A systemic lupus erythematosus autoantibody detection device and method

Abstract

The present invention relates to the field of immunoassay technology, and in particular to a systemic lupus erythematosus autoantibody detection device and method. The systemic lupus erythematosus autoantibody detection device includes a detection box, a cleaning component, a detection component, a heating mechanism, a uniform component, two support components and a holding component, and the heating mechanism includes a first heating component, a second heating component and a third heating component. The cleaning component is used to clean the detection component, and when it is stained with corrosive liquid, it can be quickly cleaned. The detection component is used to detect systemic lupus erythematosus autoantibodies. The heating mechanism can preheat the detection component and the uniform component so that the detection component and the uniform component can be heated, thereby speeding up the detection speed, and then providing a treatment plan in time. The uniform component can quickly prepare a cleaning preparation liquid, thereby speeding up the detection speed, and at the same time preventing the heating mechanism from being blocked. The two support components can support the detection box and start the heating mechanism.

Description

Technical Field

[0001] The present invention relates to the technical field of immunoassay, and in particular to a systemic lupus erythematosus autoantibody detection device and method. Background Art

[0002] Systemic lupus erythematosus is an autoimmune disease, one of the characteristics of which is the production of a large number of autoantibodies in the patient's body. Common systemic lupus erythematosus autoantibodies include antinuclear antibodies, double-stranded DNA antibodies, Sm antibodies, etc. One of the methods for detecting these autoantibodies is to use an automated immunological detection device, among which enzyme-linked immunosorbent assay and fluorescent immunoassay are the most commonly used. Among them, the current enzyme-linked immunosorbent assay takes a long time, and during the experiment, collisions are inevitable when personnel are pouring the experimental liquid, causing the test liquid to be contaminated on the experimenter, and then causing injury to the experimenter. Summary of the invention

[0003] Based on this, it is necessary to provide a systemic lupus erythematosus autoantibody detection device and method to solve at least one of the above technical problems.

[0004] A systemic lupus erythematosus autoantibody detection device comprises a detection box, a cleaning component, a detection component, a heating mechanism, a uniform component, two supporting components and a receiving component. The detection box is hollow inside to form a storage space. The cleaning component, the detection component, the heating mechanism, the uniform component, the two supporting components and the receiving component are all received in the storage space. A fixed plate is installed at the bottom of the storage space. The cleaning component is installed at one end of the top of the fixed plate, the receiving component is installed at the other end of the top of the fixed plate, the detection component is installed at one side of the fixed plate, the heating mechanisms are all installed at the other side of the fixed plate, the uniform component is installed on the side wall of the heating mechanism, and the two supporting components are respectively installed on the two side walls of the storage space. The heating mechanism comprises a first heating component, a second heating component and a third heating component. The first heating component, the second heating component and the third heating component are all installed on the other side of the fixed plate. The first heating component is located on a side adjacent to the cleaning component, the third heating component is located on a side adjacent to the receiving component, the second heating component is located between the first heating component and the third heating component, and the uniform component is slidably inserted in the second heating component.

[0005] The cleaning component is used to clean the detection component and can be quickly cleaned when it is stained with corrosive liquid. The detection component is used to detect systemic lupus erythematosus autoantibodies. The heating mechanism can preheat the detection component and the uniform component so that the detection component and the uniform component can be heated up, thereby speeding up the detection speed and obtaining results faster, thereby providing treatment plans in a timely manner. The uniform component can quickly prepare a cleaning preparation liquid, thereby speeding up the detection speed and preventing the heating mechanism from being blocked. The two support components can support the detection box and start the heating mechanism.

[0006] Preferably, the detection box comprises a detection box body, a hinged rod and an upper cover, the hinged rod is rotatably mounted on the top of the side wall of the detection box body, and the upper cover is fixedly connected to the hinged rod.

[0007] Preferably, the cleaning assembly includes a cleaning trough, a water tank, a drain pipe and a valve. The cleaning trough is fixedly installed at one end of the top of the fixed plate, the water tank is installed on the side wall of the storage space, the drain pipe is inserted in the water tank, and the valve is installed on the top of the drain pipe.

[0008] Preferably, the detection assembly includes three fixed rods, a clamp and an ELISA plate, one end of the three fixed rods are inserted into the side wall of the storage space away from the hinged rod, the clamp is fixedly connected to the other end of the three fixed rods, the ELISA plate is installed on the top of the clamp, a plurality of detection holes are opened on the top of the ELISA plate, and a recovery groove is installed on the top of the fixed plate, and the recovery groove is located directly below the clamp.

[0009] Preferably, the first heating component, the second heating component and the third heating component all include a heating body, an air guide rod made of an elastic material, two L-shaped connecting rods, a fixed block, a first arc-shaped clamp made of an elastic material and a liquid absorbent piece, the bottom of the heating body is connected to the side wall of the fixing plate adjacent to the hinge rod, one end of the air guide rod is inserted in the heating body, one end of the two L-shaped connecting rods are inserted in the heating body, and the two L-shaped connecting rods are arranged facing each other, the two ends of the fixed block are respectively fixedly connected to the other ends of the two L-shaped connecting rods, the first arc-shaped clamp is fixedly installed on the side wall of the fixed block, the liquid absorbent piece is clamped in the first arc-shaped clamp, and the other end of the air guide rod is inserted in the liquid absorbent piece.

[0010] Preferably, a first air outlet is provided at the bottom of one end of the air guide rod of the first heating component adjacent to the liquid absorbing member, and the first air outlet is arranged obliquely toward the ELISA plate; a third air outlet is provided at the bottom of one end of the air guide rod of the third heating component adjacent to the liquid absorbing member, and the third air outlet is arranged obliquely toward the ELISA plate; a second air outlet is provided at the bottom of one end of the air guide rod of the second heating component adjacent to the liquid absorbing member, and the second air outlet is arranged obliquely toward the ELISA plate; each liquid absorbing member includes a retaining ring, a liquid absorbing rod made of plastic material, a liquid absorbing head and an elliptical recovery air bag, and the retaining ring The clamp is arranged in the first arc-shaped clamp, the liquid suction rod is inserted into the clamping ring, the liquid suction head is installed at the bottom of the liquid suction rod, the recovery air bag is installed at the top of the liquid suction rod, and an air inlet slit is opened in the middle of the liquid suction rod, and the air inlet slit is located above the clamping ring, and a clamping groove is opened at the bottom of one end of each air guide rod away from the heating body, and fixing plates are installed on both side walls of each air guide rod, and one side wall of each fixing plate is fixedly connected to the adjacent side wall of the heating body, the first air outlet is located in the liquid suction part of the first heating component, and the inner side wall of each clamping groove is formed with a push-against inclined surface.

[0011] Preferably, an inverted T-shaped starting groove is opened on the side wall of the second heating component, and the uniform component is slidably inserted in the top of the starting groove. The uniform component includes a rocking rod, a pushing rod, a second arc clamp and a mixing tube. The rocking rod is slidably inserted in the top of the starting groove, and the lower end of the pushing rod is fixedly connected to the top of the end of the rocking rod away from the starting groove, and the top of the pushing rod is clamped in the clamping groove of the air guide rod. The second arc clamp is fixedly connected to the end of the rocking rod away from the starting groove, and the middle part of the mixing tube is opened in the second arc clamp.

[0012] Preferably, each support assembly includes an upper support rod, a hinge column and a lower support rod, the bottom of the upper support rod is hinged to the side wall of the storage space, the hinge column is rotatably installed on the top of the upper support rod, the top of the lower support rod is hinged to the upper cover, and the hinge column is rotatably installed on the bottom of the lower support rod, wherein the support assembly adjacent to the cleaning tank side also includes an upper conductive block, a lower conductive block and a power supply, the upper conductive block is fixedly installed on the bottom of the upper support rod, the lower conductive block is fixedly installed on the top of the lower support rod, the power supply is installed on the side wall of the storage space, and the upper conductive block and the lower conductive block are both electrically connected to the power supply, and the heating bodies of the first heating assembly, the second heating assembly and the third heating assembly are all electrically connected to the power supply.

[0013] Preferably, the receiving assembly includes a fixing frame and a plurality of fixing boxes. The fixing frame is fixedly mounted on the other end of the top of the fixing plate, and the plurality of fixing boxes are fixedly mounted on the side wall of the fixing frame.

[0014] The present invention also provides a method for detecting systemic lupus erythematosus autoantibodies, the method using the above-mentioned systemic lupus erythematosus autoantibody detection device, the method comprising the following steps: Step S1: Fix the sample to be tested in the ELISA plate of the systemic lupus erythematosus autoantibody detection device.

[0015] Step S2: Add specific antibodies or antigens to allow the sample to be tested to specifically bind to the antibodies or antigens.

[0016] Step S3: prepare a cleaning solution to wash the unbound substances in the detection wells.

[0017] Step S4: Add detection antibody and allow it to bind to the molecule to be detected to form an enzyme-labeled complex.

[0018] Step S5: Wash again to remove unbound antibodies, add substrate to bind to the enzyme to produce a staining reaction, and measure the light intensity or color change of the staining product by spectroscopy to obtain the result.

[0019] The present invention provides an upper support rod and a lower support rod. When the upper cover of the detection box is opened, the upper support rod and the lower support rod will be separated, so that the upper support rod and the lower support rod are in a straight line, and the upper cover of the detection box is lifted up. The upper support rod and the lower support rod not only prevent the upper cover from being unsupported and the upper cover from being suddenly closed after an accident, which affects the detection result, but also can enable the circuit to be turned on when the upper conductive block and the lower conductive block are abutted together, and the heating bodies of the first heating component, the second heating component and the third heating component begin to heat the internal gas, and transport the internal hot gas to the inside of the liquid pipetting rod through the gas guide rod. The hot gas is used to preheat the inside of the liquid pipetting component, so that the liquid pipetting component can increase the temperature of the prepared liquid when extracting the prepared liquid, thereby accelerating the detection speed. By setting a heating body, when the liquid-absorbing parts of the first heating component and the third heating component are taken out from the first arc-shaped clamp, the hot gas inside the heating body will be blown toward the multiple detection holes to heat the ELISA plate, thereby accelerating the marking speed of the ELISA plate. At the same time, the hot gas blown out from the heating body can also blow out the excess corrosive culture fluid in the detection holes, thereby preventing the ELISA plate from having too much culture fluid in the detection holes when washing the plate, and then preventing the excess culture fluid from flowing out of the detection holes and contaminating the hands of the operators when disassembling the ELISA plate, thereby injuring the operators. At the same time, the recovery tank can collect excess culture fluid to prevent the culture fluid from scattering in the storage space. By setting a shaking rod, the liquid flows into the mixing tube under the traction of the liquid suction head, and is used to preheat the mixing tube. When the liquid suction parts of the first heating component, the second heating component and the third heating component absorb the cleaning mixing liquid and drop it into the mixing tube, the weight of the mixing tube rises, and the shaking rod will move downward. A shaking switch is installed at the bottom of the start slot, and the shaking rod will move downward and start the shaking switch, so that the shaking rod starts to shake, so that the cleaning mixing liquid can be mixed more evenly, the speed of washing the plate is accelerated, and then the speed of detection is accelerated. At the same time, the push rod can knock the air guide rod while shaking to prevent the air guide rod from being blocked. The structure of the present invention is ingenious, which can prevent accidents during detection, and at the same time can speed up the detection speed, get results faster, and provide treatment plans in time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a three-dimensional schematic diagram of an overall embodiment.

[0021] Figure 2 It is a three-dimensional schematic diagram of another viewing angle of an embodiment.

[0022] Figure 3 FIG. 4 is a three-dimensional schematic diagram of a second heating component according to an embodiment.

[0023] Figure 4 An embodiment Figure 1 A magnified schematic diagram of center A.

[0024] Figure 5 An embodiment Figure 1Enlarged diagram after removing the fixing plate at B.

[0025] Figure 6 An embodiment Figure 2 Enlarged diagram after removing the fixing plate at C in the middle.

[0026] Figure 7 Schematic diagram of a three-dimensional liquid absorbent member according to an embodiment.

[0027] Figure 8 It is a three-dimensional schematic diagram of the air guide rod of the first heating component of an embodiment.

[0028] In the figure: 10, test box; 11, storage space; 12, fixing plate; 13, test box body; 14, hinged rod; 15, upper cover; 20, cleaning component; 21, cleaning tank; 22, water storage tank; 23, drain pipe; 24, valve; 30, test component; 31, fixing rod; 32, clamp; 33, ELISA plate; 34, test hole; 35, recovery tank; 40, heating mechanism; 41, first heating component; 42, second heating component; 43, third heating component; 44, heating body; 45, air guide rod; 46, connecting rod; 47, fixing block; 48, first arc clamp; 49, Liquid-absorbing component; 420, starting slot; 450, positioning slot; 451, pushing inclined plane; 452, fixing plate; 490, positioning ring; 491, liquid-absorbing rod; 492, liquid-absorbing head; 493, recovery airbag; 494, air inlet slit; 499, first air outlet; 50, uniform component; 51, shaking rod; 52, pushing rod; 53, second arc-shaped clamp; 54, adjusting pipe; 60, supporting component; 61, upper supporting rod; 62, hinged column; 63, lower supporting rod; 64, upper conductive block; 65, lower conductive block; 66, power supply; 70, supporting component; 71, fixing frame; 72, fixing box. DETAILED DESCRIPTION

[0029] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0030] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0032] An embodiment provided by the present invention is as follows: Figures 1 to 8 As shown, a systemic lupus erythematosus autoantibody detection device includes a detection box 10, a cleaning component 20, a detection component 30, a heating mechanism 40, a uniform component 50, two support components 60 and a receiving component 70. The detection box 10 is hollow inside to form a storage space 11. The cleaning component 20, the detection component 30, the heating mechanism 40, the uniform component 50, the two support components 60 and the receiving component 70 are all received in the receiving space 11. A fixing plate 12 is installed at the bottom of the receiving space 11. The cleaning component 20 is installed at one end of the top of the fixing plate 12, the receiving component 70 is installed at the other end of the top of the fixing plate 12, the detection component 30 is installed on one side of the fixing plate 12, and the heating mechanism 40 is installed on the other side of the fixing plate 12. 0 are all installed on the other side of the fixing plate 12, the uniform component 50 is installed on the side wall of the heating mechanism 40, and the two supporting components 60 are respectively installed on the two side walls of the storage space 11. The heating mechanism 40 includes a first heating component 41, a second heating component 42 and a third heating component 43. The first heating component 41, the second heating component 42 and the third heating component 43 are all installed on the other side of the fixing plate 12, the first heating component 41 is located on the side adjacent to the cleaning component 20, the third heating component 43 is located on the side adjacent to the placing component 70, the second heating component 42 is located between the first heating component 41 and the third heating component 43, and the uniform component 50 is slidably inserted in the second heating component 42.

[0033] The cleaning component 20 is used to clean the detection component 30, and can be quickly cleaned when it is stained with corrosive liquid. The detection component 30 is used to detect systemic lupus erythematosus autoantibodies. The heating mechanism 40 can preheat the detection component 30 and the uniform component 50, so that the detection component 30 and the uniform component 50 can increase the temperature, thereby accelerating the detection speed, obtaining results faster, and providing treatment plans in time. The uniform component 50 can quickly prepare a cleaning preparation liquid, thereby accelerating the detection speed and preventing the heating mechanism 40 from being blocked. The two support components 60 can support the detection box 10 and start the heating mechanism 40.

[0034] like Figure 1 and Figure 2 As shown, the detection box 10 includes a detection box body 13 , a hinge rod 14 and an upper cover 15 . The hinge rod 14 is rotatably mounted on the top of the side wall of the detection box body 13 , and the upper cover 15 is fixedly connected to the hinge rod 14 .

[0035] like Figure 1 to Figure 2 As shown, the cleaning assembly 20 includes a cleaning tank 21, a water tank 22, a drain pipe 23 and a valve 24. The cleaning tank 21 is fixedly installed at one end of the top of the fixed plate 12, the water tank 22 is installed on the side wall of the storage space 11, the drain pipe 23 is inserted in the water tank 22, and the valve 24 is installed on the top of the drain pipe 23.

[0036] like Figure 1 and Figure 2 As shown, the detection component 30 includes three fixed rods 31, a clamp 32 and an ELISA plate 33. One ends of the three fixed rods 31 are inserted into the side wall of the storage space 11 away from the hinge rod 14. The clamp 32 is fixedly connected to the other ends of the three fixed rods 31. The ELISA plate 33 is installed on the top of the clamp 32. A plurality of detection holes 34 are opened on the top of the ELISA plate 33. A recovery groove 35 is installed on the top of the fixed plate 12, and the recovery groove 35 is located directly below the clamp 32.

[0037] like Figures 2 to 6 As shown, the first heating component 41, the second heating component 42 and the third heating component 43 all include a heating body 44, an air guide rod 45 made of elastic material, two L-shaped connecting rods 46, a fixed block 47, a first arc-shaped clamp 48 made of elastic material and a liquid absorbent part 49. The bottom of the heating body 44 is connected to the side wall of the fixed plate 12 adjacent to the hinge rod 14. One end of the air guide rod 45 is inserted in the heating body 44, one end of the two L-shaped connecting rods 46 are both inserted in the heating body 44, and the two L-shaped connecting rods 46 are arranged opposite to each other. The two ends of the fixed block 47 are respectively fixedly connected to the other ends of the two L-shaped connecting rods 46, the first arc-shaped clamp 48 is fixedly installed on the side wall of the fixed block 47, the liquid absorbent part 49 is clamped in the first arc-shaped clamp 48, and the other end of the air guide rod 45 is inserted in the liquid absorbent part 49.

[0038] like Figures 2 to 4 and Figure 8As shown, a first air outlet 499 is provided at the bottom of one end of the air guide rod 45 of the first heating component 41 adjacent to the liquid absorbing member 49, and the first air outlet 499 is arranged obliquely toward the ELISA plate 33. A third air outlet (not shown) is provided at the bottom of one end of the air guide rod 45 of the third heating component 43 adjacent to the liquid absorbing member 49, and the third air outlet is arranged obliquely toward the ELISA plate 33. A second air outlet (not shown) is provided at the bottom of one end of the air guide rod 45 of the second heating component 42 adjacent to the liquid absorbing member 49, and the second air outlet is arranged obliquely toward the ELISA plate 33. Each liquid absorbing member 49 includes a retaining ring 490, a liquid absorbing rod 491 made of plastic material, a liquid absorbing head 492 and an elliptical recovery air bag 493, and the retaining ring 490 is clamped on In the first arc clamp 48, the liquid suction rod 491 is inserted into the retaining ring 490, the liquid suction head 492 is installed at the bottom of the liquid suction rod 491, the recovery air bag 493 is installed at the top of the liquid suction rod 491, and an air inlet slit 494 is opened in the middle of the liquid suction rod 491, and the air inlet slit 494 is located above the retaining ring 490, and a retaining groove 450 is opened at the bottom of one end of each air guide rod 45 away from the heating body 44, and each side wall of the air guide rod 45 is installed with a fixing plate 452, and one side wall of each fixing plate 425 is fixedly connected to the adjacent side wall of the heating body 44, and the first air outlet 499 is located in the liquid suction part 49 of the first heating component 41, and the inner wall of each retaining groove 450 is formed with a push-against inclined surface 451.

[0039] like Figures 5 to 7 As shown, an inverted T-shaped starting groove 420 is opened on the side wall of the second heating component 42, and the uniform component 50 is slidably inserted in the top of the starting groove 420. The uniform component 50 includes a shaking rod 51, a pushing rod 52, a second arc clamp 53 and a mixing tube 54. The shaking rod 51 is slidably inserted in the top of the starting groove 420, and the lower end of the pushing rod 52 is fixedly connected to the top of the end of the shaking rod 51 away from the starting groove 420, and the top of the pushing rod 52 is clamped in the clamping groove 450 of the air guide rod 45, the second arc clamp 53 is fixedly connected to the end of the shaking rod 51 away from the starting groove 420, and the middle part of the mixing tube 54 is opened in the second arc clamp 53.

[0040] like Figure 1 and Figure 4As shown, each support assembly 60 includes an upper support rod 61, a hinge column 62 and a lower support rod 63, the bottom of the upper support rod 61 is hinged to the side wall of the storage space 11, the hinge column 62 is rotatably installed on the top of the upper support rod 61, the top of the lower support rod 63 is hinged to the upper cover 15, and the hinge column 62 is rotatably installed on the bottom of the lower support rod 63, wherein the support assembly 60 adjacent to the side of the cleaning tank 21 also includes an upper conductive block 64, a lower conductive block 65 and a power supply 66, the upper conductive block 64 is fixedly installed on the bottom of the upper support rod 61, the lower conductive block 65 is fixedly installed on the top of the lower support rod 63, the power supply 66 is installed on the side wall of the storage space 11, and the upper conductive block 64 and the lower conductive block 65 are both electrically connected to the power supply 66, and the heating bodies 44 of the first heating assembly 41, the second heating assembly 42 and the third heating assembly 43 are all electrically connected to the power supply 66.

[0041] like Figure 2 As shown, the receiving assembly 70 includes a fixing frame 71 and a plurality of fixing boxes 72 . The fixing frame 71 is fixedly mounted on the other end of the top of the fixing plate 12 , and the plurality of fixing boxes 72 are fixedly mounted on the side walls of the fixing frame 71 .

[0042] The present invention also provides a method for detecting systemic lupus erythematosus autoantibodies, the method using the above-mentioned systemic lupus erythematosus autoantibody detection device, the method comprising the following steps: Step S1: Fix the sample to be tested in the ELISA plate 33 of the systemic lupus erythematosus autoantibody detection device.

[0043] Step S2: Add specific antibodies or antigens (not shown) to allow the sample to be tested to specifically bind to the antibodies or antigens.

[0044] Step S3: prepare a cleaning solution to wash the unbound substances in the detection holes 34 .

[0045] Step S4: Add detection antibody and allow it to bind to the molecule to be detected to form an enzyme-labeled complex.

[0046] Step S5: Wash again to remove unbound antibodies, add substrate to bind to the enzyme to produce a staining reaction, and measure the light intensity or color change of the staining product by spectroscopy to obtain the result.

[0047] During installation: install the cleaning component 20 on one end of the top of the fixed plate 12, the supporting component 70 on the other end of the top of the fixed plate 12, the detecting component 30 on one side of the fixed plate 12, the heating mechanism 40 on the other side of the fixed plate 12, the uniform component 50 on the side wall of the heating mechanism 40, the two supporting components 60 on the two side walls of the storage space 11, the first heating component 41, the second heating component 42 and the third heating component 43 on the other side of the fixed plate 12, the hinged rod 14 on the top of the side wall of the detection box body 13, the cleaning tank 21 on the top of the fixed plate 12, the water tank 22 on the side wall of the storage space 11, the valve 24 on the top of the drain pipe 23. The ELISA plate 33 is installed on the top of the clamp 32, the first arc clamp 48 is fixedly installed on the side wall of the fixed block 47, the pipette head 492 is installed on the bottom of the pipette rod 491, the recovery airbag 493 is installed on the top of the pipette rod 491, the hinge column 62 is rotatably installed on the top of the upper support rod 61, the upper conductive block 64 is fixedly installed on the bottom of the upper support rod 61, the lower conductive block 65 is fixedly installed on the top of the lower support rod 63, the power supply 66 is installed on the side wall of the storage space 11, the fixing frame 71 is fixedly installed on the other end of the top of the fixing plate 12, and multiple fixing boxes 72 are all fixedly installed on the side wall of the fixing frame 71.

[0048] During use: 1. Open the upper cover 15 of the detection box 10, the upper support rod 61 and the lower support rod 63 will separate, so that the upper support rod 61 and the lower support rod 63 are in a straight line, and the upper cover 15 of the detection box 10 is lifted up. The upper support rod 61 and the lower support rod 63 not only prevent the upper cover 15 from being unsupported and the sudden closing of the upper cover 15 after an accident affects the detection result, but also when the upper conductive block 64 and the lower conductive block 65 are abutted together, the circuit is turned on, and the heating bodies 44 of the first heating component 41, the second heating component 42 and the third heating component 43 begin to heat the internal gas, and transport the internal hot gas to the inside of the liquid absorption rod 491 through the gas guide rod 45. The hot gas is used to preheat the inside of the liquid absorption component 49, so that the liquid absorption component 49 can increase the temperature of the prepared liquid when extracting the prepared liquid, thereby speeding up the detection speed.

[0049] 2. When the liquid absorbing member 49 of the first heating component 41 and the third heating component 43 is taken out from the first arc-shaped clamp 48 for experiment, the staff leaves the detection box 10, and the hot gas inside the heating body 44 will blow to the multiple detection holes 34 to heat the ELISA plate 33, thereby accelerating the marking speed of the ELISA plate 33. At the same time, the hot gas blown out from the heating body 44 can also blow out the excess corrosive culture solution inside the detection holes 34. After the excess culture solution is blown away, the hot gas blown out from the heating body 44 will not blow out the culture solution inside the detection holes 34. At the same time, the height of the ELISA plate 33 is lower than the height of the detection box body 13, and the excess culture solution will not be blown out of the detection box body 13, so as to prevent the ELISA plate 33 from having too much culture solution inside the detection holes 34 when washing the plate, and then prevent the excess culture solution from flowing out of the detection holes 34 and contaminating the hands of the operator when disassembling the ELISA plate 33, thereby injuring the operator. At the same time, the recovery tank 35 can collect the excess culture solution to prevent the culture solution from scattering in the storage space 11.

[0050] 3. When the liquid absorbing member 49 of the second heating assembly 42 is taken out from the second arc-shaped clamp 53 for the experiment, the staff leaves the detection box 10, and the hot gas inside the heating body 44 will be blown to the multiple detection holes 34 (so that the hot gas inside the air guide rod 45 can be blown to the multiple detection holes 34 through the second air outlet, and the hot gas blown out of the heating body 44 will not blow out the culture solution inside the detection holes 34. At the same time, the height of the ELISA plate 33 is lower than the height of the detection box body 13, and the excess culture solution will not be blown out of the detection box body 13) to heat the ELISA plate 33, thereby accelerating the marking speed of the ELISA plate 33, and entering the second heating. The hot gas of the liquid suction rod 491 in the thermal component 42 flows out from the liquid suction head 492, and flows into the mixing pipe 54 under the traction of the liquid suction head 492, and is used to preheat the mixing pipe 54. When the liquid suction parts 49 of the first heating component 41, the second heating component 42 and the third heating component 43 absorb the cleaning mixing liquid and drop it into the mixing pipe 54, the weight of the mixing pipe 54 increases, and the shaking rod 51 will move downward. A shaking switch (not shown) is installed at the bottom of the start slot 420, and the shaking rod 51 will move downward, and the shaking switch is started, so that the shaking rod 51 starts to shake, so that the cleaning mixing liquid can be mixed more quickly and evenly. To speed up the plate washing speed and thus speed up the detection speed, the shaking rod 51 stops shaking for a period of time, and the mixing tube 54 is taken out to wash the ELISA plate 33. At the same time, after the shaking rod 51 moves downward, the end of the air guide rod 45 away from the heating body 44 returns to its original state downward, and after the shaking rod 51 moves downward to the limit, the push rod 52 is still inserted in the positioning groove 450, and the shaking rod 51 starts to shake, and the push rod 52 pushes the inclined surface 451 upward, so that the air guide rod 45 moves upward, and the top of the push rod 52 is separated from the positioning groove 450. After the top of the push rod 52 is separated from the positioning groove 450, the air guide rod 45 Restore to the original state, the push rod 52 follows the push rod 52 to swing and is used to repeatedly knock the bottom of the air guide rod 45 left and right. When knocking, the push rod 52 will not collide with the fixed plate 452 to prevent the air guide rod 45 from being blocked. At the same time, when the push rod 52 swings to repeatedly knock the air guide rod 45 left and right, the hot gas blown out from the inside of the air guide rod 45 flows out in a shaking manner. The hot gas blown out from the inside of the air guide rod 45 flows out in a shaking manner, which can more thoroughly blow out the excess corrosive culture fluid in the detection hole 34, thereby preventing excess culture fluid from flowing out of the detection hole 34 and contaminating the hands of the operator, thereby injuring the operator.After the shaking is completed, the shaking rod 51 returns to the middle of the starting groove 420, and the pushing rod 52 follows the shaking rod 51 to return to the middle of the starting groove 420. At this time, the pushing rod 52 will abut against the side wall of the air guide rod 45 and follow the shaking rod 51 to slide into the positioning groove 450. The fixing plates 452 on the two side walls of the air guide rod 45 can prevent the air guide rod 45 from undergoing a large displacement, and at the same time ensure that when the dispensing tube 54 is removed, the shaking rod 51 can return to its original shape upward, and continue to push the end of the air guide rod 45 away from the starting groove 420 to tilt upward, so that the hot gas inside the air guide rod 45 can be blown toward the multiple detection holes 34 through the second air outlet, which is used to heat the ELISA plate 33, thereby accelerating the marking speed of the ELISA plate 33.

[0051] The present invention sets an upper support rod 61 and a lower support rod 63. When the upper cover 15 of the detection box 10 is opened, the upper support rod 61 and the lower support rod 63 will separate, so that the upper support rod 61 and the lower support rod 63 are in a straight line, and the upper cover 15 of the detection box 10 is lifted up. The upper support rod 61 and the lower support rod 63 not only prevent the upper cover 15 from being unsupported and the upper cover 15 from being suddenly closed after an accident, which affects the detection result, but also can enable the circuit to be turned on when the upper conductive block 64 and the lower conductive block 65 are abutted together, and the heating bodies 44 of the first heating component 41, the second heating component 42 and the third heating component 43 begin to heat the internal gas, and transport the internal hot gas to the inside of the liquid suction rod 491 through the gas guide rod 45. The hot gas is used to preheat the inside of the liquid suction component 49, so that the liquid suction component 49 can increase the temperature of the prepared liquid when extracting the prepared liquid, thereby speeding up the detection speed. By setting up a heating body 44, when the liquid absorption parts 49 of the first heating component 41 and the third heating component 43 are taken out from the first arc clamp 48, the hot gas inside the heating body 44 will be blown toward the multiple detection holes 34 to heat the ELISA plate 33, thereby accelerating the marking speed of the ELISA plate 33. At the same time, the hot gas blown out from the heating body 44 can also blow out the excess corrosive culture fluid inside the detection holes 34, thereby preventing the ELISA plate 33 from having too much culture fluid inside the detection holes 34 when washing the plate, and thereby preventing the excess culture fluid from flowing out of the detection holes 34 and contaminating the hands of the operators when disassembling the ELISA plate 33, thereby injuring the operators. At the same time, the recovery tank 35 can collect excess culture fluid to prevent the culture fluid from scattering in the storage space 11. By setting a shaking rod 51, the liquid flows into the mixing pipe 54 under the traction of the liquid suction head 492, and is used to preheat the mixing pipe 54. When the liquid suction parts 49 of the first heating component 41, the second heating component 42 and the third heating component 43 absorb the cleaning mixing liquid and drop it into the mixing pipe 54, the weight of the mixing pipe 54 increases, and the shaking rod 51 will move downward. A shaking switch is installed at the bottom of the start slot 420. The shaking rod 51 will move downward and start the shaking switch, so that the shaking rod 51 starts to shake, so that the cleaning mixing liquid can be mixed more evenly, the speed of washing the plate is accelerated, and then the speed of detection is accelerated. At the same time, the push rod 52 can knock the air guide rod 45 while shaking to prevent the air guide rod 45 from being blocked. The structure of the present invention is ingenious, which can prevent accidents during detection, and at the same time can speed up the detection speed, get results faster, and then provide treatment plans in time.

[0052] All possible combinations of the various technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0053] The above-mentioned embodiments only express several embodiments of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be based on the attached claims.

Claims

1. A systemic lupus erythematosus autoantibody detection device, characterized in that: The invention comprises a detection box (10), a cleaning component (20), a detection component (30), a heating mechanism (40), a uniform component (50), two supporting components (60) and a receiving component (70); the detection box (10) is hollow inside to form a storage space (11); the cleaning component (20), the detection component (30), the heating mechanism (40), the uniform component (50), the two supporting components (60) and the receiving component (70) are all received in the storage space (11); a fixing plate (12) is installed at the bottom of the storage space (11); the cleaning component (20) is installed at one end of the top of the fixing plate (12); the receiving component (70) is installed at the other end of the top of the fixing plate (12); the detection component (30) is installed at one side of the fixing plate (12); and the heating mechanism (40) is installed at the fixing plate. On the other side of the fixing plate (12), the uniform component (50) is installed on the side wall of the heating mechanism (40), and the two supporting components (60) are respectively installed on the two side walls of the storage space (11). The heating mechanism (40) comprises a first heating component (41), a second heating component (42) and a third heating component (43). The first heating component (41), the second heating component (42) and the third heating component (43) are all installed on the other side of the fixing plate (12). The first heating component (41) is located on a side adjacent to the cleaning component (20), the third heating component (43) is located on a side adjacent to the placing component (70), the second heating component (42) is located between the first heating component (41) and the third heating component (43), and the uniform component (50) is slidably inserted into the second heating component (42).

2. The systemic lupus erythematosus autoantibody detection device according to claim 1, characterized in that: The detection box (10) comprises a detection box body (13), a hinged rod (14) and an upper cover (15); the hinged rod (14) is rotatably mounted on the top of the side wall of the detection box body (13); and the upper cover (15) is fixedly connected to the hinged rod (14).

3. The systemic lupus erythematosus autoantibody detection device according to claim 2, characterized in that: The cleaning assembly (20) comprises a cleaning tank (21), a water storage tank (22), a drain pipe (23) and a valve (24); the cleaning tank (21) is fixedly mounted on one end of the top of the fixing plate (12); the water storage tank (22) is mounted on the side wall of the storage space (11); the drain pipe (23) is inserted into the water storage tank (22); and the valve (24) is mounted on the top of the drain pipe (23).

4. The systemic lupus erythematosus autoantibody detection device according to claim 3, characterized in that: The detection assembly (30) comprises three fixed rods (31), a clamp (32) and an ELISA plate (33). One end of the three fixed rods (31) is inserted into a side wall of the storage space (11) away from the hinge rod (14). The clamp (32) is fixedly connected to the other ends of the three fixed rods (31). The ELISA plate (33) is mounted on the top of the clamp (32). A plurality of detection holes (34) are provided on the top of the ELISA plate (33). A recovery groove (35) is installed on the top of the fixed plate (12). The recovery groove (35) is located directly below the clamp (32).

5. The systemic lupus erythematosus autoantibody detection device according to claim 4, characterized in that: The first heating assembly (41), the second heating assembly (42) and the third heating assembly (43) each comprise a heating body (44), an air guide rod (45) made of an elastic material, two L-shaped connecting rods (46), a fixing block (47), a first arc-shaped clamp (48) made of an elastic material and a liquid absorbing member (49), wherein the bottom of the heating body (44) is connected to a side wall of the fixing plate (12) adjacent to the hinge rod (14), and one end of the air guide rod (45) is inserted into the heating body ( 44), one end of the two L-shaped connecting rods (46) are inserted into the heating body (44), and the two L-shaped connecting rods (46) are arranged opposite to each other, the two ends of the fixing block (47) are respectively fixedly connected to the other ends of the two L-shaped connecting rods (46), the first arc-shaped clamp (48) is fixedly installed on the side wall of the fixing block (47), the liquid absorption piece (49) is clamped in the first arc-shaped clamp (48), and the other end of the air guide rod (45) is inserted into the liquid absorption piece (49).

6. The systemic lupus erythematosus autoantibody detection device according to claim 5, characterized in that: A first air outlet (499) is provided at the bottom of one end of the air guide rod (45) of the first heating component (41) adjacent to the liquid absorbing member (49), and the first air outlet (499) is arranged obliquely toward the ELISA plate (33). A third air outlet is provided at the bottom of one end of the air guide rod (45) of the third heating component (43) adjacent to the liquid absorbing member (49), and the third air outlet is arranged obliquely toward the ELISA plate (33). A second air outlet is provided at the bottom of one end of the air guide rod (45) of the second heating component (42) adjacent to the liquid absorbing member (49), and the second air outlet is arranged obliquely toward the ELISA plate (33). Each liquid absorbing member (49) comprises a retaining ring (490), a liquid absorbing rod (491) made of plastic material, a liquid absorbing head (492) and an elliptical recovery air bag (493), and the retaining ring (490) is retained in the first arc-shaped clamp (48). The liquid suction rod (491) is inserted into the retaining ring (490), the liquid suction head (492) is installed at the bottom of the liquid suction rod (491), the recovery air bag (493) is installed at the top of the liquid suction rod (491), and an air inlet slit (494) is opened in the middle of the liquid suction rod (491), and the air inlet slit (494) is located above the retaining ring (490). A retaining groove (450) is opened at the bottom of one end of each air guide rod (45) away from the heating body (44), and a fixing plate (452) is installed on both side walls of each air guide rod (45), and one side wall of each fixing plate (425) is fixedly connected to the adjacent side wall of the heating body (44). The first air outlet (499) is located in the liquid suction part (49) of the first heating component (41), and the inner side wall of each retaining groove (450) is formed with a push-against inclined surface (451).

7. The systemic lupus erythematosus autoantibody detection device according to claim 6, characterized in that: An inverted T-shaped starting groove (420) is provided on the side wall of the second heating component (42). The uniform component (50) is slidably inserted into the top of the starting groove (420). The uniform component (50) comprises a shaking rod (51), a pushing rod (52), a second arc-shaped clamp (53) and a mixing tube (54). The shaking rod (51) is slidably inserted into the top of the starting groove (420). The lower end of the pushing rod (52) is fixedly connected to the top of one end of the shaking rod (51) away from the starting groove (420), and the top of the pushing rod (52) is clamped in the clamping groove (450) of the air guide rod (45). The second arc-shaped clamp (53) is fixedly connected to the end of the shaking rod (51) away from the starting groove (420). The middle part of the mixing tube (54) is opened in the second arc-shaped clamp (53).

8. The systemic lupus erythematosus autoantibody detection device according to claim 7, characterized in that: Each support assembly (60) comprises an upper support rod (61), a hinge column (62) and a lower support rod (63); the bottom of the upper support rod (61) is hinged to the side wall of the storage space (11); the hinge column (62) is rotatably mounted on the top of the upper support rod (61); the top of the lower support rod (63) is hinged to the upper cover (15); the hinge column (62) is rotatably mounted on the bottom of the lower support rod (63); the support assembly (60) adjacent to the cleaning tank (21) further comprises an upper conductive block (64), The lower conductive block (65) and the power supply (66) are arranged such that the upper conductive block (64) is fixedly mounted on the bottom of the upper support rod (61), the lower conductive block (65) is fixedly mounted on the top of the lower support rod (63), the power supply (66) is mounted on the side wall of the storage space (11), and the upper conductive block (64) and the lower conductive block (65) are both electrically connected to the power supply (66), and the heating bodies (44) of the first heating component (41), the second heating component (42) and the third heating component (43) are all electrically connected to the power supply (66).

9. The systemic lupus erythematosus autoantibody detection device according to claim 8, characterized in that: The receiving assembly (70) comprises a fixing frame (71) and a plurality of fixing boxes (72); the fixing frame (71) is fixedly mounted on the other end of the top of the fixing plate (12); and the plurality of fixing boxes (72) are fixedly mounted on the side wall of the fixing frame (71).

10. A method for detecting autoantibodies of systemic lupus erythematosus, characterized in that: Using the systemic lupus erythematosus autoantibody detection device as described in claim 9, the detection method comprises the following steps: Step S1: fixing the sample to be tested in an ELISA plate (33) of a systemic lupus erythematosus autoantibody detection device; Step S2: adding specific antibodies or antigens to allow the sample to be tested to specifically bind to the antibodies or antigens; Step S3: preparing a cleaning solution to wash the unbound substances in the detection hole (34); Step S4: adding a detection antibody to allow it to bind to the molecule to be detected to form an enzyme-labeled complex; Step S5: Wash again to remove unbound antibodies, add substrate to bind to the enzyme to produce a staining reaction, and measure the light intensity or color change of the staining product by spectroscopy to obtain the result.

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