A microbial composite carrier material for industrial sewage treatment and its preparation method and application

Abstract

The present invention relates to the technical field of wastewater treatment, in particular to a microbial composite carrier material for industrial wastewater treatment, and a preparation method and application thereof, wherein the material is a biological inorganic material formed by a composite microbial strain and an immobilized carrier material; wherein the composite microbial strain is prepared by mixing bacterial liquids of Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus omnivorus, and Bacillus subtilis; and the carrier material is expanded perlite, foamed cement or porous ceramic, which is used to improve and strengthen the removal capacity of microorganisms for COD and total nitrogen in industrial wastewater. The microbial composite carrier material can significantly improve the removal rate of COD and ammonia nitrogen in industrial wastewater, improve the effluent water quality, promote standard discharge, and does not generate new environmental pollution during use, and has good application value.

Description

Technical Field

[0001] The present invention relates to the technical field of wastewater treatment, and specifically relates to a microbial composite carrier material for industrial wastewater treatment, a preparation method and an application thereof. Background Art

[0002] At present, most industrial wastewater in my country is treated by biological methods. Biological treatment methods have the advantages of good treatment effect, low investment and operation cost, etc., mainly including activated sludge method and biofilm method. The activated sludge method uses suspended microbial populations and sewage aeration to mix and cultivate, and has developed a variety of mature operation processes. In the existing biofilm method, microorganisms grow on the surface of the carrier in an attached form, which improves the reactor volume load and organic matter removal efficiency. Microbial composite carriers are key materials for the biofilm process. Existing carrier materials generally have problems such as easy detachment of biofilms, clogging and agglomeration, low mass transfer rate or small specific surface area. Therefore, the development of new green and efficient biological carriers is of great significance to improving the treatment efficiency of biological contact oxidation processes.

[0003] In addition, in the biofilm process, depending on the different environments in which microorganisms grow on the material, an "anaerobic layer" and an "aerobic layer" will be formed on the surface of the material. However, the current microbial carrier materials are often solid structures with small specific surface areas, small growth of microorganisms attached to the material, and no good "anaerobic layer" is formed. Therefore, the removal efficiency of ammonia nitrogen and total nitrogen is not high, and the overall removal efficiency of pollutants in sewage per unit volume of material is not high.

[0004] Therefore, there is a need in the art to develop a new microbial composite carrier material to improve the material's ability and effect in removing pollutants. Summary of the invention

[0005] The purpose of the present invention is to provide a microbial composite carrier material for industrial wastewater treatment, a preparation method and application thereof. The microbial composite carrier material has stable properties and can efficiently remove COD, ammonia nitrogen, total nitrogen and total phosphorus in industrial wastewater, is easy to use and has good effects.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A microbial composite carrier material for industrial sewage treatment is a biological inorganic material formed by a composite microbial strain and an immobilized carrier material; wherein the composite microbial strain is prepared by mixing bacterial liquids of Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus holophaga and Bacillus subtilis.

[0008] The composite microbial strain is prepared by mixing bacterial liquids of Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus holophaga and Bacillus subtilis in a volume ratio of 6:3:5:2:2.

[0009] The above strains of the present invention can be purchased from strain collection centers such as the China General Microbiology Center (CGMCC), the China Center for Type Culture Collection (CCTCC) and the American Type Culture Collection (ATCC).

[0010] Preferably,

[0011] The Pseudomonas stutzeri is Pseudomonas stutzeri CGMCC 1.10279;

[0012] The Pseudomonas aeruginosa is Pseudomonas aeruginosa CGMCC 1.10274;

[0013] The alcaligenes faecalis is Alcaligenes faecalis CGMCC 1.924;

[0014] The Paracoccus pantotrophus CGMCC 1.8851;

[0015] The Bacillus subtilis CGMCC 1.9083.

[0016] Wherein, the carrier material is expanded perlite, foamed cement or porous ceramic. Preferably, the carrier material is modified porous ceramic, and its pore size is 0.2-5mm, wherein 0.5-2mm is preferred, and 1-1.5mm is the most preferred. The experimental results show that the inorganic material using modified porous ceramic as a carrier can form a symbiotic relationship with microorganisms to form a new bio-inorganic material.

[0017] Wherein, the surface modifier used in the modified porous ceramic is at least one of cationic waterborne polyurethane, epoxy resin, cationic surfactant, and silane coupling agent, among which silane coupling agent is the best, followed by cationic waterborne polyurethane. Preferably, the surface modifier is a silane coupling agent, including amino-substituted siloxane, vinyl-substituted siloxane, alkyl-substituted siloxane, and carboxyl-substituted siloxane, among which amino-substituted siloxane is the best.

[0018] The method for preparing the microbial composite carrier material of the present invention comprises the following steps:

[0019] (1) Preparation of composite microbial strains: Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus omnivorus, and Bacillus subtilis were cultured to a concentration of 1×10 8 ~1×10 9 The bacterial solution was prepared by mixing the mixture in a volume ratio of 6:3:5:2:2.

[0020] The preparation steps are as follows: inoculating each strain into a culture medium respectively, expanding and culturing at 15-35°C for 48-96 hours, and mixing the obtained microbial fermentation broths according to a ratio to obtain the microbial agent composite microbial strain; wherein the components of the culture medium are 6-10 g / L glucose, 2-2.2 g / L urea, 0.8-1.0 g / L potassium dihydrogen phosphate, 0.1-0.2 g / L magnesium sulfate, and 8-12 g / L sodium chloride.

[0021] (2) Preparation of immobilized carrier material:

[0022] ① Take a porous ceramic with a pore size of about 1-2mm and clean its surface with alcohol or acetone;

[0023] ② Immerse the porous ceramic in clean water, add amino-substituted siloxane and stir;

[0024] ③ Dry the surface-modified porous ceramic in an oven at 100-120°C and then cool it to obtain an immobilized carrier material;

[0025] (3) The composite microbial strains and the immobilized carrier material are cultured together in a culture medium for 6 to 7 days to obtain the product.

[0026] The microbial composite carrier material prepared by the present invention can be directly used for industrial wastewater treatment. The amount of the microbial composite carrier material is 0.1-0.2% of the volume of the wastewater, and the removal rate of COD within 24 hours can reach 95-96%, the removal rate of ammonia nitrogen can reach 90-91%, the removal rate of total nitrogen is stable at 80-85%, and the removal rate of total phosphorus is 75-77%.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] In the microbial composite carrier material prepared by the present invention, the amount of microorganisms attached to the carrier per unit volume is relatively large, reaching 20-25%, and the microorganisms attached and grown on the surface of the material not only have conventional "aerobic layers" but also more "anaerobic layers". The material has a developed pore structure and has significant adsorption and removal effects on pollutants in wastewater. At the same time, the combined action of the "anaerobic layer" and "aerobic layer" microorganisms on the surface of the material can effectively remove COD, ammonia nitrogen and total nitrogen. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Example 1 Preparation of composite microbial strains in microbial agents

[0031] Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus omnivorus, and Bacillus subtilis were cultured to a concentration of 1×10 8 ~1×10 9 / mL of bacterial solution, and then mix them in a volume ratio of 6:3:5:2:2.

[0032] The above strains were purchased from the General Microbiology Center of China Microorganism Culture Collection (CGMCC), China Center for Type Culture Collection (CCTCC) and American Type Culture Collection (ATCC). Pseudomonas stutzeri was Pseudomonas stutzeri CGMCC 1.10279; Pseudomonas aeruginosa was Pseudomonas aeruginosa CGMCC 1.10274; Alcaligenes faecalis was Alcaligenes faecalis CGMCC 1.924; Paracoccus pantotrophus CGMCC1.8851; Bacillus subtilis CGMCC 1.9083.

[0033] The specific preparation steps are: inoculate each strain into the culture medium respectively, expand the culture at 35°C for 48 hours, and mix the obtained microbial fermentation broth according to a ratio to obtain the main material of the microbial agent; wherein the components of the culture medium are 10 g / L glucose, 2.0 g / L urea, 1.0 g / L potassium dihydrogen phosphate, 0.2 g / L magnesium sulfate, and 8 g / L sodium chloride.

[0034] In order to highlight the beneficial effects of the present invention, the following comparative example experiments are given.

[0035] Comparative Example 1-3 Bacterial Liquid Preparation

[0036] The bacterial solution was prepared in the same manner as in Example 1, except that the ratio of different bacterial species was changed to obtain the following bacterial solution formula, which is summarized as follows:

[0037] Table 1 Bacterial liquid formula ingredients

[0038]

[0039] Example 2 Preparation of microbial composite immobilization carrier material

[0040] 20g of porous ceramic with an average pore size of 2-3mm was immersed in 100mL of anhydrous ethanol for 30min, then washed with clean water, immersed in a solution containing 1% aminopropyltrimethoxysilane for 30min, and then taken out. After washing with clean water, it was dried in an oven at 100-120°C to obtain a carrier material.

[0041] The carrier material is added to the culture medium containing the composite bacterial solution in Example 1 and cultured for 6 to 7 days to obtain a microbial composite carrier material.

[0042] Example 3-8 Preparation of Microbial Composite Immobilization Carrier Material

[0043] Different composite carrier materials were prepared according to the scheme of Example 2, and only the pore size of the porous ceramic, the silane surface modifier and the bacterial solution were changed. The following table is organized:

[0044] Table 2 Microbial composite carrier materials

[0045] serial number Ceramic pore size Silane coupling agent Compound bacterial liquid Composite Materials Example 2 2-3mm Aminopropyltrimethoxysilane I A Example 3 1-2mm Aminopropyltrimethoxysilane I B Example 4 1-2mm Allyltrimethoxysilane I C Example 5 1-2mm n-Butyltrimethoxysilane I D Example 6 1-2mm Aminopropyltrimethoxysilane II E Example 7 1-2mm Aminopropyltrimethoxysilane III F Example 8 1-2mm Aminopropyltrimethoxysilane IV G

[0046] Application example: Composite microbial carrier materials for treating industrial wastewater

[0047] The industrial wastewater in this embodiment comes from the aerobic aeration section of a sewage treatment plant. 2000 mL of muddy water is taken and the supernatant is taken after natural sedimentation. The content of major pollutants in the supernatant is detected as the experimental object.

[0048] Table 3 Treatment effect of microbial composite carrier materials on industrial wastewater

[0049]

[0050]

[0051] It can be seen that the microbial composite material B prepared by modifying the surface of the porous ceramic with an average pore size of 1-2 mm in Example 3 can effectively remove pollutants, with a COD removal rate of more than 95%, ammonia nitrogen between 88% and 90%, a total nitrogen removal rate of about 80% to 85%, and a total phosphorus removal rate of 75% to 77%. With reference to relevant national environmental protection regulations, the above indicators of the treated sewage all meet the requirements of the "Comprehensive Sewage Discharge Standard of the People's Republic of China" (GB8978-2002).

[0052] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microbial composite carrier material for industrial wastewater treatment, characterized in that: The bio-inorganic material is formed by a composite microbial strain and an immobilized carrier material; wherein the composite microbial strain is prepared by mixing bacterial liquids of Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus omnivorus, and Bacillus subtilis; and the carrier material is expanded perlite, foamed cement or porous ceramics.

2. The microbial composite carrier material for industrial wastewater treatment according to claim 1, characterized in that: in, The composite microbial strain is prepared by mixing bacterial liquids of Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus holophaga and Bacillus subtilis in a volume ratio of 6:3:5:2:

2.

3. The microbial composite carrier material for industrial wastewater treatment according to claim 1, characterized in that: The Pseudomonas stutzeri is Pseudomonas stutzeri CGMCC 1.10279; The Pseudomonas aeruginosa is Pseudomonas aeruginosa CGMCC 1.10274; The alcaligenes faecalis is Alcaligenes faecalis CGMCC 1.924; The Paracoccus pantotrophus CGMCC 1.8851; The Bacillus subtilis CGMCC 1.9083.

4. The microbial composite carrier material for industrial wastewater treatment according to claim 1, characterized in that: The carrier material is modified porous ceramic, and the pore size thereof is 0.2-5 mm.

5. The microbial composite carrier material for industrial wastewater treatment according to claim 4, characterized in that: The surface modifier used in the modified porous ceramic is at least one of cationic waterborne polyurethane, epoxy resin, cationic surfactant and silane coupling agent.

6. The microbial composite carrier material for industrial wastewater treatment according to claim 5, characterized in that: The surface modifier is a silane coupling agent, including amino-substituted siloxane, vinyl-substituted siloxane, alkyl-substituted siloxane and carboxyl-substituted siloxane.

7. The microbial composite carrier material for industrial wastewater treatment according to claim 6, characterized in that: The surface modifier is amino-substituted siloxane.

8. The method for preparing a microbial composite carrier material for industrial wastewater treatment according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) Preparation of composite microbial strains: Pseudomonas stutzeri, Pseudomonas aeruginosa, Alcaligenes faecalis, Paracoccus omnivorus, and Bacillus subtilis were cultured to a concentration of 1×10 8 ~1×10 9 The bacterial solution was prepared by mixing the mixture in a volume ratio of 6:3:5:2:

2. (2) Preparation of immobilized carrier material: ① Take a porous ceramic with a pore size of about 1-2mm and clean its surface with alcohol or acetone; ② Immerse the porous ceramic in clean water, add amino-substituted siloxane and stir; ③ Dry the surface-modified porous ceramic in an oven at 100-120°C and then cool it to obtain an immobilized carrier material; (3) The composite microbial strains and the immobilized carrier material are cultured together in a culture medium for 6 to 7 days to obtain the product.

9. The method for preparing a microbial composite carrier material for industrial sewage treatment according to claim 8, characterized in that: In the step (1), each bacterial strain is inoculated into a culture medium, expanded and cultured at 15 to 35° C. for 48 to 96 hours, and the obtained microbial fermentation broths are mixed according to a ratio to obtain the microbial agent composite microbial strain; wherein the composition of the culture medium is 6 to 10 g / L glucose, 2 to 2.2 g / L urea, 0.8 to 1.0 g / L potassium dihydrogen phosphate, 0.1 to 0.2 g / L magnesium sulfate, and 8 to 12 g / L sodium chloride.

10. Use of the microbial composite carrier material according to any one of claims 1 to 7 in industrial wastewater treatment, characterized in that: The dosage of the microbial composite carrier material is 0.1 to 0.2% of the sewage volume.

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