Extraction process and product of skin care active ingredients from coffee peel

Abstract

The present invention relates to the field of plant extraction, and in particular to an extraction process for skin care active ingredients in coffee peels. The present invention adopts a natural and pollution-free coffee peel extraction method, and extracts the natural active chemical components in the coffee peels through enzymatic biomimetic extraction, which can achieve the simultaneous extraction of chlorogenic acid, caffeic acid, and resveratrol in the coffee peels. The obtained extraction product has a high content of skin care active ingredients, which can be used as a cosmetic raw material to exert anti-inflammatory, anti-oxidation, whitening, soothing and other skin care effects; this technology makes full use of the production waste of coffee peels, reduces environmental pollution, recycles resources, and improves economic benefits.

Description

Technical Field

[0001] The invention relates to the field of plant extraction, and in particular to an extraction process and product of skin care active ingredients in coffee peel. Background Art

[0002] Coffee peel is the main by-product produced in the coffee production process. It contains rich nutrients and plant active ingredients, such as polyphenols, flavonoids, organic acids, etc. At present, the comprehensive utilization of coffee peel is mainly reflected in food, feed, composting, etc., and the development of coffee peel resources is still insufficient. The plant active ingredients contained in coffee peel have strong antioxidant, antibacterial and anti-inflammatory effects. Among them, chlorogenic acid, caffeic acid, and resveratrol have been widely used as functional skin care ingredients in the cosmetics industry; however, the existing plant extraction methods have not been able to effectively extract and utilize these skin care ingredients from coffee peel.

[0003] Chinese patent CN107235840A provides a method for extracting chlorogenic acid and tannin from coffee beans, which combines ether degreasing with water extraction to obtain a large amount of lipid substances. Chinese patent CN111728069B provides a method for preparing coffee extract, which extracts coffee aroma extract products through gas-liquid countercurrent extraction, and then adds glycosidase for enzymolysis to obtain coffee extract with floral and fruity aromas.

[0004] However, these existing technologies have not achieved the simultaneous extraction of skin care ingredients from coffee peel waste, and the skin care effect of the extracted products is limited; therefore, there is an urgent need to provide an extraction process for coffee peel with a high extraction rate and a high content of active ingredients in the product. Summary of the invention

[0005] The present invention adopts a natural and pollution-free coffee peel extraction method. The natural active chemical components in the coffee peel are extracted by enzymatic biomimetic extraction, and chlorogenic acid, caffeic acid and resveratrol in the coffee peel can be extracted simultaneously. The obtained extraction product has a high content of skin care active ingredients and can be used as a cosmetic raw material to exert anti-inflammatory, antioxidant, whitening, soothing and other skin care effects. This technology makes full use of the production waste of coffee peel, reduces environmental pollution, recycles resources, and improves economic benefits.

[0006] A first aspect of the present invention provides a process for extracting skin care active ingredients from coffee peels, the extraction process comprising the following steps: S1. Mix coffee peels with water, adjust the pH to 4.0-6.0, then add the first complex enzyme, and stir to obtain a first mixture; S2. taking the first mixture for a first extraction, and then filtering and separating to obtain a first extract and a first extraction residue; S3. Take the first extraction residue and mix it with water, adjust the pH to 7.0-8.0, then add the second complex enzyme, stir evenly to obtain a second mixture; S4. Take the second mixture for a second extraction, and then filter and separate to obtain a second extract and a second extraction residue; S5. combining the first extract and the second extract, and concentrating under reduced pressure to obtain a concentrate; S6. treating the concentrated solution with a polar macroporous adsorption resin so that the target substance is loaded on the polar macroporous adsorption resin; S7. transferring the polar macroporous adsorption resin to a chromatography column for elution to obtain an eluate; S8. The eluate is pressurized, concentrated, and dried to obtain a coffee peel extract.

[0007] In some preferred embodiments, the added amount of the first complex enzyme is 1-3wt% of the total mass of the coffee peel, which can be listed as 1wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, 2wt%, 2.1wt%, 2.2wt%, 2.3wt%, 2.4wt%, 2.5wt%, and 3wt%.

[0008] In some preferred embodiments, the first complex enzyme comprises cellulase, pectinase and α-amylase.

[0009] More preferably, based on the total mass of the coffee peel, the first complex enzyme comprises 0.5-1% cellulase, 0.4-0.8% pectinase and 0.2-0.5% α-amylase; most preferably 0.9% cellulase, 0.6% pectinase and 0.4% α-amylase.

[0010] In some preferred embodiments, the added amount of the second complex enzyme is 0.2-2wt% of the total mass of the coffee peel, which can be listed as 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, and 2wt%.

[0011] In some preferred embodiments, the second complex enzyme comprises protease and lipase.

[0012] More preferably, based on the total weight of the coffee peel, the second complex enzyme comprises 0.5-1.5% protease and 0.1-0.3% lipase; most preferably, 0.8% protease and 0.2% lipase.

[0013] In some preferred embodiments, a buffer is used to adjust the pH in both step S1 and step S3; the buffer can be listed as a phosphate buffer system, an acetate buffer system, a borax-calcium chloride buffer system, an ammonia-ammonium chloride buffer system; preferably, an acetic acid-sodium acetate buffer.

[0014] In some preferred embodiments, the coffee peel is mixed with water in a mass ratio of 1:(5-15); more preferably 1:10.

[0015] In some preferred embodiments, the conditions for the first extraction are: extraction temperature 50-60°C, extraction time 10-15h, stirring during the extraction process; more preferably, the extraction temperature is 55°C, and the extraction time is 12h.

[0016] In some preferred embodiments, the first extraction residue is mixed with water in a mass ratio of 1:(5-15).

[0017] In some preferred embodiments, the conditions for the second extraction are: extraction temperature 35-45°C, extraction time 5-10h, stirring during the extraction process; more preferably, the extraction temperature is 40°C, and the extraction time is 8h.

[0018] The present invention uses a bionic enzymatic hydrolysis method to extract coffee peels, and realizes the simultaneous extraction of chlorogenic acid, caffeic acid and resveratrol. In the bionic enzymatic hydrolysis process, it is particularly important to set the pH and temperature of the extraction system. The first extraction process of the present invention needs to be controlled in a weak acid environment. After investigation, it is found that when the pH of the first extraction is set to 4.0-6.0, the extraction temperature is 50-60°C, and the extraction time is 10-15h; when combined with 0.5-1% cellulase, 0.4-0.8% pectinase and 0.2-0.5% α-amylase, the efficiency of breaking the plant cell wall is the highest. It is speculated that the biological activity of the enzyme is high under this condition, and the components in the cell are more fully released. The components such as chlorogenic acid in the coffee peel can also be stably present under this condition, which is convenient for subsequent extraction and purification, and finally improves the extraction rate and the content of active ingredients in the product. In order to further cooperate with the second step of enzymatic extraction, the second complex enzyme is selected as a combination of protease and lipase, and the first extraction filter residue is extracted again at a relatively low temperature (35-45°C) after the first extraction, which deeply decomposes the cell wall of the coffee peel. It can reduce energy consumption while extracting the active ingredients in the coffee peel waste to the greatest extent, thereby increasing the content of chlorogenic acid, caffeic acid and resveratrol in the final product.

[0019] In some preferred embodiments, the concentrated solution is treated with a polar macroporous adsorption resin by mixing the concentrated solution and the polar macroporous adsorption resin in a weight ratio of (1.5-2.5):1, and adsorbing by shaking at room temperature for 20-30 hours; more preferably, the concentrated solution and the polar macroporous adsorption resin are mixed in a weight ratio of 2:1, and adsorbing by shaking at room temperature for 24 hours.

[0020] In some preferred embodiments, the polar macroporous adsorption resin is selected from any one of resin D101, resin D301 or resin AB-8, preferably resin D101.

[0021] The material of resin D101 is mainly polystyrene non-polar adsorption resin, and the material of resin D301 is mainly styrene-divinylbenzene copolymer with tertiary amine group [-N(CH 3 ) 2 ] anion exchange resin, the material of resin AB-8 is mainly styrene, divinylbenzene, etc. as raw materials, a certain proportion of porogen is added to 0.5% gelatin solution and cross-linked and polymerized to form a macroporous adsorption resin porous skeleton structure. These resins can be obtained from the market, such as Anhui Sanxing Resin Technology Co., Ltd. or Hebei Baoen Resin Technology Co., Ltd.

[0022] In some preferred embodiments, the elution treatment comprises: first eluting impurities with water, then eluting with an organic solvent, and collecting the eluate.

[0023] More preferably, the elution treatment comprises: first eluting impurities with 5-15 column volumes of water, controlling the flow rate to 0.5-2 BV / h; then eluting with 4-6 column volumes of organic solvent, controlling the flow rate to 0.3-1.5 BV / h, and collecting the eluate.

[0024] Most preferably, the elution treatment comprises: first eluting impurities with 10 column volumes of water, controlling the flow rate to 1 BV / h; then eluting with 5 column volumes of organic solvent, controlling the flow rate to 0.8 BV / h, and collecting the eluate.

[0025] The organic solvent includes at least one of ethanol, acetone, ethyl acetate and chloroform; preferably ethanol.

[0026] In some preferred embodiments, the organic solvent is an ethanol aqueous solution, and the concentration of the ethanol aqueous solution is 50-70%, which can be listed as 50%, 55%, 60%, 65%, 70%; the preferred concentration is 60%; unit: v / v.

[0027] The present invention uses a specific secondary enzymatic extraction method to efficiently extract skin care active ingredients from coffee peels, but the extracted product here inevitably contains impurities; the present invention further uses a polar macroporous adsorption resin, and uses the high porosity structure of the polar macroporous adsorption resin and the polymer adsorbent with a large specific surface area to accurately screen the target active ingredients. Resin D101 is preferably used, and the active adsorption sites on its surface can interact with the polar molecules of chlorogenic acid, caffeic acid and resveratrol to achieve efficient adsorption and separation of the target active substances, and further cooperate with elution with ethanol aqueous solution to obtain a coffee peel extract product with a high content of active ingredients.

[0028] The present invention does not impose any particular limitation on the stirring speed, as long as the material can be stirred into a fluid state.

[0029] A second aspect of the present invention provides a product of a process for extracting skin care active ingredients from coffee peels, wherein the content of chlorogenic acid in the coffee peel extract is higher than 6wt%, and the content of resveratrol is higher than 5wt%.

[0030] More preferably, the content of caffeic acid in the coffee peel extract is higher than 2 wt %.

[0031] A third aspect of the present invention provides an application of a product of the extraction process of skin care active ingredients in coffee peel as described above in cosmetics, wherein the cosmetics include at least one of toner, essence, skin care lotion, face cream, eye cream, facial cleanser, facial mask, shampoo, shower gel, lip balm, and body lotion.

[0032] Beneficial effects: The present invention provides a process for extracting skin care active ingredients from coffee peel and a product thereof, which has the following advantages: (1) The present invention uses enzymatic biomimetic extraction of natural active chemical components in coffee peels, thereby improving the extraction rates of three active components, namely chlorogenic acid, caffeic acid and resveratrol, in the coffee peels. In addition, the solvent used in the extraction process is water, and no organic solvent (such as ethanol) is used for extraction, which can reduce pollution to the environment.

[0033] (2) It is preferred that the first complex enzyme and the second complex enzyme are extracted separately, and the temperature and time of the two extractions are controlled, thereby increasing the content of the three active ingredients, chlorogenic acid, caffeic acid and resveratrol, extracted from the coffee peel; it is particularly preferred that the first complex enzyme includes cellulase, pectinase and α-amylase, and the second complex enzyme includes protease and lipase, and they are arranged accordingly.

[0034] (3) The extraction process of the present invention has mild conditions and a low extraction temperature, which reduces the extraction cost. It can extract the active ingredients in the coffee peel residue waste to the greatest extent while reducing energy consumption, so that it can play a skin care effect.

[0035] (4) The crude coffee peel extract of the present invention is purified by polar macroporous adsorption resin, and in particular, resin D101 is used to enrich and purify the natural active chemical components, so that the contents of chlorogenic acid, caffeic acid and resveratrol in the extract are significantly increased. The obtained coffee peel extract provides high-quality raw materials for the development of skin care products.

[0036] (5) The bionic enzymatic extraction method of the present invention can fully decompose the components such as protein, fat, and sugar in coffee peel. The coffee peel residue after extraction is greatly reduced compared with the traditional solvent extraction method, which effectively realizes the recycling of resources and greatly reduces the pollution to the environment caused by the plant waste treatment process (such as burning, composting, etc.).

[0037] (6) The extraction process of the present invention is simple and easy to implement, and will not produce harmful substances to pollute the environment. It has the technical advantages of environmental protection and sustainability, can be mass-produced, and is easy to promote and implement. DETAILED DESCRIPTION

[0038] The coffee husk used in the embodiments of the present invention is produced in Yunnan.

[0039] Note: Unless otherwise specified, the solvents of the solutions involved in the present invention are all water; the room temperature is 25°C; and the raw materials used are all commercially available.

[0040] Example 1 This embodiment provides a process for extracting skin care active ingredients from coffee peels, the steps comprising: S1. Take 1 kg of coffee peel, mix it with pure water at a mass ratio of 1:10, adjust the pH to 5.0 using acetic acid-sodium acetate buffer, then add the first complex enzyme, and stir evenly to obtain a first mixture; Based on the total weight of the coffee peel, the first complex enzyme includes 0.9% cellulase, 0.6% pectinase and 0.4% α-amylase.

[0041] S2. Take the first mixed material and stir it at 55° C. for 12 hours, then filter and separate it through 400 mesh to obtain a first extract and a first extraction residue.

[0042] S3. The first extraction residue was mixed with pure water in a mass ratio of 1:10, the pH was adjusted to 7.5 using acetic acid-sodium acetate buffer, and then the second complex enzyme was added and stirred to obtain a second mixture; Based on the total weight of the coffee peel, the second complex enzyme includes 0.8% protease and 0.2% lipase.

[0043] S4. Take the second mixed material and stir and extract it at 40° C. for 8 hours, and then filter and separate it to obtain a second extract and a second extraction residue.

[0044] S5. Combine the first extract and the second extract, and concentrate under reduced pressure to obtain 2 kg of concentrated solution.

[0045] S6. Mix 2 kg of the concentrated solution with 1 kg of polar macroporous adsorption resin (resin D101, from Anhui Samsung Resin Technology Co., Ltd.), and adsorb under shaking at room temperature for 24 h to load the target substance onto the polar macroporous adsorption resin.

[0046] S7. Load the polar macroporous adsorption resin into the chromatography column, first elute the impurities with 10 column volumes of pure water, and control the flow rate of the elution process to be 1 BV / h; then elute with 5 column volumes of 60% (v / v) ethanol aqueous solution, and control the flow rate of the elution process to be 0.8 BV / h; collect the eluate.

[0047] S8. The eluate is pressurized, concentrated, and dried to obtain a coffee peel extract.

[0048] Comparative Example 1 This comparative example provides a process for extracting skin care active ingredients from coffee peels, specifically using a solvent reflux extraction method, the steps comprising: S1. Take 1 kg of coffee peel, mix it with pure water at a mass ratio of 1:10, adjust the pH to 5.0 using acetic acid-sodium acetate buffer, then add the first complex enzyme, and stir evenly to obtain a first mixture; Based on the total weight of coffee peel, the first complex enzyme includes 0.9% cellulase, 0.6% pectinase and 0.4% α-amylase.

[0049] S2. Take the first mixed material and stir it at 55° C. for 12 hours, then filter and separate it to obtain a first extract and a first extraction residue.

[0050] S3. The first extraction residue was mixed with pure water in a mass ratio of 1:10, the pH was adjusted to 7.5 using acetic acid-sodium acetate buffer, and then the second complex enzyme was added and stirred to obtain a second mixture; Based on the total weight of the coffee peel, the second complex enzyme includes 0.8% protease and 0.2% lipase.

[0051] S4. Take the second mixed material and stir and extract it at 40° C. for 8 hours, and then filter and separate it to obtain a second extract and a second extraction residue.

[0052] S5. Combine the first extract and the second extract, and concentrate under reduced pressure to obtain 2 kg of concentrated solution.

[0053] S6. The 2 kg concentrate was further concentrated under reduced pressure to 500 g concentrate, mixed with 4.5 kg 95% (v / v) ethanol aqueous solution, precipitated for 24 h, and the supernatant was collected by filtration; S7. Add 200 g of activated carbon to the supernatant collected in step S6, heat at room temperature for 2 h, decolorize and filter; S8. The supernatant obtained after filtering in step S7 is concentrated under reduced pressure to 500 g, and then steps S6 (secondary alcohol precipitation) and S7 (secondary decolorization) are repeated, and the filtrate obtained after filtering the secondary decolorization is concentrated to 1 kg, filtered, and concentrated under reduced pressure to obtain.

[0054] Comparative Example 2 This comparative example provides a process for extracting skin care active ingredients from coffee peels, the steps comprising: S1. Take 1 kg of coffee peel, mix it with pure water at a mass ratio of 1:10, adjust the pH to 8.0 using acetic acid-sodium acetate buffer, then add the first complex enzyme, and stir evenly to obtain a first mixture; Based on the total weight of the coffee peel, the first complex enzyme includes 3.0% cellulase, 0.8% pectinase and 0.6% α-amylase.

[0055] S2. Take the first mixed material and stir it at 70° C. for 6 hours, then filter and separate it to obtain a first extract and a first extraction residue.

[0056] S3. The first extraction residue was mixed with pure water in a mass ratio of 1:10, the pH was adjusted to 8.0 using acetic acid-sodium acetate buffer, and then the second complex enzyme was added and stirred to obtain a second mixture; Based on the total weight of the coffee peel, the second complex enzyme includes 1.0% protease and 0.6% lipase.

[0057] S4. Take the second mixed material and stir and extract it at 60° C. for 4 hours, and then filter and separate it to obtain a second extract and a second extraction residue.

[0058] S5. Combine the first extract and the second extract, and concentrate under reduced pressure to obtain 2 kg of concentrated solution.

[0059] S6. 2 kg of the concentrated solution and 1 kg of polar macroporous adsorption resin (resin D131, from Anhui Samsung Resin Technology Co., Ltd.) were mixed, and adsorbed under shaking at room temperature for 24 h to load the target substance on the polar macroporous adsorption resin.

[0060] S7. Load the polar macroporous adsorption resin into the chromatography column, first elute the impurities with 10 column volumes of pure water, and control the flow rate of the elution process to be 1 BV / h; then elute with 5 column volumes of 60% (v / v) ethanol aqueous solution, and control the flow rate of the elution process to be 0.8 BV / h; collect the eluate.

[0061] S8. The eluate is pressurized, concentrated, and dried to obtain a coffee peel extract.

[0062] Comparative Example 3 This comparative example provides a process for extracting skin care active ingredients from coffee peels, the steps comprising: S1. Take 1 kg of coffee peel, mix it with pure water at a mass ratio of 1:10, adjust the pH to 3.0 using acetic acid-sodium acetate buffer, then add the first complex enzyme, and stir evenly to obtain a first mixture; Based on the total weight of the coffee peel, the first complex enzyme includes 0.5% cellulase, 0.3% pectinase and 0.2% α-amylase.

[0063] S2. Take the first mixed material and stir it at 40° C. for 24 hours, then filter and separate it to obtain a first extract and a first extraction residue.

[0064] S3. The first extraction residue was mixed with pure water in a mass ratio of 1:10, the pH was adjusted to 3.0 using acetic acid-sodium acetate buffer, and then the second complex enzyme was added and stirred to obtain a second mixture; Based on the total weight of the coffee peel, the second complex enzyme includes 0.4% protease and 0.1% lipase.

[0065] S4. Take the second mixed material and stir and extract it at 30° C. for 10 hours, then filter and separate it to obtain a second extract and a second extraction residue.

[0066] S5. Combine the first extract and the second extract, and concentrate under reduced pressure to obtain 2 kg of concentrated solution.

[0067] S6. 2 kg of the concentrated solution and 1 kg of polar macroporous adsorption resin (resin AB-8, from Anhui Samsung Resin Technology Co., Ltd.) were mixed, and adsorbed under shaking at room temperature for 24 h to load the target substance on the polar macroporous adsorption resin.

[0068] S7. Load the polar macroporous adsorption resin into the chromatography column, first elute the impurities with 10 column volumes of pure water, and control the flow rate of the elution process to be 1 BV / h; then elute with 5 column volumes of 60% (v / v) ethanol aqueous solution, and control the flow rate of the elution process to be 0.8 BV / h; collect the eluate.

[0069] S8. The eluate is pressurized, concentrated, and dried to obtain a coffee peel extract.

[0070] Performance Testing Methods 1. Determination of chlorogenic acid and caffeic acid content The chromatographic conditions were as follows: C18 column, 0.4% phosphoric acid aqueous solution (A)-acetonitrile (B) as the mobile phase, gradient elution (0-10 min, 10%-12% B; 10-15 min, 12%-16% B; 15-20 min, 16%-19% B; 20-24 min, 19%-25% B; 24-31 min, 25%-25% B; 31-36 min, 25%-29% B; 36-60 min, 29%-50% B); detection wavelength: 320 nm; flow rate: 1.0 mL / min; injection volume: 20 µL; column temperature: 35°C.

[0071] 2. Resveratrol content determination method The chromatographic conditions were as follows: C18 column, methanol (A)-water (B) as the mobile phase, gradient elution (0-5 min, 40% B; 5-11 min, 10% B; 11-15 min, 0% B; 15-35 min, 65% B); detection wavelength: 306 nm; flow rate: 0.8 mL / min; injection volume: 10 µL; column temperature: 45°C.

[0072] 3. Preparation of standard and test sample solutions during the test Standard solution: accurately weigh 20 mg of chlorogenic acid, caffeic acid and resveratrol respectively and dissolve them in 80 ml of methanol, transfer them to a 100 ml volumetric flask, make up to volume, and prepare a 0.2 mg / ml standard solution.

[0073] Preparation of test sample solution: Take 2g of coffee peel extract of Example 1 and Comparative Examples 1-3, add 20ml of methanol, ultrasonically treat for 15min, and filter; add 20ml of methanol to the filter residue, ultrasonically treat for 15min, and filter. Combine the two filtrates, transfer to a 100ml volumetric flask, and adjust the volume; test on the machine. Among them, Example 1 was repeated 3 times for testing, recorded as experimental group 1, experimental group 2 and experimental group 3; Comparative Example 1 was repeated 3 times for testing, recorded as comparison group 1, comparison group 2 and comparison group 3.

[0074] Performance Test Results The extraction products of Example 1 of the present invention and Comparative Examples 1-3 were subjected to qualitative and quantitative analysis of the three active ingredients of chlorogenic acid, caffeic acid and resveratrol, and the extraction rates and contents of the three active ingredients were calculated. At the same time, the weight of the coffee peel residue waste obtained after extraction was recorded. The measurement results are shown in Table 1.

[0075] The extraction rate is the mass ratio of the component in the coffee peel extract to the coffee peel; the content is the mass ratio of the component in the coffee peel extract to the coffee peel extract. The extraction residue is the weight of the filter residue after extraction after drying at 95°C.

[0076] Table 1

[0077] As shown in Table 1, the present invention uses a bionic enzymatic hydrolysis method to extract coffee peels, which can significantly improve the extraction rate of chlorogenic acid, caffeic acid and resveratrol, and the crude product is purified and enriched by macroporous resin purification, and the content of active ingredients in coffee peel extraction is significantly increased. The obtained coffee peel extract can be used as a cosmetic raw material, which is more conducive to later applications. The extraction method of the present invention needs to control specific enzymatic extraction conditions and adsorption and purification conditions. When the pH setting, temperature setting and other conditions of the extraction process exceed the preferred range, or the traditional solvent reflux extraction method is used, similar technical effects cannot be achieved. In addition, after extraction by the process of the present invention, the solid matter in the coffee peel is degraded in large quantities, and the residue produced after extraction is reduced by about 40% compared with traditional solvent extraction, which avoids the pollution of the environment by coffee production waste. This technology is green and environmentally friendly and can achieve effective reuse of resources.

Claims

1. A process for extracting skin care active ingredients from coffee peel, characterized in that the steps include: S1. Mix coffee peels with water, adjust the pH to 4.0-6.0, then add the first complex enzyme, and stir to obtain a first mixture; S2. taking the first mixture for a first extraction, and then filtering and separating to obtain a first extract and a first extraction residue; S3. Take the first extraction residue and mix it with water, adjust the pH to 7.0-8.0, then add the second complex enzyme, stir evenly to obtain a second mixture; S4. Take the second mixture for a second extraction, and then filter and separate to obtain a second extract and a second extraction residue; S5. combining the first extract and the second extract, and concentrating under reduced pressure to obtain a concentrate; S6. treating the concentrated solution with a polar macroporous adsorption resin so that the target substance is loaded on the polar macroporous adsorption resin; S7. transferring the polar macroporous adsorption resin to a chromatography column for elution to obtain an eluate; S8. concentrating and drying the eluate to obtain a coffee peel extract; The first complex enzyme includes cellulase, pectinase and α-amylase; The second complex enzyme includes protease and lipase.

2. The process for extracting skin care active ingredients from coffee peel according to claim 1, characterized in that: Based on the total weight of the coffee peel, the first complex enzyme includes 0.5-1% cellulase, 0.4-0.8% pectinase and 0.2-0.5% α-amylase.

3. The process for extracting skin care active ingredients from coffee peel according to claim 1, characterized in that: Based on the total weight of the coffee peel, the second complex enzyme comprises 0.5-1.5% protease and 0.1-0.3% lipase.

4. The process for extracting skin care active ingredients from coffee peel according to claim 1, characterized in that: The coffee peels are mixed with water in a mass ratio of 1:(5-15).

5. The process for extracting skin care active ingredients from coffee peel according to claim 2, characterized in that: The conditions of the first extraction are: extraction temperature 50-60° C., extraction time 10-15 h, and stirring during the extraction process.

6. The process for extracting skin care active ingredients from coffee peel according to claim 3, characterized in that: The conditions of the second extraction are: extraction temperature 35-45° C., extraction time 5-10 h, and stirring during the extraction process.

7. The process for extracting skin care active ingredients from coffee peel according to claim 1, characterized in that: The treatment of the concentrated solution with the polar macroporous adsorption resin is specifically as follows: the concentrated solution and the polar macroporous adsorption resin are mixed in a weight ratio of (1.5-2.5):1, and adsorbed under shaking at room temperature for 20-30 hours; The polar macroporous adsorption resin is selected from any one of resin D101, resin D301 or resin AB-8.

8. The process for extracting skin care active ingredients from coffee peel according to claim 1, characterized in that: The elution treatment comprises: firstly eluting impurities with 5-15 column volumes of water, controlling the flow rate to be 0.5-2 BV / h; then eluting with 4-6 column volumes of organic solvent, controlling the flow rate to be 0.3-1.5 BV / h, and collecting the eluate.

9. A product of the process for extracting skin care active ingredients from coffee peel according to any one of claims 1 to 8, characterized in that: The content of chlorogenic acid in the coffee peel extract is higher than 6wt%, and the content of resveratrol is higher than 5wt%.

10. Use of the product of the extraction process of skin care active ingredients in coffee peel according to claim 9 in cosmetics, characterized in that: The cosmetics include at least one of toner, essence, skin care lotion, face cream, eye cream, facial cleanser, facial mask, shampoo, shower gel, lip balm, and body lotion.

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