Microorganism colony culture intelligent image acquisition system and microorganism expert auxiliary analysis system

Abstract

The invention relates to the fields of biological laboratory instruments and equipment, microbiological detection and analysis instruments and the like, in particular to a microbiological colony culture intelligent image acquisition system and a microbiological expert auxiliary analysis system. The intelligent image acquisition system for microbial colony culture comprises: a loading platform; a lens of the image acquisition device is aligned with the observation sample on the first side of the object carrying platform; the transmission light source is arranged on the second side, away from the image acquisition device, of the carrying platform; the annular light source comprises an annular base; the reflected light units and the ultraviolet light units are arranged on the annular base at intervals, a plurality of reflected light units form a reflected light source, and a plurality of ultraviolet light units form an ultraviolet light source; and the control device is used for controlling the transmission light source, the reflection light source and the ultraviolet light source to be turned on and off respectively and controlling the image acquisition device to perform image acquisition on the microbial colonies. According to the invention, uniform reflection light sources and ultraviolet light sources can be provided, and the illumination intensity of the reflection light sources can be controlled.

Description

Technical Field

[0001] The present invention relates to testing and analyzing microorganisms by measuring the physical properties of microbial colonies, and in particular to the fields of biological laboratory instruments and equipment, microbial detection and analysis instruments, and specifically to an intelligent image acquisition system for microbial colony culture and a microbial expert-assisted analysis system. Background Art

[0002] The cultivation of microorganisms is a basic common technology across industries and disciplines. Different microorganisms have different nutritional support requirements for their own growth, which has led to a wide variety of microbial culture media. At the same time, different types of microorganisms have different growth morphologies on their specific culture media, which adds varying degrees of difficulty to microbial practitioners in conducting microbial testing and research. Taking the national standard for food microbiology inspection "GB 4789.28-2024 Quality Requirements for Food Microbiology Inspection Culture Media and Reagents" as an example, there are more than 40 types of solid culture media listed, and the morphology of different strains on each culture medium is different. Inspectors must not only be proficient in the types of culture media used for different test items, but also keep in mind the morphological characteristics of the pathogenic microorganisms being tested on them, so as to conduct preliminary identification and count the number of colonies. In actual work, when microbiologists interpret the colonies growing on the plate, they often turn the plate over at multiple angles facing the light or sunlight, the front and back sides, and observe the surface, back, side and other information of the colonies, and then comprehensively interpret the possible types of colonies. Especially when observing some bacteria that are transparent or translucent, or have no obvious hemolysis, or colonies growing on the edge of the side wall, it is difficult to make judgments from just one angle.

[0003] After the microbial culture is completed, it is necessary to manually judge the microbial colonies that need to be further studied on the culture medium plate. This process relies on the individual's knowledge of the morphological characteristics of colonies on different culture media, and requires extremely high personal experience. However, if the selection is wrong, missed detection or wrong detection may occur. If this happens in the field of microbial product development, it will cause costs to rise and R&D efficiency to decrease. If it happens in the clinical or disease prevention field, it will delay the treatment cycle and even cause the spread of the epidemic. Summary of the invention

[0004] The present invention is expected to at least partially solve one of the above technical problems.

[0005] The first aspect of the present invention provides a microbial colony culture intelligent image acquisition system. The microbial colony culture intelligent image acquisition system comprises: A loading platform, used for carrying a culture container containing an observation sample; An image acquisition device, whose lens is aimed at the observation sample on the first side of the object loading platform; A transmission light source is arranged on a second side of the object-carrying platform away from the image acquisition device; The annular light source comprises: an annular base, which is arranged between the observed sample and the image acquisition device, and whose inner diameter is larger than the outer diameter of the culture container; and reflecting light units and ultraviolet light units arranged at intervals on the annular base, the number of both of which is more than 3, wherein a plurality of reflecting light units constitute a reflecting light source, and a plurality of ultraviolet light units constitute an ultraviolet light source; The control device has a signal output end connected to the transmission light source, the light valve, the reflection light source, the ultraviolet light source and the image acquisition device, and is used to control the opening and closing of the transmission light source, the reflection light source and the ultraviolet light source, and control the image acquisition device to acquire images of the microbial colonies.

[0006] In some embodiments of the present invention, it also includes: a light valve, which is arranged between the observation sample and the transmission light source, and can controllably transmit or block the light from the transmission light source to the observation sample; a control device, whose signal output end is connected to the light valve, for controlling the opening and closing of the light valve.

[0007] In some embodiments of the present invention, the control device includes: a first storage unit, used to store N image acquisition modes for capturing images of microbial colonies, N≥2, each image acquisition mode includes: a light source mode; a main control unit, used to execute the following control logic: step D1, reading the image acquisition mode in the first storage unit; step D2, according to the light source mode in the image acquisition mode, controlling the opening and closing of the transmitted light source, the light valve, the reflected light source, and the ultraviolet light source; step D3, controlling the image acquisition device to capture images of microbial colonies; step D4, storing the microbial colony image in the second storage unit.

[0008] In some embodiments of the present invention, the first storage unit is also used to store a mapping relationship database, and the optional items for the mapping relationship in the mapping relationship database are one or more of the following: culture medium, microorganism, and observation purpose, and the dependent variable is: image acquisition mode; the main control unit is also used to execute the following control logic: step A, receiving one or more optional items of microbial colony culture; step B, searching for the image acquisition mode corresponding to the received optional items in the mapping relationship database, and recommending the image acquisition mode to the user; step C, receiving the user's choice of image acquisition mode.

[0009] The second aspect of the present invention provides a microbial expert-assisted analysis system. The microbial expert-assisted analysis system comprises: the above-mentioned microbial colony culture intelligent image acquisition system, wherein the main control unit is also used to use M different image acquisition modes in the same shooting cycle to alternately execute steps D1 to D4, M≥2; an intelligent analysis system, which uses the microbial colony image sequence obtained by the microbial colony culture intelligent image acquisition system through different image acquisition modes to perform combined analysis and annotate the microbial colony image; wherein the type of microorganism in the observed sample is unknown; the intelligent analysis system infers possible target microorganisms by combining and analyzing the microbial colony image sequence.

[0010] It can be seen from the above technical solution that the present invention has at least one of the following beneficial effects compared with the prior art: (1) Setting of reflected light source and ultraviolet light source In the present invention, a plurality of white light LED units form a reflective light source; a plurality of ultraviolet LED units form an ultraviolet light source, and all LED units are arranged on a ring lamp holder. Such an arrangement can provide a uniform reflective light source and an ultraviolet light source, and is also conducive to controlling the light intensity of the reflective light source.

[0011] (2) Invert the culture container In the present invention, the culture container is inverted, that is, the bottom is on top and the cover is down. In this case, it is not easy to generate water vapor on the cover during the culture process, which can improve the effect of microbial colony image acquisition.

[0012] (3) Switching between light and dark backgrounds through liquid crystal light valves In the present invention, by controlling the transparency of the liquid crystal light valve to create a light / dark background, a variety of background modes can be achieved. The dark background has a better shooting effect and is more conducive to observing the growth of different types of microorganisms from different backgrounds.

[0013] (4) First storage unit and second storage unit The first storage unit is a system-built storage unit, which has a fast reading speed but a small storage capacity, and is inseparably integrated with the main control unit on the same board. The second storage unit is detachably installed on the board, and its storage capacity can be expanded to meet the storage requirements of larger colony images.

[0014] The second storage unit is not limited by physical space and can be located on the same hard disk or the same device as the first storage unit, or can adopt a spatial storage method, such as a network server or a cloud server.

[0015] (5) Image acquisition mode saves experimental time and facilitates user operation According to the culture medium type, colony morphology, biochemical characteristics and background color of different plates, the required light source mode, brightness, white balance conditions, acquisition frequency, acquisition time and other shooting parameters are determined, and the light source mode and numerous shooting parameters are stored in the image acquisition mode. When the user observes the microbial colonies, the existing image acquisition mode can be directly called. On the one hand, the knowledge warehouse is solidified, and on the other hand, the experimental steps and experimental time are saved, which is more friendly to those who are first exposed to microbial experiments. Thirdly, it is quick to get started, easy for users to accept, and easier to market.

[0016] (6) Conveniently select image acquisition mode through mapping relationship In the present invention, the light source mode and shooting parameters are optimized through mapping relationships. The principle is to be able to clearly and continuously record the growth process of microorganisms while minimizing the storage space, so as to facilitate the acquisition of fixed characteristics of a specific species of microorganisms formed on the culture medium used for the isolation and cultivation of the microorganisms of the species in relevant national or international standards, thereby lowering the usage threshold, facilitating user operation, and realizing the standardization and convenience of microbial colony culture detection.

[0017] (7) Multiple image acquisition modes are used to shoot alternately. Two or more image acquisition modes are used alternately to take pictures, with the interval between two pictures being only a few seconds. This can be regarded as two light source pictures of the microbial colony morphology at the same time point. In this case, the collected microbial colony images not only have a time dimension, but also a light source mode dimension and / or a shooting mode dimension. The information obtained from the two pictures can complement or verify each other, thereby obtaining a clearer and more accurate research dimension.

[0018] (8) Traceable data with original watermark In the present invention, what is stored is not a simple microbial colony image, but the following auxiliary information is integrated with the microbial colony image as traceable data with an original watermark for storage: culture medium and / or microorganism, image acquisition mode, temperature and / or humidity, operator, image acquisition time. Through such a setting, the microbial colony image is made more three-dimensional, avoiding the loss of auxiliary information, and researchers can analyze the obtained microbial colony image more comprehensively, and it has the function of all-round traceability of microbial colony images, establishes an evidence-based system in the process of microbial research, forms a traceable research model, and enhances the rigor and reliability of microbial research.

[0019] (9) Microbiological expert-assisted analysis system In the present invention, the image information obtained by different light source modes and shooting modes continuously obtained from the same microbial plate at the same time point can be combined and analyzed, and possible target microorganisms can be obtained and labeled through multi-parameter combination to establish an expert suggestion system based on colony morphological characteristics.

[0020] (10) Multi-parameter combination improves the accuracy of microbial expert-assisted analysis system By freely combining and selecting multiple parameters, the accuracy or sensitivity of the expert-assisted system can be improved. As the number of combined parameters increases, the accuracy increases, avoiding repetitive work and false positives. As the number of combined parameters decreases, the sensitivity increases, avoiding missed detections and false negatives. This can improve the efficiency of identifying pathogenic microorganisms in clinical research and disease prevention and control, saving valuable time for patient treatment and epidemic control. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Schematic diagram of the structure of the intelligent image acquisition system for microbial colony culture according to an embodiment of the present invention.

[0022] Figure 2 for Figure 1 Schematic diagram of the control device in the intelligent image acquisition system for microbial colony culture.

[0023] Figure 3 for Figure 1 The interface diagram of the control mode setting in the microbial colony culture intelligent image acquisition system is shown.

[0024] Figure 4 These are colony images of Staphylococcus aureus collected on Columbia blood agar plates under different light source modes at the same time point.

[0025] Figure 5 These are colony images of Staphylococcus aureus grown on a BP plate collected in time series under reflected light dark background mode. DETAILED DESCRIPTION

[0026] The present invention provides a solution for shooting microbial colony growth sequences in multiple image acquisition modes. Specifically, by selecting corresponding light source modes and shooting modes, the characteristics of microorganisms on different culture media are made more obvious, artificial intelligence is used for identification and extraction, and an expert-assisted analysis system for microbial separation and identification is established, thereby reducing the difficulty of scientific researchers in exploring experimental parameters during the experiment and achieving standardization and convenience of microbial colony image acquisition.

[0027] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific implementation methods and with reference to the accompanying drawings.

[0028] Figure 1 Schematic diagram of the structure of the intelligent image acquisition system for microbial colony culture according to an embodiment of the present invention. Figure 1 As shown, the microbial colony culture intelligent image acquisition system of this embodiment includes: A loading platform, used for carrying a culture container 11 containing an observation sample; An image acquisition device 20, whose lens is aimed at the observation sample on the first side of the object loading platform; a temperature sensor and / or a humidity sensor, the sensing contacts of which extend into the chamber where the culture dish is located; A transmission light source 30 is disposed on a second side of the object-carrying platform away from the image acquisition device; The light valve 40 is disposed between the observation sample and the transmission light source, and can controllably transmit or block the light emitted from the transmission light source to the observation sample; The annular light source 50 comprises: an annular base, which is arranged between the observed sample and the image acquisition device, and whose inner diameter is larger than the outer diameter of the culture container; and reflective light units and ultraviolet light units arranged at intervals on the annular base, the number of both units being more than 3, wherein a plurality of reflective light units constitute a reflective light source, and a plurality of ultraviolet light units constitute an ultraviolet light source; The control device 60 has a signal output end connected to the transmitted light source, light valve, reflected light source, ultraviolet light source and image acquisition device, and is used to control the opening and closing of the transmitted light source, light valve, reflected light source and ultraviolet light source, and control the image acquisition device to perform image acquisition.

[0029] The following is a detailed description of each part of the microbial colony culture intelligent image acquisition system of this embodiment.

[0030] In this embodiment, the culture container 11 is a flat dish. The transmission light source 30 and the light valve 40 are located on one side of the culture container 11, and the annular light source 50 and the image acquisition device 20 are located on the other side of the culture container.

[0031] Regarding the culture container, light source, light valve, and control device, the following aspects need to be explained: (1) Invert the culture container for image acquisition In the prior art, when collecting images of microbial colony cultures, the culture container is placed upright, that is, the cover is on top and the bottom is on the bottom. However, in practice, the applicant has found that the dish cover often becomes blurred due to water vapor.

[0032] Different from the prior art, in this embodiment, the culture container 11 is inverted, that is, the bottom is on top and the cover is down. In this case, it is not easy to generate water vapor on the cover during the culture process, which can improve the effect of microbial colony image acquisition.

[0033] In this embodiment, the culture container is inverted as a preferred solution, but the culture container is upright and is also within the scope of the present application.

[0034] (2) Transmitted light source uses LED flat panel The transmitted light source 30 adopts an LED flat panel, which has a more uniform light source and a better image effect compared to lamp beads.

[0035] (3) Using liquid crystal light valve to switch between bright and dark backgrounds In the prior art, a dark background is usually obtained by turning off the transmitted light source, but the applicant has found that the dark background image obtained in this way is not ideal.

[0036] In this embodiment, the light valve 40 is disposed between the observation sample and the transmission light source and is controlled by the control device. The light valve 40 can block the light from the transmission light source from irradiating the observation sample.

[0037] Preferably, the light valve 40 is a liquid crystal light valve, and the horizontal extension range of the liquid crystal light valve is greater than the horizontal extension range of the flat light source. By controlling the transparency of the liquid crystal light valve to create a light / dark background, a variety of background modes can be achieved, and the dark background shooting effect is better, which is more conducive to observing the growth of different types of microorganisms from different backgrounds. Furthermore, the liquid crystal light valve can be a positive liquid crystal light valve or a negative liquid crystal light valve.

[0038] (4) The UV LED lamp beads and white light LED lamp beads are arranged alternately on the ring base, providing conditions for realizing UV image acquisition In the prior art, it is impossible to realize ultraviolet imaging of microbial colony formation. How to add an ultraviolet light source when there are a transmitted light source and a reflected light source is a difficult problem that troubles technicians in this field.

[0039] In this embodiment, the annular light source 50 adopts a staggered arrangement of white light LED lamp beads and ultraviolet LED lamp beads, and the number of both is greater than 10. A plurality of white light LED lamp beads constitute a reflective light source; a plurality of ultraviolet LED lamp beads constitute an ultraviolet light source. Such an arrangement can provide a uniform reflective light source and ultraviolet light source, and is also conducive to controlling the light intensity of the reflective light source.

[0040] (5) The combination of multiple light sources and light valves realizes a variety of lighting modes Compared with the prior art, in addition to the transmission light source and the reflection light source, this embodiment also has an ultraviolet light source and a bright field / dark field background in the light source setting, which can meet the shooting requirements of the colony morphology of different strains and is more conducive to observing the growth process of microorganisms. For example, when used for colony counting, a transmission light source can be used; when observing the surface morphology of the colony, a reflection light source can be used; and some bacteria can produce red, green, blue and other fluorescence phenomena under ultraviolet light.

[0041] (6) Temperature and humidity sensor In this embodiment, the temperature sensor and humidity sensor have sensing contacts extending into the chamber where the culture dish is located, so that the temperature and humidity information of the microbial culture can be obtained. This information will be integrated with the microbial colony image to form traceable information.

[0042] (7) Control device In this embodiment, the control device 60 is designed based on the Raspberry Pi development board, and the transmission light source, the light valve, the reflection light source, and the ultraviolet light source are controlled through the GPIO port of the Raspberry Pi development board.

[0043] Those skilled in the art should understand that in addition to the Raspberry Pi development board, other types of development boards or single-chip microcomputer technologies can also be used to realize the control of each light source and light valve, and the control interface can also be selected as needed. These variations can also implement the present invention and are also within the protection scope of the present invention.

[0044] Figure 2 for Figure 1 The schematic diagram of the control device in the microbial colony culture intelligent image acquisition system is shown in FIG. Figure 2 As shown, in this embodiment, the control device includes: a first storage unit and a main control unit. The first storage unit is used to store the image acquisition mode database and the mapping relationship database.

[0045] In this embodiment, there are both an image acquisition mode database and a mapping relationship database, and the image acquisition database is the basis of the mapping relationship database. Accordingly, this embodiment can not only realize the function of image acquisition mode selection, but also realize the function of image acquisition mode intelligent recommendation.

[0046] Those skilled in the art should understand that the above are only preferred embodiments of the present invention, but the present invention is not limited thereto. In other embodiments of the present invention, there may be only an image acquisition mode database, in which case the basic image acquisition mode selection function may also be implemented, which is also within the protection scope of the present invention.

[0047] Figure 3 for Figure 1 The interface diagram of the control mode setting in the microbial colony culture intelligent image acquisition system is shown in FIG. Figure 3 As shown, the image acquisition mode database stores N image acquisition modes for collecting microbial colony images, where N≥2. Each image acquisition mode includes: a light source mode and a shooting mode. Among them: (1) Light source mode The light source mode is a setting for the transmission light source, reflection light source, ultraviolet light source, and bright field / dark field background, the purpose of which is to provide the best shooting illumination for the current microbial colony image. Specifically, the light source mode is selected from the following modes: ① Transmission light source mode: the transmission light source is turned on, the light valve is opened, and the light emitted by the transmission light source is emitted to the observed sample through the light valve, presenting a bright background of the observed sample; the reflection light source and ultraviolet light source are turned off; ② Reflection light source dark background mode: the transmission light source is turned on, the light valve is closed, and a dark background of the observed sample is presented; the ultraviolet light source is turned off; and the reflection light source is turned on; ③ Reflection light source bright background mode: the transmission light source is turned on, the light valve is opened, and a bright background of the observed sample is presented; the ultraviolet light source is turned off; and the reflection light source is turned on; ④ Integrated light source mode, the transmission light source is turned on, the light valve is closed; the reflection light source is turned on; the ultraviolet light source is turned off; ⑤ The first ultraviolet light mode, the transmission light source is turned off, the light valve is closed, and a black background of the observed sample is presented; the reflection light source is turned off, and the ultraviolet light source is turned on; ⑥ The second ultraviolet light mode, the transmission light source is turned off, the light valve is closed, and a black background of the observed sample is presented; the reflection light source and the ultraviolet light source are turned on.

[0048] (2) Shooting mode The shooting mode mainly includes the settings of camera-related parameters, including one or more of the following settings: camera mode (exposure time, in ms), white balance setting, acquisition frequency, and acquisition duration. Different culture media have different colors and transmittances, so the camera mode and white balance settings required for shooting are also different. In addition, due to the different growth rates and growth times of different microorganisms, the acquisition frequency and acquisition duration for shooting are also different.

[0049] Based on the above hardware, in this embodiment, three kinds of working logic are set according to user needs: free selection logic, mode self-selection logic, and mode recommendation logic. Regarding the free selection logic, the user freely selects the specific values ​​of each light source, the opening / closing of the light valve, and each parameter of the shooting mode. This is the same as the existing technology and will not be repeated here. The mode self-selection logic and mode recommendation logic are described in detail as follows.

[0050] (1) Mode selection logic The first storage unit stores an image acquisition mode database.

[0051] The user does not know the specific type of microbial colony, or the user already knows which specific image acquisition mode to use. In this case, the user can choose to let the main control unit execute the mode self-selection logic. Specifically, the mode self-selection logic includes: Step C, receiving a user's selection of an image acquisition mode; Step D, performing image acquisition according to the image acquisition mode, specifically: Step D1, reading the image acquisition mode in the first storage unit; Step D2, according to the light source mode in the image acquisition mode, control the opening and closing of the transmission light source, the light valve, the reflection light source, and the ultraviolet light source; according to the shooting mode in the image acquisition mode, set parameters for the image acquisition unit; Step D3, controlling the image acquisition device to acquire images of the microbial colonies; Step D4, storing the microbial colony image in a second storage unit.

[0052] In the prior art, all microbial plates use the same light source settings and shooting modes, or the experimenters set the light source parameters and shooting parameters one by one according to the culture medium and microbial type of the current plate based on their own experience. On the one hand, such experience requires self-exploration or word of mouth, and the knowledge accumulation is poor; on the other hand, manual re-setting is required each time, and the experimental steps are relatively cumbersome.

[0053] In the present invention, a set of growth characteristic parameters of different microorganisms on specific plates is summarized, and a set of image acquisition modes that can clearly obtain prediction information is formulated based on the growth phenomenon of different microorganisms, and a combination option of different image acquisition modes is provided. Specifically, the required light source mode, brightness, white balance conditions, acquisition frequency, acquisition time and other shooting parameters are determined for the culture medium type, colony morphology, biochemical characteristics, and background color of different plates, and the light source mode and a large number of shooting parameters are stored in the image acquisition mode. When the user observes the microbial colony, the existing image acquisition mode can be directly called. On the one hand, the solidification of the knowledge warehouse is realized, and on the other hand, the experimental steps and experimental time are saved, which is more friendly to people who are first exposed to microbial experiments; thirdly, the speed of getting started is fast, users are easy to accept, and market promotion is easier.

[0054] (2) Mode recommendation logic For a specific culture medium / microorganism combination, a large number of preliminary experiments are required to explore the relevant experimental conditions. In the present invention, the system has a built-in mapping relationship database when it leaves the factory, or the user adds a new mapping relationship to the mapping relationship database in subsequent use. The mapping relationship is: the mapping relationship between culture medium and / or microorganism and / or, and image acquisition mode. The system can recommend an image acquisition mode that meets the conditions to the user based on the culture medium / microorganism / observation purpose selection according to the mapping relationship database.

[0055] Those skilled in the art should understand that the principle of optimizing the light source mode and shooting parameters through these mapping relationships is to be able to clearly and continuously record the growth process of microorganisms while minimizing the storage space, so as to facilitate the acquisition of the fixed characteristics of a specific species of microorganisms formed on the culture medium used for the isolation and culture of the microorganisms of the species in the relevant national or international standards, thereby lowering the threshold for use, facilitating user operation, and realizing the standardization and convenience of microbial colony culture detection.

[0056] In this embodiment, the user knows the culture medium and microorganism type of the currently observed sample, and can choose to let the main control module execute the mode recommendation logic. Specifically, the mode recommendation logic includes: Step A, receiving information of a culture medium for culturing microbial colonies and / or microorganisms and / or observation purposes input by a user; Step B, searching for an image acquisition mode corresponding to the culture medium and / or microorganism and / or observation purpose in a mapping relationship database, and recommending the image acquisition mode to the user; Step C, receiving a user's selection of an image acquisition mode; Step D, performing image acquisition according to the image acquisition mode, specifically: Step D1, reading the image acquisition mode in the first storage unit; Step D2, according to the light source mode in the image acquisition mode, control the opening and closing of the transmission light source, the light valve, the reflection light source, and the ultraviolet light source; according to the shooting mode in the image acquisition mode, set parameters for the image acquisition unit; Step D3, controlling the image acquisition device to acquire images of the microbial colonies; Step D4, storing the microbial colony image in a second storage unit.

[0057] It can be seen that step C and step D of the mode self-selection logic and the mode recommendation logic are the same. The difference between the two is that the mode recommendation logic also includes: the user inputs the culture medium / microorganism / observation purpose information, and the system recommends the image acquisition mode.

[0058] It should be noted that both the above mode self-selection logic and mode recommendation logic involve two storage units, namely: a first storage unit for storing image acquisition modes and mapping relationship databases; and a second storage unit for storing images. In this embodiment, the first storage unit and the second storage unit are different storage units. The first storage unit is a storage unit that comes with the system. It has a fast reading speed but a small storage capacity. It is inseparably integrated with the main control unit on the same board. The second storage unit is detachably mounted on the board, and its storage capacity can be expanded to meet the storage requirements of larger colony images. However, the present invention is not limited to this. In some occasions where the image quality requirements are low and the number of pictures is small, the first storage unit and the second storage unit can also be implemented as different partitions of the same memory. Such a deformation method is also within the protection scope of the present invention.

[0059] It should also be noted that the selection of the image acquisition mode in step C can be divided into the following five cases: (1) The recommended image acquisition mode is one Step C includes: receiving a user's determination of the image acquisition mode; or, receiving a user's modification of the image acquisition mode; or, receiving a user's rejection of the image acquisition mode, and providing the user with a choice of a custom image acquisition mode; (2) The recommended image acquisition mode is multiple Step B also includes: prompting the user with information characteristics of the microbial colony image that can be obtained by each image acquisition mode; Step C includes: receiving a user's selection of a certain image acquisition mode among multiple image acquisition modes; or receiving a user's selection of alternating shooting of two or more image acquisition modes among the multiple image acquisition modes.

[0060] (3) Correction of image acquisition mode In this embodiment, specific settings of the light source mode and the shooting mode are given, especially the various parameters in the shooting mode. However, those skilled in the art should understand that during the use of the instrument, changes in the scene and the use time of the light source will affect the image acquisition effect. Therefore, the function of parameter modification is provided in this embodiment.

[0061] Specifically, step C includes: receiving user modification of certain parameters in the light source mode and / or shooting mode in the image acquisition mode, and the modified parameters form a new image acquisition mode which can be stored in the first storage unit for the main control unit to call.

[0062] (4) Customized image acquisition mode In some cases, the recommended image acquisition mode does not meet user needs. In this case, the user should be allowed to customize the light source mode and shooting mode in the image acquisition mode.

[0063] Specifically, step C includes: receiving a user's rejection of the image acquisition mode, and providing the user with a choice of a customized light source mode and a shooting mode. The user-defined image acquisition mode can be stored in the first storage unit as a basis for the subsequent main control unit to lead the image acquisition process.

[0064] (5) Increase of image acquisition modes In addition to the built-in image acquisition modes of the system, users can also organize the image acquisition modes commonly used in experiments and store them in the system according to the preset mode for direct use in subsequent experiments. Preferably, the combination of culture medium and / or microorganism can also be mapped to the image acquisition mode to facilitate subsequent experimenters to select and use.

[0065] As mentioned above, in this embodiment, the corresponding image acquisition mode is recommended according to the culture medium and / or microorganism and / or observation purpose selected by the user. Hereinafter, the culture medium and / or microorganism and / or observation purpose for initial selection are referred to as "optional items", and the image acquisition mode determined according to the "optional items" is the dependent variable. The following examples are given for different situations.

[0066] 1. One of the options is: observation purpose or microorganism type (1) The optional items are: observation purpose, and the mapping relationship includes at least one of the following; 1.1 Observation purpose: Colony surface morphology Its corresponding dependent variable is the image acquisition mode using the following light source modes: reflected light source dark background mode or reflected light source bright background mode; 1.2 Observation purpose: the density of the group Its corresponding dependent variable is the image acquisition mode using the following light source modes: transmission light source mode; It should be noted that, in this case, the image acquisition modes that satisfy the above light source modes are all image acquisition modes to be recommended.

[0067] (2) The optional items are: microbial species, and the mapping relationship includes at least one of the following; 2.1 The microorganism is Escherichia coli The corresponding dependent variable is the image acquisition mode using the following shooting mode: the acquisition frequency is 30 minutes per image, and the acquisition duration is 24 hours; 2.2 Microorganism is beta-hemolytic streptococcus The corresponding dependent variable is the image acquisition mode using the following shooting mode: the acquisition frequency is 30 minutes per image, and the acquisition duration is 48 hours; 2.3 The microorganism is Staphylococcus aureus; The corresponding dependent variable is the image acquisition mode using the following shooting mode: the acquisition frequency is 30 minutes per image, and the acquisition duration is 48 hours; 2.4 The microorganism is Aspergillus niger; The corresponding dependent variable is the image acquisition mode using the following shooting mode: continuously acquiring images with transmitted light source and reflected light source, with an interval of 5 seconds, two pictures as a group, an acquisition frequency of 2 hours per group, and an acquisition time of 120 hours.

[0068] It should be noted that, in this case, image acquisition modes that meet the above shooting modes are all image acquisition modes to be recommended.

[0069] 2. There are two optional items: microorganisms and culture media (1) The culture medium is EMB and the microorganism is Escherichia coli; The image acquisition modes corresponding to the dependent variables include: reflected light source dark background mode, camera mode between 8-12, red light white balance between 35-45, blue light white balance between 15-20; the acquisition frequency is 30 minutes per image, and the acquisition time is 24 hours; (2) The culture medium is Columbia blood agar plate and the microorganism is beta-hemolytic Streptococcus. The image acquisition modes corresponding to the dependent variable include the following two: ① Transmitted light source mode, camera mode is between 8-10, red light white balance is between 35-45, blue light white balance is between 15-20; ② In the reflected light source dark background mode, the camera mode is between 10-12, the red light white balance is between 35-45, and the blue light white balance is between 15-20; ③Integrated light source mode, camera mode is between 8-10, red light white balance is between 35-45, blue light white balance is between 15-20; In the above three image acquisition modes, the acquisition frequency is 30 minutes per image and the acquisition time is 48 hours.

[0070] 3. There are three optional items: microorganism, culture medium, and observation purpose (1) The culture medium is BP, and the microorganism is Staphylococcus aureus. 1.1 Observation purpose: transparent ring and / or precipitation ring The image acquisition modes corresponding to the dependent variables of the above self-selected items are: transmitted light source mode, camera mode between 8-10, red light white balance between 35-45, and blue light white balance between 15-20; 1.2 Observation purpose: Black heart characteristics; The image acquisition modes corresponding to the dependent variables of the above self-selected items are: reflected light source bright background mode, camera mode between 8-10, red light white balance between 35-45, and blue light white balance between 15-20; In the above two image acquisition modes, the acquisition frequency is 30 minutes per image and the acquisition time is 48 hours; (2) The culture medium is Red Bengal plate and the microorganism is Aspergillus niger; 2.1 Observation purpose: colony counting The image acquisition modes corresponding to the dependent variables of the above self-selected items are: ① Transmitted light source mode, camera mode is between 8-10, red light white balance is between 35-45, and blue light white balance is between 15-20; 2.2 Observation objectives: colony size, color, and mycelium distribution There are two image acquisition modes for the above options corresponding to the dependent variable: ① In the reflected light source dark background mode, the camera mode is between 10-12, the red light white balance is between 35-45, and the blue light white balance is between 15-20; ②Integrated light source mode, camera mode is between 8-12, red light white balance is between 35-45, blue light white balance is between 15-20; 2.3 Observation purpose: Fluorescence phenomenon The image acquisition modes corresponding to the dependent variables of the above self-selected items are: ④ UV light source mode, camera mode is between 13-15, red light white balance is between 35-45, and blue light white balance is between 15-20; In the above four image acquisition modes, the acquisition frequency is 2 hours per image and the acquisition time is 120 hours.

[0071] It can be seen that the more complete the options provided by the user, the more specific the image acquisition mode provided, the fewer contents the user needs to select and the fewer parameters to set for the experimental work, and the smaller the workload. In addition, the above acquisition time meets the relevant national standards, and the above parameter settings have been tested by the manufacturer and can meet the research needs of microbial culture photography.

[0072] It needs to be explained again that the above determination of dependent variables based on self-selected items is only an example. Those skilled in the art can freely choose self-selected items from the three items of culture medium, microorganism, and observation purpose. The system automatically matches the relevant image acquisition mode based on the self-selected items, which will not be repeated here.

[0073] As described above, in step B, when the culture medium and / or microorganism and / or observation purpose correspond to multiple image acquisition modes, the user is prompted with information characteristics of the microbial colony image that can be obtained by each image acquisition mode.

[0074] As described in the previous two application scenarios of "the microorganism is beta-hemolytic Streptococcus and the culture medium is Columbia blood plate" and "the microorganism is Aspergillus niger and the culture medium is Bengal red plate", some combinations of culture medium + microorganism correspond to multiple image acquisition modes. In this case, step C includes the following two cases: ①The user selects one of the image acquisition modes When the user selects one of the image acquisition modes, the selected image acquisition mode is subsequently used to perform image acquisition according to the time series dimension.

[0075] ②Users select two or three acquisition modes When the user selects two or more image acquisition modes, the microbial colony culture intelligent image acquisition system will use different image acquisition modes to alternately shoot and acquire images. The interval between shots is only a few seconds, which can be regarded as two light source images of the microbial colony morphology at the same time point.

[0076] In the present invention, there are two implementation methods for the user to select two or three acquisition modes, specifically: image acquisition mode dimension and time series dimension. The image acquisition of the two dimensions is described below respectively.

[0077] 1. Image acquisition mode dimensions The existing technologies mostly use a single light source and a single shooting mode, which can only obtain partial information and cannot provide sufficient information to support bacterial species identification. In the present invention, through the acquisition of sequential light source images in different image acquisition modes, a variety of information about the colony can be comprehensively obtained to perform more comprehensive bacterial species identification. Specifically, the information obtained from the two pictures can complement or verify each other, thereby obtaining a clearer and more accurate research dimension. In the implementation process, there are the following two situations: (1) The main control unit is further used to adopt M different image acquisition modes in the same shooting cycle and alternately execute steps D1 to D4, 2≤M≤N; (2) The main control unit is further used to use different image acquisition modes to acquire images of the microbial colony in the T1th and T2th shooting cycles among T shooting cycles, T≥10, T1≠T2.

[0078] Figure 4 These are colony images of Staphylococcus aureus collected on Columbia blood agar plates under different light source modes at the same time point. Figure 4In the figure, (A) was taken in the transmitted light mode, from which the hemolysis ring of Staphylococcus aureus on the Columbia blood plate can be clearly observed, but the colony morphology cannot be seen; (B) and (C) were taken in the integrated light mode and the reflected light dark background mode respectively, and both can observe that Staphylococcus aureus on the Columbia blood plate is a pale yellow round colony with regular edges and a moist surface, but the hemolysis ring cannot be clearly seen. In comparison, the colony morphology taken in the reflected light dark background mode in (C) is clearer.

[0079] pass Figure 4 It can be seen that by taking microbial images through different image acquisition modes and comparing the images taken by multiple light source modes at the same time point, the colony information can be comprehensively obtained from multiple angles, including the transmission light source uniformly penetrating the colony to form a projection, and clearly observing the hemolytic ring, transparent circle and other phenomena; the reflected light source hits the colony surface, and the surface morphology of the colony is observed, such as roughness or smoothness, dryness or moisture, and the presence or absence of metallic luster; the colony fluorescence phenomenon is observed under the excitation of ultraviolet light source of appropriate wavelength.

[0080] For another example, when Staphylococcus aureus is identified on a blood plate, the blood plate is opaque, and the hemolysis of the colonies obtained by the transmitted light source is very obvious, but the colony morphology is almost unobservable. The surface light of the reflected light source with a dark background can obtain the colony morphology of Staphylococcus aureus as a round colony with a light yellow color and a smooth surface, but the hemolysis phenomenon is not easy to see. By adopting the technical solution of the present invention, through the combination of the transmitted light source mode and the reflected light source dark background mode, the images of the transmitted light source mode and the reflected light source dark background mode can be collected at the same time, so that the growth of the colony can be more clearly understood.

[0081] For another example, gentamicin culture medium is a culture medium commonly used in physical examinations and is used to detect cholera in intestinal outpatient clinics. The culture medium is a transparent colorless plate, and Vibrio cholerae appears as translucent, slightly gray-green, flat and slightly raised colonies on it. When photographed with a reflected light source, the color of the colonies can be observed in the bright field background, and the dark field background mostly reflects the translucent and flat and slightly raised state of the colonies. Therefore, by adopting the technical solution of the present invention, more colony information can be obtained for strain identification through the combination of the bright background mode of the reflected light source and the dark background mode of the reflected light source.

[0082] It can be seen that through the method of the present invention, at the same time point, by shooting microbial colonies with different light source modes and different backgrounds, various information on the growth of microbial colonies can be obtained.

[0083] 2. Time Series Dimension Specifically, at each time point, colony images under multiple light source modes are collected in sequence (the interval between taking photos under each light source at the same time point can be ignored), forming multiple sets of time series images under different light sources based on the microorganisms on a plate.

[0084] Figure 5 The colony images of Staphylococcus aureus grown on the BP plate were collected in time series under the dark background mode of the reflected light source. Among them, (A) is the colony image at the beginning. (B) is the colony image after 13 hours of growth. It can be seen that colonies visible to the naked eye began to grow after 13 hours of growth. (C) is the colony image after 16 hours of growth. It can be seen that the typical colony morphology can be clearly seen after 16 hours of growth: black colonies, precipitation rings surrounding the colonies, and transparent rings outside the precipitation rings. (D) is the colony image after 23 hours of growth. It can be seen that the various features shown in (C) are more obvious.

[0085] Depend on Figure 5 It can be seen that in this embodiment, by comparing the time series images under the same light source mode, the changes in the number of colonies and colony characteristics (colony diameter, colony growth rate, colony color, halo around the colonies, etc.) are obtained to accurately distinguish impurities and colonies.

[0086] Depend on Figure 4 and Figure 5 It can be seen that through comparative analysis of the two dimensions of light source mode and time series, more comprehensive colony information can be obtained compared to the existing technology, and accurate identification of colonies can be achieved.

[0087] In addition, it should be noted that this embodiment does not store a simple microbial colony image, but integrates one or more of the following auxiliary information with the microbial colony image as traceable data with an original watermark for storage: culture medium and / or microorganism, image acquisition mode, temperature and / or humidity, operator, image acquisition time. Through such a setting, the microbial colony image is made more three-dimensional, avoiding the loss of auxiliary information, and researchers can analyze the obtained microbial colony image more comprehensively, and it has the function of all-round traceability of research results, which enhances the rigor and reliability of microbial research.

[0088] The second aspect of the present invention provides a microbial expert-assisted analysis system. As mentioned above, through the microbial colony culture intelligent image acquisition system of the above embodiment, the present invention can obtain colony images taken under multiple image acquisition modes at the same time point or at different time points, and obtain information such as colony morphology, colony or bacterial core color, surface smoothness, hemolysis, metallic luster, transparent ring, precipitation ring, colony growth rate, and bacterial emergence time for comprehensive judgment for bacterial species identification and observation.

[0089] The microbial expert-assisted analysis system of an embodiment of the present invention includes: the above-mentioned microbial colony culture intelligent image acquisition system, wherein the type of microorganisms in the observed sample is unknown; the main control unit is also used to use M different image acquisition modes in the same shooting cycle to alternately execute steps D1 to D4, M≥2; an intelligent analysis system, which uses the microbial colony image sequences obtained by the microbial colony culture intelligent image acquisition system through different image acquisition modes to perform combined analysis, infer possible target microorganisms, and annotate the microbial colony images.

[0090] In this embodiment, the image information obtained by different light source modes and shooting modes continuously obtained at the same time point on the same microbial plate can be combined and analyzed, and possible target microorganisms can be obtained through multi-parameter combination and labeled to establish an expert-assisted analysis system based on colony morphological characteristics. Furthermore, by freely combining and selecting the obtained multi-parameters, the accuracy or sensitivity of the expert-assisted system can be improved. As the combined parameters increase, the accuracy is improved, avoiding repetitive work and false positives. As the combined parameters decrease, the sensitivity is improved, avoiding missed detections and false negatives. This can improve the efficiency of identifying pathogenic microorganisms in clinical research and disease prevention and control, saving valuable time for patient treatment and epidemic control.

[0091] At this point, the various embodiments of the present invention have been introduced. According to the above description, those skilled in the art should have a clear understanding of the present invention.

[0092] For certain implementations, if they are not the key content of the present invention and are well known to ordinary technicians in the relevant technical field, they are not described in detail in the drawings or text of the specification due to space limitations. In this case, reference can be made to relevant existing technologies for understanding.

[0093] The present invention can be implemented by means of hardware including several different elements and by means of a suitably programmed computer. The various component embodiments of the present invention can be implemented in hardware, or in software modules running on one or more processors, or in a combination thereof. The physical implementation of the hardware structure includes but is not limited to physical devices, which include but are not limited to transistors, memristors, DNA computers, single-chip microcomputers, microprocessors or digital signal processors (DSPs). In addition, the present invention is not directed to any specific programming language. It should be understood that the content of the present invention can be implemented using various programming languages, and the description of specific languages ​​herein is intended to disclose the best implementation of the present invention.

[0094] Those skilled in the art should understand that in the claims and description of the present invention, the word "comprising" does not exclude the existence of elements (or steps) not listed in the claims. The word "a" or "an" preceding an element (or step) does not exclude the existence of multiple such elements (or steps).

[0095] Furthermore, the above embodiments are provided merely to enable the present invention to satisfy legal requirements, and the present invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

[0096] Similarly, it should be understood that in order to simplify the present invention, in the above description of the exemplary embodiments of the present invention, the various features of the present invention are sometimes grouped together into a single embodiment, figure, or description thereof. However, the method of the invention should not be interpreted as reflecting the following intention: the claimed invention requires more features than the features explicitly stated in each claim. More specifically, as reflected in the claims, each inventive aspect lies in less than all the features of the previous single embodiment. Moreover, the embodiments can be mixed and matched with each other or with other embodiments based on design and reliability considerations, that is, the technical features in different embodiments can be freely combined to form more embodiments. Therefore, the claims following the specific embodiment are hereby explicitly incorporated into the specific embodiment, wherein each claim itself serves as a separate embodiment of the present invention.

[0097] The above specific embodiments provide a detailed description of the objectives, technical means and beneficial effects of the present invention. It should be understood that the purpose of the detailed description is to enable those skilled in the art to understand the present invention more clearly, and it is not used to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial colony culture intelligent image acquisition system, characterized in that: include: A loading platform, used for carrying a culture container containing an observation sample; An image acquisition device, whose lens is aimed at the observation sample on the first side of the object loading platform; A transmission light source, arranged on a second side of the object-carrying platform away from the image acquisition device; An annular light source, comprising: an annular base, arranged between the observation sample and the image acquisition device, the inner diameter of which is larger than the outer diameter of the culture container; and reflecting light units and ultraviolet light units arranged at intervals on the annular base, the number of both being more than 3, wherein a plurality of reflecting light units constitute a reflecting light source, and a plurality of ultraviolet light units constitute an ultraviolet light source; A control device, whose signal output end is connected to the transmission light source, light valve, reflection light source, ultraviolet light source and image acquisition device, is used to control the opening and closing of the transmission light source, reflection light source and ultraviolet light source respectively, and control the image acquisition device to acquire images of microbial colonies.

2. The microbial colony culture intelligent image acquisition system according to claim 1, characterized in that: Also includes: A light valve is disposed between the observation sample and the transmission light source, and can controllably transmit or block the light emitted from the transmission light source to the observation sample; The control device has a signal output end connected to the light valve and is used to control the opening and closing of the light valve.

3. The microbial colony culture intelligent image acquisition system according to claim 2, characterized in that: The control device comprises: The first storage unit is used to store N image acquisition modes for collecting microbial colony images, where N≥2, and each image acquisition mode includes: a light source mode; The main control unit is used to execute the following control logic: Step D1, reading the image acquisition mode in the first storage unit; Step D2, according to the light source mode in the image acquisition mode, controlling the opening and closing of the transmission light source, the light valve, the reflection light source, and the ultraviolet light source; Step D3, controlling the image acquisition device to acquire images of the microbial colonies; Step D4, storing the microbial colony image in a second storage unit.

4. The microbial colony culture intelligent image acquisition system according to claim 3, characterized in that: The light valve is a liquid crystal light valve; And / or, the culture container is: a plate; And / or, the culture container is inverted, the transmission light source and the light valve are arranged above the culture container, and the annular light source and the image acquisition device are arranged below the culture container; And / or, the transmission light source is a flat light source, the light valve is a liquid crystal light valve, and the horizontal extension range of the liquid crystal light valve is greater than the horizontal extension range of the flat light source; And / or, in the annular light source, both the reflective light unit and the ultraviolet light unit are LED units; and the number of both the reflective light unit and the ultraviolet light unit is greater than 10; And / or, in the annular light source, the reflected light unit is a white light LED unit, and the reflected light source is a white light source; And / or, the control device is designed based on a single chip microcomputer; And / or, further comprising: a temperature sensor and / or a humidity sensor, the sensing contact of which extends into the chamber where the culture dish is located; And / or, the main control unit is further used to adopt M different image acquisition modes in the same shooting cycle to alternately execute steps D1 to D4, 2≤M≤N; And / or, the main control unit is further used to use different image acquisition modes to acquire images of the microbial colony for the T1th capturing cycle and the T2th capturing cycle among the T capturing cycles, T≥10, T1≠T2; And / or, the first storage unit and the main control unit are inseparably integrated on the same board, and the second storage unit is detachably installed on the board; Or, the first storage unit and the second storage unit are different partitions of the same memory; And / or, in the control logic executed by the main control unit, in step D4, one or more of the following auxiliary information are integrated with the microbial colony image as traceable data with an original watermark for storage: culture medium and / or microorganism, image acquisition mode, temperature and / or humidity, operator, image acquisition time.

5. The microbial colony culture intelligent image acquisition system according to claim 3, characterized in that: The first storage unit is further used to store a mapping relationship database, wherein the optional items of the mapping relationship in the mapping relationship database are one or more of the following: culture medium, microorganism, observation purpose, and the dependent variable is: image acquisition mode; The main control unit is also used to execute the following control logic: Step A, receiving one or more optional items of microbial colony culture; Step B, searching the image acquisition mode corresponding to the received optional item in the mapping relationship database, and recommending the image acquisition mode to the user; Step C: receiving the user's selection of the image acquisition mode.

6. The microbial colony culture intelligent image acquisition system according to claim 5, characterized in that: In the control logic executed by the main control unit: (1) The recommended image acquisition mode is one Step C includes: receiving a user's determination of the image acquisition mode; or, receiving a user's modification of the image acquisition mode; or, receiving a user's rejection of the image acquisition mode, and providing the user with a choice of a custom image acquisition mode; (2) The recommended image acquisition modes are multiple Step B also includes: prompting the user with information characteristics of the microbial colony image that can be obtained by each image acquisition mode; Step C includes: receiving a user's selection of a certain image acquisition mode among multiple image acquisition modes; or receiving a user's selection of alternating shooting of two or more image acquisition modes among the multiple image acquisition modes.

7. The microbial colony culture intelligent image acquisition system according to claim 5, characterized in that: In the image acquisition mode, the light source mode is selected from the following light source modes: ① Transmission light source mode: the transmission light source is turned on, the light valve is opened, and the light emitted by the transmission light source is emitted to the observed sample through the light valve, presenting a bright background of the observed sample; the reflection light source and ultraviolet light source are turned off; ② Reflection light source dark background mode: the transmission light source is turned on, the light valve is closed, and a dark background of the observed sample is presented; the ultraviolet light source is turned off; and the reflection light source is turned on; ③ Reflection light source bright background mode: the transmission light source is turned on, the light valve is opened, and a bright background of the observed sample is presented; The ultraviolet light source is turned off; the reflective light source is turned on; ④ Integrated light source mode, the transmission light source is turned on, the light valve is closed; the reflection light source is turned on; the ultraviolet light source is turned off; ⑤ The first ultraviolet light mode, the transmission light source is turned off, the light valve is closed, and a black background of the observed sample is presented; the reflection light source is turned off, and the ultraviolet light source is turned on; ⑥ The second ultraviolet light mode, the transmission light source is turned off, the light valve is closed, and a black background of the observed sample is presented; the reflection light source and the ultraviolet light source are turned on.

8. The microbial colony culture intelligent image acquisition system according to claim 7, characterized in that: The image acquisition mode also includes: a shooting mode, and the shooting mode further includes one or more of the following: a camera mode, a white balance setting, an acquisition frequency, and an acquisition duration; In the control logic executed by the main control unit, the step D2 further includes: setting parameters of the image acquisition unit according to the shooting mode in the image acquisition mode.

9. The microbial colony culture intelligent image acquisition system according to claim 8, characterized in that: (1) The optional items are: observation purpose, and the mapping relationship includes at least one of the following; 1.1 Observation purpose: Colony surface morphology Its corresponding dependent variable is the image acquisition mode using the following light source modes: reflected light source dark background mode or reflected light source bright background mode; 1.2 Observation purpose: the density of the group The corresponding dependent variable is the image acquisition mode using the following light source modes: transmission light source mode; (2) The optional items are: microorganisms, and the mapping relationship includes at least one of the following; 2.1 The microorganism is Escherichia coli Its corresponding dependent variable is the image acquisition mode using the following shooting mode: the acquisition frequency is 30 minutes per image, and the acquisition duration is 24 hours; 2.2 Microorganism is beta-hemolytic streptococcus Its corresponding dependent variable is the image acquisition mode using the following shooting mode: acquisition frequency is 30 minutes per image, and acquisition duration is 48 hours; 2.3 The microorganism is Staphylococcus aureus; The corresponding dependent variable is the image acquisition mode using the following shooting mode: the acquisition frequency is 30 minutes per image, and the acquisition duration is 48 hours; 2.4 The microorganism is Aspergillus niger; The corresponding dependent variable is the image acquisition mode using the following shooting mode: continuously acquiring images with transmitted light source and reflected light source, with an interval of 5 seconds, two pictures as a group, an acquisition frequency of 2 hours per group, and an acquisition time of 120 hours.

10. The microbial colony culture intelligent image acquisition system according to claim 8, characterized in that: The optional items in the mapping relationship are: microorganisms and culture media; (1) The culture medium is EMB and the microorganism is Escherichia coli; The image acquisition modes corresponding to the dependent variables include: reflected light source dark background mode, camera mode between 8-12, red light white balance between 35-45, blue light white balance between 15-20; the acquisition frequency is 30 minutes per image, and the acquisition time is 24 hours; (2) The culture medium is Columbia blood agar plate and the microorganism is beta-hemolytic Streptococcus. The image acquisition modes corresponding to the dependent variable include the following two: ① Transmitted light source mode, camera mode is between 8-10, red light white balance is between 35-45, blue light white balance is between 15-20; ② In the reflected light source dark background mode, the camera mode is between 10-12, the red light white balance is between 35-45, and the blue light white balance is between 15-20; ③Integrated light source mode, camera mode is between 8-10, red light white balance is between 35-45, blue light white balance is between 15-20; In the above three image acquisition modes, the acquisition frequency is 30 minutes per image and the acquisition time is 48 hours.

11. The microbial colony culture intelligent image acquisition system according to claim 8, characterized in that: The optional items in the mapping relationship are: culture medium, microorganism, and observation purpose; (1) The culture medium is BP, and the microorganism is Staphylococcus aureus. 1.1 Observation purpose: transparent ring and / or precipitation ring The image acquisition modes corresponding to the dependent variables of the above self-selected items are: transmitted light source mode, camera mode between 8-10, red light white balance between 35-45, and blue light white balance between 15-20; 1.2 Observation purpose: Black heart characteristics; The image acquisition modes corresponding to the dependent variables of the above self-selected items are: reflected light source bright background mode, camera mode between 8-10, red light white balance between 35-45, and blue light white balance between 15-20; The above two types of images form a group, with a collection frequency of 30 minutes per group and a collection time of 48 hours; (2) The culture medium is Red Bengal plate and the microorganism is Aspergillus niger; 2.1 Observation purpose: colony counting The image acquisition modes corresponding to the dependent variables of the above self-selected items are: ① Transmitted light source mode, camera mode is between 8-10, red light white balance is between 35-45, and blue light white balance is between 15-20; 2.2 Observation objectives: colony size, color, and mycelium distribution There are two image acquisition modes for the above options corresponding to the dependent variable: ① In the reflected light source dark background mode, the camera mode is between 10-12, the red light white balance is between 35-45, and the blue light white balance is between 15-20; ②Integrated light source mode, camera mode is between 8-12, red light white balance is between 35-45, blue light white balance is between 15-20; 2.3 Observation purpose: Fluorescence phenomenon The image acquisition modes corresponding to the dependent variables of the above self-selected items are: ④ UV light source mode, camera mode is between 13-15, red light white balance is between 35-45, and blue light white balance is between 15-20; The above four images form a group, with a collection frequency of 2 hours per group and a collection time of 120 hours.

12. A microbial expert-assisted analysis system, characterized in that: include: The microbial colony culture intelligent image acquisition system according to any one of claims 3 to 11, wherein the main control unit is further used to alternately perform steps D1 to D4 in M ​​different image acquisition modes in the same shooting cycle, M≥2; An intelligent analysis system, which uses the microbial colony culture intelligent image acquisition system to perform combined analysis on the microbial colony image sequences obtained through different image acquisition modes, and annotates the microbial colony images; The types of microorganisms in the observed samples are unknown; the intelligent analysis system infers possible target microorganisms by performing combined analysis on microbial colony image sequences.

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