A prebiotic composition and its preparation and application in maintaining probiotic activity and promoting probiotic proliferation

Abstract

The present invention belongs to the technical field of functional / health food, and specifically relates to a prebiotic composition and its preparation and application in maintaining probiotic activity and promoting probiotic proliferation. The prebiotic composition includes 1-20 parts of sugar alcohol, 1-10 parts of sustained-release embedding material, and 20-100 parts of prebiotics by weight; the prebiotics are oligofructose, oligoxylose, oligomaltosan and stachyose; the mass ratio of oligofructose, oligoxylose, oligomaltosan and stachyose is 2:1:1:2. And the composition is granulated twice, has good solubility, can effectively promote the proliferation of probiotics and maintain their activity, and improves the effectiveness of probiotics.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional / health food, and specifically relates to a prebiotic composition and its preparation and application in maintaining the activity of probiotics and promoting the proliferation of probiotics. Background Art

[0002] Prebiotics refer to food ingredients that are generally not digested and absorbed by the human body but can be selectively utilized by human microorganisms, which can improve the composition and / or activity of intestinal microorganisms and thus benefit human health. That is, prebiotics need to be metabolized by microorganisms before they can be selectively utilized by human microorganisms to improve human health.

[0003] Probiotics are a type of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestines and reproductive system, can produce definite health effects, thereby improving the host's microecological balance and exerting beneficial effects on the intestines. It can be seen that probiotic products have the function of regulating the human intestine when there is a certain amount of "living state". However, due to the high sensitivity of probiotics to temperature, moisture, oxygen environment conditions, etc., powders in solid dosage forms are the mainstream choice of probiotic products on the market, among which powders that can be eaten directly are the most in line with the characteristics of probiotic foods.

[0004] In the formulation design of probiotic powders, there are many products on the market that combine prebiotics and probiotics. Adding prebiotics to probiotic formulas is intended to provide a suitable microenvironment for the growth and reproduction of probiotics. When prebiotics and probiotics are taken at the same time, they can be decomposed and utilized by probiotics as substrates, promoting the growth and reproduction of probiotics, thereby improving the effectiveness of probiotics.

[0005] However, most of the products on the market are simple combinations of probiotics and prebiotics, which are only simply mixed in the process. There is a certain degree of arbitrariness and blindness in the selection and combination of prebiotics. Not only do they fail to consider the solubility of prebiotics in the mouth when consumed directly, but also their storage stability. They also fail to consider whether the combination of prebiotics selected can effectively achieve the effective quantity and activity of the supplemented probiotics. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a prebiotic composition, which has good solubility and can effectively improve the proliferation time and proliferation amount of probiotics.

[0007] On the one hand, the present invention provides a prebiotic composition, which comprises, by weight, 1-20 parts of sugar alcohol, 1-10 parts of sustained-release embedding materials, and 20-100 parts of prebiotics; the prebiotics comprise oligofructose, oligoxylose, oligomaltose and stachyose; the mass ratio of the oligofructose, oligoxylose, oligomaltose and stachyose is 2:1:1:2; the prebiotic composition is prepared by a secondary granulation process.

[0008] Specifically, the prebiotic composition comprises, by weight, 5-15 parts of sugar alcohol, 3-8 parts of sustained-release embedding materials, and 50-90 parts of prebiotics.

[0009] Preferably, the prebiotic composition comprises, by weight, 5-10 parts of sugar alcohol, 3-5 parts of sustained-release embedding material, and 70-90 parts of prebiotics.

[0010] Specifically, the sugar alcohol is one or more of erythritol, lactitol, maltitol, sorbitol and isomalt;

[0011] The sustained-release embedding material is one or more of sodium alginate, sodium carboxymethyl cellulose, gelatin, peach gum and gum arabic.

[0012] Preferably, the sugar alcohol is erythritol; and the sustained-release embedding material is sodium alginate.

[0013] In another aspect, the present invention provides a method for preparing the aforementioned prebiotic composition, comprising the following steps:

[0014] S1, mixing stachyose, fructooligosaccharides, isomaltooligosaccharides and xylooligosaccharides to obtain a mixed material 1;

[0015] S2, dissolving sodium alginate to obtain a first layer of adhesive; dissolving erythritol to obtain a second layer of adhesive;

[0016] S3, preheating the above-mentioned mixed material 1;

[0017] S4, enriching and embedding the mixed material 1 in step S3 with the first layer of adhesive to obtain A;

[0018] S5, enriching and embedding the A in step S4 with the second layer of adhesive to obtain B;

[0019] S6, drying the above B to obtain C;

[0020] S7, sieving the above C to obtain a prebiotic composition.

[0021] Specifically, the preheating conditions in step S3 are: inlet air temperature 60-90°C, fan frequency 30Hz-45Hz, and material temperature 45-60°C.

[0022] Preferably, the inlet air temperature is 70-80°C and the fan frequency is 35Hz-45Hz.

[0023] Further preferably, the inlet air temperature is 70-75°C and the fan frequency is 38Hz-45Hz.

[0024] Specifically, the enrichment and embedding methods in steps S4 and S5 are carried out using an atomizing spray gun; the setting conditions of the atomizing spray gun are: atomizing pressure 0.1MPa-0.3MPa, flow rate 100mL / min-400mL / min.

[0025] Preferably, the setting conditions of the atomizing spray gun are: atomizing pressure 0.15MPa-0.2MPa, flow rate 250mL / min-350mL / min.

[0026] Specifically, the drying conditions in step S6 are: temperature 60° C.-75° C., to a moisture content of less than 2.2%; and the sieve aperture in step S7 is 1.0 mm-2.0 mm.

[0027] Preferably, the aperture of the sieve used in step S7 is 1.5 mm-2.0 mm.

[0028] Further preferably, the aperture of the sieve used in step S7 is 1.8 mm-2.0 mm.

[0029] In another aspect, the present invention provides the use of the aforementioned prebiotic composition or preparation method in maintaining the activity of probiotics and promoting the proliferation of probiotics.

[0030] Specifically, the probiotics include but are not limited to: one or more of Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum or Lactobacillus acidophilus.

[0031] In yet another aspect, the present invention provides a probiotic powder comprising the aforementioned prebiotic composition and probiotics.

[0032] Specifically, the probiotics include but are not limited to: one or more of Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum or Lactobacillus acidophilus.

[0033] The technical effects achieved by the present invention are:

[0034] (1) The prebiotic composition provided by the present invention can effectively enhance the proliferation effect of probiotics.

[0035] (2) The prebiotic composition provided by the present invention prolongs the proliferation time of probiotics in the intestine.

[0036] (3) The prebiotic composition provided by the present invention has excellent rapid solubility.

[0037] (4) The prebiotic composition provided by the present invention presents a multi-dimensional porous structure, which can protect the probiotics and improve the stability of the probiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 The proliferation effects of Example 1 and Comparative Examples 1-10 on Bifidobacterium lactis.

[0039] Figure 2 The figure shows the proliferation effect of Example 1 and Comparative Examples 1-10 on Lactobacillus paracasei.

[0040] Figure 3 The proliferation effects of Example 1 and Comparative Examples 1-10 on Lactobacillus plantarum are shown.

[0041] Figure 4 The proliferation effects of Example 1 and Comparative Examples 1-10 on Lactobacillus acidophilus are shown. DETAILED DESCRIPTION

[0042] The present invention will be further described in detail below in conjunction with specific examples. The following examples are not intended to limit the present invention, but are only intended to illustrate the present invention. The experimental methods used in the following examples are generally conventional, unless otherwise specified, and the materials, reagents, etc. used in the following examples are commercially available, unless otherwise specified.

[0043] The source of the strain used in the present invention is:

[0044] Bifidobacterium lactis (HN019), purchased from Danisco (China) Co., Ltd.;

[0045] Lactobacillus paracasei (Lpc-37), purchased from Danisco (China) Co., Ltd.;

[0046] Lactobacillus plantarum (Lp-115), purchased from Danisco (China) Co., Ltd.;

[0047] Lactobacillus acidophilus (NCFM) was purchased from Danisco (China) Co., Ltd.

[0048] Example 1

[0049] The sample of this example is a prebiotic composition 1 obtained by granulating 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of oligoisomaltose, and 15 parts by weight of oligoxylose with 5 parts by weight of sodium alginate and 5 parts by weight of erythritol twice. The specific preparation steps are as follows:

[0050] (1) 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of isomaltooligosaccharide, and 15 parts by weight of oligoxylose are mixed and put into a material pot of a fluidized bed granulation equipment to form a mixed material 1;

[0051] (2) taking 5 parts by weight of sodium alginate and using purified water to prepare a solution with a concentration of 1%wt as the first layer of adhesive;

[0052] (3) Turn on the fluidized bed granulation equipment, preheat the mixed material 1 in the material pot of the fluidized bed equipment, set the inlet air temperature to 60-90° C. (75° C. in this embodiment), the fan frequency to 30 Hz-45 Hz (42 Hz in this embodiment), and the material temperature to 45-60° C. (45-60° C. in this embodiment);

[0053] (4) After the temperature of the mixed material 1 in the material pot of the fluidized bed equipment reaches the set temperature range, the sodium alginate solution is sprayed into the fluidized bed through an atomizing spray gun to enrich and embed the mixed material 1, with an atomizing pressure of 0.1 MPa-0.3 MPa (0.2 MPa in this embodiment) and a flow rate of 100 mL / min-400 mL / min (260 mL / min in this embodiment);

[0054] (5) taking 5 parts by weight of erythritol and using purified water to prepare a solution with a concentration of 10%wt as the second layer of adhesive;

[0055] (6) After the first layer of adhesive is sprayed, adjust the atomization pressure to 0.15 MPa and the flow rate to 320 mL / min, and continue to spray the erythritol solution;

[0056] (7) After spraying the second layer of adhesive, the mixed material 1 in the material pot is dried at a drying temperature of 60° C.-75° C. (60-75° C. in this embodiment) until the moisture content is less than 2.2% (less than 2.2% in this embodiment);

[0057] (8) After drying, the dried mixed material 1 in the material pot is sieved and granulated, and the sieve has a pore size of 1.0 mm-2.0 mm (2.0 mm in this embodiment);

[0058] (9) After whole granules, the prebiotic composition 1 is obtained.

[0059] Comparative Example 1

[0060] The sample of this comparative example is a prebiotic composition 2 prepared by mixing and drying 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of isomaltooligosaccharide, 15 parts by weight of oligoxylose, 5 parts by weight of sodium alginate, and 5 parts by weight of erythritol. The specific steps are as follows:

[0061] (1) 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of isomaltooligosaccharide, 15 parts by weight of xylo-oligosaccharide, 5 parts by weight of sodium alginate, and 5 parts by weight of erythritol are mixed and put into a material pot of a fluidized bed granulation equipment, which is referred to as mixed material 2;

[0062] (2) Turn on the fluidized bed granulation equipment, heat and dry the mixed material 2 in the material pot of the fluidized bed equipment, set the air inlet temperature to 60-90° C., the fan frequency to 30 Hz-45 Hz, and the drying temperature to 60° C.-75° C., until the moisture content is below 2.2%;

[0063] (3) After drying, a prebiotic composition 2 is obtained.

[0064] Comparative Example 2

[0065] The sample of Comparative Example 2 is a single prebiotic prepared with 100 parts by weight of stachyose.

[0066] Comparative Example 3

[0067] The sample of this comparative example 3 is a single prebiotic prepared with 100 parts by weight of oligofructose.

[0068] Comparative Example 4

[0069] The sample of this comparative example 4 is a single prebiotic prepared with 100 parts by weight of isomaltooligosaccharide.

[0070] Comparative Example 5

[0071] The sample of Comparative Example 5 is a single prebiotic prepared with 100 parts by weight of xylo-oligosaccharide.

[0072] Comparative Example 6

[0073] The sample of Comparative Example 6 is a single prebiotic prepared with 100 parts by weight of polydextrose.

[0074] Comparative Example 7

[0075] The sample of Comparative Example 7 is a single prebiotic prepared with 100 parts by weight of resistant dextrin.

[0076] The single prebiotics in Comparative Examples 2-7 are all in powder form and do not need to be processed.

[0077] Comparative Example 8

[0078] The difference between Comparative Example 8 and Example 1 is that the prebiotic composition does not contain stachyose (that is, stachyose is replaced by isomaltooligosaccharide of equal weight), and the rest is the same as Example 1. The details are as follows:

[0079] The sample of this comparative example is a prebiotic composition of 30 parts by weight of oligofructose, 45 parts by weight of oligomaltosaccharide, and 15 parts by weight of oligoxylose, and 5 parts by weight of sodium alginate and 5 parts by weight of erythritol are granulated twice to obtain a prebiotic composition 3. The specific preparation steps are as follows:

[0080] (1) 30 parts by weight of oligofructose, 45 parts by weight of oligomalto-oligosaccharide, and 15 parts by weight of oligoxylose are mixed and put into a material pot of a fluidized bed granulation equipment to form a mixed material 3;

[0081] (2) taking 5 parts by weight of sodium alginate and using purified water to prepare a solution with a concentration of 1%wt as the first layer of adhesive;

[0082] (3) Turn on the fluidized bed granulation equipment, preheat the mixed material 3 in the material pot of the fluidized bed equipment, set the inlet air temperature to 75° C., the fan frequency to 42 Hz, and the material temperature to 45-60° C.;

[0083] (4) After the temperature of the mixed material 3 in the material pot of the fluidized bed equipment reaches the set temperature range, the sodium alginate solution is sprayed into the fluidized bed through an atomizing spray gun to enrich and embed the mixed material 3. The atomizing pressure is 0.2 MPa and the flow rate is 260 mL / min;

[0084] (5) taking 5 parts by weight of erythritol and using purified water to prepare a solution with a concentration of 10%wt as the second layer of adhesive;

[0085] (6) After the first layer of adhesive is sprayed, adjust the atomization pressure to 0.15 MPa and the flow rate to 320 mL / min, and continue to spray the erythritol solution;

[0086] (7) After spraying the second layer of adhesive, the mixed material 3 in the material pot is dried at a drying temperature of 60° C. to 75° C. until the moisture content is below 2.2%;

[0087] (8) After drying, the dried mixed material 3 in the material pot is sieved and granulated, and the sieve is selected with a pore size of 2.0 mm;

[0088] (9) After whole granulation, the prebiotic composition 3 is obtained.

[0089] Comparative Example 9

[0090] The difference between Comparative Example 9 and Example 1 is that the prebiotic composition does not contain isomaltooligosaccharide (i.e., isomaltooligosaccharide is replaced with stachyose in equal weight), and the rest is the same as Example 1. The details are as follows:

[0091] The sample of this comparative example is a prebiotic composition of 30 parts by weight of oligofructose, 45 parts by weight of stachyose, and 15 parts by weight of oligoxylose, and 5 parts by weight of sodium alginate and 5 parts by weight of erythritol are granulated twice to obtain a prebiotic composition 4. The specific preparation steps are as follows:

[0092] (1) 30 parts by weight of oligofructose, 45 parts by weight of stachyose, and 15 parts by weight of oligoxylose are mixed and put into a material pot of a fluidized bed granulation equipment to form a mixed material 4;

[0093] (2) taking 5 parts by weight of sodium alginate and using purified water to prepare a solution with a concentration of 1%wt as the first layer of adhesive;

[0094] (3) Turn on the fluidized bed granulation equipment, preheat the mixed material 4 in the material pot of the fluidized bed equipment, set the inlet air temperature to 75° C., the fan frequency to 42 Hz, and the material temperature to 45-60° C.;

[0095] (4) After the temperature of the mixed material 4 in the material pot of the fluidized bed equipment reaches the set temperature range, the sodium alginate solution is sprayed into the fluidized bed through an atomizing spray gun to enrich and embed the mixed material 4. The atomizing pressure is 0.2 MPa and the flow rate is 260 mL / min;

[0096] (5) taking 5 parts by weight of erythritol and using purified water to prepare a solution with a concentration of 10%wt as the second layer of adhesive;

[0097] (6) After the first layer of adhesive is sprayed, adjust the atomization pressure to 0.15 MPa and the flow rate to 320 mL / min, and continue to spray the erythritol solution;

[0098] (7) After spraying the second layer of adhesive, the mixed material 4 in the material pot is dried at a drying temperature of 60° C. to 75° C. until the moisture content is below 2.2%;

[0099] (8) After drying, the dried mixed material 4 in the material pot is sieved and granulated, and the sieve is selected with a pore size of 2.0 mm;

[0100] (9) After whole granulation, the prebiotic composition 4 is obtained.

[0101] Comparative Example 10

[0102] The difference between Comparative Example 9 and Example 1 is that the prebiotic composition does not contain stachyose and isomaltooligosaccharide (that is, stachyose is replaced by an equal weight of xylo-oligosaccharide, and isomaltooligosaccharide is replaced by an equal weight of fructooligosaccharide), and the rest is the same as Example 1. The details are as follows:

[0103] The sample of this comparative example is a prebiotic composition 5 obtained by granulating 45 parts by weight of oligofructose and 45 parts by weight of oligoxylose as a prebiotic composition, and 5 parts by weight of sodium alginate and 5 parts by weight of erythritol twice. The specific preparation steps are as follows:

[0104] (1) 45 parts by weight of oligofructose and 45 parts by weight of oligoxylose are mixed and put into a material pot of a fluidized bed granulation equipment to form a mixed material 5;

[0105] (2) taking 5 parts by weight of sodium alginate and using purified water to prepare a solution with a concentration of 1%wt as the first layer of adhesive;

[0106] (3) Turn on the fluidized bed granulation equipment, preheat the mixed material 5 in the material pot of the fluidized bed equipment, set the inlet air temperature to 75° C., the fan frequency to 42 Hz, and the material temperature to 45-60° C.;

[0107] (4) After the temperature of the mixed material 5 in the material pot of the fluidized bed equipment reaches the set temperature range, the sodium alginate solution is sprayed into the fluidized bed through an atomizing spray gun to enrich and embed the mixed material 5. The atomizing pressure is 0.2 MPa and the flow rate is 260 mL / min;

[0108] (5) taking 5 parts by weight of erythritol and using purified water to prepare a solution with a concentration of 10%wt as the second layer of adhesive;

[0109] (6) After the first layer of adhesive is sprayed, adjust the atomization pressure to 0.15 MPa and the flow rate to 320 mL / min, and continue to spray the erythritol solution;

[0110] (7) After spraying the second layer of adhesive, the mixed material 5 in the material pot is dried at a drying temperature of 60° C. to 75° C. until the moisture content is below 2.2%;

[0111] (8) After drying, the dried mixed material 5 in the material pot is sieved and granulated, and the sieve is selected with a pore size of 2.0 mm;

[0112] (9) After whole granulation, the prebiotic composition 5 is obtained.

[0113] Effect Example 1

[0114] The solubility and taste evaluation of the prebiotics or prebiotic compositions of Example 1 and Comparative Examples 1-10 prepared above in the oral cavity were tested. The test method is as follows: 2 g of Example 1 and Comparative Examples 1-7 were taken respectively, and the samples were directly placed on the tongue surface, and a stopwatch was started to time the sample. The sample was rapidly dissolved in saliva, and swallowing was used to drive the sample to completely dissolve. The timer was stopped, and the dissolution time was recorded. At the same time, the taste evaluation of the direct taking process was recorded.

[0115] The results are shown in Table 1:

[0116] Table 1

[0117]

[0118]

[0119] Compared with Comparative Examples 2-7, Comparative Example 1 without embedding granulation, and Comparative Examples 8-10 with embedding granulation, the oral solubility and mouth feel of Example 1 are greatly improved.

[0120] Application Example 1

[0121] 1.1 Application of the embedded granulated prebiotic composition prepared in Example 1 in probiotic formulation

[0122] The embedded granulated prebiotic composition prepared in Example 1 was applied to the probiotic formula, that is, 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of isomaltooligosaccharide, 15 parts by weight of xylo-oligosaccharide, 5 parts by weight of sodium alginate, and 5 parts by weight of erythritol were granulated twice to obtain the prebiotic composition 1. 90 parts of the prebiotic composition 1 were taken, and 10 parts of 100 billion CFU / g Bifidobacterium lactis were added to prepare the 10 billion CFU / g probiotic powder A.

[0123] 1.2 Application of the unencapsulated granulated prebiotic composition prepared in Comparative Example 1 in probiotic formulation

[0124] The un-embedded granulated prebiotic composition prepared in Comparative Example 1 was applied to the probiotic formula, that is, 30 parts by weight of stachyose, 30 parts by weight of oligofructose, 15 parts by weight of isomaltooligosaccharide, and 15 parts by weight of xylo-oligosaccharide were used as the prebiotic composition, 5 parts by weight of sodium alginate and 5 parts by weight of erythritol were mixed and dried to obtain a prebiotic composition 2, 90 parts of the prebiotic composition 2 were taken, and 10 parts of 100 billion CFU / g Bifidobacterium lactis were added to prepare a probiotic powder B with a specification of 10 billion CFU / g.

[0125] 1.3 Results

[0126] Probiotic powder A and probiotic powder B were subjected to accelerated stability test at 37°C and 75% humidity for 3 months to detect the survival rate of Bifidobacterium lactis in the samples. The detection method was carried out according to the national standard GB4789.35.

[0127] The test results are shown in Table 2:

[0128] Table 2

[0129]

[0130]

[0131] The encapsulated granulated prebiotic composition was applied to the probiotic formula, and the survival rate was higher than that of the non-encapsulated granulated prebiotic combination after accelerated stability test at 37°C.

[0132] Application Example 2

[0133] 1.1 Proliferation duration and effect of prebiotic composition 1 of Example 1 on probiotics

[0134] The proliferative duration and effect of the prebiotic composition 1 of Example 1 on Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus acidophilus were tested as follows:

[0135] (1) Prepare MRS broth culture medium, 20%wt L-cysteine ​​hydrochloride solution, and 10%wt concentration prebiotic composition 1 solution.

[0136] (2) Take 0.1 g of Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus acidophilus, respectively, add them to 9 mL of buffer solution, mix well, and use a sterile inoculation loop to mark four areas on an MRS plate, and culture at 37°C under anaerobic conditions for 48 hours.

[0137] (3) Single colonies were picked from the cultured plates and cultured in 10 mL of MRS broth for 16 hours to obtain four culture solutions of Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus acidophilus.

[0138] (4) Use an ELISA reader to detect the OD600 concentration of the four bacterial cultures after culture.

[0139] (5) Add the prebiotic composition 1 solution into the glucose-free MRS broth medium at a ratio of 1% by weight.

[0140] (6) MRS broth without glucose and culture medium supplemented with prebiotics but without bacterial solution were used as blank controls.

[0141] (7) The four culture solutions of step (3) were taken at a ratio of 2% wt, and added to the glucose-free MRS broth medium of step (5), and 0.05% wt of L-cysteine ​​hydrochloride was additionally added to the glucose-free MRS broth medium added with the culture solution of Bifidobacterium lactis, and the mixture was mixed evenly.

[0142] (8) The glucose-free MRS broth medium without prebiotics was used as the negative control (containing bacterial solution).

[0143] (9) Add the culture solution obtained in steps (6), (7), and (8) to a 96-well plate and culture for 48 hours. Detect the OD600 concentration using an ELISA reader every 2 hours, and repeat three times in parallel.

[0144] (10) The OD600 value obtained after 48 hours of culture was used to calculate the OD600 value of the strain grown in different prebiotics according to the following formula.

[0145] (11) Strain growth OD600 = W2-W0-W1.

[0146] (12) Where: W1 is the OD600 value of the negative control group, W0 is the OD600 value of the blank control group, and W2 is the OD600 value after 48 hours of culture.

[0147] 1.2 Proliferation duration and effect of the prebiotics or prebiotic compositions of comparative examples 1-10 on probiotics

[0148] The prebiotics or prebiotic compositions of Comparative Examples 1-10 were tested for their proliferation duration and effects on Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus acidophilus, using the same method as in 1.1.

[0149] 1.3 Results

[0150] Test results see Figure 1-Figure 4 Compared with Comparative Examples 2-7, Comparative Example 1 without embedding granulation, and Comparative Examples 8-10 with embedding granulation, Example 1 has better proliferation time efficiency and proliferation enhancement effect on Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum, and Lactobacillus acidophilus.

Claims

1. A prebiotic composition, characterized in that The invention comprises 1-20 parts of sugar alcohol, 1-10 parts of sustained-release embedding materials and 20-100 parts of prebiotics by weight; the prebiotics comprise oligofructose, oligoxylose, oligomaltose and stachyose; the mass ratio of the oligofructose, oligoxylose, oligomaltose and stachyose is 2:1:1:2; and the prebiotic composition is prepared by a secondary granulation process.

2. The prebiotic composition according to claim 1, characterized in that The sugar alcohol is one or more of erythritol, lactitol, maltitol, sorbitol and isomalt; the sustained-release embedding material is one or more of sodium alginate, sodium carboxymethyl cellulose, gelatin, peach gum and gum arabic.

3. The prebiotic composition according to claim 1 or 2, characterized in that The sugar alcohol is erythritol; and the sustained-release embedding material is sodium alginate.

4. The method for preparing the prebiotic composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1, mixing stachyose, fructooligosaccharides, isomaltooligosaccharides and xylooligosaccharides to obtain a mixed material 1; S2, dissolving sodium alginate to obtain a first layer of adhesive; dissolving erythritol to obtain a second layer of adhesive; S3, preheating the above-mentioned mixed material 1; S4, enriching and embedding the mixed material 1 in step S3 with the first layer of adhesive to obtain A; S5, enriching and embedding the A in step S4 with the second layer of adhesive to obtain B; S6. Dry the above B to obtain C; S7, sieving the above C to obtain a prebiotic composition.

5. The preparation method according to claim 4, characterized in that: The preheating conditions in step S3 are: air inlet temperature 60-90°C, fan frequency 30Hz-45Hz, and material temperature 45-60°C.

6. The preparation method according to claim 4, characterized in that: The enrichment and embedding methods in steps S4 and S5 are carried out using an atomizing spray gun; the setting conditions of the atomizing spray gun are: atomizing pressure 0.1MPa-0.3MPa, flow rate 100mL / min-400mL / min.

7. The preparation method according to claim 4, characterized in that: The drying conditions in step S6 are: temperature 60°C-75°C, to a moisture content of less than 2.2%; the sieve aperture in step S7 is 1.0mm-2.0mm.

8. Use of the prebiotic composition according to any one of claims 1 to 3 or the preparation method according to any one of claims 4 to 7 in maintaining the activity of probiotics and promoting the proliferation of probiotics.

9. The use according to claim 8, characterized in that: The probiotics are one or more of Bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus plantarum or Lactobacillus acidophilus.

10. A probiotic powder, characterized in that: The invention comprises the prebiotic composition according to any one of claims 1 to 3 and probiotics.

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