Composition for gently glowing skin and efficiently removing acnes and application thereof

Abstract

The invention discloses a mild skin-refreshing and efficient acne-removing composition and application thereof, and relates to the technical field of cosmetics. The composition comprises a component A, a component B and a component C, the component A comprises a hydrolyzed opuntia ficus-indica flower extract and a guaiacol extract; the component B comprises pomelo seed oil and rosa multiflora water, and the component C is papain. The combination of the hydrolyzed opuntia ficus-indica flower extract, the pomelo seed oil, the rosa multiflora water, the papain and the guaiacol extract finds that the composition has a synergistic interaction effect, and the composition formed by the components through specific compatibility has the effects of removing cutin, regulating water-oil balance, protecting skin barrier and improving acne; particularly, when being applied to sensitive skin, the composition can be used for mildly exfoliating, is high in subject satisfaction degree and high in acceptability, and can be repeatedly used for a long time.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a mild skin rejuvenating and highly effective acne removing composition and application thereof. Background Art

[0002] The excessive proliferation of keratinocytes in acne patients causes the epithelial cell layer of the sebaceous gland duct to continue to thicken, the diameter to decrease, and the patency to weaken, which eventually leads to acute occlusion of the hair follicle sebaceous gland duct, and the hair follicles to bulge and form acne. The inhibition of sebum secretion provides a good anaerobic environment for Propionibacterium acnes, leading to secondary inflammatory acne. Under electron microscope observation, the number and volume of transparent keratin particles in the keratinized area can be seen to increase, and lipid droplets accumulate.

[0003] In addition, Propionibacterium acnes and its cell wall components induce keratinocytes to secrete IL-1, which leads to TLR-2 activation, which is the initial step of hyperkeratinization of the sebaceous glands of the hair follicles. TLR-2 can not only induce keratinocytes to secrete IL-1, IL-6, IL-8 or TNF-α, but also promote macrophages to express IL-8 and IL-12, resulting in hyperkeratinization, dry and flaky skin, and damaged skin barrier, which damages skin health.

[0004] Chemical exfoliation is a popular method nowadays. It involves applying acidic chemicals to the surface of the skin, causing controllable damage to the epidermis and / or dermis, removing old keratin, and then promoting skin repair and regeneration. The type, concentration, and action time of acidic chemicals will affect the depth of chemical peeling. According to the depth of peeling, it can be divided into shallow, middle, and deep peeling. The deeper the peeling depth, the more obvious the effect, but the greater the possibility of adverse reactions. In addition, not all skin is suitable for acid peeling. People with sensitive skin, thin stratum corneum, etc. need to be extra cautious. Improper use can trigger acne, make the skin barrier more fragile, and damage skin health. Therefore, seeking a product that can gently exfoliate and remove acne has become a technical problem that needs to be solved in this field. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a composition for gentle skin rejuvenating and efficient acne removal and its application. The composition has the effects of gentle exfoliation, regulating water-oil balance, protecting skin barrier and improving acne. It is especially used on sensitive skin, can gently exfoliate, has high subject satisfaction and strong acceptance, and can be used repeatedly and for a long time.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] In a first aspect, the present invention provides a composition for gentle skin rejuvenation and efficient acne removal, the composition comprising component A, component B and component C; component A comprises hydrolyzed prickly pear cactus flower extract and guaiac wood extract; component B comprises grapefruit seed oil and rose flower water; and component C is papain.

[0008] Hydrolyzed Opuntia fruticosa flower extract is a natural plant extract extracted from the flowers of Opuntia fruticosa (OPUNTIAFICUS-INDICA) through a hydrolysis process. The main active ingredients may include polysaccharides, proteins, amino acids, natural phenolic compounds, flavonoids, vitamin C, minerals, etc. Hydrolyzed Opuntia fruticosa flower extract is rich in polysaccharides and has strong moisturizing properties. Polysaccharides can help the skin retain moisture and enhance the skin's moisturizing ability by combining with water molecules to form a hydration layer. Proteins and amino acids are the basis of skin repair and renewal, and can provide the basic nutrients needed by the skin and help enhance the skin's barrier function.

[0009] Guaiacum officinale Extract is derived from Guaiacum officinale and its related species. Guaiacum officinale is widely used in traditional medicine, especially in the treatment of skin diseases. Its extract is rich in bioactive ingredients, mainly including guaiacol and its derivatives, flavonoids and phenolic compounds. Guaiacum officinale extract is rich in natural moisturizing factors, which can provide lasting moisture to the skin, help maintain the water and oil balance of the skin, and have a certain moisturizing and soothing effect; the active ingredients in guaiacum officinale extract can promote the renewal of skin cells, help repair damaged skin tissue, promote skin repair, improve skin roughness, make the skin smoother and more delicate, and improve skin texture.

[0010] Grapefruit is very rich in pharmacological effects and nutritional value. Grapefruit (CITRUS GRANDIS) seed oil is an essential oil extracted from grapefruit seeds. Grapefruit seed oil contains a large amount of terpene compounds, which have antioxidant, anti-inflammatory, antibacterial and sedative effects. Grapefruit seed oil also contains a small amount of flavonoids and vitamin C. These ingredients have antioxidant, anti-aging and skin repair effects.

[0011] Rosa centifolia flower water is a natural plant water with a wide range of skin care benefits. The natural sugars, amino acids and minerals it contains can help the skin retain moisture and enhance the skin's ability to retain moisture. It can deeply moisturize the skin and keep it soft and smooth. Amino acids can also help repair damaged skin barriers and enhance the skin's ability to lock in moisture. Secondly, it has good soothing and calming effects, especially suitable for sensitive skin. Its aromatic ingredients (such as rose alcohol) have anti-inflammatory and calming effects, which help reduce skin redness, allergies and irritation. Rose alcohol and other aromatic compounds have natural antibacterial, antifungal and antiviral effects, which can remove bacteria, fungi and other microorganisms on the skin surface.

[0012] Papain is the main active ingredient in papaya and is an enzyme belonging to the cysteine ​​protein (Cysteineprotease) class. It can hydrolyze the peptide bonds of proteins, especially the cleavage of large molecular proteins. Papain can break down the proteins in the stratum corneum on the surface of the skin, especially the keratin in the stratum corneum. By breaking down these hardened keratin proteins into smaller fragments, papain helps remove dead skin cells and promotes the renewal of the surface layer of the skin.

[0013] The present invention has found that when the above-mentioned plant extracts are used in combination, they can synergistically achieve the effects of exfoliation, regulating water-oil balance, protecting the skin barrier and improving acne. Especially when applied to sensitive skin, they can gently exfoliate, with high satisfaction and strong acceptance among the subjects, and can be used repeatedly and for a long time.

[0014] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.1-6): (1.01-11): (0.01-0.1). Exemplarily, the mass ratio of component A, component B and component C in the composition can be any value of 1.1: 1.01: 0.01, 1.1: 1.5: 0.05, 4: 2: 0.05, 5: 4: 0.05, 6: 11: 0.1 or a range value between any values.

[0015] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.5-4): (2.05-8.8): (0.04-0.08).

[0016] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = 2:6.7:0.06.

[0017] The present invention has found that the mass ratio of component A, component B, and component C in the exfoliating and acne-removing composition will not only affect the scale index, the renewal ability of keratinocytes, and the inhibitory ability of papules and acne to a certain extent, thereby affecting the exfoliating and acne-removing effects of the composition, but also the skin barrier repair ability and moisturizing effect of the composition. When the mass ratio of component A, component B, and component C is further selected to be (1.1-6): (1.01-11): (0.01-0.1), especially (0.5-4): (2.05-8.8): (0.04-0.08), the exfoliating and acne-removing composition obtained has a more excellent effect on the renewal ability of keratinocytes and the inhibitory ability of papules and acne; the composition has a greater degree of enhancement of the renewal ability of keratinocytes and the inhibitory ability of acne papules, and the comprehensive ability of exfoliating, acne removal, moisturizing, and repairing of the composition is improved. It has been found through research that when the mass ratio of component A, component B and component C is 2:6.7:0.06, the overall comprehensive effect of the composition of the present invention is the best.

[0018] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed Opuntia pyrifolia flower extract to the Guaiac wood extract is hydrolyzed Opuntia pyrifolia flower extract:Guaiac wood extract=(0.1-3):(1-3). Exemplarily, the mass ratio of the hydrolyzed Opuntia pyrifolia flower extract to the Guaiac wood extract can be any value among 0.1:1, 0.1:1.5, 0.1:2, 1:1, 2:1, 3:1, 3:2, 0.1:3 or a range value between any values.

[0019] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed opuntia cactus flower extract to the guaiac extract is hydrolyzed opuntia cactus flower extract:guaiac extract=(0.3-2):(1.2-2).

[0020] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed opuntia cactus flower extract to the guaiac extract is hydrolyzed opuntia cactus flower extract:guaiac extract=0.5:1.5.

[0021] The present invention has been found that the mass ratio of the hydrolyzed opuntia cactus flower extract and the guaiac extract in component A of the exfoliating and acne-removing composition will affect the TEWL value and skin moisture content of the composition to a certain extent, thereby affecting the barrier repair and moisturizing ability of the composition. When the mass ratio of the hydrolyzed opuntia cactus flower extract and the guaiac extract is further selected to be (0.1-3):(1-3), especially (0.3-2):(1.2-2), the barrier repair and moisturizing ability of the composition can be improved. The study found that when the mass ratio of the hydrolyzed opuntia cactus flower extract and the guaiac extract is 0.5:1.5, the barrier repair and moisturizing ability of component A of the present invention is the best.

[0022] As a preferred embodiment of the first aspect, in the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water=(0.01-1):(1-10). Exemplarily, the mass ratio of grapefruit seed oil to Rosa centifolia flower water can be any value among 0.01:1, 0.01:2, 0.01:4, 0.01:6, 0.01:8, 0.01:10, 1:1, 1:4, 1:8, 1:10, or a range value between any values.

[0023] As a preferred embodiment of the first aspect, in the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water = (0.05-0.8): (2-8).

[0024] As a preferred implementation of the first aspect, in the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water=0.4:6.3.

[0025] The present invention has found that the mass ratio of grapefruit seed oil and Rosa centifolia flower water in component B of the exfoliating and acne-removing composition will affect the effect of the composition on inhibiting Propionibacterium acnes and the sedative effect to a certain extent, thereby affecting the acne-removing, anti-inflammatory and antibacterial abilities of the composition. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is further selected to be (0.01-1):(1-10), especially (0.05-0.8):(2-8), the acne-removing, anti-inflammatory and antibacterial abilities of the composition can be improved. It has been found that when the mass ratio of grapefruit seed oil to Rosa centifolia flower water is 0.4:6.3, the acne-removing, anti-inflammatory and antibacterial effects of component B of the present invention are the best.

[0026] As a preferred embodiment of the first aspect, the method for preparing grapefruit seed oil comprises the following steps:

[0027] (1) taking grapefruit seeds, drying them, crushing them, adding water and mixing them in a water bath;

[0028] (2) adding protease to perform enzymolysis to obtain an enzymolysis product;

[0029] (3) centrifuging the enzymatic hydrolyzate to break the emulsion, separating the emulsion layer, and centrifuging the emulsion layer to obtain free oil;

[0030] (4) drying the free oil to obtain the grapefruit seed oil.

[0031] As a preferred embodiment of the first aspect, the method for preparing grapefruit seed oil satisfies at least one of the following:

[0032] (a) the material-liquid ratio of adding water and mixing in step (1) is 1g: (4-8)ml; the water bath temperature is 80-90°C, and the time is 1-2h;

[0033] (b) in step (2), the protease is alkaline protease, the enzymolysis temperature is 40-45° C., and the enzymolysis time is 2-3 h;

[0034] (c) The centrifugation conditions in step (3) are both 3000-5000 r / min and centrifugation for 10-20 min.

[0035] The present invention finds that different extraction methods have an impact on grapefruit seed oil, and affect the synergistic effect of grapefruit seed oil and other components, affect the anti-inflammatory, antibacterial and sedative effects of the composition, and thus affect the overall comprehensive effect of the composition.

[0036] In a second aspect, the present invention provides use of the mild skin rejuvenating and highly effective acne removing composition as described in the first aspect in the preparation of cosmetics.

[0037] As a preferred embodiment of the second aspect, the cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

[0038] In a third aspect, the present invention provides a cosmetic comprising the exfoliating and anti-acne composition.

[0039] As a preferred embodiment of the third aspect, the cosmetic comprises the following components in percentage by mass: 1-3% of the exfoliating and anti-acne composition, 4-40% of the cosmetic base, and the balance water.

[0040] As a preferred embodiment of the third aspect, the cosmetic matrix includes at least one of a moisturizer, a thickener, a preservative, an emulsifier, a pH adjuster, and a flavor.

[0041] As a preferred embodiment of the third aspect, the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high molecular weight cellulose; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the pH adjuster includes at least one of arginine and EDTA-2Na.

[0042] Compared with the prior art, the beneficial effects of the present invention are:

[0043] The present invention finds that hydrolyzed prickly pear cactus flower extract, guaiac wood extract, grapefruit seed oil, and rose flower water have a synergistic effect with papain from numerous plant extracts, and the composition formed by specific compatibility has an effective effect in mild exfoliation, regulating water-oil balance, protecting skin barrier, and improving acne. The composition provided by the present invention can promote keratin exfoliation and promote natural replacement of keratin; effectively moisturize and replenish water, reduce excessive oil secretion; reduce inflammatory factors, protect skin barrier; improve skin acne, and improve skin health as a whole. The present invention provides a mild exfoliation and efficient acne removal composition and its application. The cosmetics containing the composition are mild and non-irritating to sensitive skin subjects, and have the effects of mild exfoliation, regulating water-oil balance, protecting skin barrier, and improving acne. It is suitable for sensitive skin, has a wide range of applications, and is highly accepted by consumers. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a picture showing the improvement of facial scales before and after using different lotions for 28 days;

[0045] Figure 2 This is a graph showing the improvement of facial acne on a human body before and after 28 days of using different lotions. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials. Further description of the raw materials used in the present invention:

[0047] The preparation method of grapefruit oil 1 is as follows:

[0048] (1) taking grapefruit seeds, crushing them, and drying them in an oven at 45° C. to obtain grapefruit seed powder;

[0049] (2) Weigh a certain amount of grapefruit seed powder, add distilled water at a solid-liquid ratio of 1 g:5 ml, stir thoroughly, and place in a 90°C water bath for 1 h;

[0050] (3) After cooling to room temperature, 1% (w / w) alkaline protease was added for enzymatic hydrolysis at 45°C for 2 h;

[0051] (4) After the enzymatic hydrolysis is completed, the mixture is centrifuged at 5000 r / min for 10 min to separate the emulsion layer for demulsification, and the emulsion layer is centrifuged again at 5000 r / min for 10 min for a second time to take the upper oil phase to obtain free oil;

[0052] (5) The free oil is placed in a drying oven and dried at 100° C. to a constant weight to obtain grapefruit seed oil 1.

[0053] The difference between the preparation method of grapefruit oil 2 and grapefruit oil 1 is as follows:

[0054] (a) The material-liquid ratio of water added and mixed in step (1) is 1 g:4 ml; the water bath temperature is 80° C., and the time is 1 h;

[0055] (b) In step (2), the enzymatic hydrolysis temperature is 50° C. and the enzymatic hydrolysis time is 2 h;

[0056] (c) In step (3), the centrifugation conditions for both times are 3000 r / min and centrifugation for 10 min.

[0057] The preparation method of grapefruit oil 3 differs from that of grapefruit oil 1 in the following aspects:

[0058] (a) The material-liquid ratio of water added and mixed in step (1) is 1 g:8 ml; the water bath temperature is 90° C. and the time is 2 h;

[0059] (b) In step (2), the enzymatic hydrolysis temperature is 40° C. and the enzymatic hydrolysis time is 3 h;

[0060] (c) In step (3), the centrifugation conditions for both times are 4000 r / min and centrifugation for 15 min.

[0061] The only difference between the preparation method of grapefruit oil 4 and the preparation method of grapefruit oil 1 is the use of cellulase.

[0062] The preparation method of grapefruit oil 5 is as follows:

[0063] Take grapefruit seeds, crush them, and place them in a 45℃ oven to dry them to obtain grapefruit seed powder. Weigh a certain amount of grapefruit seed powder, add 70% ethanol by volume at a solid-liquid ratio of 1g:5ml, and reflux extract at 80℃ for 1h. Centrifuge at 5000r / min for 10min, separate the emulsion layer for demulsification, and then centrifuge for the second time under the same conditions to obtain free oil, place it in a drying oven, and dry it at 100℃ to constant weight to obtain grapefruit seed oil-5.

[0064] Hydrolyzed Opuntia cactus flower extract: Product name is EEO cactus enzyme was purchased from Guangzhou Huayang Biotechnology Co., Ltd.;

[0065] Rose flower extract: The product name is rose flower extract, purchased from Xi'an Xirun Biotechnology Co., Ltd.;

[0066] Grape seed oil: The product name is grape seed oil, purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory;

[0067] Rosa centifolia flower water: the product name is Rosaliss TM , purchased from Ashland;

[0068] Centella asiatica floral water: the product name is Yanwen Centella asiatica floral water, purchased from Guangzhou Fanshu International Biotechnology Co., Ltd.;

[0069] Papain: The product name is papain, purchased from Beijing Solaibao Technology Co., Ltd.;

[0070] Citric acid: The product name is citric acid (≥99.5%), purchased from Bio-Technology Co., Ltd.;

[0071] Guaiac wood extract: Product number: L2-DZ-SCB3675-X, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0072] Calendula officinalis extract: The product name is calendula officinalis extract, purchased from Guangzhou Meiyi Biotechnology Co., Ltd.

[0073] The preparation method of the mild skin rejuvenating and highly effective acne removing composition of the present invention comprises the following steps: weighing components in parts by weight, stirring and mixing evenly, and ultrasonicating for 10 minutes to obtain the mild skin rejuvenating and highly effective acne removing composition.

[0074] Examples and Comparative Examples

[0075] The present application provides a composition for gentle skin rejuvenation and efficient acne removal, and the components and weight portions of the composition are shown in Table 1.

[0076] Table 1:

[0077]

[0078]

[0079] Effect example: Keratinocyte renewal test

[0080] This effect example tests the effects of the compositions of the examples and comparative examples in promoting cell renewal:

[0081] 1. Experimental materials:

[0082] Phosphate buffered saline (PBS), Shanghai Solebao; MTT, Yuanye Biotechnology; dimethyl sulfoxide (DMSO), Tianjin Damao; high glucose medium 1×DMEM, Gibco; fetal bovine serum (FBS), Gibco; keratinocytes were purchased from Guangzhou Customs Technology Center; keratinocytes were purchased from Kunming Cell Bank, Chinese Academy of Sciences.

[0083] 2. Experimental steps:

[0084] The cell suspension was inoculated into a 96-well cell culture plate and returned to the incubator for 18-24 hours. The complete culture medium in the well was aspirated, PBS / solvent was added, and the plate was exposed to UVB light (irradiation intensity: 80-90 μW / cm 2 , irradiation dose: 80mJ / cm 2 ), the different groups of UVB light treatments are as follows:

[0085] Blank control group: No irradiation, complete medium was added to the blank control group and cultured for 24 hours;

[0086] Experimental group: After irradiation for 15 min, the PBS solution was aspirated, and complete culture medium was added to the experimental group. 100 μL of 0.5% test sample (composition of Examples 1-16 and Comparative Examples 1-12) was added to each well and cultured for 24 h;

[0087] Control group: After irradiation for 15 min, PBS or solvent was added to the control group and cultured for 24 h.

[0088] Model group: After irradiation for 15 min, the PBS solution was aspirated, and complete culture medium was added to the experimental group and cultured for 24 h.

[0089] Take out the culture plate, add 20 μL of thiazolyl blue (MTT) solution to each well (MTT can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit it in the cells, while dead cells do not have this function), and incubate in the incubator for 3-4 hours. Remove the liquid in the wells, add 100 μL of DMSO to each well (DMSO can dissolve the formazan in the cells, and its light absorption value can be measured at a wavelength of 570nm using an enzyme-linked immunosorbent assay, which can indirectly reflect the number of living cells), place it on an oscillator for 10-15 minutes, and then measure the absorbance OD value at a wavelength of 570nm on the microplate reader, and calculate the cell viability. All experiments were repeated at least three times according to the principle of parallel design.

[0090] The experimental data were expressed as mean ± SD, and the T test was used for data comparison and analysis. Compared with the blank control, # is indicated, #P<0.05, significant; ##P<0.01, significantly significant;

[0091] ###P<0.001, very significant. Compared with the model group, * is used to indicate that *P<0.05 is significant; **P<0.01 is significantly significant; ***P<0.001 is very significant. The cell viability calculation formula is as follows:

[0092] Keratinocyte viability % = (OD value sample well - OD value zeroed) / (OD value blank control well - OD value zeroed) × 100%; wherein, the greater the keratinocyte viability (%), the stronger the effect of the combination in promoting keratin renewal.

[0093] Table 2:

[0094] Group Keratinocyte viability (%) Group Keratinocyte viability (%) Blank control group 100.00 Model Group <![CDATA[58.70 ## ]]> Example 1 71.45* Comparative Example 1 61.83 Example 2 75.23* Comparative Example 2 62.05 Example 3 78.78** Comparative Example 3 55.20 Example 4 79.23** Comparative Example 4 56.31 Example 5 81.01** Comparative Example 5 55.53 Example 6 82.23** Comparative Example 6 55.90 Example 7 86.45** Comparative Example 7 56.20 Example 8 83.67** Comparative Example 8 59.53 Example 9 83.10** Comparative Example 9 58.90 Example 10 86.52** Comparative Example 10 58.75 Embodiment 11 82.34** Comparative Example 11 59.23 Example 12 88.78** Comparative Example 12 58.01 Example 13 82.56** Embodiment 14 81.45** Embodiment 15 81.12** Example 16 80.21**

[0095] As can be seen from Table 1, the mild skin rejuvenating and highly effective acne removing composition provided by the present invention can inhibit the death of keratinocytes after UV irradiation, promote the formation of new keratinocytes, and effectively enhance the ability of keratinocyte renewal, which indicates that the combination of the present invention has the effect of promoting keratin renewal.

[0096] As can be seen from Examples 1-5, the mass ratio of the components in the composition of the present invention can affect the ability of the composition to promote keratinocyte renewal. When the mass parts of the components are limited to the mass ratio of component A, component B and component C (1.1-6): (1.01-11): (0.01-0.1), the obtained composition has a good keratin renewal effect, and when the mass ratio of component A, component B and component C is further selected to be within the range of (1.5-4): (2.05-8.8): (0.04-0.08), the obtained composition has a better effect of promoting keratin metabolism, and when the mass ratio of component A, component B and component C is 2: 6.7: 0.06 (Example 5), it has the best effect of promoting keratin metabolism.

[0097] The present invention studies the mass ratio of the hydrolyzed pear cactus flower extract and the guaiac extract in component A through Examples 6-8. It can be seen from Examples 6-8 that the mass ratio of the hydrolyzed pear cactus flower extract and the guaiac extract affects the keratinocyte viability of the composition, thereby affecting the ability of the composition to promote keratin renewal. Therefore, when the mass ratio of the hydrolyzed pear cactus flower extract and the guaiac extract is limited to the range of (0.1-3): (1-3), the ability of the composition to promote keratin renewal can be improved. When the mass ratio of the hydrolyzed pear cactus flower extract and the guaiac extract is further selected to be within the range of (0.3-2): (1.2-2), the effect of the obtained composition is better. When the mass ratio of the hydrolyzed pear cactus flower extract and the guaiac extract is 0.5: 1.5 (Example 7), the effect of promoting keratin renewal is best.

[0098] The present invention studies the mass ratio of grapefruit seed oil to Rosa centifolia flower water through Examples 9-11. It can be seen from Examples 9-11 that the mass ratio of grapefruit seed oil to Rosa centifolia flower water affects the keratinocyte viability of the composition, thereby affecting the ability of the composition to promote keratin renewal. Therefore, when the mass ratio of grapefruit seed oil to Rosa centifolia flower water is limited to the range of (0.01-1): (1-10), the ability of the composition to promote keratin can be improved. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is further selected to be within the range of (0.05-0.8): (2-8), the effect of the obtained composition is better. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is 0.4:6.3 (Example 10), its keratin removal effect reaches the best.

[0099] In summary, the composition with the best distribution ratio of each group was obtained: hydrolyzed Opuntia ficus-indica flower extract: guaiac wood extract: grapefruit seed oil: Rosa centifolia flower water: papain = 0.5:1.5:0.4:6.3:0.06 (Example 12).

[0100] The present invention studies the effect of grapefruit seed oil obtained by different extraction methods on the exfoliation ability of the composition through Examples 12-16. It can be seen from Application Examples 12-16 that the extraction method of grapefruit seed oil has a certain effect on the exfoliation effect. When grapefruit seed oil 1 is used, the exfoliation effect of the composition obtained is the best (Example 12), and the exfoliation effects of Examples 13-16 using other extraction methods are not as good as Example 12.

[0101] It can be seen from Comparative Examples 1-2 that the exfoliating effect of Comparative Examples 1-2 is poor. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0102] It can be seen from Comparative Examples 3-7 that the components in the composition provided by the present invention are indispensable. When any one of them is missing, the keratinocyte activity of the obtained composition is significantly reduced.

[0103] It can be seen from Comparative Examples 8-12 that when other similar ingredients are used to replace the components in the composition of the present invention, the corresponding effects of the present invention cannot be achieved.

[0104] Application Examples

[0105] The application examples of the present invention and the comparative application examples provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 3; wherein the mild skin rejuvenating and efficient acne-removing compositions used in application examples 1-16 are respectively the compositions prepared in Examples 1-16, and the mild skin rejuvenating and efficient acne-removing compositions used in application examples 17 and 18 are respectively the compositions prepared in Example 1; the mild skin rejuvenating and efficient acne-removing compositions used in comparative application examples 1-12 are respectively the compositions prepared in comparative examples 1-12; in addition, a blank application example is set, and the only difference between the blank application example and application example 1 is that the mild skin rejuvenating and efficient acne-removing compositions are not added; see Table 3 for details:

[0106] Table 3:

[0107]

[0108]

[0109] The preparation method of the emulsion in Application Example 1 is:

[0110] (1) Mix phase A and water, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep the mixture warm for later use to obtain prefabricated phase A;

[0111] (2) Mix phase B, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep warm for later use to obtain prefabricated phase B;

[0112] (3) Mix phase C and heat to 60°C to melt to obtain prefabricated phase C;

[0113] (4) The preformed phase A is heated to 80°C, and the preformed phase B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed phase C is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to below 45°C, and the anti-inflammatory and anti-acne composition and essence are added, and stirring is continued for 5 minutes. Finally, arginine is added to adjust the pH to 5.0-6.5, and stirring can be stopped. The material is discharged to obtain an emulsion.

[0114] Test example: Testing the skin scaling index, TEWL, moisturizing degree, and acne count of each lotion in the application example

[0115] This test examined the improvement effects of Application Examples 1-18, Comparative Application Examples 1-12, and Blank Application Examples on human skin TEWL, roughness, and acne.

[0116] The specific test method is as follows:

[0117] According to the "Technical Specifications for Safety of Cosmetics" (2015), 155 volunteers aged 18-50 with sensitive skin (lactic acid stinging score>3) were selected and randomly divided into 31 groups, 5 people in each group, and the volunteers had obvious acne. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days; every morning and evening, after cleansing and moisturizing, the volunteers took an appropriate amount of lotion and applied it to the face, massaged it for absorption, and the acne area could be overlapped. The whole face was used once in the morning and evening, and a follow-up visit was made every day. On the day of the volunteer's visit, the test area washed its face with clean water without applying any product, and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes.

[0118] Instruments were used to quantify and photograph the improvement of facial acne indicators, including acne count (classification count: comedones, papules, using VISIA and doctor assessment respectively), Tewameter TM Hex test of transepidermal water loss TEWL, CorneometerCM 825 test of moisturizing, and VC 20Plus test of scaling index.

[0119] The experimental data are expressed as mean ± SD, and the T test is used for data comparison and analysis. Compared with the blank control, * is indicated, *P < 0.05, significant; **P < 0.01, significantly significant; ***P < 0.001, very significantly significant. The improvement rate before and after use % = (X before use mean - X after use mean) / X before use mean × 100%;

[0120] Among them: the greater the improvement rate of the scale index %, the stronger the exfoliating ability of the lotion; the greater the improvement rate of papules and acne %, the stronger the acne removal ability of the lotion; the greater the improvement rate of the TEWL value, the stronger the skin barrier damage repair ability of the lotion; the greater the improvement rate of moisturizing %, the stronger the moisturizing and hydrating ability of the lotion. The calculation results are shown in Table 4:

[0121] Table 4:

[0122]

[0123]

[0124] As can be seen from Table 4, the emulsion provided by the present invention has excellent mild exfoliation, skin barrier repair, acne removal, and moisturizing effects. Specifically, on the 28th day, the obtained scaling index improvement rate was more than 12%, the TEWL value improvement rate was more than 8%, the papule improvement rate was more than 17%, the acne improvement rate was more than 12%, and the moisturizing rate was more than 25%. It can be seen from Application Examples 1-12 that the mass fraction of the components in the exfoliating and acne-removing composition added to the emulsion will affect the comprehensive performance of the product.

[0125] It can be seen from Application Examples 1-5 that the mass ratio of each component of the composition in the emulsion will affect the scale index, TEWL value, papule acne improvement and moisturizing ability of the composition. When the mass ratio of the components is limited to the mass ratio of component A, component B and component C (1.1-6): (1.01-11): (0.01-0.1), the obtained composition can reduce the scale index of the skin, improve the TEWL value, papule acne improvement rate and the moisturizing ability of the emulsion, and improve the overall comprehensive effect of the emulsion. When the mass ratio of the components A, component B and component C is further selected to be within the range of (1.5-4): (2.05-8.8): (0.04-0.08), the effect of the obtained composition is better. When the mass ratio of component A, component B and component C is 2: 6.7: 0.06 (Application Example 5), the overall effect is the best.

[0126] The present invention studies the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract in component A through application examples 6-8. It can be seen from application examples 6-8 that the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract affects the TEWL value and the moisturizing improvement rate of the emulsion, thereby affecting the barrier repair and moisturizing ability of the emulsion. Therefore, when the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is limited to the range of (0.1-3): (1-3), the barrier repair and moisturizing ability of the emulsion can be improved. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is further selected to be within the range of (0.3-2): (1.2-2), the effect of the obtained emulsion is better. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is 0.5:1.5 (application example 7), the barrier repair and moisturizing ability effect is best.

[0127] The present invention studies the mass ratio of grapefruit seed oil to Rosa centifolia flower water through application examples 9-11. It can be seen from application examples 9-11 that the mass ratio of grapefruit seed oil to Rosa centifolia flower water affects the improvement rate of papules and acne of the emulsion, thereby affecting the acne removal ability of the emulsion. Therefore, when the mass ratio of grapefruit seed oil to Rosa centifolia flower water is limited to the range of (0.01-1): (1-10), the acne removal ability of the emulsion can be improved. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is further selected to be within the range of (0.05-0.8): (2-8), the effect of the obtained emulsion is better. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is 0.4:6.3 (application example 10), the acne removal effect is best.

[0128] In summary, the composition with the best distribution ratio of each group was obtained: hydrolyzed prickly pear cactus flower extract: guaiac wood extract: grapefruit seed oil: Rosa centifolia flower water: papain = 0.5:1.5:0.4:6.3:0.06 (Application Example 12).

[0129] The present invention studies the influence of grapefruit seed oil obtained by different extraction methods on the acne removal ability of the composition through application examples 12-16. It can be seen from application examples 12-16 that the extraction method of grapefruit seed oil has a certain influence on the acne removal effect, thereby affecting the acne removal efficacy of the composition. When grapefruit seed oil 1 is used, the anti-inflammatory effect of the composition obtained is the best (application example 12), and the acne removal effects of application examples 13-16 using other extraction methods are not as good as application example 12.

[0130] It can be seen from Application Example 1 and Comparative Application Examples 1-2 that the moisturizing, acne-removing, exfoliating and skin barrier repair effects of Comparative Application Examples 1-2 are poor. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0131] It can be seen from Application Example 1 and Comparative Application Examples 3-7 that the components in the composition of the emulsion provided by the present invention are indispensable, and when any one of them is missing, the comprehensive performance of the obtained emulsion is significantly reduced.

[0132] It can be seen from Application Example 1 and Comparative Application Examples 8-12 that when other similar ingredients are used to replace the components in the composition of the present invention, the corresponding effects of the present invention cannot be achieved.

[0133] Among them, the photographs of volunteers before and after using the emulsion in the blank application example, application example 1 and comparative application example 8 are as follows: Figure 1 , 2 As shown, from Figure 1 It can be seen intuitively that the emulsion provided by the present invention can effectively improve human facial squama (the less red part in the figure, the better the squama improvement effect), achieving the purpose of exfoliation; Figure 2 It can be seen intuitively that the emulsion provided by the present invention can effectively improve human facial acne and achieve the purpose of removing acne.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for gentle skin rejuvenation and efficient acne removal, characterized in that: The composition comprises component A, component B and component C; The component A comprises hydrolyzed opuntia cactus flower extract and guaiac extract; The component B includes grapefruit seed oil and rose flower water; The component C is papain.

2. The composition according to claim 1, characterized in that The mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.1-6): (1.01-11): (0.01-0.1).

3. The composition according to claim 2, characterized in that The mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.5-4): (2.05-8.8): (0.04-0.08).

4. The composition according to claim 1, characterized in that In the component A, the mass ratio of the hydrolyzed opuntia flower extract to the guaiac extract is: the hydrolyzed opuntia flower extract: the guaiac extract = (0.1-3): (1-3).

5. The composition according to claim 4, characterized in that In the component A, the mass ratio of the hydrolyzed opuntia flower extract to the guaiac extract is: the hydrolyzed opuntia flower extract: the guaiac extract = (0.3-2): (1.2-2).

6. The composition according to claim 1, characterized in that In the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water=(0.01-1):(1-10).

7. The composition according to claim 6, characterized in that In the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water=(0.05-0.8):(2-8).

8. The composition according to any one of claims 1 to 7, characterized in that The preparation method of the grapefruit seed oil comprises the following steps: (1) taking grapefruit seeds, drying them, crushing them, adding water and mixing them in a water bath; (2) adding protease to perform enzymolysis to obtain an enzymolysis product; (3) centrifuging the enzymatic hydrolyzate to break the emulsion, separating the emulsion layer, centrifuging the emulsion layer, taking the upper oil phase, and obtaining free oil; (4) drying the free oil to a constant weight to obtain the grapefruit seed oil.

9. The composition according to claim 8, characterized in that Satisfy at least one of the following: (a) the material-liquid ratio of adding water and mixing in step (1) is 1g: (4-8)ml; the water bath temperature is 80-90°C, and the time is 1-3h; (b) in step (2), the protease is alkaline protease, the enzymolysis temperature is 40-45° C., and the enzymolysis time is 2-3 h; (c) The centrifugation conditions in step (3) are both 3000-5000 r / min and centrifugation for 10-20 min.

10. Use of the composition according to any one of claims 1 to 9 in the preparation of cosmetics.

11. The use according to claim 10, characterized in that The cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

12. A cosmetic, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 9.

13. The cosmetic according to claim 12, characterized in that The cosmetic comprises the following components in percentage by mass: 1-5% of the composition according to any one of claims 1 to 9, 4-40% of a cosmetic base, and the balance being water.

14. The cosmetic according to claim 13, characterized in that The cosmetic matrix includes at least one of a moisturizer, a thickener, a preservative, an emulsifier, a pH adjuster, and a fragrance.

Images