An oil control and moisturizing composition for oily sensitive skin and application thereof

Abstract

The present application relates to a kind of oil-sensitivity skin's oil control and moisturizing composition and application, belong to cosmetic technical field.The composition is composed of lactobacillus / fermented product extract of black mustard seed, Norway spruce leaf extract, palm-like kelp extract, flaxseed extract, the reasonable compounding of four ingredients, respectively from microecological regulation, source oil control, deep moisturizing and barrier repair etc.Synergistically play the effect of oil control and moisturizing.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to an oil-controlling and moisturizing composition for oily and sensitive skin and its application. Background Technology

[0002] Oily sensitive skin is a complex skin type characterized by both excessive sebum secretion and a weakened skin barrier. It manifests as "oily on the outside, dry on the inside," with high sebum production on the skin surface and low moisture content in the stratum corneum. This phenomenon is primarily due to excessive sebum secretion from the sebaceous glands, leading to clogged pores and an oily feel. Simultaneously, impaired skin barrier function results in increased transepidermal water loss and insufficient hydration of the stratum corneum. Furthermore, oily sensitive skin is often accompanied by a gut microbiota imbalance, with excessive proliferation of Propionibacterium acnes and a significant decrease in the abundance of aerobic bacteria such as Rhodococcus and Pseudomonas.

[0003] Currently, there are certain limitations to the care of oily and sensitive skin. First, it is difficult to achieve both oil control and moisturizing simultaneously. Traditional oil-control products often use physical adsorption or astringents to achieve immediate oil control effects. These methods may excessively adsorb lipids on the skin surface, further damaging the skin barrier. Second, a single mechanism of action is insufficient to comprehensively improve the complex skin problems of oily and sensitive skin. The causes of oily and sensitive skin involve multiple levels, including microecological imbalance, overactive sebaceous glands, barrier dysfunction, and inflammatory responses. Currently, most commercially available products focus on a single efficacy pathway and have failed to form a synergistic intervention plan with multiple targets and pathways. Third, there is insufficient attention to microecological regulation. Studies have shown that skin microecological imbalance (such as excessive proliferation of Propionibacterium acnes and reduced beneficial bacteria) is closely related to the occurrence and development of oily and sensitive skin. However, existing oil-control products rarely involve regulation at the microecological level.

[0004] Therefore, developing a multi-target composition that can simultaneously achieve microecological regulation, source oil control, deep moisturizing and barrier repair, and is gently adapted to the characteristics of oily and sensitive skin, has become an urgent technical problem to be solved in this field. Summary of the Invention

[0005] The purpose of this invention is to provide an oil-controlling and moisturizing composition and its application for oily and sensitive skin. The composition consists of Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate seaweed extract, and flaxseed extract. The rational combination of the four ingredients synergistically exerts oil-controlling and moisturizing effects from the aspects of microecological regulation, source oil control, deep moisturizing and barrier repair.

[0006] The objective of this invention can be achieved through the following technical solutions: In a first aspect, the present invention provides an oil-controlling and moisturizing composition for oily and sensitive skin. The raw materials of the oil-controlling and moisturizing composition include Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract. The mass ratio of Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract is (0.01-1):(0.1-2):(0.2-3):(0.01-2).

[0007] Lactobacillus / black mustard seed fermentation product extract can secrete antimicrobial peptides that directly disrupt the cell membrane of Propionibacterium acnes. The organic acids in the extract can also competitively inhibit the proliferation of Propionibacterium acnes. This component can selectively inhibit Propionibacterium spp., and the Lactobacillus fermentation product can also promote the proliferation of beneficial bacteria such as Staphylococcus epidermidis. Furthermore, this component can activate lipid synthesis pathways, promote ceramide production, and strengthen the stratum corneum structure.

[0008] Norway spruce leaf extract is rich in polysaccharides, flavonoids, polyphenols, and other components. It can inhibit the secretion of lipids by sebaceous gland cells, and triterpenoids can improve the skin's water-oil balance, reducing dryness and itching. In addition, inhibiting sebum secretion may reduce inflammation to some extent. Furthermore, the extract also contains anti-inflammatory components such as flavonoids, thus it also has a certain anti-inflammatory effect on the skin.

[0009] Kelp extract can promote keratinocyte differentiation, accelerate epidermal lipid synthesis, and strengthen the skin barrier function; its polysaccharide components have strong water absorption and film-forming properties, which can form a breathable protective layer on the skin surface and reduce moisture loss.

[0010] Alpha-linolenic acid in flaxseed extract can activate the PPARs signaling pathway, promote the production of endogenous ceramides and natural moisturizing factors in the skin, and enhance and repair the skin barrier function.

[0011] Furthermore, the mass ratio of the Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract is (0.1-0.5):(0.5-1.5):(0.5-2):(0.1-1).

[0012] Secondly, the present invention provides a method for preparing the oil-controlling and moisturizing composition for oily and sensitive skin as described in the first aspect, the method comprising the following steps: The Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract were mixed evenly to obtain the oil-controlling and moisturizing composition.

[0013] Thirdly, the present invention provides the application of the oil-controlling and moisturizing composition for oily and sensitive skin described in the first aspect in the preparation of cosmetics, wherein the cosmetics include toners, lotions, creams, masks, serums, and sprays.

[0014] Furthermore, the amount of the oil-controlling and moisturizing composition for oily and sensitive skin added to the cosmetic is 0.5wt%-8wt%.

[0015] Fourthly, the present invention provides a lotion containing the oil-controlling and moisturizing composition for oily and sensitive skin described in the first aspect.

[0016] Furthermore, the emulsion also contains excipients acceptable in the cosmetics field.

[0017] Furthermore, the excipients acceptable in the cosmetic field include at least one of solvents, humectants, pH adjusters, emollients, emulsifiers, thickeners, solubilizers, antioxidants, chelating agents, and fragrances.

[0018] The beneficial effects of this invention are: This invention provides an oil-controlling and moisturizing composition for oily and sensitive skin. The composition comprises Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract. The rational combination of these four ingredients synergistically exerts oil-controlling and moisturizing effects through microecological regulation, source oil control, deep moisturizing, and barrier repair. Specifically, the Lactobacillus / black mustard seed fermentation product extract selectively inhibits harmful bacteria such as Propionibacterium acnes by secreting antimicrobial peptides and organic acids, while simultaneously promoting the proliferation of beneficial bacteria such as Staphylococcus epidermidis, thus regulating the skin's microecological balance. Norway spruce leaf extract inhibits lipid secretion from sebaceous gland cells at the source, reducing excessive oil production. Palmate kelp extract forms a breathable moisturizing film on the skin surface and promotes keratinocyte differentiation. Flaxseed extract promotes the production of endogenous ceramides and natural moisturizing factors by activating the PPARs signaling pathway. This invention differs from traditional methods that use physical adsorption to control oil. While inhibiting excessive sebum secretion, it fundamentally solves the contradiction of water-oil imbalance in oily and sensitive skin by promoting the construction of the skin's own moisturizing system. Furthermore, the composition described in this invention can reduce the inflammatory response that may be caused by excessive sebum secretion, making it more suitable for people with oily and sensitive skin. Detailed Implementation

[0019] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0020] Lactobacillus / black mustard seed fermentation product extract was purchased from Provital under the product name 72130-HYDRAFENCE™ GNSV; The Norway spruce leaf extract was purchased from Norture, Canada, under the product name NorLight B01 H. The palmate kelp extract was purchased from Guangzhou Jusheng Chemical Co., Ltd., and its product name is palmate kelp extract. Flaxseed extract was purchased from Xi'an Heguan Biotechnology Co., Ltd., and its product name is Flaxseed Extract. Unless otherwise specified, all other materials and reagents used in all embodiments and comparative examples of this invention are commercially available.

[0021] The oil-controlling and moisturizing compositions of Examples 1-5 were prepared according to the formulas shown in Table 1. The preparation method of the oil-controlling and moisturizing compositions is to weigh the corresponding formula amounts of the components and mix them evenly to obtain the oil-controlling and moisturizing compositions.

[0022] Table 1. Components and dosage of the oil-controlling and moisturizing composition

[0023] Note: "-" indicates no addition; the total weight of the compositions prepared in Examples 1-5, Comparative Examples 1-6 and the following comparative examples is the same.

[0024] Comparative Example 7 Compared with Example 1, the difference is that Comparative Example 7 uses an equal weight of Bifida ferment filtrate instead of Lactobacillus / black mustard seed ferment extract, while the other components and their weight ratios remain the same as in Example 1.

[0025] Comparative Example 8 Compared with Example 1, the difference is that Comparative Example 8 uses an equal weight of meadowsweet extract instead of Norway spruce leaf extract, while the other components and their weight ratios remain the same as in Example 1.

[0026] Comparative Example 9 Compared with Example 1, the difference is that Comparative Example 9 uses an equal weight of Centella asiatica extract instead of Laminaria japonica extract, while the other components and their weight ratios remain the same as in Example 1.

[0027] Comparative Example 10 Compared with Example 1, the difference is that Comparative Example 10 uses oat extract instead of flaxseed extract in equal weight, while the other components and their weight ratios are the same as in Example 1.

[0028] Test Example 1: Experiment on the Inhibition of Lipid Synthesis by the Composition (1) Solution preparation: Test sample preparation: Take 0.10 mL of the composition sample of the example and the comparative example respectively, add 0.90 mL of sodium pyruvate-free DMEM (Gibco, C11965500BT) culture medium to prepare a 10% stock solution; Preparation of hydrocortisone solution: Weigh 0.01 g of hydrocortisone (Shanghai Yuanye Biotechnology, S31360) and add 1 mL of methanol to prepare a 10 mg / mL stock solution. Preparation of isotretinoin solution: Weigh 0.01 g of isotretinoin (Shanghai Dibai Biotechnology, H573005) and add 1 mL of methanol to prepare a 10 mg / mL stock solution; Culture medium: Take 10 mL of fetal bovine serum FBS (Macklin, F917980) and add 90 mL of sodium pyruvate-free DMEM culture medium to prepare sodium pyruvate-free DMEM culture medium containing 10% FBS. Maintenance medium: Take 1 mL of fetal bovine serum (FBS) and add 99 mL of sodium pyruvate-free DMEM culture medium to prepare sodium pyruvate-free DMEM culture medium containing 1% FBS. PBS buffer: purchased from Gibco, catalog number C10010500BT.

[0029] (2) Experimental Groups: Negative control group (NT): Maintenance culture medium; Model control group (M): maintenance culture medium + 10 ng / mL hydrocortisone; Positive control group (PC): 0.01 mM isotretinoin + maintenance culture medium + 10 ng / mL hydrocortisone; Test sample group (TA): 0.001% sample + maintenance culture medium + 10 ng / mL hydrocortisone; (3) Quantitative fluorescence assay for neutral lipids: The cell line used was human sebaceous gland cells SZ95 (Shanghai Qingqi Biotechnology, BFN60807569), passaged 7 times. The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. The cell suspension was seeded into 96-well cell culture plates (black bottom transparent type) at a density of 1.0 × 10⁶ cells per well. 4 Add 100 μL of culture medium to each well and incubate for 18-24 h to allow the cells to adhere to the well. Discard the supernatant and add 100 μL of the required components for the negative control group (NT), positive control group (PC), model control group (M), and test sample group (TA) according to the above experimental groups. Set up 3 replicates for each well, as shown in Table 2 below. Table 2

[0030] After incubation for 48 h, the supernatant was discarded, and the samples were washed twice with PBS. As shown in Table 2 above, 100 μL of 10 μg / mL Nile Red dye (Shanghai Yuanye Biotechnology, S02N11G129713) was added to the quantitative fluorescence experimental group, and 15 μg / mL of fluorescein diacetate (FDA) (Shanghai Yuanye Biotechnology, J22A11H122040) was added to the quantitative fluorescence control group. After incubation for 5 min, the released fluorescence was detected on a multifunctional microplate reader. Excitation wavelengths of 485 nm and 494 nm and absorption wavelengths of 565 nm and 523 nm were used to detect the fluorescence intensity of Nile Red and FDA, respectively. The result was determined using the ratio of Nile Red to FDA (OD ratio), calculated as follows: Neutral lipid percentage (%) = OD value of quantitative fluorescence experimental group / OD value of quantitative fluorescence control group × 100%; Relative neutral lipid content (%) = percentage of neutral lipids in the test sample group (or negative control group, positive control group) / percentage of neutral lipids in the model control group × 100%; The lower the relative neutral lipid content, the better the effect of inhibiting sebum secretion. The results are recorded in Table 3 below.

[0031] Table 3 Negative control group (NT) 57.5 Model control group (M) 100.0 Positive control group (PC) 70.9 Example 1 60.2 Example 2 63.1 Example 3 62.6 Example 4 69.8 Example 5 68.3 Comparative Example 1 87.4 Comparative Example 2 89.5 Comparative Example 3 88.2 Comparative Example 4 86.7 Comparative Example 5 82.6 Comparative Example 6 80.1 Comparative Example 7 83.7 Comparative Example 8 85.4 Comparative Example 9 84.3 Comparative Example 10 83.2 As shown in Table 3, the oil-controlling and moisturizing composition prepared by the present invention can effectively inhibit sebum secretion and has a good oil-controlling effect.

[0032] Test Example 2: Determination of the effect of the composition on the content of AQP-3 and FLG proteins The compositions used in the examples and comparative examples were used as test samples to determine their effects on AQP-3 and FLG protein content, and to evaluate their moisturizing and repairing functions. The methods are as follows: (1) Cell seeding: at a density of 2×10 5 Inoculate cells at 1 / mL into 6-well plates, add 2 mL of cell suspension to each well, and incubate overnight in an incubator (37°C, 5% CO2).

[0033] (2) When the observed cell density reaches 40%-60%, the composition of the example and the comparative example is diluted to 5 mg / mL with cell culture medium, and 2 mL is administered per well. At the same time, an equal amount of cell culture medium is added as a blank control group, and the mixture is cultured overnight; 3 replicates are set for each well.

[0034] (3) Sample processing: When the cell density reaches 70%-80%, discard the culture medium, wash with sterile PBS solution, discard the PBS after washing, add 0.5 mL of 0.25% trypsin-EDTA to each well for digestion, wash and resuspend the cells after digestion, sonicate the cells, centrifuge at 10000 rpm for 8 min, and collect the supernatant.

[0035] (4) ELISA detection: Perform the detection according to the instructions of the AQP-3 and FLG ELISA detection kit, and calculate the increase rate of AQP-3 and FLG content according to the following formula: AQP-3 content increase rate (%) = (AQP-3 content in sample group - AQP-3 content in blank control group) / AQP-3 content in blank control group × 100%; FLG content increase rate (%) = (FLG content in sample group - FLG content in blank control group) / FLG content in blank control group × 100%; The results are recorded in Table 4 below.

[0036] Table 4 Example 1 65.1 79.8 Example 2 60.8 75.3 Example 3 62.2 76.7 Example 4 57.5 72.6 Example 5 58.9 73.2 Comparative Example 1 42.7 50.9 Comparative Example 2 41.6 53.5 Comparative Example 3 40.3 54.1 Comparative Example 4 43.2 52.4 Comparative Example 5 47.8 59.8 Comparative Example 6 48.9 58.3 Comparative Example 7 47.1 55.2 Comparative Example 8 45.5 56.9 Comparative Example 9 44.3 57.6 Comparative Example 10 46.4 55.8 As shown in Table 4, the oil-controlling and moisturizing composition prepared by the present invention can effectively promote the increase of AQP-3 and FLG content, and has significant moisturizing, repairing and strengthening effects on the skin barrier.

[0037] Application Example 1-5, Comparative Application Example 1-10 The oil-controlling and moisturizing compositions for oily and sensitive skin of Examples 1-5 and Comparative Examples 1-10 were added to the lotions at a concentration of 3 wt% to obtain the lotions of Application Examples 1-5 and Comparative Application Examples 1-10, respectively. The formulations are shown in Table 5.

[0038] Table 5

[0039] The emulsion preparation methods described in Application Examples 1-5 and Comparative Application Examples 1-10 include the following steps: S1. Mix all components of phase A and heat to 80°C. Homogenize at 1000 rpm until evenly dispersed. After homogenization, keep warm for later use to obtain pre-prepared component A. S2. After mixing phase B, heat to 80°C and homogenize at 1000 rpm for 5 minutes. After homogenization, keep warm for later use to obtain pre-prepared component B. S3. Mix the C phase and heat and stir at 60°C until dissolved to obtain pre-prepared C component; mix the D phase components and stir until dissolved to obtain pre-prepared D component; S4. Heat the pre-formulated component A obtained in S1 to 85°C, add the pre-formulated component B at 35 rpm, mix evenly, then cool down to 60°C, add the pre-formulated component C and stir evenly, continue to cool down to 45°C, add the pre-formulated component D and the component E, stir evenly, and then discharge to obtain the emulsion.

[0040] The present invention also provides a blank application example of emulsion, which differs from the emulsion described in application example 1 only in that it does not contain an oil-controlling and moisturizing composition for oily and sensitive skin, and the missing amount is made up with an equal amount of deionized water. The remaining steps and parameters are the same as those in application example 1, and the blank application example of emulsion is prepared.

[0041] Example 1: Product oil control, moisturizing, and repair effects test The emulsions prepared using Application Examples 1-5, Comparative Application Examples 1-10, and the blank application example were used as test samples to test their oil control, moisturizing, and repairing effects. The methods are as follows: Select for oily, sensitive skin aged 18-45 (skin with an oil content of ≥120μg / cm² on the forehead within 8 hours before product use). 2 Eighty volunteers (who tested positive for lactic acid stinging) were enrolled. Subjects sat quietly for 30 minutes in a constant temperature and humidity room at 21±1℃ and 50±10% humidity. Initial facial oil content, moisture content, and TEWL (transepidermal water loss) values ​​were measured using a Sebumeter (SM815, Courage and Khazaka), a Corneometer® CM 825 (Courage + Khazaka) moisture probe, and a Tewameter® TMHex transepidermal water loss probe, respectively. Subjects were randomly divided into 16 groups of 5 people each. Each group used the product of this invention in equal amounts. After 7 days of use, facial oil content, moisture content, and TEWL values ​​were measured. The improvement of each indicator before and after product use was analyzed, and the improvement was expressed as the improvement rate of each indicator.

[0042] The improvement rates for each indicator are calculated as follows: Oil improvement rate % = (Oil content before use - Oil content after use) / Oil content before use × 100%; Moisture improvement rate % = (Moisture content after use - Moisture content before use) / Moisture content before use × 100%; TEWL improvement rate % = (TEWL value before use - TEWL value after use) / TEWL value before use × 100%; The higher the improvement rate, the stronger the oil control, moisturizing, and repair capabilities; the specific test results are shown in Table 6 below.

[0043] Table 6 Blank application example 5.5 8.3 1.7 Application Example 1 40.1 59.8 24.9 Application Example 2 35.9 54.6 22.1 Application Example 3 37.2 55.4 23.2 Application Example 4 31.6 47.5 17.5 Application Example 5 33.8 49.9 19.4 Comparative Application Example 1 14.4 23.2 3.8 Comparative Application Example 2 11.5 21.3 5.6 Comparative Application Example 3 13.9 19.7 5.3 Comparative Application Example 4 12.7 22.6 4.5 Comparative Application Example 5 18.3 28.1 9.7 Comparative Application Example 6 19.8 29.8 8.9 Comparative Application Example 7 17.6 26.5 7.2 Comparative Application Example 8 15.4 25.3 8.5 Comparative Application Example 9 17.1 24.6 7.9 Comparative Application Example 10 16.2 27.4 6.7 As shown in Table 6, compared with the comparative application example, the lotion containing an oil-controlling and moisturizing composition for oily and sensitive skin provided in the application example has good oil-controlling, moisturizing and repairing effects.

[0044] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An oil-controlling and moisturizing composition for oily and sensitive skin, characterized in that, The raw materials of the oil-controlling and moisturizing composition include Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract, and the mass ratio of Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract is (0.01-1):(0.1-2):(0.2-3):(0.01-2).

2. The oil-controlling and moisturizing composition for oily and sensitive skin according to claim 1, characterized in that, The mass ratio of the Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract is (0.1-0.5):(0.5-1.5):(0.5-2):(0.1-1).

3. The method for preparing the oil-controlling and moisturizing composition for oily and sensitive skin according to claim 1 or 2, characterized in that, The preparation method of the oil-controlling and moisturizing composition includes the following steps: The Lactobacillus / black mustard seed fermentation product extract, Norway spruce leaf extract, palmate kelp extract, and flaxseed extract were mixed evenly to obtain the oil-controlling and moisturizing composition.

4. The application of the oil-controlling and moisturizing composition for oily and sensitive skin according to claim 1 or 2 in the preparation of cosmetics, characterized in that, The cosmetics include toners, lotions, creams, masks, serums, and sprays.

5. The application according to claim 4, characterized in that, The amount of the oil-controlling and moisturizing composition for oily and sensitive skin added to the cosmetic is 0.5wt%-8wt%.

6. An emulsion, characterized in that, The composition containing the oil-controlling and moisturizing composition for oily and sensitive skin as described in claim 1 or 2.

7. The emulsion according to claim 6, characterized in that, It also contains excipients acceptable in the cosmetics industry.

8. The emulsion according to claim 7, characterized in that, The excipients acceptable in the cosmetics field include at least one of solvents, humectants, pH adjusters, emollients, emulsifiers, thickeners, solubilizers, antioxidants, chelating agents, and fragrances.

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