Tightening and nourishing composition and application thereof in essence oil

Abstract

The invention provides a firming and nourishing composition and application thereof in essence oil, and relates to the technical field of cosmetics. The firming and nourishing composition comprises coconut fruit extract, sea-buckthorn extract, capparis spinosa fruit extract and flaxseed extract. The composition obtained by compounding the extracts through multiple experimental researches has excellent tightening and nourishing effects, is mild and non-irritating, and has a good popularization and application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to a firming and nourishing composition and application thereof in essence oil. BACKGROUND

[0002] Skin relaxation and aging are closely related to the degradation of elastic fibers, which are composed of elastin and microfibrils. The degradation of elastic fibers is mainly catalyzed by elastase. Over-activation of elastase can lead to collagen loss, and further cause skin relaxation, wrinkles and other problems. In the prior art, cosmetics for skin firming and nourishing are mainly focused on a single target (such as simply inhibiting elastase or promoting collagen synthesis), which has limited efficacy and is prone to cause skin irritation. In addition, some components have intolerance risk for sensitive skin, and there is an urgent need for a safe, multi-target synergistic composition to comprehensively improve skin elasticity, barrier function and anti-aging needs. SUMMARY

[0003] To solve the problems in the prior art, the present application provides a firming and nourishing composition and application thereof in essence oil. The firming and nourishing composition comprises coconut fruit extract, sea buckthorn extract, capparis zeylanica fruit extract and flaxseed extract. The composition has excellent firming and nourishing effects on the skin through multi-target synergistic effect, can effectively improve the proliferation rate of HaCaT cells, inhibit the activity of elastase, and increase the secretion of collagen type I, and is suitable for sensitive skin.

[0004] The technical scheme adopted by the present application to achieve the above-mentioned purposes is as follows: In a first aspect, the present application provides a firming and nourishing composition, which comprises the following components by mass fraction: Coconut fruit extract: 8-10 parts; Sea buckthorn extract: 0.2-0.8 parts; Capparis zeylanica fruit extract: 1-8 parts; Flaxseed extract: 0.1-0.4 parts.

[0005] Among them: The coconut fruit extract is rich in coconut polysaccharides and phenolic compounds. The coconut polysaccharides can activate related pathways, stimulate the synthesis and proliferation of HaCaT cells, and up-regulate the expression of cell adhesion factors. The phenolic compounds can significantly scavenge free radicals, inhibit the activation of elastase by oxidative stress, and reduce collagen degradation.

[0006] The sea buckthorn extract is rich in flavonoids, vitamin C, superoxide dismutase (SOD) and other antioxidants, which can scavenge active oxygen and reduce oxidative stress, reduce the expression of elastase, and delay collagen degradation.

[0007] The Capparis zeylanica fruit extract contains alkaloids and flavonoids, wherein the alkaloids include stachydrine hydrochloride compounds, which can promote the differentiation of fibroblasts into collagen synthesis type, and up-regulate the expression of collagen type I; and the flavonoids can inhibit matrix metalloproteinases and reduce the degradation of collagen type I.

[0008] The flaxseed extract contains oligosaccharides and amino acids, wherein the oligosaccharides can promote the migration and differentiation of fibroblasts and enhance collagen synthesis, and the amino acid compounds such as flaxseed cyclic peptides can up-regulate the secretion of collagen type I through related pathways, neutralize free radicals and inhibit oxidative stress, thereby reducing the release of inflammatory mediators and relieving sensitive symptoms such as redness and burning, and in addition, the flaxseed extract can stimulate skin cell regeneration and enhance skin barrier.

[0009] The present application researches and finds that the combination of the above components has synergistic effect and can achieve excellent firming and nourishing effects.

[0010] As a preferred embodiment of the firming and nourishing composition of the present application, the composition comprises the following components by mass fraction: Cocos nucifera fruit extract: 9-10 parts; Sea buckthorn extract: 0.5-0.8 parts; Capparis zeylanica fruit extract: 4-8 parts; Flaxseed extract: 0.2-0.4 parts.

[0011] The present application researches and finds that the mass fraction of each component in the composition will affect the performance of the composition, and when the mass fraction of the components is further selected within the above range (including the end point value), the performance of the obtained composition is better.

[0012] As the most preferred embodiment of the firming and nourishing composition of the present application, the composition comprises the following components by mass fraction: Cocos nucifera fruit extract: 9 parts; Sea buckthorn extract: 0.5 parts; Capparis zeylanica fruit extract: 4 parts; Flaxseed extract: 0.2 parts.

[0013] The present application researches and finds that the mass fraction of each component in the composition will affect the performance of the composition, and when the mass fraction of the components is further selected within the above range (including the end point value), the performance of the obtained composition is better.

[0014] In a second aspect, the present application provides the use of the firming and nourishing composition of the first aspect in the preparation of a cosmetic product with firming and nourishing effects.

[0015] Preferably, the dosage form of the cosmetic product with firming and nourishing effects is at least one of an essence oil, a cream, and a spray.

[0016] Thirdly, the present invention provides an essential oil with firming and nourishing effects, the essential oil comprising 1-10 wt% of the firming and nourishing composition described in the first aspect.

[0017] Preferably, the essential oil with firming and nourishing effects also includes base oils and antioxidants.

[0018] Preferably, the base oil is at least one of jojoba oil, sunflower seed oil, hazelnut oil, olive oil, and camellia seed oil.

[0019] Preferably, the antioxidant is at least one of vitamin E and pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester.

[0020] Preferably, the method for preparing the essential oil includes the following steps: S1: Mix the firming and nourishing composition with 1 / 2 of the base oil until homogeneous to obtain mixture A; S2: Mix the antioxidant with 1 / 4 of the base oil until homogeneous to obtain mixture B; S3: Mix mixture A, mixture B, and the remaining base oil evenly, then fill into bottles to obtain the essential oil.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention utilizes a combination of coconut fruit extract, sea buckthorn extract, capernaum fruit extract, and flaxseed extract to create a firming and nourishing composition that inhibits elastase, promotes collagen secretion, and enhances HaCaT cell proliferation. Regarding elastase inhibition, the vitamin C / SOD system in sea buckthorn extract and the phenolic compounds in coconut fruit extract achieve dual inhibition of elastase through upstream antioxidant intervention and downstream enzyme activity blocking, respectively. Capernaum fruit extract and flaxseed extract synergistically enhance elastase activity through direct inhibition and inflammatory regulation, respectively. Regarding collagen secretion, capernaum fruit extract activates the TGF-β / Smad pathway to promote collagen synthesis, while flaxseed extract enhances fiber assembly through the MAPK / ERK pathway. Coconut fruit extract and sea buckthorn extract form a synthesis-modification closed loop from the perspectives of cell nutrition supply and collagen structure stabilization, respectively. Regarding HaCaT cell proliferation, coconut fruit extract activates the PI3K / Akt pathway to accelerate cell division, flaxseed extract upregulates EGFR to promote migration, and sea buckthorn extract and capernaum fruit extract create a low-inflammatory environment. These three components work together to form a positive feedback loop of "division-migration-protection." Ultimately, through a three-dimensional synergistic network of degradation inhibition, synthesis promotion, and repair promotion, significant synergistic effects are achieved in key indicators such as inhibiting elastase activity, promoting type I collagen secretion, and accelerating cell migration, breaking through the functional limitations of single components. Detailed Implementation

[0022] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terms "composition" and "essential oil" used are for describing specific specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0023] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by percentage and parts by mass.

[0024] Some of the raw materials and their sources are as follows: Coconut fruit extract: Shanghai Boshuo Industrial Co., Ltd.; Sea buckthorn extract: Guangzhou Puen Chemical Co., Ltd.; Caprifolia fruit extract: Guangzhou Puen Chemical Co., Ltd.; Flaxseed extract: Guangzhou Bino Biotechnology Co., Ltd.

[0025] All other raw materials and reagents are commercially available.

[0026] The firming and nourishing composition has the following components and parts by weight: Firming and Nourishing Composition 1: Coconut fruit extract: 9 parts; Sea buckthorn extract: 0.5 parts; Caprifolia fruit extract: 4 parts; Flaxseed extract: 0.2 parts; The preparation method of the firming and nourishing composition 1 includes the following steps: accurately weigh each component, mix them evenly, and store them in a light-proof and sealed container.

[0027] Firming and Nourishing Composition 2: Coconut fruit extract: 10 parts; Sea buckthorn extract: 0.8 parts; Caprifolia fruit extract: 8 parts; Flaxseed extract: 0.4 parts; The preparation method is the same as that of composition 1.

[0028] Firming and Nourishing Composition 3: Coconut fruit extract: 8 parts; Sea buckthorn extract: 0.2 parts; Caprifolia fruit extract: 1 part; Flaxseed extract: 0.1 parts; The preparation method is the same as that of composition 1.

[0029] Firming and nourishing composition ①: Unlike composition 1, this composition lacks coconut fruit extract. The missing mass fraction is made up by sea buckthorn extract, capercaillie fruit extract, and flaxseed extract in a mass ratio of 0.5:4:0.2. The preparation method is the same as that of composition 1.

[0030] Firming and nourishing composition ②: Unlike composition 1, it lacks sea buckthorn extract, and the missing mass fraction is made up by coconut fruit extract, capercaillie fruit extract and flaxseed extract in a mass ratio of 9:4:0.2; the preparation method is the same as that of composition 1.

[0031] Firming and nourishing composition ③: Unlike composition 1, this composition lacks capernaum fruit extract, and the missing mass fraction is made up by coconut fruit extract, sea buckthorn extract, and flaxseed extract in a mass ratio of 9:0.5:0.2; the preparation method is the same as that of composition 1.

[0032] Firming and nourishing composition ④: Unlike composition 1, this composition lacks flaxseed extract, and the missing mass fraction is made up by coconut fruit extract, sea buckthorn extract, and capernaum fruit extract in a mass ratio of 9:0.5:4; the preparation method is the same as that of composition 1.

[0033] Firming and nourishing composition ⑤: Coconut fruit extract: 0.5 parts; Sea buckthorn extract: 4 parts; Caprifolia fruit extract: 0.2 parts; Flaxseed extract: 9 parts; The preparation method is the same as that of composition 1.

[0034] The components and weight percentages of the firming and nourishing essential oil are as follows: Essential Oil 1: Firming and nourishing composition 1:5wt% Antioxidant: 0.5 wt%; Base oils: Add to 100 wt%; The antioxidant is vitamin E, and the base oil is olive oil.

[0035] Essential Oil 2: Firming and nourishing composition 1:1wt% Antioxidant: 0.5 wt%; Base oils: Add to 100 wt%; The antioxidant is vitamin E, and the base oils are hazelnut oil and camellia seed oil in a 1:1 mass ratio.

[0036] Essential Oil 3: Firming and nourishing composition 1: 10wt% Antioxidant: 0.5 wt%; Base oils: Add to 100 wt%; The antioxidant is pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, and the base oil is sunflower seed oil and jojoba oil in a mass ratio of 2:1.

[0037] Essential Oil 4: Unlike essence oil 1, firming and nourishing composition 2 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as essence oil 1.

[0038] Essential Oil 5: Unlike essence oil 1, firming and nourishing composition 3 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as those in essence oil 1.

[0039] Comparison of essential oil 1: Unlike essence oil 1, firming and nourishing composition 1 is replaced by a firming and nourishing composition ① by the same mass, while the remaining components and mass percentages are the same as those in essence oil 1.

[0040] Comparison with essential oil 2: Unlike essence oil 1, firming and nourishing composition 2 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as those of essence oil 1.

[0041] Comparison with essential oil 3: Unlike essence oil 1, firming and nourishing composition 3 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as those of essence oil 1.

[0042] Comparison with essential oil 4: Unlike essence oil 1, firming and nourishing composition 4 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as those of essence oil 1.

[0043] Comparison of essential oil 5: Unlike essence oil 1, firming and nourishing composition 5 is used to replace firming and nourishing composition 1 by the same mass, while the remaining components and mass percentages are the same as those in essence oil 1.

[0044] Blank comparison of essential oils: Antioxidant: 0.5 wt%; Base oils: Add to 100 wt%; The antioxidant is vitamin E, and the base oil is olive oil.

[0045] The preparation method of the above-mentioned essential oil 1 is as follows: S1: Mix the firming and nourishing composition with 1 / 2 of the base oil until homogeneous to obtain mixture A; S2: Mix the antioxidant with 1 / 4 of the base oil until homogeneous to obtain mixture B; S3: Mix mixture A, mixture B, and the remaining base oil evenly, then fill into bottles to obtain the essential oil.

[0046] The preparation methods for essential oils 2-5 and comparative essential oils 1-5 are the same as those for essential oil 1.

[0047] The preparation method of blank essential oil is as follows: S1: Mix the antioxidant with 1 / 4 of the base oil until homogeneous to obtain mixture A; S2: Mix the mixture A with the remaining base oils evenly, fill the bottle, and obtain the essential oil.

[0048] Experiment 1: Effect of the composition on elastase Skin laxity and aging are mainly related to elastic fibers, which are composed of elastin and microfibrils. Elastic fibers can be broken down by elastase, resulting in collagen loss and thus skin laxity and aging. Therefore, the firming and anti-wrinkle effects of a firming and nourishing composition can be evaluated by measuring the inhibition rate of elastase on the composition.

[0049] The specific procedure was as follows: 100 μL of 0.2 mol / L tris-HCl buffer, 25 μL of 10 mmol / L N-methoxysuccinyl-alanine-proline-valine-4-nitroaniline, and 30 mg of the firming and nourishing composition were mixed and incubated at 25 °C for 10 min. Then, 25 μL of 0.3 U / mL elastase was added and the mixture was incubated for another 10 min. Finally, the OD value was measured at 410 nm. A blank control was prepared by replacing the firming and nourishing composition with an equal volume of tris-HCl buffer. The results are shown in Table 1 below.

[0050] The inhibition rate of the firming and nourishing composition against elastase (%) = (OD value of blank control - OD value of firming and nourishing composition) / OD value of blank control group × 100%.

[0051] Table 1. Verification of the firming and anti-wrinkle effects of the firming and nourishing composition. Group Elastase inhibition rate / % Tightening and nourishing composition 1 42.55 Tightening and nourishing composition 2 41.17 Tightening and nourishing composition 3 40.31 Tightening and nourishing composition 1 34.26 Tightening and nourishing composition 2 31.51 Tightening and nourishing composition 3 29.32 Tightening and nourishing composition 4 33.64 Tightening and nourishing composition 5 38.82 According to the results of Firming and Nourishing Compositions 1-3 and 1-5 in Table 1, the firming and nourishing compositions defined in this invention have a strong inhibitory effect on elastase, indicating that they have certain firming and anti-wrinkle effects. According to the results of Firming and Nourishing Composition 1 and Firming and Nourishing Compositions 1-5, the four raw materials defined in the compositions of this invention, when used together in the mass proportions defined in this invention, have the best effect on improving the elastase inhibition rate, indicating that the four raw materials in the compositions of this invention have a significant synergistic effect. According to the results of Firming and Nourishing Compositions 1-3 and Firming and Nourishing Composition 5, the raw materials in the compositions defined in this invention, when used in combination within the mass proportions defined in this invention, have the best effect on improving the elastase inhibition rate.

[0052] Experiment 2: Effect of the composition on type I collagen secretion by fibroblasts Type I collagen is one of the main components of the extracellular matrix of dermal cells. It is synthesized intracellularly by dermal fibroblasts, secreted extracellularly, and then polymerized to form collagen fibers under the action of terminal procollagen peptidase, after the telopeptides are separated. Human dermal fibroblasts can be used as a cell model to study the effect of test substances on increasing type I collagen content and to evaluate whether the test substances are effective in promoting collagen synthesis.

[0053] Test substances: Firming and nourishing compositions 1-3, firming and nourishing compositions ①-⑤.

[0054] Cell digestion and seeding: Commercially available human skin fibroblasts (Beina Biotechnology, catalog number: BNCC341540) were digested with trypsin / EDTA until most cells became rounded and were in suspension. Digestion was terminated by adding twice the volume of trypsin-containing DMEM medium containing serum, and the cells were collected and centrifuged at 1000 rpm for 5 min. After centrifugation, the supernatant was discarded, and the cells were resuspended in cell culture medium. The cell concentration was adjusted to the seeding density (confluence reached 45%–60% 24 h post-seeding). 100 μL of cells were seeded into each well of a 96-well plate and incubated in a CO2 incubator for 24 h ± 2 h.

[0055] (1) Drug administration: Discard the culture medium in the 96-well plate, dilute the test substance to a volume fraction of 1% with cell culture medium and add it to the test substance wells. Add 100 ng / mL TGFβ1 medium to the positive control wells and cell culture medium to the blank control wells. 200 μL of medium is added to each well, and each test substance corresponds to 3 replicates. After drug administration, place the 96-well plate in a CO2 incubator and incubate for 24 h ± 2 h.

[0056] (2) ELISA detection: After the culture is completed, the cell culture supernatant in each well is collected and the detection is performed according to the instructions of the human type I collagen (ColⅠ) ELISA kit (Shanghai Enzyme Link, ml057630).

[0057] (3) Calculation of type I collagen upregulation rate: The type I collagen content of the test wells was calculated according to the kit instructions. The average value of the three replicates of each group was taken as the average value of type I collagen content of that group. The type I collagen upregulation rate was calculated using the formula for type I collagen upregulation rate.

[0058] Formula for calculating the upregulation rate of type I collagen: Upregulation rate (%) = (T / C-1) × 100%, Where: T—average content of type I collagen in the test substance; C—average content of type I collagen in the blank control.

[0059] The experimental results are shown in Table 2.

[0060] Table 2. Verification of the firming and anti-wrinkle effects of the firming and nourishing composition. Group Up-regulation rate / % Tightening and nourishing composition 1 39.44 Tightening and nourishing composition 2 35.93 Tightening and nourishing composition 3 37.62 Tightening and nourishing composition 1 24.39 Tightening and nourishing composition 2 22.53 Tightening and nourishing composition 3 19.41 Tightening and nourishing composition 4 21.47 Tightening and nourishing composition 5 31.26 Positive control 47.31 According to the results of Firming and Nourishing Compositions 1-3 and 1-5 in Table 2, the firming and nourishing compositions defined in this invention have a strong effect on upregulating the secretion of type I collagen, indicating that they have certain firming and anti-wrinkle effects. According to the results of Firming and Nourishing Composition 1 and Firming and Nourishing Compositions 1-5, the four raw materials defined in the composition of this invention, when used together in the mass proportions defined in this invention, have the best effect on increasing the content of type I collagen, indicating that the four raw materials in the composition of this invention have a significant synergistic effect. According to the results of Firming and Nourishing Compositions 1-3 and Firming and Nourishing Composition 5, the raw materials in the composition defined in this invention, when used together in the mass proportions defined in this invention, have the best effect on increasing the content of type I collagen.

[0061] Experiment 3: Validation of the efficacy of the composition in promoting HaCaT cell migration The degree of cell migration has a significant impact on wound healing and skin repair. During wound healing, cell migration is a crucial step in the process of epidermal cells covering the wound; therefore, the higher the cell migration rate, the faster the wound heals. Simultaneously, cell migration also participates in the repair process after skin injury; impaired cell migration can lead to hindered skin repair.

[0062] Test sample: Prepare a 1wt% mixture of firming and nourishing compositions 1-3 and ①-⑤ using DMSO. Prepare as needed.

[0063] Press 1×10 6HaCaT cells were seeded at a density of 1 unit into 12-well plates and incubated overnight at 37°C with 5% CO2. When the cell deposition rate reached approximately 80%, the cells were divided into a sample group and a control group. Both groups were scratched. After scratching, the cells were washed with PBS to remove dead cells, and then fresh SCF-free keratinocyte culture medium was added. The sample group received 50 μL of the experimental sample per well, while an equal volume of DMSO served as the control group. The cells were photographed and recorded using an inverted microscope. The cells were incubated at 37°C with 5% CO2 for 24 hours, and photographed again. Statistical analysis was performed using ImageJ software. Each group (sample and control) had three parallel experiments. The average cell migration rate was calculated using the following formula, and the results are shown in Table 3.

[0064] Cell migration rate / % = (scratch area before experiment - scratch area after experiment) / scratch area before experiment × 100%.

[0065] Average cell migration rate (%) = Sum of cell migration rates in parallel experiments for the same sample / Number of parallel experiments.

[0066] Table 3. Verification of the Repairing Efficacy of the Firming and Nourishing Composition Group Cell average migration rate / % Tightening and nourishing composition 1 24.26 Tightening and nourishing composition 2 23.17 Tightening and nourishing composition 3 23.52 Tightening and nourishing composition 1 18.21 Tightening and nourishing composition 2 17.31 Tightening and nourishing composition 3 14.62 Tightening and nourishing composition 4 16.57 Tightening and nourishing composition 5 20.94 Blank control 1.13 According to the results of the firming and nourishing compositions 1-3, 1-5, and the blank control in Table 3, the compositions defined in this invention can significantly improve the migration rate of HaCaT cells, indicating that they have a certain repair effect. According to the results of firming and nourishing compositions 1 and 1-5, the four raw materials defined in the compositions of this invention, when used together in the mass proportions defined in this invention, have the best effect on improving cell migration rate, indicating that the four raw materials in the compositions of this invention have a significant synergistic effect. According to the results of firming and nourishing compositions 1-3 and 5, the raw materials in the compositions defined in this invention, when used together in the mass proportions defined in this invention, have the best effect on improving cell migration rate.

[0067] Experiment 4: Human Efficacy Test Experiment A: The irritation of cosmetics was evaluated using the 2015 "Cosmetic Safety Technical Specifications" as a reference standard. The test method was a skin patch test. Volunteers aged 16-65 years were randomly distributed and tested. Volunteers were divided into groups according to the number of test substances, with each group corresponding to one test substance and 32 people in each group.

[0068] Test substances: essential oils 1-5, control essential oils 1-5, and blank essential oil.

[0069] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the volunteer's back or the flexor side of the forearm with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator 30 minutes after the pressure mark has disappeared and observe the skin reaction. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0070] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0071] Test results: All volunteers had negative skin reactions.

[0072] Experiment B: Transdermal water loss (TEWL) is an important parameter for assessing skin barrier function. It represents the loss of moisture from the deep dermis through evaporation from the epidermis. A higher TEWL indicates that more moisture is lost through the skin, and the weaker the skin's moisturizing ability.

[0073] Test samples: essential oils 1-5, control essential oils 1-5, and blank essential oil.

[0074] Transepidermal water loss was measured using a transepidermal water loss (TEWL) tester, specifically a TM300 instrument from Courage & Khazaka, Germany. For each test sample, 32 women aged 20 to 40 without skin diseases were randomly selected as subjects. Subjects took the test sample (0.5 ml) twice a day, morning and evening, and applied it evenly to the inner forearm. The same tester then measured the transepidermal water loss (TEWL value) of the area where the test sample was applied after week 0 and week 4. The average decrease rate of TEWL value was calculated using the following formula, and the results are shown in Table 4 below.

[0075] Formula: TEWL decrease rate / % = A0− ; In the formula: A0 is the TEWL value of week 0; A4 is the TEWL value of week 4.

[0076] A positive TEWL value decrease rate indicates that the skin barrier has been repaired, while a negative TEWL value decrease rate indicates that the skin barrier has not been repaired. Furthermore, the greater the TEWL value decrease rate, the better the skin barrier repair effect.

[0077] The formula for calculating the average rate of decline of the TEWL value is as follows: Average TEWL value decline rate (%) = Sum of TEWL value decline rates for each group / Number of people in that group.

[0078] Table 4 Average rate of decrease in TEWL values Group Test number / person TEWL average decrease rate / % Essence oil 1 32 17.62 Essence oil 2 32 12.17 Essence oil 3 32 18.54 Essence oil 4 32 16.81 Essence oil 5 32 17.14 Comparative essence oil 1 32 14.19 Comparative essence oil 2 32 13.26 Comparative essence oil 3 32 11.25 Comparative essence oil 4 32 13.53 Comparative essence oil 5 32 15.82 Blank essence oil 32 4.22 As shown in Table 4, the results of the experiments indicate that, compared with the blank essence oil and essence oils 1-5, and comparing the results of essence oils 1-5, the firming and nourishing composition provided by this invention has a significant effect on increasing the TEWL reduction rate, indicating that it has a significant skin repair effect. Specifically, according to the results of essence oils 1-3, the amount of firming and nourishing composition added can affect the TEWL reduction rate; according to the results of essence oil 1 and comparative essence oils 1-4, there is a significant synergistic effect among the raw materials in the firming and nourishing composition, and the repair effect is significantly reduced if any one of them is missing; according to the results of essence oils 1, 4, 5 and comparative essence oil 5, the ratio of each raw material in the firming and nourishing composition of this invention has a certain impact on the repair effect, and the firming and nourishing composition composed of raw materials within the range defined by this invention has the most significant effect on increasing the average TEWL reduction rate.

[0079] Experiment C: Test samples: essential oils 1-5, control essential oils 1-5, and blank essential oil.

[0080] For each test sample, 32 women aged 30 to 45 with wrinkles and no skin diseases were randomly selected as subjects. Subjects took the test sample (0.5 ml) twice a day, morning and evening, and applied it evenly to their face for 30 consecutive days. During the test, no other cosmetics could be applied.

[0081] Data were collected using a Cutometer MPA580 skin elastometer on day 0 and day 30, with the data collection site being the cheekbone area. During measurement, the probe was used to measure the same area three times, and the average value was taken.

[0082] The test parameter is R2 (the ratio of skin rebound Ua without negative pressure to the maximum stretch Uf with negative pressure). The closer R2 is to 1, the better the skin elasticity. R2 change rate (%) = (R2 value on day 30 - R2 value on day 0) / R2 value on week 0 × 100%.

[0083] Table 5 Results of Skin Elasticity Test Group Test number / person R2 (day 0) R2 (day 30) R2 change rate (%) Essence oil 1 32 0.538 0.609 13.20 Essence oil 2 32 0.522 0.568 8.81 Essence oil 3 32 0.529 0.605 14.37 Essence oil 4 32 0.534 0.599 12.17 Essence oil 5 32 0.537 0.605 12.66 Comparative essence oil 1 32 0.535 0.579 8.22 Comparative essence oil 2 32 0.541 0.578 6.84 Comparative essence oil 3 32 0.524 0.555 5.92 Comparative essence oil 4 32 0.521 0.559 7.29 Comparative essence oil 5 32 0.537 0.593 10.43 Blank essence oil 32 0.544 0.554 1.84 As shown in Table 5, the results of the experiments indicate that, compared with the blank essence oil and essence oils 1-5, and comparing the results of essence oils 1-5, the firming and nourishing composition provided by this invention has a significant effect on improving skin elasticity. Specifically, according to the results of essence oils 1-3, the amount of firming and nourishing composition added can affect the effect of improving skin elasticity; according to the results of essence oil 1 and comparative essence oils 1-4, there is a significant synergistic effect among the raw materials in the firming and nourishing composition, and the lack of any one of them significantly reduces the effect of improving skin elasticity; according to the results of essence oils 1, 4, 5 and comparative essence oil 5, the ratio of each raw material in the firming and nourishing composition of this invention has a certain impact on the repair effect, and the firming and nourishing composition composed of raw materials within the range defined by this invention has the most significant effect on improving skin elasticity.

[0084] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A firming and nourishing composition, characterized in that, The composition comprises the following components in parts by weight: Coconut fruit extract: 8-10 parts; Sea buckthorn extract: 0.2-0.8 parts; Caprifolia fruit extract: 1-8 parts; Flaxseed extract: 0.1-0.4 parts.

2. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: Coconut fruit extract: 9-10 parts; Sea buckthorn extract: 0.5-0.8 parts; Caprifolia fruit extract: 4-8 parts; Flaxseed extract: 0.2-0.4 parts.

3. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight; Coconut fruit extract: 9 parts; Sea buckthorn extract: 0.5 parts; Caprifolia fruit extract: 4 parts; Flaxseed extract: 0.2 parts.

4. The use of the composition according to any one of claims 1-3 in the preparation of cosmetics with firming and nourishing effects.

5. The application according to claim 4, characterized in that, The dosage form of the cosmetic with firming and nourishing effects is at least one of essential oil, face cream, and spray.

6. An essential oil with firming and nourishing effects, characterized in that, The essential oil comprises 1-10 wt% of the firming and nourishing composition according to any one of claims 1-3.

7. The essential oil according to claim 6, characterized in that, It also includes base oils and antioxidants.

8. The essential oil according to claim 7, characterized in that, The base oil is at least one of jojoba oil, sunflower seed oil, hazelnut oil, olive oil, and camellia seed oil.

9. The essential oil according to claim 7, characterized in that, The antioxidant is at least one of vitamin E and pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester.

10. The essential oil according to any one of claims 7-9, characterized in that, The method for preparing the essential oil includes the following steps: S1: Mix the firming and nourishing composition with 1 / 2 of the base oil until homogeneous to obtain mixture A; S2: Mix the antioxidant with 1 / 4 of the base oil until homogeneous to obtain mixture B; S3: Mix mixture A, mixture B, and the remaining base oil evenly, then fill into bottles to obtain the essential oil.