Detection box

Abstract

The invention provides a detection box which comprises a containing cavity, a bottom plate is arranged in the containing cavity, a sample inlet is formed in the bottom plate, a fence is arranged on the periphery of the containing cavity, a hole is formed in the lower portion of the fence or the joint of the bottom plate and the fence, the hole is communicated with the interior of the detection box, and when a sampling pad of a sample collector is located in the containing cavity, the sampling pad of the sample collector is located in the containing cavity. And a gap is formed between the hole and the sampling pad. By adopting the detection box, the liquid sample can be effectively prevented from overflowing or splashing out of the sample accommodating cavity in the process of extruding the liquid sample into the detection box from the sample collector, and the detection box is compact and small in structure, is very convenient to carry, belongs to a portable detection device, and is suitable for popularization and application. The kit can be used for detection such as disease diagnosis, drunk driving test, drug suction condition analysis, hormone condition analysis and the like, and the field detection requirements of rapid sampling and rapid detection are met.

Description

[0001] This application is a divisional application of a patent application with an application date of September 7, 2022, an application number of 202211091536X, and an invention name of detection box. Technical Field

[0002] The present invention relates to the technical field of rapid medical detection, and in particular to a detection box and a detection device for medical detection. Background Art

[0003] The carrier of traditional disposable rapid detection products is basically in the form of test strips or test boxes. For example, the test strips of detection products using lateral flow generally include a bottom card, on which a sample pad, a marker binding pad (also referred to as a marker pad, usually using glass fiber as a carrier), a test pad (usually using NC membrane as a carrier) and a water-absorbing pad (usually using a water-absorbing material such as filter paper) are adhered one after another from upstream to downstream, and the immunochromatography principle is used to realize the transfer of samples on the test strip and obtain the test results. The marker pad includes a marker that can bind to the analyte, such as latex, colloidal gold, fluorescent microspheres, etc. labeled with antigens or antibodies. The test pad is generally provided with a test line and a quality control line. As the sample flows on the test strip, the marker will be captured and aggregated or not captured on the test line. According to the signal of the marker, such as a color signal or a fluorescent signal, it is judged whether the analyte exists or its concentration. The quality control line can be used to judge whether the test strip is effective or for positioning when the instrument reads the test results. The test box is a test paper placed between an upper cover and a lower plate. A sample addition hole is opened on the upper cover corresponding to the test paper sample pad, and an observation window is opened on the upper cover corresponding to the test pad.

[0004] Disposable rapid testing and diagnostic products that use body fluids such as urine, blood or other tissue fluids to detect diseases or other physical conditions have been widely used throughout the world. They can be used in laboratories operated by professionals or by non-professionals at home, schools, shopping malls, road checkpoints, customs and other places.

[0005] This type of traditional testing product is just a simple test strip or test kit. When using a sampling stick for sampling and testing, it is necessary to put the sampling stick into a collection bottle after sampling, squeeze the sample on the sampling stick into the collection bottle, and then drip the sample into the sample pad or the sample addition hole, such as US patent application US20040237674A1. Figure 7 and Figure 8As shown, such a sample addition operation is rather cumbersome. Chinese patent CN200420110153.3 and Chinese patent CN201010164579.7 have improved the test box respectively and simplified the sample addition operation steps. The sample addition hole of the test box of Chinese patent CN200420110153.3 protrudes from the upper cover of the test box to form a receiving cavity, and the internal space of the receiving cavity can accommodate the water-absorbing material on the sampling rod and place it therein, and squeeze the liquid sample onto the sample pad by squeezing. The sample addition port of Chinese patent CN201010164579.7 is provided with a channel, which allows the sampling head to pass through, squeezes out the liquid sample by squeezing, and transfers the sample to the sample pad through the drainage piece. The sampling holes of these two Chinese patents have the following problems, for example: the user needs to keep pressing the sampling stick to maintain the squeezing state until the liquid sample is completely squeezed out. If the sampling stick is released halfway, the liquid sample remaining on the sampling stick will not be able to reach the sample pad after the sampling stick loses the squeezing force, increasing the risk of insufficient sampling; the sampling stick may also fall out of the sampling hole or the sampling channel after being released and pollute the surrounding environment; the sampling hole or the sampling channel arranged on the upper cover is blocked by the sampling stick during sampling, and the liquid sample cannot be transferred to the test paper below in time and is transported away, but accumulates in the sampling area. The squeezed sample may be squeezed by the sampling stick and overflow or splash out of the sampling area, or even splash onto the operator's body, causing danger to the operator.

[0006] Therefore, how to provide a detection device to achieve rapid detection and prevent the sample from overflowing from the sample addition area during the sample addition process is a technical problem that technicians in this field urgently need to solve. Summary of the invention

[0007] One of the purposes of the present invention is to provide a detection box to solve the problems existing in the prior art. The detection box includes a storage chamber, a bottom plate is provided in the storage chamber, a sample inlet is provided on the bottom plate, a fence is provided on the periphery of the storage chamber, a hole is provided at the lower part of the fence or at the connection between the bottom plate and the fence, and the hole is connected to the interior of the detection box.

[0008] Furthermore, the detection box includes an observation window for observing the detection results, the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure, or the hole is arranged at the lower part of at least one side enclosure.

[0009] Furthermore, the detection box includes an observation window for observing the detection results, and the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure and the lower part of at least one side enclosure.

[0010] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure, a leakage hole arranged at the lower part of the rear enclosure, and a side leakage hole arranged at the lower part of at least one side enclosure, or the hole includes at least one of the guide groove, the leakage hole and the side leakage hole.

[0011] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure, and the guide groove and the drainage hole are separated by an isolation block.

[0012] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure, and the guide groove is connected to the drainage hole to form an "L"-shaped opening.

[0013] Furthermore, the storage cavity includes a first buckle.

[0014] Furthermore, the first buckle is arranged on the bottom plate between the injection port and the wall of the receiving chamber, and a certain spacing space is left between the first buckle and the wall of the receiving chamber.

[0015] Furthermore, the first buckle and the hole are respectively located on two opposite sides of the injection port on the bottom plate.

[0016] Furthermore, it includes a test paper located inside the test box.

[0017] Furthermore, when the sampling pad of the sample collector is located in the receiving cavity, there is a gap between the hole and the sampling pad.

[0018] Furthermore, the detection box includes an upper cover and a lower plate, and the storage cavity is located on the upper cover.

[0019] Furthermore, a storage slot for placing the test paper is provided on the lower plate, the test paper includes a sample pad, and the lower plate also includes a liquid storage tank, which is located at the sample pad end of the storage slot.

[0020] Furthermore, a notch is provided on the storage tank in the liquid storage tank.

[0021] Furthermore, the notch is adjacent to the sample pad of the test paper.

[0022] Furthermore, the notch divides the wall of the storage tank in the liquid storage tank into two parts.

[0023] Furthermore, the guide groove, the leakage hole and the side leakage hole are all located above the liquid storage tank, and the orthographic projections of the guide groove, the leakage hole and the side leakage hole are located in the liquid storage tank; the guide groove, the leakage hole and the side leakage hole are respectively connected to the liquid of the liquid storage tank.

[0024] One of the purposes of the present invention is to provide a detection device to solve the problem of quickly introducing the added liquid sample into the detection box during the detection process.

[0025] In order to achieve this goal, the present invention provides the following technical solutions.

[0026] The present invention provides a detection device, including a detection box and a sample collector. The detection box includes a storage cavity, a bottom plate is provided in the storage cavity, a sample inlet is provided on the bottom plate, an enclosure is provided on the periphery of the storage cavity, a hole is provided at the lower part of the enclosure or at the connection between the bottom plate and the enclosure, the hole is connected to the interior of the detection box, and when a sampling pad of the sample collector is located in the storage cavity, there is a gap between the hole and the sampling pad.

[0027] A hole is provided in the storage cavity for timely guiding out the liquid sample, and an appropriate gap is provided between the hole and the sampling pad of the sample collector, so that the hole will not be blocked by the sampling pad, and the liquid flow channel is unobstructed, so that the liquid sample squeezed out from the sampling pad can be guided away quickly and timely, thereby more effectively preventing the liquid sample from overflowing or splashing out of the sample storage cavity during the process of squeezing the liquid sample from the sample collector into the detection box, thereby ensuring that the environment around the detection will not be polluted due to the overflow of the liquid sample during the detection process, and the liquid sample will not splash out and fall on the operator's body, thereby reducing the risk of physical harm to the operator.

[0028] Furthermore, the detection box includes an observation window for observing the detection results, the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure, or the hole is arranged at the lower part of at least one side enclosure.

[0029] Furthermore, the detection box includes an observation window for observing the detection results, and the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure and the lower part of at least one side enclosure.

[0030] Furthermore, the hole provided at the lower portion of the side enclosure is close to the rear enclosure.

[0031] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure, a leakage hole arranged at the lower part of the rear enclosure, and a side leakage hole arranged at the lower part of at least one side enclosure, or the hole includes at least one of the guide groove, the leakage hole and the side leakage hole.

[0032] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure, and the guide groove and the drainage hole are separated by an isolation block.

[0033] Furthermore, the hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure, and the guide groove is connected to the drainage hole to form an "L"-shaped opening.

[0034] Furthermore, the storage cavity includes a first buckle.

[0035] Furthermore, the first buckle is arranged on the bottom plate between the injection port and the wall of the receiving chamber, and a certain spacing space is left between the first buckle and the wall of the receiving chamber.

[0036] Furthermore, the first buckle includes a protrusion and a clamping arm, and a recess is provided at the front end of the sample collector. When the sample collector is inserted into the storage cavity of the detection box, the protrusion of the first buckle is buckled into the recess of the collector.

[0037] Furthermore, the first buckle and the hole are respectively located on two opposite sides of the injection port on the bottom plate.

[0038] Beneficial Effects

[0039] The detection box of the present invention is provided with a hole in the storage cavity that can timely guide the liquid sample, so that the liquid collected in the storage cavity can flow into the interior of the detection box through the hole, thereby preventing all the liquid from flowing into the detection box from the sampling port, causing the detection test paper in the detection box to generate a flood phenomenon and ultimately lead to detection failure; and the liquid can be quickly circulated to the interior of the detection box through the hole, thereby preventing the liquid sample from overflowing or splashing out of the sample storage cavity during the process of squeezing the liquid sample from the sample collector into the detection box, thereby ensuring that the environment around the detection will not be polluted due to the overflow of the liquid sample during the detection process, and the liquid sample will not splash out and fall on the operator's body, thereby reducing the risk of physical harm to the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a schematic diagram of the detection device of the present invention in use, that is, a schematic diagram when the sample collector is just inserted into the detection box.

[0041] Figure 2 This is a schematic diagram of the detection device of the present invention in use, showing a schematic diagram when the sample collector is fully inserted into the detection box.

[0042] Figure 3 It is a three-dimensional exploded schematic diagram of the detection device of the present invention.

[0043] Figure 4 Schematic diagram of a lateral flow test strip.

[0044] Figure 5 is a schematic diagram of the sample collector.

[0045] Figure 6 It is a schematic diagram of the first embodiment of the detection box of the present invention, wherein a guide groove is provided on the bottom plate of the detection box storage cavity, a drainage hole is provided at the lower part of the rear enclosure, and a side drainage hole is provided at the lower part of the side enclosure.

[0046] Figure 7 yes Figure 6 Front view of the test cassette.

[0047] Figure 8 yes Figure 7 Cross-section view in the AA direction.

[0048] Fig. 9 yes Figure 7 Cross-section view in the BB direction.

[0049] Fig.10 It is a schematic diagram of a second embodiment of the detection box of the present invention. A guide groove is provided on the bottom plate of the storage chamber of the detection box, and no leakage hole is provided at the lower part of the fence.

[0050] Fig.11 It is a top view of the sample collector inserted into the test box.

[0051] Fig.12 It is a three-dimensional schematic diagram of the lower plate of the test box (with test strips installed).

[0052] Fig.13 It is a top view of the lower plate of the test box (without test strips installed).

[0053] Fig.14 It is a plan view of the inner surface of the upper cover and the inner surface of the lower plate of the detection box, and the double-headed arrows indicate that the upper cover and the lower plate can be buckled together.

[0054] Fig.15 yes Fig.10 The partial enlarged schematic diagram of the detection box at "N" shown corresponds to the second embodiment of the detection box of the present invention. A guide groove is opened on the bottom plate of the storage cavity of the detection box, but no drainage holes are opened at the lower part of the rear enclosure and the lower part of the side enclosure.

[0055] Fig.16 yes Figure 6 The partial enlarged schematic diagram of the detection box at M shown corresponds to the first embodiment of the detection box of the present invention. A guide groove is opened at the bottom of the storage cavity of the detection box, a drainage hole is opened at the lower part of the rear enclosure, and a lateral drainage hole is opened at the lower part of the side enclosure. The guide groove, the drainage hole and the lateral drainage hole are completely connected, and there is no isolation block between them.

[0056] Fig.17 and Fig.16 Similarly, corresponding to the third embodiment of the detection box of the present invention, a guide groove is opened at the bottom of the detection box storage cavity, and a drainage hole is opened at the lower part of the rear enclosure. The guide groove and the drainage hole are interconnected without any isolation blocks, but no lateral drainage holes are set at the lower part of the side enclosure.

[0057] Fig.18 and Fig.16 Similarly, corresponding to the fourth embodiment of the detection box of the present invention, a guide groove is opened at the bottom of the detection box storage cavity, a drainage hole is opened at the lower part of the rear enclosure, and an isolation block is arranged between the guide groove and the drainage hole, but no lateral drainage hole is arranged at the lower part of the side enclosure.

[0058] Fig.19 and Fig.16 Similarly, corresponding to the fifth embodiment of the detection box of the present invention, a leakage hole is opened at the lower part of the rear enclosure of the detection box, and a lateral leakage hole is opened at the lower part of the side enclosure.

[0059] Fig. 20 and Fig.16 Similarly, corresponding to the sixth embodiment of the detection box of the present invention, a guide groove is opened at the bottom of the storage cavity of the detection box, and a lateral leakage hole is opened at the lower part of the side enclosure.

[0060] Fig.21 and Fig.16 Similarly, corresponding to the seventh embodiment of the detection box of the present invention, a lateral leakage hole is opened at the lower part of the side enclosure of the detection box.

[0061] Fig. 22 and Fig.16 Similarly, corresponding to the eighth embodiment of the detection box of the present invention, a leakage hole is provided at the lower portion of the rear enclosure of the detection box.

[0062] Fig.23 yes Fig.11 The cross-sectional schematic diagram along the CC direction shows the state where the sample collector has just been inserted into the detection box.

[0063] Fig.24 and Fig.23 Similar, but shows the sample collector in the process of being pressed down onto the test cartridge.

[0064] Fig.25 and Fig.23 Similar, but it shows the state where the sample collector is fully pressed into the receiving cavity.

[0065] Fig.26 yes Fig.25 A zoomed in view at P, which corresponds to Fig.15 The embodiment shown.

[0066] Fig. 27 and Fig.26 Similarly, it corresponds to Fig.16 or Fig.17 The embodiment shown.

[0067] Fig.28 and Fig.26 Similarly, it corresponds to Fig.18 The embodiment shown. DETAILED DESCRIPTION

[0068] The present invention is described in detail below in conjunction with specific drawings. These specific embodiments are only limited enumerations without violating the spirit of the present invention, and do not exclude other specific implementation schemes produced by ordinary technicians in the field by combining the prior art with the present invention.

[0069] Definition: The "lower part" in this patent specification includes the "bottom" of a component and / or the part extending upward from the "bottom" of the component.

[0070] like Figures 1 to 9 The detection device shown includes a detection box 300 and a sample collector 200 used in conjunction with the detection box. The detection box includes an upper cover 1, a lower plate 2 and a detection test paper 100, and the detection test paper 100 is located between the upper cover and the lower plate of the detection box. In one embodiment, the upper cover 1 and the lower plate 2 are buckled together to install the detection test paper 100 in the detection box.

[0071] like Figure 4 The test strip 100 shown is a lateral flow test strip, which includes a bottom card 101, on which a sample pad 102, a marker binding pad 103, a test pad 104 and a water absorbent pad 105 are adhered one on top of the other in sequence from upstream to downstream. The test strip can also be a vertical flow test strip, or other forms of test strips.

[0072] like Figure 5 The collector 200 shown includes a handle 201, a connection portion 202 and a sampling head 203. The sampling head 203 includes a sampling pad 204. The sampling pad 204 can be made of absorbent cotton strips, sponges, absorbent fibers or porous polymer materials, or absorbent materials that change from hard to soft after absorbing water, such as polyvinyl alcohol. The mounting plate 203 can also be provided with an indication area 205, which is located above the sampling pad, and an indication test paper is stored in the indication area. When the sampling pad absorbs enough liquid sample, the liquid sample will contact the indication test paper, so that the indication test paper changes from the first color to the second color. The operator can judge whether the sample is collected enough according to the color change of the indication test paper. The handle 201 can be designed to be a flat configuration with a certain curvature for the operator to grasp, and the connection portion 202 is designed to be a thin neck shape.

[0073] like Figure 6 The upper cover 1 of the test box 300 shown is provided with a sample inlet 3 and an observation window 4. The sample inlet 3 is located above the sample pad 102 of the test paper, and the observation window 4 is located above the test pad 104 of the test paper. A fence 5 is provided in the surrounding area of ​​the sample inlet 3. The fence 5 can be erected on the upper surface of the upper cover to enclose the sample inlet 3 therein and form a storage cavity 6; the fence 5 can also be sunken from the upper surface of the upper cover and extend into the test box to enclose the sample inlet 3 therein and form a storage cavity 6. The fence 5 can also be partially erected on the upper surface of the upper cover, and partially sunk into the inside of the test box to enclose the sample inlet 3 therein and form a storage cavity 6.

[0074] A bottom plate 7 is provided in the receiving chamber 6, and the bottom plate can cooperate with the sampling pad to squeeze the liquid out of the sampling pad by the cooperation between the bottom plate and the sampling pad. Therefore, the bottom plate can also be called an extrusion plate. The sampling port 3 is provided on the bottom plate. Figure 6 As shown, a portion of the upper cover plate enclosed in the storage cavity 6 can be used as an extrusion plate 7 that cooperates with the sampling pad, and the sample inlet 3 is located on the extrusion plate 7. A plurality of sample inlets 3 can be arranged above the sample pad 102 of the test paper, for example, two sample inlets are arranged at intervals, which can, on the one hand, quickly introduce the extruded sample into the sample pad 102 of the test paper, and on the other hand, maintain the strength of the extrusion plate 7 so that it is not easily deformed during the extrusion process.

[0075] In the present invention, a hole is provided at the lower part of the enclosure 5 or at the connection between the bottom plate 7 and the enclosure 5, and the hole is connected to the inside of the detection box 300, and when the sampling pad of the sample collector 200 is located in the storage chamber 6, there is a gap 21 (such as Figure 26-Figure 28 The enclosure 5 includes a front enclosure 51, a rear enclosure 52 and two side enclosures 53 (as shown in FIG. Figure 6 When the sampling pad 204 of the sample collector 200 is inserted into the storage chamber 6, the front enclosure 51 is located in the top direction of the sample collector, and the rear enclosure 52 is located in the connection direction of the sample collector (as shown). Figure 1 and Figure 2 As shown). The front enclosure 51 is closer to the observation window 4 than the rear enclosure 52. The hole is provided at the lower part of the rear enclosure 51 or at the lower part of at least one side enclosure. Alternatively, the hole is provided at the lower part of the rear enclosure and at least one side enclosure. Preferably, the hole provided at the lower part of the side enclosure 53 is close to the rear enclosure 52. More specifically, the hole includes a guide groove 8 provided at the connection between the bottom plate 7 and the rear enclosure 52, a drainage hole 16 provided at the lower part of the rear enclosure 52, and a side drainage hole 24 provided at the lower part of at least one side enclosure 53 (as shown). Fig.16or the hole includes at least one of the guide groove 8, the leakage hole 16 and the side leakage hole 24, that is, the hole can be one of the guide groove 8, the leakage hole 16 and the side leakage hole 24 as described above, or can be a combination of any two of the guide groove 8, the leakage hole 16 and the side leakage hole 24 as described above, or can also include the guide groove 8, the leakage hole 16 and the side leakage hole 24 as described above (such as Figure 15-Figure 22 Alternatively, the hole includes a guide groove 8 provided at the connection between the bottom plate and the rear enclosure and a drain hole 16 provided at the lower part of the rear enclosure, wherein the guide groove 8 and the drain hole 16 are separated by an isolation block 54 (as shown in FIG. Fig.18 Alternatively, the hole includes a guide groove 8 provided at the connection between the bottom plate 7 and the rear enclosure 52 and a drain hole 16 provided at the lower part of the rear enclosure 52, wherein the guide groove 8 is connected with the drain hole 16 to form an "L"-shaped opening (as shown in FIG. Fig.17 shown).

[0076] The area in the storage chamber 6 near the front enclosure 51 can be called the front end of the storage chamber 6, and the area in the storage chamber 6 near the rear enclosure 52 can be called the rear end of the storage chamber 6. After the sampling pad 204 of the sample collector is located in the storage chamber 6, there is an appropriate gap 21 between the hole located at the lower part of the enclosure 5 or the connection between the bottom plate 7 and the enclosure 5 and the sampling pad, and the gap 21 is connected to the hole, so that the hole will not be blocked by the sampling pad 204, and the hole is connected to the interior of the detection box. Therefore, the present invention provides a channel for the liquid sample squeezed into the storage chamber 6 to quickly flow into the interior of the detection box, thereby solving the technical problem in the prior art that the liquid sample splashes due to failure to be discharged in time. Although Fig.26 , Fig. 27 and Fig.28 It is only shown that the gap 21 is located between the guide groove 8 and / or the leakage hole 16 and the sampling pad 204, but the gap 21 can also be located between the side leakage hole 24 and the sampling pad 204 (not shown). That is to say, the purpose of setting the gap 21 in the present invention is to avoid the guide groove 8, the leakage hole 16 or the side leakage hole 24 (if the guide groove 8, the leakage hole 16 or the side leakage hole 24 exist) from being blocked by the sampling pad 204. Therefore, a preferred embodiment of the present invention is that if the guide groove 8, the leakage hole 16 or the side leakage hole 24 exist, then the gap 21 should be set between the guide groove 8, the leakage hole 16 or the side leakage hole 24 and the sampling pad 204. The size of the gap 21 is preferably such that the liquid sample can flow quickly into the interior of the detection box, thereby avoiding the splashing of the liquid sample, and does not affect the portability and ease of operation of the detection device.

[0077] Figure 6The test box shown in the figure is provided with three guide grooves 8 at the connection between the extrusion plate 7 and the rear enclosure 52. The guide grooves 8 penetrate the extrusion plate 7. Therefore, the guide grooves 8 are connected with the inside of the test box, so that the squeezed liquid sample can be introduced into the liquid storage tank 18 in the test box or the sample pad 102 of the test paper, and the sample in the liquid storage tank can be further absorbed by the sample pad of the test paper.

[0078] like Figures 6 to 9 As shown, a drainage hole 16 is provided at the lower part of the rear enclosure 52 of the storage chamber 6, and the drainage hole 16 penetrates the extrusion plate 7. The drainage hole 16 is connected to the inside of the detection box, so that the liquid sample flowing to the rear enclosure can enter the liquid storage tank 18 of the detection box through the drainage hole 16. The guide groove 8 and the drainage hole 16 are both located above the liquid storage tank 18. Specifically, the orthographic projections of the guide groove 8 and the drainage hole 16 are both located in the liquid storage tank. In addition, the guide groove 8 and the drainage hole 16 are respectively connected to the liquid of the liquid storage tank 18.

[0079] like Figure 6 and Figure 7 In the example shown, the guide groove 8 on the extrusion plate corresponds to the leakage hole 16 on the lower edge of the rear enclosure 52, and the guide groove 8 extends all the way to the leakage hole 16 at the lower part of the rear enclosure. More specifically, the guide groove 8 on the extrusion plate and the leakage hole 16 on the rear enclosure are connected to form a right-angle opening or an L-shaped opening. Such a design can further increase the flux of liquid samples flowing into the liquid reservoir of the detection box, and avoid too many samples being retained on the extrusion plate of the storage chamber during the process of squeezing the sample collector, causing the liquid sample to splash out of the storage chamber. Among them, the lateral leakage hole 24 is also located above the liquid reservoir 18. Specifically, the positive projection of the lateral leakage hole 24 is located in the liquid reservoir, and the two are liquid-connected.

[0080] Figure 6 A side drain hole 24 is also provided at the lower part of the side enclosure at the rear end of the storage chamber of the detection box to further increase the drainage capacity of the rear end of the storage chamber.

[0081] like Figure 6 and Fig.16 The structure of the rear end of the detection box storage chamber shown in the figure has a guide groove 8 on the extrusion plate, a drainage hole 16 on the lower part of the rear enclosure, and a lateral drainage hole 24 on the lower part of the side enclosure. The guide groove extends to the drainage hole 16 and the lateral drainage hole 24 respectively, and intersects with the drainage hole 16 and the lateral drainage hole 24. The drainage hole 16 and the lateral drainage hole 24 are connected. Therefore, at the two corners of the rear part of the storage chamber, an opening composed of the guide groove 8, the drainage hole 16 and the lateral drainage hole 24 is formed to drain the liquid sample gathered at the rear part of the storage chamber, as shown in FIG. Fig. 27 shown.

[0082] Fig.10 and Fig.15It is another design of the detection box of the present invention. Fig.10 and Fig.15 The bottom plate of the test box shown in the figure is provided with a guide groove 8 , the lower part of the rear enclosure 52 of the test box is not provided with a drainage hole 16 , and the lower part of the side enclosure 53 is not provided with a lateral drainage hole 24 .

[0083] Fig.23 , Fig.24 , Fig.25 and Fig.26 Shows the use of Fig.10 and Fig.15 The operation process of the detection box is shown.

[0084] Fig.17 The rear end of the receiving chamber of the test box is provided with a guide groove 8 and a drain hole 16, and the guide groove 8 extends to the bottom of the drain hole 16. Fig.17 In the detection box of the structure shown in FIG. Fig. 27 As shown, when the sample collector is fully pressed into the storage cavity, although the guide groove 8 is mostly covered by the sampling pad 204, the liquid flowing to the rear end of the storage cavity can still flow into the liquid storage tank in the detection box from the leakage hole 16 at the lower part of the rear enclosure. Due to the drainage effect of the leakage hole, when the sampling pad is completely in contact with the extrusion plate, it is not enough to make the liquid remaining in the storage cavity flow out along the assembly gap between the sample collector and the detection box or splash out of the storage cavity.

[0085] Fig.18 The rear end of the receiving chamber of the test box shown in the figure is provided with a guide groove 8 and a drain hole 16, and an isolation block 54 is provided between the guide groove 8 and the drain hole 16. The function of the isolation block 54 is to improve the strength of the extrusion plate 7. Fig.28 As shown, insert the sample collector 200 into the Fig.18 In the detection box of the structure shown, when the sample collector is completely pressed into the receiving chamber, due to the drainage effect of the leakage hole, only a very small amount of liquid is collected at the rear end of the receiving chamber. When the sampling pad is completely in contact with the squeezing plate, the liquid sample 500 ( Fig.28 In the figure, several "dots" are used to represent the liquid sample 500, the same as in the whole text). The amount of liquid is not enough to flow out or splash out of the storage cavity along the assembly gap between the sample collector and the detection box. Even if there is still some liquid at the rear end of the storage cavity and the liquid level is raised after being squeezed, the liquid level in the assembly gap can still flow out from the leakage hole into the detection box after being raised.

[0086] use Fig.17 A set of comparative experiments were conducted on the detection box shown in the figure and the detection box shown in the prior art (i.e., the detection device without a hole). The sample collector was inserted into Fig.17In the test box storage cavity shown and the existing test box storage cavity, observe the liquid sample splashing out from the rear enclosure. When using the existing test box, the squeezed liquid sample will splash out of the storage cavity, and the splashed liquid sample 500 will pollute the surrounding environment. Fig.17 When the test box is shown, the squeezed liquid sample will not splash out of the storage chamber. Experimental comparison shows that providing the leakage hole 16 in the storage chamber can effectively prevent the squeezed liquid sample from splashing out of the enclosure and polluting the surrounding environment.

[0087] The opening size and height of the leakage hole, the distance between the leakage hole and the extrusion plate (for example, the leakage hole can pass through the extrusion plate, or can be above the extrusion plate without passing through the extrusion plate), and the number of leakage holes can all be adjusted according to factors such as the sampling volume of the sample collector, the capacity of the storage chamber, and the injection speed of the injection port.

[0088] The experimental results show that the detection box used in the experimental group 1 is a detection box for the three methods of diversion groove, leakage hole and side leakage hole (such as Fig.16 The test box shown in the figure), the test box used in the experimental group 2 only uses the test box with two structures of the guide groove, the leakage hole or the side leakage hole (such as Fig.17 , 18, 19, 20), the test box used in experimental group 3 is a test box that only includes one of the guide grooves, the leakage holes and the side leakage holes (such as Fig.15 , 22, 21 test boxes), experimental group 1 is better at preventing liquid splashing than experimental group 2, and experimental group 2 is better at preventing liquid splashing than experimental group 3. The experiment also verified that the anti-splashing effect of opening a leakage hole on the rear fence of the storage cavity is better than the anti-splashing effect of only opening a guide groove on the bottom plate. The anti-splashing effect of opening a leakage hole on the rear enclosure is better than the lateral anti-splashing effect of opening a side fence.

[0089] like Figure 7 , Figure 8 and Fig. 9 As shown, a fastening device for the sample collector is also provided on the upper cover 1. One of the fastening devices is a first buckle 9 provided in the receiving cavity 6. Figure 1 and Figure 2 The sample collector 200 placed in the storage cavity 6 uses the first buckle 9 as a fulcrum to press the sample collector downward, so that the sampling pad 204 and the squeezing plate 7 are squeezed to squeeze out the liquid in the sampling pad. Figure 1 and Figure 2 ,as well as Figures 7 to 9 In the example shown, the upper end of the first buckle 9 is provided with a protrusion 91 (such as Figure 8 As shown), a recess 206 is provided at the top of the sample collector (as shown Figure 1As shown in the figure, when the sample collector is inserted into the storage cavity, the top of the sampling pad mounting plate 203 is placed below the first buckle 9, and the protrusion 91 of the first buckle 9 is buckled into the recess 206 of the sample collector. Then, the sample collector handle 201 is pressed down with the buckle as the axis to rotate the fulcrum, so that the sampling pad 204 and the squeezing plate 7 are squeezed. The fulcrum formed between the first buckle and the sample collector can also be formed in other forms, such as but not limited to the following methods, for example, the upper end of the first buckle 9 is set as a recess, and the top of the sample collector is set as a protrusion. Alternatively, an opening is set on the first buckle, and an insertion protrusion is set at the top of the sample collector, and the protrusion can be inserted into the opening to form a downward pressing fulcrum.

[0090] The first buckle 9 is disposed at the front end of the storage cavity. For example, the first buckle 9 can be disposed on the inner wall of the front enclosure 51. Figure 7 In the embodiment shown in FIG. 1 , the first buckle 9 is arranged on the bottom plate between the injection port 3 and the front enclosure 51, and a certain spacing space 11 (such as Figure 7 As shown). That is, the first buckle 9 and the hole (e.g., the guide groove 8) are located on opposite sides of the sample inlet 3 on the bottom plate. In the example where the first buckle is a buckle with a certain elasticity, the spacing space 11 leaves a retreat space for the first buckle 9. Specifically, when the sample collector is inserted into the storage chamber 6, the front end of the collector presses against the arm 92 of the first buckle 9 (e.g., Figure 8 As shown in the figure, after the clamp arm is compressed, it elastically tilts toward the spacing space until the protrusion 91 of the first clamp is engaged in the concave portion 206 of the collector, and the clamp arm returns to its original position.

[0091] In the embodiment where a spacing space 11 is provided between the first buckle 9 and the front enclosure 51, the spacing space 11 can also play a buffering role. In the manner where the first buckle 9 is provided on the inner wall of the front enclosure, the top of the sample collector 200 inserted into the storage cavity 6 is very close to the front enclosure. If the amount of liquid sample squeezed out from the front end of the sampling pad is greater than the assembly gap between the top of the collector and the front enclosure, and this part of the liquid does not have time to flow into the detection box, the liquid sample in the assembly gap between the top of the collector and the front enclosure may be squeezed out of the enclosure. Therefore, the spacing space 11 provided increases the temporary storage space of the liquid sample, which is equivalent to a buffer zone.

[0092] The present invention may also include another fastening device, which is a second buckle 10 disposed behind the rear enclosure 52. The slot 13 of the second buckle 10 is coaxial with the rear enclosure groove 12. When the sample collector 200 completely presses the sampling pad onto the extrusion plate 7 (bottom plate), the connecting portion 202 of the sample collector is inserted into the second buckle and confined in the slot 13 of the second buckle. After the operator releases the collector, the sampling pad remains in the extruded state.

[0093] like Figure 1 , Figure 2 and Figure 6 As shown, a groove 12 for receiving the connection part 202 of the sample collector is also opened on the rear enclosure 52. When the sample collector 200 is pressed down with the first buckle 9 as a fulcrum, the connection part 202 of the collector can be embedded in the groove 12 of the rear enclosure, so that the sampling pad 204 of the collector 200 can be completely pressed flat on the extrusion plate 7, so that every position of the sampling pad 204 can be fully squeezed.

[0094] The lower plate 2 of the test box is provided with a storage slot 17 for the test paper 100, and a liquid storage tank 18 is provided at the sample pad end of the storage slot. Fig.12 and Fig.13 In the illustrated embodiment, the reservoir wall 19 of the liquid reservoir and the groove wall 170 of the storage groove at the sample pad end are combined to form the liquid reservoir.

[0095] One function of the liquid reservoir is to collect and temporarily store the liquid sample squeezed out from the sample collector. The sampling hole of the test box should not be too large. If the sampling hole is too large, a large amount of liquid sample will instantly reach the detection pad of the test paper, causing the test paper to flood, affecting the test result. The test box of the present invention allows a part of the liquid sample to flow to the detection pad of the test paper through the sampling hole 3, and the other part of the liquid flows into the liquid reservoir of the test box through the flow-aiding hole and / or the leakage hole. The liquid sample in the liquid reservoir can continue to be slowly absorbed by the detection pad of the test paper until the detection dosage requirement is reached.

[0096] A notch 20 (such as Fig.12 , 13 The embodiment shown in FIG. 1 is a method for draining the liquid sample in the liquid reservoir to the test paper. The liquid sample collected in the liquid reservoir can enter the storage tank 17 through the notch 20 and be absorbed by the sample pad in the storage tank. The notch can more effectively guide the liquid in the liquid reservoir to contact the sample pad of the test paper.

[0097] like Fig.14As shown, the width of the bottom plate at the receiving cavity is smaller than the width of the lower plate liquid reservoir. When the upper cover and the lower plate are buckled together, the wall of the liquid reservoir surrounds the bottom 61 of the receiving cavity, and the liquid flowing into the liquid reservoir can be effectively controlled in the liquid reservoir, preventing the sample that is not absorbed by the sample pad from flowing around in the detection box. Fig.14 As shown, the lateral width of the bottom edge of the lower plate reservoir wall is 13.6 cm (as shown in FIG. Fig.14 The distance between the bottom edge of the pool wall and the bottom edge of the lower plate of the detection box is 12cm (as indicated by the distance at "A" of the detection box). Fig.14 The horizontal width of the bottom plate of the upper cover storage cavity is 13cm (as indicated by the distance at "B" of Fig.14 The minimum distance between the bottom plate of the upper cover storage cavity and the bottom edge of the upper cover of the detection box is 12.5 cm (as indicated by the distance at "C" of the upper cover). Fig.14 When the upper cover and the lower plate are buckled together, the wall of the liquid storage tank surrounds the bottom plate of the storage chamber.

[0098] Example 1 Preparation of a portable novel coronavirus detection device

[0099] Take the preparation of a portable new coronavirus (SARS-CoV-2) detection device as an example.

[0100] The novel coronavirus (SARS-CoV-2) test strip 100 comprises a base card 101, on which a sample pad 102, a marker binding pad 103, a test pad 104 and a water-absorbing pad 105 are adhered and stacked one on the other from upstream to downstream. The marker binding pad 103 is coated with an anti-novel coronavirus (anti-SARS-CoV-2) antibody-latex marker, the detection line (T line) of the test pad is coated with anti-SARS-CoV-2 antibodies, and the quality control line (C line) is coated with goat anti-mouse IgG.

[0101] The prepared novel coronavirus (SARS-CoV-2) test paper is loaded into the test box 300, so that the sample inlet 3 of the upper cover 1 of the test box is located above the sample pad 102 of the test paper, and the observation window 4 is located above the test pad 104 of the test paper.

[0102] The sample collector 200 is packaged in a sealed bag to form a complete detection device with the prepared detection box. The sampling pad of the sample collector 200 absorbs water and swells after contacting saliva.

[0103] Example 2 Use of the detection device

[0104] Take the use of a portable novel coronavirus (SARS-CoV-2) detection device as an example.

[0105] Take out the sample collector 200 from the sealed bag, put the sample collector into the tester's mouth to absorb saliva to the specified sample volume. If there is an indicator test paper, it can be judged whether a sufficient amount has been collected according to the color change of the indicator test paper. If there is no indicator test paper, it can be judged whether a sufficient amount has been collected according to the expansion degree of the sampling pad.

[0106] Insert the sample collector that has collected a sufficient amount of sample into the storage cavity 6 of the detection box, and the first buckle 9 in the storage cavity is buckled with the groove at the front end of the collector. The sample collector 200 is pressed down with the first buckle 9 as a fulcrum, and the connecting part 202 of the collector is embedded in the groove 12 of the rear enclosure. When the sampling pad 204 of the collector 200 is completely pressed flat on the extrusion plate 7, the connecting part 202 is snapped into the second buckle and confined in the second buckle slot. At this time, the operator can release the collector, and the collector can be kept in the extruded state.

[0107] Part of the liquid sample squeezed out from the collector sampling pad reaches the sample pad of the test paper through the sampling port 3, and the other part of the liquid sample enters the liquid storage tank of the lower plate through the guide groove 8 or the leakage hole 16, and flows into the sample pad through the cutout 20 of the test paper storage groove.

[0108] The liquid sample passes through the sample pad 102, the marker binding pad 103, and the test pad 104 to reach the absorbent pad 105. If the test pad only shows color at the C line position, it means that the sample is negative. If both the C line and the T line position show color, it means that the sample is positive and further nucleic acid analysis is required. If no color appears on the C line, it means that this test is invalid.

[0109] Example 3 Portable drug detection device and use

[0110] Drug test strips 100 are also known as drug of abuse test strips. "Drugs of abuse" (DOA) are drugs used for non-medical purposes (usually for psychedelic effects). Abuse of such drugs may lead to physical and mental harm and (in some cases) dependence, addiction, and even death. Examples of DOA include cocaine, amphetamines (e.g., black beauties, white bennies, amphetamine pills, dextroamphetamine drugs, dexies, beans), methamphetamines (crank, methamphetamine, crystal, speed), barbiturates (valium), and sedatives. Roche Pharmaceuticals, Nutley, New Jersey), sedatives (i.e., sleeping pills), lysergic acid diethylamide (LSD), tranquilizers (downers, goofballs, barbs, blue devils, yellow jackets, ludes), tricyclic antidepressants (TCAs, such as imipramine, amitriptyline, and doxepin), phencyclidine (PCP), tetrahydrocannabinol (THC, pot, dope, hash, weed, etc.), and opiates (e.g., morphine, opium, cocaine, heroin, oxycodone).

[0111] This embodiment takes a portable amphetamine (urine) colloidal gold detection device as an example.

[0112] The portable colloidal gold detection device for amphetamine (urine) comprises a test strip, a test box and a sample collector. The test strip is a portable colloidal gold detection strip for amphetamine (urine) and comprises a base card, on which a sample pad, a marker binding pad, a test pad and a water-absorbing pad are adhered and stacked one on the other from upstream to downstream. The principle of the monoclonal antibody competitively binding amphetamine conjugate and amphetamine that may be contained in urine is adopted. The marker binding pad comprises an anti-amphetamine monoclonal antibody (colloidal gold antibody) labeled with colloidal gold, and the T line of the test pad comprises an amphetamine conjugate.

[0113] During the test, the sample collector is inserted into the test subject's urine, and then the urine sample on the collector is squeezed into the test box, and the urine sample is then chromatographed upward on the test paper under the capillary effect. If the concentration of amphetamine in the urine sample is lower than 1000ng / ml, the colloidal gold antibody cannot completely bind to amphetamine. In this way, the colloidal gold antibody will be bound to the amphetamine conjugate fixed on the test pad during the chromatography process, and a purple-red strip will appear on the T line. If the concentration of amphetamine in the urine sample is higher than 1000ng / ml, the colloidal gold antibody will completely bind to amphetamine, so that the purple-red strip will not appear in the T line area due to the competitive reaction. Negative urine samples will have a purple-red strip on the T line due to the lack of antibody-antigen competitive reaction during the test process. Regardless of whether amphetamine is present in the urine sample, a purple-red strip will appear at the quality control line C line. The purple-red band in the quality control area (C) is the standard for judging whether there is enough urine sample and whether the chromatography process is normal. It also serves as the internal control standard of the reagent.

[0114] In an embodiment of using one detection device for multiple drug abuse detection, a plurality of test paper storage slots are provided on the lower plate of the detection box for placing test papers for different detection items.

[0115] Example 4 Portable Immunofluorescence Assay (FIA) Detection Device and Use

[0116] The test strip in this embodiment takes time-resolved immunofluorescence detection based on phosphorescence technology as an example.

[0117] The test strip is used to detect IgG antibodies produced after infection by pathogenic microorganisms, and the detection mode is an indirect method mode. The preparation method of the test strip includes: labeling anti-human immunoglobulin IgG antibodies with phosphorescent materials and fixing them on the marker conjugate pad; fixing the pathogenic microorganism antigen to be analyzed on the detection line of the test pad, and fixing IgG on the quality control line of the test pad.

[0118] During the test, squeeze the liquid sample on the collector into the test box, then pull out the collector, insert the test box containing the test paper described in this example into the fluorescence analyzer, and analyze the test results through the phosphorescence signal intensity values ​​of the T line and C line of the test paper. When the test line and the quality control line both produce phosphorescence signals at the same time, it is a positive reaction result, indicating that the sample contains the target analyte; when the test line does not produce a phosphorescence signal but the quality control line produces a phosphorescence signal, it is a negative reaction result, indicating that the sample does not contain the target analyte.

[0119] The detection device of the present invention can be used in the fields of disease diagnosis, drunk driving test, drug use, hormone status analysis, etc., meeting the requirements of rapid sampling and rapid detection. The sample types that can be detected by the detection device of the present invention can be urine, saliva, sample lysate, etc.

Claims

1. A detection kit, characterized in that: The detection box includes a storage cavity, a bottom plate is provided in the storage cavity, a sample inlet is provided on the bottom plate, an enclosure is provided on the periphery of the storage cavity, a hole is provided at the lower part of the enclosure or at the connection between the bottom plate and the enclosure, and the hole is connected to the interior of the detection box.

2. The detection kit according to claim 1, characterized in that: The detection box includes an observation window for observing the detection results, the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure, or the hole is arranged at the lower part of at least one side enclosure.

3. The detection kit according to claim 1, characterized in that: The detection box includes an observation window for observing the detection results, the enclosure includes a front enclosure, a rear enclosure opposite to the front enclosure, and two side enclosures connecting the front and rear enclosures, wherein the front enclosure is closer to the observation window than the rear enclosure, and the hole is arranged at the lower part of the rear enclosure and the lower part of at least one side enclosure.

4. The detection kit according to claim 2 or 3, characterized in that: The holes include a guide groove arranged at the connection between the bottom plate and the rear enclosure, a drainage hole arranged at the lower part of the rear enclosure, and a side drainage hole arranged at the lower part of at least one side enclosure, or the holes include at least one of the guide groove, the drainage hole and the side drainage hole.

5. The detection kit according to claim 2 or 3, characterized in that: The hole comprises a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure, and the guide groove and the drainage hole are separated by an isolation block.

6. The detection kit according to claim 2 or 3, characterized in that: The hole includes a guide groove arranged at the connection between the bottom plate and the rear enclosure and a drainage hole arranged at the lower part of the rear enclosure. The guide groove is connected to the drainage hole to form an "L"-shaped opening.

7. The detection kit according to claim 1, characterized in that: The storage cavity includes a first buckle.

8. The detection kit according to claim 7, characterized in that: The first buckle is arranged on the bottom plate between the injection port and the wall of the receiving chamber, and a certain interval space is left between the first buckle and the wall of the receiving chamber.

9. The detection kit according to claim 7, characterized in that: The first buckle and the hole are respectively located on two opposite sides of the injection port on the bottom plate.

10. The detection kit according to claim 1, characterized in that: Includes a test strip located inside the test box.

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