Viscous fertilized egg debonding agent based on magnesium-calcium ion chelation

Abstract

The invention discloses a viscous fertilized egg debonding agent based on magnesium-calcium ion chelation, which is an aqueous solution of a magnesium-calcium ion chelating agent, and can be chelated with magnesium-calcium metal ions to form a stable compound only by putting viscous fertilized eggs into the debonding agent to be soaked for 8-12 minutes and keeping slight stirring. Therefore, the combination of magnesium-calcium metal ions and proteins or polysaccharides on the surfaces of the fertilized eggs is destroyed, the adhesiveness of the surfaces of the fertilized eggs is effectively weakened, and the air permeability of the fertilized eggs is prevented from being influenced by adhesion and clustering of the fertilized eggs; meanwhile, in the hatching process, impurity attachment can be reduced, and the phenomena of microorganism breeding and disease transmission caused by impurity attachment are avoided. Besides, mechanical damage to the fertilized eggs caused by slurry, talcum powder or intense stirring can be effectively reduced, the structural integrity of the fertilized eggs can be effectively protected, and the adverse influence on the transparency of a water body and the dissolved oxygen level due to the fact that particles are suspended in water can also be overcome.

Description

Technical Field

[0001] The invention belongs to the field of aquaculture, and in particular relates to a sticky fertilized egg debonding agent based on magnesium and calcium ion chelation. Background Art

[0002] In aquatic animal farming, the rate of artificial reproduction directly affects the aquaculture output. However, since the surface of the eggs of some aquatic animals is highly sticky, the fertilized eggs formed are sticky fertilized eggs, such as the great taki six-line fish ( Hexagrammos otakii ), directly affecting the artificial reproduction rate of aquatic animals. One of the reasons is that sticky fertilized eggs are very likely to cause egg masses to stick together, affecting the ventilation of the fertilized eggs; another reason is that sticky fertilized eggs are prone to adhere to impurities during hatching, causing microbial growth and disease transmission, which reduces the hatching rate and survival rate of fertilized eggs. For this reason, people have been committed to de-sticking sticky fertilized eggs.

[0003] Existing debonding methods mostly use mud, talcum powder or mechanical stirring. Mud and talcum powder are mainly used to block the stickiness between fertilized eggs through physical adsorption and covering effects, but during the operation, the particles of mud or talcum powder will cause mechanical damage to the fertilized eggs due to improper stirring strength and time control. In addition, mud and talcum powder particles suspended in water will reduce the transparency of the water body and affect the dissolved oxygen level, thereby forming environmental stress on the fertilized eggs. Mechanical stirring is a strong stirring, which can easily cause damage to the surface of the fertilized eggs, affecting the quality and hatching rate of the fertilized eggs.

[0004] Magnesium and calcium ion chelators are substances that can chelate with magnesium and calcium metal ions to form stable complexes, such as sodium tripolyphosphate (STPP), and are often used as food additives and industrial raw materials. So far, there have been no reports on the use of magnesium and calcium ion chelators as debonding agents for sticky fertilized eggs. Summary of the invention

[0005] The present invention aims at solving the above-mentioned technical problems existing in the prior art and provides a viscous fertilized egg debonding agent based on magnesium and calcium ion chelation.

[0006] The technical solution of the present invention is: a viscous fertilized egg debonding agent based on magnesium and calcium ion chelation, characterized in that it is an aqueous solution of magnesium and calcium ion chelating agent.

[0007] The preferred magnesium and calcium ion chelating agent is sodium tripolyphosphate.

[0008] Preferably, the mass concentration of the magnesium-calcium ion chelating agent is 0.5-3%.

[0009] Preferably, the mass concentration of the magnesium-calcium ion chelating agent is 1-2%.

[0010] Preferably, the aqueous solution temperature of the magnesium-calcium ion chelating agent is 14-16° C. and the pH value is 7.5-8.2.

[0011] This invention first confirmed that Mg 2+ and Ca 2+ It is the key factor that causes the egg surface to have strong stickiness, so the aqueous solution of magnesium-calcium ion chelating agent is used as the debonding agent for sticky fertilized eggs. It is only necessary to place the fertilized eggs in the debonding agent and soak them for 8-12 minutes and keep them slightly stirred to chelate with magnesium-calcium metal ions to form a stable complex, thereby destroying the combination of magnesium-calcium metal ions with proteins or polysaccharides on the surface of fertilized eggs, effectively weakening the adhesion of the fertilized egg surface, and avoiding the fertilized eggs from sticking together and affecting the aeration of the fertilized eggs; at the same time, during the hatching process, it can reduce the adhesion of impurities and avoid the phenomenon of microbial growth and disease transmission caused by the adhesion of impurities. In addition, the present invention can effectively reduce the mechanical damage to the fertilized eggs caused by mud, talcum powder or strong stirring, effectively protect the integrity of the fertilized egg structure, and overcome the adverse effects of particles suspended in water on the transparency and dissolved oxygen level of the water body. In short, the present invention can effectively improve the debonding efficiency of sticky fertilized eggs, improve the hatching rate and survival rate of sticky fertilized eggs, and then improve the artificial breeding rate and yield of aquatic animals, which is suitable for large-scale production applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The present invention proves that Mg 2+ and Ca 2+ This is a micrograph of the fish eggs that make the roe of the otaki red snapper sticky.

[0013] Figure 2 It is a schematic diagram of the effect of the embodiment of the present invention on the debonding effect of the sticky fertilized eggs of Hesperus otakii. DETAILED DESCRIPTION Example 1

[0014] The invention discloses a viscous fertilized egg debonding agent based on magnesium and calcium ion chelation, which is an aqueous solution of sodium tripolyphosphate, wherein the mass concentration of the sodium tripolyphosphate is 1%, the temperature of the aqueous solution is 14-16°C, and the pH value is 7.5-8.2.

[0015] Embodiment 2: The invention discloses a viscous fertilized egg debonding agent based on magnesium and calcium ion chelation, which is an aqueous solution of sodium tripolyphosphate, wherein the mass concentration of the sodium tripolyphosphate is 2%, the temperature of the aqueous solution is 14-16°C, and the pH value is 7.5-8.2.

[0016] Embodiment 3: The invention discloses a viscous fertilized egg debonding agent based on magnesium and calcium ion chelation, which is an aqueous solution of sodium tripolyphosphate, wherein the mass concentration of the sodium tripolyphosphate is 3%, the temperature of the aqueous solution is 14-16°C, and the pH value is 7.5-8.2.

[0017] experiment: 1. Metal ion concentration gradient experiment on Otaki hexapod roe The eggs of Otaki hexapod were divided into four treatment groups, namely treatment groups 1-4, which were immersed in different concentrations of NaCl, KCl, CaCl2 and MgCl2 for the same time. The first group was immersed in NaCl solutions with concentrations of 10 mmol / L, 20 mmol / L, 30 mmol / L, and 50 mmol / L; the second group was immersed in KCl solutions with concentrations of 10 mmol / L, 20 mmol / L, 30 mmol / L, and 50 mmol / L; the third group was immersed in CaCl2 solutions with concentrations of 5 mmol / L, 10 mmol / L, 15 mmol / L, and 30 mmol / L; and the fourth group was immersed in MgCl2 solutions with concentrations of 5 mmol / L, 10 mmol / L, 15 mmol / L, and 30 mmol / L. Microscopic observation after immersion treatment showed the following results: Figure 1 shown. Figure 1 (A)-(D) are the microscopic observation results of the first group of fish eggs after being treated with different concentrations of NaCl solution. The results show that the viscosity of the roe of the big fish is not enhanced, indicating that the NaCl concentration gradient + Unactivated fish eggs further produce stickiness; Figure 1 (E)-(H) are the microscopic observation results of the second group of fish eggs after being treated with different concentrations of KCl solution. The results show that the viscosity of the roe of the big fish is not enhanced, indicating that the KCl concentration gradient + Unactivated fish eggs further produce stickiness; Figure 1 (I)-(L) are the microscopic observation results of the third group of fish eggs after being treated with different concentrations of CaCl2 solution. The results show that the viscosity of the roe of the big fish is enhanced, indicating that the roe is sensitive to CaCl2. 2+ The stimulation is very sensitive, and the concentration gradient of Ca + It can activate fish eggs to further produce stickiness; Figure 1 (M)-(P) are the microscopic observation results of the fourth group of fish eggs after being treated with different concentrations of MgCl2 solution. The results show that the viscosity of the roe of the big fish is enhanced, indicating that the roe is sensitive to MgCl2. 2+ The stimulation is very sensitive, and the concentration gradient of Mg 2+ It can activate fish eggs to further produce stickiness; The experimental results confirmed that Mg 2+ and Ca 2+ It is the key metal ion that makes the eggs of the otaki six-line fish sticky.

[0018] 2. Experimental study on debonding of fertilized eggs of Hexapod ocellaris using the debonding agent of Example 1 of the present invention Take the debonding agent prepared in Example 1, collect the fertilized eggs of the big head fish after passing through the CaCl2 and MgCl2 solutions in the above experiment 1, and then soak them in the debonding agent at a concentration of 200-300 g / L for 8 minutes, and keep stirring slightly to promote the separation of sticky substances on the surface of the eggs; the temperature during the immersion process is controlled at 14-16°C, the pH value is maintained at 7.5-8.2, and the physical and chemical conditions are consistent with the incubation process to ensure the vitality and quality of the fertilized eggs; after the immersion treatment, gently rinse the fertilized eggs 3 times with physiological saline or deionized water to remove residual sodium tripolyphosphate solution.

[0019] The fertilized eggs of Hexapoda otakii after the above debonding treatment were observed under a microscope. The debonding effect was as follows: Figure 2 shown. Figure 2 The results show that the surface of the fertilized eggs of the big head fish soaked in the debonding agent of Example 1 of the present invention is non-sticky, and the integrity rate of the fertilized eggs reaches more than 98%, which can effectively improve the debonding efficiency of the fertilized eggs and the subsequent hatching rate and survival rate of the fertilized eggs.

Claims

1. A viscous fertilized egg debonding agent based on magnesium and calcium ion chelation, characterized in that It is an aqueous solution of magnesium and calcium ion chelating agent.

2. The viscous fertilized egg debonding agent based on magnesium and calcium ion chelation according to claim 1, characterized in that The magnesium and calcium ion chelating agent is sodium tripolyphosphate.

3. The viscous fertilized egg debonding agent based on magnesium and calcium ion chelation according to claim 1, characterized in that The mass concentration of the magnesium-calcium ion chelating agent is 0.5-3%.

4. The viscous fertilized egg debonding agent based on magnesium and calcium ion chelation according to claim 3, characterized in that The mass concentration of the magnesium-calcium ion chelating agent is 1-2%.

5. The viscous fertilized egg debonding agent based on magnesium and calcium ion chelation according to claim 4, characterized in that The temperature of the aqueous solution of the magnesium-calcium ion chelating agent is 14-16° C., and the pH value is 7.5-8.2.

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