Preparation method for improving quality of paper-making reconstituted tobacco and application

Abstract

The invention provides a preparation method for improving the quality of paper-making reconstituted tobacco, which comprises the following steps: A) carrying out steam explosion on a tobacco stem raw material to obtain a steam-exploded material; b) pretreating the material subjected to steam explosion by adopting a compound enzyme solution to obtain a material subjected to enzymolysis; the compound enzyme comprises one or more of laccase, pectinase and protease; and C) carrying out flash extraction on the material after enzymolysis, then filtering, concentrating, coating to a sheet base, drying and slitting to obtain a reconstituted tobacco product. The tobacco stems are treated through steam explosion and biological compound enzyme, the internal structure and chemical components of the tobacco stems are improved, the mixed materials are extracted in a flash mode, extraction can be completed within several minutes, and the efficiency is high. The reconstituted tobacco product provided by the invention is less in offensive odor, rich in fragrance and sufficient in cigarette smell. Not only is the sense good, but also the content of thermosensitive substances such as benzyl alcohol, damascenone, megastigmatrienone 2, furfural, solanone, neophytadiene, dihydroactinidiolide, ethyl palmitate and the like is high.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco, and in particular to a preparation method and application for improving the quality of papermaking reconstituted tobacco. Background Art

[0002] Papermaking reconstituted tobacco is mainly made of tobacco waste materials such as tobacco dust, tobacco stems, and tobacco fragments in the process of cigarette processing, which are recombined and processed. The production method of reconstituted tobacco is to soak tobacco stems, tobacco dust, and tobacco fragments in water, extract and separate the soluble and insoluble substances, and then grind the insoluble tobacco fibers or some wood pulp fibers called external fibers together, and then make them into substrates; the soluble extract is concentrated, added and modulated, applied and restored to the substrate, and dried and cut to make reconstituted tobacco.

[0003] Papermaking reconstituted tobacco has become an important raw material component of Chinese cigarettes due to its advantages such as tar reduction, harm reduction, adjustable and controllable, and low cost. However, in terms of the internal quality of reconstituted tobacco, domestic reconstituted tobacco has shortcomings such as insufficient flavor and aroma, heavy woody smell, strong irritation, uncoordinated smoke and poor softness. Therefore, it is of great significance to improve the sensory quality of papermaking reconstituted tobacco.

[0004] Raw material extraction is an important process step to ensure product yield, quality and cost. The extraction rate directly determines the amount of tobacco substances that are subsequently restored to the substrate, which in turn affects the quality of the reconstituted tobacco. Tobacco stems are one of the main raw materials for the papermaking process of reconstituted tobacco leaves. They can be used to extract tobacco extracts and provide fiber materials for making substrates. Tobacco stems contain high levels of macromolecular substances such as pectin and lignin, which not only affect the extraction of tobacco stem fibers, but are also closely related to the physical structure of the substrate, causing changes in the air permeability of the substrate, affecting the combustion performance of reconstituted tobacco leaves, and increasing the impurities in the reconstituted tobacco leaves, affecting the sensory quality.

[0005] When domestic enterprises use the papermaking method to reconstruct tobacco leaves, they mostly extract and pulp the tobacco stems. The extraction and pulping effects of tobacco stems are important factors affecting production efficiency and product quality. Currently, the most widely used extraction method is the three-stage countercurrent extraction technology, which facilitates the dissolution of soluble matter in tobacco stems and the subsequent pulping process.

[0006] Patent CN202011396603 discloses a method for extracting raw materials of reconstituted tobacco leaves by papermaking method, which uses a high-concentration pulper and an extraction screw machine to complete the extraction operation of soluble components in the raw materials. Since the extraction can be carried out at a high material concentration, compared with the extraction tank extraction of the extraction tank type, it can reduce the water consumption of the extraction process and the water consumption cost, and also reduce the floor space of the equipment and the difficulty of equipment maintenance. However, this method still has the problems of low extraction rate and long extraction time, and cannot improve product quality and production efficiency.

[0007] Therefore, there is an urgent need for a method for preparing papermaking reconstituted tobacco to improve product quality and further increase the production efficiency of reconstituted tobacco. Summary of the invention

[0008] In view of this, the technical problem to be solved by the present invention is to provide a preparation method for improving the quality of reconstituted tobacco leaves by a papermaking process. The reconstituted tobacco leaves provided by the present invention have good sensory properties and are high in the content of heat-sensitive substances such as benzyl alcohol, damascena, megastigmatrienone 2, furfural, solanone, neophytadiene, dihydroactin, and ethyl hexadecanoate.

[0009] The present invention provides a preparation method for improving the quality of papermaking process reconstituted tobacco, comprising the following steps:

[0010] A) steam-exploding a tobacco stem raw material to obtain a steam-exploded material;

[0011] B) pretreating the steam-exploded material with a composite enzyme solution to obtain an enzymatically hydrolyzed material; the composite enzyme comprises one or more of laccase, pectinase and protease;

[0012] C) The enzymatically hydrolyzed material is subjected to flash extraction, and then filtered, concentrated, coated onto a sheet base, dried, and cut to obtain a reconstituted tobacco leaf product.

[0013] Preferably, the parameters of the steam explosion in step A) include: saturated steam pressure of 0.8-1.2 MPa, and pressure maintenance time of 30-90 s.

[0014] Preferably, step B) specifically comprises: spraying the composite enzyme solution onto the steam-exploded material, and during the spraying process, rotating the feeding cylinder in the steam explosion device to mix the enzyme solution evenly;

[0015] The complex enzyme solution is an aqueous solution of a complex enzyme preparation, and the pH value is adjusted to 4-7 by using 1 mol / L citric acid solution; the mass concentration of the complex enzyme solution is 3wt%-6wt%.

[0016] Preferably, the mass ratio of laccase, pectinase and protease in step B) is (1-3):(0-1):(0-1);

[0017] The laccase activity is 10,000 to 20,000 U / g; the pectinase activity is 10,000 to 15,000 U / g; and the protease activity is 10,000 to 15,000 U / g.

[0018] Preferably, in step B), the enzymolysis temperature is 35-60° C. and the time is 1-3 h; after the enzymolysis is completed, the temperature is raised to 90° C. and the enzyme is inactivated for 10 min.

[0019] Preferably, the added amount of the complex enzyme in step B) is 5wt% to 8wt% of the steam-exploded material.

[0020] Preferably, the parameters of the flash extraction in step C) are: extraction time 1 to 3 min, material-liquid mass ratio 1:(4 to 6), extraction voltage 120 to 160 V.

[0021] Preferably, the filtration in step C) is gauze filtration; the concentration parameters include: concentration temperature of 50-60°C, concentration to liquid solid content of 38-42%; the drying parameters include drying temperature of 100-110°C, drying to material moisture of 10-12%.

[0022] The present invention provides a papermaking method reconstituted tobacco leaf, which is prepared by the preparation method described in any one of the above technical solutions.

[0023] The present invention provides a tobacco product, comprising the reconstituted tobacco leaves described in the above technical solution.

[0024] Compared with the prior art, the present invention provides a preparation method for improving the quality of papermaking reconstituted tobacco, comprising the following steps: A) steam-exploding the tobacco stem raw material to obtain a steam-exploded material; B) pre-treating the steam-exploded material with a composite enzyme solution to obtain an enzymatically hydrolyzed material; the composite enzyme includes one or more of laccase, pectinase and protease; C) flash-extracting the enzymatically hydrolyzed material, then filtering, concentrating, coating on a sheet base, drying, and cutting to obtain a reconstituted tobacco product. The present invention treats tobacco stems by steam explosion and biological composite enzymes to improve the internal structure and chemical composition of tobacco stems, and extracts the mixed material by flash extraction, which can be completed in a few minutes with high efficiency. The reconstituted tobacco product provided by the present invention has less impurities, rich aroma and sufficient smoke flavor. Not only is the sensory organoleptic property good, but also the content of heat-sensitive substances such as benzyl alcohol, damascena, megastigmatrienone 2, furfural, solanone, neophytadiene, dihydroactinolactone, hexadecanoic acid ethyl ester is high. DETAILED DESCRIPTION

[0025] The present invention provides a preparation method and application for improving the quality of reconstituted tobacco leaves by papermaking. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they all fall within the scope of protection of the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the method and application of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0026] It should be understood that the expression "one or more of..." includes each of the items recited after the expression individually and in various different combinations of two or more of the recited items, unless otherwise understood from the context and usage. The expression "and / or" in combination with three or more recited items should be understood to have the same meaning, unless otherwise understood from the context.

[0027] The use of the terms "comprising", "having" or "containing", including their grammatical synonyms, should generally be understood as open and non-restrictive, for example not excluding other unrecited elements or steps, unless otherwise specifically stated or otherwise understood from the context.

[0028] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0029] In this application, "at least one" means one or more, "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0030] The method firstly performs steam explosion on tobacco stems to effectively destroy the cell wall structure of tobacco stems, make the tissue structure of tobacco stems loose, and promote the degradation and transformation of exposed cellulose, hemicellulose and other substances; then uses biological composite enzyme to pretreat the exploded tobacco stems to reduce the content of macromolecular substances such as protein, pectin and lignin in the tobacco stems; then flash extracts tobacco stems, tobacco scraps and added fiber mixture to efficiently extract soluble substances, separate solid and liquid, beat the residue and make sheets, refine and concentrate the liquid, add flavors and then apply them to the sheet base, dry and cut them to obtain reconstituted tobacco leaf products.

[0031] The present invention provides a preparation method for improving the quality of papermaking process reconstituted tobacco, comprising the following steps:

[0032] A) steam-exploding a tobacco stem raw material to obtain a steam-exploded material;

[0033] B) pretreating the steam-exploded material with a composite enzyme solution to obtain an enzymatically hydrolyzed material; the composite enzyme comprises one or more of laccase, pectinase and protease;

[0034] C) The enzymatically hydrolyzed material is subjected to flash extraction, and then filtered, concentrated, coated onto a sheet base, dried, and cut to obtain a reconstituted tobacco leaf product.

[0035] The preparation method for improving the quality of papermaking reconstituted tobacco provided by the present invention firstly performs steam explosion on tobacco stem raw materials to obtain steam-exploded materials.

[0036] The present invention firstly performs steam explosion on tobacco stems, effectively destroying the cell wall structure of tobacco stems, making the tissue structure of tobacco stems loose, and promoting the degradation and conversion of exposed cellulose, hemicellulose and other substances.

[0037] The steam explosion of the present invention is preferably carried out in a steam explosion device; preferably, the tobacco stems are loaded into a vertical high-pressure sample introduction cylinder of the steam explosion device, and saturated water vapor is introduced to fully wet the raw materials, and then the discharge valve is quickly opened to discharge the materials into the storage tank, and then the materials are transferred to the feeding drum.

[0038] According to the present invention, the parameters of the steam explosion include: saturated steam pressure of 0.8-1.2 MPa, specifically 0.8 MPa, 0.9 MPa, 1.0 MPa, 1.1 MPa, 1.2 MPa; or a range value between any two of the above.

[0039] According to the present invention, the pressing time is 30 to 90 seconds; the pressing time is 30 seconds, 40 seconds, 50 seconds, 60 seconds, 70 seconds, 80 seconds or 90 seconds; or a range value between any two of the above.

[0040] The composite enzyme solution is used to pretreat the steam-exploded material to obtain the enzymolyzed material.

[0041] Specifically, the method comprises: spraying the composite enzyme solution onto the steam-exploded material, and rotating the feeding cylinder in the steam explosion device during the spraying process to mix the enzyme solution evenly;

[0042] The complex enzyme solution is an aqueous solution of a complex enzyme preparation, and the pH value is adjusted to 4-7 by using 1 mol / L citric acid solution; specifically, the pH value may be 4, 5, 6, or 7.

[0043] The mass concentration of the complex enzyme solution is 3wt% to 6wt%; specifically, it can be 3wt%, 4wt%, 5wt%, 6wt%; or a range between any two of the above.

[0044] The laccase activity is 10,000 to 20,000 U / g; the pectinase activity is 10,000 to 15,000 U / g; and the protease activity is 10,000 to 15,000 U / g.

[0045] The composite enzyme of the present invention comprises one or more of laccase, pectinase and protease; more preferably, the composite enzyme comprises laccase, pectinase and protease; the mass ratio of the laccase, pectinase and protease is (1-3):(0-1):(0-1); more preferably, the mass ratio of the laccase, pectinase and protease is (1-3):1:1.

[0046] In some embodiments, the mass ratio of laccase, pectinase and protease is 1:1:1;

[0047] In some embodiments, the mass ratio of laccase, pectinase and protease is 2:1:1;

[0048] In some embodiments, the mass ratio of laccase, pectinase and protease is 3:1:1.

[0049] The enzymatic hydrolysis temperature (i.e., the feeding cylinder temperature) of the present invention is 35-60°C, specifically 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, or any range between any two of the above.

[0050] The enzymatic hydrolysis time is 1 to 3 hours; specifically, it can be 1 hour, 2 hours, 3 hours; or a range between any two of the above.

[0051] After the enzymatic hydrolysis was completed, the temperature of the feeding barrel was raised to 90°C and the enzyme was inactivated for 10 minutes.

[0052] The added amount of the composite enzyme of the present invention is 5wt% to 8wt% of the steam-exploded material, specifically 5wt%, 6wt%, 7wt%, 8wt% or any range between any two of the above.

[0053] The present invention uses a biological composite enzyme to treat the tobacco stem material after steam explosion. The laccase in the composite enzyme is a copper-containing polyphenol oxidase that can oxidize phenolic compounds and decompose phenolic lignin. The tobacco stem material after steam explosion still contains a large amount of phenolic lignin. The use of laccase can effectively degrade the lignin therein, reducing the irritation and impurities of the tobacco stem; the pectinase and protease in the composite enzyme can degrade the pectin and protein in the material to generate amino acids and polypeptides, and the amino acids and polypeptides release some aroma substances such as sugar alcohols, organic acids, and phenols; the above three enzymes act synergistically to achieve a better effect.

[0054] The enzymatically hydrolyzed material is subjected to flash extraction. The enzymatically hydrolyzed tobacco stems, tobacco scraps and added fibers are mixed according to the formula ratio and put into a flash extractor, using water as the solvent;

[0055] The parameters of the flash extraction are: extraction time 1 to 3 minutes, material-liquid mass ratio 1:(4 to 6), extraction voltage 120 to 160V.

[0056] The extraction time is 1 min, 2 min or 3 min; or a range between any two of the above.

[0057] The mass ratio of the feed to the liquid is 1:4, 1:5, 1:6; or a range value between any two of the above.

[0058] The extraction voltage is 120V, 130V, 140V, 150V or 160V.

[0059] In some embodiments, the process conditions are extraction time 2 min, feed-liquid mass ratio 1:4, and extraction voltage 160V.

[0060] After extraction, the mixture is filtered, concentrated, coated on a sheet base, dried, and cut to obtain a reconstituted tobacco leaf product. After extraction, the mixture is filtered with gauze, the residue is pulped and sheeted, the liquid is refined and concentrated, flavoring is added, and then coated on a sheet base, dried, and cut to obtain a reconstituted tobacco leaf product.

[0061] The concentration parameters of the present invention include: concentration temperature of 50-60°C, concentration to liquid solid content of 38-42%; the drying parameters include drying temperature of 100-110°C, drying to material moisture of 10-12%.

[0062] The present invention does not limit the above-mentioned steps of coating, slitting, etc., which are well known to those skilled in the art.

[0063] The present invention provides a papermaking method reconstituted tobacco leaf, which is prepared by the preparation method described in any one of the above technical solutions.

[0064] The present invention has clearly described the above preparation method, which will not be repeated here.

[0065] The present invention provides a tobacco product, comprising the reconstituted tobacco leaves described in the above technical solution.

[0066] In the following examples, "smoking article" refers to any product that can be smoked, whether based on tobacco, tobacco derivatives, expanded tobacco, reconstituted tobacco or tobacco substitutes, or any product that can provide a smoking experience. For example, smoking articles may include smokable articles such as cigarettes, cigars and cigarillos. As another example, smoking articles may include combustion smoking articles and heat smoking articles.

[0067] Tobacco product can comprise for example tobacco material.Tobacco material can comprise for example tobacco leaf, tobacco stem or by the material etc. of its processing.As more specific example, tobacco material can comprise the tobacco leaf of pulverizing, the reconstituted tobacco of pulverizing, the stem and lobule of expansion etc.But the present invention is not limited to this.In addition, tobacco material can have but not limited to the form of tobacco shred filler, tobacco particle, tobacco sheet, tobacco pearl, tobacco particle or tobacco extract.

[0068] The present invention firstly processes tobacco stems by steam explosion, thereby reducing the content of chemical components such as total cell wall materials and hemicellulose of tobacco stems, and increasing the content of aroma components such as Maillard reactants and the total amount of neutral aroma substances of tobacco stems; then, the tobacco stem material after steam explosion is treated with a biological composite enzyme, wherein the laccase in the composite enzyme is a copper-containing polyphenol oxidase, which can oxidize phenolic compounds and decompose phenolic lignin. The tobacco stem material after steam explosion still contains a large amount of phenolic lignin, and the laccase can effectively degrade the lignin therein, thereby reducing the irritation and impurity of the tobacco stems; the pectinase and protease in the composite enzyme can degrade the pectin and protein in the material, generate amino acids and polypeptides, and the amino acids and polypeptides release some aroma substances such as sugar alcohols, organic acids, and phenols; finally, the tobacco stems, tobacco scraps, and the added fiber mixture after enzymolysis are extracted by flash extraction, and the flash extraction does not require heating, thereby effectively protecting the heat-sensitive components in the material; the original extraction work of 40 minutes or more can be completed in just a few minutes by the flash extraction, and the work efficiency is high and the extraction is more complete. When the flash extractor is working, the inner knife is driven by a high-speed motor and its speed is adjusted by the control system. It rotates at high speed in the outer knife chamber, so that the whole system is in a high-speed dynamic environment. Not only a strong shearing effect is generated between the inner and outer knives, but also a strong negative pressure is generated in the outer knife chamber. Under the action of this negative pressure, molecular penetration occurs inside and outside the outer knife chamber, that is, the substance molecules (extracted components) that are fully exposed by breaking are surrounded, dissociated, dissolved, replaced, and separated by solvent molecules under the action of various external forces such as negative pressure, shear, and high-speed collision, and then quickly enter the solvent, instantly reaching a balance of dissolved concentration, and the extraction process can be completed in a very short time.

[0069] It should be understood that the order of steps or the order in which certain actions are performed is not important as long as the present invention remains operable. In addition, two or more steps or actions may be performed simultaneously.

[0070] The use of any and all examples or exemplary language, such as "for example" or "including", herein is intended only to better illustrate the invention and does not limit the scope of the invention unless otherwise claimed. No language in this specification should be construed as indicating that any non-claimed element is essential to the practice of the invention.

[0071] In addition, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as accurately as possible. However, any numerical value inherently inevitably contains standard deviations due to individual test methods. Therefore, unless otherwise expressly stated, it should be understood that all ranges, quantities, values ​​and percentages used in this disclosure are modified by "about". Here, "about" generally means that the actual value is within plus or minus 10%, 5%, 1% or 0.5% of a specific value or range.

[0072] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0073] Some cases are recorded in the embodiments and comparative examples of the present invention, wherein the embodiments show certain implementation modes of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases.

[0074] In order to further illustrate the present invention, a preparation method and application of improving the quality of reconstituted tobacco leaves by papermaking process provided by the present invention are described in detail below in combination with embodiments.

[0075] Example 1

[0076] A preparation method for improving the sensory quality of papermaking reconstituted tobacco, comprising the following steps:

[0077] (1) Steam explosion of tobacco stem raw materials: tobacco stems are placed into a vertical high-pressure sample introduction cylinder of a steam explosion device, saturated water vapor is introduced until the steam pressure reaches 1.0 MPa, and the pressure maintenance time is controlled at 60 seconds to allow the raw materials to be fully infiltrated, and then the discharge valve is quickly opened to discharge the materials into a storage tank, and then the materials are transferred to a feeding drum;

[0078] (2) Pre-treating the steam-exploded material obtained in step (1) with a biological composite enzyme: spraying the composite enzyme solution onto the material, rotating the feeding barrel during the spraying process to mix the enzyme solution evenly, controlling the feeding barrel temperature at 50° C. for 1 h; spraying the composite enzyme solution at a ratio of 5% of the mass of the smoke of the exploded material; the composite enzyme solution is prepared from a composite enzyme preparation and pure water, and adjusted to pH 5 with a 1 mol / L citric acid solution, and the mass concentration of the composite enzyme solution is 6%; the composite enzyme preparation is composed of laccase, pectinase and protease, and the mass ratio of the components is 2:1:1, wherein the activities of laccase, pectinase and protease are 20,000 U / g, 15,000 U / g and 10,000 U / g, respectively; after the enzymolysis is completed, the feeding barrel temperature is raised to 90° C., and the enzyme is inactivated for 10 min;

[0079] (3) Mixing the materials by flash extraction: The enzymatically hydrolyzed tobacco stems, tobacco scraps and added fibers obtained in step (2) are mixed according to a formula ratio and placed in a flash extractor, with water as the solvent. The process conditions are extraction time 2 min, material-liquid mass ratio 1:4, and extraction voltage 160 V.

[0080] (4) After the extraction is completed, the mixed material obtained in step (3) is filtered with gauze, the residue is pulped and sheeted, the liquid material is refined and concentrated, and after adding flavoring, it is coated on the sheet base, dried and cut to obtain the reconstituted tobacco leaf product.

[0081] Comparative Example 1

[0082] This comparative example provides a papermaking process reconstituted tobacco leaf, and the preparation method is as follows:

[0083] (1) Material preparation: prepare tobacco stems and tobacco shreds in a mass ratio of 4:6; prepare additional fiber in a mass ratio of 8% of tobacco raw materials (coniferous wood pulp: hardwood wood pulp = 3:2);

[0084] (2) Tobacco stem extraction: The tobacco stems of step (1) were extracted at a material ratio of 1:4 to water, the extraction time was 40 min, and the extraction temperature was 70°C. After the extraction, solid-liquid separation was performed, and the tobacco stem extract and stem material were obtained after defibration.

[0085] (3) Mixed extraction: the tobacco fragments and added fibers of step (1) and the stem material of step (2) are mixed in a high-consistency pulper, and extracted at a material ratio of 1:7 to water, for 30 minutes at a temperature of 70° C. After the extraction, solid-liquid separation is performed, and water in the residue is removed by a squeezer to obtain a tobacco residue extract and a residue;

[0086] (4) Pulping: The residue extracted in step (3) is subjected to low-concentration grinding to obtain slurry.

[0087] (5) Refining and concentration: the tobacco stem extract and the tobacco dust extract obtained in steps (2 and 3) are mixed in a ratio of 2:8, subjected to membrane treatment, evaporated and concentrated, and 0.5% of flavors and fragrances are added to obtain a coating liquid.

[0088] (6) Papermaking: The slurry prepared in step (4) that meets the papermaking requirements is evenly transported to the forming net at a sizing concentration of 1.0%. The slurry on the forming net is dehydrated to a certain dryness, preliminarily formed, and then pressed in a large cylinder and dried in multiple cylinders to obtain a sheet base.

[0089] (7) Coating and post-processing: The coating liquid obtained in step (5) is evenly coated on the sheet base prepared in step (6), and the reconstituted tobacco leaf product is obtained through processes such as oven drying, slitting, drying drum drying, sheet storage, and packaging.

[0090] According to the calculation of the proportion of soluble matter in the residue and reconstituted tobacco product in Example 1 and Comparative Example 1, the extraction rates of soluble matter in the reconstituted tobacco product were 94.6% and 90.2%, respectively, and the soluble matter in the example was extracted more completely.

[0091] Comparative Example 2

[0092] This comparative example provides a papermaking process reconstituted tobacco leaf, and the preparation method is as follows:

[0093] (1) Material preparation: prepare tobacco stems and tobacco shreds in a mass ratio of 4:6; prepare additional fiber in a mass ratio of 8% of tobacco raw materials (coniferous wood pulp: hardwood wood pulp = 3:2);

[0094] (2) Tobacco stem extraction: The tobacco stems of step (1) were extracted at a material ratio of 1:4 to water, the extraction time was 40 minutes, the extraction temperature was 70°C, solid-liquid separation was performed after extraction, and tobacco stem extract and stem material were obtained after defibration. 10u / g α-amylase, 100u / g saccharifying enzyme, and 3000u / g pectinase were added to the tobacco stem extract, and citric acid was added to adjust the pH to 6.5. The reaction was carried out at 75°C and pH 6.5 for 3 hours to obtain the tobacco stem extract after enzymatic hydrolysis.

[0095] (3) Mixed extraction: the tobacco fragments and added fibers of step (1) and the stem material of step (2) are mixed in a high-consistency pulper, and extracted at a material ratio of 1:7 to water, for 30 minutes at a temperature of 70° C. After the extraction, solid-liquid separation is performed, and water in the residue is removed by a squeezer to obtain a tobacco residue extract and a residue;

[0096] (4) Pulping: The residue extracted in step (3) is subjected to low-concentration grinding to obtain slurry.

[0097] (5) Refining and concentration: the enzymatically hydrolyzed tobacco stem extract and the tobacco dust extract obtained in steps (2 and 3) are mixed in a ratio of 2:8, subjected to membrane treatment, evaporated and concentrated, and 0.5% of flavors and fragrances are added to obtain a coating solution.

[0098] (6) Papermaking: The slurry prepared in step (4) that meets the papermaking requirements is evenly transported to the forming net at a sizing concentration of 1.0%. The slurry on the forming net is dehydrated to a certain dryness, preliminarily formed, and then pressed in a large cylinder and dried in multiple cylinders to obtain a sheet base.

[0099] (7) Coating and post-processing: The coating liquid obtained in step (5) is evenly coated on the sheet base prepared in step (6), and the reconstituted tobacco leaf product is obtained through processes such as oven drying, slitting, drying drum drying, sheet storage, and packaging.

[0100] According to the calculation of the proportion of soluble matter in the residue and reconstituted tobacco product in Example 1 and Comparative Example 1, the extraction rates of soluble matter in the reconstituted tobacco product were 94.6% and 90.2%, respectively, and the soluble matter in the example was extracted more completely.

[0101] Comparative Example 3

[0102] (1) Steam explosion of tobacco stem raw materials: tobacco stems are placed into a vertical high-pressure sample introduction cylinder of a steam explosion device, saturated water vapor is introduced until the steam pressure reaches 1.0 MPa, and the pressure maintenance time is controlled at 60 seconds to allow the raw materials to be fully infiltrated, and then the discharge valve is quickly opened to discharge the materials into a storage tank, and then the materials are transferred to a feeding drum;

[0103] (2) Pre-treating the steam-exploded material obtained in step (1) with a biological composite enzyme: spraying the composite enzyme solution onto the material, rotating the feeding barrel during the spraying process to mix the enzyme solution evenly, controlling the feeding barrel temperature at 50° C. for 1 h; spraying the composite enzyme solution at a ratio of 5% of the mass of the smoke of the exploded material; the composite enzyme solution is prepared from a composite enzyme preparation and pure water, and adjusted to pH 5 with a 1 mol / L citric acid solution, and the mass concentration of the composite enzyme solution is 5%; the composite enzyme preparation is composed of pectinase and protease, and the mass ratio of the components is 1:1, wherein the pectinase and protease activities are 15,000 U / g and 10,000 U / g, respectively; after the enzymolysis is completed, the feeding barrel temperature is raised to 90° C., and the enzyme is inactivated for 10 min;

[0104] (3) Mixing the materials by flash extraction: The enzymatically hydrolyzed tobacco stems, tobacco scraps and added fibers obtained in step (2) are mixed according to a formula ratio and placed in a flash extractor, with water as the solvent. The process conditions are extraction time 2 min, material-liquid mass ratio 1:4, and extraction voltage 160 V.

[0105] (4) After the extraction is completed, the mixed material obtained in step (3) is filtered with gauze, the residue is pulped and sheeted, the liquid material is refined and concentrated, and after adding flavoring, it is coated on the sheet base, dried and cut to obtain the reconstituted tobacco leaf product.

[0106] Verification Example 1

[0107] The different samples were cut into shreds and rolled into cigarettes. After balancing at 22±2℃ and 60±5% humidity for 48 hours, a seven-member panel conducted a sensory evaluation. The results are as follows:

[0108]

[0109] It can be found from the above data that the smoking evaluation panel believes that the reconstituted tobacco product provided by the present invention has the advantages of less impurities, strong aroma, sufficient smoke flavor and good smoking effect.

[0110] Verification Example 2

[0111] The soluble matter extraction rate index of tobacco raw materials is implemented according to the detection method in Appendix D of YC / T 584-2019 "Technology Specifications for Reconstituted Tobacco Leaves". After calculation, the soluble matter extraction rates of raw materials in Example 1 and Comparative Examples 1, 2, and 3 are 94.6%, 90.2%, 90.8%, and 91.3%, respectively. The soluble matter extraction of tobacco raw materials in the embodiments is more complete.

[0112] The lignin content was determined using the standard method of YC / T 347-2010 “Determination of Neutral Detergent Fiber, Acid Detergent Fiber and Acid-Washed Lignin in Tobacco and Tobacco Products - Detergent Method”.

[0113] The method for determining the content of aroma components is as follows:

[0114] The aroma components in the sample were determined by simultaneous distillation and extraction. 20.00 g of the sample (Example 1 and Comparative Examples 1, 2, and 3) was weighed and placed in a 500 mL two-necked round-bottom flask, and 20 g of sodium chloride and 250 mL of distilled water were added. Another 50 mL of dichloromethane was placed in a 250 mL round-bottom flask, and 0.10 mL of 1 mg / mL phenylethyl acetate internal standard solution was added. Simultaneous distillation and extraction were performed for 3 h, anhydrous sodium sulfate was added to the dichloromethane solution and dried for at least 2 h, and concentrated to 1 mL at 40° C. under normal pressure for analysis.

[0115] GC / MS conditions: HP-5MS capillary column (30.00m*0.25mm*0.25μm); high-purity helium, flow rate 1.0mL / min; injection port temperature 250℃; injection volume 1μL, split ratio 10:1. Program temperature rise: initial temperature 50℃, hold for 2min, increase to 200℃ at 3℃ / min, hold for 2min, then increase to 260℃ at 6℃ / min, hold for 5min.

[0116] MS: transmission line temperature 280℃; ion source temperature 230℃; quadrupole temperature 150℃; electromagnetic source EI; ionization energy 70ev; mass number 33~550amu; mass spectrometry scanning mode is full scan; solvent delay 3.5min. MS library: according to NIST11 library search, qualitative analysis with a matching degree ≥80%, assuming a relative correction factor of 1, and internal standard method for quantitative analysis.

[0117] The test results of Example 1 and Comparative Examples 1, 2, and 3 are as follows:

[0118]

[0119]

[0120]

[0121] It can be seen from the table that compared with the comparative example, the contents of heat-sensitive substances such as benzyl alcohol, damascena, megastigmatrienone 2, furfural, solanone, neophytadiene, dihydroactin, ethyl hexadecanoate, etc. in the embodiments of the present invention are significantly increased.

[0122] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A preparation method for improving the quality of papermaking reconstituted tobacco, characterized in that: The steps include: A) steam-exploding a tobacco stem raw material to obtain a steam-exploded material; B) pretreating the steam-exploded material with a composite enzyme solution to obtain an enzymatically hydrolyzed material; the composite enzyme comprises one or more of laccase, pectinase and protease; C) The enzymatically hydrolyzed material is subjected to flash extraction, and then filtered, concentrated, coated onto a sheet base, dried, and cut to obtain a reconstituted tobacco leaf product.

2. The preparation method according to claim 1, characterized in that: The parameters of the steam explosion in step A) include: saturated steam pressure of 0.8-1.2 MPa, and pressure maintenance time of 30-90 s.

3. The preparation method according to claim 1, characterized in that: Step B) specifically comprises: spraying the composite enzyme solution onto the steam-exploded material, and during the spraying process, rotating the feeding cylinder in the steam explosion device to mix the enzyme solution evenly; The complex enzyme solution is an aqueous solution of a complex enzyme preparation, and the pH value is adjusted to 4-7 by using 1 mol / L citric acid solution; the mass concentration of the complex enzyme solution is 3wt%-6wt%.

4. The preparation method according to claim 1, characterized in that: Step B) the mass ratio of laccase, pectinase and protease is (1-3):(0-1):(0-1); The laccase activity is 10,000 to 20,000 U / g; the pectinase activity is 10,000 to 15,000 U / g; and the protease activity is 10,000 to 15,000 U / g.

5. The preparation method according to claim 1, characterized in that: Step B) The enzymolysis temperature is 35-60°C for 1-3 hours; after the enzymolysis is completed, the temperature is raised to 90°C and the enzyme is inactivated for 10 minutes.

6. The preparation method according to claim 1, characterized in that: Step B) The added amount of the complex enzyme is 5wt% to 8wt% of the steam-exploded material.

7. The preparation method according to claim 1, characterized in that: Step C) The parameters of the flash extraction are: extraction time 1-3 min, material-liquid mass ratio 1:(4-6), extraction voltage 120-160V.

8. The preparation method according to claim 1, characterized in that: Step C) The filtration is performed through gauze; the concentration parameters include: a concentration temperature of 50-60°C, and concentration to a liquid solid content of 38-42%; the drying parameters include a drying temperature of 100-110°C, and drying to a material moisture content of 10-12%.

9. A papermaking process reconstituted tobacco leaf, characterized in that: The method is prepared according to any one of claims 1 to 9.

10. A tobacco product, characterized in that: Including the reconstituted tobacco leaf as described in claim 9.

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