Method for rapidly obtaining genetic transformation root system of citrus rootstock and application of method
By creating wounds on the stems of Citrus plant seedlings and using Agrobacter rhizobium K599 and high-pressure propagation box, the problem of time-consuming and susceptible to contamination in traditional citrus is solved, and rapid and efficient genetic transformation is achieved, reducing research costs.
CN119709824APending Publication Date: 2025-03-28GANNAN NORMAL UNIV
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Patent Information
- Application Number
- CN202411458568.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-18
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
Traditional citrus genetic transformation methods take a long time and are easily contaminated. The transformation cycle is between 2 and 3 years, which increases the research cost and time.
Method used
By creating wounds on the stems of citrus plant seedlings, covering the hygroscopic material that is infiltrated with Agrobacterium rhizosus K599, fixing the high-pressure breeding box, promoting root growth at the wound, and finally transplanting and obtaining genetic transformation plants.
Benefits of technology
This method effectively shortens the genetic transformation cycle, reduces costs, improves efficiency, avoids the generation of chimera, and can be reused.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the technical field of plant genetic transformation, and particularly relates to a method for rapidly obtaining a citrus rootstock genetic transformation root system and application thereof. According to the genetic transformation method, agrobacterium rhizogenes K599 is used as a medium, an infection solution is transfected through a wound of a citrus plant, the infection solution can be quickly transfected into a stem segment of the citrus plant, and finally, the genetically transformed citrus plant can be quickly obtained in a high-pressure propagation mode. The method provided by the invention can effectively shorten the transformation system period, reduce the genetic transformation cost, improve the genetic transformation efficiency and avoid the generation of chimeras, and the materials can be repeatedly utilized.
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