiotic composition in the fermentation medium is not less than 6×10 4 -CFU / mL-6×10 8 CFU / mL.

3. The composite probiotic postbiotic according to claim 1 or 2, characterized in that: In the probiotic composition, the ratio of live bacteria of the strains is 1-10:1-10:1-10.

4. A method for preparing the composite probiotic postbiotic according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: inoculating live bacteria of the probiotic composition into a fermentation medium for culturing to obtain a fermentation liquid; and post-treating the fermentation liquid to obtain a composite probiotic postbiotic.

5. Use of the composite probiotic postbiotic according to any one of claims 1 to 3 in the preparation of a product for improving intestinal health.

6. A product, characterized in that The ingredients of the product include the composite probiotic postbiotics according to claim 1.

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