A kind of application about selenolysine

Abstract

The present invention provides an application of selenolysine, and the selenomethionine is applied to resist plant viruses. Specifically, selenomethionine is applied to prepare a drug for preventing and treating plant viruses. The plant virus is tobacco mosaic virus (TMV). Selenomethionine is also applied to prepare a pharmaceutical composition. The pharmaceutical composition is applied to prevent and treat plant viruses; in the present invention, selenomethionine is used to effectively inhibit the proliferation of tobacco mosaic virus, and 0.5 mmol / L of selenomethionine inhibits the passivation activity of TMV by 72.15%, the protective activity by 65.39%, and the therapeutic activity by 45.26%. After tobacco is treated with selenomethionine, the growth state of tobacco is good, and there is no obvious inhibition or poor growth phenomenon, indicating that selenomethionine is safe for host plants and has no phytotoxic effect, indicating that selenomethionine has the potential to be developed into a plant virus inhibitor.

Description

Technical Field

[0001] The invention relates to the field of agricultural drugs, in particular to an application of selenolysine. Background Art

[0002] Plant virus disease, which is vividly called "plant cancer" because of its difficult-to-cure characteristics, is a disease caused by plant virus infection. The disease is widely found in key crops such as rice, vegetables, fruit trees and tobacco, posing a huge threat to agricultural production. Plant virus disease not only occurs frequently, but also spreads rapidly, with characteristics such as explosiveness, migration and intermittence. It is a global disease. In some cases, plant virus disease may even cause a 50% to 60% reduction in crop yields, or even a complete crop failure. At the same time, the continuous emergence of new plant viruses has also made prevention and control work more urgent and complicated.

[0003] At present, chemical control is one of the important means of controlling plant viruses. However, there is a relative shortage of control agents for plant virus diseases in the prior art, and the control effect on new viruses is limited. In addition, some control agents may affect the growth state of tobacco, and even cause inhibition or poor growth. Summary of the invention

[0004] In view of the shortcomings of the prior art, the purpose of the present invention is to provide an application of selenolysine to solve the problems raised in the above-mentioned background technology. After the tobacco is treated with selenomethionine, the growth state of the tobacco is good, and there is no obvious inhibition or poor growth phenomenon, indicating that selenomethionine is safe for host plants and has no phytotoxic effect.

[0005] In order to achieve the above object, the present invention is implemented through the following technical solutions: an application of selenolysine, applying selenomethionine to resist plant viruses.

[0006] Furthermore, selenomethionine is used in the preparation of drugs for preventing and treating plant viruses.

[0007] Furthermore, the plant virus is tobacco mosaic virus.

[0008] Furthermore, selenomethionine is used to prepare a pharmaceutical composition.

[0009] Furthermore, the pharmaceutical composition is used to prevent and treat plant viruses.

[0010] Beneficial effects of the present invention:

[0011] 1. The present invention evaluates the anti-plant virus activity of selenomethionine by the half-leaf spot method. The results show that selenomethionine effectively inhibits the proliferation of TMV. 0.5 mmol / L selenomethionine inhibits the passivation activity of TMV by 72.15%, the protective activity by 65.39%, and the therapeutic activity by 45.26%, and exhibits dose-dependency.

[0012] 2. After the present invention applies selenolysine to selenomethionine-treated tobacco, the tobacco grows well without obvious inhibition or poor growth, indicating that selenomethionine is safe for host plants and has no phytotoxic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is the molecular formula of the selenomethionine compound of the present invention;

[0014] Figure 2 This is a schematic diagram of the passivation activity of selenomethionine in the present invention in inhibiting TMV;

[0015] Figure 3 This is a schematic diagram of the protective activity of selenomethionine in the present invention in inhibiting TMV;

[0016] Figure 4 Schematic diagram of the therapeutic activity of selenomethionine in the present invention in inhibiting TMV. DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0018] See also Figures 1 to 4 The present invention provides the following technical solutions: an application of selenolysine, which applies selenomethionine to resist plant viruses.

[0019] The plant virus is tobacco mosaic virus.

[0020] The drug ingredient needed to fight plant viruses is selenomethionine.

[0021] The application test of selenomethionine in inhibiting plant viruses comprises the following steps:

[0022] 1) Dissolve selenomethionine compound in distilled water to concentrations of 0.1, 0.25 and 0.5 mmol / L;

[0023] 2) The selenomethionine compound solution was mixed with TMV extract at different preset concentrations, and then the mixture was applied to one side of the tobacco leaves. As a control, we only applied TMV extract without selenomethionine on the other side of the leaves. On the fourth day of the experiment, we observed and recorded the growth on both sides of the leaves, especially the number of lesions. By comparing the number of lesions, we can calculate the passivation activity of selenomethionine compounds on TMV, that is, its ability to inhibit viral replication or reduce leaf lesions caused by viruses;

[0024] 3) The selenomethionine compound solution was applied to one side of the healthy tobacco leaves at different concentrations, and the other side was not treated as a reference. On the second day, an equal amount of TMV extract was applied to both sides of the tobacco leaves, and the culture was continued for 4 days. The growth of both sides of the leaves was observed, and the protective activity of the selenomethionine compound in inhibiting TMV was calculated by the number of leaf lesions;

[0025] 4) Apply selenomethionine compound solution at different concentrations to one side of tobacco leaves infected with TMV, and leave the other side untreated as a reference. Observe the growth of both sides of the leaves on the 4th day, and calculate the therapeutic activity of selenomethionine compound in inhibiting TMV by the number of leaf lesions;

[0026] 5) Calculate the inhibition rate of selenomethionine compounds on TMV by the number of leaf lesions (the calculation formula is: inhibition rate (%) = [(CT) / C] × 100%, where "C" represents the average number of lesions in the control half leaf, and "T" represents the average number of lesions in the half leaf treated with selenomethionine);

[0027] 6) Obtain the results: evaluate the passivation activity of selenomethionine in inhibiting TMV, evaluate the protective activity of selenomethionine in inhibiting TMV, evaluate the therapeutic activity of selenomethionine in inhibiting TMV and evaluate the toxicity of selenomethionine to tobacco;

[0028] like Figure 2 As shown in the figure, the experimental steps for evaluating the inhibitory activity of selenomethionine on TMV inactivation are as follows:

[0029] Incubate the virus: Add TMV extract (TMV final concentration is 1.0 μg / mL) to the selenomethionine aqueous solution, mix gently, incubate on ice for 30 min, and add the same concentration of TMV extract to water as the control group;

[0030] Friction inoculation: Use Nicotiana tabacum at the 6-7 leaf stage as the test tobacco, add an appropriate amount of quartz sand to the leaves of the test Nicotiana tabacum, use a gauze swab to apply 100 μL of selenomethionine and virus mixture treatment group on the right half of the tobacco leaf along the main vein of the leaf, and apply 100 μL of control group on the left half of the leaf. Count the number of local leaf lesions on the 4th day after inoculation.

[0031] like Figure 3 As shown in the figure, the experimental steps for evaluating the protective activity of selenomethionine against TMV are as follows:

[0032] Drug application: Sansi tobacco at the 6-7 leaf stage was used as the test tobacco, 100 μL of 0.1, 0.25 and 0.5 mmol / L selenomethionine aqueous solution was taken and smeared on the right half of the tobacco leaf along the main vein of the leaf through a gauze swab, and then cultured at 25°C for 1 day, and distilled water was applied to the left half of the leaf as the blank control group;

[0033] Virus smearing: Add an appropriate amount of quartz sand to the test leaves of Nicotiana tabacum, take 100 μL of TMV extract (final TMV concentration is 1.0 μg / mL), and smear it on the surface of Nicotiana tabacum leaves with a gauze swab. Count the number of local leaf lesions on the fourth day after inoculation.

[0034] like Figure 4 As shown, the experimental steps for evaluating the therapeutic activity of selenomethionine in inhibiting TMV are as follows:

[0035] Apply virus: add an appropriate amount of quartz sand to the test Sansi tobacco leaves, take 100 μL TMV extract (TMV final concentration is 1.0 μg / mL), apply it on the right half of the tobacco leaf along the main vein of the leaf through a gauze swab, and continue to culture at 25°C for one day;

[0036] Apply the drug: Take 100 μL of selenomethionine compound and apply it on the surface of Nicotiana benthamiana leaves using a gauze swab. Continue to culture for 4 days and count the number of local leaf lesions.

[0037] The test results show that the anti-plant virus activity of selenomethionine was evaluated by the half-leaf spot method. The results showed that selenomethionine effectively inhibited the proliferation of tobacco mosaic virus TMV. The passivation inhibition activity of 0.5mmol / L selenomethionine on TMV reached 72.15%, the protective activity reached 65.39%, and the therapeutic activity reached 45.26%. After tobacco was treated with selenomethionine, the growth state of tobacco was good, with no obvious inhibition or poor growth, indicating that selenomethionine is safe for host plants and has no phytotoxic effect, indicating that selenomethionine has the potential to be developed into a plant virus inhibitor.

[0038] The basic principles and main features of the present invention and the advantages of the present invention are shown and described above. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-mentioned exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention.

[0039] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An application of selenolysine, characterized in that: Selenomethionine is used to fight plant viruses.

2. The use of selenolysine according to claim 1, characterized in that: Application of selenomethionine in the preparation of drugs for preventing and controlling plant viruses.

3. The use of selenolysine according to claim 1, characterized in that: The plant virus is tobacco mosaic virus.

4. The use of selenolysine according to claim 1, characterized in that: Selenomethionine is used in preparing pharmaceutical compositions.

5. The pharmaceutical composition according to claim 4, characterized in that: The pharmaceutical composition is used for preventing and controlling plant viruses.

Images