Fluorescent paper chip for detecting escherichia coli O157: H7 as well as preparation method and application of fluorescent paper chip
By modifying molecularly imprinted fluorescent nanoparticles with E. coli O157:H7 as the template on the paper chip, and using the change in fluorescence quenching rate for detection, the existing detection methods are solved, and the rapid, high specificity and quantitative detection effects are achieved.
CN119716050APending Publication Date: 2025-03-28NANJING NORMAL UNIVERSITY
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Patent Information
- Application Number
- CN202411818633.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
The existing E. coli O157:H7 detection method has problems such as long time, high labor consumption, requiring antibodies or aptamers, many interference factors, poor repetition and high price.
Method used
Molecularly imprinted fluorescent nanoparticles with E. coli O157:H7 as the template were designed and synthesized by molecular imprinting technology, and these nanoparticles were modified on the surface of paper chips, and quickly identified by changes in fluorescence quenching rate.
Benefits of technology
The rapid, high specificity and quantitative detection of E. coli O157:H7 detection simplifies the analysis process, reduces costs, improves detection efficiency and sensitivity, and has the advantages of reuse.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the field of food safety detection, and particularly discloses a fluorescent paper chip for detecting Escherichia coli O157: H7 and a preparation method and application thereof.Escherichia coli serves as a research object, molecularly imprinted fluorescent nanoparticles with Escherichia coli O157: H7 as a template are designed and synthesized through a molecular imprinting technology, and the fluorescent paper chip is used for detecting Escherichia coli O157: H7. After the surface of the paper chip is modified with the molecularly imprinted fluorescent nanoparticles, an Escherichia coli template can be sensitively and quickly recognized according to the change of fluorescence quenching rate, so that the paper-based array chip capable of specifically recognizing Escherichia coli O157: H7 is prepared, and the paper-based array chip has the characteristics of simplicity, low cost, real-time property and the like.
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