A quality detection device for mesenchymal stem cells

Abstract

The present invention discloses a quality detection device for mesenchymal stem cells in the field of cell detection technology. The device comprises a support and two culture dishes, which are symmetrically distributed on the left and right sides of the support. A circulation mechanism is provided on the support, which is used to connect the two culture dishes so that the tissue fluid in the two culture dishes can circulate with each other. A docking mechanism is symmetrically provided on the left and right sides of the support, which is used to seal and dock the culture dishes with the circulation mechanism. The device circulates the tissue fluid in the culture dish containing the mesenchymal stem cell tissue fluid into another empty culture dish through the circulation mechanism. During the circulation process, each mesenchymal stem cell can be detected in real time by the detection mechanism. Since the mesenchymal stem cell tissue fluid continuously flows through the monitoring point, the cultivation of the mesenchymal stem cells can be effectively prevented from being affected by long-term light exposure.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and in particular to a quality detection device for mesenchymal stem cells. Background Art

[0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cell that possess all the common properties of stem cells, namely self-renewal and multidirectional differentiation. They are also widely used in clinical practice. Their combination with hematopoietic stem cells can improve transplant success rates and accelerate hematopoietic reconstitution.

[0003] Since mesenchymal stem cells require light exposure during observation and inspection, and since the population distribution of mesenchymal stem cells per milliliter in the cell tissue fluid is relatively small, a longer period of cultivation is required for testing. During testing, long-term light exposure will affect the cultivation of mesenchymal stem cells, and thus affect the test results. Summary of the Invention

[0004] The purpose of the present invention is to provide a quality detection device for mesenchymal stem cells to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a mesenchymal stem cell quality detection device, comprising a scaffold and two culture dishes, wherein the two culture dishes are symmetrically distributed on the left and right sides of the scaffold;

[0006] The support is provided with a circulation mechanism, which is used to connect the two culture dishes so that the tissue fluids in the two culture dishes can flow between each other;

[0007] The bracket is symmetrically provided with docking mechanisms on the left and right sides, and the docking mechanisms are used to seal and dock the culture dish with the circulation mechanism;

[0008] The support is provided with a detection mechanism, which is used to detect the quality of mesenchymal stem cells in the tissue fluid at a position inside the support by observing the tissue fluid flowing to the position inside the support when the tissue fluid in the culture dish circulates in the circulation mechanism.

[0009] Preferably, the circulation mechanism includes a support plate, which is fixedly connected to the bracket and is located in the middle of the bracket; an observation plate is fixedly connected above the support plate, and circulation tubes are rotatably connected to the front and rear sides of the observation plate, and the circulation tubes are rotatably connected to the bracket; the right end of the front circulation tube and the left end of the rear circulation tube are rotatably connected to connecting tubes, and the connecting tubes on the left and right sides are respectively located above the culture dishes on the left and right sides.

[0010] Preferably, the observation plate has a flat inner cavity and the front and rear flow tubes are both connected to the inner cavity of the observation plate.

[0011] Preferably, the docking mechanism includes a docking tube, which is located directly above the culture dish and is fixedly connected to the connecting tube; a sealing ring is fixedly connected to the bottom of the docking tube, and the upper end of the culture dish fits with the sealing ring; a fastening mechanism is provided on the docking tube, which is used to fix the culture dish and apply upward pressing force to it.

[0012] Preferably, the fastening mechanism includes a sliding ring, which is sleeved on the docking tube; the surface of the docking tube is provided with a thread and a plurality of vertical grooves are opened on the surface of the docking tube; the sliding ring is clearance-matched with the docking tube and is slidably connected to the vertical grooves on the surface of the docking tube; four connecting blocks are fixedly connected to the sliding ring, a friction block is provided on the inner side of the connecting block, two connecting rods are provided between the friction block and the connecting block, and the two ends of the two connecting rods are rotatably connected to the friction block and the connecting block respectively; a driving mechanism is provided on the sliding ring, and the driving mechanism is used to drive the friction block to fit against the side wall of the culture dish and drive the sliding ring to move upward after the friction block fits against the side wall of the culture dish.

[0013] Preferably, the thread on the docking sleeve has self-locking properties; the inner part of the friction block is made of rubber; and the two connecting rods are of equal length and parallel to each other.

[0014] Preferably, the driving mechanism includes a first rotating ring, which is sleeved on the docking tube and is threadedly connected to the docking tube; the first rotating ring is located above the sliding ring and is rotatably connected to the sliding ring; a sliding block is slidably connected to the connecting block, and a sliding rod is provided on the sliding block, and the sliding rod is slidably connected to the sliding block and one inner end of the sliding rod is fixedly connected to the friction block; a spring is sleeved on the outer side of the sliding rod, one end of the spring is fixedly connected to the sliding rod and the other end is fixedly connected to the sliding block; an extrusion mechanism is provided on the first rotating ring, and the extrusion mechanism is used to squeeze the sliding rod to the outer position when the first rotating ring rotates, and to cancel the extrusion of the sliding rod when the first rotating ring rotates in the other direction.

[0015] Preferably, the extrusion mechanism includes four arc-shaped frames, which are fixedly connected to four connecting blocks respectively; a second rotating ring is provided at the bottom position of the first rotating ring, the second rotating ring is slidingly connected to the four arc-shaped frames and the second rotating ring is fixedly connected to the first rotating ring; four first extrusion blocks are fixedly connected to the second rotating ring at positions close to the four sliding rods; a second extrusion block is fixedly connected to one end of the outer side of the sliding rod, and the second extrusion block is located on the moving track of the first extrusion block.

[0016] Preferably, the detection mechanism includes an observation camera, which is located above the observation plate and fixedly mounted on a bracket; the observation plate is made of transparent glass and a fill light is provided below the observation plate, and the fill light is fixedly connected to the support plate; a display screen is fixedly connected above the bracket, and the display screen is used to display the image observed by the observation camera in real time; a diversion mechanism is provided on the bracket, and the diversion mechanism is used to flow tissue fluid in one culture dish into another culture dish.

[0017] Preferably, the flow guide mechanism includes two cylinders, which are respectively located at the front and rear sides of the bracket and are both rotatably connected to the bracket; the bottom end of the front cylinder and the bottom end of the rear cylinder are respectively rotatably connected to the flow pipes on the front and rear sides; bevel gears are symmetrically rotatably connected to the left and right sides of the flow pipe, the two bevel gears are fixedly connected to each other and the surfaces of the two bevel gears are meshed with bevel gear rings, and the two bevel gear rings are respectively fixedly connected to the connecting pipe and the bracket.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] When detecting mesenchymal stem cells, the present invention circulates the tissue fluid in a culture dish containing mesenchymal stem cell tissue fluid into another empty culture dish through a circulation mechanism. During the circulation process, each mesenchymal stem cell can be detected in real time by the detection mechanism. Since the mesenchymal stem cell tissue fluid continuously flows through the monitoring point, the influence of long-term light exposure on the cultivation of mesenchymal stem cells can be effectively avoided.

[0020] The present invention turns the docking tube over and then puts the culture dish upside down on the docking tube. By rotating the second rotating ring, the four friction blocks can fit with the culture dish. As the second rotating ring is continuously rotated, the four friction blocks can clamp the culture dish and drive the culture dish to fit tightly with the sealing ring. The installation and disassembly are relatively convenient and the sealing performance is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the split structure of the present invention;

[0023] Figure 3 Schematic diagram of the structure of the docking mechanism in the present invention;

[0024] Figure 4 Schematic diagram of the split structure of the docking mechanism in the present invention;

[0025] Figure 5 Schematic diagram of the structure of the friction block in the present invention;

[0026] Figure 6 Schematic diagram of the structure of the second rotating ring in the present invention;

[0027] Figure 7 It is a structural schematic diagram of the detection mechanism in the present invention.

[0028] The reference numerals are as follows:

[0029] 1. Bracket; 2. Culture dish; 3. Support plate; 4. Observation plate; 5. Circulation tube; 6. Connecting tube; 7. Docking tube; 8. Sealing ring; 9. Sliding ring; 10. Connecting block; 11. Friction block; 12. Connecting rod; 13. First rotating ring; 14. Sliding block; 15. Sliding rod; 16. Spring; 17. Arc frame; 18. Second rotating ring; 19. First extrusion block; 20. Second extrusion block; 21. Observation camera; 22. Fill light; 23. Display screen; 24. Cylinder; 25. Bevel gear; 26. Bevel gear ring. DETAILED DESCRIPTION

[0030] See also Figure 1-Figure 7 The present invention provides a technical solution: a quality detection device for mesenchymal stem cells, comprising a scaffold 1 and two culture dishes 2, wherein the two culture dishes 2 are symmetrically distributed on the left and right sides of the scaffold 1;

[0031] The support 1 is provided with a circulation mechanism, which is used to connect the two culture dishes 2 so that the tissue fluids in the two culture dishes 2 can flow between each other;

[0032] The bracket 1 is symmetrically provided with docking mechanisms on the left and right sides, and the docking mechanisms are used to seal and dock the culture dish 2 with the circulation mechanism;

[0033] The support 1 is provided with a detection mechanism, which is used to detect the quality of mesenchymal stem cells in the tissue fluid at a position inside the support 1 by observing the tissue fluid flowing to the position inside the support 1 when the tissue fluid in the culture dish 2 circulates in the circulation mechanism;

[0034] During operation, mesenchymal stem cell tissue fluid is placed in one of the culture dishes 2 for culture, and the culture dish 2 cultured with mesenchymal stem cells and another empty culture dish 2 are docked with the circulation mechanism on the bracket 1 through the docking mechanism; when the mesenchymal stem cells need to be detected, the tissue fluid in the culture dish 2 cultured with mesenchymal stem cell tissue fluid can be circulated to the other empty culture dish 2 through the circulation mechanism. During the circulation process, each mesenchymal stem cell can be detected in real time through the detection mechanism. Since the mesenchymal stem cell tissue fluid continuously flows through the monitoring point, it can effectively avoid the influence of long-term light exposure on the cultivation of mesenchymal stem cells.

[0035] like Figure 7As shown, as a further embodiment of the present invention, the circulation mechanism includes a support plate 3, which is fixedly connected to the bracket 1 and is located in the middle of the bracket 1; an observation plate 4 is fixedly connected above the support plate 3, and circulation tubes 5 are rotatably connected to the front and rear sides of the observation plate 4, and the circulation tubes 5 are rotatably connected to the bracket 1; the right end of the front circulation tube 5 and the left end of the rear circulation tube 5 are rotatably connected to the connecting tube 6, and the left and right connecting tubes 6 are respectively located above the left and right culture dishes 2;

[0036] The observation plate 4 has a flat inner cavity and the front and rear flow tubes 5 are both connected to the inner cavity of the observation plate 4;

[0037] During operation, when both culture dishes 2 are docked with the circulation mechanism, the tissue fluid in one of the culture dishes 2 can flow into the observation plate 4 through the corresponding connecting tube 6 and the circulation tube 5. Since the inner cavity of the observation plate 4 is relatively flat, the distribution area of ​​the tissue fluid can be increased, thereby effectively improving the observation effect of the tissue fluid; when the tissue fluid flows into the observation plate 4, the tissue fluid passing through here can be observed through the observation plate 4; the tissue fluid in the observation plate 4 finally flows into the other culture dish 2 through the other circulation tube 5 and the other connecting tube 6 to continue culturing.

[0038] like Figure 3-6 As shown, as a further embodiment of the present invention, the docking mechanism includes a docking tube 7, which is located directly above the culture dish 2 and is fixedly connected to the connecting tube 6; a sealing ring 8 is fixedly connected to the bottom of the docking tube 7, and the upper end of the culture dish 2 is in contact with the sealing ring 8; a fastening mechanism is provided on the docking tube 7, which is used to fix the culture dish 2 and apply an upward pressing force thereto;

[0039] The fastening mechanism includes a sliding ring 9, which is sleeved on the docking tube 7; the surface of the docking tube 7 is provided with a thread and a plurality of vertical slide grooves are opened on the surface of the docking tube 7; the sliding ring 9 is clearance-matched with the docking tube 7 and is slidably connected to the vertical slide grooves on the surface of the docking tube 7; four connecting blocks 10 are fixedly connected to the sliding ring 9, and a friction block 11 is provided on the inner side of the connecting block 10. Two connecting rods 12 are provided between the friction block 11 and the connecting block 10, and the two ends of the two connecting rods 12 are rotatably connected to the friction block 11 and the connecting block 10 respectively; a driving mechanism is provided on the sliding ring 9, which is used to drive the friction block 11 to fit the side wall of the culture dish 2 and drive the sliding ring 9 to move upward after the friction block 11 fits the side wall of the culture dish 2;

[0040] The thread on the docking sleeve 7 is self-locking; the inner part of the friction block 11 is made of rubber; the two connecting rods 12 are equal in length and parallel to each other;

[0041] The driving mechanism includes a first rotating ring 13, which is sleeved on the docking tube 7 and is threadedly connected to the docking tube 7; the first rotating ring 13 is located above the sliding ring 9 and is rotatably connected to the sliding ring 9; a sliding block 14 is slidably connected to the connecting block 10, and a sliding rod 15 is provided on the sliding block 14, the sliding rod 15 is slidably connected to the sliding block 14, and one inner end of the sliding rod 15 is fixedly connected to the friction block 11; a spring 16 is sleeved on the outer side of the sliding rod 15, one end of the spring 16 is fixedly connected to the sliding rod 15 and the other end is fixedly connected to the sliding block 14; an extrusion mechanism is provided on the first rotating ring 13, which is used to squeeze the sliding rod 15 to the outer position when the first rotating ring 13 rotates, and cancel the extrusion of the sliding rod 15 when the first rotating ring 13 rotates in the other direction;

[0042] The extrusion mechanism includes four arc-shaped frames 17, which are respectively fixedly connected to the four connecting blocks 10; a second rotating ring 18 is provided at the bottom of the first rotating ring 13, which is slidably connected to the four arc-shaped frames 17 and fixedly connected to the first rotating ring 13; four first extrusion blocks 19 are respectively fixedly connected to the second rotating ring 18 at positions near the four sliding rods 15; a second extrusion block 20 is fixedly connected to the outer end of the sliding rod 15, and the second extrusion block 20 is located on the movement track of the first extrusion block 19;

[0043] During operation, when the culture dish 2 needs to be installed, the docking tube 7 is turned over first, and then a clean culture dish 2 is placed upside down on the docking tube 7. Then, the second rotating ring 18 is manually driven to rotate. When the second rotating ring 18 rotates, it drives the four first extrusion blocks 19 connected thereto to move. When the first extrusion blocks 19 move, they will slowly separate from the second extrusion blocks 20. When the first extrusion blocks 19 are separated from the second extrusion blocks 20, under the action of the spring 16, the friction block 11 will directly stick to the surface of the culture dish 2;

[0044] As the second rotating ring 18 is driven to rotate, the second rotating ring 18 drives the first rotating ring 13 to rotate. When the first rotating ring 13 rotates, it starts to move in the direction close to and away from the culture dish 2 under the action of the thread on the surface of the docking tube 7; when the first rotating ring 13 moves, it drives the sliding ring 9 to move, and the sliding ring 9 drives the friction block 11 to move through the two connecting rods 12. At this time, the friction block 11 is subjected to forces inward and in the direction close to the docking tube 7 under the action of the two connecting rods 12. The force inward makes the friction block 11 fit more closely with the culture dish 2, thereby increasing the friction between the friction block 11 and the culture dish 2; the force in the direction close to the docking tube 7 drives the culture dish 2 to fit more closely with the sealing ring 8, thereby achieving the effect of sealing and installing the culture dish 2;

[0045] After the culture dish 2 is installed, it is turned over to the front, and then the other docking tube 7 is turned over and tissue fluid is injected into it. The tissue fluid will eventually flow into the culture dish 2 for cultivation; after the tissue fluid is injected, another culture dish 2 can be installed in the same way as the above operation.

[0046] like Figure 7 As shown, as a further embodiment of the present invention, the detection mechanism includes an observation camera 21, which is located above the observation plate 4 and is fixedly mounted on the bracket 1; the observation plate 4 is made of transparent glass and a fill light 22 is provided below the observation plate 4, which is fixedly connected to the support plate 3; a display screen 23 is fixedly connected above the bracket 1, and the display screen 23 is used to display the image observed by the observation camera 21 in real time; a diversion mechanism is provided on the bracket 1, and the diversion mechanism is used to flow the tissue fluid in one culture dish 2 into another culture dish 2;

[0047] The flow guide mechanism includes two cylinders 24, which are respectively located at the front and rear sides of the bracket 1 and are both rotatably connected to the bracket 1; the bottom ends of the front cylinder 24 and the bottom ends of the rear cylinder 24 are respectively rotatably connected to the flow pipes 5 on the front and rear sides; bevel gears 25 are symmetrically rotatably connected to the left and right sides of the flow pipe 5, and the two bevel gears 25 are fixedly connected to each other and meshed with bevel gear rings 26 on the surfaces of the two bevel gears 25. The two bevel gear rings 26 are respectively fixedly connected to the connecting pipe 6 and the bracket 1;

[0048] During operation, when it is necessary to detect mesenchymal stem cells in the tissue fluid, the two flow tubes 5 are driven to rotate respectively by starting the two cylinders 24, and the flow tube 5 on the side with tissue fluid rotates upward and the flow tube 5 on the other side rotates downward; when the flow tube 5 rotates upward, it drives the culture dish 2 with tissue fluid to move upward; when the flow tube 5 rotates, since the bevel gear ring 26 connected to the bracket 1 remains stationary, the bevel gear 25 engaged with it starts to rotate, and when the bevel gear 25 rotates, it drives the bevel gear 25 on the other side to rotate, and the bevel gear 25 on the other side drives the connecting tube 6 to rotate through the bevel gear ring 26 engaged with it; when the flow tube 5 rotates upward by a certain angle, the corresponding culture dish 2 is in an inverted state, and the tissue fluid in the culture dish 2 flows directly into the other culture dish 2; when the tissue fluid flows to the position of the observation plate 4, the status of the mesenchymal stem cells in the tissue fluid can be observed in real time by the fill light 22 and the observation camera 21 and displayed on the display screen 23.

Claims

1. A mesenchymal stem cell quality detection device, comprising a scaffold (1) and two culture dishes (2), characterized in that: The two culture dishes (2) are symmetrically distributed on the left and right sides of the support (1); The support (1) is provided with a circulation mechanism, and the circulation mechanism is used to connect the two culture dishes (2) so that the tissue fluids in the two culture dishes (2) can circulate with each other; The bracket (1) is symmetrically provided with docking mechanisms on the left and right sides, and the docking mechanisms are used to seal and dock the culture dish (2) with the circulation mechanism; The support (1) is provided with a detection mechanism, and the detection mechanism is used to detect the quality of the mesenchymal stem cells in the tissue fluid at the position inside the support (1) by observing the tissue fluid flowing to the position inside the support (1) when the tissue fluid in the culture dish (2) circulates in the circulation mechanism; The circulation mechanism comprises a support plate (3), the support plate (3) is fixedly connected to the bracket (1), and the support plate (3) is located in the middle of the bracket (1); An observation plate (4) is fixedly connected to the upper position of the support plate (3), and the front and rear positions of the observation plate (4) are both rotatably connected to flow tubes (5), and the flow tubes (5) are rotatably connected to the bracket (1); the right end of the front flow tube (5) and the left end of the rear flow tube (5) are both rotatably connected to connecting tubes (6), and the connecting tubes (6) on the left and right sides are respectively located above the culture dishes (2) on the left and right sides; The detection mechanism comprises an observation camera (21), the observation camera (21) is located above the observation plate (4) and is fixedly mounted on the bracket (1); the observation plate (4) is made of transparent glass and a fill light (22) is provided below the observation plate (4), the fill light (22) is fixedly connected to the support plate (3); a display screen (23) is fixedly connected above the bracket (1), the display screen (23) is used to display an image observed by the observation camera (21) in real time; a diversion mechanism is provided on the bracket (1), the diversion mechanism is used to flow tissue fluid in one culture dish (2) into another culture dish (2); The flow guide mechanism comprises two cylinders (24), the two cylinders (24) are respectively located at the front and rear sides of the bracket (1) and the two cylinders (24) are rotatably connected to the bracket (1); the bottom ends of the front cylinder (24) and the bottom ends of the rear cylinder (24) are respectively rotatably connected to the flow pipes (5) at the front and rear sides; bevel gears (25) are symmetrically rotatably connected to the left and right sides of the flow pipe (5), the two bevel gears (25) are fixedly connected to each other and the surfaces of the two bevel gears (25) are meshed with bevel gear rings (26), and the two bevel gear rings (26) are respectively fixedly connected to the connecting pipe (6) and the bracket (1).

2. The mesenchymal stem cell quality detection device according to claim 1, characterized in that: The observation plate (4) has a flat inner cavity, and the front and rear flow tubes (5) are both connected to the inner cavity of the observation plate (4).

3. The mesenchymal stem cell quality detection device according to claim 1, characterized in that: The docking mechanism comprises a docking tube (7), the docking tube (7) being located directly above the culture dish (2) and fixedly connected to the connecting tube (6); a sealing ring (8) being fixedly connected to the bottom of the docking tube (7), and the upper end of the culture dish (2) being in contact with the sealing ring (8); a fastening mechanism being provided on the docking tube (7), and the fastening mechanism being used to fix the culture dish (2) and exert an upward pressing force thereon.

4. The mesenchymal stem cell quality detection device according to claim 3, characterized in that: The fastening mechanism comprises a sliding ring (9), which is sleeved on the docking tube (7); the surface of the docking tube (7) is provided with a thread and a plurality of vertical sliding grooves are opened on the surface of the docking tube (7); the sliding ring (9) is clearance-matched with the docking tube (7) and is slidingly connected to the vertical sliding grooves on the surface of the docking tube (7); four connecting blocks (10) are fixedly connected to the sliding ring (9), a friction block (11) is provided at the inner position of the connecting block (10), two connecting rods (12) are provided between the friction block (11) and the connecting block (10), and the two ends of the two connecting rods (12) are rotatably connected to the friction block (11) and the connecting block (10) respectively; a driving mechanism is provided on the sliding ring (9), and the driving mechanism is used to drive the friction block (11) to fit with the side wall of the culture dish (2) and drive the sliding ring (9) to move upward after the friction block (11) fits with the side wall of the culture dish (2).

5. The mesenchymal stem cell quality detection device according to claim 4, characterized in that: The thread on the docking sleeve (7) is self-locking; the inner portion of the friction block (11) is made of rubber; and the two connecting rods (12) are of equal length and parallel to each other.

6. The mesenchymal stem cell quality detection device according to claim 4, characterized in that: The driving mechanism includes a first rotating ring (13), the first rotating ring (13) is sleeved on the docking sleeve (7) and the first rotating ring (13) is threadedly connected to the docking sleeve (7); the first rotating ring (13) is located above the sliding ring (9) and the first rotating ring (13) is rotatably connected to the sliding ring (9); a sliding block (14) is slidably connected to the connecting block (10), a sliding rod (15) is provided on the sliding block (14), the sliding rod (15) is slidably connected to the sliding block (14), and an inner end of the sliding rod (15) is fixedly connected to the friction block (11); A spring (16) is sleeved on the outside of the slide rod (15), one end of the spring (16) is fixedly connected to the slide rod (15) and the other end is fixedly connected to the sliding block (14); an extrusion mechanism is provided on the first rotating ring (13), and the extrusion mechanism is used to squeeze the slide rod (15) toward the outside position when the first rotating ring (13) rotates, and cancel the squeezing of the slide rod (15) when the first rotating ring (13) rotates in the other direction.

7. The mesenchymal stem cell quality detection device according to claim 6, characterized in that: The extrusion mechanism comprises four arc frames (17), and the four arc frames (17) are fixedly connected to the four connecting blocks (10) respectively; a second rotating ring (18) is provided at the bottom position of the first rotating ring (13), the second rotating ring (18) is slidably connected to the four arc frames (17), and the second rotating ring (18) is fixedly connected to the first rotating ring (13); four first extrusion blocks (19) are fixedly connected to the second rotating ring (18) at positions close to the four sliding rods (15); a second extrusion block (20) is fixedly connected to one end of the outer side of the sliding rod (15), and the second extrusion block (20) is located on the moving track of the first extrusion block (19).

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