Color developing solution and method for rapidly identifying cortex cinnamomi germplasm suitable for preparing cinnamaldehyde fragrant essential oil

Abstract

The invention discloses a developing solution for rapidly identifying a cinnamon germplasm suitable for preparing cinnamic aldehyde fragrant essential oil and a method for rapidly identifying the cinnamon germplasm suitable for preparing cinnamic aldehyde fragrant essential oil. The developing solution comprises a mixed solution prepared from a 40% sulfuric acid methanol solution containing 4-6% of vanillin and a 1 mol / L sodium hydroxide solution according to the volume ratio of 3: 1. The method comprises the following steps: S1, collecting barks of cinnamon plants; s2, preparing a developing solution on site; and S3, adding the color developing solution into the bark, reacting, transferring the color developing solution after the reaction, comparing the color depths of the color developing solution after the reaction, and screening out cinnamon plants with the color depth reaching a certain degree, namely the candidate germplasm suitable for preparing cinnamaldehyde fragrant essential oil. The screening link is easy and convenient to operate, low in cost, rapid and accurate, candidate germplasm can be rapidly and accurately screened out from a large number of germplasm, compared with a direct identification method, the workload is greatly reduced, the identification period is shortened, the identification efficiency is improved, and high operability and wide applicability are achieved.

Description

Technical Field

[0001] The present invention relates to the field of identification and utilization of Chinese medicinal material germplasm and new variety breeding. More specifically, the present invention relates to a color developing solution and a method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil. Background Art

[0002] Cinnamon( Cinnamomum cassia Presl) is Lauraceae ( Lauraceae ) Cinnamomum Cinnamomum ) is an evergreen tree, also known as cinnamon, which is mainly produced in Guangxi, Guangdong, Fujian and other regions. Its dried bark (cinnamon bark), young branches (cinnamon twigs), young fruits (cinnamon seeds) and petioles (cinnamon taro) can all be used as medicine. Cinnamon is included in the 2024 edition of the Pharmacopoeia of the People's Republic of China. It is pungent, sweet, and very hot. It enters the kidney, spleen, heart, and liver meridians. It has the effects of replenishing fire and assisting yang, guiding fire back to the origin, dispersing cold and relieving pain, and warming the meridians. It is used for impotence, cold uterus, cold pain in the waist and knees, and asthma caused by kidney deficiency. As a medicinal material that can be used as both medicine and food, cinnamon has a long history of medicinal and edible use. Related products involve the fields of medicine, condiments, daily necessities, food, pesticides, etc., and have broad prospects for development and utilization. Cinnamon contains a variety of chemical components, among which essential oils are the main active substances. The components that have been identified so far include cinnamaldehyde, coumarin, cinnamic acid, etc., among which cinnamaldehyde is the main component of volatile oil, accounting for 75%~90%. Guangxi's cinnamon planting area accounts for more than 50% of the country's cinnamon planting area, and the annual output of cinnamon volatile oil is about 1,000 tons, accounting for more than 50% of the national output. "Chinese cinnamon oil" has a strong spicy aroma, full of rich cinnamon and spicy aroma, and the aroma is long-lasting, enjoying a high reputation internationally.

[0003] Volatile oil content is one of the important indicators for evaluating the quality of cinnamon medicinal materials. The aroma of essential oil is best with the unique strong spicy aroma of cinnamaldehyde. The formation of its cinnamaldehyde aroma is mainly affected by germplasm, ecological environment, cultivation measures and processing technology, among which germplasm is the basis. Since the growth cycle of cinnamon is relatively long (generally more than 7 years), and the identification of excellent cinnamon germplasm is mainly based on the traditional direct identification method, this method requires large-scale peeling and sampling of cinnamon germplasm planted in the field to detect the content of volatile oil and cinnamaldehyde, which is a considerable workload, and has the disadvantages of long identification cycle, complex identification procedures, and a large amount of manpower, material and financial resources. As a result, there is still a lack of high-quality germplasm or varieties with high oil content or high cinnamaldehyde content in production. Summary of the invention

[0004] An object of the present invention is to solve at least the above problems and to provide at least the advantages which will be described hereinafter.

[0005] In order to achieve these purposes and other advantages according to the present invention, a color developing solution is provided for quickly identifying cinnamon germplasm suitable for making cinnamon aldehyde aroma essential oil, the color developing solution comprising a mixture of 40% sulfuric acid methanol solution containing 4-6% vanillin and 1 mol / L sodium hydroxide solution in a volume ratio of 3:1.

[0006] The present invention also provides a method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, comprising the following steps: S1. Collect bark from 7-10 year old cinnamon plants at a height of 1-1.5 m above the ground. The bark size is 1-2 cm 2 of square; S2, preparing a 40% sulfuric acid methanol solution containing 4-6% vanillin, and then mixing it with a 1 mol / L sodium hydroxide solution in a volume ratio of 3:1 to obtain the color developing solution; S3, adding the color developing solution to the collected bark for reaction, transferring the color developing solution after the reaction, comparing the color depth of the color developing solution after the reaction, and screening out cinnamon plants with a color depth reaching a certain degree, which are candidate germplasms suitable for producing cinnamaldehyde-flavored essential oil.

[0007] Preferably, the method further comprises the following steps: S4, detecting the volatile oil content and cinnamaldehyde content of the candidate germplasm screened out in step S3, and calculating the mass ratio of cinnamaldehyde to volatile oil; S5. Select candidate germplasms with volatile oil content ≥ 2% and cinnamaldehyde content as a percentage of volatile oil content ≥ 75%.

[0008] Preferably, in step S3, the color depth reaching a certain degree specifically means that the color depth reaches the color depth of the reaction between the cinnamaldehyde standard of 5 mg / mL and the color developing solution.

[0009] Preferably, the content of cinnamaldehyde is detected by gas chromatography-mass spectrometry, wherein the regression equation of cinnamaldehyde is: Y=2268936277.15310 x-55058931.85130.

[0010] The present invention has at least the following beneficial effects: First, the screening process of the present invention is simple to operate, low-cost, fast and accurate, and can quickly and accurately screen out candidate germplasms from a large number of germplasms. The screening process only takes a few minutes (5 min) and the analysis process only takes 1 working day. Compared with the direct detection and identification method, it greatly reduces the workload, shortens the identification cycle, and improves the identification efficiency. It has strong operability and wide applicability.

[0011] Second, the present invention has established a method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil. This method uses a small amount of representative fresh bark samples (only 1-2 cm in size) of different germplasms. 2 ) as the material, under the action of the independently developed color developing solution, excellent germplasm with high volatile oil and / or cinnamaldehyde content can be quickly screened out.

[0012] Third, the present invention further carries out accurate identification of candidate excellent germplasms, extracts their volatile oils, and uses GC-MS technology to determine their volatile oil content and cinnamaldehyde content. When the volatile oil content is ≥2% and the cinnamaldehyde content accounts for ≥75% of the volatile oil content, the germplasm is determined to be cinnamon germplasm suitable for making cinnamaldehyde-flavored essential oil. This link provides evidence for the dyeing and screening link, and can more accurately determine excellent germplasm.

[0013] Fourth, in the early stage of the present invention, 2000 cinnamon germplasms of seven years old in the main production area of ​​Guangxi were screened, and the results showed that the volatile oil content and cinnamaldehyde content of the candidate excellent germplasms screened out through the dyeing identification link were significantly higher than the general germplasms of the same growth year, and the volatile oil content mostly exceeded 3%, and the cinnamaldehyde content accounted for more than 80% of the volatile oil content. Therefore, the method combining dyeing identification with content determination can quickly and accurately determine the cinnamon germplasm suitable for making cinnamaldehyde-flavored essential oil.

[0014] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is the color diagram of the reaction between different concentrations of cinnamaldehyde standard and a mixed solution of vanillin, methanol, sulfuric acid and sodium hydroxide; Figure 2 This is a color diagram of the reaction between the cinnamon phloem of 100 plants from one of the production areas and a mixed solution of vanillin, methanolic sulfuric acid and sodium hydroxide; Figure 3 This is the total ion chromatogram of the essential oil of the candidate excellent germplasm Ccas-49; Figure 4 This is the peak diagram of cinnamaldehyde reference substance. DETAILED DESCRIPTION

[0016] The present invention is further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0017] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified; in the description of the present invention, the orientation or positional relationship indicated by the terms is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0018] <Staining Screening> Step 1: Use a square of 1 cm 2 Punch holes (tool chisel) were used to chisel holes in the bark (phloem) of cinnamon plants at a height of 1 to 1.5 m above the ground. The holes were 10 mm × 10 mm in shape and 1 cm in area. 2 Square block samples. The punching area can also be selected to be 2 cm 2 The sample area is 1~2 cm 2 That's it.

[0019] Step 2: Place the sample in a 5 mL centrifuge tube, number it, cover it, and develop the color immediately or store it in dry ice and send it to the laboratory for development after thawing. The color development solution configuration specifically includes: ① Slowly add 100 mL of concentrated sulfuric acid into an appropriate amount of methanol (analytical grade), and then dilute to 250 mL with methanol to make a 40% sulfuric acid methanol solution, and cool to room temperature; ② Accurately weigh 12.5 g of vanillin, add it to 250 mL of 40% sulfuric acid methanol solution, and shake it slowly to fully dissolve it to obtain a 40% sulfuric acid methanol solution containing 5% vanillin. This solution must be prepared and used immediately; ③ Prepare a sodium hydroxide solution with a concentration of 1 mol / L, that is, accurately weigh 40 g of sodium hydroxide solid, pour the weighed powder into a beaker, dissolve it with a small amount of distilled water, cool it to room temperature, transfer the cooled solution to a 1 L volumetric flask under the drainage of a glass rod, wash the beaker and glass rod with distilled water 2-3 times, and dilute to 1 L with distilled water; ④ Mix 40% sulfuric acid methanol solution containing 5% vanillin and 1 mol / L sodium hydroxide solution in a ratio of 3:1 to obtain a color developing solution.

[0020] Step 3: Add 2 mL of color developing solution into a centrifuge tube and react for 2 min, then transfer 1 mL of color developing solution to a transparent well plate, compare the color depths of the color developing solutions of various germplasms, and screen out germplasms with a color depth of 5 mg / mL of cinnamaldehyde standard and the color depth of the color developing solution, and preliminarily determine them as candidate germplasms suitable for making cinnamaldehyde essential oil.

[0021] Step 4: Collect the bark of the candidate germplasm and make a dry sample, extract its volatile oil, and determine its volatile oil content and cinnamaldehyde content. When the volatile oil content is ≥2% and the cinnamaldehyde content accounts for ≥75% of the volatile oil content, the germplasm is determined to be cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil.

[0022] in, Figure 1 The color diagram of the reaction of different concentrations of cinnamaldehyde standard with a mixed solution of vanillin, methanol, sulfuric acid and sodium hydroxide. Cinnamaldehyde standard solutions with concentrations of 0.5 mg / mL, 2.5 mg / mL, 5 mg / mL, 10 mg / mL and 15 mg / mL were prepared, 2 mL of color solution was added to a centrifuge tube (with 0.2 mL of cinnamaldehyde standard solution in the centrifuge tube) and reacted for 2 min, 1 mL of color solution was transferred to a transparent well plate, and the color results corresponding to each concentration were obtained, and the color results were saved for later use.

[0023] <Volatile Oil Extraction> Essential oil extraction involves the following steps: Step 1: Select candidate germplasm with darker staining and peel off about 300-500 cm of cinnamon plants at a height of 1-1.5 m above the ground. 2 The bark (phloem) was taken back to the laboratory.

[0024] Step 2: Chop the bark into pieces and place in a 40°C constant temperature oven to dry for 24 hours. After crushing, pass through a 40-mesh sieve and seal for later use.

[0025] Step 3: Accurately weigh 50 g of cinnamon powder into a round-bottom flask with a solid-liquid ratio of 1:8, shake and mix, soak for 1 h, perform steam distillation extraction, collect the obtained cinnamon volatile oil, add an appropriate amount of anhydrous sodium sulfate overnight, and obtain cinnamon essential oil. Each sample was measured three times, and the content was averaged.

[0026] Step 4: dilute the cinnamon essential oil with ethyl acetate to a volume of 12.5 mg / mL. Use gas chromatography-mass spectrometry to determine the content of cinnamaldehyde.

[0027] <Detection of cinnamaldehyde content in volatile oil by gas chromatography-mass spectrometry> The method for detecting the cinnamaldehyde content in volatile oil by gas chromatography-mass spectrometry comprises the following steps: Step 1: The injection volume of the cinnamon volatile oil dilution was 1 μL each time, and this step was repeated 3 times.

[0028] Step 2: Use Trace1300-TSQ8000 gas chromatograph-mass spectrometer for determination. The chromatographic column used is a TG-5SILMS capillary gas chromatograph column (30 m, 0.25 mm, 0.25 μm); the gas phase conditions are as follows: inlet temperature 230°C, split ratio 40:1, temperature program starting temperature 100°C, hold for 3 min; increase to 135°C at a rate of 10°C per minute, hold for 8 min; increase to 170°C at a rate of 12°C per minute, hold for 4 min; increase to 220°C at a rate of 15°C per minute, hold for 4 min.

[0029] Step 3: The mass spectrometer adopts EI mode, electron energy 70 eV, scanning mass range 30-550; transmission line temperature 280°C, ion source temperature 230°C.

[0030] Step 4: Establish a regression equation between the mass spectrum intensity of cinnamaldehyde and the concentration of cinnamaldehyde; substitute the obtained mass spectrum intensity of cinnamaldehyde into the regression equation to calculate the relative content of cinnamaldehyde in the germplasm to be tested. Further, calculate the percentage of cinnamaldehyde content in volatile oil content.

[0031] Wherein, the method for establishing the regression equation of cinnamaldehyde comprises the following steps: ① Prepare cinnamaldehyde standard solutions with concentrations of 0.0813 mg / mL, 0.1627 mg / mL, 1.6168 mg / mL, 3.2536 mg / mL, 6.5072 mg / mL, 13.0144 mg / mL, and 16.2680 mg / mL, with an injection volume of 1 uL.

[0032] ②Trace1300-TSQ8000 gas chromatography-mass spectrometry was used for determination. The chromatographic column, chromatographic conditions and detection and analysis methods were the same as above.

[0033] ③Through gas chromatography-mass spectrometry analysis, the total ion map, mass spectrum information and regression equation of the cinnamaldehyde standard were obtained. The regression equation of cinnamaldehyde is: Y=2268936277.15310x-55058931.85130, where x represents the mass spectrum intensity of cinnamaldehyde and Y represents the cinnamaldehyde content of the sample. Figure 4 This is the peak diagram of cinnamaldehyde reference substance.

[0034] Using the above method (dyeing screening, volatile oil extraction and cinnamaldehyde content detection), 100-200 cinnamon plants were screened from each cinnamon producing area, totaling about 2,000-3,000 plants. First, 2-4 plants with the darkest color were screened by dyeing, and then the content was determined and verified (volatile oil and cinnamaldehyde content detection). All of them conformed to the rule that the darker the color, the higher the corresponding volatile oil and cinnamaldehyde content. Among them, Figure 2This is a colorimetric diagram of the reaction of 100 cinnamon phloem plants from one of the production areas with a mixed solution of vanillin, methanolic sulfuric acid and sodium hydroxide. Plant No. 96 is the darkest stained one.

[0035] <Example 1> 35 seven-year-old cinnamon plants (numbered Ccas01~Ccas35) from the planting base were selected, and 4 candidate excellent germplasms (numbered Ccas-03, Ccas-04, Ccas-10 and Ccas30) were selected through <staining screening>; the volatile oil of the cinnamon plants was extracted, the volatile oil content was determined, and the cinnamaldehyde content in the volatile oil was detected by gas chromatography-mass spectrometry, and the percentage of cinnamaldehyde content in the volatile oil content was calculated (as shown in Table 1). It was found that the average volatile oil content of the 35 cinnamon germplasms was 2.15%, among which the volatile oil content of Ccas-04 was the highest, reaching 3.50%, followed by Ccas-03 germplasm, which was 3.16%, and the cinnamaldehyde content was higher than 75%, which met the judgment standard of "volatile oil content ≥2%, and cinnamaldehyde content accounted for volatile oil content percentage ≥75%". Therefore, they were judged to be cinnamon germplasms suitable for making cinnamaldehyde-flavored essential oil. This shows that the identification results of the method of the present invention are consistent with those of the direct identification method. Among them, the planting base for this experiment is cinnamon in Luoxiu Town, Guiping City, Guangxi, which belongs to Xijiang cinnamon.

[0036] Table 1 The volatile oil content and cinnamaldehyde content of Cinnamomum cassia Sample No. Oil content% Cinnamaldehyde content% Sample No. Oil content% Cinnamaldehyde content% Ccas-01 2.68 73.06 Ccas-19 1.57 67.92 Ccas-02 2.53 72.03 Ccas-20 1.75 75.54 Ccas-03 3.16 75.27 Ccas-21 2.47 59.83 Ccas-04 3.50 75.71 Ccas-22 2.65 68.56 Ccas-05 1.37 63.57 Ccas-23 2.65 67.53 Ccas-06 2.48 65.49 Ccas-24 1.82 70.95 Ccas-07 2.52 74.26 Ccas-25 2.27 70.32 Ccas-08 1.68 74.70 Ccas-26 1.97 64.52 Ccas-09 1.80 78.14 Ccas-27 2.50 71.67 Ccas-10 2.05 80.54 Ccas-28 2.05 72.29 Ccas-11 2.28 59.99 Ccas29 1.72 68.49 Ccas-12 2.68 48.62 Ccas-30 2.00 82.80 Ccas-13 2.30 58.12 Ccas-31 2.22 68.30 Ccas-14 1.98 70.71 Ccas-32 2.07 68.35 Ccas-15 1.73 66.67 Ccas-33 1.77 61.09 Ccas-16 2.05 57.08 Ccas-34 1.97 59.45 Ccas-17 2.25 54.10 Ccas-35 1.50 75.57 Ccas-18 1.30 58.82 <Example 2> Thirty-two seven-year-old cinnamon plants (numbered Ccas36~Ccas67) were selected from the planting base, and three excellent candidate germplasms (numbered Ccas-37, Ccas-49 and Ccas-51) were selected through <staining screening>; their volatile oils were extracted, the volatile oil content was determined, and the cinnamaldehyde content in the volatile oil was detected by gas chromatography-mass spectrometry, and the percentage of cinnamaldehyde content in the volatile oil content was calculated (as shown in Table 2). It was found that the average volatile oil content of the 32 cinnamon germplasms was 2.64%, among which the volatile oil content of Ccas-37 reached 3.18%, the volatile oil content of Ccas-51 and Ccas-49 was 3.03% and 2.30%, and the cinnamaldehyde content was 78.78%, 83.64% and 75.95% respectively; all of them met the judgment standard of "volatile oil content ≥2%, and cinnamaldehyde content in volatile oil content ≥75%", and they were judged to be cinnamon germplasms suitable for making cinnamaldehyde-flavored essential oil. This shows that the identification results of the method of the present invention are consistent with those of the direct identification method. Among them, the planting base of this experiment is cinnamon in Gaoche Township, Hua'an County, Fujian Province, which belongs to Qinghua cinnamon. Among them, Figure 3 This is the total ion chromatogram of the essential oil from the candidate excellent germplasm Ccas-49.

[0037] Table 2 Content of volatile oil and cinnamaldehyde in Qinghua Cinnamomum cassia Sample No. Oil content% Cinnamaldehyde content% Sample No. Oil content% Cinnamaldehyde content% Ccas-36 2.41 67.74 Ccas-47 3.46 72.20 Ccas-37 3.18 78.78 Ccas-48 2.80 59.39 Ccas-38 1.98 74.24 Ccas-49 2.30 75.95 Ccas-39 3.54 69.08 Ccas-50 1.42 68.76 Ccas-40 2.61 54.50 Ccas-51 3.03 83.64 Ccas-41 2.07 66.50 Ccas-52 2.93 70.05 Ccas-42 3.12 68.49 Ccas-53 2.97 63.44 Ccas-43 2.18 60.17 Ccas-54 3.07 64.37 Ccas-44 2.78 69.32 Ccas-55 2.97 71.43 Ccas-45 3.86 69.26 Ccas-56 2.43 68.73 Ccas-46 2.95 68.51 Ccas-57 2.21 65.14 <Example 3> Eight seven-year-old cinnamon plants (numbered Ccas68~Ccas75) from the planting base were selected, and one candidate excellent germplasm (numbered Ccas-69) was selected through <staining screening>; their volatile oils were extracted, the volatile oil content was determined, and the cinnamaldehyde content in the volatile oil was detected by gas chromatography-mass spectrometry, and the percentage of cinnamaldehyde content in the volatile oil content was calculated (as shown in Table 3). It was found that the average volatile oil content of the eight cinnamon germplasms was 2.80%, of which the volatile oil content of Ccas-69 was 2.01%, and the cinnamaldehyde content was 75.40%; it met the judgment standard of "volatile oil content ≥2%, and cinnamaldehyde content accounted for volatile oil content percentage ≥75%", and it was judged to be a cinnamon germplasm suitable for making cinnamaldehyde-flavored essential oil. This shows that the identification results of the method of the present invention are consistent with the results of the direct identification method. Among them, the planting bases of this experiment are Dongxing City and Fangchenggang Cinnamon in Guangxi, which belong to Dongxing Cinnamon.

[0038] Table 3 Volatile oil content and cinnamaldehyde content of Dongxing cinnamon Sample No. Oil content% Cinnamaldehyde content% Sample No. Oil content% Cinnamaldehyde content% Ccas-68 1.28 87.23 Ccas-72 4.00 67.25 Ccas-69 2.01 75.40 Ccas-73 4.52 69.86 Ccas-70 2.54 65.88 Ccas-74 2.53 73.22 Ccas-71 2.53 67.64 Ccas-75 3.02 52.31 <Comparative Example 1> A method for rapid identification of cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, based on Example 1, wherein the formula of the color developing solution in <staining screening> is a mixed solution of 40% sulfuric acid methanol solution containing 1% vanillin and 1 mol / L sodium hydroxide solution (3:1 by volume).

[0039] Except for the formulation of the developer solution, the others are the same as in Example 1.

[0040] The method of this comparative example cannot achieve a positive correlation between color depth and content, and cannot distinguish the differences between different germplasms, resulting in an inability to accurately determine the candidate excellent germplasms suitable for producing cinnamaldehyde-flavored essential oil.

[0041] <Comparative Example 2> A method for rapid identification of cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, based on Example 1, wherein the formula of the color developing solution in <staining screening> is a mixed solution of 5% sulfuric acid methanol solution containing 5% vanillin and 1 mol / L sodium hydroxide solution (3:1 by volume).

[0042] Except for the formulation of the developer solution, the others are the same as in Example 1.

[0043] The method of this comparative example cannot achieve a positive correlation between color depth and content, and cannot distinguish the differences between different germplasms, resulting in an inability to accurately determine the candidate excellent germplasms suitable for producing cinnamaldehyde-flavored essential oil.

[0044] <Comparative Example 3> A method for rapid identification of cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, based on Example 1, wherein the formula of the color developing solution in <staining screening> is a mixed solution of 40% sulfuric acid ethanol solution containing 5% vanillin and 1 mol / L sodium hydroxide solution (3:1 by volume).

[0045] Except for the formulation of the developer solution, the others are the same as in Example 1.

[0046] The color of the dyeing liquid in the samples of this comparative example is dark and dark, which makes it impossible to accurately determine the candidate excellent germplasm suitable for producing cinnamaldehyde-flavored essential oil.

[0047] <Comparative Example 4> A method for rapid identification of cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil. Based on Example 1, the formula of the color developing solution in <staining screening> is a 40% sulfuric acid methanol solution containing 5% vanillin.

[0048] Except for the formulation of the developer solution, the others are the same as in Example 1.

[0049] The color of the dyeing solution in the sample of this comparative example will continue to deepen as the dyeing time increases, resulting in the inability to accurately determine the candidate excellent germplasm suitable for producing cinnamaldehyde-flavored essential oil.

[0050] <Comparative Example 5> A method for rapid identification of cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, based on Example 1, wherein the dyed material in <dyeing screening> is a square of 5 mm×5 mm.

[0051] Except for the material size, everything else is the same as Example 1.

[0052] The dyeing solutions of this comparative example have similar colors, and the differences between different germplasms cannot be distinguished, resulting in the inability to accurately determine the candidate excellent germplasms suitable for producing cinnamaldehyde-flavored essential oil.

[0053] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes, and they can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A color developing solution for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, characterized in that: The color developing solution includes a mixture of 40% sulfuric acid methanol solution containing 4-6% vanillin and 1 mol / L sodium hydroxide solution in a volume ratio of 3:

1.

2. A method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde-flavored essential oil, characterized in that: Using the developer as claimed in claim 1, comprising the following steps: S1. Collect bark from 7-10 year old cinnamon plants at a height of 1-1.5 m above the ground. The bark size is 1-2 cm 2 of square; S2, preparing a 40% sulfuric acid methanol solution containing 4-6% vanillin, and then mixing it with a 1 mol / L sodium hydroxide solution in a volume ratio of 3:1 to obtain the color developing solution; S3, adding the color developing solution to the collected bark for reaction, transferring the color developing solution after the reaction, comparing the color depth of the color developing solution after the reaction, and screening out cinnamon plants with a color depth reaching a certain degree, which are candidate germplasms suitable for producing cinnamaldehyde-flavored essential oil.

3. The method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde aroma essential oil as claimed in claim 2, characterized in that, The following steps are also included: S4, detecting the volatile oil content and cinnamaldehyde content of the candidate germplasm screened out in step S3, and calculating the mass ratio of cinnamaldehyde to volatile oil; S5. Select candidate germplasms with volatile oil content ≥ 2% and cinnamaldehyde content as a percentage of volatile oil content ≥ 75%.

4. The method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde aroma essential oil as claimed in claim 2, characterized in that, In step S3, the color depth reaching a certain degree specifically means that the color depth reaches the color depth of the reaction between the cinnamaldehyde standard of 5 mg / mL and the color developing solution.

5. The method for rapidly identifying cinnamon germplasm suitable for producing cinnamaldehyde aroma essential oil as claimed in claim 2, characterized in that, The content of cinnamaldehyde was detected by gas chromatography-mass spectrometry, wherein the regression equation of cinnamaldehyde was: Y=2268936277.15310 x-55058931.85130.

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