Application of Prussella aryabhattai ZZRQ1 and combined modified zeolite thereof in nitrogen-containing sewage treatment

Abstract

The invention belongs to the technical field of biological agents and sewage treatment, and particularly relates to application of priesta aryabhattai ZZRQ1 (Priesta aryabhattai) ZZRQ1 and zeolite modified by the priesta aryabhattai ZZRQ1 in nitrogen-containing sewage treatment. According to the present invention, the P.adami ZZRQ1 has efficient heterotrophic nitrification performance, and can effectively remove NH4 < + >-N in the natural water body; the modified zeolite is used as a microbial carrier, the specific surface area of the zeolite can be increased through modification, the number of functional groups on the surface of the carrier is increased, the ammonia nitrogen removal capacity of the carrier is improved, more attachment interfaces are provided for microorganisms, and the adsorption performance of the modified zeolite is combined with the degradation performance of the Prussella aryabhattai ZZRQ1, so that the degradation performance of the Prussella aryabhattai ZZRQ1 is improved. Compared with unmodified zeolite, the modified zeolite has better capability of removing ammonia nitrogen in sewage.

Description

Technical Field

[0001] The invention belongs to the technical field of biological microbial agents and sewage treatment, and specifically relates to application of Priesteria axyri ZZRQ1 and a combined modified zeolite thereof in nitrogen-containing sewage treatment. Background Art

[0002] In recent years, the eutrophication of urban and rural rivers and lakes has become increasingly prominent. Excessive nitrogen in water bodies is one of the main causes of this phenomenon. The control of nitrogen pollution is considered to be the focus and difficulty of current water ecological restoration work. As a microbial treatment technology, the biofilm method is widely used in the removal of nitrogen and phosphorus and heavy metals from industrial wastewater. Compared with traditional denitrification methods, the biofilm method has the advantages of higher resistance to ammonia nitrogen shock load and less residual sludge. In recent years, the biofilm method has gradually begun to be used in the restoration of polluted river water bodies. However, in the process of actually applying the biofilm method to the restoration of polluted river water bodies, the added biological agents have the problem of difficulty in survival in the early stage, and the agents are easily lost in large quantities with the water flow.

[0003] In some studies, biological agents are loaded on biological carriers to improve the fixation ability of biological carriers to strains and avoid large-scale loss of biological agents. However, after combining existing biological agents with carriers such as zeolite, their ability to remove ammonia nitrogen is still poor and cannot meet the requirements of existing polluted water treatment. Summary of the invention

[0004] The purpose of the present invention is to provide an application of Priesteria axyri ZZRQ1 and a combined modified zeolite in the treatment of nitrogen-containing sewage. The present invention combines the degradation performance of the heterotrophic nitrifying bacteria Priesteria axyri ZZRQ1 with the adsorption performance of the modified zeolite to better remove ammonia nitrogen in sewage.

[0005] The present invention provides Priestia aryabhattai ZZRQ1, and the deposit number of Priestia aryabhattai ZZRQ1 is CGMCC No.27544.

[0006] The present invention also provides a bacterial agent containing Priesteria afroicola ZZRQ1 described in the above technical solution, wherein the bacterial agent comprises Priesteria afroicola ZZRQ1 and modified zeolite, and the Priesteria afroicola ZZRQ1 is loaded on the surface of the modified zeolite.

[0007] Preferably, based on the bacterial suspension of A. australis ZZRQ1, the mass ratio of the volume of the bacterial suspension of A. australis ZZRQ1 to the modified zeolite is 1-5 mL: 0.5-2 g, and the OD of the bacterial suspension of A. australis ZZRQ1 is 600The values ​​are 0.185~0.733.

[0008] Preferably, the modified zeolite comprises acid-modified zeolite or metal-modified zeolite;

[0009] The preparation method of the acid modified zeolite comprises: mixing the zeolite with a sulfuric acid solution having a mass concentration of 5%, stirring at 100° C. for 2 hours, washing the obtained modified zeolite to neutrality, and obtaining the acid modified zeolite;

[0010] The preparation method of the metal-modified zeolite comprises: mixing zeolite, ferrous sulfate and water, stirring at 30° C. for 2 hours, and drying the obtained modified zeolite to obtain the metal-modified zeolite.

[0011] Preferably, during the preparation of the acidic modified zeolite, the volume ratio of the zeolite mass to the sulfuric acid solution is 1 g:4 mL.

[0012] Preferably, in the preparation process of the metal-modified zeolite, the mass ratio of the zeolite to ferrous sulfate is 20:1, and the volume ratio of the mass of the zeolite to water is 125 g:500 mL.

[0013] The present invention also provides a method for preparing the bacterial agent described in the above technical solution, comprising the following steps: mixing a bacterial suspension of Priesteria axyri ZZRQ1 with modified zeolite and adsorbing the mixture under oscillation conditions for 24 to 36 hours to obtain the bacterial agent.

[0014] The present invention also provides the use of Priesteria axytogenes ZZRQ1 described in the above technical solution or the bacterial agent described in the above technical solution or the bacterial agent prepared by the preparation method described in the above technical solution in the treatment of nitrogen-containing wastewater.

[0015] The present invention also provides a method for treating nitrogen-containing wastewater, comprising any one of the following two items:

[0016] 1) mixing the bacterial agent described in the above technical solution or the bacterial agent prepared by the preparation method described in the above technical solution with nitrogen-containing wastewater and treating the mixture for 6 to 27 hours;

[0017] 2) The bacterial suspension of Priesteria axotris ZZRQ1, modified zeolite and nitrogen-containing wastewater are mixed and treated for 6 to 27 hours; the deposit number of Priesteria axotris ZZRQ1 is CGMCC No.27544.

[0018] Preferably, in 1), based on the mass of the modified zeolite in the bacterial agent, the volume ratio of the mass of the modified zeolite to the nitrogen-containing sewage is 1-5 g: 50-200 mL;

[0019] In the above 2), the OD of the bacterial suspension of Priesteria axyri ZZRQ1 was 600The value is 0.185-0.733; the volume ratio of the bacterial suspension of Apricot Klebsiella ZZRQ1 to the nitrogen-containing sewage is 1:20.

[0020] Beneficial effects:

[0021] The present invention provides a Priestia aryabhattai ZZRQ1, the deposit number of the Priestia aryabhattai ZZRQ1 is CGMCC No. 27544. The Priestia aryabhattai ZZRQ1 of the present invention has high-efficiency heterotrophic nitrification performance and can effectively remove NH4 + -N.

[0022] On this basis, the present invention also provides a bacterial agent containing Priesteria avorei ZZRQ1 described in the above technical solution, the bacterial agent includes Priesteria avorei ZZRQ1 and modified zeolite, and the Priesteria avorei ZZRQ1 is loaded on the surface of the modified zeolite. The present invention uses modified zeolite as a microbial carrier, and the modification can increase the specific surface area of ​​zeolite, increase the number of functional groups on the carrier surface, increase the carrier's ability to remove ammonia nitrogen, provide more attachment interfaces for microorganisms, and combine the adsorption performance of modified zeolite with the degradation performance of Priesteria avorei ZZRQ1, so that it can have a better ability to remove ammonia nitrogen in sewage than unmodified zeolite, and provide technical support for the treatment of polluted water bodies.

[0023] Biological Deposit Description

[0024] Priestia aryabhattai ZZRQ1, biological classification (Priestia aryabhattai), was deposited in the China General Microbiological Culture Collection on June 5, 2023. The deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Building 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 27544. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0026] Figure 1 This is the SEM image of the zeolite surface before modification in Example 1;

[0027] Figure 2 is the SEM image of the surface of the acid-modified zeolite in Example 1;

[0028] Figure 3 This is the SEM image of the metal-modified zeolite surface in Example 1;

[0029] Figure 4is the growth curve of Priesteria afroides ZZRQ1 in Example 1;

[0030] Figure 5 This is a diagram showing the effect of carrier Priesteria axytogenes ZZRQ1 under different adsorption time conditions in Example 1;

[0031] Figure 6 This is a diagram showing the removal effect of ammonia nitrogen by zeolite before and after combined modification with Priesteria axyti ZZRQ1 in Example 1;

[0032] Figure 7 is the aerated bioreactor used in Example 3, Figure 7 The dimensions of the reactor are in cm;

[0033] Figure 8 This is a diagram showing the treatment effect of domestic sewage by acid-modified zeolite loaded with Priesteria afroides ZZRQ1 in Example 3. DETAILED DESCRIPTION

[0034] The present invention provides Priestia aryabhattai ZZRQ1, and the deposit number of the Priestia aryabhattai ZZRQ1 is CGMCC No. 27544. The Priestia aryabhattai ZZRQ1 of the present invention is separated from riverbed mud, and the colony characteristics on the LB solid culture medium are off-white, round, and have clear edges; through 16S rRNA sequence determination and homology comparison, it is found that the similarity between the ZZRQ1 and Priestia aryabhattai is 99.86%, thereby confirming its biological classification and conducting biological preservation.

[0035] The present invention also provides a bacterial agent containing the Priesteria axeterii ZZRQ1 described in the above technical solution, wherein the bacterial agent includes Priesteria axeterii ZZRQ1 and a modified zeolite, and the Priesteria axeterii ZZRQ1 is loaded on the surface of the modified zeolite. Based on the bacterial suspension of Priesteria axeterii ZZRQ1, the mass ratio of the volume of the Priesteria axeterii ZZRQ1 bacterial suspension described in the present invention to the modified zeolite is preferably 1-5 mL: 0.5-2 g, more preferably 5 mL: 2 g; the OD of the Priesteria axeterii ZZRQ1 bacterial suspension is 600 The value is preferably 0.185 to 0.733, more preferably 0.733.

[0036] The modified zeolite of the present invention preferably includes acid-modified zeolite or metal-modified zeolite, and more preferably acid-modified zeolite. The preparation method of the acid-modified zeolite of the present invention preferably includes: mixing the zeolite with a sulfuric acid solution having a mass concentration of 5%, stirring at 100°C for 2h, washing the obtained modified zeolite to neutrality, and obtaining the acid-modified zeolite. The mass ratio of the zeolite of the present invention to the sulfuric acid solution is preferably 1g:4mL. The present invention preferably uses deionized water for the washing. The present invention does not specifically limit the stirring method, and the conventional washing method in the art can be used. The preparation method of the metal-modified zeolite of the present invention preferably includes: mixing zeolite, ferrous sulfate and water, stirring at 30°C for 2h, and drying the obtained modified zeolite to obtain the metal-modified zeolite. The mass ratio of the zeolite and ferrous sulfate of the present invention is preferably 20:1; the mass ratio of the zeolite to water is preferably 125g:500mL. The stirring speed of the present invention is preferably 150r / min. The present invention has no particular limitation on the drying method, and any conventional drying method in the art may be used.

[0037] The present invention also provides a method for preparing the bacterial agent of the above technical solution, comprising the following steps: mixing a bacterial suspension of Priesteria axyri ZZRQ1 with a modified zeolite and adsorbing the mixture under oscillation conditions for 24 to 36 hours to obtain the bacterial agent. The OD value of the bacterial suspension of Priesteria axyri ZZRQ1 of the present invention is 600 The value is preferably 0.185 to 0.733, more preferably 0.733. The oscillation temperature of the present invention is preferably 20 to 40°C, more preferably 30°C; the rotation speed is preferably 150r / min; the pH value is preferably 7.0; and the dissolved oxygen is preferably 5.0mg / L. The adsorption time of the present invention is preferably 24h.

[0038] The present invention also provides the use of the Apricotella ZZRQ1 described in the above technical solution or the bacterial agent described in the above technical solution or the bacterial agent prepared by the preparation method described in the above technical solution in the treatment of nitrogen-containing wastewater. + The concentration of -N) is preferably 100 to 200 mg / L.

[0039] The present invention also provides a method for treating nitrogen-containing wastewater, comprising any one of the following two items:

[0040] 1) mixing the bacterial agent described in the above technical solution or the bacterial agent prepared by the preparation method described in the above technical solution with nitrogen-containing wastewater and treating the mixture for 6 to 27 hours;

[0041] 2) The bacterial suspension of Priesteria axotris ZZRQ1, modified zeolite and nitrogen-containing wastewater are mixed and treated for 6 to 27 hours; the deposit number of Priesteria axotris ZZRQ1 is CGMCC No.27544.

[0042] In the present invention, the mass of the modified zeolite in the bacterial agent is preferably 1-5 g: 50-200 mL, more preferably 1 g: 50 mL. In the present invention, the OD of the bacterial suspension of Priesteria axyri ZZRQ1 is 600 The value is preferably 0.185-0.733; the volume ratio of the bacterial suspension of Apricot Klebsiella ZZRQ1 to the nitrogen-containing wastewater is preferably 1:20.

[0043] In order to further illustrate the present invention, the technical effects provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0044] Example 1

[0045] 1. Experimental Materials

[0046] 1.1 Isolation, screening and identification of experimental strains

[0047] 1.1.1 Enrichment and domestication

[0048] The riverbed mud was taken from a river in Qiuai Town, Yinzhou District, Ningbo City. After the riverbed mud was taken back to the laboratory, it was promptly placed in the refrigerator and properly shaken before use. 10mL of the mud-water mixture was taken with a pipette and added to 90mL of normal saline. It was placed in a shaker for 8 hours and then taken out. It was allowed to stand for 10 minutes, and then 2mL of the supernatant was taken and inoculated into a 250mL conical flask containing 98mL of sterile LB culture medium. The bottle mouth was sealed with a sealing film and placed in a constant temperature shaker at 30°C and 150r / min for cultivation until obvious turbidity appeared. Then 2mL of LB bacterial solution was taken and added to 98mL of heterotrophic nitrification culture medium. After obvious turbidity appeared, it was transferred to fresh sterile heterotrophic nitrification culture medium, and so on. While transferring, the concentration of ammonium sulfate in the culture medium was increased successively until the content reached 3.0g / L, and then plate separation was performed;

[0049] The components of each 1L heterotrophic nitrification medium are: Na2HPO4·7H2O 7.9g, KH2PO4 1.5g, MgSO4·7H2O 0.1g, C4H4Na2O4·6H2O 10.806g, (NH4)2SO4 0.943g, and trace element solution 2mL / L.

[0050] 1.1.2 Separation

[0051] Take the culture solution that has been domesticated under the condition of ammonium sulfate content of 3.0g / L, and dilute it with sterile water in a gradient manner (10 -1 ~10 -8 ), then take 100 μL of the gradient diluted bacterial solution and evenly spread it on a solid heterotrophic nitrification medium plate with an ammonia nitrogen content of 200 mg / L, mark them with numbers, and culture them in a 30°C biochemical incubator for 2 to 3 days. After the colonies grow, pick colonies with different morphologies and separate them by streaking on the plate. In order to ensure the purity of the strain, repeat streaking two to three times. After the purification work is completed, a single colony is inoculated on an LB slant and stored at 4°C.

[0052] 1.1.3 Screening of dominant strains and preliminary determination of ammonia nitrogen degradation ability

[0053] The purified colonies were picked from the plates and inoculated into 100 mL of sterilized LB liquid medium, and cultured in a shaker at 30 °C and 150 r / min for 10 h. Then, 2 mL of the bacterial solution was inoculated into 98 mL of heterotrophic nitrification medium (Na2HPO4·7H2O 7.9 g, KH2PO4 1.5 g, MgSO4·7H2O 0.1 g, C4H4Na2O4·6H2O 10.806 g, (NH4)2SO4 0.943 g). g , trace element solution 2mL / L), placed in a shaker at 30℃, 150r / min for 24h, then sampled to detect ammonia nitrogen concentration, and compared the degradation ability of each purified strain. All 6 strains had a certain ammonia nitrogen degradation effect, among which ZZRQ1 strain had the best degradation effect, with a 24h ammonia nitrogen degradation rate of 56.3%. Therefore, ZZRQ1 strain was taken as the object of further study.

[0054] Heterotrophic nitrifying bacteria ZZRQ1, through 16S rRNA sequence determination and homology comparison, it was found that the similarity between the strain ZZRQ1 and Priesteria afroides was 99.86%, and then its biological classification was confirmed as Priesteria afroides ZZRQ1, and biological preservation was carried out, and the preservation number was CGMCC No.27544. It has efficient heterotrophic nitrification performance and can effectively remove NH4 in natural water bodies + -N (in subsequent experiments, ZZRQ1 is represented by Q1).

[0055] 1.2 Culture medium and simulated wastewater

[0056] (1) Growth medium of strain Q1: 5 g yeast powder, 10 g tryptone, 10 g NaCl, 1000 mL distilled water, pH 7.2-7.3.

[0057] (2) Activation medium composition: glucose 5 g / L, (NH4)2SO4 1 g / L, K2HPO4 0.5 g / L, MgSO4.7H2O 0.03 g / L, MnSO 4. 4H2O 0.01g / L, FeSO 4. 7H2O 0.01g / L, sterilization at 121℃.

[0058] (3) Simulated wastewater: Na2HPO4·7H2O 7.9 g / L, KH2PO4 1.5 g / L, MgSO4·7H2O 0.1 g / L, C4H4Na2O4·6H2O 10.806 g / L, (NH4)2SO4 0.943 g / L, trace element solution 2 mL / L, pH 7.0-7.5, sterilization at 121 °C.

[0059] 2 Experimental methods

[0060] 2.1 Analysis items and methods

[0061] According to the "Water and Wastewater Monitoring and Analysis Methods", ammonia nitrogen (NH4 + -N): Nessler's reagent spectrophotometry; Number of microorganisms in bacterial suspension: dilution plate count method; OD 600 : Turbidimetric method.

[0062] 2.2 Modification of zeolite

[0063] (1) Acid modification: 50 g of zeolite was dried at 120° C. for 4 h, cooled to room temperature, and then 200 mL of 5% sulfuric acid solution was added. The mixture was stirred for 2 h in a water bath at 100° C., filtered, washed with deionized water until neutral, and dried at 105° C.

[0064] (2) Metal ion modification: The dried zeolite and ferrous sulfate FeSO4 were added into 500 mL of deionized water at a mass ratio of 20:1 (the mass ratio of zeolite to water is 125 g:500 mL), stirred at 150 r / min for 2 h at 30 °C, filtered, and dried for 2 h to obtain the modified material.

[0065] 2.3 Growth curve determination of strain Q1

[0066] Preparation method of bacterial solution: Use an inoculation loop to pick strain Q1 and inoculate it into the growth medium. After shaking culture for 24 hours, wash it three times by centrifugation with sterile water, and finally make bacterial solution with sterile water for standby use. The effective live bacteria concentration of the bacterial solution is 1×10 8 CFU / mL.

[0067] The bacterial solution prepared above was mixed with the sterilized nitrifying bacteria activation medium, and three treatments were set up according to different inoculation volume ratios, and repeated three times: ① Treatment 1 (volume ratio 1:5); ② Treatment 2 (volume ratio 1:10); ② Treatment 3 (volume ratio 1:15). The experimental groups were all kept at 28°C and 130 r.min. -1 Under the same conditions, the culture was shaken in a constant temperature shaking incubator, and samples were taken every 12 hours to measure the OD 600 value, determine the optimal inoculation ratio, and measure continuously for 4 days.

[0068] 2.4 Immobilization of strain Q1 on modified zeolite

[0069] Further select modified materials with good adsorption performance, take 300mL of bacterial liquid cultured on a shaker for 24h, centrifuge at 3000rpm for 20min, wash three times with sterile water, dilute to 50mL with sterile water, add 2g of modified zeolite, adjust the pH to 7.0, and oscillate and adsorb at 150r / min in a 30℃ constant temperature water bath shaker, and control the dissolved oxygen to 5.0mg / L. After the modified material particles are completely settled, absorb the supernatant and dilute it, inoculate it to a plate for constant temperature culture, count the colonies on the plate after 6h, 12h, 24h, and 36h as C1, and the initial bacterial suspension colony count as C0. According to the formula Q1=(C0-C1) / C0×100%, calculate the bacterial immobilization rate.

[0070] 2.5 Effect of microorganisms combined with modified zeolite on ammonia nitrogen removal

[0071] In order to explore the effect of microorganisms combined with modified zeolite on the removal of ammonia nitrogen, five treatments were set up, each with three replicates. 2+ ) modification is represented by Fe-ze, and the strain is represented by Q1. The experimental settings are shown in Table 1. 2g of zeolite before and after modification and 5mL of bacterial solution were added to treat 100mL of simulated wastewater, and samples were taken at 3h, 6h, 9h, 15h, 21h, 27h, and 40h. The adsorption effect of the strain combined with the modified zeolite on ammonia nitrogen was calculated, and the NH4 + -N removal rate, the test results are as follows Figure 6 And as shown in Table 3.

[0072] Q=(C-C0) / C×100%

[0073] Where: Q is NH4 + -N removal rate, %; C is NH4 in CK + -N concentration, mg.L -1 ; C0 is the treated NH4 + -N concentration, mg.L -1 .

[0074] Table 1 Experimental design table

[0075] deal with Grouping Bacterial solution dosage (mL) Material quantity (g) Simulated wastewater (ml) Process 1 CK / / 100 Process 2 Q1 5 / 100 Process 3 Q1+ze 5 2 100 Process 4 Q1+H-ze 5 2 100 Process 5 Q1+Fe-ze 5 2 100

[0076] 3 Results and discussion

[0077] 3.1 Characterization of zeolite properties before and after modification

[0078] The materials before and after the modification were scanned by electron microscope SEM, and the results were as follows: Figures 1 to 3 As shown. The surface of the zeolite before modification is loaded with many impurities ( Figure 1 ), after modification with concentrated sulfuric acid, the purity was significantly improved ( Figure 2 After being modified by FeSO4, a layer of colloid is attached to the surface of the zeolite, which increases the roughness of the zeolite surface ( Figure 3 ).

[0079] The specific surface area, pore volume and pore size of the materials before and after modification were tested and analyzed, and the structural parameters are shown in Table 2. As can be seen from Table 2, the modified materials were treated with concentrated sulfuric acid, Fe 2+ The improved performance of the method greatly increased the specific surface area and pore volume of the zeolite, and the pore size was reduced. Compared with the untreated zeolite, the zeolite 2+ The specific surface area and pore volume of the zeolite modified by the method increased by 474%, 463% and 62.9%, 67.8%, respectively, and the pore diameter decreased by 71% and 74.8%, respectively.

[0080] Table 2 Effects of different modification methods on the specific surface area and porosity of zeolite

[0081] project As is Concentrated sulfuric acid modification Metal ion modification <![CDATA[Specific surface area (m 2 / g)]]> 14.984 86.037 84.417 <![CDATA[Pore volume (cm 3 / g)]]> 0.062 0.101 0.104 Pore ​​size (nm) 17.460 5.018 4.397

[0082] Different modification methods have different mechanisms for improving the adsorption performance of the carrier. Concentrated sulfuric acid modification: remove impurities on the surface of the carrier through the etching effect of strong acid, improve purity, increase pore volume and specific surface area, improve void structure, and thus improve its adsorption performance. 2+ Modification: Fe 2+ It is easy to hydrolyze in aqueous solution to form colloidal substances, which are then evenly loaded on the surface of the carrier, changing its surface properties and increasing its surface roughness, which will be conducive to the occurrence of adsorption reactions.

[0083] 3.2 Determination of the growth curve of strain Q1

[0084] The growth curves of strains with different inoculation volume ratios are shown in Figure 2. Figure 4 As shown. Within 0 to 36 hours, the OD of each treatment 600 The values ​​increased significantly, and the strain entered the logarithmic growth phase. 600 The increasing rate of the values ​​was significantly higher than that of treatment 3. After 36 h, the OD values ​​of each treatment 600The values ​​showed a slow growth trend. At 72h, the OD 600 The values ​​were at the highest values. Then they showed a downward trend. When the inoculation ratio was 1:15, the total amount of bacteria was low due to the small inoculation amount and the low bacterial reproduction base. When the inoculation ratio was 1:5, there were too many bacteria, resulting in a relative lack of nutrients. According to the growth curve of the strain, the optimal inoculation ratio was 1:10.

[0085] 3.3 Immobilization of strain Q1 on modified zeolite

[0086] The effect of adsorption time on the material loading strain Q1 was investigated. Figure 5 As shown. As can be seen from the figure, with the increase of adsorption time, the immobilization rate of the material for strain Q1 shows a trend of first rising and then falling. In the initial stage, the adsorption process is fast. After 12 hours, the adsorption efficiency slowly decreases. After 24 hours of adsorption, the immobilization rate reaches the maximum, among which the immobilization rates of concentrated sulfuric acid and FeSO4 modified reach 86.1% and 69.1% respectively. As time continues to increase, the immobilization rate begins to decrease. Due to the lack of nutrients, the strain cannot grow and reproduce, and the adhesion between bacteria decreases. Under the action of external forces, strain Q1 may gradually fall off from the surface of the material, so the optimal adsorption time is determined to be 24 hours.

[0087] 3.4 Effect of bacterial strain combined with modified zeolite on ammonia nitrogen removal

[0088] Table 3 Effect of microbial combined carrier on NH4 + -N removes the effect

[0089] deal with 3h 6h 9h 15h 21h 27h Q1 6.4±3.4b 23.8±2.8b 34.0±2.5b 60.9±0.7b 78.1±2.2b 96.5±0.2c ze+Q1 21.7±1.9a 34.0±2.9a 39.6±1.4b 64.9±1.7ab 83.6±3.2ab 98.7±0.2b H-ze+Q1 23.3±2.4a 38.3±0.4a 49.3±2.3a 68.0±2.5a 88.4±0.9a 100.0±0.1a Fe-ze+Q1 23.5±3.6a 34.7±0.3a 40.1±2.4b 65.3±1.8ab 89.1±0.3a 100.0±0.1a

[0090] Note: Different letters in Table 3 indicate P < 0.05.

[0091] Figure 6 Table 3 shows the effects of microbial combined carriers on NH4 + -N removes the effect, Figure 6 Q1 in the table corresponds to Q1 in Table 3. The test was carried out for 3 hours, which is the initial stage. The microorganisms have not yet adapted to the new environment and mainly adsorb on the carrier. The microorganisms + The removal rate of -N was only 6.4%, and the removal rate of zeolite and modified zeolite for NH4 + -N removal rate is 21.7% to 23.5%. As the reaction time increases, the microorganisms begin to adapt to the new environment. After 6h to 27h, the removal of NH4 + -N removal rate is 23.8% to 96.5%, zeolite combined with bacteria to NH4 + -N removal rate is 34.0% to 98.7%, acid-modified zeolite combined with bacterial agent for NH4 +-N removal rate is 38.3%~100.0%, iron-modified zeolite combined with bacterial agent NH4 + -N removal rate is 34.7% to 100.0%. However, the removal of NH4 + -N removal rates were higher than those of free bacteria. Among them, the zeolite modified with concentrated sulfuric acid and metal ions combined with microorganisms had a higher removal rate of NH4 + -N removal rate is higher than that of zeolite combined with microorganisms. On the one hand, because zeolite itself has a certain adsorption effect, the modified zeolite increases the pore volume and specific surface area, improves the pore structure, and thus improves its adsorption performance for sewage and the solidification rate of bacteria. Microorganisms are loaded on it and continue to play a good role in NH4 + -N removes the effect.

[0092] Example 2

[0093] 2.1 Experimental Materials

[0094] Domestic sewage was collected from a pond inside a community in Qiuai Town, Yinzhou District, Ningbo City, Zhejiang Province.

[0095] 2.2 Experimental equipment

[0096] The experiment used a laboratory-scale aerated bioreactor, such as Figure 7 As shown, a biofilter tank with the bacteric acid-modified zeolite (Q1+H-Ze) prepared in Example 1 as filler was constructed. The bacteric acid-modified zeolite was placed on the perforated plate, an aeration head was set on one side of the bottom, the dissolved oxygen content (DO) was controlled at about 5.0 mg / L, and the temperature was controlled at 25°C. Continuous water inflow was adopted, and aeration was performed from the bottom of the reactor using an air pump, water was introduced into the other side of the bottom, and water was discharged from the top.

[0097] 2.3 Analysis methods

[0098] In this experiment, the reactor inlet and outlet water were sampled every day. According to the "Water and Wastewater Monitoring and Analysis Methods", the test indicators were ammonia nitrogen (NH4 + -N): Nessler's reagent spectrophotometry. Take influent and effluent samples after 3h, 12h, 24h, 36h, 48h, 72h, and 96h, centrifuge and determine the NH4 in the solution + -N concentration, and calculate NH4 using the following formula + -N removal rate, the test results are as follows Figure 8 And as shown in Table 4.

[0099] q=(C0-C1) / C0×100%

[0100] Where: q is NH4 + -N removal rate, %; C0 is the NH4 influent in the corresponding days + -N concentration, mg.L -1; C1 is the effluent NH4 after treatment + -N concentration, mg.L -1 .

[0101] Table 4 Treatment effect of acid-modified zeolite loaded with strain Q1 on domestic sewage

[0102] Ammonia nitrogen concentration (mg / L) 3h 12h 24h 36h 48h 72h 96h Water Intake 13.91 14.50 15.29 14.22 14.57 18.42 17.57 Water 9.16 7.30 6.87 5.82 5.14 4.76 4.67 Ammonia nitrogen removal rate % 34.15 49.66 55.06 59.06 64.68 74.18 73.43

[0103] Depend on Figure 8 As shown in Table 4, the bacteria-modified zeolite has a good effect on domestic sewage treatment. After 96 hours of operation, NH 4+ -N concentration increased from the initial inlet concentration of 13.91 mg / L and the outlet concentration of 9.16 mg / L to the inlet concentration of 17.57 mg / L and the outlet concentration of 4.67 mg / L. 4+ -N removal rate increased from the initial 34.15% to 73.43%. The removal rate showed an upward trend with the increase of time. The removal rate reached the highest at 72h, which was 74.18%. Then the pollutant concentration remained basically stable and the adsorption reached equilibrium. The experiment proved that the use of bacteria-modified zeolite in the reactor had a good continuous treatment effect on the removal of ammonia nitrogen from domestic sewage.

[0104] It can be concluded from the above examples that the present invention combines the degradation performance of the heterotrophic nitrification-aerobic denitrification bacteria Priesteria africana ZZRQ1 with the adsorption performance of the modified zeolite to better remove ammonia nitrogen in nitrogen-containing wastewater.

[0105] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A Priestia aryabhattai ZZRQ1, characterized in that The deposit number of the Priesteria afroides ZZRQ1 is CGMCC No.27544.

2. A bacterial agent containing Priesteria afroicola ZZRQ1 according to claim 1, characterized in that: The bacterial agent comprises Priesteria afroyi ZZRQ1 and modified zeolite, and the Priesteria afroyi ZZRQ1 is loaded on the surface of the modified zeolite.

3. The bacterial agent according to claim 2, characterized in that Calculated based on the bacterial suspension of Priesteria axotris ZZRQ1, the mass ratio of the volume of the bacterial suspension of Priesteria axotris ZZRQ1 to the modified zeolite is 1 to 5 mL: 0.5-2 g, the OD of the bacterial suspension of A. aegypti ZZRQ1 600 The values ​​are 0.185~0.

733.

4. The bacterial agent according to claim 2 or 3, characterized in that: The modified zeolite includes acid-modified zeolite or metal-modified zeolite; The preparation method of the acid modified zeolite comprises: mixing the zeolite with a sulfuric acid solution having a mass concentration of 5%, stirring at 100° C. for 2 hours, washing the obtained modified zeolite to neutrality, and obtaining the acid modified zeolite; The preparation method of the metal-modified zeolite comprises: mixing zeolite, ferrous sulfate and water, stirring at 30° C. for 2 hours, and drying the obtained modified zeolite to obtain the metal-modified zeolite.

5. The bacterial agent according to claim 4, characterized in that During the preparation of the acidic modified zeolite, the volume ratio of the zeolite mass to the sulfuric acid solution is 1 g:4 mL.

6. The bacterial agent according to claim 4, characterized in that In the preparation process of the metal-modified zeolite, the mass ratio of the zeolite to ferrous sulfate is 20:1, and the volume ratio of the mass of the zeolite to water is 125 g:500 mL.

7. The method for preparing the bacterial agent according to any one of claims 2 to 6, characterized in that: The method comprises the following steps: mixing a bacterial suspension of Priesteria axyri ZZRQ1 with modified zeolite, and then adsorbing the mixture for 24 to 36 hours under oscillation conditions to obtain the bacterial agent.

8. Use of the Priesteria axytogenes ZZRQ1 described in claim 1, the bacterial agent described in any one of claims 2 to 6, or the bacterial agent prepared by the preparation method described in claim 7 in the treatment of nitrogen-containing wastewater.

9. A method for treating nitrogen-containing wastewater, characterized in that: Includes any one of the following: 1) mixing the bacterial agent described in any one of claims 2 to 6 or the bacterial agent prepared by the preparation method described in claim 7 with nitrogen-containing wastewater and treating the mixture for 6 to 27 hours; 2) The bacterial suspension of Priesteria axotris ZZRQ1, modified zeolite and nitrogen-containing wastewater are mixed and treated for 6 to 27 hours; the deposit number of Priesteria axotris ZZRQ1 is CGMCC No.27544.

10. The method according to claim 9, characterized in that In the above 1), based on the mass of the modified zeolite in the bacterial agent, the volume ratio of the mass of the modified zeolite to the nitrogen-containing sewage is 1-5 g: 50-200 mL; In the above 2), the OD value of the bacterial suspension of Priesteria axyri ZZRQ1 is 600 The value is 0.185-0.733; the volume ratio of the bacterial suspension of Apricot Klebsiella ZZRQ1 to the nitrogen-containing sewage is 1:20.

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