Emotional cosmetic composition for sensitive skin and its preparation method and application

Abstract

The application discloses a mood beauty composition suitable for sensitive skin and a preparation method and application thereof, and relates to the technical field of cosmetics.The composition comprises the following components in parts by weight: Copaiba Balsam Resin 0.08-6 parts, acetyl tetrapeptide-15 0.005-2 parts, Albizia Lebbeck Seed Extract 0.06-6 parts, Citrus Fruits Extract 0.008-3 parts, and Schizonepeta Extract 0.008-4 parts.The application combines Copaiba Balsam Resin, acetyl tetrapeptide-15, Albizia Lebbeck Seed Extract, Citrus Fruits Extract and Schizonepeta Extract to find a synergistic effect, and the composition formed by the specific combination of the components can reduce the skin damage caused by the resistance to emotional fluctuations, relieve stress, bring a pleasant feeling to the skin, improve the oil secretion and sensitive condition of the skin from the inside to the outside, achieve the effects of pleasure, oil control and soothing, and is particularly suitable for oily and sensitive skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an emotional beauty composition suitable for sensitive skin, its preparation method, and its application. Background Technology

[0002] Based on research in skin neurophysiology, the beauty industry has proposed concepts such as "neuro-cosmetics," "emotional beauty," and "emotional skincare" to describe beauty methods that improve skin health and appearance by regulating emotional states. The principle is based on the influence of emotions on bodily physiology, particularly by reducing negative emotions such as stress and anxiety, promoting hormonal balance and blood circulation, thereby improving skin condition.

[0003] In the field of oily and sensitive skin care, the concept of emotional beauty has garnered particular attention. Emotional states, such as stress and anxiety, not only directly affect sebum secretion but also indirectly influence sebaceous gland function by activating stress responses in the body (such as increasing cortisol secretion), thus exacerbating oily and sensitive skin problems. Existing research shows that emotions have a significant impact on skin health; negative emotions can damage the skin barrier function, causing an imbalance of oil and water, further aggravating oiliness and sensitivity symptoms. Therefore, emotional beauty, by regulating emotional state and reducing stress, improves skin health, becoming a new skincare trend. Combining the concept of emotional beauty can effectively address the shortcomings of simple oil-control products in fundamentally alleviating oily and sensitive skin problems, providing comprehensive conditioning for the skin.

[0004] Currently available oil-control products typically focus only on reducing sebum secretion, neglecting the skin's sensitive reactions caused by stress and negative emotions. Therefore, many oil-control products lack soothing effects and may not fundamentally address the problem of excess oil production caused by emotional fluctuations. Furthermore, existing oily and sensitive skin care products rarely address mechanisms that improve skin condition through mood or stress reduction. Even those products containing soothing ingredients usually focus on localized skin calming without truly considering the long-term effects of emotions and stress on the skin.

[0005] Existing oil-control products typically focus only on reducing sebum secretion, neglecting the skin's sensitivity reactions caused by stress and negative emotions. Current soothing ingredients generally emphasize localized skin calming, without truly considering the long-term effects of emotions and stress on the skin. Therefore, finding emotionally-driven beauty products suitable for oily and sensitive skin has become a pressing technical problem to be solved in this field. Summary of the Invention

[0006] Based on this, the purpose of the present invention is to overcome the shortcomings of the prior art and provide an emotional beauty composition and its application suitable for oily and sensitive skin. The composition can reduce and combat skin damage caused by emotional fluctuations, relieve stress, bring pleasure to the skin, improve the oil production and sensitivity of the skin from the inside out, and achieve the effects of pleasure, oil control and soothing. It is especially suitable for people with oily and sensitive skin.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides an emotional beauty composition comprising the following components in parts by weight: 0.08-6 parts of Cuban gum resin, 0.005-2 parts of acetyl tetrapeptide-15, 0.06-6 parts of grey soybean seed extract, 0.008-3 parts of citrus fruit extract, and 0.008-4 parts of catnip extract.

[0009] The Cuban balsam resin (COPAIFERA OFFICINALIS) is a resin extracted from the Cuban balsam tree. Rich in β-caryophyllene, it can act on the skin's CB2 receptors, promoting the release of the "happy hormone" β-endorphin, thus achieving a soothing and calming effect on the skin. The acetyl tetrapeptide-15 is a specific and potent μ-opioid receptor agonist. μ-opioid receptors are one of the main receptors for β-endorphin in the body; therefore, acetyl tetrapeptide-15 can mimic the pleasurable sensations induced by β-endorphin on the skin. The edamame (TEPHROSIA PURPUREA) seed extract is an extract from the seeds of the edamame plant, rich in polysaccharides and flavonoid glycosides. It relieves skin stress by lowering levels of the stress hormone cortisol. The Citrus Reticulata fruit extract is a natural product extracted from citrus fruits. Rich in polyphenols and flavonoids, it possesses powerful antioxidant properties, effectively combating skin damage caused by emotional stress. The natural aroma of citrus also helps alleviate mood swings, providing a pleasant psychological experience and promoting relaxation and mental well-being. The Nepeta Cataria extract is a natural product extracted from the leaves of the Nepeta cataria plant. Containing volatile oils and tannins, it can regulate sebum secretion and reduce the negative emotions associated with oiliness.

[0010] This invention has found that when the above-mentioned plant extracts are combined with specific amounts of Cuban balsamic gum resin and acetyl tetrapeptide-15, they can synergistically reduce skin damage caused by emotional fluctuations, relieve stress, and bring a sense of pleasure to the skin. They can improve the oiliness and sensitivity of the skin from the inside out, achieving a pleasant, oil-controlling, and soothing effect, and are especially suitable for people with oily and sensitive skin.

[0011] As a preferred embodiment of the first aspect, the mass ratio of the sum of Cuban balsam resin and acetyl tetrapeptide-15, the sum of the mass of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract in the composition is: (Cuban balsam resin + acetyl tetrapeptide-15): (Soybean seed extract + citrus fruit extract): Catnip extract = (0.11-6):(0.11-7):(0.01-3). Exemplarily, the mass ratio of the sum of Cuban balsam resin and acetyl tetrapeptide-15, the sum of the mass of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract can be any value or a range between any of the following: 0.11:0.11:0.01, 1:2:0.5, 3:4:0.6, 5:5:2, 6:7:3.

[0012] As a preferred embodiment of the first aspect, the mass ratio of the sum of the Cuban balsam resin and acetyl tetrapeptide-15, the sum of the mass of the sum of the mass of the gray soybean seed extract and the citrus fruit extract, and the mass ratio of the catnip extract in the composition is: (Cuban balsam resin + acetyl tetrapeptide-15): (gray soybean seed extract + citrus fruit extract): catnip extract = (0.55-3.5): (0.3-5.5): (0.1-1).

[0013] As a preferred embodiment of the first aspect, the mass ratio of the sum of Cuban balsam resin and acetyl tetrapeptide-15, the sum of the mass of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract in the composition is: (Cuban balsam resin + acetyl tetrapeptide-15): (Soybean seed extract + citrus fruit extract): Catnip extract = 1.2:2.8:0.5.

[0014] This invention has found that the mass ratio of Cuban balsam resin and acetyl tetrapeptide-15, the mass ratio of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract in the emotional beauty composition not only affects the release of β-endorphin and cortisol levels in the human body, thus influencing the composition's soothing, calming, mood-improving effects and the pleasant feeling it brings to the skin, but also reduces the body's ability to regulate sebum secretion, thereby affecting the composition's oil-controlling effect. When the mass ratio of Cuban balsam resin and acetyl tetrapeptide-15, the mass ratio of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract are further selected to (0.11-6):(0.11-7):(0.01-3), especially (0.55-3.5):(0.3-5.5):(0.1-1), the resulting emotional beauty composition exhibits superior soothing and calming effects, mood-improving effects, and the pleasant feeling it brings to the skin; it also significantly enhances the composition's oil-controlling effect, improving its overall comprehensive capabilities in oil control, soothing, calming, and mood improvement. Studies have shown that the overall effect of the composition of the present invention is optimal when the mass ratio of the combined mass of Cuban balsam resin and acetyl tetrapeptide-15, the combined mass ratio of the combined mass of soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract is 1.2:2.8:0.5.

[0015] In a preferred embodiment of the first aspect, the mass ratio of Cuban balsamic resin to acetyl tetrapeptide-15 in the composition is Cuban balsamic resin:acetyl tetrapeptide-15 = (0.1-5):(0.01-1). Exemplarily, the mass ratio of Cuban balsamic resin to acetyl tetrapeptide-15 can be any value or a range between 0.1:0.01, 1:0.02, 3:0.05, 5:0.08, and 5:1.

[0016] In a preferred embodiment of the first aspect, the mass ratio of Cuban balsamic resin to acetyl tetrapeptide-15 in the composition is Cuban balsamic resin:acetyl tetrapeptide-15 = (0.5-3):(0.05-0.5).

[0017] In a preferred embodiment of the first aspect, the composition contains a Cuban balsamic resin and acetyl tetrapeptide-15 in a mass ratio of Cuban balsamic resin:acetyl tetrapeptide-15 = 1:0.2.

[0018] This invention has found that the mass ratio of guacamole resin to acetyl tetrapeptide-15 in the mood-enhancing composition can affect the composition's effects on β-endorphin release and inhibition of the stress hormone cortisol, thereby influencing the composition's ability to improve negative emotions and provide soothing and calming effects. Further optimization of the mass ratio of guacamole resin to acetyl tetrapeptide-15 to (0.1-5):(0.01-1), especially (0.5-3):(0.05-0.5), can enhance the composition's β-endorphin release, inhibition of the stress hormone cortisol, and improve SAM scale scores, a* improvement rate, and oil content improvement rate, thereby enhancing the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production. The study found that the composition of this invention exhibits the best effect when the mass ratio of guacamole resin to acetyl tetrapeptide-15 is 1:0.2.

[0019] In a preferred embodiment of the first aspect, the mass ratio of edamame seed extract to citrus fruit extract in the composition is edamame seed extract:citrus fruit extract = (0.1-5):(0.01-2). Exemplarily, the mass ratio of edamame seed extract to citrus fruit extract can be any value or a range of values ​​from 0.1:0.01, 0.5:0.08, 0.8:0.1, 1:0.5, 3:1, 5:2.

[0020] In a preferred embodiment of the first aspect, the mass ratio of soybean seed extract to citrus fruit extract in the composition is soybean seed extract:citrus fruit extract = (0.2-4):(0.1-1.5).

[0021] In a preferred embodiment of the first aspect, the mass ratio of edamame seed extract to citrus fruit extract in the composition is edamame seed extract: citrus fruit extract = 1.8:1.

[0022] This invention has found that the mass ratio of soybean seed extract to citrus fruit extract in the mood-enhancing composition can, to a certain extent, affect the composition's ability to release β-endorphins and inhibit the level of the stress hormone cortisol, as well as its ability to improve SAM scale scores, a* improvement rate, and oil content improvement rate. This, in turn, affects the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production. Furthermore, when the mass ratio of soybean seed extract to citrus fruit extract is further selected to be (0.1-5):(0.01-2), especially (0.2-4):(0.1-1.5), the overall efficacy of the composition can be enhanced. The study found that the composition of this invention exhibits the best effect when the mass ratio of soybean seed extract to citrus fruit extract is 1.8:1.

[0023] As a preferred embodiment of the first aspect, the preparation method of the grey soybean seed extract includes the following steps: drying the seeds of grey soybean, pulverizing them, adding them to an aqueous solution of a eutectic solvent, ultrasonically extracting them, collecting the product, centrifuging them, and obtaining the grey soybean seed extract.

[0024] As a preferred embodiment of the first aspect, the method for preparing the edamame seed extract satisfies at least one of the following:

[0025] (a) The eutectic solvent is composed of betaine and oxalic acid in a mass ratio of betaine:oxalic acid = 1:(1-3);

[0026] (b) The mass concentration of the eutectic solvent in the aqueous solution of the eutectic solvent is 20-60%;

[0027] (c) The temperature of the ultrasonic extraction is 40-60℃; or / and the power of the ultrasonic extraction is 150-200W, and the time of the ultrasonic extraction is 20-50min;

[0028] (d) The material-to-liquid ratio for ultrasonic extraction is 1g:(20-40)mL.

[0029] This invention has found that different extraction methods affect the extract of soybean seeds and the synergistic effect of the extract with other components, thereby affecting the composition's ability to inhibit the stress hormone cortisol levels and increase a* value, thus affecting the composition's soothing and sedative effects.

[0030] In a second aspect, the present invention provides the use of the mood-enhancing composition as described in the first aspect in the preparation of cosmetics.

[0031] As a preferred embodiment of the second aspect, the cosmetic includes one or more of the following: lotion, cream, mask, serum, and spray.

[0032] Thirdly, the present invention provides an emulsion comprising the following components by weight percentage: 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH adjuster, 0.5-5% emulsifier, 0.5-3% preservative, 1-10% of the aforementioned mood-enhancing composition, and the balance being water.

[0033] As a preferred embodiment of the third aspect, the emulsifier includes at least one of C12-20 alkyl glucoside, caprylic / capric triglyceride, and C14-22 alcohol; and / or, the preservative includes at least one of 1,2-hexanediol and p-hydroxyacetophenone; and / or, the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high molecular weight cellulose; and / or, the humectant includes at least one of glycerin, butylene glycol, low molecular weight sodium hyaluronate, allantoin, sodium polyacrylate, and hydrogenated lecithin; and / or, the pH adjuster includes at least one of arginine and disodium EDTA.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] This invention discovers that Cuban balsam resin, acetyl tetrapeptide-15, edamame seed extract, citrus fruit extract, and catnip extract have a synergistic effect from numerous plant extracts and raw materials. The combination formed by their specific formulation can reduce and combat skin damage caused by emotional fluctuations, relieve stress, and bring a sense of pleasure to the skin. It improves the oiliness and sensitivity of the skin from the inside out, achieving a pleasant, oil-controlling, and soothing effect, and is especially suitable for people with oily and sensitive skin. Attached Figure Description

[0036] Figure 1 This is a comparison chart showing the improvement of the red zone in the human efficacy test before and after use in volunteers in the blank application example and application example 18 of this invention. Detailed Implementation

[0037] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific embodiments. The purpose is to provide a detailed understanding of the invention, not to limit it. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. Unless otherwise specified, the experimental reagents and instruments designed in the embodiments and comparative examples of this invention are commonly used reagents and instruments, all of which are commercially available. Unless otherwise specified, the experimental methods used in the embodiments and comparative examples are conventional methods; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch. Further explanation of the raw materials used in this invention:

[0038] Cuban balsam resin: Product name is Cuban balsam essential oil, purchased from Guangzhou Fuyicheng Biotechnology Co., Ltd.

[0039] Acetyl tetrapeptide-15: Product code hx201900043, purchased from Xi'an Huaxing Biotechnology Co., Ltd.;

[0040] Citrus fruit extract: Product code HX-353, purchased from Guangzhou Hongxin Chemical Co., Ltd.;

[0041] Catnip extract: Product name HX-666, purchased from Guangzhou Hongxin Chemical Co., Ltd.;

[0042] Benzoin resin extract: The product name is benzoin essential oil, purchased from Jiangxi Juncai Biotechnology Co., Ltd.;

[0043] Acetyl hexapeptide-8: Product code HXSWYXJ-9, purchased from Xi'an Huaxing Biotechnology Co., Ltd.

[0044] Grape seed extract: Product code HX-235, purchased from Guangzhou Hongxin Chemical Co., Ltd.;

[0045] Fig extract: Product code HX-462, purchased from Guangzhou Hongxin Chemical Co., Ltd.;

[0046] Horsetail extract: Product name HX-575, purchased from Guangzhou Hongxin Chemical Co., Ltd.;

[0047] The preparation method of edamame seed extract-1 is as follows:

[0048] (1) Prepare a eutectic solvent:

[0049] Take 10g of betaine and 10g of oxalic acid respectively and dissolve them in 80g of deionized water to obtain an aqueous solution of eutectic solvent with a mass concentration of 20%.

[0050] (2) Preparation of grey soybean seed extract:

[0051] Seeds of *Edamame foetida* were collected, cleaned, and dried in a 50℃ constant temperature oven until constant weight. The seeds were then ground and passed through an 80-mesh sieve. 1g of the powder was added to an aqueous solution of the aforementioned eutectic solvent at a ratio of 1g:20mL. Ultrasonic extraction was performed at 40℃, 150W, and for 50 minutes. The product was collected, centrifuged, and the supernatant was taken to obtain *Edamame foetida* seed extract-1.

[0052] The preparation method of edamame seed extract-2 is as follows:

[0053] (1) Prepare a eutectic solvent:

[0054] Take 15g of betaine and 30g of oxalic acid and dissolve them in 55g of deionized water to obtain an aqueous solution of eutectic solvent with a mass concentration of 45%.

[0055] (2) Preparation of grey soybean seed extract:

[0056] Seeds of *Edamame foetida* were collected, cleaned, and dried in a 50°C oven until constant weight. The seeds were then ground and passed through an 80-mesh sieve. 1 g of the powder was added to an aqueous solution of the aforementioned eutectic solvent at a ratio of 1 g to 30 mL. Ultrasonic extraction was performed at 50°C, 180 W, and for 30 min. The product was collected, centrifuged, and the supernatant was taken to obtain *Edamame foetida* seed extract-2.

[0057] The preparation method of edamame seed extract-3 is as follows:

[0058] (1) Prepare a eutectic solvent:

[0059] Take 15g of betaine and 45g of oxalic acid respectively and dissolve them in 40g of deionized water to obtain an aqueous solution of eutectic solvent with a mass concentration of 60%.

[0060] (2) Preparation of grey soybean seed extract:

[0061] Seeds of *Edamame foetida* were collected, cleaned, and dried in a 50℃ constant temperature oven until constant weight. After grinding and pulverizing, the powder was passed through an 80-mesh sieve. 1g of the powder was added to an aqueous solution of the aforementioned eutectic solvent at a ratio of 1g:40mL. Ultrasonic extraction was performed at 60℃, 200W, and for 20 minutes. The product was collected, centrifuged, and the supernatant was taken to obtain *Edamame foetida* seed extract-3.

[0062] The preparation method of edamame seed extract-4 is as follows:

[0063] The seeds of grey soybean were collected, cleaned, and dried in a 50℃ constant temperature oven until constant weight. After grinding and pulverizing, the powder was passed through an 80-mesh sieve. 1g of the powder was added to a 75% ethanol aqueous solution at a material-to-liquid ratio of 1g:30mL. Ultrasonic extraction was performed at a temperature of 50℃, a power of 180W, and an extraction time of 30min. The product was collected, centrifuged, and the supernatant was collected to obtain the grey soybean seed extract-4.

[0064] The preparation method of edamame seed extract-5 is as follows:

[0065] The seeds of grey soybean were collected, cleaned, and dried in a 50℃ constant temperature oven until constant weight. After grinding and pulverizing, the powder was passed through an 80-mesh sieve. 1g of the powder was taken and pure water was added, with a material-to-liquid ratio of 1g:30mL. Ultrasonic extraction was performed at a temperature of 50℃, a power of 180W, and an extraction time of 30min. The product was collected, centrifuged, and the supernatant was taken to obtain the grey soybean seed extract-5.

[0066] The preparation method of the emotional beauty composition suitable for sensitive skin of the present invention is as follows: weigh each component by weight, stir and mix evenly, and sonicate for 10 minutes to obtain the emotional beauty composition.

[0067] Examples and Comparative Examples

[0068] This application provides an emotional beauty composition. The components and weight parts of the examples and comparative compositions are shown in Table 1.

[0069] Table 1:

[0070]

[0071]

[0072] Example of effect

[0073] This example demonstrates through cell experiments that the composition of the present invention can increase the release of the happiness hormone β-endorphin and reduce the release of the stress hormone cortisol, as detailed below:

[0074] Experimental methods:

[0075] The cell line used was human keratinocytes (HaCaT, Guangzhou Genio Biotechnology Co., Ltd.). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. Specifically, the levels of β-endorphin and cortisol were detected using the following methods:

[0076] (1) The cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of culture medium was added to each well. The cells were cultured for 24 h.

[0077] (2) Discard the supernatant. Add 100 μL of DMEM medium (2105341, Gibco) containing 1 μM hydrocortisone (Shanghai Yuanye Biotechnology, S31360) to the control group. Add 100 μL of medium containing 1 μM hydrocortisone and 0.5% of the corresponding sample to Examples 1-21 and Comparative Examples 1-19. Incubate for 24 h.

[0078] (3) Collect cells separately, wash 3 times with PBS buffer, add 50 μL of RIPA cell lysis buffer containing protease inhibitor to each well, centrifuge the lysed sample at 12000g for 5 minutes, and take the supernatant.

[0079] (4) β-Endorphin content determination: The β-endorphin content in the cell supernatant of each group was detected using a human β-endorphin ELISA kit (catalog number BLL102873E, Baililai Biotechnology).

[0080] (5) Cortisol content determination: The cortisol content of cell supernatant in each group was detected using a human cortisol ELISA kit (catalog number BLL107923E, Baililai Biotechnology).

[0081] This experiment expresses the improvement in emotional stress as the improvement rate of each value, calculated as follows:

[0082] β-Endorphin Increase Rate = (β-Endorphin) 样品组 -β-Endorphin 对照组 ) / β-Endorphin 对照组 ×100%;

[0083] Cortisol reduction rate = (cortisol) 对照组 - Cortisol 样品组 Cortisol 对照组 ×100%;

[0084] The higher the increase in β-endorphin and the lower the decrease in cortisol, the stronger the combination's ability to improve mood. The calculated results are shown in Table 2.

[0085] Table 2:

[0086]

[0087]

[0088] As can be seen from Table 2, the mood-enhancing composition provided by the present invention can increase β-endorphin levels and decrease cortisol levels, indicating that the composition of the present invention has the effect of improving negative emotions and soothing and calming.

[0089] As can be seen from Examples 1-17 and Comparative Examples 1-2, the mass ratio of the components in the mood-enhancing composition affects the composition's ability to release the happy hormone β-endorphin and inhibit the stress hormone cortisol, thereby affecting the overall mood-improving and calming effects of the composition.

[0090] Examples 1-7 show that the mass ratio of the components in the mood-enhancing composition affects the composition's ability to release the happy hormone β-endorphin and inhibit the stress hormone cortisol. When the mass ratio of the combined mass of Cuban gum resin and acetyl tetrapeptide-15, the combined mass of grey soybean seed extract and citrus fruit extract, and catnip extract in the composition is limited to the range of (0.11-6):(0.11-7):(0.01-3), the resulting composition has a good effect on improving negative emotions and soothing and calming. When the mass ratio is further selected to be in the range of (0.55-3.5):(0.3-5.5):(0.1-1), the resulting composition has an even better effect on improving negative emotions and soothing and calming. The best effect is achieved when the mass ratio is 1.2:2.8:0.5 (Example 7).

[0091] This invention investigated the effect of the mass ratio of guar gum resin and acetyl tetrapeptide-15 on the ability of the composition to release β-endorphin and inhibit the stress hormone cortisol through Examples 8-10. Examples 8-10 show that the mass ratio of guar gum resin and acetyl tetrapeptide-15 affects the composition's effect on β-endorphin release and inhibition of the stress hormone cortisol, thus influencing the composition's efficacy in improving negative mood and providing a calming effect. When the mass ratio of guar gum resin and acetyl tetrapeptide-15 is limited to the range of (0.1-5):(0.01-1), the composition's ability to release β-endorphin and inhibit the stress hormone cortisol is improved. Further optimization by limiting the mass ratio of guar gum resin and acetyl tetrapeptide-15 to the range of (0.5-3):(0.05-0.5) yields even better results. The optimal effect is achieved when the mass ratio of guar gum resin and acetyl tetrapeptide-15 is 1:0.2 (Example 8).

[0092] This invention investigated the effect of the mass ratio of soybean seed extract to citrus fruit extract on the composition's ability to release β-endorphins and inhibit the level of the stress hormone cortisol through Examples 11-13. Examples 11-13 show that the mass ratio of soybean seed extract to citrus fruit extract affects the composition's effect on releasing β-endorphins and inhibiting the level of the stress hormone cortisol, thus affecting the composition's efficacy in improving negative mood and providing a calming effect. When the mass ratio of soybean seed extract to citrus fruit extract is limited to the range of (0.1-5):(0.01-2), the composition's efficacy in improving negative mood and providing a calming effect is enhanced. Further optimization by limiting the mass ratio of soybean seed extract to citrus fruit extract to the range of (0.2-4):(0.1-1.5) yields even better results. The optimal effect is achieved when the mass ratio of soybean seed extract to citrus fruit extract is 1.8:1 (Example 12).

[0093] In summary, the optimal mass ratio of the components obtained in this invention is Cuban balsam resin: acetyl tetrapeptide-15: edamame seed extract: citrus fruit extract: catnip extract = 1:0.2:1.8:1:0.5 (Example 17).

[0094] This invention investigated the effects of different extraction methods of *Edamame foetida* seed extract on the composition's ability to release β-endorphin and inhibit the level of the stress hormone cortisol through Examples 18-21. Examples 18-21 show that the extraction method of *Edamame foetida* seed extract has a certain influence on the release of β-endorphin and the inhibition of cortisol, thus affecting the composition's efficacy in improving negative emotions and providing a calming and soothing effect. The composition obtained using *Edamame foetida* seed extract-2 showed the best effect in releasing β-endorphin and inhibiting cortisol (Example 18).

[0095] The β-endorphin increase rate in Comparative Examples 1-2 was 8-24%, and the cortisol decrease rate was 4-10%. This is because the mass ratio of each component in the compositions of Comparative Examples 1-2 was not within the scope of this invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0096] The β-endorphin increase rate of Comparative Examples 3-7 was 8-13%, and the cortisol decrease rate was 3-10%. This is because the absence of one of the components of the present invention in the compositions of Comparative Examples 3-7 affected the synergistic effect between the components and reduced the efficacy of the composition.

[0097] The β-endorphin increase rate of Comparative Examples 8-12 was 9-17%, and the cortisol decrease rate was 8-10%. This is because the compositions of Comparative Examples 8-12 replaced one of the components in the composition of the present invention with other plant extracts, which did not produce a synergistic effect with the specific components selected by the present invention, and also affected the synergistic effect between other components of the present invention, thus reducing the efficacy of the composition.

[0098] Application examples

[0099] The present invention provides an emulsion in application examples and comparative application examples, the components (mass percentage) of which are shown in Table 3; wherein, the mood-enhancing compositions used in application examples 1-21 are the mood-enhancing compositions prepared in examples 1-21, and the mood-enhancing compositions used in application examples 22-23 are the mood-enhancing compositions prepared in example 18; the mood-enhancing compositions used in comparative application examples 1-12 are the mood-enhancing compositions prepared in comparative examples 1-12; in addition, a blank application example is provided, the only difference between the blank application example and application example 1 is that no mood-enhancing composition is added.

[0100] Table 3:

[0101]

[0102]

[0103] This embodiment provides a method for preparing an emotional beauty lotion, the preparation method comprising the following steps:

[0104] (1) Mix the humectant, thickener and part of the pH adjuster (EDTA-disodium) with water and stir. Heat to 85°C and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared phase A.

[0105] (2) Mix the emulsifier, heat to 75°C, and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared phase B.

[0106] (3) Mix the preservatives and heat to 60°C to melt them to obtain the pre-prepared C phase;

[0107] (4) Heat the pre-prepared phase A to 80°C, add the pre-prepared phase B at 300 rpm, stir and mix, then cool down to 60°C, add the pre-prepared phase C at 300 rpm and stir and mix, then cool down to below 45°C, add the mood-enhancing composition and continue stirring for 8 minutes, finally add the remaining pH adjuster (arginine) to adjust the pH to 5.5-7.0, then stop stirring, discharge the material, and obtain the mood-enhancing emulsion.

[0108] Test Example 1: Human Skin Patch Test

[0109] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal volumes (0.020 mL-0.025 mL) of the test sample (emulsions prepared for the application example, control example, and blank example) were placed in a specific patch applicator. The patch was then applied to the volunteer's arm with hypoallergenic adhesive tape, gently pressed to ensure even application, and left on for 24 hours. The blank control group used distilled water, and the blank application examples used an emulsion without the emotional cosmetic composition. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 h, 24 h, and 48 h. The severity of adverse skin reactions is shown in Table 4 below.

[0110] Table 4

[0111]

[0112] After testing, the mood-enhancing compositions provided in Application Examples 1-23, Comparative Application Examples 1-12, and Blank Application Examples of the present invention all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0113] Test Example 2: Human Efficacy Test for Improving Mood, Soothing, and Controlling Oil Production

[0114] Experimental Methods: Following the "Cosmetic Safety Technical Specifications" (2015), 180 Asian adults aged 18-60 with oily, sensitive skin were selected and randomly divided into 36 groups of 5 participants each. Volunteers applied the sample (prepared from application examples 1-23, control examples 1-12, and a blank application example) to their entire face twice daily, morning and evening. Data was collected on day 0 and day 28. After the initial application, volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room at 21±1℃ and 50±10% humidity. Volunteers completed the Self-Assessment Manikin (SAM) scale to evaluate their current skin sensations, using a 9-point scale, with higher scores indicating greater pleasure. After 30 minutes of quiet sitting, facial photography was performed using a Visia-7 camera, and the resulting red area images were analyzed to obtain the a* value. After 2 hours of quiet sitting, forehead oil content data was collected using a Sebumeter SM 815 probe.

[0115] The ability to improve mood is represented by the improvement in the SAM scale score; a higher improvement rate indicates a stronger ability to improve mood. The ability to soothe is represented by the improvement in the a* value; a higher improvement rate in the a* value indicates a stronger ability to soothe. The ability to control oil is represented by the improvement in forehead oil content; a higher improvement rate in oil content indicates a stronger ability to control oil. The formula is as follows:

[0116] SAM scale score improvement rate = [(sample day 28 - sample day 0) / sample day 0 - (blank application day 28 - blank application day 0) / blank application day 0] * 100%;

[0117] a* value improvement rate = [(sample day 0 - sample day 28) / sample day 0 - (blank application day 0 - blank application day 28) / blank application day 0] * 100%;

[0118] The improvement rate of oil content is calculated as follows: [(Sample Day 0 - Sample Day 28) / Sample Day 0 - (Blank Application Day 0 - Blank Application Day 28) / Blank Application Day 0] * 100%. The results are shown in Table 4.

[0119] Table 4:

[0120]

[0121]

[0122] As shown in Table 4, compared with the control group, the application group showed an improvement rate of over 26% in SAM scale score, over 14% in a* score, and over 19% in oil content after 28 days. This demonstrates that the composition of the present invention, through specific components within a certain weight ratio range, can improve the composition's SAM scale score, a* score, and oil content improvement rate, thereby enhancing the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production.

[0123] As can be seen from Application Examples 1-7, the mass ratio of each component in the composition of the emulsion product affects the product's ability to improve mood, provide a pleasant feeling to the skin, relieve stress, soothe, and control oil. When the mass ratio of the components is limited to the sum of the masses of Cuban balsam resin and acetyl tetrapeptide-15, the sum of the masses of grey soybean seed extract and citrus fruit extract, and the mass ratio of catnip extract is (0.11-6):(0.11-7):(0.01-3), the product has a good effect on improving SAM scale scores, a* improvement rate, and oil content improvement rate, thereby enhancing the product's ability to improve mood, provide a pleasant feeling to the skin, relieve stress, soothe, and control oil. When the mass ratio is further selected as (0.55-3.5):(0.3-5.5):(0.1-1), the composition has even better effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil. The best effect is achieved when the mass ratio is 1.2:2.8:0.5 (Application Example 7).

[0124] This invention investigated the effect of the mass ratio of guacamole resin and acetyl tetrapeptide-15 on the improvement of SAM scale scores, a* improvement rate, and oil content improvement rate of the composition through Application Examples 8-10. Application Examples 8-10 show that the mass ratio of guacamole resin and acetyl tetrapeptide-15 affects the composition's effect on SAM scale scores, a* improvement rate, and oil content improvement rate, thereby influencing the composition's efficacy in improving mood, providing skin pleasure, relieving stress, soothing, and controlling oil production. When the mass ratio of guacamole resin and acetyl tetrapeptide-15 is limited to the range of (0.1-5):(0.01-1), the composition's efficacy in improving mood, providing skin pleasure, relieving stress, soothing, and controlling oil production is enhanced. Further optimization by limiting the mass ratio of guacamole resin and acetyl tetrapeptide-15 to the range of (0.5-3):(0.05-0.5) yields even better results. The effect was optimal when the mass ratio of Cuban guar gum resin to acetyl tetrapeptide-15 was 1:0.2 (Application Example 8).

[0125] This invention investigated the effect of the mass ratio of soybean seed extract to citrus fruit extract on the ability of the composition to improve SAM scale scores, a* improvement rate, and oil content improvement rate through application examples 11-13. Application examples 11-13 show that the mass ratio of soybean seed extract to citrus fruit extract affects the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production. When the mass ratio of soybean seed extract to citrus fruit extract is limited to the range of (0.1-5):(0.01-2), the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production are enhanced. Further optimization by limiting the mass ratio of soybean seed extract to citrus fruit extract to the range of (0.2-4):(0.1-1.5) yields even better results. The optimal effect is achieved when the mass ratio of soybean seed extract to citrus fruit extract is 1.8:1 (application example 12).

[0126] This invention investigated the effect of the mass ratio of Nepeta cataria extract to other components in component C on the improvement of SAM scale scores, a* improvement rate, and oil content improvement rate through application examples 14-16. Application examples 12-14 show that the mass ratio of Nepeta cataria extract to other components affects the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production. When the mass ratio of the combined mass of Cuban balsam resin and acetyl tetrapeptide-15, the combined mass of grey soybean seed extract and citrus fruit extract, and Nepeta cataria extract is in the range of (0.11-6):(0.11-7):(0.01-3), the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production are enhanced. Further optimization of the mass ratio of the combined mass of Cuban balsam resin and acetyl tetrapeptide-15, the combined mass of grey soybean seed extract and citrus fruit extract, and Nepeta cataria extract to the range of (0.55-3.5):(0.3-5.5):(0.1-1) yields an even better composition. When the mass ratio is further selected as 1.2:2.8:0.5 (Application Example 7), the effect is optimal.

[0127] In summary, the optimal ratio of the composition of the present invention is: Cuban balsamic resin: acetyl tetrapeptide-15: edamame seed extract: citrus fruit extract: catnip extract = 1:0.2:1.8:1:0.5 (Application Example 17).

[0128] This invention investigated the effects of different extraction methods on the improvement of SAM scale scores, a* improvement rate, and oil content improvement rate of the composition through Application Examples 18-21. Application Examples 18-21 show that the extraction method of the soybean seed extract has a certain impact on the improvement of SAM scale scores, a* improvement rate, and oil content improvement rate, thereby affecting the composition's effects on improving mood, providing a pleasant feeling to the skin, relieving stress, soothing, and controlling oil production. The composition obtained using soybean seed extract-2 showed the best effect (Application Example 18).

[0129] Compared with Application Examples 1-2, the improvement rate of SAM scale score was 3-5%, the improvement rate of a* was about 3%, and the improvement rate of oil content was 4-5%. This is because the mass ratio of each component in the composition of Application Examples 1-2 was not within the scope of the present invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0130] Compared with Application Examples 3-7, the improvement rate of SAM scale score was 2-6%, the improvement rate of a* was 2-3%, and the improvement rate of oil content was 2-5%. This is because the absence of one of the components of the present invention in the composition of Application Examples 3-7 affected the synergistic effect between the components and reduced the efficacy of the composition.

[0131] Compared with the SAM scale score improvement rate of 2-6%, the a* improvement rate of 2-4%, and the oil content improvement rate of 2-5% in the comparative application examples 8-12, this is because the composition of the comparative application examples 8-12 replaced one of the components in the composition of the present invention with other plant extracts, which did not produce a synergistic effect with the specific components selected in the present invention, and also affected the synergistic effect between other components of the present invention, thus reducing the efficacy of the composition.

[0132] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A mood-enhancing composition, characterized in that, The composition comprises the following components in parts by weight: 0.08-6 parts of Cuban gum resin, 0.005-2 parts of acetyl tetrapeptide-15, 0.06-6 parts of grey soybean seed extract, 0.008-3 parts of citrus fruit extract, and 0.008-4 parts of catnip extract; The preparation method of the edamame seed extract includes the following steps: After drying, the seeds of the grey soybean are crushed and added to an aqueous solution of a eutectic solvent for ultrasonic extraction. The product is collected, centrifuged, and the supernatant is taken to obtain the grey soybean seed extract. The eutectic solvent is composed of betaine and oxalic acid. The preparation method of the edamame seed extract satisfies at least one of the following: (a) The mass ratio of betaine to oxalic acid is betaine: oxalic acid = 1: (1~3); (b) The mass concentration of the eutectic solvent in the aqueous solution of the eutectic solvent is 20-60%; (c) The temperature of the ultrasonic extraction is 40~60℃; or / and the power of the ultrasonic extraction is 150~200W, and the time of the ultrasonic extraction is 20~50 min; (d) The material-to-liquid ratio for ultrasonic extraction is 1g:(20~40)mL.

2. The mood-enhancing composition as described in claim 1, characterized in that, The mass ratio of the following components in the composition is: (Cuba gum resin + Acetyl tetrapeptide-15): (Gray soybean seed extract + Citrus fruit extract): Nepeta extract = (0.11-6): (0.11-7): (0.01-3).

3. The mood-enhancing composition as described in claim 2, characterized in that, The mass ratio of the following components in the composition is: (Cuba gum resin + Acetyl tetrapeptide-15): (Gray soybean seed extract + Citrus fruit extract): Nepeta extract = (0.55-3.5): (0.3-5.5): (0.1-1).

4. The mood-enhancing composition as described in claim 1, characterized in that, In the composition, the mass ratio of Cuban guar gum resin to acetyl tetrapeptide-15 is Cuban guar gum resin:acetyl tetrapeptide-15 = (0.1-5):(0.01-1).

5. The mood-enhancing composition as described in claim 4, characterized in that, In the composition, the mass ratio of Cuban guar gum resin to acetyl tetrapeptide-15 is Cuban guar gum resin:acetyl tetrapeptide-15 = (0.5-3):(0.05-0.5).

6. The mood-enhancing composition as described in claim 1, characterized in that, In the composition, the mass ratio of soybean seed extract to citrus fruit extract is soybean seed extract:citrus fruit extract = (0.1-5):(0.01-2).

7. The mood-enhancing composition as described in claim 6, characterized in that, In the composition, the mass ratio of soybean seed extract to citrus fruit extract is soybean seed extract:citrus fruit extract = (0.2-4):(0.1-1.5).

8. The use of the mood-enhancing composition according to any one of claims 1-7 in the preparation of cosmetics.

9. An emulsion, characterized in that, The emulsion comprises the following components by weight percentage: 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH adjuster, 0.5-5% emulsifier, 0.5-3% preservative, 1-10% of the emotional beauty composition as described in any one of claims 1-7, and the balance being water.

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