Method for simultaneously identifying schisandra propinqua medicinal material and preparation of schisandra propinqua medicinal material

Abstract

The invention discloses a method for simultaneously identifying an ipomoea batatas medicinal material and a preparation thereof, and relates to the technical field of quality control of traditional Chinese medicines. The thin-layer identification method comprises the following steps: (1) taking the traditional Chinese medicine preparation containing the schisandra propinqua as a to-be-detected sample, adding an ethanol solution, performing ultrasonic treatment, filtering, evaporating filtrate to dryness, dissolving residues in an acidic aqueous solution, extracting with petroleum ether, removing a petroleum ether layer, adjusting the pH value of an acid aqueous solution, filtering, extracting filtrate with ethyl acetate, merging ethyl acetate layers, evaporating to dryness, and concentrating to obtain a sample; adding methanol into the residues for dissolving to obtain a test solution; (2) preparing an ipomoea batatas medicinal material solution, a reference medicinal material solution and an ipomoea batatas negative sample solution; and (3) respectively dripping the four solutions on a thin-layer plate, developing with a developing solvent, drying and inspecting. The thin-layer identification method disclosed by the invention not only can identify a single medicinal material of the ipomoea batatas, but also can better identify the Chinese patent medicine compound preparation, is good in component separation degree, free from interference and short in identification time, and reduces the production and inspection cost.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine quality control, and in particular to a method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof. Background Art

[0002] The main components of Schisandra chinensis are flavonoids, triferric saponins, organic acids, alkaloids, lactones, volatile oils and sugars, etc., which have the effects of removing rheumatism, reducing swelling and relieving pain, and treating traumatic injuries. Regarding the thin layer identification method of Schisandra chinensis and its traditional Chinese medicine preparation, the prior art is disclosed as follows:

[0003] The article "Liao Jingni et al., Study on the quality standard of Yao medicine Schisandrae longifolia[J]. Chinese Journal of Traditional Chinese Medicine. 2017, 32(06)" discloses a thin layer chromatography identification method for Yao medicine Schisandrae longifolia, in which the thin layer chromatography identification steps are as follows: take 1g of each powder of the product, add 45mL of ethyl acetate, ultrasonicate for 30min, filter, concentrate the filtrate to dryness, add 1mL of ethyl acetate to dissolve, and use it as the test solution. Take another 1g of the reference medicinal material and prepare the reference medicinal material solution in the same way. Take the Schisandrae chinensis alcohol B reference standard and add methanol to prepare a solution containing 1mg per 1mL, which is used as the reference solution. Take 6μL of the above test solution, reference medicinal material solution and 4μL of the reference solution, and spot them on the same silica gel GF254 thin layer plate, use cyclohexane-ethyl acetate (3:1) as the developing solvent, develop, take out, dry, and inspect under ultraviolet light (254nm). However, the spots of this method are not clear when identifying preparations, that is, it can only be used to identify medicinal materials, not preparations.

[0004] The article "Rao Weiyuan et al., Research on improving the quality standard of Wusong swelling and pain tincture [J]. Journal of Traditional Chinese Medicine and Pharmacy. 2013, 19 (12)" discloses a thin layer chromatography identification method for Schisandra chinensis in Wusong swelling and pain tincture, wherein the thin layer chromatography identification steps are as follows: take 30 mL of the product, evaporate to dryness, add 10 mL of chloroform-methanol (8:2) to dissolve the residue, filter, evaporate the filtrate to dryness, add 1 mL of methanol to dissolve the residue, and use it as the test solution. Take another 30 mL of negative control sample without Schisandra chinensis medicinal materials, and prepare a negative control solution in the same way. Take 0.5 g of Schisandra chinensis control medicinal materials, add 10 mL of chloroform-methanol (8:2) and ultrasonically treat for 20 minutes, filter, evaporate the filtrate to dryness, and add 1 mL of methanol to dissolve the residue, and use it as the control medicinal material solution. 10 μL of each of the above three solutions were taken and spotted on the same silica gel G thin layer plate with sodium carboxymethyl cellulose as the adhesive, and toluene-ethyl acetate-formic acid (8:1.5:0.5) was used as the developing agent. The plate was developed, taken out, dried, sprayed with 2% ferric chloride ethanol solution, and heated at 105°C until the spots were clearly colored. However, this method could not be reproduced in the preparation of the present invention, and the spots were unclear and had tails.

[0005] The article "Jiang Liqun, Research on the Quality Standard of Zhongfeng Tongluo Tablets [D]. Guangxi University of Chinese Medicine. 2017" discloses a thin layer chromatography identification method for Schisandrae Chinensis in Zhongfeng Tongluo Tablets, in which the thin layer chromatography identification steps are as follows: Take 20 tablets of this product, grind them into powder, take 0.3g of fine powder, put it in a conical flask, add 50ml of methanol, ultrasonic (power 90W, frequency 40kHz) for 25 minutes, leave it for 12 hours, take 20ml of supernatant, add 3 times the amount of ether and leave it for 1 hour, pour out the supernatant, add 50ml of 10% hydrochloric acid solution to the precipitate, reflux in a boiling water bath for 1 hour, filter, dissolve the precipitate with 10ml of methanol, and concentrate the methanol solution to 1ml as the test solution. Take about 2g of Schisandrae Chinensis control medicinal material, and prepare the control medicinal material solution according to the test solution preparation method. Prepare a negative sample of Schisandrae Chinensis according to the process method, take 20 tablets of negative sample, and prepare a negative control solution according to the test solution preparation method. According to the thin layer chromatography test, 10 μl of the test solution, negative control solution, and 5 μl of the control medicinal material solution were taken and spotted on the same silica gel G thin layer plate, and n-hexane-chloroform-acetone-acetic acid (0.2:8:4:0.5) was used as the developing agent. After development, the plate was taken out, dried, sprayed with 8% vanillin anhydrous ethanol-sulfuric acid solution (7-10), heated at 105°C until the spots were clearly colored, and then placed under sunlight for inspection. However, this method is time-consuming (the preparation of the test solution takes more than 12 hours), and it cannot be reproduced in the preparation of the present invention, and the spots are unclear and have tails.

[0006] The above-mentioned thin layer identification of Schisandra chinensis medicinal materials and their preparations still has certain limitations, such as unclear spots, tailing, interference with negative samples, irreproducibility, and long time consumption, which is not conducive to application in production practice. Summary of the invention

[0007] In view of the above shortcomings, the present invention provides a method for simultaneously identifying Schisandrae Chinensis medicinal materials and their preparations, which solves the problems of unclear spots, tailing, interference of negative samples, non-reproducibility, and long time consumption during thin layer identification. The thin layer identification method of the present invention can not only identify single Schisandrae Chinensis medicinal materials, but also can better identify compound preparations of Chinese patent medicines. The separation degree of each component is good, there is no interference, and it can effectively shorten the identification time and reduce the production inspection cost. The specific technical scheme is as follows:

[0008] A method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof, comprising the following steps:

[0009] (1) Preparation of test solution: Take a Chinese medicine preparation containing Schisandrae Chinensis as a test sample, add ethanol solution, ultrasonically treat, filter, evaporate the filtrate to dryness, dissolve the residue in acidic aqueous solution, extract with petroleum ether, discard the petroleum ether layer, adjust the pH of the acidic aqueous layer, filter, extract the filtrate with ethyl acetate, combine the ethyl acetate layers, evaporate to dryness, and dissolve the residue in methanol to obtain a test solution;

[0010] (2) Preparation of medicinal material solution: Take Schisandra chinensis medicinal material to prepare medicinal material solution;

[0011] (3) Preparation of control medicinal material solution: Take Schisandra chinensis control medicinal material and prepare it according to the medicinal material solution preparation method;

[0012] (4) Preparation of negative sample solution of Schisandrae chinensis deficiency: Take Schisandrae chinensis deficiency preparation as the sample to be tested and prepare it according to the method of the test solution;

[0013] (5) The test sample solution, medicinal material solution, control medicinal material solution, and Schisandrae Chinensis negative sample solution are respectively spotted on a thin layer plate, developed with a developing agent, dried, developed, and inspected.

[0014] Preferably, in step (1), the Chinese medicinal preparation containing Schisandra schizonepeta is Dieda Gushangling tincture.

[0015] Preferably, in step (1), the mass concentration of the ethanol solution is 50%-80%.

[0016] Preferably, in step (1), the ultrasonic power is 300-320W, and the ultrasonic time is 20-40min.

[0017] Preferably, in step (1), the pH of the acidic aqueous solution is 1-4.

[0018] Preferably, in step (1), the boiling range of the petroleum ether is 30-60°C.

[0019] Preferably, in step (1), the pH of the acid water layer is adjusted to 7-8.

[0020] Preferably, in step (1), the filtrate is evaporated to dryness for 1-2 h; the petroleum ether extraction is for 20-40 min; the ethyl acetate extraction is for 20-40 min; and the ethyl acetate layers are combined and evaporated to dryness for 20-40 min.

[0021] Preferably, in step (2), the method for preparing the medicinal material solution is: taking Schisandra schisandrae medicinal material, adding methanol, ultrasonically treating, filtering, evaporating the filtrate to dryness, and dissolving it with methanol to obtain the solution.

[0022] Preferably, in step (5), the developing agent is cyclohexane-ethyl acetate (3-5:2), and the inspection condition is 254 nm ultraviolet light.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention improves the processing method of the sample to be tested, and forms a new thin layer identification method together with a thin layer plate, a developing agent (cyclohexane-ethyl acetate), and 254nm ultraviolet light, which can not only identify the single medicinal material of Schisandra chinensis, but also better identify the compound preparation of Schisandra chinensis Chinese patent medicine, with good separation of each component and no interference. Compared with the traditional identification method, the overall identification of the present invention takes 5-6 hours (the traditional time is about 20 hours), which effectively shortens the identification time and reduces the production inspection cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for describing the embodiments are briefly introduced below.

[0026] Figure 1 The thin layer spectrum of Schisandra schizonepeta in Example 1;

[0027] Figure 2 The thin layer atlas of Schisandra schizonepeta in Example 2;

[0028] Figure 3 The thin layer spectrum of Schisandra schizonepeta in Example 3;

[0029] Figure 4 This is the TLC spectrum of Schisandra schizonepeta in Comparative Example 1;

[0030] Figure 5 This is the thin layer spectrum of Schisandra schisandrae in comparative example 2. DETAILED DESCRIPTION

[0031] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0032] Example 1

[0033] (1) Preparation of test solution: Take 3 g of fine powder of the test sample Dieda Guaishangling tincture (the raw material ratio of Dieda Guaishangling tincture: 17.7% of Potiancai, 10.6% of Chuifengsan, 15.9% of Schisandra chinensis, 5.3% of Guizhi, 13.3% of Liangmianzhen, 2.7% of Shengkuowu, 17.7% of Liaogewang, 3.5% of Jiulongchuan, and 13.3% of Dafengai), add 30 ml of 70% ethanol, ultrasonically treat at 320W for 20 min, filter, and evaporate the filtrate. Dry for 1.5 hours, dissolve the residue in 20 mL of aqueous solution with a pH value of 2, filter, extract twice with petroleum ether (30-60°C), 20 ml / time, each extraction for 20 minutes, discard the petroleum ether layer, adjust the acid water layer to pH 7 with sodium hydroxide solution, filter, extract the filtrate twice with ethyl acetate, 20 ml / time, each extraction for 10 minutes, combine the ethyl acetate layers, evaporate to dryness for 30 minutes, dissolve the residue in 1 mL of methanol, and use it as the test solution.

[0034] (2) Preparation of medicinal material solution and control medicinal material solution: Take 0.5 g of Schisandra chinensis medicinal material and control medicinal material, add 20 ml of methanol respectively, treat with ultrasound at 320W for 30 min, filter, evaporate the filtrate to dryness, dissolve methanol to 1 ml, and prepare medicinal material solution and control medicinal material solution.

[0035] (3) Preparation of negative sample solution lacking Schisandra chinensis: Take 2.5 g of negative sample lacking Schisandra chinensis (17.7% of Potiancai; 10.6% of Chuifengsan; 5.3% of Guizhi; 13.3% of Liangmianzhen; 2.7% of Shengkuowu; 17.7% of Liaogewang; 3.5% of Jiulongchuan; 13.3% of Dafengai) and prepare the negative sample solution lacking Schisandra chinensis according to the sample preparation method.

[0036] Thin layer chromatography conditions: 10 μL of the test solution, medicinal material solution, control medicinal material solution, and Schisandrae Chinensis negative sample solution were respectively spotted on the same silica gel GF254 thin layer plate, and cyclohexane-ethyl acetate (3:2) was used as the developing solvent. The plate was developed, taken out, dried, and examined under ultraviolet light (254 nm).

[0037] This example takes 5.8 hours to complete. The thin layer spectrum is as follows Figure 1 As shown in the figure, the test sample chromatogram shows spots of the same color at the corresponding positions of the control medicinal material chromatogram. The spots are well separated, do not interfere with each other, have no tailing phenomenon, and have no negative interference.

[0038] Example 2

[0039] (1) Preparation of test solution: Take 3 g of fine powder of the test sample Dieda Guaishangling tincture (the raw material ratio of Dieda Guaishangling tincture: 17.7% of Potiancai, 10.6% of Chuifengsan, 15.9% of Schisandra chinensis, 5.3% of Guizhi, 13.3% of Liangmianzhen, 2.7% of Shengkuowu, 17.7% of Liaogewang, 3.5% of Jiulongchuan, and 13.3% of Dafengai), add 30 ml of 50% ethanol, ultrasonically treat at 310 W for 30 min, filter, and obtain the filtrate. Evaporate to dryness for 1 h, dissolve the residue in 20 mL of aqueous solution with a pH value of 4, filter, extract twice with petroleum ether (30-60°C), 20 ml / time, each extraction for 10 min, discard the petroleum ether layer, adjust the acid water layer to pH 8 with sodium hydroxide solution, filter, extract the filtrate with ethyl acetate twice, 20 ml / time, each extraction for 10 min, combine the ethyl acetate layers, evaporate to dryness for 20 min, dissolve the residue in 1 mL of methanol as the test solution.

[0040] (2) Preparation of medicinal material solution and control medicinal material solution: Take 0.5 g of Schisandra chinensis medicinal material and control medicinal material, add 20 ml of methanol respectively, treat with ultrasound at 310W for 30 min, filter, evaporate the filtrate to dryness, dissolve methanol to 1 ml, and prepare medicinal material solution and control medicinal material solution.

[0041] (3) Preparation of negative sample solution lacking Schisandra chinensis: Take 2.5 g of negative sample lacking Schisandra chinensis (17.7% of Potiancai; 10.6% of Chuifengsan; 5.3% of Guizhi; 13.3% of Liangmianzhen; 2.7% of Shengkuowu; 17.7% of Liaogewang; 3.5% of Jiulongchuan; 13.3% of Dafengai) and prepare the negative sample solution lacking Schisandra chinensis according to the sample preparation method.

[0042] Thin layer chromatography conditions: 10 μL of the test solution, medicinal material solution, control medicinal material solution, and Schisandrae Chinensis negative sample solution were respectively spotted on the same silica gel GF254 thin layer plate, and cyclohexane-ethyl acetate (3:2) was used as the developing solvent. The plate was developed, taken out, dried, and examined under ultraviolet light (254 nm).

[0043] This example takes 5.4 hours to complete. The thin layer spectrum is as follows Figure 2 As shown in the figure, the test sample chromatogram shows spots of the same color at the corresponding positions of the control medicinal material chromatogram. The spots are well separated, do not interfere with each other, have no tailing phenomenon, and have no negative interference.

[0044] Example 3

[0045] (1) Preparation of test solution: Take 3 g of fine powder of the test sample Dieda Guaishangling tincture (the raw material ratio of Dieda Guaishangling tincture: 17.7% of Potiancai, 10.6% of Chuifengsan, 15.9% of Schisandrae Chinensis, 5.3% of Cinnamomum cassia, 13.3% of Zanthoxylum bungeanum, 2.7% of Radix Aconiti Kusnezoffii, 17.7% of Rhizoma Leonuri, 3.5% of Rhizoma Jiulongchuan, and 13.3% of Artemisia selengensis), add 30 ml of 80% ethanol, ultrasonically treat at 300 W for 40 min, filter, and obtain the filtrate. Evaporate to dryness for 1 h, dissolve the residue in 20 mL of aqueous solution with a pH value of 1, filter, extract twice with petroleum ether (30-60°C), 20 ml / time, each extraction for 10 min, discard the petroleum ether layer, adjust the acid water layer to pH 7 with sodium hydroxide solution, filter, extract the filtrate with ethyl acetate twice, 20 ml / time, each extraction for 10 min, combine the ethyl acetate layers, evaporate to dryness for 30 min, dissolve the residue in 1 mL of methanol as the test solution.

[0046] (2) Preparation of medicinal material solution and control medicinal material solution: Take 0.5 g of Schisandra chinensis medicinal material and control medicinal material, add 20 ml of methanol respectively, treat with ultrasound at 320W for 20 min, filter, evaporate the filtrate to dryness, dissolve methanol to 1 ml, and prepare medicinal material solution and control medicinal material solution.

[0047] (3) Preparation of negative sample solution lacking Schisandra chinensis: Take 2.5 g of negative sample lacking Schisandra chinensis (17.7% of Potiancai; 10.6% of Chuifengsan; 5.3% of Guizhi; 13.3% of Liangmianzhen; 2.7% of Shengkuowu; 17.7% of Liaogewang; 3.5% of Jiulongchuan; 13.3% of Dafengai) and prepare the negative sample solution lacking Schisandra chinensis according to the sample preparation method.

[0048] Thin layer chromatography conditions: 10 μL of the test solution, medicinal material solution, control medicinal material solution, and Schisandrae Chinensis negative sample solution were respectively spotted on the same silica gel GF254 thin layer plate, and cyclohexane-ethyl acetate (3:2) was used as the developing solvent. The plate was developed, taken out, dried, and examined under ultraviolet light (254 nm).

[0049] This example takes 5.6 hours to complete. The thin layer spectrum is as follows Figure 3 As shown in the figure, the test sample chromatogram shows spots of the same color at the corresponding positions of the control medicinal material chromatogram. The spots are well separated, do not interfere with each other, have no tailing phenomenon, and have no negative interference.

[0050] Comparative Example 1

[0051] Take 1g of the same test sample powder as in Example 1, add 45mL of ethyl acetate, ultrasonicate at 320W for 30min, filter, concentrate the filtrate to dryness, add 1mL of ethyl acetate to dissolve it, and use it as the test solution. Take 1g of the same control medicinal material as in Example 1, and prepare a control medicinal material solution by the same test solution preparation method. Take the same Schisandrae Chinensis Negative Sample as in Example 1, and prepare a Schisandrae Chinensis Negative Sample Solution by the test solution preparation method. Take 6μL of the above test sample solution, medicinal material solution, and 4μL of the Schisandrae Chinensis Negative Sample Solution, and spot them on the same silica gel GF254 thin layer plate, use cyclohexane-ethyl acetate (3:1) as the developing agent, develop, take out, dry, and inspect under ultraviolet light (254nm).

[0052] This comparative example took about 3 hours to complete. The thin layer spectrum is as follows Figure 4 As shown in the figure, the spots are not clear, which means that they can only be used to identify medicinal materials, but cannot be used to identify traditional Chinese medicine preparations containing Schisandra chinensis.

[0053] Comparative Example 2

[0054] Take 30mL of the same test sample as in Example 1, evaporate to dryness, add 50ml of methanol, ultrasonic (power 90W, frequency 40kHz) for 25 minutes, place for 12 hours, take 20ml of the supernatant, add 3 times the amount of ether and place for 1 hour, pour out the supernatant, add 50ml of 10% hydrochloric acid solution to the precipitate, reflux in a boiling water bath for 1 hour, filter, dissolve the precipitate with 10ml of methanol, and concentrate the methanol solution to 1ml as the test solution. Take 2g of the same Schisandrae chinensis control medicinal material as in Example 1, and prepare the control medicinal material solution according to the test solution preparation method. Prepare the Schisandrae chinensis negative sample according to the process preparation method, take 20 pieces of the same negative sample as in Example 1, and prepare the negative control solution according to the test solution preparation method. According to the thin layer chromatography test, 10 μl of the test solution, negative control solution, and 5 μl of the control medicinal material solution were taken and spotted on the same silica gel G thin layer plate, and developed with n-hexane-chloroform-acetone-acetic acid (0.2:8:4:0.5) as the developing solvent, taken out, dried, sprayed with 8% vanillin anhydrous ethanol-sulfuric acid solution (7-10), and heated at 105°C until the spots were clearly colored. Place under sunlight for inspection.

[0055] This comparative example took about 17 hours to complete. The thin layer spectrum is as follows Figure 5 As shown, the spots are unclear, have tails, and cannot be reproduced.

[0056] In summary, the present invention improves the processing method of the sample to be tested, and forms a new thin layer identification method together with a thin layer plate, a developing agent (cyclohexane-ethyl acetate), and 254nm ultraviolet light. It can not only identify the single medicinal material of Schisandra chinensis, but also can better identify the compound preparation of Schisandra chinensis Chinese patent medicine. The components have good separation and no interference, and can effectively shorten the identification time and reduce the production inspection cost.

[0057] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof, characterized in that: The steps include: (1) Preparation of test solution: Take a Chinese medicine preparation containing Schisandrae Chinensis as a test sample, add ethanol solution, ultrasonically treat, filter, evaporate the filtrate to dryness, dissolve the residue in acidic aqueous solution, extract with petroleum ether, discard the petroleum ether layer, adjust the pH of the acidic aqueous layer, filter, extract the filtrate with ethyl acetate, combine the ethyl acetate layers, evaporate to dryness, and dissolve the residue in methanol to obtain a test solution; (2) Preparation of medicinal material solution: Take Schisandra chinensis medicinal material to prepare medicinal material solution; (3) Preparation of control medicinal material solution: Take Schisandra chinensis control medicinal material and prepare it according to the medicinal material solution preparation method; (4) Preparation of negative sample solution of Schisandrae chinensis deficiency: Take Schisandrae chinensis deficiency preparation as the sample to be tested and prepare it according to the method of the test solution; (5) The test sample solution, medicinal material solution, control medicinal material solution, and Schisandrae Chinensis negative sample solution are respectively spotted on a thin layer plate, developed with a developing agent, dried, developed, and inspected.

2. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the Chinese medicinal preparation containing Schisandra schizonepetae is Dieda Gushangling tincture.

3. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the mass concentration of the ethanol solution is 50%-80%.

4. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the ultrasonic power is 300-320W, and the ultrasonic time is 20-40min.

5. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the pH of the acidic aqueous solution is 1-4.

6. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the boiling range of the petroleum ether is 30-60°C.

7. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the pH of the acid water layer is adjusted to 7-8.

8. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (1), the filtrate is evaporated to dryness for 1-2 hours; the petroleum ether extraction is for 20-40 minutes; the ethyl acetate extraction is for 20-40 minutes; and the ethyl acetate layers are combined and evaporated to dryness for 20-40 minutes.

9. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (2), the method for preparing the medicinal material solution is: taking Schisandra chinensis medicinal materials, adding methanol, ultrasonically treating, filtering, evaporating the filtrate to dryness, and dissolving it with methanol to obtain the medicinal material solution.

10. The method for simultaneously identifying Schisandrae Chinensis medicinal materials and preparations thereof according to claim 1, characterized in that: In step (5), the developing agent is cyclohexane-ethyl acetate (3-5:2), and the inspection condition is 254nm ultraviolet light.

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