A composition for relieving itch and improving barrier in sensitive skin and use thereof

Abstract

The application discloses a composition for relieving itching of sensitive skin and improving barrier and application, and relates to the technical field of cosmetics.The composition comprises component A and component B;the component A comprises flaxseed extract and roselle fruit extract; and the component B comprises annona glabra extract and burscckia yunnanensis extract.The flaxseed extract, the roselle fruit extract, the annona glabra extract and the burscckia yunnanensis extract are combined to find that the flaxseed extract, the roselle fruit extract, the annona glabra extract and the burscckia yunnanensis extract have a synergistic effect, and the composition formed by the specific combination has the effects of relieving facial itching of sensitive skin, reducing excessive activation of neurons and inhibiting inflammation, and repairing an objective barrier and moisturizing.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a composition for relieving itching of sensitive skin and improving barrier function and its application. Background Art

[0002] Sensitive skin refers to a type of skin that exhibits an unusually strong reaction to external stimuli, such as temperature changes, chemicals, friction, and pressure. It typically manifests as irritation-induced redness, itching, burning, or stinging. Redness, burning, and stinging are the most easily detected and frequently discussed symptoms, while facial itching receives less attention. This is because the triggers of itching are often subtler and harder to pinpoint than other symptoms, making it more difficult to predict and resolve. In many cases, the itch reaction can be more complex, involving physiological triggers as well as psychological and emotional factors. Itching typically persists for a long time, while redness, stinging, and burning typically disappear once the injury subsides. Prolonged itching can cause anxiety and restlessness, impacting mood and sleep quality. Furthermore, itching triggers the body's defensive responses, such as the release of chemicals like histamine, which further exacerbates the itching and leads to skin inflammation. People often scratch to relieve itching, but repeated scratching can lead to skin scaling and damage, exacerbating secondary irritation and creating a vicious cycle. So, this is what people usually say, "The pain can be tolerated, but the itch cannot."

[0003] The pruritus problem of sensitive skin is the result of the combined effects of multiple factors, including (1) impaired skin barrier function. Sensitive skin is usually accompanied by a weak epidermal stratum corneum, which makes the skin more susceptible to moisture loss, leading to dryness and itching; (2) increased neurosensitivity, including excessive activation of neurons and the release of neurotransmitters, such as the release of calcitonin gene-related peptide (CGRP), which enhances pain and itch perception, and can also trigger local inflammation and promote the release of pro-inflammatory factors; (3) inflammatory response, the activation of inflammatory cells and the release of cytokines can cause pruritus, such as mast cell degranulation and the release of pro-inflammatory cytokines; (4) external chemicals and certain skin care products can cause irritation and trigger itching; (5) stress and anxiety may further aggravate the pruritus by activating the skin's neural mechanisms. Therefore, improving the pruritus problem of sensitive skin requires barrier repair, replenishing skin moisture and lipids, reducing excessive neuronal activation and inflammatory response, and relieving stress and anxiety.

[0004] Currently, most products on the market prioritize analgesia and redness relief. There are few products specifically targeting itching of sensitive skin, and no product comprehensively improves itching from the above five aspects. Summary of the Invention

[0005] Based on this, the purpose of the present application is to overcome the shortcomings of the prior art and provide a composition for relieving sensitive skin itching and improving barrier and application.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present application is:

[0007] In the first aspect, the present application provides a composition for relieving sensitive skin itching and improving barrier, which comprises component A and component B; the component A comprises flaxseed extract and roselle fruit extract; the component B comprises annona glabra extract and b. capreous extract.

[0008] Flaxseed extract is rich in Omega-3 fatty acids, antioxidant substances and phytoestrogens, which can effectively moisturize and repair the skin. It has anti-inflammatory properties, can reduce skin irritation and improve inflammatory symptoms, and can also enhance the function of lamellar bodies, promote the tight junction of keratinocytes and barrier repair, and enhance the moisturizing ability of the skin. In addition, flaxseed extract also has antioxidant properties, can resist environmental stress, help slow down skin aging, and make the skin smoother and younger. At the same time, it helps to improve skin color and make the skin brighter and more uniform.

[0009] Roselle fruit extract is rich in vitamin C and polyphenols, and has significant skin care effects. Its strong antioxidant properties can effectively neutralize free radicals, slow down skin aging, and promote collagen synthesis to improve skin elasticity. In addition, roselle fruit extract has good moisturizing effect, which can improve the moisture content of the skin and make the skin more moisturizing and smooth. At the same time, its anti-inflammatory properties help to relieve skin irritation and restore the skin to a healthy state, showing a natural luster.

[0010] Annona glabra extract has a significant effect on improving skin itching, mainly by inhibiting calcitonin gene-related peptide (CGRP) and promoting the synthesis of beta-endorphin. CGRP plays an important role in triggering itching and inflammatory response, and the inhibitory effect of the extract can effectively reduce the itching and discomfort, and reduce the inflammatory response of the skin. On the other hand, beta-endorphin is known as a "natural analgesic", its promotion helps to improve the comfort of the skin, relieve the discomfort caused by itching, make the skin better repaired and balanced, and improve the overall health status.

[0011] B. capreous extract is rich in selenium polysaccharide, a complex polysaccharide compound of selenium. This ingredient has strong anti-inflammatory and antioxidant properties, which can relieve the itching caused by allergy or inflammation. Selenium polysaccharide can regulate the immune response of the skin and inhibit the release of inflammatory mediators, thereby reducing the symptoms of pruritus. At the same time, it can also moisturize and repair the damaged skin barrier, enhance the moisturizing ability of the skin, further reduce the discomfort of dryness and itching, and promote the healthy state and comfort of the skin.

[0012] The present invention has found that when the above-mentioned plant extracts are used in combination, they can synergistically improve facial itching in people with sensitive skin, reduce excessive neuronal activation and inhibit inflammation, as well as repair objective barriers and moisturize the skin. It has the effects of relieving itching, calming, soothing and smoothing the skin.

[0013] As a preferred embodiment of the first aspect, the mass ratio of component A to component B in the composition is component A:component B=(0.6-3):(0.02-1.5).

[0014] Illustratively, the mass ratio of component A to component B can be any point value between (0.6-3):(0.02-1.5) or a range value between any two points, such as 0.6:0.02, 0.6:0.05, 0.6:0.1, 0.6:0.5, 0.6:1, 0.6:1.5, 1:0.02, 1:1, 1:1.5, 2:0.02, 2:0.1, 2:0.5, 2:1, 2:1.5, 3:0.02, 3:0.1, 3:0.5, 3:1, 3:1.5.

[0015] As a preferred embodiment of the first aspect, the mass ratio of component A to component B in the composition is component A:component B=(1.2-2.3):(0.2-0.7).

[0016] As a preferred embodiment of the first aspect, the mass ratio of component A to component B in the composition is component A:component B=1.6:0.4.

[0017] Research conducted by the present invention has found that the mass ratio of component A to component B in a composition for alleviating pruritus and improving the barrier function of sensitive skin can, to a certain extent, affect its ability to inhibit neuronal overactivation and inflammation, thereby impacting the composition's efficacy in alleviating pruritus. This can also affect the composition's objective barrier-repairing and hydrating properties. Further selecting a mass ratio of component A to component B of (0.6-3):(0.02-1.5), and particularly (1.2-2.3):(0.2-0.7), yields even more superior antipruritic and anti-inflammatory effects. The composition also significantly enhances its inhibitory effects on calcitonin gene-related peptide (CGRP) and IL-33, and also improves its objective barrier-repairing and hydrating properties. Research has found that a mass ratio of component A to component B of 1.6:0.4 achieves the best overall efficacy.

[0018] As a preferred embodiment of the first aspect, in the component A, the mass ratio of linseed extract to roxburghii fruit extract is linseed extract:roxburghii fruit extract=(0.1-1):(0.5-2).

[0019] Illustratively, in component A, the mass ratio of flaxseed extract and roxburghii fruit extract can be any point value between 1:(0.1-1):(0.5-2) or a range value between any two points, such as 0.1:0.5, 0.1:1.5, 0.1:2, 0.5:1.5, 0.5:2, 1:0.5, 1:1, 1:2.

[0020] As a preferred embodiment of the first aspect, in the component A, the mass ratio of linseed extract to roxburghii fruit extract is linseed extract:roxburghii fruit extract = (0.4-0.8): (0.8-1.5).

[0021] As a preferred embodiment of the first aspect, in the component A, the mass ratio of linseed extract to roxburghii fruit extract is linseed extract:roxburghii fruit extract=0.5:1.1.

[0022] The present invention has found that the mass ratio of flaxseed extract and roxburghii fruit extract in component A of the composition for relieving itching of sensitive skin and improving barrier function will affect the composition's inhibitory effect on inflammatory factors and barrier repair efficacy to a certain extent, thereby affecting the composition's anti-inflammatory and barrier repair capabilities. When the mass ratio of flaxseed extract and roxburghii fruit extract is further selected to be (0.1-1): (0.5-2), especially (0.4-0.8): (0.8-1.5), the composition's anti-inflammatory and barrier repair capabilities can be enhanced. Studies have found that when the mass ratio of flaxseed extract and roxburghii fruit extract is 0.5:1.1, the composition of the present invention has the best anti-inflammatory and barrier repair effects.

[0023] As a preferred embodiment of the first aspect, in the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract is: Annona mume extract: Cyperus rotundus extract = (0.01-1): (0.01-0.5).

[0024] Illustratively, in the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract can be any point value between (0.01-1): (0.01-0.5) or a range value between any two points, for example, 0.01:0.01, 0.01:0.1, 0.01:0.5, 0.05:0.01, 0.05:0.2, 0.05:0.5, 1:0.01, 1:0.1, 1:0.2, or 1:0.5.

[0025] As a preferred embodiment of the first aspect, in the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract is:Annona mume extract:Cyperus rotundus extract=(0.1-0.5):(0.1-0.2).

[0026] As a preferred embodiment of the first aspect, in the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract is Annona mume extract:Cyperus rotundus extract=0.28:0.12.

[0027] Research has found that the mass ratio of the Annona mume extract to the Cyperus rotundus extract in component B of a composition for alleviating itching on sensitive skin and improving its barrier function can, to a certain extent, affect the composition's inhibitory effect on calcitonin gene-related peptide and moisturizing efficacy, thereby affecting the composition's ability to alleviate itching and moisturize. Further selecting a mass ratio of the Annona mume extract to the Cyperus rotundus extract of (0.01-1):(0.01-0.5), particularly (0.1-0.5):(0.1-0.2), can enhance the composition's ability to alleviate itching and moisturize. Research has found that a mass ratio of Annona mume extract to Cyperus rotundus extract of 0.28:0.12 achieves optimal itching-alleviating and moisturizing abilities.

[0028] As a preferred embodiment of the first aspect, the Cyperus rotundus extract is prepared by the following method:

[0029] The above-ground part of Cyperus rotundus is dried and then crushed, the powder is added into water, and extraction is performed by a subcritical water extraction method. The extracted product is collected and centrifuged, and the supernatant is taken to obtain the Cyperus rotundus extract; wherein the content of selenium polysaccharide in the Cyperus rotundus extract is 20-50%.

[0030] As a preferred embodiment of the first aspect, the preparation method of the Cyperus rotundus extract satisfies at least one of the following conditions:

[0031] (a) the solid-to-liquid ratio of the pennywort powder to water is 1:(10-40);

[0032] (b) The subcritical water extraction method uses a high-pressure reactor as a reaction vessel; wherein the pressure of the high-pressure reactor is 5-9 MPa, the extraction temperature is 100-150° C., and the extraction time is 15-30 minutes.

[0033] The selenium polysaccharide content in the Cyperus rotundus extract obtained by different preparation methods of the present invention is different. When the solid-liquid ratio in the preparation method is 1:10; the extraction time is 15 minutes; the extraction temperature is 100°C and the pressure is 8MPa, the selenium polysaccharide content in the Cyperus rotundus extract obtained is the highest, and the effect of the composition prepared thereby is better.

[0034] In a second aspect, the present invention provides use of the composition for alleviating itching of sensitive skin and improving barrier function as described in the first aspect in the preparation of cosmetics.

[0035] As a preferred embodiment of the second aspect, the cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

[0036] In a third aspect, the present application provides a cosmetic product comprising the composition for relieving the itch and improving the barrier of sensitive skin.

[0037] As a preferred embodiment of the third aspect, the cosmetic product comprises the following components by mass percentage: the composition for relieving the itch and improving the barrier of sensitive skin 0.5-5%, a cosmetic base 4-40%, and water in the balance.

[0038] As a preferred embodiment of the third aspect, the cosmetic base comprises at least one of a thickening agent, a humectant, an emulsifier, a preservative, and a pH adjuster.

[0039] As a preferred embodiment of the third aspect, the humectant comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, hydrolyzed sodium hyaluronate, 1,2-butanediol, glycerin, pullulan, D-panthenol, and ceramide.

[0040] As a preferred embodiment of the third aspect, the preservative comprises at least one of 1,2-propanediol, 1,2-hexanediol, 1,2-pentanediol, and p-hydroxyacetophenone.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] The present application discovers that the linseed extract, roxburgh rose fruit extract, annona glabra extract, and bisporeous bupleurum extract have a synergistic effect from a large number of plant extracts, and the composition formed by the specific combination has the effects of relieving the facial itch of sensitive skin, reducing the over-activation of neurons, inhibiting inflammation, repairing the objective barrier, and moisturizing. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 The facial desquamation of the volunteers in the application example 1 and the comparative application example 9 of the present application before and after use is compared in the following figure (wherein the more red indicates the more desquamation). DETAILED DESCRIPTION

[0044] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the examples and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials. Further description of the raw materials used in the present invention:

[0045] Flaxseed extract was purchased from Ashland under the trade name Lipigenine TM ; Rosa roxburghii fruit extract was purchased from Biocosmethic Company under the trade name VITACETHIC ® (Roxburgh fruit extract (and) maltodextrin); Annona cherimoya extract was purchased from the cosmetic raw material company CLR Berlin under the trade name AnnonaSense CLR TM ; Sweet almond seed extract was purchased from Xi'an Qiancao Biotechnology Co., Ltd.; wood apple extract was purchased from Guangzhou Luojie Biotechnology Co., Ltd., with the trade name of Tanaka Extract C-Ⅰ; Kava extract was purchased from Guangzhou Jianeng Biotechnology Co., Ltd.; Watercress extract was purchased from Gansu Yishengxiang Biotechnology Co., Ltd.

[0046] Preparation method of Cyperus rotundus extract 1:

[0047] The extraction was performed using the subcritical water extraction (SWE) method, and the specific steps were as follows:

[0048] (1) Collect the aerial parts of Cyperus rotundus, wash them, dry them at 45°C to constant weight, grind them, pass the powder through a 60-mesh sieve, collect them, and store them at room temperature;

[0049] (2) The powder was extracted in a high-pressure reactor at a pressure of 8 MPa at a solid-liquid ratio of 1:10 for 15 minutes at a temperature of 100°C to obtain a liquid extract;

[0050] (3) The liquid extract was centrifuged at 3000 rpm for 10 minutes, and the insoluble matter in the lower layer was removed to obtain the Cyperus rotundus extract. The content of selenium polysaccharide in the Cyperus rotundus extract was 50%.

[0051] Preparation method of Cyperus rotundus extract 2:

[0052] (1) Collect the aerial parts of Cyperus rotundus, wash them, dry them at 45°C to constant weight, grind them, pass the powder through a 60-mesh sieve, collect them, and store them at room temperature;

[0053] (2) in the high pressure reactor with 5MPa pressure, the powder is extracted with 1:30 solid-liquid ratio for 15 minutes, the extraction temperature is 150 DEG C, and the liquid extract is obtained;

[0054] (3) the liquid extract is centrifuged at 3000 rpm for 10 minutes, and the lower insoluble matter is removed, and the bupleurum falcatum extract is obtained.

[0055] The preparation method of the bupleurum falcatum extract 3 is as follows:

[0056] (1) the aboveground part of bupleurum falcatum is collected, washed, dried at 45 DEG C to constant weight, ground, the powder is passed through a 60 mesh sieve and collected, and dried at room temperature;

[0057] (2) in the high pressure reactor with 9MPa pressure, the powder is extracted with 1:40 solid-liquid ratio for 30 minutes, the extraction temperature is 120 DEG C, and the liquid extract is obtained;

[0058] (3) the liquid extract is centrifuged at 3000 rpm for 10 minutes, and the lower insoluble matter is removed, and the bupleurum falcatum extract is obtained.

[0059] The preparation method of the composition for relieving the itching of sensitive skin and improving the barrier comprises the following steps: the components are uniformly mixed according to the corresponding proportion to obtain the composition.

[0060] Examples and comparative examples

[0061] The application provides a composition for relieving the itching of sensitive skin and improving the barrier, and the components and weight parts of the composition are selected as shown in Table 1, wherein, the comparative examples take example 5 as a reference, and the total amount of the components lacking in comparative examples 3-6 is the same as that of example 5.

[0062] Table 1: components and weight parts of examples and comparative examples

[0063]

[0064] Effect example:

[0065] The composition is tested in terms of reducing the overactivation of neurons and inhibiting inflammation.

[0066] Experimental method: the effect of the composition prepared from the examples and the comparative examples on inhibiting the overactivation of neurons and inhibiting inflammation is explored.

[0067] Calcitonin gene-related peptide (CGRP) is a neurotransmitter that is massively transmitted when a stimulant contacts the skin, and the inflammatory factor IL-33 is also massively produced, which is a substance that mediates itching and scratching, causing a series of problems such as skin itching, redness, etc. During cell culture, the lower the content of CGRP, the lower the activation of neurons, the stronger the itching improvement effect of the composition, and the lower the content of the inflammatory factor IL-33, the stronger the anti-inflammatory effect of the composition.

[0068] The cell line used in the present application is human keratinocyte HaCaT (provided by Guangzhou Customs Inspection Center), and the test conditions are: incubator temperature 37±1℃, saturated humidity, carbon dioxide 5±1%; according to the grouping, the cells are cultured and treated, and then the content of calcitonin gene-related peptide (CGRP) and the content of inflammatory factor IL-33 are tested, and the test method is as follows:

[0069] (1) The cell suspension is inoculated in a culture bottle, and the culture density is 1×10 6 / mL, DMEM high-sugar culture solution (Gibco) containing 10% fetal bovine serum is used, and the culture is carried out for 24 hours;

[0070] (2) The control group and the sample group are treated with PBS buffer containing 100 μM histamine for 30 minutes, and then the PBS containing histamine is discarded and washed with PBS for 3 times;

[0071] (3) The control group is added with DMEM high-sugar culture solution containing 10% fetal bovine serum, and the sample group is added with 0.1% itching improvement composition prepared by the examples or the comparative examples (diluted with PBS) and 10% fetal bovine serum DMEM high-sugar culture solution, respectively, and the cells are collected after 24 hours of culture;

[0072] (4) CGRP content and IL-33 content determination: the activation level of neurons of the cells is detected by using calcitonin gene-related peptide (CGRP) ELISA kit, and the inflammatory factor content of the cells is detected by using inflammatory factor IL-33 ELISA kit.

[0073] Histamine can induce an increase in keratinocyte nerve sensory transmission, which is marked by an increase in calcitonin gene-related peptide (CGRP), so after sample treatment, the lower the content of calcitonin gene-related peptide (CGRP) and the lower the content of inflammatory factor IL-33, the lower the activity of cell neurons, and the stronger the antipruritic and anti-inflammatory ability of the composition. The antipruritic ability of the composition is represented by the decrease of the content of calcitonin gene-related peptide (CGRP), and the anti-inflammatory ability of the composition is represented by the decrease of the content of inflammatory factor IL-33. The calculation formula is as follows:

[0074] CGRP inhibition rate (%) = (1-CGRP 样品组 / CGRP对照组 )×100%;

[0075] Inflammation inhibition rate (%) = (1-IL-33 样品组 / IL-33 对照组 )×100%.

[0076] The results are shown in Table 2:

[0077] Table 2: Test results of the antipruritic and anti-inflammatory abilities of the compositions

[0078]

[0079] As can be seen from Table 2, the composition for alleviating itching of sensitive skin and improving barrier function provided by the present invention can inhibit itch nerve activation and inflammatory response, which indicates that the composition of the present invention has the efficacy of alleviating itching of sensitive skin and anti-inflammatory.

[0080] Compared with the comparative example group, the itch nerve activation inhibition rate and the inflammation inhibition rate of the embodiment group were higher than those of the comparative example group. It can be seen that the composition of the present invention can improve the itch nerve activation inhibition rate and the inflammation inhibition rate of the composition within a certain weight ratio range through specific components. The itch nerve activation inhibition rate is above 60%, and the inflammation inhibition rate is above 70%.

[0081] Examples 1-5 show that the mass ratio of the components in the composition for alleviating itching on sensitive skin and improving the barrier function affects the composition's ability to inhibit pruritic nerve activation and inflammatory responses. When the mass ratio of component A to component B is limited to a range of (0.6-3):(0.02-1.5), the resulting composition exhibits excellent pruritus and inflammation-reducing effects. Further, when the mass ratio of component A to component B is selected within the range of (1.2-2.3):(0.2-0.7), the resulting composition exhibits even better pruritic nerve activation and inflammatory response inhibition. The optimal effect is achieved when the mass ratio of component A to component B is 1.6:0.4 (Example 5).

[0082] The present invention studies the effect of the mass ratio of flaxseed extract to roxburghii fruit extract in component A on the ability of the composition to inhibit inflammation through Examples 6-8. From Examples 6-8, it can be seen that the mass ratio of flaxseed extract to roxburghii fruit extract affects the effect of the composition on inhibiting inflammation, thereby affecting the anti-inflammatory efficacy of the composition. When the mass ratio of flaxseed extract to roxburghii fruit extract is limited to the range of (0.1-1): (0.5-2), the anti-inflammatory efficacy of the composition can be improved. When the mass ratio of flaxseed extract to roxburghii fruit extract is further limited to the range of (0.4-0.8): (0.8-1.5), the effect of the resulting combination is even better. When the mass ratio of flaxseed extract to roxburghii fruit extract is 0.5:1.1 (Example 7), the effect is optimal.

[0083] The present invention investigated the effect of the mass ratio of Annona mume extract to Imperata cylindrica extract in component B on the pruritic nerve activation inhibitory ability of the composition through Examples 9-11. These results demonstrate that the mass ratio of Annona mume extract to Imperata cylindrica extract influences the composition's pruritic nerve activation inhibitory effect, thereby impacting the composition's anti-pruritic efficacy. A mass ratio of Annona mume extract to Imperata cylindrica extract within the range of (0.01-1):(0.01-0.5) enhanced the composition's anti-pruritic efficacy. Further, a mass ratio of Annona mume extract to Imperata cylindrica extract within the range of (0.1-0.5):(0.1-0.2) resulted in even greater efficacy. The optimal efficacy was achieved when the mass ratio of Annona mume extract to Imperata cylindrica extract was 0.28:0.12 (Example 10).

[0084] The results obtained in Examples 6-11 were combined to obtain the composition with the best effect in Example 12.

[0085] The present invention studies the effects of Cyperus rotundus extracts extracted by different methods on the efficacy of the composition. From Examples 12-14, it can be seen that the Cyperus rotundus extracts prepared by the methods of Examples 13-14 are less effective than that of Example 12.

[0086] The itch nerve activation inhibition rate of Comparative Example 1-2 is 40-50%, and the inflammation inhibition rate is about 60%. This is because the mass ratios of the components in the compositions of Comparative Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0087] The itch nerve activation inhibition rate of Comparative Example 3-6 is less than 30%, and the inflammation inhibition rate is about 30%. This is because the composition of Comparative Example 3-6 lacks one of the components of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0088] The itch nerve activation inhibition rate of Comparative Examples 7-10 is 30-40%, and the inflammation inhibition rate is 30-50%. This is because the compositions of Comparative Examples 7-10 replace one of the compositions of the present invention with other plant extracts, which does not produce a synergistic effect with the specific components selected by the present invention. It also affects the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0089] Application Examples

[0090] The application examples of the present invention and the comparative application examples provide a spray, the components (mass percentage) of the spray are shown in Table 3; wherein, the compositions for alleviating itching of sensitive skin and improving barrier function used in Application Examples 1-14 are the compositions for alleviating itching of sensitive skin and improving barrier function prepared in Examples 1-14, respectively; the compositions for alleviating itching of sensitive skin and improving barrier function used in Application Examples 15 and 16 are the compositions for alleviating itching of sensitive skin and improving barrier function prepared in Example 1; the compositions for alleviating itching of sensitive skin and improving barrier function used in Comparative Application Examples 1-10 are the compositions for alleviating itching of sensitive skin and improving barrier function prepared in Comparative Examples 1-10, respectively; and a blank application example is also provided, the only difference between the blank application example and Application Example 1 being that the composition for alleviating itching of sensitive skin and improving barrier function is not added;

[0091] Table 3:

[0092]

[0093] Application Example 1 provides the above-mentioned spray preparation method: after mixing all the ingredients, the mixture is placed in an aluminum aerosol can to form a spray. The spray preparation methods provided in Application Examples 2-16, Comparative Application Examples 1-10 and Blank Application Examples are consistent with those in Application Example 1.

[0094] Test application example: The spray has anti-itching, repairing, hydrating and moisturizing effects on sensitive skin. The specific method is as follows:

[0095] 1. Objective evaluation of barrier repair and hydration efficacy:

[0096] A total of 125 participants (20-50 years old) with sensitive skin (lactate-positive) and impaired skin barrier (TEWL ≥ 20 g / hm²) were recruited. A self-assessment questionnaire confirmed frequent itching (at least five times per month). Participants were asked to refrain from applying any products to the test area (face) on the day of their visit. After washing their face, they sat in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. They were then randomly divided into 25 groups, each consisting of five participants. Initial TEWL and initial moisture content were measured on the left and right sides of each participant's face using a TewaMeter® Hex (Courage + Khazaka) transepidermal water loss probe and a Corneometer® CM 825 skin moisture probe. The skin smoothness index (SEsm) was measured using a VC20, and the average value was calculated. Participants in each group used the spray morning and evening, and also used it whenever they felt itchy or uncomfortable. They applied the spray at least twice daily, with no limit on frequency. The spray was applied in three circular motions around the face to ensure complete moisturization. The first group of subjects used a blank application example (a blank spray without the composition), and the second group used application example 1. The TEWL value and moisture content of the facial skin after use were measured again on the 14th day and the 28th day, and the average values ​​were taken. The respective improvement rates (%) were then calculated using the corresponding average values ​​according to the following formula:

[0097] TEWL value improvement rate (%) = (initial TEWL value - TEWL value after use) / initial TEWL value × 100%;

[0098] Moisture content improvement rate (%) = (moisture content after use - initial moisture content) / initial moisture content × 100%;

[0099] SEsm value improvement rate (%) = (initial SEsm value - SEsm value after use) / initial SEsm value × 100%;

[0100] Among them, the greater the improvement rate of TEWL value, the stronger the spray's ability to repair skin barrier damage; the greater the improvement rate of skin moisture content, the stronger the spray's ability to moisturize and hydrate; the greater the improvement rate of SEsm value, the lower the skin roughness and the smoother the skin. The results are shown in Table 4:

[0101] Table 4: Objective evaluation results of barrier repair and hydration efficacy of various spray products

[0102]

[0103] Note: The blank application example does not contain the composition of the present invention, and the rest is the same as the application example.

[0104] As shown in Table 4, compared to the comparative example group, the TEWL value of the application example group improved by over 15%, the water content improved by over 20%, and the smoothness improved by over 30% after 28 days. This indicates that the composition of the present invention, through the use of specific components within a certain weight ratio range, can improve the composition's TEWL value improvement rate, water content improvement rate, and smoothness improvement rate, thereby improving the composition's ability to repair skin barrier damage, moisturize and hydrate the skin, smooth the skin, and improve roughness.

[0105] Application Examples 1-5 demonstrate that the mass ratio of components in a spray product affects the composition's TEWL value, water content, and smoothness. When the mass ratio of components A to B is limited to a range of (0.6-3):(0.02-1.5), the resulting product exhibits significant improvements in TEWL value, water content, and smoothness, thereby enhancing the product's ability to repair skin barrier damage, improve roughness, and moisturize. Further selecting a mass ratio of components A to B within the range of (1.2-2.3):(0.2-0.7) further enhances the composition's skin barrier damage repair, roughness improvement, and moisturizing properties. The optimal effect is achieved when the mass ratio of components A to B is 1.6:0.4 (Application Example 5).

[0106] The present invention studied the effect of the mass ratio of flaxseed extract to roxburghii fruit extract in component A on the product's ability to repair skin barrier damage through application examples 6-8. Application examples 6-8 show that the mass ratio of flaxseed extract to roxburghii fruit extract affects the composition's effect on improving TEWL values, thereby affecting the composition's efficacy in repairing skin barrier damage. When the mass ratio of flaxseed extract to roxburghii fruit extract is limited to the range of (0.1-1):(0.5-2), the composition's efficacy in repairing skin barrier damage can be improved. When the mass ratio of flaxseed extract to roxburghii fruit extract is further limited to the range of (0.4-0.8):(0.8-1.5), the resulting combination has an even better effect. When the mass ratio of flaxseed extract to roxburghii fruit extract is 0.5:1.1 (application example 7), the effect is optimal.

[0107] The present invention investigated the effect of the mass ratio of Annona mume extract to Cyperus rotundus extract in component B on the product's moisturizing, hydrating, and roughness-reducing abilities through application examples 9-11. These examples demonstrate that the mass ratio of Annona mume extract to Cyperus rotundus extract influences the composition's ability to improve skin moisture, thereby impacting the composition's moisturizing, hydrating, and roughness-reducing properties. Limiting the mass ratio of Annona mume extract to Cyperus rotundus extract to within the range of (0.01-1):(0.01-0.5) enhanced the composition's moisturizing, hydrating, and roughness-reducing properties. Further limiting the mass ratio of Annona mume extract to Cyperus rotundus extract to within the range of (0.1-0.5):(0.1-0.2) yielded even better results. The optimal effect was achieved when the mass ratio of Annona mume extract to Cyperus rotundus extract was 0.28:0.12 (application example 10).

[0108] The present invention studies the effects of Cyperus rotundus extracts extracted by different methods on the efficacy of the composition. It can be seen from Application Examples 12-14 that the Cyperus rotundus extracts prepared by the methods of Application Examples 13-14 are less effective than that of Application Example 12.

[0109] The TEWL value improvement rate of comparative application examples 1-2 is 10-11%, the water content improvement rate is 17-18%, and the smoothness improvement rate is less than 30%. This is because the mass ratios of the components in the compositions of comparative examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0110] The TEWL value improvement rate of comparative application examples 3-6 is 6-7%, the water content improvement rate is 14-15%, and the smoothness improvement rate is less than 20%. This is because the compositions of comparative examples 3-6 lack one of the components of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0111] The TEWL value improvement rate of comparative application examples 7-10 is about 6%, the water content improvement rate is 12-14%, and the smoothness improvement rate is lower than 16%. This is because the compositions of comparative application examples 7-10 replace one of the components in the combination group of the present invention with other plant extracts, which do not produce a synergistic effect with the specific components selected by the present invention, and also affect the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0112] 2. Subjective evaluation of pruritus improvement:

[0113] Since itching is a subjective feeling that cannot be quantified at present, the effect of the spray on relieving itching on the face of sensitive skin people is calculated by using the nimodipine method, the initial skin symptoms of the subjects are observed, including skin redness, skin rash, dryness, tingling, burning, itching and other discomfort, and the conditions are recorded. Among them, slight scaling, slight dryness, slight itching discomfort are recorded as 1 point, the above-mentioned feelings are obviously recorded as 2 points, and severe is recorded as 3 points. Improvement index = (pre-treatment score - post-treatment score) / pre-treatment score x 100%, according to the curative effect index, it is divided into four grades of complete improvement, obvious improvement, improvement and invalid. Complete improvement (improvement index > 90%), obvious improvement (improvement index 60%-89%), improvement (improvement index 20%-59%), and invalid (improvement index < 20%).

[0114] Total effective rate % = (number of complete improvement cases + number of obvious improvement cases + number of improvement cases) / total number of treatments x 100%.

[0115] Table 5: Subjective itching improvement evaluation results

[0116]

[0117] Note: The blank application example does not add the composition of the present application, and the rest is the same as the application example.

[0118] As can be seen from Table 5, compared with the comparative example group, the total effective rate of itching improvement of the application example group is more than 75%. It can be seen that the composition of the present application can improve the itching improvement ability of the product by specific components within a certain weight ratio range.

[0119] As can be seen in application examples 1-5, the mass ratio of components in the spray product will affect the itching improvement ability of the composition. When the mass parts of the components are limited to the mass ratio of component A and component B (0.6-3):(0.02-1.5), the itching improvement ability of the product is improved, and when the mass ratio of component A and component B is further selected in the range of (1.2-2.3):(0.2-0.7), the itching improvement effect of the obtained composition is better, and when the mass ratio of component A and component B is 1.6:0.4, the effect is best.

[0120] The present invention studies the mass ratio of linseed extract and roxburghii fruit extract in component A through application examples 6-8 to determine whether the mass ratio of linseed extract and roxburghii fruit extract affects the ability of the product to improve pruritus. It can be seen from application examples 6-8 that the mass ratio of linseed extract and roxburghii fruit extract affects the effect of the composition on improving pruritus. This is because the mass ratio of linseed extract and roxburghii fruit extract affects the anti-inflammatory efficacy of the combination, thereby affecting the overall efficacy of the composition in improving pruritus. When the mass ratio of linseed extract and roxburghii fruit extract is limited to the range of (0.1-1): (0.5-2), the efficacy of the composition in improving pruritus can be improved. When the mass ratio of linseed extract and roxburghii fruit extract is further limited to the range of (0.4-0.8): (0.8-1.5), the effect of the resulting composition is better. When the mass ratio of linseed extract and roxburghii fruit extract is 0.5:1.1, the effect is best.

[0121] The present invention investigated the effect of the mass ratio of Annona mume extract to Cyperus rotundus extract in component B on the product's ability to alleviate pruritus through Application Examples 9-11. These results demonstrate that the mass ratio of Annona mume extract to Cyperus rotundus extract influences the composition's pruritus-relieving effect. Limiting the mass ratio of Annona mume extract to Cyperus rotundus extract to within the range of (0.01-1):(0.01-0.5) enhances the composition's pruritus-relieving efficacy. Further limiting the mass ratio of Annona mume extract to Cyperus rotundus extract to within the range of (0.1-0.5):(0.1-0.2) yields even greater efficacy. The optimal effect is achieved when the mass ratio of Annona mume extract to Cyperus rotundus extract is 0.28:0.12.

[0122] The present invention studies the effects of Cyperus rotundus extracts extracted by different methods on the efficacy of the composition. It can be seen from Application Examples 12-14 that the Cyperus rotundus extracts prepared by the methods of Application Examples 13-14 are less effective than that of Application Example 12.

[0123] The total effective rate of itching improvement in Comparative Application Example 1-2 is 60%. This is because the mass ratios of the components in the compositions of Comparative Application Example 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0124] The total effective rate of itching improvement in comparative application examples 3-6 is 20-60%. This is because the lack of one of the components of the present invention in the composition of comparative application examples 3-6 affects the synergistic effect between the components and reduces the efficacy of the composition.

[0125] The total effective rate of itching improvement in Comparative Application Examples 7-10 is 20%. This is because the composition of Comparative Application Examples 7-10 replaces one of the components in the combination group of the present invention with other plant extracts, which does not produce a synergistic effect with the specific components selected by the present invention, and also affects the synergistic effect between other components of the present invention, thereby reducing the efficacy of the composition.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for relieving itching of sensitive skin and improving barrier function, characterized in that: The composition comprises component A and component B; The component A comprises flaxseed extract and roxburghii fruit extract; The component B comprises an extract of Annona mume and an extract of Cyperus rotundus; The mass ratio of component A to component B in the composition is component A: component B = (0.6-3): (0.02-1.5); In the component A, the mass ratio of flaxseed extract and roxburghii fruit extract is flaxseed extract:roxburghii fruit extract=(0.1-1):(0.5-2); In the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract is: Annona mume extract: Cyperus rotundus extract = (0.01-1): (0.01-0.5).

2. The composition according to claim 1, wherein The mass ratio of component A to component B in the composition is component A:component B=(1.2-2.3):(0.2-0.7).

3. The composition according to claim 1, wherein In the component A, the mass ratio of flaxseed extract to roxburghii fruit extract is flaxseed extract:roxburghii fruit extract=(0.4-0.8):(0.8-1.5).

4. The composition according to claim 1, wherein In the component B, the mass ratio of the Annona mume extract to the Cyperus rotundus extract is: Annona mume extract: Cyperus rotundus extract = (0.1-0.5): (0.1-0.2).

5. The composition according to any one of claims 1 to 4, wherein The Cyperus rotundus extract is prepared by the following method: The above-ground part of Cyperus rotundus is dried and then crushed, the powder is added into water, and extraction is performed by subcritical water extraction method, the extracted product is collected and centrifuged, and the supernatant is taken to obtain the Cyperus rotundus extract; wherein the content of selenium polysaccharide in the Cyperus rotundus extract is 20-50%.

6. The composition according to claim 5, wherein At least one of the following is met: (a) the solid-to-liquid ratio of the pennywort powder to water is 1:(10-40); (b) A high-pressure reactor is used as a reaction vessel in the subcritical water extraction method; wherein the pressure of the high-pressure reactor is 5-9 MPa, the extraction temperature is 100-150° C., and the extraction time is 15-30 minutes.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. The use according to claim 7, characterized in that The cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

9. A cosmetic, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 6.

10. The cosmetic according to claim 9, wherein The cosmetic comprises the following components in percentage by mass: 0.5-5% of the composition according to any one of claims 1 to 6, 4-40% of a cosmetic base, and the balance being water.

11. The cosmetic according to claim 10, wherein The cosmetic base includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, and a pH adjuster.

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