Biological sample freezing device
By designing the coordination of the layered frozen shelf structure and bearing cavity, the problem of space waste and inconvenience caused by the frozen shelf height is solved, and the convenient extraction and rotation stability of the frozen shelf is achieved.
Patent Information
- Application Number
- CN202411831795.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-12
- Publication Date
- 2025-04-04
AI Technical Summary
In the existing biological sample freezing device, the height of the frozen storage shelf is high, resulting in a higher height need to be reserved above the liquid nitrogen tank, which wastes space and is inconvenient to use.
A layered frozen storage shelf structure is designed, and the outer shaft and the inner shaft can achieve separate rotation of the upper and lower multi-layer frozen storage shelf to avoid interference and jamming.
It effectively reduces the high demand above the liquid nitrogen tank, saves space, and improves the extraction convenience of the frozen storage rack, avoiding the problem of stuck during rotation.
Smart Images
Figure CN119744846A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of biological sample cryopreservation, and in particular to a biological sample cryopreservation device. Background Art
[0002] The statements herein merely provide background information related to the present application and do not necessarily constitute prior art.
[0003] With the rapid development of the biopharmaceutical industry, large-scale biological sample banks have emerged, and the storage volume of biological samples has become larger and larger. Generally, biological samples such as tissues and cells in clinical or laboratory environments are placed in cryopreservation racks in liquid nitrogen tanks for storage.
[0004] When taking and placing biological samples, the freezing rack needs to be lifted up first, and then the biological samples can be taken and placed. However, the freezing rack is relatively high, so a relatively high height needs to be reserved above the liquid nitrogen tank, which not only wastes space but also is inconvenient to use.
[0005] Therefore, there are layered freezing racks in the related art, that is, the original freezing rack is changed into two or more freezing racks distributed up and down, for example, including a first freezing rack located at the top and a second freezing rack located at the bottom. Then there is no need to reserve a higher height above the liquid nitrogen tank, which will not waste space and is convenient to use. For example, the original freezing rack has a height of A, and the height that needs to be reserved above the liquid nitrogen tank is A. The layered freezing racks in the related art are equivalent to breaking the freezing rack with a height of A into 2 or more freezing racks. Taking 2 freezing racks of equal height as an example, the heights of the two freezing racks are both A / 2, and the height that needs to be reserved above the liquid nitrogen tank is A / 2.
[0006] In the layered freezing racks, the multiple freezing racks on the top are convenient to extract, and can be directly extracted through the opening on the top. In order to facilitate the extraction of the multiple freezing racks below, a channel can be set at the upper freezing rack for the extraction of the freezing rack below. In order to facilitate the extraction of any of the multiple freezing racks on the top and the multiple freezing racks on the bottom, the multiple freezing racks on the top and the multiple freezing racks on the bottom need to be able to rotate separately (so that any of the upper freezing racks can correspond to the position of the top opening, and any of the lower freezing racks can correspond to the position of the top opening and the channel).
[0007] The multiple freezing racks on the upper side and the multiple freezing racks on the lower side can be rotated separately by means of inner and outer shafts. However, this method easily causes interference between the multiple freezing racks on the upper side and the multiple freezing racks on the lower side, thereby causing jamming during rotation. Summary of the invention
[0008] In view of the above problems, the present application is proposed to provide a biological sample freezing device that overcomes the above problems or at least partially solves the above problems.
[0009] The present application provides a biological sample freezing device, comprising: a liquid nitrogen tank, the liquid nitrogen tank defining a receiving chamber, the top of the receiving chamber having an inlet and outlet; an outer shaft, the outer shaft extending in a vertical direction, the outer shaft defining an axial cavity, the outer shaft at least partially disposed in the receiving chamber, the portion of the outer shaft located in the receiving chamber being fixedly connected to a plurality of first freezing racks, the first freezing racks being used to store biological samples, the first freezing racks entering and exiting the receiving chamber through the inlet and outlet; at least one bearing seat, the bearing seat being fixedly connected to a first end or a second end of the outer shaft, the bearing seat defining a bearing cavity, a bearing being disposed in the bearing cavity with upper and lower limit positions, the outer ring of the bearing being fixedly connected to the bearing seat; an inner shaft, the inner shaft passing through the axial cavity and at least partially disposed below the outer shaft, the inner shaft being fixedly connected to the inner ring of the bearing, the portion of the inner shaft located below the outer shaft being fixedly connected to a plurality of second freezing racks, the second freezing racks also being used to store biological samples, the region corresponding to the first freezing rack being provided with a channel for the second freezing racks to pass through, The second freezing rack enters and exits the accommodating chamber through the inlet and outlet and the passage; the first freezing rack and the second freezing rack both define a plurality of vertically arranged rack positions, the rack positions are used to place a freezing box, the freezing box is used to place a freezing tube, and the freezing tube is used to store a biological sample; the top of the accommodating chamber has an axial hole, the first end of the outer shaft is arranged in the accommodating chamber, and the second end of the outer shaft passes through the axial hole and is arranged outside the accommodating chamber; the at least one bearing seat includes a first bearing seat, the first bearing seat is fixedly connected to the first end of the outer shaft, the first bearing seat defines a first bearing cavity, a first bearing is arranged in the first bearing cavity, and the outer ring of the first bearing is fixedly connected to the first bearing seat; a first flange is fixed to the middle part of the outer shaft, the first bearing seat is detachably fixedly connected to the first end of the outer shaft by a thread, the lower end of the first flange and the upper end of the first bearing seat clamp and fix a first frame, and the first flange is fixedly connected to the first frame, and the first frame is used to place a plurality of the first freezing racks.
[0010] Optionally, the at least one bearing seat also includes a second bearing seat, which is fixedly connected to the second end of the outer shaft, and the second bearing seat defines a second bearing cavity, in which a second bearing is arranged, and the outer ring of the second bearing is fixedly connected to the second bearing seat.
[0011] Optionally, a first driving wheel is fixed to the upper end of the second bearing seat, and the first driving wheel is connected to a first driving mechanism to drive the outer shaft to rotate.
[0012] Optionally, a third bearing is provided at the connection between the outer shaft and the shaft hole, an inner ring of the third bearing is fixed to the outer shaft, and an outer ring of the third bearing is fixed to the liquid nitrogen tank.
[0013] Optionally, the first end of the inner shaft is located in the accommodating cavity, and the second end of the inner shaft passes through the shaft cavity and the shaft hole is located outside the accommodating cavity.
[0014] Optionally, a second flange is fixed to the portion of the inner shaft below the outer shaft, a fixing piece is detachably fixedly connected to the first end of the inner shaft, a second frame is clamped and fixedly secured by the lower end of the second flange and the upper end of the fixing piece, and the second flange is fixedly connected to the second frame, and the second frame is used to place a plurality of the second freezing racks.
[0015] Optionally, a second driving wheel is fixed to the second end of the inner shaft, and the second driving wheel is connected to a second driving mechanism to drive the inner shaft to rotate.
[0016] The biological sample freezing device provided in the present application is provided with a bearing cavity and a bearing in the bearing cavity, thereby ensuring the coaxiality of the outer shaft and the inner shaft, avoiding tilting between the first freezing rack and the second freezing rack, thereby avoiding interference and avoiding jamming during rotation. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Other objects and advantages of the present application will be apparent from the following description of the present application with reference to the accompanying drawings, which will help to provide a comprehensive understanding of the present application.
[0018] Figure 1 is a schematic structural diagram of a biological sample freezing device according to some embodiments of the present application; Figure 2 is a schematic diagram of the structure of a biological sample freezing device according to some embodiments of the present application without the liquid nitrogen tank; Figure 3 yes Figure 2 The structure shown is a schematic diagram of the structure after the first frame and the second frame are hidden; Figure 4 yes Figure 3 A schematic enlarged view of region A is shown; Figure 5 yes Figure 3 The structure shown is a schematic diagram of the structure without the first driving wheel and the second driving wheel; Figure 6 yes Figure 5 A schematic enlarged view of region B is shown; Figure 7 yes Figure 5 The structure shown is a schematic diagram of the structure after the inner shaft and the third bearing are hidden; Figure 8 yes Figure 7 Another view of the structure shown.
[0019] In the figure, 10 is a biological sample freezing device, 100 is a liquid nitrogen tank, 110 is a accommodating cavity, 111 is an inlet and outlet, 200 is an outer shaft, 220 is a first flange, 300 is a first frame, 310 is a first freezing rack, 320 is a channel, 410 is a first bearing seat, 411 is a first bearing cavity, 412 is a first bearing, 420 is a second bearing seat, 421 is a second bearing cavity, 422 is a second bearing, 500 is an inner shaft, 510 is a second flange, 520 is a fixing member, 600 is a second frame, 700 is a first driving wheel, 800 is a third bearing, and 900 is a second driving wheel. DETAILED DESCRIPTION
[0020] In order to make the purpose, technical solution and advantages of the present application clearer, the technical solution of the present application will be clearly and completely described below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiment is one embodiment of the present application, not all embodiments. Based on the described embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.
[0021] Unless otherwise defined, technical or scientific terms used in this application should have the common meanings understood by persons having ordinary skills in the field to which this application belongs.
[0022] The present application provides a biological sample freezing device 10, Figure 1 is a schematic structural diagram of a biological sample freezing device 10 according to some embodiments of the present application. Figure 2 is a schematic structural diagram of a biological sample freezing device 10 according to some embodiments of the present application without the liquid nitrogen tank 100, Figure 3 yes Figure 2 The structure shown is a schematic diagram of the structure after the first frame 300 and the second frame 600 are hidden. Figure 4 yes Figure 3 A schematic enlarged view of region A is shown, Figure 5 yes Figure 3 The structure shown is a schematic diagram of the structure after the first driving wheel 700 and the second driving wheel 900 are hidden. Figure 6 yes Figure 5 A schematic enlarged view of region B is shown, Figure 7 yes Figure 5 The structure shown is a schematic diagram of the structure after the inner shaft 500 and the third bearing 800 are hidden. Figure 8 yes Figure 7 Another view of the structure shown.
[0023] The biological sample freezing device 10 includes a liquid nitrogen tank 100, an outer shaft 200, at least one bearing seat and an inner shaft 500. The liquid nitrogen tank 100 defines a receiving chamber 110, and the top of the receiving chamber 110 has an inlet and outlet 111. The outer shaft 200 extends in the vertical direction, and the outer shaft 200 defines an axial cavity. The outer shaft 200 is at least partially disposed in the receiving chamber 110. The portion of the outer shaft 200 located in the receiving chamber 110 is fixedly connected to a plurality of first freezing racks 310, and the first freezing racks 310 are used to store biological samples. The first freezing racks 310 enter and exit the receiving chamber 110 through the inlet and outlet 111. The bearing seat is fixedly connected to the first end or the second end of the outer shaft 200, and the bearing seat defines a bearing cavity. A bearing is disposed in the bearing cavity with upper and lower limit positions, and the outer ring of the bearing is fixedly connected to the bearing seat. The inner shaft 500 passes through the shaft cavity and is at least partially located below the outer shaft 200. The inner shaft 500 is fixedly connected to the inner ring of the bearing. The portion of the inner shaft 500 located below the outer shaft 200 is fixedly connected to a plurality of second freezing racks, which are also used to store biological samples. The area corresponding to the first freezing rack 310 is provided with a channel 320 for the second freezing rack to pass through. The second freezing rack enters and exits the accommodating chamber 110 through the inlet and outlet 111 and the channel 320. The first freezing rack 310 and the second freezing rack both define a plurality of rack positions arranged vertically, which are used to place freezing boxes, which are used to place freezing tubes, and which are used to store biological samples. The top of the accommodating cavity 110 has an axial hole, the first end of the outer shaft 200 is arranged in the accommodating cavity 110, and the second end of the outer shaft 200 is arranged outside the accommodating cavity 110 through the axial hole (thereby, it is convenient for users to operate the outer shaft 200 externally, including adjusting the angle, coaxiality, etc. when a fault occurs). The at least one bearing seat includes a first bearing seat 410, the first bearing seat 410 is fixedly connected to the first end of the outer shaft 200, the first bearing seat 410 defines a first bearing cavity 411, a first bearing 412 is arranged in the first bearing cavity 411, and the outer ring of the first bearing 412 is fixedly connected to the first bearing seat 410 (it is convenient for users to operate the second ends of the outer shaft 200 and the inner shaft 500 externally, so that the first end is inconvenient to adjust, therefore, the first bearing seat 410 is arranged at the first end). A first flange 220 is fixed to the middle of the outer shaft 200, the first bearing seat 410 is detachably fixedly connected to the first end of the outer shaft 200, the lower end of the first flange 220 and the upper end of the first bearing seat 410 clamp and fix the first frame 300, and the first flange 220 is fixedly connected to the first frame 300, and the first frame 300 is used to place multiple first freezing racks 310.
[0024] The biological sample freezing device 10 provided in the present application is provided with a bearing cavity and a bearing in the bearing cavity, thereby ensuring the coaxiality of the outer shaft 200 and the inner shaft 500, avoiding tilting between the first freezing rack 310 and the second freezing rack, thereby avoiding interference and preventing jamming during rotation.
[0025] The biological sample freezing device 10 may also be provided with a rack lifting mechanism, which is used to extract the first freezing rack 310 and the second freezing rack. Specifically, the rack lifting mechanism extracts the first freezing rack 310 through the inlet and outlet 111, and extracts the second freezing rack through the channel 320 and the inlet and outlet 111.
[0026] The bearing seat can be fixedly connected to the first end or the second end of the outer shaft 200 by threaded fit, the outer ring of the bearing can be fixedly connected to the bearing seat by interference fit, and the inner shaft 500 can be fixedly connected to the inner ring of the bearing by interference fit.
[0027] Compared with the method of setting bearings between shafts (in a deep low temperature environment, the gap between the bearing and the inner shaft and the outer shaft will become larger, causing the bearing to loosen and shift), the present application uses a bearing seat to maintain the coaxiality between the inner shaft and the outer shaft, thereby avoiding the up and down movement of the bearing and reducing the impact of the deep low temperature environment on the stability of the bearing's coaxiality effect.
[0028] The first bearing seat 410 will bear part of the pressure of the first freezing rack 310, directly or indirectly conflicting with the first freezing rack 310, and the first freezing rack 310 is fixed to the outer shaft 200 through the first flange 220, that is, the first freezing rack 310 will rotate together with the outer shaft 200, and there is a direct or indirect friction force between the first freezing rack 310 and the first bearing seat 410, which can play a role in promoting the first bearing seat 410 to follow the rotation, reduce the occurrence of thread loosening, ensure the connection stability between the threaded bearing seat and the shaft, and share the pressure of the first flange 220 to avoid deformation and damage of the first flange 220.
[0029] In some embodiments, the at least one bearing seat further includes a second bearing seat 420, the second bearing seat 420 is fixedly connected to the second end of the outer shaft 200, the second bearing seat 420 defines a second bearing cavity 421, a second bearing 422 is disposed in the second bearing cavity 421, and the outer ring of the second bearing 422 is fixedly connected to the second bearing seat 420. Thus, the coaxiality of the inner shaft 500 and the outer shaft 200 is further ensured.
[0030] In some embodiments, the first driving wheel 700 is fixed to the upper end of the second bearing seat 420, and the first driving wheel 700 is connected to the first driving mechanism to drive the outer shaft 200 to rotate. Therefore, the first driving mechanism can be arranged outside the accommodating chamber 110 of the liquid nitrogen tank 100 to avoid the low temperature environment from affecting the first driving mechanism.
[0031] In some embodiments, a third bearing 800 is provided at the connection between the outer shaft 200 and the shaft hole, the inner ring of the third bearing 800 is fixed to the outer shaft 200, and the outer ring of the third bearing 800 is fixed to the cavity. Specifically, the outer ring of the third bearing 800 can be fixed to the cavity through a third flange, that is, the opening of the third bearing 800 and the third flange is interference fit, and the third flange and the cavity can be threaded. In this way, wear of the outer shaft 200 and the cavity at the connection is avoided.
[0032] In some embodiments, the first end of the inner shaft 500 is located in the accommodating cavity 110, and the second end of the inner shaft 500 passes through the shaft cavity and the shaft hole is located outside the accommodating cavity 110. Therefore, when a fault occurs, it is convenient for the user to operate the inner shaft 500 externally, including adjusting the angle, coaxiality, etc.
[0033] In some embodiments, the portion of the inner shaft 500 below the outer shaft 200 is fixed with a second flange 510, the first end of the inner shaft 500 is detachably fixedly connected with a fixing member 520, the lower end of the second flange 510 and the upper end of the fixing member 520 clamp and fix the second frame 600, and the second flange 510 is fixedly connected to the second frame 600, and the second frame 600 is used to place a plurality of the second freezing racks. Thus, the fixing effect of the second frame 600 is ensured.
[0034] In some embodiments, the second end of the inner shaft 500 is fixed with a second driving wheel 900, and the second driving wheel 900 is connected to a second driving mechanism to drive the inner shaft 500 to rotate. Thus, the second driving mechanism can be arranged outside the accommodating chamber 110 of the liquid nitrogen tank 100 to avoid the low temperature environment from affecting the second driving mechanism.
[0035] Regarding the embodiments of the present application, it should also be noted that, in the absence of conflict, the embodiments of the present application and the features therein can be combined with each other to obtain new embodiments.
[0036] The above are only specific implementation methods of the present application, but the protection scope of the present application is not limited thereto. The protection scope of the present application shall be based on the protection scope of the claims.
Claims
1. A biological sample freezing device, characterized in that: include: A liquid nitrogen tank, wherein the liquid nitrogen tank defines a containing cavity, and a top of the containing cavity has an inlet and an outlet; An outer shaft, the outer shaft extending in a vertical direction, the outer shaft defining an axis cavity, the outer shaft at least partially disposed in the accommodating cavity, the portion of the outer shaft located in the accommodating cavity being fixedly connected to a plurality of first freezing racks, the first freezing racks being used to store biological samples, the first freezing racks entering and exiting the accommodating cavity through the inlet and outlet; At least one bearing seat, the bearing seat is fixedly connected to the first end or the second end of the outer shaft, the bearing seat defines a bearing cavity, a bearing is disposed in the bearing cavity with upper and lower limits, and the outer ring of the bearing is fixedly connected to the bearing seat; an inner shaft, the inner shaft passing through the shaft cavity and at least partially located below the outer shaft, the inner shaft being fixedly connected to the inner ring of the bearing, the portion of the inner shaft below the outer shaft being fixedly connected to a plurality of second freezing racks, the second freezing racks also being used to store biological samples, the area corresponding to the first freezing rack being provided with a channel for the second freezing rack to pass through, the second freezing rack entering and exiting the accommodating cavity through the inlet and outlet and the channel; The first freezing rack and the second freezing rack both define a plurality of rack positions arranged vertically, the rack positions are used to place freezing boxes, the freezing boxes are used to place freezing tubes, and the freezing tubes are used to store biological samples; the top of the accommodating cavity has an axial hole, the first end of the outer shaft is arranged in the accommodating cavity, and the second end of the outer shaft passes through the axial hole and is arranged outside the accommodating cavity; The at least one bearing seat comprises a first bearing seat, the first bearing seat is fixedly connected to the first end of the outer shaft, the first bearing seat defines a first bearing cavity, a first bearing is disposed in the first bearing cavity, and an outer ring of the first bearing is fixedly connected to the first bearing seat; A first flange is fixed to the middle of the outer shaft, the first bearing seat is detachably fixedly connected to the first end of the outer shaft through threads, the lower end of the first flange and the upper end of the first bearing seat clamp and fix a first frame, and the first flange is fixedly connected to the first frame, and the first frame is used to place multiple first freezing racks.
2. The biological sample freezing device according to claim 1, characterized in that: The at least one bearing seat also includes a second bearing seat, which is fixedly connected to the second end of the outer shaft. The second bearing seat defines a second bearing cavity, in which a second bearing is arranged, and the outer ring of the second bearing is fixedly connected to the second bearing seat.
3. The biological sample freezing device according to claim 2, characterized in that: A first driving wheel is fixed to the upper end of the second bearing seat, and the first driving wheel is connected to a first driving mechanism to drive the outer shaft to rotate.
4. The biological sample freezing device according to claim 1, characterized in that: A third bearing is provided at the connection between the outer shaft and the shaft hole, the inner ring of the third bearing is fixed to the outer shaft, and the outer ring of the third bearing is fixed to the liquid nitrogen tank.
5. The biological sample freezing device according to claim 1, characterized in that: The first end of the inner shaft is located in the accommodating cavity, and the second end of the inner shaft passes through the shaft cavity and the shaft hole is located outside the accommodating cavity.
6. The biological sample freezing device according to claim 5, characterized in that: A second flange is fixed to the portion of the inner shaft below the outer shaft, a fixing piece is detachably fixedly connected to the first end of the inner shaft, a second frame is clamped and fixedly fixed by the lower end of the second flange and the upper end of the fixing piece, and the second flange is fixedly connected to the second frame, and the second frame is used to place a plurality of the second freezing racks.
7. The biological sample freezing device according to claim 5, characterized in that: A second driving wheel is fixed to the second end of the inner shaft, and the second driving wheel is connected to a second driving mechanism to drive the inner shaft to rotate.