An anti-aging composition containing a protein and a small molecule plant fermentation broth and its application

Abstract

The present invention discloses an anti-aging composition containing protein and small molecule plant fermentation broth and its application, relating to the field of biotechnology. The present invention provides an anti-aging composition, which is prepared from the following raw materials: kudzu root, polygonatum sibiricum, ampelopsis grossedentata, alpinia oxyphylla, clove, ginseng, american ginseng, water, and compound lactobacillus. The anti-aging composition prepared by the present invention can effectively scavenge free radicals, has strong antioxidant effects, and has an obvious improvement effect on skin aging. Moreover, the components are mild and the applicable range is relatively wide.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and particularly to an anti-aging composition containing protein and small molecule plant fermentation broth and its application. Background Art

[0002] Aging is an inevitable process during growth, an inevitable law in the life process, and an inflammatory state, which is characterized by a gradual decline in antioxidant function, followed by an increase in oxidative damage caused by reactive oxygen species and other oxidation products, manifested as structural degradation and functional decline. There are currently various theories about aging, including the free radical theory, the telomere theory, the mitochondrial damage theory, the immunosenescence theory, the calorie restriction theory, etc. The free radical theory states that aging is caused by excessive reactive oxygen species in the body, and age-related loss of function is due to the accumulation of reactive oxygen species, resulting in oxidative damage and tissue damage. The ability to scavenge free radicals is one of the important methods for evaluating antioxidant activity and anti-aging cosmetics. Currently, the ability to scavenge free radicals is mainly measured by clearing indicators such as DPPH free radicals, superoxide anion free radicals, and hydroxyl free radicals, so as to evaluate its antioxidant performance.

[0003] Active substances with antioxidant and anti-wrinkle effects are crucial for anti-skin aging. Skin aging is the most intuitive manifestation of body aging. Skin aging is divided into endogenous aging and exogenous aging. Compared with endogenous aging, exogenous aging has a greater impact on the skin. Exogenous aging often causes damage to the molecular structure of skin cells and related functional changes, thus accelerating skin aging.

[0004] Chinese Patent CN114042024A discloses a whitening and anti-wrinkle hand cream containing plant extracts and its preparation method. Motherwort, peony flower, and safflower are rich in total flavonoids. Through multi-step extraction, the total flavonoid components in motherwort, peony flower, and safflower are fully extracted, supplemented with deionized water, vitamin C, and vitamin E, to prepare a whitening and anti-wrinkle hand cream containing plant extracts, which has excellent free radical scavenging performance, thus inhibiting the generation of wrinkles. Moreover, vitamin C can inhibit the generation of peroxidized lipids and the activity of tyrosinase, thus inhibiting the synthesis of melanin. Vitamin E has a strong antioxidant effect, can delay cell aging, and has natural ingredients and no irritation to the skin.

[0005] Chinese Patent CN107714555A discloses a pure plant moisturizing and anti-wrinkle eye cream and its preparation method, which includes the following raw materials in parts by weight: 20-30 parts of orchid, 22-30 parts of schisandra chinensis, 20-30 parts of chamomile, 20-30 parts of eclipta prostrata, 17-25 parts of kudzu root, 17-25 parts of notoginseng, 14-22 parts of liquorice, 20-30 parts of polygonum multiflorum, 17-25 parts of cactus, 25-35 parts of rhodiola rosea, 3-6 parts of sweet almond oil, and an appropriate amount of water. This invention is prepared from pure plant raw materials. Through reasonable compatibility, the components act synergistically to promote qi and activate blood circulation, clear heat and reduce swelling, and tonify the liver and kidney. It can promote blood circulation in the eye area skin, enhance cell vitality, repair damaged cells and provide them with rich nutrients, deeply moisturize the eye area skin, improve problems such as eye dryness, fade fine lines, and have a good anti-wrinkle effect.

[0006] However, in the current existing technologies, there are few studies on the preparation of compositions by co-fermenting probiotics and plants. It is very necessary to provide products with good antioxidant and anti-wrinkle effects. Summary of the Invention

[0007] The purpose of the present invention is to provide an anti-aging composition containing protein and small molecule plant fermentation broth and its application.

[0008] To achieve the above-mentioned invention purpose, the technical solution of the present invention is as follows:

[0009] On the one hand, the present invention provides an anti-aging composition, which is prepared from the following raw materials: kudzu root, polygonatum sibiricum, ampelopsis grossedentata, alpinia oxyphylla, clove, ginseng, american ginseng, water, and compound lactobacillus.

[0010] Specifically, the anti-aging composition is prepared from the following raw materials in parts by weight: 3.5-7 parts of kudzu root, 1.5-3.0 parts of polygonatum sibiricum, 1.0-2.0 parts of ampelopsis grossedentata, 1.0-2.0 parts of alpinia oxyphylla, 0.4-1 part of clove, 2-6 parts of ginseng, 3-8 parts of american ginseng, 80-85 parts of water, and 2-8 parts of compound lactobacillus.

[0011] Further, the anti-aging composition is prepared from the following raw materials in parts by weight: 5 parts of kudzu root, 2 parts of polygonatum sibiricum, 1.5 parts of ampelopsis grossedentata, 1.5 parts of alpinia oxyphylla, 0.4 part of clove, 2 parts of ginseng, 3 parts of american ginseng, 80 parts of water, and 5 parts of compound lactobacillus.

[0012] Specifically, the preparation of the anti-aging composition includes: mixing kudzu root, polygonatum sibiricum, ampelopsis grossedentata, alpinia oxyphylla, clove, ginseng, american ginseng and water, adjusting the pH to 2-7, inoculating compound lactobacillus, fermenting, concentrating to a relative density of 0.9-1.2 at 20 °C, centrifuging, and sterilizing.

[0013] Specifically, the inoculation amount of the compound lactobacillus is 1-5%;

[0014] Further, the inoculation amount of the compound lactobacillus is 4%.

[0015] Further, the fermentation conditions are fermentation at 35-39°C for 5-8 days.

[0016] Further, the centrifugation conditions are centrifugation at 3000-5000 r / min for 10-30 min.

[0017] Specifically, the compound lactobacillus consists of Lactobacillus mucosae fermentum and Lactobacillus acidophilus.

[0018] Further, the mass ratio of Lactobacillus mucosae fermentum to Lactobacillus acidophilus is 1:(1-3);

[0019] Still further, the mass ratio of Lactobacillus mucosae fermentum to Lactobacillus acidophilus is 1:2.5.

[0020] On the other hand, the present invention provides a product containing the above anti-aging composition, and the product further contains one or more of mussel protein, collagen, and fibronectin.

[0021] Specifically, the product further includes 0.1-0.5 parts by weight of mussel protein, 0.005-0.1 parts by weight of collagen, and 0.0002-0.003 parts by weight of fibronectin.

[0022] Further, the product further includes 0.3 parts by weight of mussel protein, 0.02 parts by weight of collagen, and 0.002 parts by weight of fibronectin.

[0023] Specifically, the product includes pharmaceuticals, cosmetics, and hygiene products.

[0024] Further, the product is a cosmetic.

[0025] Specifically, the dosage form of the product includes, but is not limited to, gastrointestinal dosage forms and non-gastrointestinal dosage forms according to the administration method.

[0026] Further, the gastrointestinal dosage forms include, but are not limited to, tablets, powders, granules, solutions, capsules, emulsions, suspensions, and oils.

[0027] Further, the non-gastrointestinal dosage forms include, but are not limited to: injection dosage forms, respiratory dosage forms, skin dosage forms, mucosal dosage forms, and cavity dosage forms.

[0028] Furthermore, the injectable dosage forms include, but are not limited to, intravenous injections, intramuscular injections, subcutaneous injections, intradermal injections, and intracavitary injections.

[0029] Furthermore, the respiratory dosage forms include, but are not limited to, sprays, aerosols, and powder aerosols.

[0030] Furthermore, the skin dosage forms include, but are not limited to, lotions, ointments, topical solutions, hard plasters, pastes, and patches.

[0031] Furthermore, the mucosal dosage forms include, but are not limited to, eye drops, nasal drops, ophthalmic ointments, sublingual tablets, and film dressings.

[0032] Furthermore, the cavity dosage forms include, but are not limited to, suppositories, aerosols, effervescent tablets, drops, and dripping pills.

[0033] In another aspect, the present invention provides a method for preparing the above-mentioned product, comprising the following steps:

[0034] (1) Add excipients to water and mix well to obtain a first mixture;

[0035] (2) Add the above-mentioned anti-aging composition to the first mixture, mix, and then add mussel protein, collagen, and fibronectin to obtain the product.

[0036] Specifically, the product is a cosmetic.

[0037] Specifically, the cosmetics include, but are not limited to, essence, lotion, cream, facial mask, eye cream, neck cream, hand cream, facial cleanser, liquid foundation, and body lotion.

[0038] Specifically, the cosmetic is essence.

[0039] Specifically, the water in step (1) is deionized water sterilized at high temperature.

[0040] In another aspect, the present invention provides the application of the above-mentioned anti-aging composition or the above-mentioned product in the preparation of an antioxidant product.

[0041] Specifically, the antioxidant effect is to scavenge free radicals.

[0042] Furthermore, the free radicals include ABTS free radicals, DPPH free radicals, and hydroxyl free radicals.

[0043] Furthermore, the free radical is a DPPH free radical.

[0044] Specifically, the products include drugs, cosmetics, and hygiene products.

[0045] Further, the product is a cosmetic.

[0046] Specifically, the cosmetics include, but are not limited to, essence, lotion, cream, facial mask, eye cream, neck cream, hand cream, and body lotion.

[0047] On the other hand, the present invention provides the use of the above anti-aging composition or the above product in the preparation of anti-aging products.

[0048] Specifically, the products include pharmaceuticals, cosmetics, and hygiene products.

[0049] Further, the product is a cosmetic.

[0050] Specifically, the cosmetics include, but are not limited to, essence, lotion, cream, facial mask, eye cream, neck cream, hand cream, and body lotion.

[0051] Specifically, the product further includes excipients.

[0052] Further, the excipients include one or more of butylene glycol, glycerin, polyethylene glycol, 1,2 - hexanediol, 1,2 - pentanediol, octyldecanol, and ethylhexylglycerin.

[0053] Still further, the excipients are butylene glycol and 1,2 - hexanediol.

[0054] Specifically, the product includes 3 - 8 parts by weight of butylene glycol and 2 - 4 parts by weight of 1,2 - hexanediol.

[0055] Further, the product includes 3 parts by weight of butylene glycol and 2 parts by weight of 1,2 - hexanediol.

[0056] According to some embodiments of the present invention, the present invention provides a cosmetic, and the cosmetic includes the above anti-aging composition.

[0057] Specifically, the cosmetics include, but are not limited to, essence, lotion, cream, facial mask, eye cream, neck cream, hand cream, and body lotion.

[0058] Specifically, the cosmetic may further include matrix raw materials and conventional excipients.

[0059] Further, the matrix raw materials include, but are not limited to, gum raw materials, powder raw materials, oil raw materials, wax raw materials, coagulants, and surfactants.

[0060] Still further, the conventional excipients include, but are not limited to, antioxidants, binders, lubricants, moisturizers, preservatives, whitening agents, thickeners, emulsifiers, and cosmetic nutritional additives.

[0061] Furthermore, the cosmetic also includes adjuvants.

[0062] Further, the adjuvants include one or more of butylene glycol, glycerin, polyethylene glycol, 1,2 - hexanediol, 1,2 - pentanediol, octyldecanol, and ethylhexylglycerin.

[0063] Still further, the adjuvants are butylene glycol and 1,2 - hexanediol.

[0064] Specifically, the cosmetic includes 3 - 8 parts by weight of butylene glycol and 2 - 4 parts by weight of 1,2 - hexanediol.

[0065] Further, the cosmetic includes 3 parts by weight of butylene glycol and 2 parts by weight of 1,2 - hexanediol.

[0066] The beneficial effects of the present invention are as follows:

[0067] The anti - aging composition prepared by the present invention can effectively scavenge free radicals, has strong antioxidant effects, and can promote skin repair, improve skin wrinkles and skin texture, significantly improve skin aging, and has mild components and a wide range of applications. Detailed Embodiments

[0068] In order to make the technical means, creative features, achieved purposes, and functions of the present invention easy to understand, the following specific embodiments are used to further clarify the present invention. However, the following embodiments are only the preferred embodiments of the present invention, not all of them. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative efforts all belong to the protection scope of the present invention. In the following embodiments, unless otherwise specified, the operation methods used are conventional operation methods, the equipment used is conventional equipment, and the equipment materials used in each embodiment are the same.

[0069] Sources of experimental materials:

[0070] Lactobacillus plantarum NDC75017 and Lactobacillus acidophilus NCFM, preserved strains in the Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, with viable cell counts of 2.0×10 10 CFU / g; Lactobacillus casei subsp. casei NCU011056, a preserved strain in the State Key Laboratory of Food Science and Technology, Nanchang University, with viable cell counts of 2.0×10 10 CFU / g;

[0071] Lactobacillus mucosae fermentum is from the China Center of Industrial Culture Collection CICC 25124, with viable cell counts of 2.0×10 10 CFU / g;

[0072] Lactobacillus acidophilus is derived from NCFM, the Key Laboratory of Dairy Science of Northeast Agricultural University, with a viable count of 2.0×10 10 CFU / g;

[0073] Mussel protein: purchased from Guangdong Xuanjia Medical & Health Technology Co., Ltd.;

[0074] Collagen: purchased from Zhuhai Jibakang Biotechnology Co., Ltd.;

[0075] Fibronectin: purchased from Zhengzhou Fuberui Biotechnology Co., Ltd.;

[0076] Butanediol: commercially available;

[0077] 1,2 - Hexanediol: commercially available;

[0078] Citric acid: commercially available.

[0079] Example 1

[0080] Preparation of anti - aging composition:

[0081] 1. Strain expansion culture: Under aseptic conditions, pick one loop of Lactobacillus mucosae and Lactobacillus acidophilus cells with an inoculation loop respectively, transfer them into different sterilized test tubes, draw curves, and culture them horizontally in the test tubes at 32°C for 7 days. After the colonies grow, transfer and inoculate again under the same operating conditions as above, and the culture conditions are the same. After 3 generations of activation of the strains, they are used for experiments.

[0082] 2. Fermentation: Add 3.5 parts of Pueraria lobata, 1.5 parts of Polygonatum sibiricum, 1.0 part of Ampelopsis grossedentata, 1.0 part of Alpinia oxyphylla, 0.4 part of Eugenia caryophyllata, 2 parts of Panax ginseng, 3 parts of Panax quinquefolius, and 82.5 parts of water into an Erlenmeyer flask, adjust the pH to 3.5 with citric acid, mix well, add a cotton plug to the bottle mouth, wrap it with newspaper, and sterilize it in a portable autoclave at 115°C for 20 min. Inoculate 2 parts of the activated compound Lactobacillus (the mass ratio of Lactobacillus mucosae and Lactobacillus acidophilus is 1:1) according to an inoculation amount of 4%, and culture it on a shaker at a rotation speed of 120 r / min and a culture temperature of 35°C for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20°C, centrifuge it at 4000 r / min for 20 min, and sterilize it to obtain the anti - aging composition.

[0083] Preparation of essence:

[0084] (1) Add 4 parts of butanediol to 100 parts of water, stir at 90°C for 20 min, cool to 40°C, and add 2 parts of 1,2 - hexanediol and stir evenly to obtain the first mixture;

[0085] (2) Add the anti-aging composition prepared above to the first mixture, mix, then add 0.1 part of mussel protein, 0.01 part of collagen, and 0.0002 part of fibronectin, and mix well to obtain the essence.

[0086] Example 2

[0087] Preparation of anti-aging composition:

[0088] 1. Strain expansion culture: Under sterile conditions, use an inoculation loop to pick up one loop of Lactobacillus mucosae fermentum and Lactobacillus acidophilus cells respectively, transfer them into different sterilized test tubes, draw curves, and culture them horizontally in the test tubes at 32 °C for 7 days. After the colonies grow, transfer them again under the same operating conditions as above, and the culture conditions are the same as above. After 3 generations of activation of the strains, they are used for experiments.

[0089] 2. Fermentation: Add 5 parts of Pueraria lobata, 2 parts of Polygonatum sibiricum, 1.5 parts of Ampelopsis grossedentata, 1.5 parts of Alpinia oxyphylla, 0.4 part of Eugenia caryophyllata, 2 parts of Panax ginseng, 3 parts of Panax quinquefolius, and 80 parts of water into an Erlenmeyer flask, adjust the pH to 3.5 with citric acid, mix well, add a cotton plug to the bottle mouth, wrap it with newspaper, and sterilize it in a portable autoclave at 115 °C for 20 min. Inoculate 5 parts of activated compound Lactobacillus (the mass ratio of Lactobacillus mucosae fermentum and Lactobacillus acidophilus is 1:2.5) according to an inoculation amount of 4%, and culture it on a shaker at a rotation speed of 120 r / min and a culture temperature of 35 °C for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20 °C, centrifuge it at 4000 r / min for 20 min, and sterilize it to obtain the anti-aging composition.

[0090] Preparation of essence:

[0091] (1) Add 3 parts of butanediol to 100 parts of water, stir at 90 °C for 30 min, cool to 40 °C, and add 2 parts of 1,2-hexanediol and stir evenly to obtain the first mixture;

[0092] (2) Add the anti-aging composition prepared above to the first mixture, mix, then add 0.3 part of mussel protein, 0.005 part of collagen, and 0.002 part of fibronectin, and mix well to obtain the essence.

[0093] Example 3

[0094] Preparation of anti-aging composition:

[0095] 1. Strain expansion culture: Under sterile conditions, use an inoculation loop to pick up one loop of Lactobacillus mucosae fermentum and Lactobacillus acidophilus cells respectively, transfer them into different sterilized test tubes, draw curves, and culture them horizontally in the test tubes at 32 °C for 7 days. After the colonies grow, transfer them again under the same operating conditions as above, and the culture conditions are the same as above. After 3 generations of activation of the strains, they are used for experiments.

[0096] 2. Fermentation: Add 7 parts of Pueraria lobata, 3.0 parts of Polygonatum sibiricum, 2.0 parts of Ampelopsis grossedentata, 2.0 parts of Alpinia oxyphylla, 1 part of clove extract, 6 parts of ginseng, 8 parts of American ginseng, and 85 parts of water into an Erlenmeyer flask. Adjust the pH to 3.5 with citric acid, mix well, cover the bottle mouth with a cotton plug, wrap it with newspaper, and sterilize it in a portable autoclave at 115 °C for 20 min. Inoculate 8 parts of activated compound Lactobacillus (the mass ratio of Lactobacillus mucosae fermentum and Lactobacillus acidophilus is 1:3) according to an inoculation amount of 4%, and culture it on a shaker at a rotation speed of 120 r / min and a culture temperature of 35 °C for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20 °C, centrifuge it at 4000 r / min for 20 min, and sterilize it to obtain the anti-aging composition.

[0097] Preparation of essence:

[0098] (1) Add 8 parts of butanediol to 100 parts of water, stir at 90 °C for 30 min, cool to 40 °C, and add 4 parts of 1,2-hexanediol and stir evenly to obtain the first mixture;

[0099] (2) Add the anti-aging composition prepared above to the first mixture, mix, and then add 0.5 part of mussel protein, 0.1 part of collagen, and 0.003 part of fibronectin, and mix evenly to obtain the essence.

[0100] Comparative Example 1

[0101] Preparation of plant extract composition:

[0102] 1. The strain expansion culture is the same as in Example 2.

[0103] 2. Fermentation: Add 8 parts of Pueraria lobata, 4 parts of Polygonatum sibiricum, 2.5 parts of Ampelopsis grossedentata, 2.5 parts of Alpinia oxyphylla, 1.3 parts of cloves, 2 parts of ginseng, 3 parts of American ginseng, and 80 parts of water into an Erlenmeyer flask. Adjust the pH to 3.5 with citric acid, mix well, cover the bottle mouth with a cotton plug, wrap it with newspaper, and sterilize it in a portable autoclave at 115 °C for 20 min. Inoculate compound Lactobacillus (the mass ratio of Lactobacillus mucosae fermentum and Lactobacillus acidophilus is 1:2.5) according to an inoculation amount of 4%, and culture it on a shaker at a rotation speed of 120 r / min and a culture temperature of 35 °C for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20 °C, centrifuge it at 4000 r / min for 20 min, and sterilize it to obtain the anti-aging composition.

[0104] The preparation of the essence is the same as in Example 2.

[0105] Comparative Example 2

[0106] 1. The strain expansion culture is the same as in Example 2.

[0107] 2. Fermentation: Add 5 parts of Pueraria lobata, 2 parts of Polygonatum sibiricum, 1.5 parts of Ampelopsis grossedentata, 1.5 parts of Alpinia oxyphylla, 0.4 part of Eugenia caryophyllata, 5 parts of Panax ginseng, and 80 parts of water into an Erlenmeyer flask. Adjust the pH to 3.5 with citric acid, mix well, cover the bottle mouth with a cotton plug, wrap it with newspaper, and sterilize it in a portable autoclave at 115 °C for 20 min. Inoculate with compound Lactobacillus (the mass ratio of Lactobacillus fermentum and Lactobacillus acidophilus is 1:2.5) according to an inoculation amount of 4%, culture it on a shaker at a rotation speed of 120 r / min, a culture temperature of 35 °C, and ferment for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20 °C, centrifuge at 4000 r / min for 20 min, and sterilize to obtain the anti-aging composition.

[0108] The preparation of the essence is the same as that in Example 2.

[0109] Comparative Example 3

[0110] 1. The strain amplification culture is the same as that in Example 2.

[0111] 2. Fermentation: Add 5 parts of Pueraria lobata, 2 parts of Polygonatum sibiricum, 1.5 parts of Ampelopsis grossedentata, 1.5 parts of Alpinia oxyphylla, 0.4 part of Eugenia caryophyllata, 2 parts of Panax ginseng, 3 parts of American ginseng, and 80 parts of water into an Erlenmeyer flask. Adjust the pH to 3.5 with citric acid, mix well, cover the bottle mouth with a cotton plug, wrap it with newspaper, and sterilize it in a portable autoclave at 115 °C for 20 min. Inoculate with compound Lactobacillus (the mass ratio of Lactobacillus fermentum and Lactobacillus acidophilus is 1:5) according to an inoculation amount of 4%, culture it on a shaker at a rotation speed of 120 r / min, a culture temperature of 35 °C, and ferment for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20 °C, centrifuge at 4000 r / min for 20 min, and sterilize to obtain the anti-aging composition.

[0112] The preparation of the essence is the same as that in Example 2.

[0113] Comparative Example 4

[0114] 1. Strain amplification culture: Under sterile conditions, use an inoculation loop to pick up one loop of the cells of Lactobacillus plantarum, Lactobacillus casei, and Lactobacillus acidophilus respectively, transfer them into different sterilized test tubes, draw curves, and culture them horizontally in the test tubes at 32 °C for 7 days. After the colonies grow, transfer and inoculate them again under the same operating conditions as above, and the culture conditions are the same as above. After the strain goes through 3 generations of activation process, it is used for the experiment.

[0115] 2. Fermentation: Add 5 parts of Pueraria lobata, 2 parts of Polygonatum sibiricum, 1.5 parts of Ampelopsis grossedentata, 1.5 parts of Alpinia oxyphylla, 0.4 parts of Eugenia caryophyllata, 2 parts of Panax ginseng, 3 parts of American ginseng, and 80 parts of water into an Erlenmeyer flask. Adjust the pH to 3.5 with citric acid, mix well, add a cotton plug to the bottle mouth, wrap it with newspaper, and sterilize it in a portable autoclave at 115°C for 20 minutes. Inoculate with a compound Lactobacillus (the mass ratio of Lactobacillus plantarum, Lactobacillus casei, and Lactobacillus acidophilus is 1:0.5:2) according to an inoculation amount of 4%, and culture it on a shaker at a rotation speed of 120 revolutions per minute and a culture temperature of 35°C for 7 days. Concentrate the fermentation broth to a relative density of 1.05 at 20°C, centrifuge it at 4000 r / min for 20 minutes, and sterilize it to obtain the anti-aging composition.

[0116] The preparation of the essence is the same as that in Example 2.

[0117] Experimental Example 1 Detection of Antioxidant Capacity

[0118] Detection method for DPPH free radical scavenging ability:

[0119] (1) Take 4 mL of 2×10 -4 mol / L DPPH solution (weigh DPPH and make up the volume to 250 mL with absolute ethanol to prepare 2×10 -4 mol / L DPPH solution) and 1 mL of 95% ethanol, mix well and react. Use 95% ethanol as the reference, and measure the absorbance value A0 at 515 nm.

[0120] (2) Take 4 mL of 95% ethanol and 1 mL of the sample solution (anti-aging composition) prepared in Examples 1-3 and Comparative Examples 1-4, mix well and react. Use 95% ethanol as the reference, and measure the absorbance value Ax at 515 nm.

[0121] (3) Take 4 mL of 2×10 -4 mol / L DPPH solution and 1 mL of the sample solution prepared in Examples 1-3 and Comparative Examples 1-4, mix well and react. Use 95% ethanol as the reference, and measure the absorbance value Ay at 515 nm.

[0122] Calculate the DPPH free radical scavenging rate according to the following formula:

[0123] DPPH free radical scavenging rate (%) = [1 - (Ay - Ax) / A0] × 100%.

[0124] The test results are shown in the following table:

[0125] Table 1 DPPH free radical scavenging rate

[0126]

[0127] As can be seen from the above table, the scavenging rate of the sample solutions prepared in Examples 1-3 of the present invention for DPPH free radicals is 90-94%. Among them, the antioxidant effect of Example 2 is the best. In Comparative Example 1, the components in the culture medium are outside the protection scope of the present invention, and its antioxidant effect significantly decreases. In Comparative Example 2, American ginseng is replaced with ginseng. In Comparative Example 3, the mass ratio of Lactobacillus mucosae fermentum and Lactobacillus acidophilus is adjusted outside the protection scope of the present application. In Comparative Example 4, changing the composition of the strain reduces the antioxidant effect of the prepared anti-aging composition.

[0128] Experimental Example 2 Detection of anti-wrinkle ability

[0129] The essence prepared in Examples 1-3 and Comparative Examples 1-4 was used as a sample, and the test subjects were 70 healthy female volunteers aged 35-45 with similar facial wrinkle degrees. They were randomly divided into 7 groups, with 10 people in each group, and each group tried one sample. The sample was applied to the wrinkle area of the subjects, the same area was smeared every day, 2 mL was smeared each time, and it was smeared once in the morning and once in the evening for 6 consecutive weeks. The Antera 3D skin analyzer was used to test the reduction degree of the wrinkle area in the wrinkle area of the subjects, and the average value of each group was taken. The test results are shown in the following table:

[0130] Table 2 Degree of reduction in wrinkle area

[0131]

[0132] As can be seen from the above table, the essence prepared in Examples 1-3 of the present invention can effectively reduce the surface wrinkle area after 6 weeks of use. When the dosage of the components exceeds the defined scope of the present application or the components are adjusted (Comparative Examples 1-4), its anti-wrinkle effect significantly decreases.

[0133] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An anti-aging composition, characterized in that, The anti-aging composition is prepared from the following raw materials in parts by weight: 3.5 - 7 parts of kudzu root, 1.5 - 3.0 parts of polygonatum sibiricum, 1.0 - 2.0 parts of ampelopsis grossedentata, 1.0 - 2.0 parts of alpinia oxyphylla, 0.4 - 1 part of clove, 2 - 6 parts of ginseng, 3 - 8 parts of american ginseng, 80 - 85 parts of water, and 2 - 8 parts of compound lactobacillus; The compound lactobacillus consists of lactobacillus mucosae fermentum and lactobacillus acidophilus. The lactobacillus mucosae fermentum is lactobacillus mucosae fermentum CICC 25124, and the lactobacillus acidophilus is lactobacillus acidophilus NCFM; The mass ratio of the lactobacillus mucosae fermentum to the lactobacillus acidophilus is 1:(1 - 3); The preparation of the anti-aging composition includes: mixing kudzu root, polygonatum sibiricum, ampelopsis grossedentata, alpinia oxyphylla, clove, ginseng, american ginseng and water, adjusting the pH to 2 - 7, inoculating compound lactobacillus, fermenting, concentrating to a relative density of 0.9 - 1.2 at 20°C, centrifuging, and sterilizing.

2. The anti-aging composition according to claim 1, wherein The mass ratio of the lactobacillus mucosae fermentum to the lactobacillus acidophilus is 1:2.

5.

3. A product comprising the anti-aging composition according to any one of claims 1-2, characterized in that, The product also includes one or more of mussel protein, collagen, and fibronectin.

4. The product according to claim 3, characterized in that, By weight, it also includes 0.1 - 0.5 part of mussel protein, 0.005 - 0.1 part of collagen, and 0.0002 - 0.003 part of fibronectin.

5. The preparation method of the product according to any one of claims 3-4, characterized in that, It includes the following steps: (1) Adding auxiliary materials to water and mixing evenly to obtain a first mixture; (2) Adding the anti-aging composition according to any one of claims 1 - 2 to the first mixture, mixing, and then adding mussel protein, collagen, and fibronectin to obtain the product.

6. Use of the anti-aging composition according to any one of claims 1 - 2 or the product according to any one of claims 3 - 4 in the preparation of an antioxidant product.

7. The application according to claim 6, wherein The antioxidant product also includes auxiliary materials.

8. The application according to claim 7, characterized in that, The auxiliary materials are selected from one or more of butylene glycol, glycerol, polyethylene glycol, 1,2 - hexanediol, 1,2 - pentanediol, caprylyl glycol, and ethylhexylglycerin.

9. The application according to claim 7, characterized in that The antioxidant product includes 3 - 8 parts of butylene glycol and 2 - 4 parts of 1,2 - hexanediol by weight.

10. Use of the anti-aging composition according to any one of claims 1 - 2 or the product according to any one of claims 3 - 4 in the preparation of an anti-aging product.

11. The application according to claim 10, wherein The anti-aging product also includes auxiliary materials.

12. The application according to claim 11, wherein The auxiliary materials are selected from one or more of butylene glycol, glycerol, polyethylene glycol, 1,2 - hexanediol, 1,2 - pentanediol, caprylyl glycol, and ethylhexylglycerin.

13. The application according to claim 11, wherein The anti-aging product includes 3 - 8 parts of butylene glycol and 2 - 4 parts of 1,2 - hexanediol by weight.

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