Method for preventing and treating ginger blast of tender ginger by brevibacillus laterosporus and application

Abstract

The invention relates to the technical field of microorganisms, in particular to a method for preventing and treating ginger blast of tender ginger through brevibacillus laterosporus and application of the method. The preservation number of the brevibacillus laterosporus H1 is CCTCC (China Center For Type Culture Collection) CNO (China Center For Type Culture Collection): M 20232065. The brevibacillus laterosporus H1 has the capability of preventing and treating the ginger blast of the tender ginger and the application method of the brevibacillus laterosporus H1 for the first time, a certain reference is provided for later research of the brevibacillus laterosporus H1, and the brevibacillus laterosporus H1 has important significance on microbial prevention and treatment of the ginger blast of the tender ginger.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a method and application of Brevibacillus laterosporus for preventing and controlling ginger blight of young ginger. Background Art

[0002] Ginger wilt is a soil-borne disease caused by Ralstonia solanacearum. Since its discovery, domestic and foreign scholars have conducted extensive research on the infection and colonization of Ralstonia solanacearum on plants, pathogenic mechanisms, pathogenic factors, detection techniques, pathogenesis, pathogenic factors and prevention and control techniques. The prevention and control methods of ginger wilt mainly include three categories: chemical control, agricultural control and biological control. Chemical control is the use of chemical agents to treat the cultivation soil. This method can effectively kill the pathogenic bacteria of ginger wilt, but it also causes devastating damage to the beneficial microorganisms in the soil and destroys the ecological balance of the soil. Biological control is a method of treating the cultivation soil with beneficial microorganisms or related products. This method can effectively control ginger wilt without causing damage to the soil. However, in the prior art, there are few reports on microbial strains for preventing and controlling ginger wilt in young ginger. Summary of the invention

[0003] The purpose of the present invention is to provide a method and application of Brevibacillus laterosporus for preventing and treating young ginger blight.

[0004] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0005] On the one hand, the invention provides the use of Brevibacillus laterosporus in preventing and treating young ginger blight. The deposit number of Brevibacillus laterosporus H1 is CCTCCNO: M20232065.

[0006] On the other hand, a method for preventing and controlling young ginger blight by Brevibacillus laterosporus is provided, comprising the following steps:

[0007] After the ginger was planted and covered with soil, the surface soil was irrigated with Brevibacillus laterosporus inoculant at a rate of 1×10 7 CFU / L, water consumption is 80L / 667m 2 -90L / 667m 2After spraying, wait for the soil surface to dry and then cover with film for heating;

[0008] 20-30 days before the young ginger is put on the market in the next year, the film is removed and the Brevibacillus laterosporus fungicide is sprayed once or twice at a concentration of 1×10 7 CFU / mL, the dosage is 2×10 12 CFU / 667m 2 -3×10 12 CFU / 667m 2 .

[0009] Furthermore, before ginger is planted, chlorine dioxide disinfectant is sprayed on the soil.

[0010] Furthermore, before digging trenches for ginger planting, chlorine dioxide disinfectant should be used for soil surface irrigation 10 days in advance, and the amount of irrigation should be enough to moisten the 5cm-8cm surface layer of the soil; after digging trenches and before planting, chlorine dioxide disinfectant should be sprayed on the surface soil in the trench again, with a water consumption of 30L / 667m 2 -35L / 667m 2 , sow at intervals of 5-8 days.

[0011] Furthermore, young ginger is cultivated through greenhouse planting and heating facilities, pipes are buried in the greenhouse, and water is circulated through a boiler to increase the temperature of the ginger.

[0012] The beneficial effects of the present invention are:

[0013] The present invention discloses for the first time that Brevibacillus laterosporus H1 has the ability to prevent and treat young ginger blight and an application method, which provides a certain reference for later research on Brevibacillus laterosporus and is of great significance for the microbial prevention and treatment of young ginger blight. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the colony morphology of Brevibacillus laterosporus;

[0015] Figure 2 It is the biolog identification comparison map of Brevibacillus laterosporus H1 strain;

[0016] Figure 3 This graph shows the antagonistic effect of Brevibacillus laterosporus H1 on the growth of Ralstonia solanacearum and Xanthomonas oryzae. DETAILED DESCRIPTION

[0017] The specific implementation modes of the present invention are described below so that those skilled in the art can understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific implementation modes. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the attached claims, these changes are obvious, and all inventions and creations utilizing the concept of the present invention are protected.

[0018] Example

[0019] 1. Strain source

[0020] The Brevibacillus laterosporus in this example was isolated from the composted soil after the test of inoculating various pathogens such as Brassica clubroot, rapeseed sclerotinia, and Rhizoctonia solani was completed. The specific isolation steps were as follows: taking a total of not less than 20 g of soil from 5 cm below the surface of the composted soil using a five-point sampling method with a sterile key, mixing it, weighing 10 g of it and dissolving it in 90 mL of pre-prepared sterile water, activating it at 28°C, 180 rpm, and dark conditions for 30 min, and gradiently diluting it to 10 8 , take 100 μL of the dilution and spread it evenly on the culture dish containing NA medium prepared in advance, culture at 30℃ for 24h, and observe the colony morphology ( Figure 1 ), pick a single colony strain for separation and purification to obtain a pure culture.

[0021] 2. Identification methods

[0022] After the strains were isolated and purified, they were compared by biolog Figure 2 ) was combined with 16S rDNA sequencing to identify the strain, and the similarity with the 16S rDNA sequence of Brevibacillus laterosporus was 99.05%. It was identified as Brevibacillus laterosporus and named H1. The strain was deposited in the China Center for Type Culture Collection (Address: Wuhan University, Wuhan, China) on October 30, 2023, with the collection number CCTCC NO: M 20232065.

[0023] 3. Indoor antagonistic effect on pathogens

[0024] According to laboratory tests, Brevibacillus laterosporus H1 has a significant antagonistic effect on the growth of multiple pathogens. The growth inhibition rates of Botrytis cinerea, Fusarium oxysporum and Leptosphaeria biglobosa were 39.26%, 54.46% and 70.51% respectively; the growth inhibition effect on Xanthomonas oryzae pv.oryzae and Ralstonia solanacearum was significant, and was significantly better than strains A1, TN4 and B22 ( Figure 3 ). The transparent zone (antagonistic zone) formed by inhibiting the growth of Ralstonia solanacearum, the pathogen of ginger blast, reached 8mm, and the growth inhibition effect was significant.

[0025] Test example

[0026] This test case is to plant young ginger in a continuous cropping mode and off-season. Specifically, young ginger is cultivated through greenhouse planting and heating facilities. Pipes are buried in the greenhouse, and water is circulated through the boiler to heat the ginger and maintain the ground temperature at around 25°C. This planting method allows ginger to be sown in October and harvested after New Year's Day. The wholesale price is more than 5 times higher than that of later ginger. However, the cultivation of young ginger in heating facilities is different from field cultivation. It is inconvenient to apply pesticides. During the heating period, the insulation film cannot be opened to apply pesticides. Pesticides can only be applied before planting and after the heating is stopped. If chemical control is applied, the use of drugs before planting is ineffective. If drugs are used 20 days before listing, the time is short and the effect cannot be shown. In addition, there is a hidden danger of residual drugs. In order to cooperate with the cultivation of young ginger in heating facilities, realize the prevention and control of young ginger blight and increase the yield of young ginger, the method for the prevention and control of young ginger blight in this test example is as follows:

[0027] Before digging trenches for ginger planting, 10 days in advance, use a 1000-fold dilution of chlorine dioxide disinfectant (available for livestock enclosures) to irrigate the soil surface. The amount of irrigation should be enough to moisten the top 5 cm of the soil. After digging trenches and before planting, spray the chlorine dioxide disinfectant on the surface soil in the trench again, with a water consumption of 30L / mu, and plant 5-8 days apart.

[0028] After ginger planting and soil covering, Brevibacillus laterosporus H1 was used to irrigate the surface soil to further inhibit the growth of ginger blight pathogens and regulate the soil microbial community structure. The application rate was 1×10 7 CFU / L, water consumption is 90L / 667m 2After spraying, wait for the soil surface to dry and then cover it with film for heating.

[0029] 20-30 days before the young ginger is put on the market in the next year, the film is removed and the Brevibacillus laterosporus H1 agent is sprayed once or twice at a concentration of 1×10 7 CFU / mL, the dosage is 2×10 12 CFU / 667m 2 .

[0030] At the same time, only chlorine dioxide disinfectant, only Brevibacillus laterosporus H1, conventional chemical control, and CK (no control) were set as control groups. The effects of preventing and controlling young ginger blight and increasing young ginger yield are shown in Table 1 below.

[0031] Table 1 Effects of different treatments on prevention and treatment of ginger blight and increase in ginger yield

[0032]

[0033] Note: The average original planting amount of ginger is about 20t / mu, i.e. 30kg / m 2 .

[0034] As can be seen from the above table, Brevibacillus laterosporus H1 has significant effects on preventing ginger blight and increasing the yield of early ginger. The present invention discloses for the first time that Brevibacillus laterosporus has the ability to prevent and treat ginger blight in young ginger, which provides a certain reference for the later research of Brevibacillus laterosporus and is of great significance for the microbial control of ginger blight in young ginger.

[0035] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. The application of Brevibacillus laterosporus in preventing and treating ginger blight of young ginger, characterized in that: The deposit number of Brevibacillus laterosporus H1 is CCTCC NO: M20232065.

2. A method for preventing and treating ginger blight of young ginger using Brevibacillus laterosporus, characterized in that: The following steps are involved: After the ginger was planted and covered with soil, the surface soil was irrigated with Brevibacillus laterosporus inoculant at a rate of 1×10 7 CFU / L, water consumption is 80L / 667m 2 -90L / 667m 2 After spraying, wait for the soil surface to dry and then cover with film for heating; 20-30 days before the young ginger is put on the market in the next year, the film is removed and the Brevibacillus laterosporus fungicide is sprayed once or twice at a concentration of 1×10 7 CFU / mL, the dosage is 2×10 12 CFU / 667m 2 -3×10 12 CFU / 667m 2 .

3. The method for preventing and treating young ginger blight by Brevibacillus laterosporus according to claim 2, characterized in that: Before planting ginger, spray chlorine dioxide disinfectant on the soil.

4. The method for preventing and treating young ginger blight by Brevibacillus laterosporus according to claim 3, characterized in that: Before digging trenches for ginger planting, chlorine dioxide disinfectant should be used for soil surface irrigation 10 days in advance. The amount of irrigation should be enough to moisten the 5cm-8cm surface of the soil. After digging trenches and before planting, spray the surface soil in the trench with chlorine dioxide disinfectant again, with a water consumption of 30L / 667m 2 -35L / 667m 2 , sow at intervals of 5-8 days.

5. The method for preventing and treating young ginger blight using Brevibacillus laterosporus according to claim 2, characterized in that: Young ginger is cultivated through greenhouse planting and heating facilities. Pipes are buried in the greenhouse and boilers are used to circulate water to increase the temperature of the ginger.

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