Jujube tissue culture seedling cuttage annual breeding and rapid seedling formation, flowering and fruiting method

Abstract

The invention relates to the technical field of fruit tree cultivation, and discloses a jujube tissue culture seedling cuttage annual breeding and rapid seedling formation, flowering and fruiting method which comprises the following steps: culturing a tissue culture seedling culture medium, taking out a jujube tissue culture seedling without rooting, performing cuttage, transplanting a survival tissue culture seedling cuttage seedling, performing water and fertilizer management, performing temperature, humidity and illumination management, and applying an exogenous growth regulator. The tissue culture seedling cutting seedlings are promoted to rapidly become big seedlings and blossom and bear fruits, the big seedlings are taken for cutting to obtain second-generation tissue culture seedling cutting seedlings, and the second-generation cutting seedlings are taken for cutting to obtain third-generation tissue culture seedling cutting seedlings. According to the method, the jujube tissue culture seedlings can directly root and survive in the matrix without rooting and seedling hardening processes of a culture medium, and can quickly grow seedlings, blossom and bear fruits, so that a series of problems of strict requirements on conditions, relatively low seedling rate and the like when the traditional tissue culture seedlings enter a field for seedling hardening are avoided, and quick and efficient seedling growth is realized; the cutting seedlings of the jujube tissue culture seedlings can blossom and bear fruits in the current year, and the breeding period is shortened. The method has important significance for accelerating the jujube tree breeding process, and provides support for obtaining of clonal seedlings and rootstocks.

Description

Technical Field

[0001] The invention relates to the technical field of fruit tree cultivation, and in particular to a method for year-round propagation of jujube tissue culture seedlings by cutting and rapid seedling formation, flowering and fruiting. Background Art

[0002] Jujube is an important fruit tree native to my country and one of the five dominant economic forest tree species in my country. Jujube's reproduction methods mainly include sexual reproduction (seed reproduction) and asexual reproduction. Jujube trees obtained through seed reproduction grow robustly and have strong adaptability to the environment, but seed reproduction is prone to plant variation. Therefore, in order to maintain good character, asexual reproduction methods are often used in jujube production, including cuttings, grafting and tissue culture.

[0003] Jujube tissue culture technology has a series of problems in actual production, such as difficulty in rooting, low survival rate of transplanting, and difficulty in disinfecting explants, which restrict its actual use. Cuttings, also known as cuttings, are one of the widely used asexual propagation methods. The specific operation method is to cut the stems, leaves, roots, buds and other organs of the plant, soak them in plant growth regulators (auxins), make them take root and plant them, and become independent new plants. However, there are problems such as low seedling rate and long cycle from tissue culture seedlings to flowering and fruiting, which affect the breeding process of jujube trees and the efficiency of obtaining asexual seedlings and rootstocks.

[0004] Therefore, it is necessary to provide a method for the year-round propagation of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting. The method utilizes jujube cutting technology to establish a technical system from tissue culture seedling cutting rooting to rapid seedling formation and then to tissue culture seedling flowering and fruiting, which is of great significance for improving production efficiency, cultivating asexual seedlings and early high-quality stable asexual rootstocks. Summary of the invention

[0005] The invention aims to provide a method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling growth, flowering and fruiting. The method cultivates complete jujube tree seedlings by cuttings of unrooted jujube tissue culture seedlings obtained in a culture medium, so that rapid and efficient growth of large seedlings can be achieved, flowering and fruiting can be obtained in the same year, the breeding process of asexual large seedlings can be accelerated, and a large number of asexual seedlings and rootstocks with stable and high-quality traits can be obtained.

[0006] The technical method adopted by the present invention is as follows:

[0007] The present invention provides a method for year-round propagation of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting, comprising the following steps:

[0008] (1) The jujube tissue culture seedlings Y①-1 type after 45 days of culture in MS medium were used as cutting materials. Later, the types of jujube tissue culture seedlings were expanded and 35 types of jujube tissue culture seedlings were added as cutting materials. The tissue culture seedlings with uniform size and strong growth in the culture medium were selected as cuttings.

[0009] (2) Take out the tissue culture seedlings from the culture medium, remove the proliferative part at the base and the bottom leaves, leaving about 0.5 cm at the bottom and 3-4 leaves at the top. The overall length of the cuttings is 4-5 cm.

[0010] (3) Soak the treated tissue culture seedling cuttings in a 1.25 g / L carbendazim solution for 5 min, dip them in a 1000 mg / L IBA solution for 10 s, insert the lower end of the cuttings into the prepared cutting medium, and place them in a light incubator at 25-30°C, 95%, 6000 lx for rooting.

[0011] (4) When the above-mentioned tissue culture seedlings take root, the roots break through the plug tray, and the above-ground part begins to grow, the jujube tissue culture seedlings of basically the same size and strong growth are transplanted into a seedling pot with a diameter of 25 cm. The seedling medium: earthworm castings: peat: perlite = 3:2:1:1 is selected as the medium for large seedling culture. Water and fertilizer are fully supplied. CGMP is sprayed every 7 days, and 1000 times liquid starch-dissolving microbial fertilizer is applied every 10 days, and 0.88 g / L Hoagland nutrient solution is applied every 30 days.

[0012] (5) The tissue culture seedlings and cuttings began to grow rapidly 20 days after transplanting, and began to branch in large numbers 30 days later. The average height of the cuttings was 15 cm, which was defined as the first generation of tissue culture seedlings and cuttings.

[0013] (6) When the first generation of tissue culture seedlings (without pinching) grow to an average plant height of 46 cm and an average stem diameter of 3.13 mm, flower buds begin to appear on the cuttings; when the first generation of tissue culture seedlings (with pinching) grow to an average plant height of 21 cm and an average stem diameter of 2.67 mm, flower buds begin to appear on the cuttings, spray potassium dihydrogen phosphate solution on the leaves every 15 days, apply 1000 times liquid starch-dissolving microbial fertilizer once every 10 days, and spray 30 mg / L gibberellin solution once every 7 days to promote their extensive flowering and fruit setting.

[0014] (7) Cuttings from the first-generation tissue culture seedling cuttings to obtain second-generation tissue culture seedling cuttings.

[0015] (8) Cuttings from the second-generation tissue culture seedlings as tender shoots, and use them as cuttings to obtain third-generation tissue culture seedlings.

[0016] Furthermore, in step (1), the jujube tissue culture seedlings are cultured in MS culture medium at an environmental temperature of 20° C., a humidity of 45%, and a light intensity of 3000 lx.

[0017] Furthermore, in the step (2), the proliferating part of the tissue culture seedlings after growing in the MS medium for 45 days needs to be cut off before cutting.

[0018] Furthermore, in step (3), the cutting substrate is peat:perlite=3:1, and the cutting substrate needs to be disinfected with 1.25g / L carbendazim solution according to the weight of the substrate before use (substrate:carbendazim solution=1100g:1L).

[0019] Furthermore, in step (3), the base of the cuttings should be reduced from breaking during the cutting process, and after the cutting is completed, the cuttings should be kept perpendicular to the substrate to reduce lodging, thereby increasing the survival rate of the cuttings and ensuring the normal upward growth of the later transplanted cuttings.

[0020] Furthermore, the transplanting of cuttings to large seedlings in steps (4) and (5) is carried out in an artificial climate control room, with environmental conditions of 25-30°C during the day and 20-25°C at night, a light intensity of 6000lx, and a photoperiod of 16h / 8h.

[0021] Furthermore, in step (6), the top buds of the jujube seedlings are removed by pinching the top of the jujube seedlings. After the flower buds appear, the cutting seedlings are moved into an artificial climate control room with an environmental condition of 23-25°C during the day, 16-18°C at night, a light intensity of 20,000 lx, and a photoperiod of 16h / 8h.

[0022] Furthermore, in the step (7), a robust and uniformly growing cutting seedling is selected from the first generation tissue culture seedlings, and the tender shoots of the cutting seedlings are cut at the same position for cutting. The cuttings are cut with a length of 8-10 cm, 15-20 leaves and 4-5 cm, 5-7 leaves, and are classified for cutting. The cuttings with a length of 8-10 cm are cut from the middle and divided into two sections for cutting respectively, with no tip at the lower end and a tip at the upper end, and are marked respectively. After trimming, the lower end of the cutting retains 1-2 cm, the upper end retains 4-5 leaves, and the overall length is 4-5 cm. The cutting method and the culture method are the same as those of the first generation cutting seedlings. The culture method after survival is the same as that of the first generation.

[0023] Furthermore, the second generation of cuttings in step (8) is obtained from the first generation of cuttings, so the morphology after survival is similar to that of the jujube hanging, and the third generation of cuttings is derived from the second generation of jujube hanging, and its processing method and culture method are the same as those of the second generation of cuttings. The culture method after survival is the same as that of the first generation.

[0024] Compared with the related art, the method for annual propagation of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting provided by the present invention has the following beneficial effects:

[0025] The present invention utilizes unrooted jujube tissue culture seedlings for cuttings, rooting, transplanting, water and fertilizer management, temperature, light and humidity management, application of exogenous growth regulators and other measures, so as to achieve rapid seedling formation after direct cutting and rooting of tissue culture seedlings without rooting, rapid breeding throughout the year and flowering and fruiting. The invention solves the problems of low survival rate of transplanting and training of jujube tissue culture seedlings after rooting, late flowering of seedlings, etc., accelerates the breeding process of clone seedlings, obtains a large number of clone seedlings and rootstocks with stable and high-quality traits, and improves production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Photos of the process of inoculating the tissue culture seedlings into the culture medium provided in 1, the 'jujube tissue culture seedlings' grown in the culture medium for 30 days, and the 'jujube tissue culture seedlings' taken out of the culture medium without being trimmed and ready for cutting;

[0027] Figure 2 The photos of the tissue culture seedlings provided in Example 1 after pruning and ready for cuttings, and the photos of the jujube tissue culture seedlings after cuttings and rooting;

[0028] Figure 3 The photos of the 'jujube tissue culture seedlings' provided in Example 1 growing in the cutting medium for 30 days and transplanting to the seedling medium;

[0029] Figure 4 This is a photo of the 'jujube tissue culture seedlings' provided in Example 1 growing in the transplanting medium to an average plant height of 15 cm;

[0030] Figure 5 The flowering photos of the 'jujube tissue culture seedlings' that were pinched after 50 days of transplantation and the 'jujube tissue culture seedlings' that were not pinched after 90 days of transplantation provided in Example 1;

[0031] Figure 6 The result photo of the 'jujube tissue culture seedlings' treated with pinching provided in Example 1;

[0032] Figure 7 The photos of the second generation tissue culture seedlings provided in Example 1, where the jujube hangings were picked from the 'jujube tissue culture seedlings' with an average plant height of 15 cm and the tips were retained and pinched;

[0033] Figure 8 The photos of the third generation tissue culture seedlings and cuttings provided for Example 1. DETAILED DESCRIPTION

[0034] The present invention will be further described below in conjunction with the accompanying drawings and implementation modes.

[0035] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0036] Embodiment 1 A technology for annual propagation of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting, comprises the following steps:

[0037] 1. Culture medium for jujube tissue culture seedlings

[0038] (1) On February 29, 2024, various types of jujube tissue culture seedlings were inoculated into MS culture medium and cultured in the tissue culture room of the East Campus of Hebei Agricultural University at an ambient temperature of 22-25°C and a light intensity of 3000 lx for 45 days.

[0039] (2) On April 8, 2024, the jujube tissue culture seedlings in the culture medium were taken out, the bottom hyperplastic part and the leaves were removed, and the cuttings with an overall length of 4-5 cm were obtained. They were placed in a 1.25 g / L carbendazim solution and soaked for 5 min. They were taken out and dried or wiped dry. The base of the cuttings was soaked in a 1000 mg / LIBA solution for 10 s. After taking them out, they were inserted into a 32-hole plug tray pre-filled with cutting medium. The cutting medium had been disinfected with a 1.25 g / L carbendazim solution for 5-20 h. After the cuttings were completed, they were watered thoroughly, with water dripping from the bottom of the plug tray as the standard for watering the medium thoroughly. They were then cultured in a light incubator under the following environmental conditions: daytime temperature: 30 °C, nighttime temperature: 25 °C, light intensity: 6000 lx, and photoperiod: 16 h / 8 h. The survival rate was 65.25%.

[0040] (3) When the above-mentioned tissue culture seedlings take root, the root system breaks through the plug tray, and the aboveground part begins to grow, the jujube tissue culture seedlings of basically the same size and strong growth are transplanted into a seedling pot with a diameter of 25CM, and the seedling medium: earthworm castings: peat: perlite = 3:2:1:1 is selected as the large seedling culture medium, and water and fertilizer are fully supplied. 9.18mg / LCGMP is sprayed every 7 days, 1000 times liquid starch-dissolving microbial fertilizer is poured once every 10 days, and 0.88g / L Hogland nutrient solution is poured once every 30 days. The cultivation is carried out in an artificial climate control room, and the environmental conditions are 28℃ during the day, 23℃ at night, 6000lx of light intensity, and 16h / 8h of photoperiod. The tissue culture seedlings and cuttings began to grow rapidly 20 days after transplanting, and began to branch in large numbers 30 days after transplanting. The average height of the cuttings was 15 cm, the average stem diameter was 1.49 mm, the average length of the jujube branches was 5 cm, and the average number of secondary branches was 6. They were defined as the first generation of tissue culture seedlings and cuttings. (50 days after transplanting) when the first generation of tissue culture seedlings and cuttings grew to an average height of 21 cm and an average stem diameter of 2.63 mm, they were topped and ring-cut to reduce the downward transport of nutrients and promote flower bud differentiation; (90 days after transplanting) when the first generation of tissue culture seedlings and cuttings (not topped or ring-cut) grew to an average plant height of 46 cm, an average stem diameter of 3.13 mm, and an average length of jujube branches of 38.5 cm, flower buds began to appear in the cuttings. For the tissue culture seedlings and cuttings treated with the above two types of treatments, potassium dihydrogen phosphate solution was sprayed on the leaves every 15 days, 1000 times liquid starch-dissolving microbial fertilizer was applied once every 10 days, and 30 mg / L gibberellin solution was sprayed once every 7 days to promote their extensive flowering and fruit setting.

[0041] (4) When the tissue culture seedlings grow to an average plant height of 15 cm and begin to branch, the second generation of tissue culture seedlings can be cut and propagated. Select tender branches of the same position as the tissue culture seedlings. The average length of the branches is 4-5 cm and the number of leaves is 5-6. The branches are pruned. One type of branches retains the tip and the other type removes the tip. Both types remove 2-3 leaves from the lower end and retain 4-5 leaves on the upper part of the 1-2 cm stem segment. Insert the cutting medium and place it in a light incubator for culture. The culture conditions are 32°C during the day, 25°C at night, and 10% light. The light intensity is 15000lx, and the photoperiod is 16h / 8h. On August 1, 2024 (30 days after the second-generation cuttings), the survival rate of the jujube hangings was 100%, the average plant height growth of the aboveground part was 0.013cm, and the average growth of the aboveground stem diameter was 0mm. The survival rate of the jujube hangings with removed tips was 87.5%, the average plant height growth of the aboveground part was 0cm, and the average growth of the aboveground stem diameter was 0mm. The growth of the underground roots was the main process of survival of the cuttings. Rooting began 14 days after cutting, and the roots broke through the seedling pot 30 days after cutting.

[0042] (5) On September 14, 2024, the second-generation tissue culture seedlings were used for cuttings. After 30 days, the third-generation tissue culture seedlings were obtained, and the survival rate was 100%.

[0043] (6) The second and third generation tissue culture seedlings are rapidly cultured into large seedlings, and the culture conditions after transplanting are the same as those for the first generation.

[0044] 2. Cultivation effect identification

[0045] 1. Identification of the survival rate of cuttings: In order to investigate the survival of tissue culture seedlings, the survival rates of the three generations of tissue culture seedlings in the above embodiment were observed and counted, and the survival rates of cuttings were calculated. The results are shown in Table 1.

[0046] Table 1 Statistics of the survival rate of cuttings of the third generation of cuttings

[0047]

[0048] 2. Identification of root growth of cuttings: In order to investigate the root growth of the third-generation tissue culture seedlings, the root systems of the third-generation tissue culture seedlings in the above embodiment were observed and counted to obtain the root conditions of the third-generation tissue culture seedlings. The results are shown in Table 2.

[0049] Table 2 Investigation on root growth of third-generation tissue culture seedlings and cuttings

[0050]

[0051] 3. Identification of the rapid cultivation effect of large seedlings: In order to investigate the rapid seedling formation of the first-generation tissue culture seedlings and cuttings, the average values ​​of plant height, stem diameter, secondary branch and jujube hanging length of the first-generation tissue culture seedlings at different stages in the above embodiment were statistically analyzed, and the plant height, stem diameter and jujube hanging growth of the tissue culture seedlings at different stages were calculated. The results are shown in Tables 3 and 4.

[0052] Table 3 Investigation on plant height, stem thickness and secondary branch characteristics of first generation cutting seedlings

[0053]

[0054]

[0055] Table 4 Survey on plant height, stem diameter and growth of first generation cutting seedlings

[0056] Processing time 7d 14d 21d Plant height growth (cm) 1.57 0.92 0.77 Stem diameter growth (mm) 0.06 0.03 0.07 Growth of jujube hanging length (cm) 0.85 0.22 1.04

[0057] 4. Identification of rapid flowering and fruiting traits of large seedlings: In order to investigate the rapid flowering of large seedlings of the first generation of tissue culture seedlings and cuttings, the earliest flowering time, average number of flowers, initial flowering node, fruiting node, plant height, stem thickness, and child length of the untopped large seedlings and topped large seedlings in the above embodiments were statistically analyzed. The results are shown in Table 5.

[0058] Table 5. Investigation of plant traits of large seedlings flowering and fruiting

[0059]

[0060] The above results show that the present invention can make the jujube tissue culture seedlings directly take root and survive in the plug tray containing the substrate without the rooting and training process, avoiding a series of problems such as difficulty in training and transplanting of tissue culture seedlings obtained under traditional tissue culture conditions, and low survival rate, thereby achieving rapid and efficient seedling formation. The use of various types of jujube tissue culture seedlings improves the applicability of the present invention, and the present invention can make the jujube tissue culture seedlings quickly grow into seedlings and bear fruit in the same year. The invention is of great significance for accelerating the breeding process of jujube trees, and a large number of clone seedlings and trait-stable rootstocks can be quickly obtained.

[0061] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for annual propagation of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting, characterized in that: The steps include: Step 1, placing the 1-2 cm tissue culture seedling stem segment in MS culture medium and culturing it for about 45 days, in the unrooted state, the tissue culture seedling grows to 4-6 cm, 6-8 leaves, and selecting the unrooted jujube tissue culture seedlings of uniform size and strong growth as cutting materials; Step 2, taking out the jujube tissue culture seedlings from the culture medium, removing the callus tissue proliferating at the base of the tissue culture seedlings and the withered leaves at the bottom, retaining the upper 3-5 leaves, and the length of the whole tissue culture seedling cuttings is 4-5 cm, with 3-5 leaves at the top and a 0.5 cm stem segment at the bottom; Step 3, soak the treated tissue culture seedling cuttings in a 1.25g / L carbendazim solution for 5 minutes, dip them in a 1000mg / L IBA solution for 10 seconds, insert the lower end of the cuttings into the prepared cutting medium, and place them in a light incubator at 25-30°C, 95%, 6000lx for rooting; Step 4, when the above-mentioned tissue culture seedlings take root, the root system breaks through the plug tray, and the aboveground part begins to grow, the jujube tissue culture seedlings of basically the same size and strong growth are transplanted into a seedling pot with a diameter of 25CM, and the seedling medium: earthworm castings: peat: perlite = 3:2:1:1 is selected as the large seedling culture medium, and water and fertilizer are fully supplied. 9.18mg / L CGMP is sprayed every 7d, 1000 times liquid starch-dissolving microbial fertilizer is poured once every 10d, and 0.88g / L Hogland nutrient solution is poured once every 30d; Step 5, the tissue culture seedlings and cuttings began to grow rapidly 20 days after transplanting, and began to branch in large numbers after 30 days. The average plant height of the cuttings was 15 cm, the average stem diameter was 1.49 mm, the average number of secondary branches was 3, and the length of the jujube hanging was 4-5 cm, which was defined as the first generation tissue culture seedlings and cuttings; Step 6, when the first-generation tissue culture seedlings without pinching grow to an average plant height of 46cm and an average stem thickness of 3.13mm, flower buds begin to appear on the cuttings; the first-generation tissue culture seedlings with pinching grow to an average plant height of 21cm and an average stem thickness of 2.67mm, and flower buds begin to appear on the cuttings, and potassium dihydrogen phosphate solution is sprayed on its leaf surface every 15d, 1000 times liquid starch-dissolving microbial fertilizer is applied once every 10d, and a gibberellin solution with a concentration of 30mg / L is sprayed once every 7d to promote its large-scale flowering and fruit setting; Step 7, cutting the tender branches from the first-generation tissue culture seedling cuttings as cuttings, and performing cuttings to obtain the second-generation tissue culture seedling cuttings; Step 8, cutting off the tender shoots of jujube from the second-generation tissue culture seedling cuttings as cuttings, and performing cuttings to obtain the third-generation tissue culture seedling cuttings; After cutting the tender shoots of the jujube hanging from the first generation tissue culture seedlings and the second generation tissue culture seedlings, they can be cultured for 15-30 days according to step 4 of the tissue culture seedling cultivation method, and then the tender shoots of the jujube hanging can be cut again for cutting.

2. The method for annual breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In the step 1, the jujube tissue culture seedlings are cultured in MS culture medium at an ambient temperature of 20° C., a humidity of 45%, and a light intensity of 3000 lx.

3. The method for annual breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In step 2, the proliferative part of the tissue culture seedlings grown in the MS medium for 45 days needs to be cut off before cutting.

4. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In the step 3, the cutting substrate is peat:perlite=3:1, and the cutting substrate needs to be disinfected with 1.25g / L carbendazim solution according to the weight of the substrate before use (substrate:carbendazim solution=1100g:1L).

5. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In the step 3, the cutting base should be reduced from breaking during the cutting process, and after the cutting is completed, the cuttings of the cuttings are ensured to be vertical to the substrate, and lodging is reduced to improve the cutting survival rate and ensure the normal upward growth of the later transplanted cuttings.

6. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: The transplanting of cutting seedlings into large seedlings in step 4 and step 5 is carried out in an artificial climate control room, and the environmental conditions are 25-30° C. during the day, 20-25° C. at night, the light intensity is 6000 lx, and the photoperiod is 16h / 8h.

7. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In step 6, the jujube tissue culture seedlings are pinched to remove the terminal buds of the jujube seedlings; the pinched and unpinched tissue culture seedlings are moved into an artificial climate control room with an environmental condition of 23-25°C during the day, 16-18°C at night, a light intensity of 20,000 lx, and a photoperiod of 16h / 8h.

8. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In the step 7, robust and uniformly growing cutting seedlings are selected from the first generation tissue culture seedlings, and tender branches of the cutting seedlings are cut at the same position for cutting. The cuttings are 8-10 cm in length, 15-20 leaves and 4-5 cm in length, 5-7 leaves, and are classified for cutting. The jujube branches with a length of 8-10 cm are cut from the middle and divided into two sections for cutting respectively, with a sharp tip at the upper end and a sharp tip at the lower end, and are marked respectively. After trimming, the lower end of the jujube cuttings retains 1-2 cm, the upper end retains 4-5 leaves, and the overall length is 4-5 cm; the cutting method and the cultivation method are the same as those of the first generation cutting seedlings; the cultivation method after survival is the same as that of the first generation.

9. The method for year-round breeding of jujube tissue culture seedlings by cuttings and rapid seedling formation, flowering and fruiting as claimed in claim 1, characterized in that: In step 8, the second generation cuttings seedlings are obtained from the first generation cuttings seedlings, so their morphology after survival is similar to that of the jujube hangings. The third generation cuttings seedlings are derived from the second generation jujube hangings, and their processing and cultivation methods are the same as those of the second generation cuttings seedlings; the cultivation method after survival is the same as that of the first generation.

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