Bacteria detection module

Abstract

The invention relates to the technical field of bacterium detection, particularly provides a bacterium detection module, and aims to solve the problems that a bacterium quantity detection method in the prior art is long in period and cannot be used for civil use. In order to achieve the purpose, the bacterium detection module comprises an excitation light source and a fluorescence detection device, the excitation light source is configured to be capable of emitting excitation light after being electrified, and the excitation light can excite microorganisms carried by a to-be-detected target to emit fluorescence; the fluorescence detection device is configured to be capable of detecting fluorescence emitted by microorganisms carried by the to-be-detected target after the to-be-detected target is irradiated by the excitation light after being electrified, and outputting a detection result. Through the arrangement of the excitation light source and the fluorescence detection device, the excitation light source emits the excitation light to irradiate the to-be-detected target, and the fluorescence detection device detects the fluorescence emitted after the microorganisms in the to-be-detected target are irradiated by the excitation light, so that the quantity of the microorganisms carried by the to-be-detected target can be determined without sampling and culturing; the method can be widely applied to various scenes in daily life.

Description

Technical Field

[0001] The present invention relates to the technical field of bacteria detection, and specifically provides a bacteria detection module. Background Art

[0002] There are more or less microorganisms in the air. These microorganisms usually attach to dust or droplets and are suspended in the air with dust and droplets. Especially in environments with high humidity, high dust, poor ventilation, and insufficient sunlight, the content of microorganisms in the air is particularly high and their survival time is relatively long. The presence of microorganisms can make the air a medium for spreading respiratory diseases, which seriously endangers people's health.

[0003] For the sake of people's health, it is necessary to detect the number of microorganisms in the air, and take corresponding measures to reduce the number of microorganisms in time when the number of microorganisms exceeds the standard. At present, when detecting the number of microorganisms in the air, it is usually necessary to first use a specific sampler to sample, and then culture the sampled air sample through a culture medium, and then count it. Since a culture medium that can culture all microorganisms has not yet been found, the number of bacteria and fungi is currently used as the number of microorganisms in the air. Commonly used detection methods for detecting bacteria mainly include sedimentation plate method, liquid impact method, impact plate method and filter membrane method. Among them, the sedimentation plate method is to place a plate containing agar culture medium at the location to be tested, open the plate cover and expose it for a certain period of time, and then culture it and count the number of colonies. The liquid impact method is also called the absorption tube method, which quickly absorbs a certain amount of air into the absorption liquid in the tube, and then takes a certain amount of this liquid, dilutes it and culture it on the plate, counts the number of colonies or separates pathogenic microorganisms. The impact plate method is to suck a certain amount of air and quickly impact one or several, rotating or non-rotating plate culture medium surfaces, and then culture the plate and count the number of growing colonies. The membrane filter method is to pass a certain amount of air through a special filter membrane supported on the filter, such as a nitrocellulose filter membrane, so that the dust particles carrying microorganisms adhere to the surface of the filter membrane, and then wash the dust particles trapped on the filter membrane into a suitable solution, and then absorb a certain amount of this solution to determine the number of bacteria.

[0004] However, the above detection methods usually require the use of a specific sampler to collect samples and then culture them in a culture medium, which means that the detection of bacterial counts can only be carried out in laboratories with testing qualifications. During the test, professionals are required to collect samples on site and then bring the samples back to the laboratory for culture and testing, which has the disadvantages of long detection cycles and cannot be used for civilian purposes.

[0005] Accordingly, the art needs a new technical solution to solve the above problems. Summary of the invention

[0006] The present invention aims to solve the above technical problems, that is, to solve the problems that the detection method of the number of bacteria in the prior art has a long cycle and is not applicable to civilian use.

[0007] The present invention provides a bacteria detection module, which includes an excitation light source and a fluorescence detection device. The excitation light source is configured to emit excitation light after being powered on, and the excitation light can excite microorganisms carried in a target to be detected to emit fluorescence. The fluorescence detection device is configured to detect the fluorescence emitted by the microorganisms carried by the target to be detected after the target is irradiated with the excitation light after being powered on, and output the detection result.

[0008] In the preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module also includes a reflecting unit, the excitation light source is located on a first side of the reflecting unit, the fluorescence detection device and the target to be detected are located on opposite sides of the first side, and the reflecting unit is configured to at least allow the light in the excitation light that can excite the microorganism to emit fluorescence to pass through, and to reflect the fluorescence emitted by the microorganism to the fluorescence detection device.

[0009] In the preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module also includes a first light processing unit, which is arranged between the excitation light source and the reflection unit, and the first light processing unit is configured to be able to adjust the irradiation direction and / or energy distribution of the excitation light so that the light in the excitation light that can excite the microorganism to emit fluorescence can better illuminate the target to be detected.

[0010] In the preferred technical solution of the above-mentioned bacteria detection module, the first light processing unit includes a first lens, the first lens is arranged between the excitation light source and the reflection unit, and the first lens is configured to adjust the excitation light emitted by the excitation light source into light irradiated in a horizontal direction; and / or

[0011] The first light processing unit further includes a light homogenizer, which is disposed between the excitation light source and the reflection unit, and is configured to evenly distribute energy of each wavelength in the light passing through the light homogenizer; and / or

[0012] The first light processing unit further includes a first filter, which is disposed between the excitation light source and the reflection unit, and is configured to at least allow light that can excite the microorganism to emit fluorescence to pass through.

[0013] In the preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module also includes a second light processing unit, which is arranged between the reflection unit and the fluorescence detection device, and the second light processing unit is configured to filter and / or focus the fluorescence reflected from the reflection unit and transmit it to the fluorescence detection device.

[0014] In the preferred technical solution of the above-mentioned bacteria detection module, the second light processing unit includes a second filter, the second filter is arranged between the reflection unit and the fluorescence detection device, and the second filter is configured to at least allow the fluorescence emitted by the microorganism to pass through; and / or

[0015] The second light processing unit includes a second lens, which is disposed between the second filter of the reflection unit and the fluorescence detection device, and the second lens is configured to at least focus the fluorescence reflected from the reflection unit onto the fluorescence detection device.

[0016] In the preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module has a first chamber, the excitation light source, the fluorescence detection device and the reflection unit are arranged in the first chamber, the excitation light source and the fluorescence detection device are respectively arranged on both sides of the reflection unit, and a first connecting structure is provided on the first chamber, and at least a part of the reflection unit is aligned with the first connecting structure, so that at least the light in the excitation light that can excite the microorganism to emit fluorescence can be irradiated to the target to be detected through the first connecting structure after passing through the reflection unit, and the fluorescence emitted by the microorganism can be reflected by the reflection unit to the fluorescence detection device after passing through the first connecting structure.

[0017] In a preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module further has a second chamber, the target to be detected is arranged in the second chamber, and the first chamber and the second chamber are connected to each other through the first connecting structure.

[0018] In the preferred technical solution of the above-mentioned bacteria detection module, the target to be detected is the air in the object to be detected, and a second connecting structure and a third connecting structure are arranged on the second chamber. The air in the object to be detected enters the second chamber through the second connecting structure and then is discharged from the second chamber through the third connecting structure.

[0019] In a preferred technical solution of the above-mentioned bacteria detection module, the bacteria detection module further includes a fan, and the fan is configured to allow air in the object to be detected to enter the second chamber via the second connecting structure.

[0020] In the technical solution of the present invention, the bacteria detection module includes an excitation light source and a fluorescence detection device, the excitation light source is configured to emit excitation light after being powered on, the excitation light can excite the microorganisms carried by the target to be tested to emit fluorescence, and the fluorescence detection device is configured to detect the fluorescence emitted by the microorganisms carried by the target to be tested after being irradiated with the excitation light after being powered on, and output the detection result. Since microorganisms, especially bacteria, usually have various fluorescent groups, fluorescence will be generated under the induction of the excitation light. That is to say, when it is necessary to detect the number of microorganisms in the target to be tested, for example, when detecting the microbial content in the air, the target to be tested is irradiated with the excitation light emitted by the excitation light source, and then the fluorescence emitted by the microorganisms carried by the target to be tested is detected by the fluorescence detection device, and the number of microorganisms in the target to be tested can be obtained. Through such a setting, the excitation light source and the fluorescence detection device are powered on, and the excitation light emitted by the excitation light source is irradiated on the target to be tested, and then the number of microorganisms carried by the target to be tested can be determined by the fluorescence detection device, without sampling and culturing, and the result can be obtained by directly detecting, and the detection process is simple and fast, and can be widely used in various scenes in daily life.

[0021] Furthermore, the bacteria detection module also includes a reflection unit, which is arranged between the excitation light source and the target to be detected. The reflection unit is arranged to at least allow the light in the excitation light that can excite the microorganism to emit fluorescence to pass through, and can reflect the fluorescence emitted by the microorganism to the fluorescence detection device. In this way, it will not affect the light in the excitation light that can excite the microorganism to emit fluorescence to pass through the emission unit to illuminate the target to be detected, ensuring that the microorganisms in the target to be detected can emit fluorescence, and can also reflect the fluorescence emitted by the microorganism to the fluorescence detection device, ensuring that the fluorescence emitted by the microorganism can smoothly and fully reach the fluorescence detection device, and then the number of microorganisms in the target to be detected is detected by the fluorescence detection device. In addition, the reflection unit can also reflect the light in the excitation light that will affect the fluorescence detection, so as to avoid the light in other bands in the excitation light from affecting the detection of fluorescence. It can be seen that through the setting of the emission unit, the accuracy of the bacteria detection module can be effectively improved and the user experience can be improved.

[0022] Furthermore, the bacteria detection module also includes a first light processing unit, which is disposed between the excitation light source and the reflection unit, and is configured to adjust the excitation light so that the light in the excitation light that can excite the microorganism to emit fluorescence can be better irradiated to the target to be detected. In other words, the first light processing unit adjusts the direction of the excitation light, the energy of each wavelength in the light, filtering, etc., and adjusts the excitation light to irradiate in a parallel direction, with balanced energy distribution, and mainly including (or only including) light that can excite the microorganism to emit fluorescence to irradiate the target to be detected. In this way, the excitation light emitted by the excitation light source can be fully utilized, the stray light in the fluorescence emitted by the microorganism after being excited can be reduced, and the accuracy of the bacteria detection module can be improved.

[0023] Furthermore, the bacteria detection module also includes a second light processing unit, which is disposed between the reflection unit and the fluorescence detection device, and is configured to adjust the fluorescence reflected from the reflection unit and transmit it to the fluorescence detection device. In other words, the second light processing unit filters and adjusts the direction of the fluorescence reflected from the reflection unit, filters out stray light in the fluorescence, and collects the fluorescence at the fluorescence detection device, so that the fluorescence can be detected more accurately, thereby improving the accuracy of the bacteria detection module.

[0024] Furthermore, the bacteria detection module also includes a first chamber and a second chamber, an excitation light source, a fluorescence detection device and a reflection unit are arranged in the first chamber, the target to be detected is arranged in the second chamber, and the first chamber and the second chamber are connected to each other through a first connecting structure. During detection, the excitation light reflected by the excitation light source is irradiated from the first connecting structure into the second chamber and onto the target to be detected located in the second chamber, and the microorganisms carried in the target to be detected emit fluorescence after being irradiated, and the fluorescence passes through the first connecting structure into the first chamber, and is then reflected by the reflection unit to the fluorescence detection device. In this way, the number of microorganisms carried by the target to be detected can be detected without sampling and culturing, and the target to be detected can be placed in the second chamber, while at the same time, the target to be detected can be prevented from contaminating the excitation light source, the fluorescence detection device and the reflection unit, thereby improving the accuracy of the bacteria detection module.

[0025] Furthermore, the target to be tested is the air in the object to be tested, and the bacteria detection module also includes a third chamber and a fan. The fan is arranged in the third chamber, and the third chamber is connected to the second chamber through a third connecting structure. Under the action of the fan, the air in the object to be tested enters the second chamber via the second connecting structure on the second chamber, then enters the third chamber via the third connecting structure, and then is discharged. When the air enters the second chamber, it is detected as the target to be tested, and the number of microorganisms carried in the air is obtained, and then the content of microorganisms in the object to be tested can be detected. In other words, there is no need for sampling and culturing. The air in the object to be tested is introduced into the second chamber by a fan, and the number of microorganisms in the object to be tested can be detected. It is simple, fast and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings, in which:

[0027] Figure 1 is a structural diagram of a bacteria detection module according to an embodiment of the present invention (I);

[0028] Figure 2 is a structural diagram of a bacteria detection module according to an embodiment of the present invention (II);

[0029] Figure 3 It is a cross-sectional view of a bacteria detection module according to an embodiment of the present invention.

[0030] List of reference numerals:

[0031] 1. Excitation light source; 2. Fluorescence detection device; 3. Dichroic mirror; 4. First chamber; 41. Horizontal part; 42. Vertical part; 5. Second chamber; 6. Third chamber; 7. Fan; 8. First light processing unit; 81. First lens; 82. Light homogenizer; 83. First filter; 9. Second light processing unit; 91. Second filter; 92. Second lens; 10. Air supply duct; 11. Ventilation duct. DETAILED DESCRIPTION

[0032] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0033] It should be noted that in the description of the present invention, the terms "upper", "lower", "left", "right", "inner", "outer" and the like indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for the convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0034] In addition, it should be noted that in the description of this application, unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0035] At present, to detect the number of bacteria in the air, it is usually necessary to first sample with a specific sampler, and then culture the sample through a culture medium, and the culture usually takes several hours or days. After the culture is completed, the culture fluid is tested again, so that the number of bacterial colonies in the air can be detected. The entire detection process is time-consuming and usually needs to be carried out in a laboratory with detection qualifications, and cannot be widely used in the civilian market. To this end, the bacteria detection module of the present invention emits excitation light through an excitation light source to illuminate the target to be tested, and then detects the fluorescence emitted by the target to be tested after being irradiated with the excitation light through a fluorescence detection device, so that the number of microorganisms carried by the target to be tested can be determined, without sampling and culturing, the detection process is simple and fast, and can be widely used in various scenes in daily life.

[0036] Refer to the following Figures 1 to 3 To illustrate possible implementations of the bacteria detection module of the present invention.

[0037] like Figures 1 to 3As shown, the bacteria detection module includes an excitation light source 1 and a fluorescence detection device 2. The excitation light source 1 is configured to emit excitation light after being powered on, and the excitation light can excite the microorganisms carried by the target to be tested to emit fluorescence. The fluorescence detection device 2 is configured to detect the fluorescence emitted by the microorganisms carried by the target to be tested after irradiating the target to be tested with the excitation light, and output the detection result. This is because there are many types of microorganisms carried in the target to be tested, and various microorganisms usually have various fluorescent groups. Most microorganisms (especially bacteria) have roughly the same fluorescent groups, mainly composed of porphyrin, coenzyme, NADH and flavin. These fluorescent groups will produce fluorescence under the induction of excitation light. However, due to the differences in the content of each fluorescent group, the structure of the microbial organism, and the degree to which different microorganisms absorb laser energy, these differences will produce different fluorescence signals under the irradiation of the excitation light. The fluorescence detection device 2 finally determines the content of the microorganisms carried in the target to be tested by analyzing the fluorescence characteristics and the fluorescence signals. That is to say, when it is necessary to detect the number of microorganisms in the target to be detected, the target to be detected is irradiated with the excitation light emitted by the excitation light source 1, and then the fluorescence emitted by the microorganisms is detected by the fluorescence detection device 2, so that the number of microorganisms in the target to be detected can be obtained.

[0038] Based on the bacteria detection module of the present invention, when it is necessary to detect the number of microorganisms carried in the target to be detected, the excitation light source 1 and the fluorescence detection device 2 are powered on, the excitation light source 1 emits excitation light, the target to be detected is irradiated with the excitation light, the microorganisms carried in the target to be detected will emit fluorescence, and the fluorescence is detected by the fluorescence detection device 2, so that the number of microorganisms carried in the target to be detected is determined, and the result can be obtained by directly performing the detection without sampling and culturing. The detection process is simple and fast, and can be widely used in various scenarios in daily life.

[0039] It should be noted that the fluorescence detection device 2 may include a photocell, a photoresistor, a photomultiplier tube, a diode array detector, a charge coupled device or a photon counter, etc. Without departing from the basic principles of the present application, those skilled in the art may flexibly select the specific type of the fluorescence detection device 2 according to the specific application scenario, as long as it can accurately detect the received fluorescence.

[0040] In a possible embodiment, the excitation light source 1 is a laser emitter or a laser, that is, a device capable of emitting laser light. In other words, the excitation light emitted by the excitation light source 1 is a laser, which has the characteristics of directional emission, extremely high brightness, and monochromaticity. The laser can excite fluorescent groups in microorganisms and generate fluorescence. It should be noted that the laser can be a helium-neon laser, a laser diode, etc. The present invention does not limit the specific type of the laser, as long as the laser can emit a laser capable of emitting fluorescence.

[0041] It should be noted that the excitation light source 1 can also be an ultraviolet lamp or LED lamp that can emit UVA ultraviolet rays. Obviously, the excitation light source 1 can also be a device that can emit excitation light containing a wavelength of 340nm to 405nm, that is, an excitation light containing a wavelength between 340nm and 405nm. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific type of excitation light source 1 according to the specific application scenario, as long as it can emit light that can excite microorganisms to emit fluorescence.

[0042] like Figures 1 to 3 As shown, the bacteria detection module also includes a reflection unit, the excitation light source 1 is located on a first side of the reflection unit, and the fluorescence detection device 2 and the target to be detected are located on the opposite side of the first side, that is, the excitation light source 1, the fluorescence detection device 2 and the target to be detected are respectively located on both sides of the reflection unit. The reflection unit is configured to at least allow the light in the excitation light that can excite the microorganism to emit fluorescence to pass through, and can reflect the fluorescence emitted by the microorganism to the fluorescence detection device 2, so that it will not affect the light in the excitation light that can excite the microorganism to emit fluorescence to pass through the emission unit to the target to be detected, and can reflect the fluorescence emitted by the microorganism to the fluorescence detection device 2, and at the same time, it can also reflect the light in the excitation light that will affect the fluorescence detection, effectively improving the accuracy of the bacteria detection module.

[0043] like Figures 1 to 3 As shown, the bacteria detection module has a first chamber 4 and a second chamber 5, the excitation light source 1, the fluorescence detection device 2 and the reflection unit are arranged in the first chamber 4, and the target to be detected is arranged in the second chamber 5. The first chamber 4 includes a horizontal part 41 and a vertical part 42, and is roughly an L-shaped structure. The excitation light source 1 is arranged at one end of the horizontal part 41 away from the vertical part 42 (roughly Figure 3 The fluorescence detection device 2 is arranged at the end of the vertical portion 42 away from the horizontal portion 41 (approximately Figure 3The upper end of the first chamber 4 in the middle). That is, the excitation light source 1 is arranged at a position close to the right end of the horizontal part 41, and the fluorescence detection device 2 is arranged at a position close to the upper end of the vertical part 42. The reflection unit is a dichroic mirror 3, which is also called a dual-band mirror, a dual-wavelength mirror, or a dichroic reflector. It refers to a mirror with significantly different reflection or transmission characteristics at two different wavelengths, that is, it can allow a part of the wavelengths of light to pass through and reflect a part of the wavelengths of light, for example, allowing light with a wavelength between 340nm and 405nm to pass through and reflecting light with a wavelength between 405nm and 600nm, etc. The dichroic mirror 3 is arranged at the intersection of the horizontal part 41 and the vertical part 42, and the angle between the dichroic mirror 3 and the horizontal direction is 45°. The second chamber 5 is roughly rectangular, and its size in the vertical direction is roughly consistent with the size of the horizontal part 41 of the first chamber 4 in the vertical direction. The second chamber 5 is arranged on the left side of the first chamber 4, and its right side wall shares a part of the side wall with the right side wall of the first chamber 4. A first connecting structure (not shown) is provided on the left side wall of the first chamber 4, through which the first chamber 4 and the second chamber 5 can be connected. At least a portion of the dichroic mirror 3 is aligned with the first connecting structure. In this way, when the bacteria detection module is used for detection, the first side (roughly Figure 3 After the excitation light source 1 is powered on, it emits excitation light, which reaches the dichroic mirror 3. The light in the excitation light that can excite the microorganism to emit fluorescence passes through the dichroic mirror 3. For example, the light with a wavelength between 340nm and 405nm passes through the dichroic mirror 3 and reaches the opposite side of the first side of the dichroic mirror 3 (approximately Figure 3 The first connecting structure is used to illuminate the target to be detected, and then the first connecting structure is used to illuminate the target to be detected, so that the microorganisms carried in the target to be detected emit fluorescence. Another part of the excitation light is reflected by the dichroic mirror 3, for example, the light with a wavelength of 405nm-600nm is reflected and will not enter the left side of the dichroic mirror 3, so as to prevent this part of the light from entering the position where the fluorescence detection device 2 is located and affecting the accuracy of its detection results. The microorganisms carried in the target to be detected emit fluorescence after being excited by the excitation light. The fluorescence passes through the first connecting structure and enters the first chamber 4. It is reflected by the dichroic mirror 3 to the fluorescence detection device 2 located on the upper left side of the dichroic mirror 3 and arranged near the upper end of the first chamber 4. The fluorescence characteristics are detected and analyzed by the fluorescence detection device 2, and then the number of microorganisms in the target to be detected is determined. Through the reflection of the dichroic mirror 3, the fluorescence generated by the microorganisms after being excited can be reflected to the fluorescence detection device 2, ensuring that the fluorescence detection device 2 can receive and detect all the fluorescence emitted by the microorganisms. It can be seen that the setting of the dichroic mirror 3 can effectively improve the accuracy of the bacteria detection module and enhance the user experience.

[0044] It should be noted that the reflection unit may also be a trichromatic mirror, a prism or other lenses that can allow part of the light to pass through or reflect part of the light.

[0045] like Figures 1 to 3 As shown and in accordance with Figure 3 In the orientation shown, the bacteria detection module also includes a first light processing unit 8, which includes a first lens 81, a light homogenizer 82 and a first filter 83 arranged in sequence from right to left in a direction away from the excitation light source 1, and the first lens 81, the light homogenizer 82 and the first filter 83 are arranged on the same optical axis. Among them, the first lens 81 includes two collimating lenses, which can be a plano-convex lens or a biconvex lens. The two collimating lenses are arranged in sequence along the irradiation of the excitation light, and their upper and lower ends are respectively abutted against the inner wall of the first chamber 4. Through these two collimating lenses, the direction of all excitation light emitted by the excitation light source 1 can be adjusted to be adjusted to light irradiated in the horizontal direction. The upper and lower ends of the light homogenizer 82 are respectively abutted against the inner wall of the first chamber 4. Through the light homogenizer 82, the energy of each wavelength in the excitation light irradiated in the horizontal direction after adjustment by the collimating lens can be evenly distributed, and converted into a light spot with uniform energy distribution. The first filter 83 is an excitation filter, which is usually made by adding special dyes to plastic or glass. The filter can absorb part of the light except the wavelength band that can excite microorganisms to emit fluorescence. In this way, under the action of the first lens 81, the light homogenizer 82 and the first filter 83, the excitation light emitted by the excitation light source 1 is adjusted to mainly include light that can excite microorganisms to emit fluorescence and irradiates in the horizontal direction, reducing the interference of other stray lights, and more purely and fully irradiating the target to be measured in the second chamber 5, so that the microorganisms carried in the target to be measured can be better stimulated to emit fluorescence.

[0046] It should be noted that the first light processing unit 8 may also include only one or two of the first lens 81, the light homogenizer 82 and the first filter 83. Of course, the bacteria detection module may not include the first light processing unit 8, and the excitation light emitted by the excitation light source 1 directly illuminates the target to be measured and can also excite the microorganisms carried in the target to be measured to emit fluorescence. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific setting form of the bacteria detection module according to the specific application scenario, as long as the bacteria detection module can accurately detect the number of microorganisms carried in the target to be measured.

[0047] It should be noted that in this application, the horizontal direction is approximately Figure 3 In the direction extending left and right.

[0048] like Figures 1 to 3 As shown and in accordance with Figure 3In the orientation shown, the bacteria detection module also includes a second light processing unit 9, which includes a second filter 91 and a second lens 92 arranged in sequence from bottom to top in a direction away from the dichroic mirror 3, and the second filter 91 and the second lens 92 are arranged on the same optical axis. Since the light passing through the dichroic mirror 3 from right to left includes other stray light in addition to the light that can excite the microorganism to emit fluorescence, after this part of the light irradiates the target to be measured, the light reaching the dichroic mirror 3 naturally includes other stray light and part of the light reflected back by the target to be measured that can excite the microorganism to emit fluorescence in addition to fluorescence. After this part of the light reaches the dichroic mirror 3, it is reflected to the second filter 91, and the stray light except for fluorescence can be filtered out as much as possible through the second filter 91. The second lens 92 is a fluorescence collection lens, which is similar to a collimating lens, and parallel light or scattered light can be converged to its focus after passing through the second lens 92. In this way, under the action of the second filter 91 and the second lens 92, the scattered fluorescence containing stray light reflected by the dichroic mirror 3 is adjusted to light that only includes fluorescence and converges to the focal point, thereby avoiding the interference of stray light. The fluorescence is detected by the fluorescence detection device 2 set at the focal point, and the content of microorganisms in the target to be tested can be accurately determined.

[0049] It should be noted that the second light processing unit 9 may also include only one of the second lens 92 box and the second filter 91. Of course, the bacteria detection module may not include the second light processing unit 9. When the fluorescence generated by the microorganisms after being excited is reflected by the dichroic mirror 3 and reaches the fluorescence detection device 2, the fluorescence detection device 2 can also detect and analyze the fluorescence. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific setting form of the bacteria detection module according to the specific application scenario, as long as the bacteria detection module can accurately detect the number of microorganisms carried in the target to be detected.

[0050] In a possible implementation, the target to be measured is the air in the target to be measured, for example, the air in the indoor space, in the car, in the train. Of course, the target to be measured can also be the surface of the target to be measured on the table, the surface of the appliance, the surface of the equipment, the dirty solution, the liquid to be measured in the water flow.

[0051] The following takes the air in an indoor space as an example to further illustrate possible implementations of the bacteria detection module of the present invention.

[0052] like Figures 1 to 3 As shown and in accordance with Figure 3In the orientation shown, the bacteria detection module also includes a third chamber 6 and a fan 7 disposed in the third chamber 6. The third chamber 6 is roughly rectangular, and its lower side wall is shared with the upper side wall of the second chamber 5, and the right side wall is shared with a part of the left side wall of the first chamber 4. The size of the third chamber 6 in the horizontal direction is roughly the same as the size of the second chamber 5 in the horizontal direction, and the sum of the sizes of the second chamber 5 and the third chamber 6 in the vertical direction is roughly the same as the size of the vertical part 42 of the first chamber 4 in the vertical direction. A second connecting structure (not shown) is provided on the lower side wall of the second chamber 5, and a third connecting structure (not shown) is provided on the upper side wall. The second chamber 5 is also provided with an air supply pipe 10, one end of which is connected to the external environment, and the other end is connected to the second chamber 5 through the second connecting structure, and the second chamber 5 is connected to the third chamber 6 through the third connecting structure. The third chamber 6 is also provided with a ventilation structure (not shown) and a ventilation pipe 11, one end of the ventilation pipe 11 is connected to the external environment, and the other end is connected to the third chamber 6 through the ventilation structure. When it is necessary to detect the number of microorganisms in the air of the indoor space, under the action of the fan 7, the air in the indoor space is introduced into the second chamber 5 through the second connecting structure, then enters the third chamber 6 through the third connecting structure, and is discharged from the bacteria detection module through the ventilation structure. At the same time, the excitation light emitted by the excitation light passes through the first light processing unit 8 and the dichroic mirror 3, passes through the first connecting structure and irradiates the second chamber 5, irradiating the air introduced into the second chamber 5, and the microorganisms carried in the air are excited to emit fluorescence, which passes through the first connecting structure and is reflected by the dichroic mirror 3, and then is transmitted by the second light processing unit 9 to the fluorescence detection device 2. The fluorescence detection device 2 detects and analyzes the characteristics of the fluorescence, and then the number of microorganisms in the air of the indoor space can be determined. Obviously, the air supply pipe 10 may not be provided on the second chamber 5, and the ventilation pipe 11 may not be provided on the third chamber 6.

[0053] It should be noted that the bacteria detection module may not include the third chamber 6. In this case, the fan 7 may be arranged in the second chamber 5. Of course, the bacteria detection module may not include the fan 7, but the bacteria detection module may be arranged in the air duct, for example, the air duct of the air conditioner, the circulating air duct of the washer-dryer or the dryer, etc. Taking the bacteria detection module arranged in the air duct of the air conditioner as an example, the air in the indoor space enters the air duct under the action of the fan of the air conditioner, enters the second chamber 5 through the second ventilation structure, and then is discharged into the air duct through the third ventilation structure. The excitation light source 1 emits excitation light to illuminate the air entering the second chamber 5, that is, the air in the indoor space can be detected to obtain the number of microorganisms in the air. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific setting method of the fan 7 according to the specific application scenario, as long as the number of microorganisms carried in the air of the object to be tested can be detected.

[0054] It should be noted that the bacteria detection module may not include the second chamber 5, and the excitation light emitted by the excitation light can be irradiated from the first connecting structure to the target to be detected outside the bacteria detection module, and the fluorescence generated by the microorganisms carried by the target to be detected after being excited can pass through the first connecting structure and be reflected by the dichroic mirror 3 to the fluorescence detection device 2. Of course, the bacteria detection module may not include the first chamber 4. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific setting mode of the bacteria detection module according to the specific application scenario, as long as the number of microorganisms carried in the target to be detected can be detected by the bacteria detection module.

[0055] It should be noted that the first connection structure can be a hole in the shape of a circle, rectangle, square, polygon, etc. The second connection structure, the third connection structure and the ventilation structure can be holes in the shape of a circle, rectangle, square, polygon, etc., or can be arranged by a plurality of small holes in the shape of a circle, rectangle, square, polygon, etc., or can be hollowed out in various possible shapes. Without departing from the basic principles of the present application, those skilled in the art can flexibly select the specific setting form of the first connection structure, the second connection structure, the third connection structure and the ventilation structure according to the specific application scenario, as long as the connection between the first chamber 4 and the second chamber 5, the connection between the second chamber 5 and the third chamber 6, the connection between the second chamber 5 and the outside, and the connection between the third chamber 6 and the outside can be achieved.

[0056] In summary, in the preferred technical solution of the present invention, the bacteria detection module includes an excitation light source 1 and a fluorescence detection device 2. The excitation light source 1 emits light that can excite the microorganisms carried by the target to be detected to emit fluorescence, and the fluorescence detection device 2 detects the fluorescence and outputs the result. In this way, when it is necessary to detect the number of microorganisms carried by the target to be detected, the excitation light emitted by the excitation light source 1 irradiates the target to be detected, and then the number of microorganisms carried by the target to be detected can be determined by the fluorescence detection device 2. No sampling or cultivation is required, and the result can be obtained by directly performing the detection. The detection process is simple and fast, and can be widely used in various scenes in daily life. The bacteria detection module includes a reflection unit arranged between the excitation light source 1 and the target to be detected. The reflection unit can allow the light in the excitation light that can excite the microorganisms to emit fluorescence to pass through, and can reflect the fluorescence emitted by the microorganisms to the fluorescence detection device 2, so that the microorganisms in the target to be detected can be fully excited, and the fluorescence emitted by the microorganisms can be fully detected, thereby effectively improving the accuracy of the bacteria detection module. The bacteria detection module comprises a first chamber 4, a second chamber 5 and a third chamber 6. The first chamber 4 is connected to the second chamber 5 via a first connecting structure, the second chamber 5 is connected to the third chamber 6 via a third connecting structure, a fan 7 is arranged in the third chamber 6, and the target to be detected is the air in the object to be detected. Under the action of the fan 7, the air in the object to be detected enters the second chamber 5 via the second connecting structure and is then discharged through the third chamber 6. When the air flows through the second chamber 5, it is irradiated with the excitation light emitted by the excitation light source 1 arranged in the first chamber 4 to emit fluorescence. The number of microorganisms carried in the air can be determined by detecting the fluorescence characteristics using the fluorescence detection device 2.

[0057] Of course, the above-mentioned replaceable implementations, as well as the replaceable implementations and the preferred implementations, can be used in a cross-coordinated manner to combine new implementations to suit more specific application scenarios.

[0058] In addition, those skilled in the art will appreciate that, although some embodiments described herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present invention and form different embodiments. For example, in the claims of the present invention, any one of the claimed embodiments may be used in any combination.

[0059] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A bacteria detection module, characterized in that: The bacteria detection module includes an excitation light source and a fluorescence detection device. The excitation light source is configured to emit excitation light after being powered on, and the excitation light can excite the microorganisms carried in the target to be detected to emit fluorescence. The fluorescence detection device is configured to detect the fluorescence emitted by the microorganisms carried by the target after being irradiated with the excitation light after being powered on, and output the detection result.

2. The bacteria detection module according to claim 1, characterized in that: The bacteria detection module also includes a reflecting unit, the excitation light source is located on a first side of the reflecting unit, the fluorescence detection device and the target to be detected are located on the opposite side of the first side, and the reflecting unit is configured to at least allow the light in the excitation light that can excite the microorganism to emit fluorescence to pass through, and to reflect the fluorescence emitted by the microorganism to the fluorescence detection device.

3. The bacteria detection module according to claim 2, characterized in that: The bacteria detection module also includes a first light processing unit, which is arranged between the excitation light source and the reflection unit. The first light processing unit is configured to adjust the irradiation direction and / or energy distribution of the excitation light so that the light in the excitation light that can excite the microorganism to emit fluorescence can better illuminate the target to be detected.

4. The bacteria detection module according to claim 3, characterized in that: The first light processing unit includes a first lens, the first lens is arranged between the excitation light source and the reflection unit, and the first lens is configured to adjust the excitation light emitted by the excitation light source into light irradiated in a horizontal direction; and / or The first light processing unit further includes a light homogenizer, which is disposed between the excitation light source and the reflection unit, and is configured to evenly distribute energy of each wavelength in the light passing through the light homogenizer; and / or The first light processing unit further includes a first filter, which is disposed between the excitation light source and the reflection unit, and is configured to at least allow light that can excite the microorganism to emit fluorescence to pass through.

5. The bacteria detection module according to claim 2, characterized in that: The bacteria detection module further includes a second light processing unit, which is disposed between the reflection unit and the fluorescence detection device, and is configured to filter and / or focus the fluorescence reflected from the reflection unit and transmit it to the fluorescence detection device.

6. The bacteria detection module according to claim 5, characterized in that: The second light processing unit comprises a second filter, the second filter is arranged between the reflection unit and the fluorescence detection device, and the second filter is configured to at least allow the fluorescence emitted by the microorganism to pass through; and / or The second light processing unit includes a second lens, which is disposed between the second filter of the reflection unit and the fluorescence detection device, and the second lens is configured to at least focus the fluorescence reflected from the reflection unit onto the fluorescence detection device.

7. The bacteria detection module according to any one of claims 2 to 6, characterized in that: The bacteria detection module has a first chamber, the excitation light source, the fluorescence detection device and the reflection unit are arranged in the first chamber, the excitation light source and the fluorescence detection device are respectively arranged on both sides of the reflection unit, and a first connecting structure is provided on the first chamber, and at least a part of the reflection unit is aligned with the first connecting structure, so that at least the light in the excitation light that can excite the microorganism to emit fluorescence can be irradiated to the target to be detected through the first connecting structure after passing through the reflection unit, and the fluorescence emitted by the microorganism can be reflected by the reflection unit to the fluorescence detection device after passing through the first connecting structure.

8. The bacteria detection module according to claim 7, characterized in that: The bacteria detection module further has a second chamber, the target to be detected is arranged in the second chamber, and the first chamber and the second chamber are connected to each other through the first connecting structure.

9. The bacteria detection module according to claim 8, characterized in that: The target to be measured is the air in the object to be measured. The second chamber is provided with a second connecting structure and a third connecting structure. The air in the object to be measured enters the second chamber through the second connecting structure and then is discharged from the second chamber through the third connecting structure.

10. The bacteria detection module according to claim 9, characterized in that: The bacteria detection module further includes a fan, and the fan is configured to allow air in the object to be detected to enter the second chamber via the second connecting structure.

Images