A Heinz body incubation system and incubation method

Abstract

The present invention relates to a Heinz body incubation system and incubation method, including a test tube rack storage area, a test tube moving mechanism, a sample transfer mechanism, a sample incubation mechanism, a sample adding mechanism, a waste disposal device and a washing liquid bucket, wherein the test tube rack storage area is arranged on the left side of the incubation box, the test tube rack storage area is provided with a plurality of test tube rack placement positions, the test tube moving mechanism is slidably arranged at the rear end of the test tube rack storage area, the sample transfer mechanism is arranged at the front end of the test tube rack storage area, the sample incubation mechanism and the sample adding mechanism are arranged on the right side of the incubation box, the sample adding mechanism is arranged on the sample incubation mechanism, and the waste disposal device and the washing liquid bucket are arranged below the test tube rack storage area, the test tube moving mechanism, the sample transfer mechanism, the sample incubation mechanism and the sample adding mechanism. Through this mechanism, the problems of complicated steps, long time consumption, high labor cost, difficult uniformity in experimental operation, and difficult guarantee of experimental quality in the manual experiment process are solved.

Description

Technical Field

[0001] The present invention relates to the field of medical experimental technology, and in particular to a Heinz body incubation system and incubation method. Background Art

[0002] Heinz bodies are denatured globin bodies attached to red blood cells. Unstable hemoglobin is easily oxidized, denatured, and precipitated to form insoluble Heinz bodies. In particular, when the patient's red blood cells are incubated in vitro with acetylphenylhydrazine peroxidase reagent, a large number of Heinz bodies are generated in the red blood cells. When stained with dyes, Heinz bodies appear in the shape of fine particles, coarse particles, or small beads. They are often located at the edge of red blood cells, vary in size, and are stained bright purple. They can be used as one of the indicators for identifying unstable hemoglobin.

[0003] The current procedure for Heinz bodies is to add 180 μl (3-4 drops) of reaction solution to a glass test tube, then add 25 μl (1 drop) of blood. Mix the mixture in a 37.0°C water bath for 3-5 hours, then add a small drop of 2% crystal violet and mix thoroughly. After 10 minutes, remove a drop of the specimen, place it on a glass slide, cover it with a glass slide, and observe under an oil immersion lens. Automated methods and equipment are currently unavailable for this assay, requiring the entire process to be performed manually. This process is complex, time-consuming, and labor-intensive, making it difficult to standardize experimental procedures and ensure quality. Summary of the Invention

[0004] The present invention provides a Heinz body incubation system and incubation method, which solves the problems of cumbersome steps, long time consumption, high labor cost, difficulty in uniformity of experimental operation, and difficulty in ensuring the quality of the experiment during manual experiment.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A Heinz body incubation system includes a test tube rack storage area, a test tube moving mechanism, a sample transfer mechanism, a sample incubation mechanism, a sample adding mechanism, a waste disposal device and a washing liquid bucket. The incubation system is arranged in an incubation box, the test tube rack storage area is arranged on the left side of the incubation box, the test tube rack storage area is provided with multiple test tube rack placement positions, the test tube moving mechanism is slidably arranged at the rear end of the test tube rack storage area, the sample transfer mechanism is arranged at the front end of the test tube rack storage area, the sample incubation mechanism and the sample adding mechanism are arranged on the right side of the incubation box, the sample adding mechanism is arranged on the sample incubation mechanism, and the waste disposal device and the washing liquid bucket are arranged below the test tube rack storage area, the test tube moving mechanism, the sample transfer mechanism, the sample incubation mechanism and the sample adding mechanism.

[0007] The beneficial effect of adopting the above-mentioned further scheme is that the test tube moving mechanism in the incubation system moves the test tubes in the test tube rack storage area to the sample incubation mechanism, and the sample adding mechanism adds the sample in the sample transfer mechanism to the test tube. At the same time, the sample adding mechanism then adds other reagents to the test tube containing the sample, and incubates it through the sample incubation mechanism. The sample adding mechanism is then cleaned with the washing liquid in the washing liquid bucket, and the waste liquid and the sample test tubes after testing are transferred to a second waste disposal device. Automated instrumentation can achieve precise sample addition. The sample is mixed with the sample reaction liquid with an oxidizing effect in the sample incubation mechanism and an incubation reaction is carried out. The unstable hemoglobin is oxidized, denatured, and precipitated to form insoluble Heinz bodies, which are then stained with a dye to dye the Heinz bodies purple. This improves personnel work efficiency, standardizes experimental operations, and ensures the quality of experimental results.

[0008] Furthermore, the test tube moving mechanism includes a sliding mechanism, a first telescopic rod, a second telescopic rod and a test tube sleeve, the lower end of the first telescopic rod is fixedly arranged on the sliding mechanism, the upper end of the first telescopic rod is fixedly connected to one end of the second telescopic rod, and the other end of the second telescopic rod is fixedly connected to the test tube sleeve.

[0009] The beneficial effect of adopting this further solution is that the test tube moving mechanism, via the first telescopic rod, drives the second telescopic rod to slide left and right on the sliding mechanism. Simultaneously, the first and second telescopic rods move up and down, and forward and backward, moving the test tube holder to the test tube rack storage area and aligning it with the test tube. The test tube moving mechanism then moves the test tube to a designated position in the sample incubation mechanism; the test tube moving mechanism can reciprocate on the sliding mechanism, enabling automated and precise test tube retrieval.

[0010] Furthermore, the test tube sleeve includes a test tube sleeve opening, a test tube clamp, a test tube clamp telescopic rod, a clamping member, a clamping member support rod, a spring, and an induction detection device. The interior of the test tube sleeve is a hollow trapezoid with a smaller upper end and a larger lower end. The lower end is the test tube sleeve opening. The test tube clamp telescopic rod is provided at the top of the test tube clamp. Two clamping member support rods are provided at the bottom of the test tube clamp. A clamping member is provided at the bottom of each of the two clamping member support rods. The two ends of the spring are respectively connected to the clamping member support rods on both sides. The side wall of the test tube sleeve opening is also provided with an induction detection device.

[0011] The sliding mechanism includes a slide rail, a screw, a threaded slider, a mechanism gear and a motor. The two ends of the screw are arranged on both sides of the slide rail. One end of the screw is provided with a mechanism gear, and the mechanism gear is connected to the output end of the motor. The threaded slider is fixed on the slide rail, and the threaded slider is movably set on the screw. The threaded slider is also fixed with a first telescopic rod.

[0012] The beneficial effect of adopting the above further solution is that the motor drives the mechanism gear to rotate, thereby causing the screw to rotate forward or reverse. When the screw rotates forward or reverse, it moves the threaded slider along the length of the slide rail, thereby driving the first and second telescopic rods to move, moving the test tube sleeve over the test tube to be transferred, and tightening the test tube with the test tube sleeve to transfer the test tube.

[0013] Furthermore, the sample transfer mechanism includes a sample feed area, a sample buffer area, a sample adding area, a sample rack limiting moving unit, a sample rack, a conveying structure, a first sensing mechanism and a first driving mechanism. The sample feed area is arranged on the right side of the sample transfer mechanism, and the sample buffer area is arranged on the left side of the sample transfer mechanism. The sample buffer area includes a first sample buffer area and a second sample buffer area. The sample feed area is connected to the sample adding area through the sample adding area entrance, and the first sample buffer area and the second sample buffer area are connected through the sample buffer area entrance. The bottom of the sample feed area, the sample buffer area and the sample adding area are all provided with a conveying structure, and the bottom of the conveying structure A first sensing mechanism and a first driving mechanism are provided, and a first sensing mechanism is provided at the entrance of the sample adding area and the entrance of the sample buffer area. The sample rack is slidably connected with the sample injection area, the sample buffer area and the sample rack limiting moving unit; the sample rack limiting moving unit is composed of a PLC controller, a first sample adding hole and a first test tube hole are provided on the top of the sample injection area, a barcode scanner is provided on the side of the sample injection area and at a position corresponding to the first sample adding hole, the bottom of the first test tube hole is connected to the waste disposal device, a plurality of sample tube sleeves are provided on the top of the sample rack, and a barcode scanning port is provided on the side of the plurality of sample tube sleeves.

[0014] The beneficial effect of adopting this further solution is that the sample rack is placed in the sample introduction area, and the first sensing mechanism and the first driving mechanism transfer the sample rack to the sample addition area. The barcode scanner then transmits the information of the sample to be tested to the incubation system. After the sample to be tested in the sample rack is added to the test tube, the first sensing mechanism and the first driving mechanism transfer the sample rack to the sample buffer area. This allows the sample transfer mechanism to transfer and place the samples to be tested and the samples that have been tested separately, making it easier for staff to distinguish them.

[0015] Furthermore, the first sensing mechanism further includes a first sample rack baffle and a second sample rack baffle, wherein the first sample rack baffle is arranged at the entrance of the sample adding area, and the second sample rack baffle is arranged at the entrance of the sample buffer area;

[0016] A third sample rack card slot is provided on opposite sides of the sample rack, a first sample rack card slot and a second sample rack card slot are symmetrically provided on the bottom of the sample rack, a first sample rack card strip and a second sample rack card strip are provided on the bottom of the sample injection area and the sample buffer area, and a third sample rack card strip is provided on the sides of the sample injection area and the sample buffer area, the first sample rack card slot is engaged and slidably connected to the first sample rack card strip, the second sample rack card slot is engaged and slidably connected to the second sample rack card strip, and the third sample rack card slot is engaged and slidably connected to the third sample rack card strip.

[0017] The beneficial effect of adopting the above further solution is that the first sample rack slot, the second sample rack slot and the third sample rack slot on the sample rack are respectively engaged and slidably connected with the first sample rack clip strip, the second sample rack clip strip and the third sample rack clip strip, thereby forming a stable connection with the sample rack and achieving precise movement of the sample rack during the movement process.

[0018] At the same time, the sample rack touches the first sample rack baffle or the second sample rack baffle during the movement, and the first sensing mechanism senses and determines that the sample rack moves to the sample adding area or the sample buffer area.

[0019] Furthermore, the sample incubation mechanism includes an incubation chamber, an incubation tray, a reagent storage chamber, a sample mixing mechanism, a second sensing mechanism, a second driving mechanism, an incubation chamber cover, a third driving mechanism and a third sensing mechanism. The incubation tray is arranged in the incubation chamber, a through hole is opened in the center of the incubation tray, the reagent storage chamber passes through the through hole and is placed in the through hole, a reagent position is arranged in the reagent storage chamber, a second sensing mechanism and a second driving mechanism are arranged in the incubation chamber, the incubation chamber cover is arranged above the incubation chamber, and a second test tube hole, a first test tube hole and a second driving mechanism are opened on the incubation chamber cover. The reagent storage chamber has two sample loading holes and a third sample loading hole. A reagent storage chamber cover is provided above the reagent storage chamber, and a fourth sample loading hole is provided on the reagent storage chamber cover. The sample mixing mechanism is correspondingly provided below the third sample loading hole, and a third driving mechanism and a third sensing mechanism are provided below the sample mixing mechanism. Sealing strips are provided on the incubation chamber cover and the reagent storage chamber cover, and multiple limiting parts are provided on the incubation chamber cover and the reagent storage chamber cover. Multiple limiting grooves are provided on the side walls of the incubation chamber and the reagent storage chamber, and the multiple limiting parts are adapted to be connected with the multiple limiting grooves.

[0020] The beneficial effect of adopting the above-mentioned further scheme is that a constant temperature is provided for the incubation tray and the reaction sample by the second sensing mechanism provided in the incubation chamber. The test tubes of the samples to be tested in the incubation tray are incubated at a constant temperature. After the sample, reaction liquid and dye in the test tube are evenly mixed by the third driving mechanism, the test tubes of the samples to be tested are placed in the incubation tray for constant temperature incubation, so that the sample, reaction liquid and dye react more fully. The second driving mechanism drives the incubation tray to rotate in the incubation chamber, so that the incubated sample rotates to the specified position. Accurate sample addition of the automated instrument is achieved. The sample is mixed with the sample reaction liquid with an oxidizing effect in the sample incubation mechanism and incubated to react. The unstable hemoglobin is oxidized and denatured to precipitate to form insoluble Heinz bodies, which are then dyed purple with the dye. The work efficiency of the personnel is improved, and the experimental operation is standardized, and the quality of the experimental results is guaranteed.

[0021] Furthermore, the sampling mechanism has a first sampling mechanism and a second sampling mechanism. The sampling mechanism includes a third telescopic rod, a rotating arm, a motor and a sampling needle. The lower end of the third telescopic rod is provided with a gear. The gear at the lower end of the third telescopic rod is connected to the gear of the motor. The upper end of the third telescopic rod is fixedly connected to one end of the rotating arm. The other end of the rotating arm is fixedly connected to the sampling needle. The upper end of the sampling needle is connected to the washing liquid tube and the pressure tube. The other end of the pressure tube is connected to the pressure pump. The other end of the washing liquid tube is connected to the washing liquid bucket and the pressure pump. The ends of the washing liquid tubes are connected to the sensing device.

[0022] The beneficial effect of adopting the above further solution is that the first loading mechanism adds the sample to the test tube to be tested, and the second loading mechanism adds the reaction liquid reagent and dye to the test tube to be tested. After the first and second loading mechanisms complete the sample addition, the first and second loading mechanisms clean the loading needles in the first and second loading mechanisms with the washing liquid in the washing liquid bucket, and discharge the waste liquid into the waste liquid bucket. This ensures that the loading needles are clean before continuing to the next step. This prevents residual reagents in the loading needles from being carried over to other steps and affecting the reaction effects of other reagents.

[0023] Furthermore, the waste disposal device includes a waste storage box and a waste liquid barrel. The bottom of the first test tube hole is connected to the waste storage box, and the bottom of the first sample adding mechanism is connected to the waste liquid barrel and the washing liquid barrel.

[0024] The beneficial effect of adopting the above further solution is that after the first and second sample loading mechanisms have completed loading, the sample loading needles in the first and second sample loading mechanisms are cleaned with the washing liquid in the washing liquid bucket, and the waste liquid is discharged into the waste liquid bucket. This ensures that the sample loading needles are clean before continuing to the next step. This prevents residual reagents in the sample loading needles from being carried over to other steps and affecting the reaction effects of other reagents.

[0025] Furthermore, an incubation box cover is provided on the top of the incubation box, and the incubation box cover is hinged to one side of the incubation box.

[0026] The beneficial effect of adopting the above further solution is that by hingedly connecting the incubation box cover to one side of the incubation box, the incubation box cover can be conveniently placed on the incubation box, thereby ensuring the cleanliness and hygiene of the incubation system.

[0027] On the other hand, a Heinz body incubation method is based on a Heinz body incubation system, and the incubation method is:

[0028] S1: The Heinz body incubation system is automatically reset, and the second sensing mechanism of the sample transfer mechanism controls the temperature to 37°C, the temperature required for sample reaction; the tester empties the waste storage box and waste liquid bucket, places a test tube rack containing clean test tubes in the test tube rack storage area, and fills the wash liquid bucket with wash liquid;

[0029] S2: Place the sample to be tested into the sample tube sleeve of the sample rack, with the barcode of the sample to be tested facing the barcode scanning port, and place the sample rack into the sample inlet area. The sample rack and the sample transfer mechanism are slidably connected by the engagement of the card slot and the card strip. The gravity of the sample rack acts on the conveying structure, and the first sensing mechanism transmits the received signal to the first driving mechanism. The first driving mechanism drives the conveying structure to move the sample rack, and the sample rack limit movement unit controls the sample rack to move from the sample inlet area to the sample addition area.

[0030] S3: Scan the barcode of the sample to be tested with a barcode scanner to transmit the sample information to the incubation system. The incubation system controls the test tube moving mechanism to work, and the test tube moving mechanism transfers the test tube from the test tube rack storage area to the sample incubation mechanism.

[0031] S4: The second sensing mechanism of the sample incubation mechanism senses that the test tube enters the incubation chamber, and the second driving mechanism drives the incubation plate to rotate;

[0032] S5: The first loading mechanism draws the sample from the first loading hole and adds the sample into the test tube through the second loading hole, and then washes the loading needle of the first loading mechanism with a washing solution;

[0033] S6: The second loading mechanism draws the reaction liquid reagent from the fourth loading hole, and adds the reaction liquid reagent into the test tube through the third loading hole, and mixes the sample in the test tube with the reaction liquid reagent and incubates them, and then washes the loading needle of the second loading mechanism with a washing solution;

[0034] S7: After all the samples to be tested are added to the sample rack, the sample rack limiting moving unit moves the sample rack and moves the sample rack to a designated position in the sample buffer area;

[0035] S8: After the sample and the reaction liquid reagent in the test tube are evenly mixed and incubated, the second loading mechanism draws the stain from the fourth loading well and adds the stain into the test tube through the third loading well. At the same time, the sample mixing mechanism mixes the sample to be tested, the reaction liquid reagent, and the stain sample in the test tube and incubates them. Then, the loading needle of the second loading mechanism is cleaned with a wash solution.

[0036] S9: After the sample has been incubated for 10 minutes, the second driving mechanism drives the incubation plate to rotate and rotates the test tube containing the sample to be tested to below the third loading well. The loading needle of the second loading mechanism is positioned above the third loading well, and the sample to be tested is sampled and loaded onto the slide. Then, the loading needle of the second loading mechanism is cleaned with a wash solution.

[0037] S10: After the reagent incubated in the sample test tube to be tested is sampled and tested, the second driving mechanism drives the incubation disk to rotate, and moves the test tube to the second test tube hole. The test tube moving mechanism removes the test tube from the second test tube hole and transfers it to the waste storage box through the first test tube hole;

[0038] S11: Repeat steps S1 to S10 multiple times according to the incubation requirements, and incubate in sequence.

[0039] The beneficial effect of adopting the above-mentioned further solution is that the present invention realizes precise sample addition by automated instruments through the fully automated incubation system. The sample is mixed with the oxidizing sample reaction solution in the sample incubation mechanism and incubated to oxidize and denature the unstable hemoglobin and precipitate it into insoluble Heinz bodies. The Heinz bodies are then stained with a dye to dye them purple. This improves work efficiency, standardizes experimental operations, and ensures the quality of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a schematic structural diagram of a Heinz body incubation system of the present invention;

[0041] Figure 2 This is a schematic structural diagram of an incubation structure of a Heinz body incubation system according to the present invention;

[0042] Figure 3 This is a schematic structural diagram of an incubation tray of a Heinz body incubation system according to the present invention;

[0043] Figure 4 This is a schematic structural diagram of an incubation chamber of a Heinz body incubation system according to the present invention;

[0044] Figure 5 This is a schematic diagram of the structure of an incubation chamber cover of a Heinz body incubation system of the present invention;

[0045] Figure 6This is a schematic structural diagram of a reagent storage chamber cover of a Heinz body incubation system according to the present invention;

[0046] Figure 7 This is a schematic structural diagram of a sample transfer mechanism of a Heinz body incubation system according to the present invention;

[0047] Figure 8 This is a schematic structural diagram of a sample rack for a Heinz body incubation system according to the present invention;

[0048] Figure 9 This is a schematic structural diagram of a sample loading mechanism of a Heinz body incubation system according to the present invention;

[0049] Figure 10 This is a schematic structural diagram of a test tube set of a Heinz body incubation system of the present invention;

[0050] Figure 11 This is a schematic structural diagram of a test tube moving mechanism of a Heinz body incubation system of the present invention;

[0051] Figure 12 The present invention is a flow chart of a method for incubating Heinz bodies.

[0052] In the accompanying drawings, the components represented by the reference numerals are as follows: 1. incubator; 10. test tube rack storage area; 101. test tube rack placement position; 11. incubator cover; 12. test tube moving mechanism; 121. sliding mechanism; 1211. slide rail; 1212. screw; 1213. threaded slider; 1214. mechanism gear; 124. first telescopic rod; 125. second telescopic rod; 126. test tube sleeve; 1261. test tube sleeve opening; 1262. test tube clamp; 1263. test tube clamp telescopic rod; 1264. clamping member; 1265. Clamping support rod; 1266, spring; 1267, induction detection device; 13, sample transfer mechanism; 131, sample loading area; 1311, first sample loading hole; 1312, first test tube hole; 1313, barcode scanner; 1314, first sample rack card strip; 1315, second sample rack card strip; 1316, third sample rack card strip; 132, sample buffer area; 1321, first sample buffer area; 13211, sample loading area entrance; 13213, sample buffer area entrance; 1322, second sample buffer area; 13 3. Sample introduction area; 134. Sample rack limit moving unit; 135. Sample rack; 1351. Sample tube sleeve; 1352. Barcode scanning port; 1353. First sample rack slot; 1354. Second sample rack slot; 1355. Third sample rack slot; 136. Conveying structure; 137. First sensing mechanism; 1371. First sample rack baffle; 1372. Second sample rack baffle; 14. Sample incubation mechanism; 141. Incubation chamber; 1411. Limiting slot; 142. Incubation tray; 143. Reagent storage chamber; 14 31. Reagent position; 1432. Reagent storage chamber cover; 1433. Fourth sample loading hole; 144. Sample mixing mechanism; 147. Incubation chamber cover; 1471. Second test tube hole; 1472. Second sample loading hole; 1473. Third sample loading hole; 1474. Sealing strip; 1475. Limiting piece; 15. Sample loading mechanism; 151. First sample loading mechanism; 152. Second sample loading mechanism; 153. Third telescopic rod; 154. Rotating arm; 155. Sample loading needle; 161. Waste storage box; 162. Waste liquid barrel; 17. Washing liquid barrel. DETAILED DESCRIPTION

[0053] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0054] Example 1

[0055] like Figure 1-11As shown, the incubation system includes a test tube rack storage area 10, a test tube moving mechanism 12, a sample transfer mechanism 13, a sample incubation mechanism 14, a sample adding mechanism 15, a second waste disposal device and a washing liquid bucket 17. The incubation system is arranged in the incubation box 1, the test tube rack storage area 10 is arranged on the left side of the incubation box 1, the test tube rack storage area 10 is provided with a plurality of test tube rack placement positions 101, the test tube moving mechanism 12 is slidably arranged at the rear end of the test tube rack storage area 10, the sample transfer mechanism 13 is arranged at the front end of the test tube rack storage area 10, the sample incubation mechanism 14 and the sample adding mechanism 15 are arranged on the right side of the incubation box 1, the sample adding mechanism 15 is arranged on the sample incubation mechanism 14, and the waste disposal device and the washing liquid bucket 17 are arranged below the test tube rack storage area 10, the test tube moving mechanism 12, the sample transfer mechanism 13, the sample incubation mechanism 14 and the sample adding mechanism 15.

[0056] Specifically, in the incubation system, the test tube moving mechanism 12 moves the test tubes from the test tube rack storage area 10 to the sample incubation mechanism 14. The sample loading mechanism 15 then adds the sample from the sample transfer mechanism 13 to the test tube. Simultaneously, the sample loading mechanism 15 adds other reagents to the test tube containing the sample, which is then incubated by the sample incubation mechanism 14. The sample loading mechanism 15 is then cleaned with the washing liquid in the washing liquid bucket 17, and the waste liquid and the tested sample tubes are transferred to a second waste disposal device.

[0057] like Figure 1-11 As shown, the test tube moving mechanism 12 includes a sliding mechanism 121, a first telescopic rod 124, a second telescopic rod 125, and a test tube sleeve 126. The lower end of the first telescopic rod 124 is fixedly disposed on the sliding mechanism 121, the upper end of the first telescopic rod 124 is fixedly connected to one end of the second telescopic rod 125, and the other end of the second telescopic rod 125 is fixedly connected to the test tube sleeve 126.

[0058] Specifically, the test tube moving mechanism 12 uses a first telescopic rod 124 to drive a second telescopic rod 125 to slide left and right on the sliding mechanism 121. Simultaneously, the first and second telescopic rods 124, 125 move up and down, and forward and backward, moving the test tube holder 126 to the test tube rack storage area 10 and aligning it with the test tube. The test tube moving mechanism 12 then moves the test tube to a designated position in the sample incubation mechanism 14. The test tube moving mechanism 12 reciprocates on the sliding mechanism 121, enabling automated and precise test tube retrieval.

[0059] like Figure 1-11As shown, the test tube sleeve 126 includes a test tube sleeve opening 1261, a test tube clamp 1262, a test tube clamp telescopic rod 1263, a clamping member 1264, a clamping member support rod 1265, a spring 1266 and an induction detection device 1267. The interior of the test tube sleeve 126 is a hollow trapezoid with a smaller upper end and a larger lower end. The lower end is the test tube sleeve opening 1261. The test tube clamp telescopic rod 1263 is set at the top of the test tube clamp 1262. Two clamping member support rods 1265 are set at the bottom of the test tube clamp 1262. A clamping member 1264 is set at the bottom of each of the two clamping member support rods 1265. The two ends of the spring 1266 are respectively connected to the clamping member support rods 1265 on both sides. The side wall of the test tube sleeve opening 1261 is also provided with an induction detection device 1267.

[0060] The sliding mechanism 121 includes a slide rail 1211, a screw 1212, a threaded slider 1213, a mechanism gear 1214 and a motor. The two ends of the screw 1212 are arranged on both sides of the slide rail 1211. One end of the screw 1212 is provided with a mechanism gear 1214, and the mechanism gear 1214 is connected to the output end of the motor. The threaded slider 1213 is fixedly set on the slide rail 1211, and the threaded slider 1213 is movably set on the screw 1212. The threaded slider 1213 is also fixedly provided with a first telescopic rod 124.

[0061] Specifically, in this embodiment, the motor drives the mechanism gear 1214 to rotate, thereby causing the screw 1212 to rotate forward or reverse. When the screw 1212 rotates forward or reverse, it moves the threaded slider 1213 along the length of the slide rail 1211, thereby driving the first telescopic rod 124 to move left and right. The first telescopic rod 124 is then adjusted up and down based on the height of the test tube, and the second telescopic rod 125 is adjusted forward and backward. The test tube sleeve 126 is then moved over the test tube to be transferred, and the test tube is clamped by the test tube sleeve 126 to transfer the test tube.

[0062] In this embodiment, the interior of the test tube holder 126 is a hollow trapezoidal shape, with a smaller upper end and a larger lower end. The lower end forms a test tube opening 1261. A test tube clamp telescopic rod 1263 is located at the top of the test tube clamp 1262, and a clamping support rod 1265 is located at the bottom of the test tube clamp 1262. The ends of a spring 1266 are connected to the clamping support rods 1265 on either side. A sensing device 1267 is also provided on the sidewall of the test tube opening 1261. When the first telescopic rod 124 and the test tube holder 126 move downward, the test tube enters the test tube holder 126 through the test tube opening 1261. The sensing device 1267 senses the test tube and transmits a signal to the system, thereby controlling the first telescopic rod 124 to stop moving downward and simultaneously controlling the upward movement of the test tube clamp telescopic rod 1263.

[0063] In this embodiment, when the test tube clamp telescopic rod 1263 works, it drives the clamping member support rod 1265 to move up and down. When the clamping member support rod 1265 moves upward, it drives the clamping member 1264 to move upward. At this time, since the interior of the test tube sleeve 126 is a hollow trapezoid with a smaller upper end and a larger lower end, the clamping member 1264 is subjected to the pressure of the test tube sleeve 126 during the upward movement. The clamping member 1264 contracts inward to clamp the test tube, and the first telescopic rod 124 and / or the second telescopic rod 125 work to drive the test tube sleeve 126 upward. When the clamping member support rod 1265 moves downward, it drives the clamping member 1264 to move downward. At this time, since the interior of the test tube sleeve 126 is a hollow trapezoid with a smaller upper end and a larger lower end, the pressure exerted by the test tube sleeve 126 on the clamping member 1264 during the downward movement decreases. Under the action of the spring 1266 in the middle of the clamping member support rod 1265, the clamping member 1264 is driven to expand outward, releasing the test tube.

[0064] like Figure 1-11 As shown, the sample transfer mechanism 13 includes a sample injection area 133, a sample buffer area 132, a sample adding area 131, a sample rack limiting moving unit 134, a sample rack 135, a conveying structure 136, a first sensing mechanism 137 and a first driving mechanism. The sample injection area 133 is arranged on the right side of the sample transfer mechanism 13, and the sample buffer area 132 is arranged on the left side of the sample transfer mechanism 13. The sample buffer area 132 includes a first sample buffer area 1321 and a second sample buffer area 1322. The sample injection area 133 is connected to the sample adding area 131 through a sample adding area inlet 13211, and the first sample buffer area 1321 is connected to the second sample buffer area 1322 through a sample buffer area inlet 13213. The bottom of the sample injection area 133, the sample buffer area 132 and the sample adding area 131 are all provided with a conveying structure 136. The conveying structure 137 is provided with a first sensing mechanism 137. 6 is provided with a first sensing mechanism 137 and a first driving mechanism. The first sensing mechanism 137 is provided at the sample loading area entrance 13211 and the sample buffer area entrance 13213. The sample rack 135 is slidably connected with the sample injection area 133, the sample buffer area 132 and the sample rack limiting and moving unit 134; the sample rack limiting and moving unit 134 is composed of a PLC controller. The top of the sample injection area 133 is provided with a first sample loading hole 1311 and a first test tube hole 1312. A barcode scanner 1313 is provided on the side of the sample injection area 133 and at a position corresponding to the first sample loading hole 1311. The bottom of the first test tube hole 1312 is connected to the second waste disposal device. The top of the sample rack 135 is provided with a plurality of sample tube sleeves 1351, and the sides of the plurality of sample tube sleeves 1351 are provided with barcode scanning ports 1352.

[0065] Specifically, the sample to be tested is placed into the sample tube sleeve 1351 of the sample rack 135, with the barcode of the sample to be tested aligned with the barcode scanning port 1352. The sample rack 135 is then placed in the sample inlet 133. The slots on the sample rack 135 engage and slide with the barcodes provided in the sample transfer mechanism 13. The sample rack 135 is then transferred to the sample loading area 131 via the first sensing mechanism 137 and the first drive mechanism. The barcode scanner 1313 transmits the information of the sample to be tested to the incubation system. After the sample to be tested in the sample rack 135 is added to the test tube, the first sensing mechanism 137 and the first drive mechanism transfer the sample rack 135 from the sample loading area entrance 13211 to the first sample buffer 1321. When the first sample buffer 1321 is full of sample racks 135, the sample rack 135 is transferred from the sample buffer entrance 13213 to the second sample buffer 1322. Finally, a staff member removes the completed test tube rack from the sample buffer 132.

[0066] The test tube moving mechanism 12 uses a first telescopic rod 124 to drive a second telescopic rod 125 to slide left and right on the sliding mechanism 121. Simultaneously, the first and second telescopic rods 124, 125 move up and down, and forward and backward, moving the test tube holder 126 into the test tube rack storage area 10 and aligning it with the test tube. The test tube moving mechanism 12 then moves the test tube to a designated position in the sample incubation mechanism 14. The test tube moving mechanism 12 reciprocates on the sliding mechanism 121, enabling automated and precise test tube retrieval.

[0067] like Figure 1-11 As shown, the first sensing mechanism 137 further includes a first sample rack baffle 1371 and a second sample rack baffle 1372 . The first sample rack baffle 1371 is disposed at the sample loading area entrance 13211 , and the second sample rack baffle 1372 is disposed at the sample buffer area entrance 13213 .

[0068] A third sample rack slot 1355 is defined on opposite sides of the sample rack 135. A first sample rack slot 1353 and a second sample rack slot 1354 are symmetrically defined at the bottom of the sample rack 135. A first sample rack clip 1314 and a second sample rack clip 1315 are provided at the bottoms of both the sample inlet area 133 and the sample buffer area 132. A third sample rack clip 1316 is provided on the sides of both the sample inlet area 133 and the sample buffer area 132. The first sample rack slot 1353 is engaged and slidably connected to the first sample rack clip 1314, the second sample rack slot 1354 is engaged and slidably connected to the second sample rack clip 1315, and the third sample rack slot 1355 is engaged and slidably connected to the third sample rack clip 1316.

[0069] Specifically, the first sample rack slot 1353 is engaged and slidably connected to the first sample rack clip 1314, the second sample rack slot 1354 is engaged and slidably connected to the second sample rack clip 1315, and the third sample rack slot 1355 is engaged and slidably connected to the third sample rack clip 1316, thereby forming a stable connection for the sample rack 135 and achieving precise movement of the sample rack 135 during movement.

[0070] When sample rack 135 is placed in sample loading area 133, it contacts the conveyor belt of conveyor mechanism 136. The pressure sensor of first sensing mechanism 137 senses pressure and transmits a signal to the transmission system, controlling the conveyor belt to move leftward. When sample rack 135 contacts first sample rack baffle 1371, the pressure sensor on that baffle senses pressure and transmits a signal to the transmission system, controlling the conveyor belt to stop. Simultaneously, sample rack position limiting unit 134 moves rightward to a designated position. First sample rack baffle 1371 moves inward, causing sample rack position limiting unit 134 to move upward 0.5 mm, reducing friction between sample rack 135 and the conveyor belt. Sample rack position limiting unit 134 then moves leftward, carrying sample rack 135 to sample loading area entrance 13211. The sample rack then moves backward and enters sample loading area 131 through sample loading area entrance 13211.

[0071] After the sample to be tested on the sample rack 135 is loaded, the sample rack limit moving unit 134 moves forward with the sample rack 135, and then moves to the left, and moves the sample rack 135 to the designated position in the sample buffer area 132, and touches the second sample rack baffle 1372. When the pressure sensor device senses the pressure, it transmits the signal to the system, controls the sample rack limit moving unit 134 to stop moving to the left, and controls the second sample rack baffle 1372 to move inward, starts the conveyor belt of the first sample buffer area 1321, and moves the sample to the designated position in the sample buffer area 132. When the sample storage in the first sample buffer area 1321 is full, the sample rack limiting moving unit 134 moves the sample rack 135 toward the sample buffer area 132, touching the second sample rack baffle 1372. When the pressure sensing device senses the pressure, it transmits a signal to the system to control the sample rack limiting moving unit 134 to stop moving to the left and start moving backward, transferring the sample through the sample buffer area entrance 13213 to the second sample buffer area 1322, waiting for the tester to take the tested sample.

[0072] like Figure 1-11As shown, the sample incubation mechanism 14 includes an incubation chamber 141, an incubation tray 142, a reagent storage chamber 143, a sample mixing mechanism 144, a second sensing mechanism, a second driving mechanism, an incubation chamber cover 147, a third driving mechanism and a third sensing mechanism. The incubation tray 142 is arranged in the incubation chamber 141, and a through hole is opened in the center of the incubation tray 142. The reagent storage chamber 143 passes through the through hole and is placed in the through hole. A reagent position 1431 is provided in the reagent storage chamber 143, and a second sensing mechanism and a second driving mechanism are provided in the incubation chamber 141. The incubation chamber cover 147 is arranged above the incubation chamber 141, and a second test tube hole 1471, a second sample addition hole 1472 and a third test tube hole 1473 are opened on the incubation chamber cover 147. There are three sample loading holes 1473, a reagent storage chamber cover 1432 is provided above the reagent storage chamber 143, a fourth sample loading hole 1433 is provided on the reagent storage chamber cover 1432, the sample mixing mechanism 144 is correspondingly provided below the third sample loading hole 1473, a third driving mechanism and a third sensing mechanism are provided below the sample mixing mechanism 144, a sealing strip 1474 is provided on the incubation chamber cover 147 and the reagent storage chamber cover 1432, a plurality of limiting members 1475 are provided on the incubation chamber cover 147 and the reagent storage chamber cover 1432, a plurality of limiting grooves 1411 are provided on the side walls of the incubation chamber 141 and the reagent storage chamber 143, and the plurality of limiting members 1475 are adapted to be connected with the plurality of limiting grooves 1411.

[0073] Specifically, a second sensing mechanism provided in the incubation chamber 141 maintains a constant temperature for the incubation tray 142 and the reaction samples. In this embodiment, the incubation chamber 141 and the incubation chamber cover 147 are connected to the plurality of retaining grooves 1411 via a plurality of retaining members 1475 and are sealed by a sealing strip 1474. The reagent storage chamber 143 and the reagent storage chamber cover 1432 are connected to the plurality of retaining grooves 1411 via a plurality of retaining members 1475 and are sealed by a sealing strip 1474.

[0074] In this embodiment, the second sensing mechanism comprises a temperature control device and a temperature sensing device. After the test tube moving mechanism 12 transfers the test tube to the second test tube well 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, transferring the test tube to be tested to below the second sample loading well 1472. The loading mechanism 15 draws the sample to be tested from the first sample loading well 1311 and adds it to the test tube to be tested through the second sample loading well 1472. The second driving mechanism drives the incubation tray 142 to rotate, transferring the test tube to be tested to below the third sample loading well 1473. The loading mechanism 15 adds the reaction solution reagent in the fourth sample loading well 1433 to the test tube to be tested below the third sample loading well 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and reaction liquid reagent in the test tube through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the sample adding mechanism 15 adds the dye in the fourth sample adding hole 1433 to the test tube to be tested below the third sample adding hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, reaction liquid and dye in the test tube through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0075] like Figure 1-11 As shown, the sample loading mechanism 15 has a first sample loading mechanism 151 and a second sample loading mechanism 152. The sample loading mechanism 15 includes a third telescopic rod 153, a rotating arm 154, a motor and a sample loading needle 155. The lower end of the third telescopic rod 153 is provided with a gear, and the gear at the lower end of the third telescopic rod 153 is connected to the gear of the motor. The upper end of the third telescopic rod 153 is fixedly connected to one end of the rotating arm 154, and the other end of the rotating arm 154 is fixedly connected to the sample loading needle 155. The upper end of the sample loading needle 155 is connected to a washing liquid tube and a pressure tube, the other end of the pressure tube is connected to a pressure pump, and the other end of the washing liquid tube is connected to the washing liquid bucket 17 and the pressure pump. The ends of the washing liquid tubes are all connected to sensing devices.

[0076] Specifically, the second driving mechanism drives the incubation plate 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample loading hole 1472. The first sample loading mechanism 151 starts to work, and the motor controls the third telescopic rod 153, the rotating arm 154 and the sample loading needle 155 to rotate. When the sample loading needle 155 rotates to above the first sample loading hole 1311, the motor stops working, and the third telescopic rod 153 moves downward, so that the sample loading needle 155 enters the sample; the pressure pump works, and a negative pressure is generated in the inner cavity of the sample loading needle 155 through the pressure tube, and the sample enters the sample loading needle 155; the third telescopic rod 153 moves upward, and the sample loading needle 155 and the sample move upward. The first loading mechanism 151 moves to the designated position. The motor controls the sample needle 155 of the first loading mechanism 151 to rotate above the second loading well 1472. The third telescopic rod 153 moves downward, allowing the sample needle 155 to enter the test tube in the incubation tray 142. The pressure pump operates to generate positive pressure in the inner cavity of the sample needle 155, adding the sample to the test tube. The third telescopic rod 153 moves upward, carrying the sample needle 155 to the designated position. The motor controls the sample needle 155 of the first loading mechanism 151 to rotate to the cleaning well. The pressure pump connected to the cleaning fluid tube begins to operate, injecting the cleaning fluid from the cleaning fluid barrel 17 into the sample needle 155, cleaning the sample needle 155. The second drive mechanism drives the incubation tray 142 to rotate, transferring the test tube to be tested to the bottom of the third loading well 1473. The sample needle 155 of the second loading mechanism 152 adds the reaction solution reagent in the fourth loading well 1433 to the test tube to be tested below the third loading well 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and reaction liquid reagent in the test tube through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the loading needle 155 of the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested below the third loading hole 1473. After the sample, reaction liquid and dye in the test tube of the sample to be tested are mixed evenly through the third driving mechanism, the incubation is continued for 10 minutes to complete the incubation.

[0077] like Figure 1-11 As shown, the second waste disposal device includes a waste storage box 161 and a waste liquid barrel 162 . The bottom of the first test tube hole 1312 is connected to the waste storage box 161 , and the bottom of the first sample loading mechanism 151 is connected to the waste liquid barrel 162 and the washing liquid barrel 17 .

[0078] Specifically, after the first loading mechanism 151 and the second loading mechanism 152 complete the loading, the motor controls the loading needle 155 of the first loading mechanism 151 to rotate to the cleaning hole, and the pressure pump connected to the washing liquid tube starts working, injecting the washing liquid in the washing liquid barrel 17 into the loading needle 155, cleaning the loading needle 155, and discharging the waste liquid into the waste liquid barrel 162.

[0079] The beneficial effect of this embodiment is that the fully automated and precise sample loading and incubation system enables precise sample loading by automated instruments. The sample is mixed with an oxidizing sample reaction solution in the sample incubation mechanism 14 and incubated to oxidize and denature the unstable hemoglobin and precipitate it into insoluble Heinz bodies, which are then stained with a dye to turn the Heinz bodies purple. The automated incubation system improves work efficiency, standardizes experimental operations, and ensures the quality of experimental results.

[0080] The working process of this embodiment is as follows: the sample to be tested is placed in the sample tube sleeve 1351 of the sample rack 135, with the barcode of the sample to be tested facing the barcode scanning port 1352. The sample rack 135 is placed in the sample inlet area 133. The first sample rack slot 1353 is engaged and slidably connected with the first sample rack clip 1314, the second sample rack slot 1354 is engaged and slidably connected with the second sample rack clip 1315, and the third sample rack slot 1355 is engaged and slidably connected with the third sample rack clip 1316. When the sample rack 135 is placed in the sample inlet area 133, it touches the conveyor belt of the conveying structure 136. When the pressure sensor device of the first sensing mechanism 137 senses pressure, it transmits a signal to the system to control the conveyor belt to move to the left. When the sample rack 135 touches the first sample rack baffle 1371, the pressure sensor on the first sample rack baffle 1371 senses the pressure and transmits a signal to the system, controlling the conveyor belt to stop working and simultaneously controlling the sample rack limit moving unit 134 to move rightward to the specified position. The first sample rack baffle 1371 moves inward, and the sample rack limit moving unit 134 moves upward by 0.5 mm, reducing the friction between the sample rack 135 and the conveyor belt. The sample rack limit moving unit 134 moves leftward, carrying the sample rack 135 to the sample loading area entrance 13211, and then moves backward and enters the sample loading area 131 through the sample loading area entrance 13211.

[0081] After the sample to be tested on the sample rack 135 is loaded, the sample rack limit moving unit 134 moves forward with the sample rack 135, and then moves to the left, and moves the sample rack 135 to the designated position in the sample buffer area 132, and touches the second sample rack baffle 1372. When the pressure sensor device senses the pressure, it transmits the signal to the system, controls the sample rack limit moving unit 134 to stop moving to the left, and controls the second sample rack baffle 1372 to move inward, starts the conveyor belt of the first sample buffer area 1321, and moves the sample to the designated position in the sample buffer area 132. When the sample storage in the first sample buffer area 1321 is full, the sample rack limiting moving unit 134 moves the sample rack 135 toward the sample buffer area 132, touching the second sample rack baffle 1372. When the pressure sensing device senses the pressure, it transmits a signal to the system to control the sample rack limiting moving unit 134 to stop moving to the left and start moving backward, transferring the sample through the sample buffer area entrance 13213 to the second sample buffer area 1322, waiting for the tester to take the tested sample.

[0082] The test tube moving mechanism 12 uses the first telescopic rod 124 to drive the second telescopic rod 125 to slide left and right on the sliding mechanism 121. Simultaneously, the first and second telescopic rods 124, 125 move up and down, and forward and backward, moving the test tube holder 126 to the test tube rack storage area 10 and aligning it with the test tube. The test tube moving mechanism 12 then moves the test tube to the second test tube hole 1471 of the sample incubation mechanism 14; the test tube moving mechanism 12 can reciprocate on the sliding mechanism 121.

[0083] After the test tube moving mechanism 12 transfers the test tube to the second test tube hole 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample loading hole 1472. The first sample loading mechanism 151 draws the sample to be tested from the first sample loading hole 1311 and adds it to the test tube to be tested through the second sample loading hole 1472. The second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample loading hole 1473. The second sample loading mechanism 152 adds the reaction liquid reagent in the fourth sample loading hole 1433 to the test tube to be tested below the third sample loading hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and reaction liquid reagent in the test tube through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested below the third loading hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, reaction liquid and dye in the test tube through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0084] Example 2

[0085] like Figure 12As shown, based on Example 1, an incubation method of a Heinz body incubation system is:

[0086] S1: The Heinz body incubation system is automatically reset, and the second sensing mechanism of the sample transfer mechanism 13 controls the temperature to 37°C, the temperature required for the sample reaction; the tester empties the waste storage box 161 and the waste liquid bucket 162, places a test tube rack containing clean test tubes in the test tube rack storage area 10, and fills the washing liquid bucket 17 with washing liquid;

[0087] S2: Place the sample to be tested into the sample tube sleeve 1351 of the sample rack 135, with the barcode of the sample to be tested facing the barcode scanning port 1352. The sample rack 135 is then placed in the sample inlet area 133. The sample rack 135 is then slidably connected to the sample transfer mechanism 13 by engaging the card slot with the card strip. The gravity of the sample rack 135 acts on the conveying structure 136. The first sensing mechanism 137 transmits the received signal to the first driving mechanism. The first driving mechanism drives the conveying structure 136 to move the sample rack 135. The sample rack limit moving unit 134 controls the sample rack 135 to move from the sample inlet area 133 to the sample adding area 131.

[0088] S3: The barcode scanner 1313 scans the barcode of the sample to be tested and transmits the sample information to the incubation system. The incubation system controls the test tube moving mechanism 12 to work, and the test tube moving mechanism 12 transfers the test tube from the test tube rack storage area 10 to the sample incubation mechanism 14;

[0089] S4: The second sensing mechanism of the sample incubation mechanism 14 senses that the test tube enters the incubation chamber 141, and the second driving mechanism drives the incubation tray 142 to rotate;

[0090] S5: The first loading mechanism 151 draws the sample from the first loading hole 1311 and adds the sample into the test tube through the second loading hole 1472. Then, the loading needle 155 of the first loading mechanism 151 is cleaned with a washing solution.

[0091] S6: The second loading mechanism 152 draws the reaction liquid reagent from the fourth loading hole 1433 and adds the reaction liquid reagent to the test tube through the third loading hole 1473. The sample in the test tube and the reaction liquid reagent are mixed evenly and incubated. Then, the loading needle 155 of the second loading mechanism 152 is cleaned with a washing solution.

[0092] S7: After all the samples to be tested are added to the sample rack 135, the sample rack limiting moving unit 134 moves the sample rack 135 and moves the sample rack 135 to a designated position in the sample buffer area 132;

[0093] S8: After the sample and reaction solution reagent in the test tube are evenly mixed and incubated, the second loading mechanism 152 draws the dye from the fourth loading well 1433 and adds the dye to the test tube through the third loading well 1473. Simultaneously, the sample mixing mechanism 144 mixes the sample to be tested, the reaction solution reagent, and the dye sample in the test tube and incubates. Then, the loading needle 155 of the second loading mechanism 152 is cleaned with a wash solution.

[0094] S9: After the sample has been incubated for 10 minutes, the second drive mechanism drives the incubation tray 142 to rotate and rotate the test tube containing the sample to be tested to below the third loading well 1473. The loading needle 155 of the second loading mechanism 152 is positioned above the third loading well 1473 and the sample to be tested is sampled and loaded onto the slide. The loading needle 155 of the second loading mechanism 152 is then cleaned with a wash solution.

[0095] S10: After the reagent incubated in the sample test tube is sampled and tested, the second driving mechanism drives the incubation disk 142 to rotate and move the test tube to the second test tube hole 1471. The test tube moving mechanism 12 removes the test tube from the second test tube hole 1471 and transfers it to the waste storage box 161 through the first test tube hole 1312.

[0096] S11: Repeat steps S1 to S10 multiple times according to the incubation requirements, and incubate in sequence.

[0097] The beneficial effect of adopting the above scheme is that through the fully automated precise sample addition and incubation of the incubation system, precise sample addition of the automated instrument can be achieved. The sample is mixed with the sample reaction liquid with an oxidizing effect in the sample incubation mechanism 14 and incubated to react, and the unstable hemoglobin is oxidized and denatured to precipitate into insoluble Heinz bodies, which are then stained with a dye to dye the Heinz bodies purple. A high-resolution electron microscope imaging system is further used for image acquisition, and the Heinz bodies are analyzed and detected by AI analysis technology, thereby realizing automated detection of the experiment, improving personnel work efficiency, and standardizing experimental operations, and ensuring the quality of experimental results.

[0098] The working process of this embodiment is as follows: the power switch is turned on, the Heinz body detector automatically resets, and the temperature control system of the sample incubation mechanism 14 controls the temperature to 37°C, the temperature required for the sample reaction; the tester empties the waste storage box 161 and the waste liquid barrel 162, places a test tube rack containing clean test tubes in the test tube rack storage area 10, and fills the washing liquid barrel 17 with washing liquid.

[0099] The sample to be tested is placed in the sample tube sleeve 1351 of the sample rack 135, with the barcode of the sample to be tested facing the barcode scanning port 1352. The sample rack 135 is placed in the sample inlet area 133. The first sample rack slot 1353 is engaged and slidably connected with the first sample rack clip 1314, the second sample rack slot 1354 is engaged and slidably connected with the second sample rack clip 1315, and the third sample rack slot 1355 is engaged and slidably connected with the third sample rack clip 1316. When the sample rack 135 is placed in the sample inlet area 133, it touches the conveyor belt of the conveying structure 136. When the pressure sensor device of the first sensing mechanism 137 senses pressure, it transmits a signal to the system to control the conveyor belt to move to the left. When the sample rack 135 touches the first sample rack baffle 1371, the pressure sensor on the first sample rack baffle 1371 senses the pressure and transmits a signal to the system, controlling the conveyor belt to stop working and simultaneously controlling the sample rack limit moving unit 134 to move rightward to the specified position. The first sample rack baffle 1371 moves inward, and the sample rack limit moving unit 134 moves upward by 0.5 mm, reducing the friction between the sample rack 135 and the conveyor belt. The sample rack limit moving unit 134 moves leftward, carrying the sample rack 135 to the sample loading area entrance 13211, and then moves backward and enters the sample loading area 131 through the sample loading area entrance 13211.

[0100] The barcode scanner 1313 scans the barcode of the sample to be tested and transmits the information of the sample to be tested to the system. The system controls the test tube moving mechanism 12 to start working, and the sliding mechanism 121 of the test tube moving mechanism 12 works. The first telescopic rod 124, the second telescopic rod 125 and the test tube sleeve 126 move left and right in the sliding mechanism 121; the first telescopic rod 124, the second telescopic rod 125 and the test tube sleeve 126 move left to the test tube rack storage area 10, locking the test tube position, the first telescopic rod 124 moves upward, and the second telescopic rod 125 moves forward, and the test tube sleeve 126 moves left to the test tube rack storage area 10. 126 moves to above the test tube, the first telescopic rod 124 moves downward, the test tube enters the entrance and enters the test tube sleeve 126, when the sensing detection device 1267 in the test tube sleeve 126 senses the test tube, a signal is transmitted to the system, the first telescopic rod 124 is controlled to stop moving downward, and the test tube sleeve 126 is controlled to clamp the test tube, and a signal is transmitted to the system, thereby controlling the sliding mechanism 121 of the test tube moving mechanism 12 to work in the reverse direction, driving the first telescopic rod 124, the second telescopic rod 125, the test tube sleeve 126 and the test tube to move right to the sample incubation mechanism 14.

[0101] After the samples to be tested in the sample rack 135 are added to the test tubes, the first sensing mechanism 137 and the first driving mechanism transfer the sample rack 135 from the sample loading area entrance 13211 to the first sample buffer area 1321. When the first sample buffer area 1321 is full of sample racks 135, the sample rack 135 is transferred from the sample buffer area entrance 13213 to the second sample buffer area 1322. Finally, the staff removes the completed test tube racks from the sample buffer area 132.

[0102] After the test tube moving mechanism 12 transfers the test tube to the second test tube hole 1471 of the incubation tray 142, the second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the second sample loading hole 1472. The first sample loading mechanism 151 draws the sample to be tested from the first sample loading hole 1311 and adds it to the test tube to be tested through the second sample loading hole 1472. The second driving mechanism drives the incubation tray 142 to rotate, and transfers the test tube to be tested to the bottom of the third sample loading hole 1473. The second sample loading mechanism 152 adds the reaction liquid reagent in the fourth sample loading hole 1433 to the test tube to be tested below the third sample loading hole 1473. Then, the test tube of the sample to be tested is mixed evenly with the sample and reaction liquid reagent in the test tube through the third driving mechanism, and then placed in the incubation tray 142 for constant temperature incubation at 37.0°C for 3.5 hours. After the incubation is completed, the second loading mechanism 152 adds the dye in the fourth loading hole 1433 to the test tube to be tested below the third loading hole 1473. The test tube of the sample to be tested is mixed evenly with the sample, reaction liquid and dye in the test tube through the third driving mechanism, and then continues to incubate for 10 minutes to complete the incubation.

[0103] After the sample to be tested on the sample rack 135 is loaded, the sample rack limit moving unit 134 moves forward with the sample rack 135, and then moves to the left, and moves the sample rack 135 to the designated position in the sample buffer area 132, and touches the second sample rack baffle 1372. When the pressure sensor device senses the pressure, it transmits the signal to the system, controls the sample rack limit moving unit 134 to stop moving to the left, and controls the second sample rack baffle 1372 to move inward, starts the conveyor belt of the first sample buffer area 1321, and moves the sample to the designated position in the sample buffer area 132. When the sample storage in the first sample buffer area 1321 is full, the sample rack limiting moving unit 134 moves the sample rack 135 toward the sample buffer area 132, touching the second sample rack baffle 1372. When the pressure sensing device senses the pressure, it transmits a signal to the system to control the sample rack limiting moving unit 134 to stop moving to the left and start moving backward, transferring the sample through the sample buffer area entrance 13213 to the second sample buffer area 1322, waiting for the tester to take out the tested sample.

[0104] After the reagent incubated in the sample test tube to be tested is sampled and tested, the second driving mechanism drives the incubation disk 142 to rotate, and rotates the test tube to the second test tube hole 1471. The test tube moving mechanism 12 takes the test tube out of the second test tube hole 1471 and transfers it to the waste storage box 161 through the first test tube hole 1312.

[0105] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0106] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0107] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0108] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0109] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, mechanisms, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, mechanisms, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0110] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A Heinz body incubation system, characterized in that: The incubation system comprises a test tube rack storage area (10), a test tube moving mechanism (12), a sample transfer mechanism (13), a sample incubation mechanism (14), a sample adding mechanism (15), a waste disposal device and a washing liquid bucket (17), wherein the incubation system is arranged in an incubation box (1), the test tube rack storage area (10) is arranged on the left side of the incubation box (1), the test tube rack storage area (10) is provided with a plurality of test tube rack placement positions (101), the test tube moving mechanism (12) is slidably arranged at the rear end of the test tube rack storage area (10), and the sample incubation mechanism (14) is arranged on the left side of the incubation box (1), and the sample incubation mechanism (15) is arranged on the rear end of the test tube rack storage area (10). The sample transfer mechanism (13) is arranged at the front end of the test tube rack storage area (10), the sample incubation mechanism (14) and the sample loading mechanism (15) are arranged on the right side of the incubator (1), the sample loading mechanism (15) is arranged on the sample incubation mechanism (14), and the waste disposal device and the washing liquid bucket (17) are arranged below the test tube rack storage area (10), the test tube moving mechanism (12), the sample transfer mechanism (13), the sample incubation mechanism (14) and the sample loading mechanism (15).

2. A Heinz body incubation system according to claim 1, characterized in that: The test tube moving mechanism (12) comprises a sliding mechanism (121), a first telescopic rod (124), a second telescopic rod (125) and a test tube sleeve (126); the lower end of the first telescopic rod (124) is fixedly arranged on the sliding mechanism (121); the upper end of the first telescopic rod (124) is fixedly connected to one end of the second telescopic rod (125); and the other end of the second telescopic rod (125) is fixedly connected to the test tube sleeve (126).

3. A Heinz body incubation system according to claim 2, characterized in that: The test tube sleeve (126) comprises a test tube sleeve opening (1261), a test tube clamp (1262), a test tube clamp telescopic rod (1263), a clamping piece (1264), a clamping piece support rod (1265), a spring (1266) and an induction detection device (1267). The interior of the test tube sleeve (126) is a hollow trapezoid, with a smaller upper end and a larger lower end. The lower end is the test tube sleeve opening (1261). The top of the test tube clamp (1262) The test tube clamp telescopic rod (1263) is provided, the bottom of the test tube clamp (1262) is provided with two clamping member support rods (1265), the bottom of the two clamping member support rods (1265) is respectively provided with a clamping member (1264), the two ends of the spring are respectively connected to the clamping member support rods (1265) on both sides, and the side wall of the test tube sleeve (1261) is also provided with an induction detection device (1267); The sliding mechanism (121) comprises a sliding rail (1211), a screw rod (1212), a threaded slider (1213), a mechanism gear (1214) and a motor; two ends of the screw rod (1212) are arranged on both sides of the sliding rail (1211); one end of the screw rod (1212) is provided with the mechanism gear (1214); the mechanism gear (1214) is connected to the output end of the motor; the threaded slider (1213) is fixedly arranged on the sliding rail (1211); the threaded slider (1213) is movably arranged on the screw rod (1212); and the first telescopic rod (124) is also fixedly arranged on the threaded slider (1213).

4. A Heinz body incubation system according to claim 1, characterized in that: The sample transfer mechanism (13) comprises a sample introduction area (131), a sample buffer area (132), a sample adding area (133), a sample rack limit moving unit (134), a sample rack (135), a conveying structure (136), a first sensing mechanism (137) and a first driving mechanism. The sample introduction area (131) is arranged on the right side of the sample transfer mechanism (13), the sample buffer area (132) is arranged on the left side of the sample transfer mechanism (13), and the sample buffer area (132) comprises a first The sample buffer area (1321) and the second sample buffer area (1322), the sample injection area (131) is connected to the sample addition area (133) through a sample addition area inlet (13211), the first sample buffer area (1321) and the second sample buffer area (1322) are connected through a sample buffer area inlet (13213), the bottom of the sample injection area (131), the sample buffer area (132) and the sample addition area (133) are all provided with the conveying structure (136), and the conveying structure (136) The first sensing mechanism (137) and the first driving mechanism are arranged at the bottom of the sample adding area entrance (13211) and the sample buffer area entrance (13213), and the sample rack (135) is slidably connected with the sample feeding area (131), the sample buffer area (132) and the sample rack limit moving unit (134); the sample rack limit moving unit (134) is composed of a PLC controller, and the sample feeding area (131) is connected to the sample buffer area (132). A first sample loading hole (1311) and a first test tube hole (1312) are provided on the top of the sample rack (135); a barcode scanner (1313) is provided on the side of the sample loading area (131) and at a position corresponding to the first sample loading hole (1311); the first test tube hole (1312) is connected to the waste disposal device below; a plurality of sample tube sleeves (1351) are provided on the top of the sample rack (135), and a barcode scanning port (1352) is provided on the side of the plurality of sample tube sleeves (1351).

5. A Heinz body incubation system according to claim 4, characterized in that: The first sensing mechanism (137) further comprises a first sample rack baffle (1371) and a second sample rack baffle (1372), wherein the first sample rack baffle (1371) is arranged at the entrance (13211) of the sample adding area, and the second sample rack baffle (1372) is arranged at the entrance (13213) of the sample buffer area; The sample rack (135) is provided with a third sample rack card slot (1355) on two opposite sides thereof; the bottom of the sample rack (135) is symmetrically provided with a first sample rack card slot (1353) and a second sample rack card slot (1354); the bottoms of the sample injection area (131) and the sample buffer area (132) are both provided with a first sample rack card strip (1314) and a second sample rack card strip (1315); the sides of the sample injection area (131) and the sample buffer area (132) are both provided with a third sample rack card strip (1316); the first sample rack card slot (1353) is slidably connected to the first sample rack card strip (1314); the second sample rack card slot (1354) is slidably connected to the second sample rack card strip (1315); and the third sample rack card slot (1355) is slidably connected to the third sample rack card strip (1316).

6. A Heinz body incubation system according to claim 1, characterized in that: The sample incubation mechanism (14) comprises an incubation chamber (141), an incubation tray (142), a reagent storage chamber (143), a sample mixing mechanism (144), a second sensing mechanism, a second driving mechanism, an incubation chamber cover (147), a third driving mechanism and a third sensing mechanism. The incubation tray (142) is arranged in the incubation chamber (141). A through hole is provided in the center of the incubation tray (142). The reagent storage chamber (143) passes through the through hole and is placed in the through hole. A reagent position (1431) is provided in the reagent storage chamber (143). The incubation chamber (141) is provided with a second sensing mechanism and a second driving mechanism. The incubation chamber cover (147) is arranged above the incubation chamber (141). A second test tube hole (1471), a second sample loading hole (1472) and a third sample loading hole (1473) are provided on the incubation chamber cover (147). 1473), a reagent storage chamber cover (1432) is arranged above the reagent storage chamber (143), a fourth sample loading hole (1433) is opened on the reagent storage chamber cover (1432), the sample mixing mechanism (144) is arranged correspondingly below the third sample loading hole (1473), the third driving mechanism and the third sensing mechanism are arranged below the sample mixing mechanism (144), a sealing strip (1474) is arranged on the incubation chamber cover (147) and the reagent storage chamber cover (1432), a plurality of limiting members (1475) are arranged on the incubation chamber cover (147) and the reagent storage chamber cover (1432), a plurality of limiting grooves (1411) are opened on the side walls of the incubation chamber (141) and the reagent storage chamber (143), and a plurality of limiting members (1475) are adapted to be connected with a plurality of limiting grooves (1411).

7. A Heinz body incubation system according to claim 4, characterized in that: The sample loading mechanism (15) comprises a first sample loading mechanism (151) and a second sample loading mechanism (152). The sample loading mechanism (15) comprises a third telescopic rod (153), a rotating arm (154), a motor and a sample loading needle (155). A gear is arranged at the lower end of the third telescopic rod (153). The gear at the lower end of the third telescopic rod (153) is connected to the gear of the motor. The upper end of the third telescopic rod (153) is fixedly connected to one end of the rotating arm (154). The other end of the rotating arm (154) is fixedly connected to the sample loading needle (155). The upper end of the sample loading needle (155) is connected to a washing liquid tube and a pressure tube. The other end of the pressure tube is connected to a pressure pump. The other end of the washing liquid tube is connected to a washing liquid bucket (17) and the pressure pump. The ends of the washing liquid tubes are connected to sensing devices.

8. A Heinz body incubation system according to claim 7, characterized in that: The waste disposal device comprises a waste storage box (161) and a waste liquid barrel (162); the bottom of the first test tube hole (1312) is connected to the waste storage box (161); and the bottom of the first sample adding mechanism (151) is connected to the waste liquid barrel (162) and the washing liquid barrel (17).

9. A Heinz body incubation system according to claim 1, characterized in that: An incubator cover (11) is provided on the top of the incubator (1), and the incubator cover (11) is hinged to one side of the incubator (1).

10. A method for incubating Heinz bodies, characterized in that: A Heinz body incubation system according to any one of claims 1 to 9, wherein the incubation method is: S1: The Heinz body incubation system is automatically reset, and the second sensing mechanism of the sample transfer mechanism (13) controls the temperature to 37° C., a temperature required for the sample reaction; the test personnel empties the waste storage box (161) and the waste liquid bucket (162), puts a test tube rack containing clean test tubes in the test tube rack storage area (10), and fills the washing liquid bucket (17) with washing liquid; S2: placing the sample to be tested into the sample tube sleeve (1351) of the sample rack (135), with the barcode of the sample to be tested facing the barcode scanning port (1352), and placing the sample rack (135) into the sample injection area (133), and engaging and slidingly connecting the sample rack (135) with the sample transfer mechanism (13) by engaging the card slot with the card strip, the gravity of the sample rack (135) acts on the transmission structure (136), and the first sensing mechanism (137) transmits the received signal to the first driving mechanism, and the first driving mechanism drives the transmission structure (136) to move the sample rack (135), and the sample rack limit moving unit (134) controls the sample rack (135) to enter the sample addition area (133) from the sample injection area (131); S3: Scanning the barcode of the sample to be tested by the barcode scanner (1313), transmitting the information of the sample to be tested to the incubation system, and the incubation system controls the test tube moving mechanism (12) to work, and the test tube moving mechanism (12) transfers the test tube from the test tube rack storage area (10) to the sample incubation mechanism (14); S4: the second sensing mechanism of the sample incubation mechanism (14) senses that the test tube has entered the incubation chamber (141), and the second driving mechanism drives the incubation plate (142) to rotate; S5: the first sample loading mechanism (151) draws the sample from the first sample loading hole (1311), and adds the sample into the test tube through the second sample loading hole (1472), and then washes the sample loading needle (155) of the first sample loading mechanism (151) with a washing liquid; S6: The second loading mechanism (152) draws the reaction liquid reagent from the fourth loading hole (1433), and adds the reaction liquid reagent into the test tube through the third loading hole (1473), and mixes the sample in the test tube with the reaction liquid reagent and incubates them, and then washes the loading needle (155) of the second loading mechanism (152) with a washing solution; S7: After all samples to be tested are added to the sample rack (135), the sample rack position limiting moving unit (134) moves the sample rack (135) and moves the sample rack (135) to a designated position of the sample buffer area (132); S8: After the sample in the test tube and the reaction liquid reagent are mixed evenly and incubated, the second sample adding mechanism (152) draws the dye from the fourth sample adding hole (1433) and adds the dye into the test tube through the third sample adding hole (1473). At the same time, the sample mixing mechanism (144) mixes the sample to be tested, the reaction liquid reagent and the sample of the dye in the test tube and incubates them. Then, the sample adding needle (155) of the second sample adding mechanism (152) is cleaned with a washing solution. S9: After the sample is incubated for 10 minutes, the second driving mechanism drives the incubation plate (142) to rotate, and rotates the test tube containing the sample to be tested to the bottom of the third sample loading hole (1473), and the sample loading needle (155) of the second sample loading mechanism (152) is positioned above the third sample loading hole (1473), and the sample to be tested is sampled and added to the slide; then, the sample loading needle (155) of the second sample loading mechanism (152) is cleaned with a washing solution; S10: After the reagent incubated in the sample test tube to be tested is sampled and tested, the second driving mechanism drives the incubation plate (142) to rotate, and the test tube is rotated to the second test tube hole (1471), and the test tube moving mechanism (12) takes the test tube out of the second test tube hole (1471) and transfers it to the waste storage box (161) through the first test tube hole (1312); S11: Repeat steps S1-S10 multiple times according to the incubation requirements, and incubate in sequence.

Images