Long-time-sequence microorganism enrichment device and application method thereof

Abstract

The invention discloses a long-time-sequence microorganism enrichment device which comprises a sampling cup group, a planetary gear transmission assembly, a telescopic butt joint assembly and a water pumping assembly, the sampling cup group consists of a plurality of independent sampling cup units and is arranged on the dividing plate; a plurality of docking ports are formed in the inner side and the outer side of the index plate at fixed intervals, and each docking port is communicated with each sampling cup unit; the dividing plate can rotate around the circle center under the driving of the planetary gear transmission assembly; the telescopic butt-joint assembly drives the sliding block to move, the sliding block is provided with a support, and the two ends of the support are provided with self-centering units which can be in butt joint with butt-joint openings in the index plate; the non-butt-joint sides of the two self-centering units are each connected with a water pumping assembly. Through the configuration of the plurality of independent sampling cup units, a larger batch of samples can be treated at the same time, and the sample treatment capability is improved; meanwhile, the units are arranged at equal intervals in a staggered manner, so that impurities can be more efficiently removed, the filtering precision is improved, and the sample purity is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of bioengineering, and specifically relates to a long-time-sequence microorganism enrichment device and an application method thereof. Background Art

[0002] With the continuous progress of biotechnology and environmental science, the demand for microbial enrichment devices in scientific research, environmental monitoring, marine research and industrial applications is increasing. Microbial enrichment technology is widely used in many fields such as environmental governance, wastewater treatment, food safety testing and biopharmaceuticals, especially in marine ecology, environmental monitoring and industrial bioreactors.

[0003] Currently, the microbial enrichment devices on the market are mainly based on traditional filtration, concentration and separation methods. Most existing devices use simplified physical filtration and concentration principles when processing microbial samples. Common technologies include membrane filtration, centrifugal sedimentation, adsorption and other methods.

[0004] However, the existing microbial enrichment devices also have some disadvantages that cannot be ignored:

[0005] 1. Limited processing capacity: Many existing devices are only suitable for processing small batches of samples and cannot process large-scale samples, and cannot meet the needs of high-throughput and high-efficiency microbial enrichment applications.

[0006] 2. Maintenance and life issues: Currently, most devices have a simple structure and lack modular design. The equipment is easily damaged and difficult to maintain. Especially when used in harsh environments (such as deep-sea exploration), the equipment has poor reliability and cannot guarantee long-term stable operation.

[0007] 3. Lack of flexibility and precision: Most existing devices fail to effectively solve the accuracy issues of liquid injection, filtration and sample extraction during microbial enrichment. Due to insufficient operation precision, uneven sample processing and unstable enrichment effects may occur, which in turn affects the accuracy of experimental results.

[0008] In summary, although the existing technology can meet the basic needs of microbial enrichment, it still has significant deficiencies in processing large-scale samples, improving enrichment efficiency, and optimizing equipment performance. Summary of the invention

[0009] The main purpose of the present invention is to provide a long-time microorganism enrichment device which is easy to replace and has high operating accuracy, and an application method thereof.

[0010] The long-time microorganism enrichment device provided by the present invention comprises a sampling cup group, a planetary gear transmission component, a telescopic docking component and a water pumping component; the sampling cup group is composed of a plurality of independent sampling cup units, which are arranged on a dividing plate; a plurality of docking ports are arranged at fixed intervals on the inner side and the outer side of the dividing plate, and each docking port is connected to each sampling cup unit; the dividing plate can rotate along the center of a circle driven by the planetary gear transmission component; the telescopic docking component drives the slider to move, a bracket is arranged on the slider, and self-centering units are arranged at both ends of the bracket, which can be docked with the docking ports on the dividing plate; the non-docking sides of the two self-centering units are each connected to a water pumping component.

[0011] In one embodiment of the above device, the sampling cup units on the indexing plate are staggered and arranged at equal intervals, and the sampling cup units have independently opened cup covers.

[0012] In one embodiment of the above device, the sampling cup unit is provided with two large and small pipes, which are respectively connected to the 24 joint and the 10 joint on the top surface of the dividing plate.

[0013] In one embodiment of the above-mentioned device, the planetary gear transmission assembly includes a base, a tray, a rotating disk support ring, a planetary gear pair and a dividing plate; the base is a circular thick base, the tray is an identical circular disk, and the tray is supported above the base by a plurality of support rods arranged at intervals along the circumference; a rotating disk support ring is provided on the tray, and the upper and lower end surfaces of the rotating disk support ring can rotate relatively along the center of the circle, and the lower end surface of the rotating disk support ring is fixed to the tray; the planetary gear pair includes an outer ring gear ring and a cylindrical gear that are meshed with each other, the outer ring gear ring is fixed to the upper end surface of the rotating disk support ring, the bottom end of the cylindrical gear is connected to the output shaft of the rotating motor, and the rotating motor is fixed to the base; the dividing plate is fixed to the outer ring gear ring.

[0014] In one embodiment of the above device, a group of docking ports and plugs are arranged in a circumferential manner with equal spacing on the outer side of the dividing plate, and a group of docking ports are arranged in a circumferential manner with equal spacing on the inner side. The two groups of docking ports are of the same height and are both located above the plugs; the outer and inner docking ports are respectively connected to the 24 joints and 10 joints on the top surface of the dividing plate in a one-to-one correspondence.

[0015] In one embodiment of the above-mentioned device, the telescopic docking assembly includes a sliding seat, a motor, a screw, a bracket and a self-centering unit; the sliding seat includes two opposing ear seats, four limit rods are arranged between the two ear seats, the four limit rods pass through a slider, and the slider can slide on the limit rods; the motor is composed of a stepper motor and a harmonic reducer; the output shaft of the motor is connected to a coupling, and the end of the coupling is connected to a ball screw; the screw is inserted into the center of the slider and meshes with it; the bracket includes a U-shaped plate and a high-strength vertical plate perpendicular to the two ends of the U-shaped plate; the bottom center of the U-shaped plate is fixedly connected to the slider; a self-centering unit is arranged on each of the two vertical plates.

[0016] In one embodiment of the above device, a telescopic compensation gasket, a pressure compensation membrane and an oil filling port are provided inside the rear cover of the motor; the oil filling port includes a plug, a plug seat and a plug nut, the plug is placed on the plug seat and connected to the rear cover through the plug nut.

[0017] In one embodiment of the above-mentioned device, the self-centering unit includes a pair of symmetrically arranged elastic ball membranes and ball head covers, which are connected to the vertical plate through the ball head covers; among the two self-centering units, one self-centering unit is docked with the inner docking port of the dividing disk, and its docking nozzle is a ball head injection nozzle; the other self-centering unit is docked with the outer docking port of the dividing disk, and its docking nozzle is a ball head filter nozzle.

[0018] In one embodiment of the above device, the water pumping assembly includes two pairs of water pump motors and flow meters arranged on their water pumping pipelines, and the two water pumping pipelines are respectively connected to the input ends of the two self-centering units.

[0019] A method for using the above-mentioned long-time microorganism enrichment device comprises the following steps:

[0020] 1. Assemble and inspect the device to ensure that the sampling cup set, pipeline docking system, planetary gear transmission assembly and telescopic docking assembly are installed in place;

[0021] 2. The starting device aligns the self-centering assembly of the sampling cup group with the corresponding interface on the indexing plate through the planetary gear transmission assembly to ensure accurate connection;

[0022] 3. The motor drives the slider to drive the bracket and the self-centering unit to move linearly, so that the ball head injection nozzle and the indexing plate can be accurately docked;

[0023] 4. After the ball head injection nozzle is connected, the water pump assembly injects seawater into the sampling cup group along the pipeline, and then repeats the seawater injection work until the sampling cup group is completely injected with seawater;

[0024] 5. After the seawater injection is completed, the motor drives in the reverse direction until the ball head filter on the self-centering assembly is accurately docked with the corresponding docking port on the indexing plate;

[0025] 6. After the ball head filter tip is connected, the water pump assembly injects the fixative into the sampling cup along the pipeline, and then repeats the fixative injection work until all the sampling cups are injected with the fixative to perform microbial filtration and enrichment work;

[0026] 7. After the biological filtration and enrichment work is completed, the lid of the sampling cup can be opened for sampling.

[0027] The beneficial effects of the present invention are as follows:

[0028] 1. The present invention can process a larger batch of samples at the same time through the configuration of multiple independent sampling cup units, thereby improving the sample processing capacity; at the same time, these units have an equidistant and staggered arrangement, which can more efficiently remove impurities and improve the filtration accuracy and sample purity;

[0029] 2. The present invention divides the device into multiple functional modules, which is convenient for daily maintenance and regular replacement of parts; this not only improves the reliability of the equipment, but also greatly prolongs its service life, and is particularly suitable for the transportation of underwater vehicles in scientific research experiments;

[0030] 3. The planetary gear device of the present invention can accurately control the movement of each sampling cup unit, and the telescopic docking system drives the movement of the bracket and the self-centering unit through a linear motor; each component makes the device more precise and efficient during operation, greatly improving the accuracy and stability of microbial enrichment. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of the axonometric structure of an embodiment of the present invention.

[0032] Figure 2 for Figure 1 Schematic diagram of the front view structure.

[0033] Figure 3 for Figure 1 Schematic diagram of the front cross-sectional structure.

[0034] Figure 4 for Figure 1 Schematic diagram of the structure of the telescopic docking assembly.

[0035] Figure 5 for Figure 4 Schematic diagram of the cross-sectional structure. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the relevant technical solutions. Obviously, the described embodiments are only part of the embodiments, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] like Figure 1 As shown, the long-time microorganism enrichment device disclosed in this embodiment includes a sampling cup set 1 and a pipeline docking system.

[0038] The sampling cup set 1 is used for enrichment, storage and impurity filtration of microorganisms; the pipeline docking system includes a planetary gear transmission component 2, a telescopic docking component 3 and a water pump component to complete the injection, filtration and discharge of liquids.

[0039] The sampling cup group 1 is composed of a plurality of independent sampling cup units, wherein each sampling cup is staggered and evenly spaced above the graduated disk to improve the filtration efficiency and sample purity; each sampling cup has an independently openable cup cover to facilitate sample extraction after the enrichment work is completed.

[0040] The sampling cup is provided with two types of pipes, large and small, which are respectively connected to the 24 joints and 10 joints on the top surface of the dividing plate to ensure the smoothness of liquid injection and discharge.

[0041] like Figure 2 and Figure 3 As shown, the planetary gear transmission assembly 2 is used to control the position and docking action of the sampling cup unit, and its components include a base 21, a tray 22, a rotating disk support ring 23, a planetary gear pair 24 and a dividing disk 25 from bottom to top according to the assembly relationship.

[0042] The base 21 is a round thick base, and the tray 22 is a same round disc, and the tray is supported above the base by a plurality of support rods arranged along the circumference at intervals. The tray is provided to provide a stable structure of the whole machine.

[0043] A rotating disk support ring 23 is provided on the tray 22. The upper and lower end surfaces of the rotating disk support ring 23 can rotate relatively along the center of a circle, and the lower end surface of the rotating disk support ring is fixed to the tray.

[0044] The planetary gear pair 24 includes an outer ring gear ring and a cylindrical gear that mesh with each other, the outer ring gear ring is fixed to the upper end surface of the rotating disk support ring, and the bottom end of the cylindrical gear is connected to the output shaft of the rotating motor, and the rotating motor is fixed on the base 21. The rotating motor can drive the cylindrical gear, thereby driving the outer ring gear to rotate.

[0045] The indexing plate 25 is fixed on the outer gear ring. The outer side of the indexing plate is provided with a group of equidistantly spaced circumferentially arranged docking ports and plugs, and the inner side is provided with a group of equidistantly spaced circumferentially arranged docking ports. The two groups of docking ports are at the same height and are both located above the plugs. The outer and inner docking ports are connected to the 24-pin and 10-pin connectors on the top surface of the indexing plate in a one-to-one correspondence.

[0046] Through the planetary gear transmission assembly, the indexing plate can be rotated accurately to match the position of the corresponding sampling cup with the pipeline docking system.

[0047] like Figure 4 and Figure 5 As shown, the telescopic docking assembly 3 includes a slide 31 , a motor 32 , a screw 33 , a bracket 34 and a self-centering unit 35 .

[0048] The sliding seat 31 includes two opposite ear seats, four limiting rods are arranged between the two ear seats, and the four limiting rods pass through a sliding block, and the sliding block can slide on the limiting rods.

[0049] The motor 32 is composed of a stepper motor and a harmonic reducer. The output shaft of the motor is connected to a coupling, and the end of the coupling is connected to a ball screw 33. The screw is inserted into the center of the slider and meshes with it. The rotation of the motor drives the coupling and the screw to rotate, and the slider can be controlled to slide along the limit rod.

[0050] A telescopic compensation gasket, a pressure compensation membrane and an oil filling port are arranged inside the rear cover of the motor 32. The oil filling port comprises a plug, a plug seat and a plug nut, wherein the plug is placed at the plug seat and connected to the rear cover through the plug nut.

[0051] The bracket 34 includes a U-shaped plate and high-strength vertical plates perpendicular to the two ends of the U-shaped plate. The center of the bottom surface of the U-shaped plate is fixedly connected to the slider.

[0052] Each of the two vertical plates is provided with a self-centering unit 35. The self-centering unit comprises a pair of symmetrically arranged elastic spherical membranes and a spherical head cover, and is connected to the vertical plate through the spherical head cover.

[0053] The difference between the two self-centering units 35 is that the self-centering unit docking with the inner docking port of the indexing disk 25 is a ball head injection nozzle, and the self-centering unit docking with the outer docking port of the indexing disk is a ball head filter nozzle, and the corresponding air pipe joints of the two have different specifications.

[0054] The water pump assembly includes two pairs of water pump motors and flow meters arranged on the water pump pipelines thereof, and the two water pump pipelines are respectively connected to the input ends of the two self-centering units 35. The water pump assembly is arranged to pump the liquid to be treated into the pipeline in the indexing plate through the self-centering assembly, and finally pump the liquid into the sampling cup set.

[0055] The application method of this device is as follows:

[0056] 1. Assemble and inspect the device to ensure that the sampling cup set, pipeline docking system, planetary gear transmission assembly and telescopic docking assembly are installed in place;

[0057] 2. The starting device aligns the self-centering assembly of the sampling cup group with the corresponding interface on the indexing plate through the planetary gear transmission assembly to ensure accurate connection;

[0058] 3. The motor drives the slider to drive the bracket and the self-centering unit to move linearly, so that the ball head injection nozzle and the indexing plate can be accurately docked;

[0059] 4. After the ball head injection nozzle is connected, the water pump assembly injects seawater into the sampling cup group along the pipeline, and then repeats the seawater injection work until the sampling cup group is completely injected with seawater;

[0060] 5. After the seawater injection is completed, the motor drives in the reverse direction until the ball head filter on the self-centering assembly is accurately docked with the corresponding docking port on the indexing plate;

[0061] 6. After the ball head filter tip is connected, the water pump assembly injects the fixative into the sampling cup along the pipeline, and then repeats the fixative injection work until all the sampling cups are injected with the fixative to perform microbial filtration and enrichment work;

[0062] 7. After the biological filtration and enrichment work is completed, the lid of the sampling cup can be opened for sampling.

[0063] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although detailed descriptions have been made with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A long-time microbial enrichment device, characterized in that: It includes a sampling cup assembly, a planetary gear transmission assembly, a telescopic docking assembly and a water pump assembly; The sampling cup group is composed of a plurality of independent sampling cup units, which are arranged on a dividing plate; a plurality of docking ports are arranged at fixed intervals on the inner side and the outer side of the dividing plate, and each docking port is connected to each sampling cup unit; The indexing plate can rotate along the center of the circle driven by the planetary gear transmission assembly; The telescopic docking assembly drives the slider to move. A bracket is arranged on the slider. Self-centering units are arranged at both ends of the bracket and can be docked with the docking ports on the dividing plate. The non-docking sides of the two self-centering units are each connected with a water pump assembly.

2. The long-term microbial enrichment device according to claim 1, characterized in that: The sampling cup units on the dividing plate are staggered and arranged at equal intervals, and the sampling cup units have independently opened cup covers.

3. The long-term microbial enrichment device according to claim 2, characterized in that: The sampling cup unit is provided with two large and small pipes, which are respectively connected to the 24 joint and the 10 joint on the top surface of the dividing plate.

4. The long-term microorganism enrichment device according to claim 3, characterized in that: The planetary gear transmission assembly includes a base, a tray, a rotating disk support ring, a planetary gear pair and a dividing disk; The base is a round thick base, and the tray is a same round tray. The tray is supported above the base by a plurality of support rods arranged along the circumference. A rotating disk support ring is provided on the tray, and the upper and lower end surfaces of the rotating disk support ring can rotate relatively along the center of the circle, and the lower end surface of the rotating disk support ring is fixed to the tray. The planetary gear pair includes an outer ring gear ring and a cylindrical gear that mesh with each other. The outer ring gear ring is fixed to the upper end surface of the rotating disk support ring. The bottom end of the cylindrical gear is connected to the output shaft of the rotating motor. The rotating motor is fixed on the base; the dividing plate is fixed on the outer ring gear ring.

5. The long-term microorganism enrichment device according to claim 4, characterized in that: The outer side of the dividing plate is respectively provided with a group of docking ports and plugs arranged in a circumferential manner with equal spacing, and the inner side is provided with a group of docking ports arranged in a circumferential manner with equal spacing. The two groups of docking ports are of the same height and are both located above the plugs; the outer and inner docking ports are respectively connected to the 24 joints and 10 joints on the top surface of the dividing plate in a one-to-one correspondence.

6. The long-term microorganism enrichment device according to claim 1, characterized in that: The telescopic docking assembly includes a slide, a motor, a screw rod, a bracket and a self-centering unit; The sliding seat comprises two opposite ear seats, four limit rods are arranged between the two ear seats, the four limit rods pass through a sliding block, and the sliding block can slide on the limit rods; The motor is composed of a stepper motor and a harmonic reducer; the output shaft of the motor is connected to a coupling, and the end of the coupling is connected to a ball screw; the screw is inserted into the center of the slider and meshes with it; The bracket comprises a U-shaped plate and high-strength vertical plates perpendicular to the two ends of the U-shaped plate; the center of the bottom surface of the U-shaped plate is fixedly connected to the sliding block; and a self-centering unit is respectively arranged on the two vertical plates.

7. The long-term microorganism enrichment device according to claim 6, characterized in that: A telescopic compensation gasket, a pressure compensation membrane and an oil filling port are arranged inside the rear cover of the motor; the oil filling port comprises a plug, a plug seat and a plug nut, the plug is placed at the plug seat and connected to the rear cover through the plug nut.

8. The long-term microorganism enrichment device according to claim 6, characterized in that: The self-centering unit includes a pair of symmetrically arranged elastic ball membranes and ball head covers, which are connected to the vertical plate through the ball head covers; among the two self-centering units, one self-centering unit is docked with the inner docking port of the dividing plate, and its docking nozzle is a ball head injection nozzle; the other self-centering unit is docked with the outer docking port of the dividing plate, and its docking nozzle is a ball head filter nozzle.

9. The long-term microorganism enrichment device according to claim 8, characterized in that: The water pump assembly comprises two pairs of water pump motors and flow meters arranged on the water pump pipelines thereof, and the two water pump pipelines are respectively connected to the input ends of the two self-centering units.

10. A method for using the long-time microorganism enrichment device according to claims 1-9, comprising the following steps: 1) Assemble and inspect the device to ensure that the sampling cup set, pipeline docking system, planetary gear transmission assembly and telescopic docking assembly are installed in place; 2) The starting device uses the planetary gear transmission assembly to align the self-centering assembly of the sampling cup assembly with the corresponding interface on the indexing plate to ensure accurate connection; 3) The motor drives the slider to drive the bracket and the self-centering unit to move linearly, so that the ball head injection nozzle and the indexing plate can be accurately docked; 4) After the ball head injection nozzle is connected, the water pump assembly injects seawater into the sampling cup group along the pipeline, and then repeats the seawater injection work until the sampling cup group is completely injected with seawater; 5) After the seawater injection is completed, the motor drives in the reverse direction until the ball head filter on the self-centering assembly is accurately docked with the corresponding docking port on the indexing plate; 6) After the ball head filter tip is connected, the pump assembly injects the fixative into the sampling cup along the pipeline, and then repeats the fixative injection until all the sampling cups are injected with the fixative to perform microbial filtration and enrichment. 7) After the biological filtration and enrichment work is completed, the lid of the sampling cup can be opened to take samples.

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