Light-activated chemiluminescence analyzer and method

Abstract

The invention belongs to the technical field of biomedical treatment, and particularly relates to a light-activated chemiluminescence analyzer and a method. The light-activated chemiluminescence analyzer comprises an incubation reaction module, a storage module, a pipetting arm module and a detection module, the storage module comprises a reagent storage module and a sample storage module, a reagent inlet and a sample inlet are formed in a shell of the light-activated chemiluminescence analyzer, and the storage module can be accessed through the reagent inlet and the sample inlet; the pipetting arm module comprises a pipetting arm and a pipetting arm driving device and is configured to inject the reagent and the sample in the storage module into the incubation reaction module; the incubation reaction module comprises an incubation reaction disc, a placing position for storing a reaction cup is arranged on the incubation reaction disc, and a reagent and a sample are injected into the reaction cup by the pipetting arm; the detection module is arranged above the incubation reaction disc and is used for detecting a detection signal sent out from the reaction cup; the reagent storage module comprises a reagent disc, the sample storage module comprises a sample disc, and the reagent disc and the sample disc are coaxially sleeved.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical technology, and in particular, relates to a photochemiluminescence analyzer and a photochemiluminescence analysis method. Background Art

[0002] Sample analysis refers to the analysis of the concentration of the substance to be tested in the blood sample by physical or chemical methods. Among the many types of sample analysis, chemiluminescence is an immunoassay method that has attracted much attention in recent years. It uses the light signal emitted by the chemical reaction between the substance to be tested in the blood sample and the effective substance in the detection reagent to determine the concentration of the substance to be tested.

[0003] Conventional chemiluminescence analyzers are mainly large instruments with high detection throughput. The reason is that the chemiluminescence method used by conventional chemiluminescence analyzers is generally based on magnetic microparticles, such as enzymatic chemiluminescence or electrochemiluminescence. These heterogeneous chemiluminescence methods require continuous cleaning during the reaction between the blood sample and the detection reagent to remove impurity interference. Therefore, a large amount of workspace is required to place various cleaning solutions and arrange liquid pipelines. This makes conventional chemiluminescence analyzers generally have the characteristics of large footprint and inconvenient movement.

[0004] Some small chemiluminescence analyzers have now appeared. However, this type of chemiluminescence analyzer generally faces a game between cost and ease of use. For small chemiluminescence analyzers based on heterogeneous chemiluminescence, a large amount of consumables are required to clean the liquid circuit, which reduces ease of use. This instrument usually uses bottled detection reagents and therefore uses a pipette needle to dispense the reagents. The user must frequently replenish the cleaning solution for cleaning impurities, maintain the cleaning liquid circuit of the pipette needle, and a separate cold storage for reagent storage is also required inside the instrument to ensure the performance of the reagent. Although this bottled detection reagent makes the cost of small instruments comparable to that of high-throughput large instruments, its ease of use is far from meeting the needs of clinical laboratory personnel for speed and flexibility.

[0005] There is also a small chemiluminescence analyzer that uses a disposable test strip for testing. This test strip places the necessary reagents required for each test in the various holes of the test strip. During the test, the instrument only needs to use a disposable pipette tip to mix the sample to be tested in the test strip with each reagent. The washing liquid and waste liquid required to clean impurities can also be concentrated on the test strip. After the test is completed, the test strip is discarded. Although this test strip solution saves the cleaning liquid path, it also greatly increases the cost of a single test. Summary of the invention

[0006] In view of the technical problems mentioned above, the present invention aims to provide a photochemiluminescence analyzer, which can solve at least one of the above problems.

[0007] The present invention also provides a photoinduced chemiluminescence analysis method, which can solve at least one of the above problems.

[0008] According to the present invention, a photochemiluminescence analyzer is provided, comprising an incubation reaction module, a storage module, a pipetting arm module and a detection module;

[0009] The storage module includes a reagent storage module and a sample storage module. A reagent inlet and a sample inlet are provided on the housing of the photochemiluminescence analyzer, and the storage module can be accessed through the reagent inlet and the sample inlet.

[0010] The pipetting arm module includes a pipetting arm and a pipetting arm driving device, which is configured to inject the reagents and samples in the storage module into the incubation reaction module;

[0011] The incubation reaction module includes an incubation reaction tray, on which a placement position for storing a reaction cup is provided, and the pipetting arm injects the reagent and the sample into the reaction cup;

[0012] The detection module is arranged above the incubation reaction plate and is used to detect the detection signal emitted from the reaction cup;

[0013] The reagent storage module comprises a reagent tray, and the sample storage module comprises a sample tray. The reagent tray and the sample tray are coaxially sleeved.

[0014] In a preferred embodiment, it further comprises a tip placement module, wherein the tip placement module comprises at least two tip placement racks placed side by side, and the tip placement racks are provided with a plurality of tips;

[0015] In a preferred embodiment, a tip reusing position is provided on the tip placement rack.

[0016] In a preferred embodiment, it further comprises a reaction cup placement module, wherein the reaction cup placement module comprises at least one reaction cup rack, and a plurality of reaction cups are placed on the reaction cup rack;

[0017] The reaction cup placement module also includes a pull-out tray.

[0018] In a preferred embodiment, the photochemiluminescence analyzer further includes a first robotic arm and a transfer and mixing module, wherein the first robotic arm is configured to grab the cuvette on the cuvette rack to the transfer and mixing module, and the movement height of the first robotic arm is lower than the top surface of the tip placement module.

[0019] In a preferred embodiment, it further comprises a second robotic arm, which is rotatably disposed between the incubation reaction tray and the pipette tip placement module, and is used to grab the reaction cup on the transfer and mixing module to the incubation reaction tray.

[0020] In a preferred embodiment, the transfer mixing module includes a transfer mixing disk, which is arranged at the intersection of the horizontal strokes of the first robotic arm and the second robotic arm, and completes the mixing action through reciprocating rotation.

[0021] In a preferred embodiment, the transfer mixing plate includes a plurality of reaction cup placement positions, and the plurality of reaction cup placement positions are distributed in a circle.

[0022] In a preferred embodiment, the sample disk is configured in an annular shape and is coaxially sleeved on the outer side of the reagent disk.

[0023] According to the present invention, a photochemiluminescence analysis method is also provided, using the photochemiluminescence analyzer provided according to the present invention, comprising the following steps:

[0024] S1. Pull out the cuvette rack from the front of the instrument, place an empty cuvette in it, and push the cuvette rack into the instrument. Manually place the reagent bottle into the reagent tray from the reagent inlet, and place the sample tube to be tested into the sample tray from the sample inlet.

[0025] S2, the first robotic arm grabs the empty cuvette and moves it to the transfer mixing plate;

[0026] S3, the transfer mixing disk rotates, the second robotic arm grabs the reaction cup containing the sample and puts it into the outer ring of the incubation reaction disk, and the incubation reaction disk rotates according to a predetermined timing;

[0027] S4, the pipetting arm assembles the pipette tip in the pipette tip placement module, moves to the sample tray, and pipettes the designated sample in the sample tray and adds it into the reaction cup in the transfer mixing tray;

[0028] S5, the pipetting arm moves to the top of the waste box to discard the pipette tip, reassembles the pipette tip in the pipette tip placement module, and then moves to the reagent disk, sucks the reagent in the reagent disk and adds it into the reaction cup, and the incubation reaction disk rotates according to the predetermined sequence;

[0029] S6, the second robotic arm grabs the reaction cup containing the mixed solution and puts it into the transfer mixing plate, and the transfer mixing plate reciprocates to mix;

[0030] S7, after the mixing is completed, the second robot arm grabs the reaction cup and places it on the inner circle measuring disk of the incubation reaction disk;

[0031] S8, the detection module above the incubation reaction disk photoexcites the substance in the reaction cup and then performs detection;

[0032] S9, the second robot arm grabs the reaction cup after detection and puts it into the transfer mixing plate, and the first robot arm grabs the reaction cup and puts it into the waste box for disposal.

[0033] Compared with the prior art, the advantages of the present application are as follows.

[0034] The present invention effectively and fully utilizes the spatial travel of the pipette arm and the first robotic arm by staggering the spatial height of the pipette tip placement module and the pipette arm with the reaction cup placement module and the first robotic arm, prevents spatial interference between the two, and helps to further reduce the occupied space of the analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention will be described below with reference to the accompanying drawings.

[0036] Figure 1 A schematic diagram showing the external structure of an embodiment of a photochemiluminescence analyzer according to the present invention is shown;

[0037] Figure 2 A schematic diagram of a top view of an embodiment of a photochemiluminescence analyzer according to the present invention is shown;

[0038] Figure 3 A schematic structural diagram of an embodiment of an incubation tray according to the present invention is shown;

[0039] Figure 4 A schematic cross-sectional structure diagram of an embodiment of an incubation tray according to the present invention is shown;

[0040] Figure 5 A schematic structural diagram of an embodiment of a second mechanical arm according to the present invention is shown;

[0041] Figure 6 A schematic structural diagram of an embodiment of a pipetting arm according to the present invention is shown.

[0042] In the figure: 1, housing; 2, cuvette placement module; 3, tip placement module; 101, waste box; 102, reagent inlet; 103, sample inlet;

[0043] 4. First robotic arm; 41. First guide rail; 42. Second guide rail; 801. Gripper vertical zero position optical coupler; 802. Gripper vertical motor; 803. Gripper vertical yardstick; 804. Gripper vertical yardstick optical coupler; 805. Gripper vertical slide rail; 806. Gripper vertical belt; 807. Gripper jaw; 808. Gripper horizontal yardstick optical coupler; 809. Gripper horizontal yardstick; 810. Gripper horizontal belt; 811. Gripper horizontal slide rail; 812. Reaction cup presence detection optical coupler; 813. Gripper jaw motor;

[0044] 5. Pipette arm module; 51. Third rail; 52. Fourth rail; 501. Front and rear motors of sample loading arm; 502. Front and rear slide rails of sample loading arm; 503. Front and rear tank chains of sample loading arm; 504. Front end fixing bracket of sample loading arm; 505. Front and rear zero position optical coupler of sample loading arm; 506. Pipette syringe motor; 507. Pipette; 508. Pipette TIP head rod; 509. Front and rear rails of sample loading arm; 510. Left and right tank chains of sample loading arm; 511. Left and right slide rails of sample loading arm; 512. Vertical belt of sample loading arm; 513. Vertical motor of sample loading arm; 514. Left and right motors of sample loading arm;

[0045] 6. Transfer and mixing module;

[0046] 7. Incubation reaction module; 701. Photometric incubation tray cover; 702. Photometric optical disk; 703. Incubation reaction tray; 704. Component support column; 705. Photoexcitation component; 706. Temperature sensor; 707. Heating plate; 708. Temperature control switch; 709. Photometric optical disk motor; 710. Driving pulley; 711. Synchronous belt tensioner; 712. Incubation tray motor; 713. Optical detection component;

[0047] 714, optical detection cover; 715, optical O-type gasket; 716, filter; 717, filter pressing block; 718, reaction cup; 719, calibration O-type gasket; 720, calibration board; 721, calibration module cover; 722, calibration module shell; 723, attenuation plate; 724, attenuation plate pressing block; 725, heating plate pressing block; 726, reaction cup limit assembly; 727, plano-concave mirror pressing plate; 728, optical plano-concave mirror; 729, optical detection shell; 730, photon counter;

[0048] 8. Second robotic arm;

[0049] 9. Reagent storage module; 10. Sample storage module; 100. Photochemiluminescence analyzer.

[0050] In the present application, all drawings are schematic drawings, which are only used to illustrate the principles of the present invention and are not drawn according to the actual scale. DETAILED DESCRIPTION

[0051] The present invention will be described below with reference to the accompanying drawings.

[0052] It should be noted that the directional terms or qualifiers "upper", "lower", "front", "back", "left", "right", etc. used in this application are all relative to the referenced Figure 1 They are not intended to define the absolute positions of the components involved, but may vary depending on the specific circumstances.

[0053] Figure 1 A schematic diagram of the external structure of the photochemiluminescence analyzer 100 is shown. Figure 2 The figure shows a schematic diagram of the internal structure of the photochemiluminescence analyzer 100 from a top view.

[0054] like Figure 1 and Figure 2 As shown, the photochemiluminescence analyzer 100 includes a pipetting arm module 5, an incubation reaction module 7, a storage module and a detection module.

[0055] In a specific embodiment, the storage module includes a reagent storage module 9 for storing reagents and a sample storage module 10 for storing samples. A reagent inlet 102 and a sample inlet 103 are provided on the housing 1 of the photochemiluminescence analyzer 100, and the reagent storage module 9 and the sample storage module 10 of the storage module can be accessed through the reagent inlet 102 and the sample inlet 103, respectively.

[0056] In a preferred embodiment, the reagent storage module 9 includes a reagent tray, and the sample storage module 10 includes a sample tray, and the reagent tray and the sample tray are coaxially arranged. Figure 2 As shown, the sample disk is arranged in a ring shape and is coaxially sleeved on the outer side of the reagent disk.

[0057] The pipetting arm module 5 is located at the left rear of the housing 1, and the pipetting arm module 5 can inject the reagents and samples in the storage module into the incubation reaction module 7. Figure 2 As shown, specifically, the pipetting arm module 5 includes a third guide rail 51 and a fourth guide rail 52. The length direction of the third guide rail 51 is arranged along the front-to-back direction of the housing 1, and the length direction of the fourth guide rail 52 is arranged along the left-to-right direction of the housing 1, and the fourth guide rail 52 is movably arranged on the third guide rail 51, that is, the fourth guide rail 52 can move forward and backward in the housing 1 along the third guide rail 51. A mechanism for adapting to the tip in the tip placement module 3 is movably arranged on the fourth guide rail 52 along its length direction.

[0058] The reagent storage module 9 and the sample storage module 10 are located on the right side of the incubation reaction module 7. The left-right lengths of the reagent storage module 9, the sample storage module 10 and the incubation reaction module 7 are within the length range of the fourth guide rail 52, and the left-right lengths of the reaction cup placement module 2 and the pipette tip placement module 3 are also within the length range of the fourth guide rail 52. The front-to-back lengths of the incubation reaction module 7 and the pipette tip placement module 3, and the front-to-back lengths of the reagent storage module 9, the sample storage module 10 and the reaction cup placement module 2 are all within the length range of the third guide rail 51.

[0059] The pipetting arm module 5 includes a pipetting arm and a pipetting arm driving device. In a specific embodiment, Figure 6 As shown, the pipetting arm of the pipetting arm module 5 includes a loading arm front and rear slide rails 502, a loading arm left and right slide rails 511 and a pipette 507. The loading arm left and right slide rails 511 are movably arranged on the loading arm front and rear slide rails 502 along the length direction of the loading arm front and rear slide rails 502, and the pipette 507 is movably arranged on the loading arm left and right slide rails 511 along the length direction of the loading arm left and right slide rails 511.

[0060] The pipetting arm driving device of the pipetting arm module 5 includes a pipetting arm front and rear motor 501 arranged at the end of the pipetting arm front and rear slide rails 502, a pipetting arm front end fixed bracket 504 is arranged at the other end of the pipetting arm front and rear slide rails 502, a pipetting arm front and rear zero position optical coupler 505 is arranged on the pipetting arm front and rear fixed bracket 504, a pipetting arm front and rear tank chain 503 is arranged between the pipetting arm front and rear fixed bracket 504 and the pipetting arm front and rear motor 501, and the pipetting arm front and rear motor 501 can drive the pipetting arm left and right slide rails 511 to move along the pipetting arm front and rear slide rails 502 through the pipetting arm front and rear tank chain 503.

[0061] A left and right motor 514 is provided at one end of the left and right slide rails 511 of the sample loading arm. The left and right motor 514 is connected to the left and right tank chains 510 of the sample loading arm. The left and right motor 514 of the sample loading arm can drive the pipette 507 to move along the left and right slide rails 511 of the sample loading arm through the left and right tank chains 510 of the sample loading arm.

[0062] A front and rear guide rail 509 is provided at the other end of the left and right slide rails 511 of the loading arm. The front and rear guide rail 509 is parallel to the front and rear slide rails 502 of the loading arm. The front and rear guide rail 509 of the loading arm can guide the movement of the left and right slide rails 511 of the loading arm.

[0063] The pipette 507 includes a sample loading arm vertical motor 513 arranged at the top, and the sample loading arm vertical motor 513 is connected to the sample loading arm vertical belt 512. The pipette 507 also includes a pipette syringe motor 506 and a pipette TIP top rod 508. The pipette syringe motor 506 is located above the pipette TIP top rod 508. The sample loading arm vertical motor 513 drives the pipette syringe motor 506 to move up and down through the sample loading arm vertical belt 512. The pipette syringe motor 506 can drive the pipette TIP top rod 508 to work.

[0064] In a specific embodiment, Figure 3 As shown, the incubation reaction module 7 includes an incubation reaction disk 703, on which a placement position for storing reaction cups is provided, and the pipetting arm module 5 can inject the reagents and samples in the storage module into the reaction cups in the incubation reaction disk 703. A plurality of component support columns 704 are evenly arranged along the circumferential direction at the lower end of the incubation reaction disk 703, and the component support columns 704 support the incubation reaction disk 703. A photometric incubation disk cover 701 is provided at the upper end of the incubation reaction disk 703, and the photometric incubation disk cover 701 shields the upper end of the incubation reaction disk 703. A measuring optical disc 702 is provided inside the incubation reaction disk 703, and the measuring optical disc 702 is coaxially rotatably arranged in the incubation reaction disk 703. A light excitation component 705 and an optical detection component 713 for detecting the reaction cup 718 are fixedly arranged on the photometric incubation disk cover 701. A temperature sensor 706 is provided on the side of the incubation reaction tray 703, a heating plate 707 is provided at the bottom of the incubation reaction tray 703, and a temperature control switch 708 is electrically connected to the heating plate 707. The temperature of the heating plate 707 can be controlled by the temperature control switch 708, thereby controlling the temperature of the reaction cup 718. Two synchronous belt tensioners 711 are provided on the outside of the incubation reaction tray 703, and a measuring disc motor 709 and an incubation disc motor 712 are provided on the two synchronous belt tensioners 711, respectively. The measuring disc motor 709 and the incubation disc motor 712 are connected to the measuring disc 702 and the incubation reaction module 7 through two driving pulleys 710.

[0065] The detection module includes an optical detection component 713, and the detection device is arranged above the incubation reaction disk 703, and is used to detect the detection signal emitted from the reaction cup in the incubation reaction disk 703. In a specific embodiment, Figure 4As shown, the optical detection assembly 713 includes an optical detection shell 729 fixedly arranged on the upper part of the photometric incubation tray cover 701, and the optical detection shell 729 is sealed and connected to the photometric incubation tray cover 701. An optical detection cover plate 714 is fixedly arranged at the upper end of the optical detection shell 729 to play a light shielding role. A photon counter 730 is arranged inside the optical detection shell 729, and an optical concave mirror 728 and a filter 716 are arranged in sequence from top to bottom at the lower end of the optical detection shell 729, and light can pass through the optical concave mirror 728 and the filter 716 through the photometric incubation tray cover 701 to illuminate the reaction cup 718 in the incubation reaction module 7. Furthermore, the optical plano-concave mirror 728 is fixedly set at the bottom of the optical detection shell 729 through the plano-concave mirror pressing plate 727, the filter 716 is fixedly set in the photometric incubation tray cover 701 through the filter pressing block 717, and an optical O-ring 715 is arranged between the optical plano-concave mirror 728 and the filter 716.

[0066] In a specific embodiment, a cuvette stopper assembly 726 for placing a cuvette 718 is provided on the measuring disc 702. The heating plate 707 is fixed to the bottom of the incubation reaction disc 703 by a heating plate pressing block 725. Specifically, a groove for installing the heating plate 707 is provided at the bottom of the incubation reaction disc 703. The heating plate pressing block 725 is provided at the lower part of the heating plate 707. The heating plate pressing block 725 is fixedly connected to the incubation reaction disc 703.

[0067] A calibration module is provided at the bottom of the incubation reaction disk 703, and the position of the calibration module corresponds to the optical detection assembly 713. The calibration module includes a calibration module shell 722 fixedly provided at the bottom of the incubation reaction disk 703, a cavity is provided inside the calibration module shell 722, a calibration board 720 and a calibration module cover 721 are provided at the bottom of the calibration module shell 722, and the calibration board 720 and the calibration module cover 721 jointly close the calibration module shell 722. A light source is provided on the calibration board 720, and an attenuation sheet 723 is provided at the top of the calibration module shell 722, and the light source on the calibration board 720 can be irradiated to the optical detection assembly 713 through the attenuation sheet 723. Further, the attenuation sheet 723 is fixed to the top of the calibration module shell 722 by an attenuation sheet pressing block 724, and a calibration O-type gasket 719 is provided between the lower end of the attenuation sheet 723 and the calibration module shell 722.

[0068] A second robotic arm 8 is disposed between the incubation reaction module 7 and the transfer mixing module 6, and the second robotic arm 8 is configured to move the reaction cup 718 of the transfer mixing module 6 into the incubation reaction module 7. Specifically, the second robotic arm 8 in this embodiment is rotatably disposed in the housing 1, and the end of the second robotic arm 8 can pass through the incubation reaction module 7 and the transfer mixing module 6 during the rotation process.

[0069] During operation, a plurality of empty cuvettes 718 are placed in the cuvette placement module 2, and a plurality of pipette tips are placed in the pipette tip placement module 3. The first robot arm 4 places the cuvette 718 in the cuvette placement module 2 into the transfer and mixing module 6; the pipette arm module 5 moves to the pipette tip placement module 3, replaces a new pipette tip, and adds the sample in the sample storage module 10 into the cuvette 718 in the transfer and mixing module 6; the pipette arm module 5 moves to the pipette tip placement module 3, replaces a new pipette tip, and adds the reagent in the reagent storage module 9 into the cuvette 718 in the transfer and mixing module 6 to mix the reagent and the sample; the second robot arm 8 places the cuvette 718 in the transfer and mixing module 6 into the incubation reaction module 7.

[0070] The photochemiluminescence analyzer 100 further includes a cuvette placement module 2 and a tip placement module 3. The incubation reaction module 7 is disposed behind the tip placement module 3, the reagent storage module 9 is disposed behind the cuvette placement module 2, and the sample storage module 10 is disposed behind the cuvette placement module 2.

[0071] The reaction cup placement module 2 and the pipette tip placement module 3 are both arranged at the front side of the photochemiluminescence analyzer 100. Figure 1 As shown, the upper end surface of the reaction cup placement module 2 is not higher than the lower end surface of the pipette tip placement module 3, that is, the upper end surface of the reaction cup placement module 2 is parallel to the lower end surface of the pipette tip placement module 3, or the upper end surface of the reaction cup placement module 2 is lower than the lower end surface of the pipette tip placement module 3.

[0072] like Figure 2 As shown, in this embodiment, from the top view, the reaction cup placement module 2 is located on the right side of the pipette tip placement module 3, and the reaction cup placement module and the pipette tip placement module 3 are both located on the front side of the housing 1 (i.e. Figure 2 ).

[0073] The tip placement module 3 includes at least two tip placement racks placed side by side, and a plurality of tips are placed on the tip placement racks. A tip reuse position is provided on the tip placement rack. When it is necessary to replace the tip by sucking different liquids, the tip can be temporarily placed in the tip reuse position to meet the tip reuse requirements and save tips.

[0074] The reaction cup placement module 2 includes at least one reaction cup rack, and the reaction cup rack can place multiple reaction cups. Preferably, the reaction cup placement module also includes a removable tray. In a preferred embodiment, the tip placement module 3 and the reaction cup placement module 2 are movably arranged on the casing 1. During operation, the tip placement module 3 and the reaction cup placement module 2 are located inside the casing 1. When it is necessary to replace or add a tip or a reaction cup 718, the tip placement module 3 and the reaction cup placement module 2 can be pulled out from the front side of the casing 1 by pulling. In a specific embodiment, the tip placement module 3 and the reaction cup placement module 2 can be set to a drawer-like structure.

[0075] The first robot arm 4 is configured to grab the cuvette on the cuvette rack of the cuvette placement module 2 and place it in the transfer and mixing module 6 . The movement height of the first robot arm 4 is lower than the top surface of the pipette tip placement module 3 .

[0076] In this embodiment, the first robot arm 4 is located at the front right of the housing 1, and the first robot arm 4 is configured to grab the cuvette 718 in the cuvette placement module 2. Specifically, the first robot arm 4 includes a first guide rail 41 and a second guide rail 42, the length direction of the first guide rail 41 is arranged along the front-to-back direction of the housing 1, the length direction of the second guide rail 42 is arranged along the left-to-right direction of the housing 1, and the second guide rail 42 is movably arranged on the first guide rail 41, that is, the second guide rail 42 can move forward and backward in the housing 1 along the first guide rail 41. A gripper for grabbing the cuvette 718 is movably arranged on the second guide rail 42 along its length direction.

[0077] In a specific embodiment, Figure 5 As shown, the first mechanical arm 4 includes a gripper vertical slide rail 805 and a gripper horizontal slide rail 811. The gripper vertical slide rail 805 is arranged in the vertical direction, and the gripper horizontal slide rail 811 is arranged in the horizontal direction. The gripper vertical slide rail 805 is movably arranged on the gripper horizontal slide rail 811 along the length direction of the gripper horizontal slide rail 811. A gripper vertical zero position optical coupler 801 is arranged on the upper part of the side of the gripper vertical slide rail 805, and a gripper vertical motor 802 is arranged on the side of the gripper vertical slide rail 805 away from the gripper horizontal slide rail 811. The gripper vertical motor 802 is connected to the gripper vertical belt 806. A gripper vertical yardstick optical coupler 804 is also arranged on the side of the gripper vertical slide rail 805, and the gripper vertical yardstick optical coupler 804 is located below the gripper vertical zero position optical coupler 801.

[0078] A gripper clamp motor 813 is movably arranged on the gripper vertical slide rail 805 in the vertical direction, and the gripper vertical motor 802 drives the gripper clamp motor 813 to move up and down through the gripper vertical belt 806. The gripper clamp motor 813 is connected to the gripper clamp 807, and the gripper clamp motor 813 can drive the gripper clamp 807 to grab the reaction cup 718. A gripper vertical yardstick 803 adapted to the gripper vertical zero position optical coupler 801 and the gripper vertical yardstick optical coupler 804 is arranged on the gripper clamp motor 813, and the gripper vertical yardstick 803 can move with the gripper clamp motor 813. A reaction cup presence detection optical coupler 812 is arranged above the gripper clamp 807.

[0079] A gripper horizontal belt 810 is provided below the gripper horizontal slide rail 811, and the gripper horizontal belt 810 can drive the gripper vertical slide rail 805 to move left and right. A gripper horizontal yardstick optical coupler 808 and a gripper horizontal yardstick 809 are provided on the gripper horizontal slide rail 811.

[0080] In the working state, the first robot arm 4 and the pipetting arm module 5 are staggered in height to avoid interference. That is, from the height direction, the pipetting arm module 5 is located above the tip placement module 3, and the first robot arm 4 is located between the pipetting arm module 5 and the reaction cup placement module 2.

[0081] According to the present invention, the photochemiluminescence analyzer 100 further includes a transfer and mixing module 6 , which is disposed at the rear between the tip placement module 3 and the cuvette placement module 2 .

[0082] The front-to-back lengths of the transfer and mixing module 6 and the reaction cup placement module 2 are both within the length range of the first guide rail 41, and the left-to-right lengths of the transfer and mixing module 6 and the reaction cup placement module 2 are both within the length range of the second guide rail 42, so that the first robot arm 4 can place the reaction cup 718 in the reaction cup placement module 2 into the transfer and mixing module 6.

[0083] According to the present invention, the photochemiluminescence analyzer 100 also includes a second robotic arm 8, which is rotatably disposed between the incubation reaction module 7 and the tip placement module 3, and is used to grab the reaction cup on the transfer mixing module 6 to the incubation reaction module 7.

[0084] The transfer mixing module 6 includes a transfer mixing disk, which is arranged at the intersection of the horizontal strokes of the first robotic arm 4 and the second robotic arm 8, and completes the mixing action through reciprocating rotation.

[0085] The various parts of the present invention are compactly distributed, and the tip placement module 3, the pipette arm module 5, the reaction cup placement module 2, and the first robotic arm 4 are staggered in space, which effectively makes full use of the spatial travel of the pipette arm module 5 and the first robotic arm 4 to prevent spatial interference between the two, thereby allowing the various parts of the photochemiluminescence analyzer 100 to be more compactly distributed.

[0086] In a preferred embodiment, the reagent storage module 9 is used to place reagent bottles (not shown in the figure), and a scanner (not shown in the figure) is arranged on the reagent storage module 9, and the scanner is used to detect the barcode on the reagent bottle.

[0087] According to the present invention, in a preferred embodiment, the reagent disk of the reagent storage module 9 and the sample disk of the sample storage module 10 are rotatably arranged in the housing 1, and the reagent disk and the sample disk are designed as a multi-ring structure, and a central reagent disk and an annular sample disk are sequentially arranged radially from the inside to the outside around the rotation axis. The reagent storage module 9 is used to store the reagents required for the test, and the sample storage module 10 is used to store the samples to be tested.

[0088] Bottled reagents are stored in the reagent storage module 9, and each bottle of reagent carries the reagent amount required for dozens of detections. The reagent bottle can be fixed in the reagent tank at the bottom of the reagent storage module 9. The shape of the reagent tank matches the bottom of the reagent bottle, so that the reagent bottle is engaged in the reagent tank. The reagent storage module 9 is provided with a separate opening on the casing 1 of the instrument (i.e., the photoluminescence analyzer 100), and the user can put the reagent into the reagent tank of the reagent storage module 9 through the opening. A scanner opening is provided on the peripheral wall of the reagent storage module 9, and the light beam of the scanner is shot on the reagent bottle from the opening, so that the scanner can detect the bar code on the reagent bottle and record the information of the bar code in the database of the instrument. The bottom of the reagent storage module 9 is provided with a cooling element, i.e., a Peltier element, on the side opposite to the reagent tank. It provides the necessary refrigeration temperature for the reagent storage module 9 to preserve the reagent. The cooling element itself is cooled by the air duct.

[0089] The sample storage module 10 is provided with a plurality of test tube slots along the circumferential direction. The test tube slot has a fan-shaped back plate extending from top to bottom along the height direction of the sample storage module 10 and matching the shape of the test tube, and has a plurality of positioning rings protruding from the back plate. The sample tube can be fixed in the positioning ring. The positioning ring may not be completely closed, and two positioning rings may be provided at the top and the bottom along the height direction. The sample storage module 10 is provided with a separate opening on the housing 1 of the instrument, through which the user can place the sample to be tested into the test tube slot.

[0090] The incubation reaction module 7 is arranged beside the reagent storage module 9 and the sample storage module 10. There are a plurality of vacancies on the incubation reaction module 7 to accommodate the reaction cup 718, and a heating film is attached under the incubation plate. The heating film is used to provide the sample and reagent mixture in the reaction cup 718 with the required temperature for the reaction.

[0091] The tip placement module 3 and the reaction cup placement module 2 are arranged side by side at the front side of the instrument and can be designed as a push-pull drawer. The user can put in and take out the tip tray or the reaction cup 718 tray by pushing and pulling.

[0092] The upper edge of the reaction cup placement module 2 is lower than the lower edge of the pipette tip placement module 3. The first robot arm 4 transfers the reaction cup 718 from the reaction cup placement module 2 to the intermediate transfer and mixing module 6. The first robot arm 4 is slightly higher than the pipette tip placement module 3, but much lower than the height of the pipetting arm module 5.

[0093] According to the present invention, a photo-induced chemiluminescence analysis method is also provided, comprising the following steps: S1, extracting a reaction cup rack from the front of the instrument, placing an empty reaction cup, and pushing the reaction cup rack into the instrument, manually placing a reagent bottle into the reagent tray from the reagent inlet 102, and placing a sample tube to be tested into the sample tray from the sample inlet 103; S2, the first robotic arm 4 grabs the empty reaction cup 718 and moves it into the transfer mixing tray of the transfer mixing module 6; S3, the transfer mixing tray rotates, and the second robotic arm 8 grabs the reaction cup 718 containing the sample and places it into the outer ring of the incubation reaction tray 703, and the incubation reaction tray 703 is heated according to the existing The pipette arm module 5 is rotated according to the timing sequence; S4, the pipette arm module 5 assembles the pipette tip in the pipette tip placement module 3, and moves to the sample disk, sucks the specified sample in the sample disk and adds it to the reaction cup 718 in the transfer mixing disk; S5, the pipette arm module 5 moves to the top of the waste box 101 to discard the pipette tip, reassembles the pipette tip in the pipette tip placement module 3, and moves to the reagent disk, sucks the reagent in the reagent disk and adds it to the reaction cup of the incubation reaction disk 703, and the incubation reaction disk 703 rotates according to the established timing sequence; S6, the second robot arm 8 grabs the reaction cup 718 containing the mixed solution and puts it into the transfer mixing disk, and the transfer mixing disk rotates back and forth to mix;

[0094] S7. After mixing is completed, the second robot arm 8 grabs the reaction cup in the transfer mixing plate and places it on the inner circle measuring optical disc 702 of the incubation reaction plate 703; S8. The detection device above the incubation reaction plate 703 optically excites the material in the reaction cup 718 and then performs detection; S9. The second robot arm 8 grabs the detected reaction cup 718 and places it into the transfer mixing plate, and the first robot arm 4 grabs the reaction cup 718 in the transfer mixing plate and discards it in the waste box 101.

[0095] Combined with Figure 1 and Figure 2As shown, the photochemiluminescence analysis method specifically includes the following steps. The reagent is placed in the reagent storage module 9 through the reagent inlet 102 above the reagent storage module 9, and the sample is placed in the sample storage module 10 through the sample inlet 103 above the sample storage module 10. After that, the instrument obtains the test item corresponding to the sample and determines the position of the detection reagent required for the test item. The empty reaction cup 718 is placed in the transfer mixing module 6 from the reaction cup placement module 2 by the first mechanical arm 4. The pipetting arm module 5 then picks up a disposable pipette tip, sucks the sample and dispenses the sample into the empty reaction cup 718. The pipette tip is then discarded, and a new disposable pipette tip is picked up again to suck the reagent required for the test and dispense the reagent into the reaction cup 718 that already contains the sample. Depending on the requirements of the required detection reagent, 2 to 3 disposable pipette tips may be required during the reagent dispensing process. That is, a new disposable pipette tip is replaced after sucking and dispensing a kit component. The photochemiluminescence kit generally includes one or two reagents containing luminescent particles. These kits are also equipped with reagents containing photosensitive particles.

[0096] After all the required reagents have been dispensed into the reaction cup 718, the mixture of the sample and the reagent is mixed in the transfer mixing module 6. The mixed reaction cup 718 is rotated by the second robot 8 to the incubation reaction module 7, and incubated in the incubation reaction module 7 for a period of time, so that the temperature of the mixture can be incubated to the required temperature.

[0097] Subsequently, the incubation reaction module 7 rotates to transfer the reaction cup 718 to the bottom of the optical detection component 713. The optical detection component 713 sends a laser to the mixture to stimulate the luminescent reaction in the mixture. Specifically, the substance to be tested in the sample is first combined with the luminescent particles in the luminescent particle reagent. After the photosensitive particle reagent is added, the luminescent particles in the previous step of the conjugate are combined with the photosensitive particles. After receiving the laser from the laser, light of a specific wavelength is emitted. The emitted light is collected by the optical device of the optical detection component 713 and converted into concentration data of the substance to be tested.

[0098] The optical detection assembly 713 is disposed above the incubation reaction module 7 and corresponds to one of the reaction cups 718. When performing excitation and detection, the optical detection assembly 713 is lowered above the reaction cup 718, so that the optical detection assembly 713 and the reaction cup 718 form a relatively more light-tight environment. After the detection is completed, the optical detection assembly 713 returns to the ready position again.

[0099] After all the processes are finished, the reaction cup 718 is moved to the discarded cup position and the discarded reaction cup 718 is moved to the waste box 101. The user can replace the waste box 101 regularly.

[0100] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0101] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0102] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0103] Finally, it should be noted that the above is only a preferred embodiment of the present invention and does not constitute any limitation to the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments or to replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A photochemiluminescence analyzer, characterized in that: It comprises an incubation reaction module (7), a storage module, a pipetting arm module (5) and a detection module; The storage module comprises a reagent storage module (9) and a sample storage module (10). A reagent inlet and a sample inlet are provided on the housing of the photochemiluminescence analyzer, and the storage module can be accessed through the reagent inlet and the sample inlet. The pipetting arm module (5) comprises a pipetting arm and a pipetting arm driving device, which is configured to inject the reagents and samples in the storage module into the incubation reaction module; The incubation reaction module (7) comprises an incubation reaction tray, on which a placement position for storing a reaction cup is provided, and the pipetting arm injects the reagent and the sample into the reaction cup; The detection module is arranged above the incubation reaction plate and is used to detect the detection signal emitted from the reaction cup; The reagent storage module (9) comprises a reagent tray, and the sample storage module (10) comprises a sample tray, and the reagent tray and the sample tray are coaxially sleeved.

2. A photochemiluminescence analyzer according to claim 1, characterized in that: It also comprises a pipette tip placement module (3), wherein the pipette tip placement module (3) comprises at least two pipette tip placement racks placed side by side, and the pipette tip placement racks are provided with a plurality of pipette tips.

3. A photochemiluminescence analyzer according to claim 2, characterized in that: The suction tip placement rack is provided with a suction tip reuse position.

4. A photochemiluminescence analyzer according to claim 3, characterized in that: It also comprises a reaction cup placement module (2), wherein the reaction cup placement module (2) comprises at least one reaction cup rack, and a plurality of reaction cups are placed on the reaction cup rack; The reaction cup placement module also includes a pull-out tray.

5. A photochemiluminescence analyzer according to claim 4, characterized in that: The photochemiluminescence analyzer further comprises a first robotic arm (4) and a transfer and mixing module (6), wherein the first robotic arm is configured to grab a cuvette on a cuvette rack and place it in the transfer and mixing module (6), and the movement height of the first robotic arm is lower than the top surface of the pipette tip placement module.

6. A photochemiluminescence analyzer according to claim 5, characterized in that: It also includes a second robotic arm (8), which is rotatably arranged between the incubation reaction tray and the pipette tip placement module, and is used to grab the reaction cup on the transfer mixing module to the incubation reaction tray.

7. A photochemiluminescence analyzer according to claim 6, characterized in that: The transfer mixing module (6) comprises a transfer mixing disk, which is arranged at the intersection of the horizontal travels of the first mechanical arm and the second mechanical arm and performs a mixing action through reciprocating rotation.

8. A photochemiluminescence analyzer according to claim 7, characterized in that: The transfer mixing plate includes a plurality of reaction cup placement positions, and the plurality of reaction cup placement positions are distributed in a circle.

9. The photochemiluminescence analyzer according to claim 1, characterized in that: The sample disk is arranged in a ring shape and is coaxially sleeved on the outer side of the reagent disk.

10. A photochemiluminescence analysis method, characterized in that: Using the photochemiluminescence analyzer according to any one of claims 1 to 9 comprises the following steps: S1. Pull out the cuvette rack from the front of the instrument, place an empty cuvette in it, and push the cuvette rack into the instrument. Manually place the reagent bottle into the reagent tray from the reagent inlet, and place the sample tube to be tested into the sample tray from the sample inlet. S2, the first robot arm (4) grabs the empty reaction cup and moves it to the transfer mixing plate; S3, the transfer mixing disk rotates, the second robot arm (8) grabs the reaction cup containing the sample and puts it into the outer ring of the incubation reaction disk, and the incubation reaction disk rotates according to a predetermined sequence; S4, the pipetting arm assembles the pipette tip in the pipette tip placement module (3), moves to the sample tray, and pipettes the designated sample in the sample tray and adds it to the reaction cup in the transfer mixing tray; S5, the pipetting arm moves to the waste box (101) to discard the pipette tip, reassembles the pipette tip in the pipette tip placement module (3), and then moves to the reagent disk, sucks the reagent in the reagent disk and adds it to the reaction cup, and incubates the reaction disk to rotate according to a predetermined sequence; S6, the second robot arm (8) grabs the reaction cup containing the mixed solution and places it into the transfer mixing plate, and the transfer mixing plate reciprocates to mix the solution; S7, after the mixing is completed, the second robot arm (8) grabs the reaction cup and places it on the inner circle measuring disk of the incubation reaction disk; S8, the detection module above the incubation reaction disk photoexcites the substance in the reaction cup and then performs detection; S9, the second robot arm (8) grabs the reaction cup after detection and puts it into the transfer mixing plate, and the first robot arm (4) grabs the reaction cup and puts it into the waste box (101) for disposal.

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