Skin external composition containing thiotaurine and saccharomycetes / rice fermentation product filtrate and application of skin external composition

Abstract

The invention provides a skin external composition containing thiotaurine and saccharomycetes / rice fermentation product filtrate and application of the skin external composition, and relates to the technical field of cosmetics. The topical composition for skin comprises thiotaurine and a yeast / rice fermentation product filtrate in a physiologically acceptable medium. According to the application, the thiotaurine and the yeast / rice fermentation product filtrate are combined, so that a synergistic interaction effect can be generated when oxygen free radicals are removed, and the oxidation resistance of the product can be further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin external composition containing thiotaurine and yeast / rice fermentation product filtrate and a use thereof. Background Art

[0002] Skin is a tissue with high metabolic activity. Frequent exposure of the skin to the external environment, including UV and air pollution, can induce the production of harmful reactive oxygen species (ROS), which can cause oxidative damage to various biomacromolecules, including cell membranes, lipids, proteins, RNA and DNA, destroy important cells and increase DNA damage and mutations. When a large amount of oxidants exceeds the capacity of the antioxidant defense system, a condition called oxidative stress occurs. Oxidative stress is caused by an imbalance between oxidants and antioxidants. If ROS are produced excessively or there are insufficient antioxidants in the body or the capacity of the antioxidant cell defense system is reduced, important processes such as intracellular replication, transcription and translation will be damaged, leading to cell death, gene mutation, various pathological processes and increased aging. Oxidative stress is considered to be a contributing factor to the aging process and the pathogenesis of various skin diseases. Antioxidants can terminate the oxidation chain transmission by binding to free radicals and donating their electrons to them, making it impossible for free radicals to attack intracellular biomacromolecules.

[0003] Sebum in the skin has a moisturizing effect and is a very important substance. Squalene in sebum has strong antioxidant, permeability and lubricity, and participates in the antioxidant defense system on the skin surface. However, when it plays an antioxidant role, it turns into squalene monohydroperoxide, which damages the skin's barrier function and makes the skin dry.

[0004] From the perspective of population research, studies have shown that the epidermal peroxidation reaction produces the lowest level of squalene monohydroperoxide in people around 20 years old, and it tends to increase gradually with age. The conversion rate of squalene in the epidermis of patients with atopic dermatitis is higher than that of normal people. Therefore, the epidermal antioxidant capacity of patients with atopic dermatitis is lower than that of healthy people, suggesting that the state of the skin affects the skin's antioxidant defense system.

[0005] Oxidation of human skin does not only occur in the epidermis, but also chain reactions and inflammation caused by environmental factors (ultraviolet rays, smog, etc.) can also cause peroxidation reactions inside the skin. Although the skin has a higher antioxidant system than the epidermis, skin abnormalities and aging can reduce the antioxidant system inside the skin.

[0006] Therefore, there is still a demand to develop antioxidant defense systems that can effectively enhance or supplement the skin. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a skin external composition, which contains thiotaurine and yeast / rice fermentation product filtrate (INCI: SACCHAROMYCES / RICE FERMENT FILTRATE) in a physiologically acceptable medium, wherein the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(8-200).

[0008] Optionally, the mass ratio of thiotaurine to the filtrate of the yeast / rice fermentation product is 1:(10-100). For example, the mass ratio of thiotaurine to the filtrate of the yeast / rice fermentation product can be 1:8, 1:9, 1:10, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, 1:100, 1:110, 1:120, 1:130, 1:140, 1:150, 1:160, 1:170, 1:180, 1:190, 1:200 or any value and range therebetween.

[0009] The amounts of thiotaurine and yeast / rice ferment filtrate to be used in accordance with the present invention may vary within wide limits to determine a suitable amount, depending on the desired cosmetic effect.

[0010] Optionally, the mass concentration of thiotaurine is 0.01%-5%. For example, the mass concentration of thiotaurine can be 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or any value and range therebetween.

[0011] Optionally, the mass concentration of the yeast / rice fermentation product filtrate is 0.01%-95%. For example, the mass concentration of the yeast / rice fermentation product filtrate can be 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or any value and scope therebetween.

[0012] The yeast / rice fermentation product filtrate of the present application refers to the fermentation liquid obtained by fermenting rice with yeast.

[0013] In a specific embodiment, the yeast / rice fermentation product filtrate refers to the fermentation filtrate obtained by removing impurities and sterilizing the fermentation liquid obtained by fermenting rice with yeast.

[0014] The yeast / rice fermentation product filtrate can be prepared according to the method disclosed in the prior art, for example, according to the methods disclosed in patents CN108324633B, CN112618464A, and CN114703074A, but these methods are not the only ones that can prepare brown rice fermentation filtrate.

[0015] The yeast / rice fermentation product filtrate can also be obtained through commercial channels, for example, it can be purchased from Bloomage Biotechnology Co., Ltd.

[0016] The present invention provides a use of the above-mentioned skin external composition in preparing a product for scavenging oxygen free radicals.

[0017] The present invention provides a use of the above-mentioned skin external composition in preparing a product for inhibiting lipid peroxidation.

[0018] The present invention provides a use of the above-mentioned skin external composition in preparing an antioxidant product.

[0019] Furthermore, the composition is used for one or more of repairing the skin barrier, improving skin dryness, and reducing skin sensitivity.

[0020] For example, the product for removing oxygen free radicals, inhibiting lipid peroxidation or anti-oxidation can be an essence, a facial mask, a gel, an emulsion, a foundation or a cream. Those skilled in the art can add corresponding excipients or auxiliary materials according to the type of product.

[0021] Further, solvents (for example, water, oils, hexylene glycol, butylene glycol, 1,3-propylene glycol, etc.) may be added, moisturizers (for example, 1,2-pentanediol, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sodium hyaluronate, trehalose, Tremella fuciformis fruiting body extract, ethylhexylglycerin, sodium γ-polyglutamate, glycerol, butylene glycol, avocado oil, squalane, betaine, etc.) may be added, and thickeners (for example, glycerol, (acrylates / (C10-30) alkyl acrylate) cross-linked polymers, cellulose derivatives, xanthan gum, guar gum, starch and its derivatives, alginate and its derivatives, agar, sodium polyacrylate, carboxyvinyl polymers, bentonite, etc.) may be added. The excipients and adjuvants in the present invention are not limited thereto, and those skilled in the art may add corresponding excipients or adjuvants according to the dosage form and area of ​​use.

[0022] The present invention provides a use of a combination of thiotaurine and yeast / rice fermentation product filtrate applied to the skin surface for non-therapeutic purposes to scavenge oxygen free radicals.

[0023] The present invention provides a use of a combination of thiotaurine and yeast / rice fermentation product filtrate applied to the skin surface for non-therapeutic purposes to inhibit lipid peroxidation.

[0024] In the present invention, there is provided a use of a combination of thiotaurine and yeast / rice fermentation product filtrate applied to the skin surface for non-therapeutic purposes to produce an antioxidant effect.

[0025] Further, the combination of thiotaurine and yeast / rice fermentation product filtrate is used for one or more of repairing skin barrier, improving skin dryness, and reducing skin sensitivity.

[0026] Furthermore, in the combination of thiotaurine and yeast / rice fermentation product filtrate, the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(8-200).

[0027] In some specific embodiments, in the combination of thiotaurine and yeast / rice fermentation product filtrate, the mass ratio of thiotaurine to yeast / rice fermentation product filtrate can be 1:(10-100).

[0028] More specifically, it may be 1:8, 1:9, 1:10, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, 1:100, 1:110, 1:120, 1:130, 1:140, 1:150, 1:160, 1:170, 1:180, 1:190, 1:200 or any value or range therebetween.

[0029] Compared with the prior art, the present invention achieves at least one of the following beneficial effects:

[0030] The present application combines thiotaurine and yeast / rice fermentation product filtrate, which can produce a synergistic effect when scavenging oxygen free radicals, and can further improve the antioxidant performance of the product.

[0031] Based on the above antioxidant effects, the combination of thiotaurine and yeast / rice fermentation product filtrate can not only inhibit the peroxidation of epidermal lipids from an exogenous source, but also enhance the skin's endogenous antioxidant effect. This has a positive effect on repairing the skin barrier, improving skin dryness, and reducing skin sensitivity.

[0032] Furthermore, the yeast / rice fermentation product filtrate also has multiple functions such as improving the product's directional oil control, endogenous hydration and moisturizing, soothing and stabilizing, and anti-glycation damage. Thiotaurine also has the function of regulating biological activity. The combination of the two can fully protect the skin from damage caused by external stimuli and maintain the skin in good condition. DETAILED DESCRIPTION

[0033] In order to more clearly illustrate the overall concept of the application, the following is described in detail by way of embodiment. In the following description, a large amount of specific details are provided to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present invention, some technical features well known in the art are not described.

[0034] If no specific conditions are specified in the examples, the experiments were carried out according to conventional conditions or conditions recommended by the manufacturer.

[0035] Example

[0036] The experiment was tested using an ORAC Assay kit (ab233473) purchased from Abcam. Thiotaurine was purchased from Shanghai Huiwen Biotechnology Co., Ltd., and brown rice fermentation filtrate (INCI: yeast / rice fermentation product filtrate) was purchased from Bloomage Biotech Co., Ltd.

[0037] Introduction to the mechanism of ORAC test method

[0038] ORAC is the abbreviation of Oxygen Radical Absorbance Capacity. The ORAC method is one of the most commonly used methods to detect the ability of antioxidants to resist ROS. The principle is that a free radical initiator such as an azo initiator compound 2,2-azobis(2,-methylpropylimidazole) dihydrochloride is mixed with a fluorescent probe (fluorescein sodium) to destroy the fluorescent probe, causing a change in fluorescence intensity, and the fluorescence decay is quantified by using a fluorometer. The free radical initiator produces peroxy radicals. In the absence of an inhibitor, these free radicals will quickly destroy the fluorescence of the fluorescein dye. By tracking the time course of fluorescence decay and comparing the addition and non-addition of the test substance, the free radical capture ability of the test substance can be estimated. Trolox, a water-soluble analog of vitamin E, is used as a quantitative standard, and a fluorescence analyzer is used for analysis to determine the degree of protection of the compound compared with the standard control antioxidant Trolox. The results are expressed in μMol equivalents of Trolox. Equipment that can automate the measurement and calculation capabilities is commercially available, such as the Fluoroskan FL Microplate Fluorescence and Chemiluminescence Analyzer from Thermo Scientific.

[0039] References for ORAC determination include: Cao G, Alessio H, Cutler R (1993). "Oxygen-radical absorbance capacity assay for antioxidants". Free Radic Biol Med 14(3): 303-11; Ou B, Hampsch-Woodill M, Prior R (2001). "Comparing antioxidant values ​​with the ORAC method". Alternat. Complement. Ther. 2004, 10, 167-160; Cronin, JR.

[0040] Sample preparation

[0041] According to Table 1, thiotaurine and brown rice fermentation filtrate sample solutions with different ratios were prepared using phosphate buffer. The specific preparation method is as follows:

[0042] Comparative Example 1, Comparative Example 2

[0043] Thiotaurine was dissolved in phosphate buffer to obtain 0.25% and 0.5% thiotaurine solutions, respectively.

[0044] Comparative Example 3-Comparative Example 7

[0045] The yeast / rice fermentation product filtrate was dissolved in a phosphate buffer to obtain yeast / rice fermentation product filtrate solutions with mass concentrations of 0.05%, 0.5%, 5%, 10% and 50%, respectively.

[0046] Comparative Example 8

[0047] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.5% thiotaurine and 0.05% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:0.1.

[0048] Comparative Example 9

[0049] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.5% thiotaurine and 0.5% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:1.

[0050] Comparative Example 10

[0051] 3-o-ethylascorbic acid was dissolved in phosphate buffer to obtain a 0.5% 3-o-ethylascorbic acid solution.

[0052] Example 1

[0053] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.5% thiotaurine and 50% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:100.

[0054] Example 2

[0055] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.5% thiotaurine and 10% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:20.

[0056] Example 3

[0057] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.5% thiotaurine and 5% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:10.

[0058] Example 4

[0059] Thiotaurine, yeast / rice fermentation product filtrate, and 3-o-ethyl ascorbic acid are dissolved in phosphate buffer to obtain a composition containing 0.5% thiotaurine, 5% yeast / rice fermentation product filtrate, and 0.5% 3-o-ethyl ascorbic acid, wherein the mass ratio of thiotaurine, yeast / rice fermentation product filtrate, and 3-o-ethyl ascorbic acid is 1:10:1.

[0060] Example 5

[0061] Dissolve thiotaurine and yeast / rice fermentation product filtrate in phosphate buffer to obtain a composition containing 0.25% thiotaurine and 50% yeast / rice fermentation product filtrate, and the mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:200.

[0062] The formulas in Table 1 were tested for antioxidant activity using the ORAC test method.

[0063] ORAC test method

[0064] Add 25 μL of sample or 25 μL of diluted standard (Trolox) control group to a 96-well plate. Then add 150 μL of fluorescein solution to each well, mix well and incubate in a 37°C incubator for 30 minutes. Add 25 μL of free radical initiator solution, mix well and immediately place in a microplate fluorescence and chemiluminescence analyzer to read at an excitation wavelength of 485 nm and an emission wavelength of 538 nm, with a measurement temperature of 37°C, a measurement time of 60 min, and a reading interval of every 1 min.

[0065] Graphpad was used to calculate the area under the fluorescence decay curve (AUC value) of each sample (fluorescence reading of the sample group). The linear formula was fitted with the net AUC value of the standard (Trolox) (AUC value of the standard group - AUC value of the blank group) and the concentration of the standard, and a standard curve was drawn with Trolox as the standard. According to the Trolox line, the net AUC value of the sample (AUC value of the sample group - AUC value of the Blank group) was converted into the corresponding Trolox value and the linear formula was fitted with the sample concentration. The calculation result (ORAC value) was expressed in Trolox equivalent (μmol / g), that is, the oxygen free radical absorption capacity per gram of sample was equivalent to the number of micromoles of Trolox.

[0066] Taking comparative example 2 as an example to simulate the calculation process, the linear formula of the net AUC value of the standard product and the concentration of the standard product is: Net AUC Trolox =1.941C Trolox +5.1776. According to the curve fitted by the standard product, the concentration of Trolox corresponding to the net AUC value of the sample, i.e., C Trolox =(Net AUC 样品 -5.1776) / 1.941 means that the antioxidant capacity of the sample at this concentration is equivalent to the antioxidant capacity of the Trolox concentration. The net AUC values ​​of the samples were converted to the corresponding Trolox concentrations, and a linear formula was fitted with the sample concentration to obtain C Trolox =5680.8C 样品 +0.3333, that is, the ORAC of 0.5% thiotaurine solution = 5680.8*0.5% + 0.3333 = 28.74 umol / g.

[0067] Method for determining synergistic antioxidant activity

[0068] Synergy is determined by comparing the actual measured ORAC value of the composition with the expected or cumulative value of the individual compounds. The predicted ORAC is the combined (cumulative) antioxidant capacity of the various antioxidant compounds in the combination, where each antioxidant compound is measured separately and assuming that they each work independently, the predicted ORAC value of a certain combination can be calculated using the following equation, ORAC (total) = ORAC1 + ORAC2 + ... ORACn (n is the number of actives). When the actual measured ORAC value is significantly greater than the predicted value, there is synergistic antioxidant activity.

[0069] The antioxidant activity of each component alone and in combination was evaluated and the results are shown in Table 1. Assuming that the antioxidant effect is additive, predictions were made for each composition to obtain the predicted ORAC value of the formula and compared with the actual measured ORAC value of the combination of the present invention in Table 1.

[0070] Table 1

[0071]

[0072]

[0073] As can be seen from the results in Table 1, the actual measured ORAC value of the composition of the present invention is significantly greater than the predicted value, indicating that the antioxidant activity of the composition of the present invention is greater than the sum (cumulative value) of the individual antioxidant activities of each component. The composition of the present application shows synergistic antioxidant activity, has a more excellent antioxidant effect, and has a stronger protective effect against the damage of free radicals and reactive oxygen species.

[0074] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A composition for external use on skin, characterized in that: It comprises thiotaurine and yeast / rice fermentation product filtrate in a physiologically acceptable medium, The mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(8-200).

2. The composition for external use on skin according to claim 1, characterized in that The mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(10-100).

3. Use of the skin external composition according to claim 1 or 2 in the preparation of a product for scavenging oxygen free radicals.

4. Use of the composition for external use on skin according to claim 1 or 2 in the preparation of a product for inhibiting lipid peroxidation.

5. Use of the composition for external use on skin according to claim 1 or 2 in the preparation of an antioxidant product.

6. Use of a combination of thiotaurine and yeast / rice fermentation product filtrate for non-therapeutic application to the surface of the skin to scavenge oxygen free radicals.

7. Use of a combination of thiotaurine and yeast / rice fermentation product filtrate for non-therapeutic application to the skin surface to inhibit lipid peroxidation.

8. Use of a combination of thiotaurine and yeast / rice fermentation product filtrate for non-therapeutic application to the surface of the skin to produce an antioxidant effect.

9. The use according to any one of claims 6 to 8, characterized in that in, The mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(8-200).

10. The use according to claim 9, characterized in that in, The mass ratio of thiotaurine to yeast / rice fermentation product filtrate is 1:(10-100).

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